keyword
https://read.qxmd.com/read/34728097/biomolecular-evaluation-of-cryopreserved-amniotic-membranes-for-ophthalmological-use-by-elisa-and-rt-pcr-at-one-and-eighteen-months
#21
JOURNAL ARTICLE
C Wahab, O Fakhoury, H Serhan, J Ayash, F Jabbour, A Dirani, M Kallassy, N Waked
PURPOSE: To study the presence of certain proteins - EGF (epidermal growth factor), KGF (keratinocyte growth factor), IL-10 (interleukin 10), HGF (hepatocyte growth factor), Alpha2-macroglobulin and IL-1RA (interleukin 1 receptor antagonist) in cryopreserved amniotic membranes at 1 and 18 months and, as a secondary objective, to detect mRNA corresponding to KGF, IL-1Ra, Alpha2-macroglobulin, Fas Ligand, TGF beta (transforming growth factor beta) and Lumican by RT-PCR in membranes preserved at 1 and 18 months...
December 2021: Journal Français D'ophtalmologie
https://read.qxmd.com/read/33839725/vascular-patterning-as-integrative-readout-of-complex-molecular-and-physiological-signaling-by-vessel-generation-analysis
#22
REVIEW
Mark Lagatuz, Ruchi J Vyas, Marina Predovic, Shiyin Lim, Nicole Jacobs, Miguel Martinho, Hamed Valizadegan, David Kao, Nikunj Oza, Corey A Theriot, Susana B Zanello, Giovanni Taibbi, Gianmarco Vizzeri, Mariana Dupont, Maria B Grant, Daniel J Lindner, Hans-Christian Reinecker, Alexander Pinhas, Toco Y Chui, Richard B Rosen, Nicanor Moldovan, Mary B Vickerman, Krishnan Radhakrishnan, Patricia Parsons-Wingerter
The molecular signaling cascades that regulate angiogenesis and microvascular remodeling are fundamental to normal development, healthy physiology, and pathologies such as inflammation and cancer. Yet quantifying such complex, fractally branching vascular patterns remains difficult. We review application of NASA's globally available, freely downloadable VESsel GENeration (VESGEN) Analysis software to numerous examples of 2D vascular trees, networks, and tree-network composites. Upon input of a binary vascular image, automated output includes informative vascular maps and quantification of parameters such as tortuosity, fractal dimension, vessel diameter, area, length, number, and branch point...
2021: Journal of Vascular Research
https://read.qxmd.com/read/33825855/interleukin-1-and-transforming-growth-factor-beta-commonly-opposing-but-sometimes-supporting-master-regulators-of-the-corneal-wound-healing-response-to-injury
#23
JOURNAL ARTICLE
Steven E Wilson
Purpose: Interleukin (IL)-1α/IL-1β and transforming growth factor (TGF)β1/TGFβ2 have both been promoted as "master regulators" of the corneal wound healing response due to the large number of processes each regulates after injury or infection. The purpose of this review is to highlight the interactions between these systems in regulating corneal wound healing. Methods: We conducted a systematic review of the literature. Results: Both regulator pairs bind to receptors expressed on keratocytes, corneal fibroblasts, and myofibroblasts, as well as bone marrow-derived cells that include fibrocytes...
April 1, 2021: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/33808279/the-metabolite-profile-in-culture-supernatant-of-aster-yomena-callus-and-its-anti-photoaging-effect-in-skin-cells-exposed-to-uvb
#24
JOURNAL ARTICLE
Woo Sik Kim, Jeong Hun Seo, Jae-In Lee, Eun-Sil Ko, Sang-Min Cho, Jea-Ran Kang, Jong-Hoon Jeong, Yu Jeong Jeong, Cha Young Kim, Jeong-Dan Cha, Young-Bae Ryu
Aster yomena ( A. yomena ) extract has anti-inflammatory, antioxidant, anti-asthma, and anti-atopic effects. However, the commercial use of A. yomena extract requires a long processing time with specific processing steps (including heat treatment and ethanol precipitation), and there are various environmental problems. We aimed to build a system to produce A. yomena extract by culturing the callus in a bioreactor that can allow rapid process scale-up to test the effect of extract (AYC-CS-E) isolated from culture supernatant of A...
