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https://www.readbyqxmd.com/read/28432959/molecular-dynamics-simulation-studies-of-novel-%C3%AE-lactamase-inhibitor
#1
Farhan Ul Haq, Asma Abro, Saad Raza, Klaus R Liedl, Syed Sikander Azam
New Delhi Metallo-β-Lactamase-1 (NDM-1) has drawn great attention due to its diverse antibiotic resistant activity. It can hydrolyze almost all clinically available β-lactam antibiotics. To inhibit the activity of NDM-1 a new strategy is proposed using computational methods. Molecular dynamics (MD) simulations are used to analyze the molecular interactions between selected inhibitor candidates and NDM-1 structure. The enzyme-ligand complex is subject to binding free energy calculations using MM(PB/GB)SA methods...
April 4, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28411227/fate-and-persistence-of-a-pathogenic-ndm-1-positive-escherichia-coli-strain-in-anaerobic-and-aerobic-sludge-microcosms
#2
David Mantilla-Calderon, Pei-Ying Hong
The presence of emerging biological pollutants in treated wastewater effluents has gained attention due to increased interest in water reuse. To evaluate the effectiveness of the removal of such contaminants by the conventional wastewater treatment process, the fate and decay kinetics of NDM-1-positive Escherichia coli strain PI7 and its plasmid-encoded antibiotic resistance genes (ARGs) were assessed in microcosms of anaerobic and aerobic sludge. Results showed that E. coli PI7 decayed at a significantly slower rate under anaerobic conditions...
April 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28404680/development-and-validation-of-a-lateral-flow-immunoassay-for-the-rapid-detection-of-ndm-producing-enterobacteriaceae
#3
Hervé Boutal, Thierry Naas, Karine Devilliers, Saoussen Oueslati, Laurent Dortet, Sandrine Bernabeu, Stéphanie Simon, Hervé Volland
The global spread of carbapenemase-producing Enterobacteriaceae (CPE) that are often resistant to most, if not all, classes of antibiotics is a major public health concern. NDM-1 carbapenemase is among the most worrisome carbapenemases given its rapid worldwide spread. We have developed and evaluated a lateral flow immunoassay (LFIA, called NDM LFIA) for rapid and reliable detection of NDM-like carbapenemase-producing Enterobacteriaceae from culture colonies.We evaluated the NDM LFIA using 175 reference enterobacterial isolates with characterized ß-lactamase gene content and 74 non-duplicate consecutive carbapenem-resistant clinical isolates referred for expertise to the French National Reference Center (NRC) for antibiotic resistance during a one-week period (in June 2016)...
April 12, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28395597/genome-sequencing-and-comparative-analysis-of-an-ndm-1-producing-klebsiella-pneumoniae-st15-isolated-from-a-refugee-patient
#4
Tamara Salloum, Harout Arabaghian, Sahar Alousi, Edmond Abboud, Sima Tokajian
The escalating problem of antibiotic resistance, specifically cabarpenemase and extended-spectrum β-lacatamase (ESBL) producing K. pneumoniae strains, is directly correlated with increased patient morbidity and mortality and prolonged hospitalization and costs. In this study, a comprehensive genomic analysis encompassing the resistomics, virulence repertoire and mobile genetic elements of an NDM-1 positive ESBL-producing K. pneumoniae EA-MEH ST15 isolated from a urine sample collected from a Syrian refugee was conducted...
April 11, 2017: Pathogens and Global Health
https://www.readbyqxmd.com/read/28357923/performance-of-carbanp-and-cim-tests-in-oxa-48-carbapenemase-producing-enterobacteriaceae
#5
Serap Süzük Yıldız, Banu Kaşkatepe, Havva Avcıküçük, Şükran Öztürk
This study applied two phenotypic tests, namely "Carbapenemase Nordmann-Poirel" (CarbaNP) test and "Carbapenem Inactivation Method" (CIM), against the isolates carrying the carbapenem resistance genes. The study included 83 carbapenem-resistant Enterobacteriaceae isolates producing oxacillinase-48 (OXA-48) and 30 carbapenem-sensitive Enterobacteriaceae isolates. Out of the total isolates studied, 77 isolates (92.77%) were identified as Klebsiella pneumoniae and six isolates (7.23%) were identified as Escherichia coli by Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry...
