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hi-c OR hic

Y Luo, Y Y Huang, Y Jin, C R Li, J Yang
Objective: To investigate the effect of microRNAs (miR)-21 on the expression of interleukin (IL)-10 in B cell of patients with Henoch-Schonlein purpura (HSP). Methods: From March 2016 to January 2017, twenty-four children with HSP hospitalized in rheumatology and immunology department of Shenzhen Children's Hospital were enrolled into the study, including 12 males and 12 females. Patients were divided into purpura nephritis group (HSPN, 14 cases) and non-nephritis group (NHSPN, 10 cases). The age-matched 34 healthy children were included as the control group for prospective cohort study...
December 2, 2018: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
Ivan D Florez, Areti-Angeliki Veroniki, Reem Al Khalifah, Juan J Yepes-Nuñez, Javier M Sierra, Robin W M Vernooij, Jorge Acosta-Reyes, Claudia M Granados, Giordano Pérez-Gaxiola, Carlos Cuello-Garcia, Adriana M Zea, Yuan Zhang, Naghmeh Foroutan, Gordon H Guyatt, Lehana Thabane
BACKGROUND: Many interventions have shown effectiveness in reducing the duration of acute diarrhea and gastroenteritis (ADG) in children. Yet, there is lack of comparative efficacy of interventions that seem to be better than placebo among which, the clinicians must choose. Our aim was to determine the comparative effectiveness and safety of the pharmacological and nutritional interventions for reducing the duration of ADG in children. METHODS: Data sources included Medline, Embase, CENTRAL, CINAHL, LILACS, and Global-Health up to May 2017...
2018: PloS One
Yanyun He, Ce Shang, Frank J Chaloupka
OBJECTIVES: This study calculates cigarette affordability for 78 countries worldwide from 2001 to 2014 using the Relative Income Price (RIP) ratio defined as the percentage of per capita GDP required to purchase 100 packs of cigarettes using the lowest price from Economist Intelligence Unit (EIU) database, examine the association between cigarette affordability and cigarette consumption, and calculate the affordability elasticity of demand. DESIGN AND METHODS: RIP (2001-2014) was calculated for 16 low-income economies, 19 lower middle-income economies, 13 upper middle-income economies, and 30 high-income economies...
2018: PloS One
Shicheng Yu, Chaoran Zheng, Fan Zhou, David L Baillie, Ann M Rose, Zixin Deng, Jeffrey Shih-Chieh Chu
BACKGROUND: Essential genes are required for an organism's viability and their functions can vary greatly, spreading across many pathways. Due to the importance of essential genes, large scale efforts have been undertaken to identify the complete set of essential genes and to understand their function. Studies of genome architecture and organization have found that genes are not randomly disturbed in the genome. RESULTS: Using combined genetic mapping, Illumina sequencing, and bioinformatics analyses, we successfully identified 44 essential genes with 130 lethal mutations in genomic regions of C...
December 4, 2018: BMC Genomics
Michelle A Nipper, Jeremiah P Jensen, Melinda L Helms, Matthew M Ford, John C Crabbe, David J Rossi, Deborah A Finn
Sensitivity to anticonvulsant effects of the γ-aminobutyric acidA receptor-active neurosteroid allopregnanolone (ALLO) during ethanol withdrawal varies across genotypes, with high sensitivity in genotypes with mild withdrawal and low sensitivity in genotypes with high withdrawal. The present studies determined whether the resistance to ALLO during withdrawal in mouse genotypes with high handling-induced convulsions (HICs) during withdrawal could be overcome with use of ganaxolone (GAN), the metabolically stable derivative of ALLO...
December 1, 2018: Neuroscience
Marco Canevelli, Valerio Zaccaria, Ciro Ruocco, Martina Valletta, Marina Gasparini, Nicola Vanacore, Matteo Cesari, Giuseppe Bruno
INTRODUCTION: The phenomenon of dementia occurring in migrants and minority groups constitutes an emerging issue for Western countries. Nevertheless, it has been poorly explored from the perspective of "real-world" clinical services. We aimed to quantify the number of migrants from LMIC attending an Italian university memory clinic and to document its modifications over time. METHODS: All the subjects undergoing a first neurological and cognitive assessment between 2001 and 2017 were considered for the present analyses...
December 4, 2018: Neurological Sciences
Lei Liu, Guang Shi, D Thirumalai, Changbong Hyeon
We investigate spatiotemporal dynamics of human interphase chromosomes by employing a heteropolymer model that incorporates the information of human chromosomes inferred from Hi-C data. Despite considerable heterogeneities in the chromosome structures generated from our model, chromatins are organized into crumpled globules with space-filling (SF) statistics characterized by a single universal scaling exponent (ν = 1/3), and this exponent alone can offer a quantitative account of experimentally observed, many different features of chromosome dynamics...