March 30, 2021: Plants (Basel, Switzerland)
https://read.qxmd.com/read/33642112/possible-treatment-for-uvb-induced-skin-injury-anti-inflammatory-and-cytoprotective-role-of-metformin-in-uvb-irradiated-keratinocytes
#25
JOURNAL ARTICLE
Ta Xiao, Yujie Chen, Changjun Song, Song Xu, Shangqing Lin, Min Li, Xu Chen, Heng Gu
BACKGROUND: Excessive inflammation and cell death induced by ultraviolet (UV) cause skin photodamage. Metformin possesses anti-inflammatory and cytoprotective effects. However, whether metformin inhibits inflammation and cell death in UVB-induced acute skin damage is unclear. OBJECTIVE: To evaluate the anti-inflammatory and cytoprotective effects of metformin in vitro and in vivo. Furthermore, its potential mechanism has been explored. METHODS: Transcriptome sequencing and multiplex cytokines analysis were used to evaluate the validity of in vitro UVB-induced acute damage keratinocyte model and anti-inflammatory effects of metformin...
April 2021: Journal of Dermatological Science
https://read.qxmd.com/read/33596041/strategy-for-treatment-of-infected-diabetic-foot-ulcers
#26
REVIEW
Mayland Chang, Trung T Nguyen
Diabetic foot ulcers (DFUs) are chronic wounds that develop in 30% of diabetic patients. In DFUs, the normal wound healing process consisting of inflammation, angiogenesis, and extracellular matrix (ECM) remodeling is dysregulated and stalled. Upon injury, neutrophils and monocytes arrive at the wound and secrete matrix metalloproteinase (MMP)-8 and reactive oxygen species (ROS). ROS activates nuclear factor kappa beta (NF-κB), which upregulates MMP-9. Monocytes become macrophages, secreting tumor growth factor (TGF)-β1 and vascular endothelial growth factor (VEGF) for angiogenesis, resulting in remodeling of the ECM...
March 2, 2021: Accounts of Chemical Research
https://read.qxmd.com/read/33511059/evaluation-of-wound-healing-activity-of-ethanol-extract-of-annona-reticulata-l-leaf-both-in-vitro-and-in-diabetic-mice-model
#27
JOURNAL ARTICLE
Swagata Mazumdar, Amit K Ghosh, Manikarna Dinda, Anjan K Das, Saurabh Das, Kuladip Jana, Parimal Karmakar
Background and aim: The leaves of Annonareticulata Linn (niú x ınguǒ; Bullock's heart), a member of Annonaceae family, have been used extensively in folk medicine; however, its wound healing potential is yet to be explored. Our aim was to investigate the wound healing ability of A. reticulata leaf extract in vitro and in streptozotocin induced diabetic mice model. Material and methods: We observed the plant extract induced proliferation and migration of primary human dermal fibroblast (HDF), human skin fibroblast cell line (GM00637) and human keratinocyte cell line (HACAT)...
January 2021: Journal of Traditional and Complementary Medicine
https://read.qxmd.com/read/33422138/human-foreskin-derived-dermal-stem-progenitor-cell-conditioned-medium-combined-with-hyaluronic-acid-promotes-extracellular-matrix-regeneration-in-diabetic-wounds
#28
JOURNAL ARTICLE
Yu Xin, Peng Xu, Xiangsheng Wang, Yunsheng Chen, Zheng Zhang, Yixin Zhang
BACKGROUND: Diabetic wounds remain a challenging clinical problem, which requires further treatment development. Published data showed that dermis-derived stem/progenitor cells (DSPCs) display superior wound healing in vitro. The beneficial effects of DSPCs are mediated through paracrine secretion, which can be obtained from conditioned medium (CM). Hyaluronic acid (HA) is especially suitable for skin regeneration and delivering bioactive molecules in CM. This study investigated the effect of human foreskin-derived dermal stem/progenitor cell (hFDSPC)-CM combined with HA on a diabetic mouse model and relevant mechanism in vitro...