March 1, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28333234/horizontal-transfer-of-the-blandm-1-gene-to-pseudomonas-aeruginosa-and-acinetobacter-baumannii-in-biofilms
#6
Windy D Tanner, Robyn M Atkinson, Ramesh K Goel, Mark A Toleman, Lowell Scott Benson, Christina A Porucznik, James A VanDerslice
Horizontal gene transfer has contributed to the global spread of the blaNDM-1 gene. Multiple studies have demonstrated plasmid transfer of blaNDM-1 between Gram-negative bacteria, primarily Enterobacteriaceae species, but conjugational transfer of natural blaNDM-1 plasmids from Enterobacteriaceae into Pseudomonas aeruginosa and Acinetobacter baumannii has not previously been shown. As P. aeruginosa and A. baumannii are both typically strong biofilm-formers, transfer of natural blaNDM-1 plasmids could potentially occur more readily in this environment...
February 25, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28319394/a-network-of-conformational-transitions-in-the-apo-form-of-ndm-1-enzyme-revealed-by-md-simulation-and-a-markov-state-model
#7
Kaifu Gao, Yunjie Zhao
New Delhi metallo-β-lactamase-1 (NDM-1) is a novel β-lactamase enzyme that confers enteric bacteria with nearly complete resistance to all β-lactam antibiotics, so it raises a formidable and global threat to human health. However, the binding mechanism between apo-NDM-1 and antibiotics as well as related conformational changes remains poorly understood, which largely hinders the overcoming of its antibiotic resistance. In our study, long-time conventional molecular dynamics simulation and Markov state models were applied to reveal both the dynamical and conformational landscape of apo-NDM-1: the MD simulation demonstrates that loop L3, which is responsible for antibiotic binding, is the most flexible and undergoes dramatic conformational changes; moreover, the Markov state model built from the simulation maps four metastable states including open, semiopen, and closed conformations of loop L3 as well as frequent transitions between the states...
March 30, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28303191/vim-1-carbapenemase-producing-escherichia-coli-in-gulls-from-southern-france
#8
Marion Vittecoq, Chrislène Laurens, Lionel Brazier, Patrick Durand, Eric Elguero, Audrey Arnal, Frédéric Thomas, Salim Aberkane, Nicolas Renaud, Franck Prugnolle, Jérôme Solassol, Hélène Jean-Pierre, Sylvain Godreuil, François Renaud
Acquired carbapenemases currently pose one of the most worrying public health threats related to antimicrobial resistance. A NDM-1-producing Salmonella Corvallis was reported in 2013 in a wild raptor. Further research was needed to understand the role of wild birds in the transmission of bacteria resistant to carbapenems. Our aim was to investigate the presence of carbapenem-resistant Escherichia coli in gulls from southern France. In 2012, we collected 158 cloacal swabs samples from two gull species: yellow-legged gulls (Larus michahellis) that live in close contact with humans and slender-billed gulls (Chroicocephalus genei) that feed at sea...
February 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28284092/antibiotic-resistant-pseudomonas-spp-in-the-aquatic-environment-a-prevalence-study-under-tropical-and-temperate-climate-conditions
#9
Naresh Devarajan, Thilo Köhler, Periyasamy Sivalingam, Christian van Delden, Crispin K Mulaji, Pius T Mpiana, Bastiaan W Ibelings, John Poté
Microbial populations which are resistant to antibiotics are an emerging environmental concern with potentially serious implications for public health. Thus, there is a growing concern in exploring the occurrence of antibiotic resistance in the environment with no limitations to the factors that contribute to their emergence. The aquatic environment is considered to be a hot-spot for the acquisition and spread of antibiotic resistance due to pollution with emerging contaminants derived from anthropogenic activities...
February 27, 2017: Water Research
https://www.readbyqxmd.com/read/28271693/comparison-of-the-novel-oxa-48-and-kpc-k-set-assay-and-blue-carba-test-for-the-detection-of-carbapenemase-producing-enterobacteriaceae-using-pcr-as-a-reference-method
#10
Fatma Erdem, Ayham Abulaila, Zerrin Aktas, Oral Oncul
BACKGROUND: Rapid, simple, and accurate laboratory detection of carbapenemases is very important for proper antibiotic therapy and infection control. METHODS: In this study, carbapenem-nonsusceptible Enterobacteriaceae (CRE) isolates were used to evaluate the performance of a new lateral flow immunochromatographic (IC) assay, the OXA-48 and KPC K-SeT assay, and modified Blue-Carba test (BCT) for the rapid detection of OXA-48 carbapenemase in comparison with polymerase chain reaction (PCR) amplification...