December 3, 2018: PLoS Computational Biology
Mingyan Cao, Sri Hari Raju Mulagapati, Bhargavi Vemulapalli, Jihong Wang, Sergei V Saveliev, Marjeta Urh, Alan Hunter, Dengfeng Liu
Characterization of asparagine deamidation and aspartic acid isomerization is an important aspect of biotherapeutic protein analysis due to the potential negative effect of these modifications on drug efficacy and stability. Succinimide has long been known to be an intermediate product of asparagine deamidation and aspartic acid isomerization, but despite the key role of succinimide in these reactions, its analysis remains challenging due to its instability. We have developed a paradigm in which two interlinked analytical methods are used to develop an optimized approach to analyze succinimide...
November 29, 2018: Analytical Biochemistry
Carol M House, Michael J Tipton, Philip M Hopkins, Daniel Roiz de Sa
OBJECTIVES: The study was undertaken to compare the thermal and biochemical responses to a heat tolerance test (HTT) of malignant hyperthermia (MH) susceptible individuals, volunteers who have suffered heat illness (HI) and control volunteers. METHODS: Three groups of male volunteers (n=6 in each group) were recruited to the study: MHS - civilian volunteers previously diagnosed as MH susceptible; EHI - military volunteers with a history of exertional HI; CON - military volunteers with no history of HI or MH...
November 23, 2018: Journal of Science and Medicine in Sport
Naomi J Wright, Niyi Ade-Ajayi, Kokila Lakhoo
This article describes the Global Surgery Symposium held within the 65th British Association of Paediatric Surgeons (BAPS) Annual Congress in 2018. Global surgery is a rapidly expanding and developing field and is of particular importance in paediatrics since children account for up to 50% of the population in low- and middle-income countries (LMICs). It is estimated that up to a third of childhood deaths in LMICs are the result of a surgical condition, and congenital anomalies have risen to become the 5th leading cause of death in children less than 5-years of age globally...
November 7, 2018: Journal of Pediatric Surgery
Lei Wang, R Kenneth Marcus
Poly(ethylene terephthalate) (polyester, PET) capillary-channeled polymer fibers are used as the stationary phase for the separation of a synthetic protein mixtures composed of cytochrome c, myoglobin, ribonuclease A, lysozyme, α-chymotrypsinogen A, α-chymotrypsin and holo-transferrin. Polyester is a useful phase for HIC, having a mixed alkyl and aromatic backbone to yield mildly hydrophobic properties, an aromatic functionality allowing for π-π interactions, and carboxylate end groups adding hydrophilicity...
November 26, 2018: Journal of Chromatography. A
C P Philbrick, J Westerhoff, Z Wang, S Hall, G R Swenson
The photometer payload of the lower atmosphere ionosphere coupling experiment CubeSat mission will observe and characterize atmospheric gravity wave (AGW) propagation through the mesosphere/lower thermosphere region of Earth's atmosphere on a global scale. AGW characteristics will be measured via passive observation of airglow emission from atmospheric O2 ( <mml:math xmlns:mml=""> <mml:msup> <mml:mrow> <mml:mi>b</mml:mi> </mml:mrow> <mml:mrow> <mml:mn>1</mml:mn> </mml:mrow> </mml:msup> <mml:msubsup> <mml:mrow> <mml:mi>Σ</mml:mi> </mml:mrow> <mml:mrow> <mml:mi>g</mml:mi> </mml:mrow> <mml:mrow> <mml:mo>+</mml:mo> </mml:mrow> </mml:msubsup> </mml:math> ) at 762...
November 2018: Review of Scientific Instruments
Joost de Graaf, Wilson C K Poon, Magnus J Haughey, Michiel Hermes
Colloidal particles with strong, short-ranged attractions can form a gel. We simulate this process without and with hydrodynamic interactions (HI), using the lattice-Boltzmann method to account for presence of a thermalized solvent. We show that HI speed up and slow down gelation at low and high volume fractions, respectively. The transition between these two regimes is linked to the existence of a percolating cluster shortly after quenching the system. However, when we compare gels at matched 'structural age', we find nearly indistinguishable structures with and without HI...