January 9, 2021: Stem Cell Research & Therapy
https://read.qxmd.com/read/33336085/phospholipase-c-epsilon-mediates-cytokine-cascade-induced-by-acute-disruption-of-epidermal-permeability-barrier-in-mice
#29
JOURNAL ARTICLE
Jing Zhang, Jiangmei Wu, Mengke Sun, Shuchang Zhang, Junkai Huang, Maoqiang Man, Lizhi Hu
Disruption of epidermal barrier is an important trigger in abnormal cutaneous inflammation. Phospholipase C epsilon (PLCε), a Ras/Rap1 effector, is essential for regulating cytokines production in different types of skin inflammation. Our previous studies have demonstrated that elevated expression of PLCε participates in the psoriasis-like inflammation in PLCε overexpressing transgenic mice model, while the reduction in PLCε expression attenuates inflammatory responses in either TPA- or DNFB-induced cutaneous inflammation...
December 2020: Biochemistry and Biophysics Reports
https://read.qxmd.com/read/33262631/tgf-%C3%AE-1-gene-polymorphism-and-its-correlation-with-serum-level-of-tgf-%C3%AE-1-in-psoriasis-vulgaris-among-iraqi-people
#30
JOURNAL ARTICLE
Bryar T Ahmed, Mohammad Y Saeed, Saman H Noori, Dashty M Amin
PURPOSE: Many cytokines have been implicated in the pathogenesis of psoriasis, among these the transforming growth factor-beta 1 (TGF-β1) can be endorsed by different mechanisms besides inhibiting keratinocytes proliferation. The role of genetic polymorphisms of TGF-β1 has been studied in various inflammatory diseases. Our aim is to study the correlation of TGF-β1 gene polymorphism at codon 10 and 25 with the expression of serum level of TGF-β1 in a sample of Iraqi psoriatic patients compared to the control group...
2020: Clinical, Cosmetic and Investigational Dermatology
https://read.qxmd.com/read/32813021/tgf-%C3%AE-1-modulates-temozolomide-resistance-in-glioblastoma-via-altered-microrna-processing-and-elevated-mgmt
#31
JOURNAL ARTICLE
Er Nie, Xin Jin, Faan Miao, Tianfu Yu, Tongle Zhi, Zhumei Shi, Yingyi Wang, Junxia Zhang, Manyi Xie, Yongping You
BACKGROUND: Our previous studies have indicated that miR-198 reduces cellular methylguanine DNA methyltransferase (MGMT) levels to enhance temozolomide sensitivity. Transforming growth factor beta 1 (TGF-β1) switches off miR-198 expression by repressing K-homology splicing regulatory protein (KSRP) expression in epidermal keratinocytes. However, the underlying role of TGF-β1 in temozolomide resistance has remained unknown. METHODS: The distribution of KSRP was detected by western blotting and immunofluorescence...
March 25, 2021: Neuro-oncology
https://read.qxmd.com/read/32671095/differential-wound-healing-capacity-of-mesenchymal-stem-cell-derived-exosomes-originated-from-bone-marrow-adipose-tissue-and-umbilical-cord-under-serum-and-xeno-free-condition
#32
JOURNAL ARTICLE
Diem Huong Hoang, Tu Dac Nguyen, Hoang-Phuong Nguyen, Xuan-Hung Nguyen, Phuong Thi Xuan Do, Van Duc Dang, Phuong Thi Minh Dam, Hue Thi Hong Bui, Mai Quynh Trinh, Duc Minh Vu, Nhung Thi My Hoang, Liem Nguyen Thanh, Uyen Thi Trang Than
Exosomes are nano-scale and closed membrane vesicles which are promising for therapeutic applications due to exosome-enclosed therapeutic molecules such as DNA, small RNAs, proteins and lipids. Recently, it has been demonstrated that mesenchymal stem cell (MSC)-derived exosomes have capacity to regulate many biological events associated with wound healing process, such as cell proliferation, cell migration and blood vessel formation. This study investigated the regenerative potentials for cutaneous tissue, in regard to growth factors associated with wound healing and skin cell proliferation and migration, by exosomes released from primary MSCs originated from bone marrow (BM), adipose tissue (AD), and umbilical cord (UC) under serum- and xeno-free condition...