March 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28264751/characterization-of-transformants-obtained-from-ndm-1-producing-enterobacteriaceae-in-brazil
#11
Franciéli P Rozales, Cibele M Magagnin, Juliana C Campos, Mariana Pagano, Luciana S Nunes, Lisiane R Pancotto, Jorge L M Sampaio, Alexandre P Zavascki, Afonso L Barth
No abstract text is available yet for this article.
March 7, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28263596/inactivation-and-gene-expression-of-a-virulent-wastewater-escherichia-coli-strain-and-the-nonvirulent-commensal-escherichia-coli-dsm1103-strain-upon-solar-irradiation
#12
Nada Al-Jassim, David Mantilla-Calderon, Tiannyu Wang, Pei-Ying Hong
This study examined the decay kinetics and molecular responses of two Escherichia coli strains upon solar irradiation. The first is E. coli PI-7, a virulent and antibiotic-resistant strain that was isolated from wastewater and carries the emerging NDM-1 antibiotic resistance gene. The other strain, E. coli DSM1103, displayed lower virulence and antibiotic resistance than E. coli PI-7. In a buffer solution, E. coli PI-7 displayed a longer lag phase prior to decay and a longer half-life compared with E. coli DSM1103 (6...
March 17, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28249575/first-report-on-bla-ndm-1-producing-acinetobacter-baumannii-in-three-clinical-isolates-from-ethiopia
#13
Michael Pritsch, Ahmed Zeynudin, Maxim Messerer, Simon Baumer, Gabriele Liegl, Soeren Schubert, Thomas Löscher, Michael Hoelscher, Tefara Belachew, Andrea Rachow, Andreas Wieser
BACKGROUND: Multidrug-resistant Gram-negative bacterial infections are recognized as one of the major threats to global health. In this study, we describe for the first time bla NDM-1 gene carrying organisms from Ethiopia consisting of three Acinetobacter baumannii isolates from patients in Jimma. METHODS: Besides phenotypic antimicrobial susceptibility testing, molecular strain typing and sequencing was performed to describe the phylogenetic relation of the Ethiopian isolates in detail in relation to published isolates from all over the globe...
March 1, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28242668/plasmid-mediated-novel-blandm-17-gene-encoding-a-carbapenemase-with-enhanced-activity-in-a-st48-escherichia-coli-strain
#14
Zhihai Liu, Yang Wang, Timothy R Walsh, Dejun Liu, Zhangqi Shen, Rongmin Zhang, Wenjuan Yin, Hong Yao, Jiyun Li, Jianzhong Shen
Carbapenem-resistant Enterobacteriaceae (CRE) have spread worldwide, leaving very few treatment options available. New Delhi metallo-beta-lactamase (NDM) is the main carbapenemase mediating CRE resistance, and is of increasing concern. NDM-positive Enterobacteriaceae of human origin are frequently identified; however, the emergence of NDM, particularly novel variants, in bacteria of food animal origin has never been reported. Here, we characterize a novel NDM variant (assigned NDM-17) identified in a β-lactam-resistant sequence type 48 (ST48) Escherichia coli strain that was isolated from a chicken in China...
February 27, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28210248/coexistence-of-blaoxa-48-and-truncated-blandm-1-on-different-plasmids-in-a-klebsiella-pneumoniae-isolate-in-china
#15
Lianyan Xie, Yi Dou, Kaixin Zhou, Yue Chen, Lizhong Han, Xiaokui Guo, Jingyong Sun
Objectives: To describe the genetic environment, transferability, and antibiotic susceptibility of one clinical Klebsiella pneumoniae isolate harboring both blaOXA-48 and blaNDM-1 on different plasmids from a Chinese hospital. Methods: The isolate was subjected to antimicrobial susceptibility testing and multilocus sequence typing using Etest and PCR. The plasmids harboring blaOXA-48 and blaNDM-1 were analyzed through conjugation experiments, S1-nuclease pulsed-field gel electrophoresis, and hybridization with specific probes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28197140/molecular-characteristics-of-carbapenem-resistant-enterobacter-cloacae-in-ningxia-province-china
#16
Zhiyun Shi, Huizheng Zhao, Gang Li, Wei Jia
The emergence of carbapenem-resistant Enterobacteriaceae (CRE) has become a major public health concern worldwide and a new challenge in the treatment of infectious diseases. The molecular characteristics of Enterobacter cloacae in Ningxia China are unknown. In this study, we reported 10 carbapenem-resistant E. cloacae isolates from the General Hospital of Ningxia Medical University, the largest university hospital in Ningxia between January 2012 and December 2013. Bacteria isolates were identified by Vitek2 compact and the identity of non-duplicate E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28191865/high-carbapenem-resistance-in-clinical-gram-negative-pathogens-isolated-in-egypt
#17
Hazim O Khalifa, Ahmed M Soliman, Ashraf M Ahmed, Toshi Shimamoto, Toshinori Hara, Mitsuyasu Ikeda, Yuta Kuroo, Shizuo Kayama, Motoyuki Sugai, Tadashi Shimamoto
The emergence and spread of carbapenem-resistant gram-negative bacteria poses a serious threat to human health worldwide. Currently, little is known about the molecular mechanisms underlying carbapenem resistance and their prevalence among gram-negative bacteria in Egypt. In this study, we analyzed carbapenemase production in gram-negative bacteria isolated from hospitalized patients in Egypt in 2014. All isolates were subjected to phenotypic and genotypic susceptibility testing for carbapenem resistance. Our results indicated a high level of carbapenem-resistant gram-negative bacteria in Egypt, with 50...