November 30, 2018: Soft Matter
Melissa Latigo Mugambi, Trevor Peter, Samuel F Martins, Cristina Giachetti
Diagnostics developers often face challenges introducing in-vitro diagnostic (IVD) products to low- and middle-income countries (LMICs) because of difficulty in accessing robust market data, navigating policy and regulatory requirements and implementing and supporting products in healthcare systems with limited infrastructure. Best practices recommend the use of a phase-gate model with defined activities and milestones by phase to successfully move a product from concept to commercialisation. While activities for commercialisation of products in high-income countries (HICs) are well understood, the activities required for introduction of IVDs in LMICs are not...
2018: BMJ Global Health
Noelia Díaz, Kai Kruse, Tabea Erdmann, Annette M Staiger, German Ott, Georg Lenz, Juan M Vaquerizas
Chromatin conformation constitutes a fundamental level of eukaryotic genome regulation. However, our ability to examine its biological function and role in disease is limited by the large amounts of starting material required to perform current experimental approaches. Here, we present Low-C, a Hi-C method for low amounts of input material. By systematically comparing Hi-C libraries made with decreasing amounts of starting material we show that Low-C is highly reproducible and robust to experimental noise. To demonstrate the suitability of Low-C to analyse rare cell populations, we produce Low-C maps from primary B-cells of a diffuse large B-cell lymphoma patient...
November 29, 2018: Nature Communications
Usha Kumari, Sushanta K Behera, B C Meikap
Excessive fluoride contamination in ground and surface water is hazardous to human health. Adsorptive removal is a better option for defluoridation due to its simplicity and efficient working property. In the current research, an attempt was made for the removal of fluoride ions from wastewater by a novel adsorbent synthesized with alumina and H2 SO4 acid by acidic activation. The adsorbent was characterized for physio-chemical properties by several analytical methods (SEM, EDX, FTIR, XRF, TGA, XRD, HI and pHZPC )...
November 16, 2018: Journal of Hazardous Materials
L E Durán-Carabali, D M Arcego, E F Sanches, F K Odorcyk, R M Marques, A Tosta, L Reichert, S A Carvalho, C Dalmaz, C A Netto
Environmental enrichment (EE) at early stages of neurodevelopment attenuates HI-induced behavioral, histological and cellular damage. However, the effects of EE exposure during gestational or early postnatal period and the possible influence of sexual dimorphism on EE protection are not fully understood. Present study evaluated the effects of pre-natal and postnatal EE, as well as their combination, in male and female rats submitted to neonatal HI at postnatal day (PND) 3. Wistar rats were housed in EE or in standard condition (SC) during all pregnancy...
November 26, 2018: Behavioural Brain Research
Ioannis Hodges-Mameletzis, Shona Dalal, Busisiwe Msimanga-Radebe, Michelle Rodolph, Rachel Baggaley
In September 2015, the World Health Organization (WHO) launched evidence-based guidelines by recommending that any person at substantial HIV risk should be offered oral pre-exposure prophylaxis (PrEP) containing tenofovir disoproxil fumarate (TDF) as an additional prevention choice. Since 2017, PrEP medicines have also been listed in the WHO's Essential Medicines List, including TDF/emtricitabine (FTC) and TDF in combination with lamivudine (3TC). A descriptive policy review and analysis of countries adopting WHO's 2015 recommendation on oral PrEP was conducted...
November 2018: Sexual Health
Ryan K Shultzaberger, Rachel E Abrams, Challise J Sullivan, Anthony D Schmitt, Thomas W J Thompson, John Dresios
There is an established relationship between primary DNA sequence, secondary and tertiary chromatin structure, and transcriptional activity, suggesting that observed differences in one of these properties may reflect changes in the others. Here, we exploit these relationships to show that variations in DNA structure can be used to identify a wide range of genomic alterations in mammalian samples. In this proof-of-concept study we characterized and compared genome-wide histone occupancy by ChIP-Seq, DNA accessibility by ATAC-Seq, and chromosomal conformation by Hi-C for five CRISPR/Cas9-modified mammalian cell lines and their unmodified parent strains, as well as in one modified tissue sample and its parent strain...
2018: PloS One
Mehmet Akgul, Alper Ozgurluk, Clark T-C Nguyen
This part II of a two-paper sequence presents fabri-cation and measurement results for a micromechanical disk-based RF channel-select filter designed using the theory and procedure of Part I. Successful demonstration of an actual filter required sev-eral practical additions to an ideal design, including the introduc-tion of a 39nm-gap capacitive transducer, voltage-controlled fre-quency tuning electrodes, and a stress relieving coupled array de-sign, all of which combine to enable a 0.1%-bandwidth 223.4-MHz channel-select filter with only 2...
November 26, 2018: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
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