2020: Frontiers in Molecular Biosciences
https://read.qxmd.com/read/32397594/cellular-response-to-vitamin-c-enriched-chitosan-agarose-film-with-potential-application-as-artificial-skin-substitute-for-chronic-wound-treatment
#33
JOURNAL ARTICLE
Vladyslav Vivcharenko, Michal Wojcik, Agata Przekora
The treatment of chronic wounds is still a meaningful challenge to physicians. The aim of this work was to produce vitamin C-enriched chitosan/agarose (CHN/A) film that could serve as potential artificial skin substitute for chronic wound treatment. The biomaterial was fabricated by a newly developed and simplified method via mixing acidic chitosan solution with alkaline agarose solution that allowed to obtain slightly acidic pH (5.97) of the resultant material, which is known to support skin regeneration. Vitamin C was immobilized within the matrix of the film by entrapment method during production process...
May 10, 2020: Cells
https://read.qxmd.com/read/32384749/effect-of-kelulut-honey-on-the-cellular-dynamics-of-tgf%C3%AE-induced-epithelial-to-mesenchymal-transition-in-primary-human-keratinocytes
#34
JOURNAL ARTICLE
Abid Nordin, Shiplu Roy Chowdhury, Aminuddin Bin Saim, Ruszymah Bt Hj Idrus
Over-induction of epithelial to mesenchymal transition (EMT) by tumor growth factor beta (TGFβ) in keratinocytes is a key feature in keloid scar. The present work seeks to investigate the effect of Kelulut honey (KH) on TGFβ-induced EMT in human primary keratinocytes. Image analysis of the real time observation of TGFβ-induced keratinocytes revealed a faster wound closure and individual migration velocity compared to the untreated control. TGFβ-induced keratinocytes also have reduced circularity and display a classic EMT protein expression...
May 6, 2020: International Journal of Environmental Research and Public Health
https://read.qxmd.com/read/32330363/wound-healing-action-of-nitric-oxide-releasing-self-expandable-collagen-sponge
#35
JOURNAL ARTICLE
Valéria C O Póvoa, Giovanna J V P Dos Santos, Guilherme F Picheth, Carlos P Jara, Laura C E da Silva, Eliana P de Araújo, Marcelo G de Oliveira
Mounting evidence showing that local nitric oxide (NO) delivery may significantly improve the wound healing process has stimulated the development of wound dressings capable of releasing NO topically. Herein, we describe the preparation of a self-expandable NO-releasing hydrolyzed collagen sponge (CS), charged with the endogenously found NO donor, S-nitrosoglutathione (GSNO). We show that cold pressed and GSNO-charged CS (CS/GSNO) undergo self-expansion to its original 3D shape upon water absorption to a swelling degree of 2300 wt%, triggering the release of free NO...
April 24, 2020: Journal of Tissue Engineering and Regenerative Medicine
https://read.qxmd.com/read/32188026/identification-of-deregulated-pathways-key-regulators-and-novel-mirna-mrna-interactions-in-hpv-mediated-transformation
#36
JOURNAL ARTICLE
Iris Babion, Viktorian Miok, Annelieke Jaspers, Angelina Huseinovic, Renske D M Steenbergen, Wessel N van Wieringen, Saskia M Wilting
Next to a persistent infection with high-risk human papillomavirus (HPV), molecular changes are required for the development of cervical cancer. To identify which molecular alterations drive carcinogenesis, we performed a comprehensive and longitudinal molecular characterization of HPV-transformed keratinocyte cell lines. Comparative genomic hybridization, mRNA, and miRNA expression analysis of four HPV-containing keratinocyte cell lines at eight different time points was performed. Data was analyzed using unsupervised hierarchical clustering, integrated longitudinal expression analysis, and pathway enrichment analysis...