February 13, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28191309/co-existence-of-blaoxa-23-and-blandm-1-genes-of-acinetobacter-baumannii-isolated-from-nepal-antimicrobial-resistance-and-clinical-significance
#18
Prabhu Raj Joshi, Mahesh Acharya, Trishna Kakshapati, Udomluk Leungtongkam, Rapee Thummeepak, Sutthirat Sitthisak
BACKGROUND: Molecular analysis of carbapenem-resistant genes in Acinetobacter baumannii, an emerging pathogen, is less commonly reported from Nepal. In this study we determined the antibiotic susceptibility profile and genetic mechanism of carbapenem resistance in clinical isolates of A. baumannii. METHODS: A. baumannii were isolated from various clinical specimens and identified based on Gram staining, biochemical tests, and PCR amplification of organism specific 16S rRNA and blaOXA-51 genes...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28167564/multiclonal-expansion-of-klebsiella-pneumoniae-isolates-producing-ndm-1-in-rio-de-janeiro-brazil
#19
Caio Augusto Martins Aires, Polyana Silva Pereira, Carlos Felipe Machado de Araujo, Thiago Pavoni Gomes Chagas, Jane Cleide Ribeiro Oliveira, Sibelle Nogueira Buonora, Rodolpho Mattos Albano, Ana Paula D'Alincourt Carvalho-Assef, Marise Dutra Asensi
We characterized NDM-1-producing Klebsiella isolates from Rio de Janeiro, Brazil. PCR was applied for resistance and virulence determinants. The genetic context of blaNDM was determined by S1 nuclease pulsed-field gel electrophoresis (PFGE) and hybridization. Genotyping was performed by PFGE and multilocus sequence typing (MLST). Most isolates carried multiple resistance genes and remained susceptible to amikacin, fosfomycin-trometamol, polymyxin B, and tigecycline. The spread of NDM-1-producing Klebsiella pneumoniae was not associated with clonal expansion and appears to be associated with Tn3000...
April 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28167547/multicenter-clinical-and-molecular-epidemiological-analysis-of-bacteremia-due-to-carbapenem-resistant-enterobacteriaceae-cre-in-the-cre-epicenter-of-the-united-states
#20
Michael J Satlin, Liang Chen, Gopi Patel, Angela Gomez-Simmonds, Gregory Weston, Angela C Kim, Susan K Seo, Marnie E Rosenthal, Steven J Sperber, Stephen G Jenkins, Camille L Hamula, Anne-Catrin Uhlemann, Michael H Levi, Bettina C Fries, Yi-Wei Tang, Stefan Juretschko, Albert D Rojtman, Tao Hong, Barun Mathema, Michael R Jacobs, Thomas J Walsh, Robert A Bonomo, Barry N Kreiswirth
Although the New York/New Jersey (NY/NJ) area is an epicenter for carbapenem-resistant Enterobacteriaceae (CRE), there are few multicenter studies of CRE from this region. We characterized patients with CRE bacteremia in 2013 at eight NY/NJ medical centers and determined the prevalence of carbapenem resistance among Enterobacteriaceae bloodstream isolates and CRE resistance mechanisms, genetic backgrounds, capsular types (cps), and antimicrobial susceptibilities. Of 121 patients with CRE bacteremia, 50% had cancer or had undergone transplantation...
April 2017: Antimicrobial Agents and Chemotherapy
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