March 16, 2020: Cancers
https://read.qxmd.com/read/32015752/skin-hydration-effects-of-scale-up-fermented-cyclopia-intermedia-against-ultraviolet-b-induced-damage-in-keratinocyte-cells-and-hairless-mice
#37
JOURNAL ARTICLE
A-Rang Im, Sung Hum Yeon, Kon-Young Ji, Rak Ho Son, Key An Um, Sungwook Chae
Photoaging occurs by chronic skin exposure to the sun and ultraviolet irradiation and leads to skin aging accompanied by a lack of skin hydration. We previously demonstrated the photoprotective effect of fermented Cyclopia intermedia (honeybush) extract on the skin. In this study, we evaluated the skin hydration effects of scaled-up fermented honeybush extract (HU-018) against ultraviolet B (UVB) radiation in HaCaT immortalized human keratinocytes and hairless mice. Pretreating HaCaT cells with HU-018 attenuated the decreased hyaluronic acid (HA) levels and mRNA expression of genes encoding involucrin, filaggrin, and loricrin by UVB irradiation...
2020: Evidence-based Complementary and Alternative Medicine: ECAM
https://read.qxmd.com/read/31953581/alda-1-an-aldehyde-dehydrogenase-2-agonist-improves-cutaneous-wound-healing-by-activating-epidermal-keratinocytes-via-akt-gsk-3%C3%AE-%C3%AE-catenin-pathway
#38
JOURNAL ARTICLE
Simin Zhang, Cheng Chen, Jianghui Ying, Chuanyuan Wei, Lu Wang, Zhen Yang, Fazhi Qi
BACKGROUND: The cutaneous wound healing process mainly comprises re-epithelialization, fibrosis, and neovascularization. Impaired wound healing is common but tricky in plastic surgery. Aldehyde dehydrogenase 2 (ALDH2), the most effective subset of the ALDH enzyme family, is known to exert a major role in detoxification of aldehydes. Activation of ALDH2 by Alda-1 (a specific agonist) has been found to protect against cardiovascular diseases. However, no research has paid attention to the potential of ALDH2 activation in regulating wound healing...
January 17, 2020: Aesthetic Plastic Surgery
https://read.qxmd.com/read/31930527/lichen-planus-related-to-transforming-growth-factor-beta-inhibitor-in-a-patient-with-metastatic-chondrosarcoma-a-case-report
#39
Maya Farah, Kelly C Nelson, Michael T Tetzlaff, Priyadharsini Nagarajan, Carlos A Torres-Cabala, Victor G Prieto, Jonathan L Curry, Phyu P Aung
Transforming growth factor-beta1 (TGF-β1) is expressed in normal epidermis. TGF-β1 potently inhibits keratinocyte proliferation and immunomodulatory properties, mainly by suppressing immune responses to self-antigens. Lichen planus (LP) is a form of dermatitis caused by cell-mediated immune dysfunction, but the exact pathogenic pathways are unknown, which poses therapeutic challenges. We report on a 68-year-old man who developed multiple pruritic, discrete, and well-demarcated, flat-topped red-purple papules and macules on the back and upper arms following 4 cycles of treatment with TGF-β receptor I (TGFBR-I) inhibitor, ly3200882, for metastatic chondrosarcoma...
May 2020: Journal of Cutaneous Pathology
https://read.qxmd.com/read/31825120/ripk4-suppresses-the-tgf-%C3%AE-1-signaling-pathway-in-hacat-cells
#40
JOURNAL ARTICLE
Tuba Dinçer, Asiye Büşra Boz Er, İdris Er, Bayram Toraman, Gokhan Yildiz, Ersan Kalay
Receptor-interacting serine/threonine kinase 4 (RIPK4) and transforming growth factor-beta 1 (TGF-β1) play critical roles in the development and maintenance of the epidermis. A negative correlation between the expression patterns of RIPK4 and TGF-β signaling during epidermal homeostasis related events and suppression of RIPK4 expression by TGF-β1 in keratinocyte cell lines suggest the presence of a negative regulatory loop between two factors. So far, RIPK4 has been shown to regulate nuclear factor-κB (NF-κB), protein kinase C (PKC), wingless-type MMTV integration site family (Wnt), and (mitogen-activated protein kinase) MAPK signaling pathways...
December 11, 2019: Cell Biology International
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