keyword
https://read.qxmd.com/read/38501835/ace-2-like-enzymatic-activity-is-associated-with-immunoglobulin-in-covid-19-patients
#1
JOURNAL ARTICLE
Yufeng Song, Regan Myers, Frances Mehl, Lila Murphy, Bailey Brooks, Jeffrey M Wilson, Alexandra Kadl, Judith Woodfolk, Steven L Zeichner
UNLABELLED: Many mechanisms responsible for COVID-19 pathogenesis are well-established, but COVID-19 includes features with unclear pathogenesis, such as autonomic dysregulation, coagulopathies, and high levels of inflammation. The receptor for the SARS-CoV-2 spike protein receptor-binding domain (RBD) is angiotensin-converting enzyme 2 (ACE2). We hypothesized that some COVID-19 patients may develop antibodies that have a negative molecular image of RBD sufficiently similar to ACE2 to yield ACE2-like catalytic activity-ACE2-like abzymes...
March 19, 2024: MBio
https://read.qxmd.com/read/38132463/hydrolysis-of-oligodeoxyribonucleotides-on-the-microarray-surface-and-in-solution-by-catalytic-anti-dna-antibodies-in-systemic-lupus-erythematosus
#2
JOURNAL ARTICLE
Tatiana S Novikova, Evgeny A Ermakov, Elena V Kostina, Alexander N Sinyakov, Alexey E Sizikov, Georgy A Nevinsky, Valentina N Buneva
Anti-DNA antibodies are known to be classical serological hallmarks of systemic lupus erythematosus (SLE). In addition to high-affinity antibodies, the autoantibody pool also contains natural catalytic anti-DNA antibodies that recognize and hydrolyze DNA. However, the specificity of such antibodies is uncertain. In addition, DNA binding to a surface such as the cell membrane, can also affect its recognition by antibodies. Here, we analyzed the hydrolysis of short oligodeoxyribonucleotides (ODNs) immobilized on the microarray surface and in solution by catalytic anti-DNA antibodies from SLE patients...
December 8, 2023: Current Issues in Molecular Biology
https://read.qxmd.com/read/37431466/some-structural-features-of-the-peptide-profile-of-myelin-basic-protein-hydrolyzing-antibodies-in-schizophrenic-patients
#3
JOURNAL ARTICLE
Maria Zavialova, Daria Kamaeva, Laura Kazieva, Vladlen S Skvortsov, Liudmila Smirnova
The antibodies of schizophrenic patients that hydrolyze myelin basic protein (MBP) have been actively studied recently, but the mechanism of the catalytic properties of immunoglobulin molecules remains unknown. Determination of specific immunoglobulin sequences associated with the high activity of MBP proteolysis will help to understand the mechanisms of abzyme catalysis. In the course of comparative mass spectrometric analysis of IgG peptides from the blood serum of patients with acute schizophrenia and healthy people, 12 sequences were identified, which were found only in antibodies that hydrolyze MBP...
2023: PeerJ
https://read.qxmd.com/read/37239982/eae-of-mice-enzymatic-cross-site-specific-hydrolysis-of-h2a-histone-by-iggs-against-h2a-h1-h2b-h3-and-h4-histones-and-myelin-basic-protein
#4
JOURNAL ARTICLE
Andrey E Urusov, Kseniya S Aulova, Pavel S Dmitrenok, Valentina N Buneva, Georgy A Nevinsky
Histones play vital roles in chromatin function and gene transcription; however, they are very harmful in the intercellular space because they stimulate systemic inflammatory and toxic responses. Myelin basic protein (MBP) is the major protein of the axon myelin-proteolipid sheath. Antibodies-abzymes with various catalytic activities are specific features of some autoimmune diseases. IgGs against individual histones (H2A, H1, H2B, H3, and H4) and MBP were isolated from the blood of experimental-autoimmune-encephalomyelitis-prone C57BL/6 mice by several affinity chromatographies...
May 12, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37189796/the-influence-of-antipsychotic-treatment-on-the-activity-of-abzymes-targeting-myelin-and-levels-of-inflammation-markers-in-patients-with-schizophrenia
#5
JOURNAL ARTICLE
Daria A Kamaeva, Daria V Kazantseva, Anastasiia S Boiko, Irina A Mednova, Liudmila P Smirnova, Elena G Kornetova, Svetlana A Ivanova
Catalytic antibodies, or abzymes, are capable of not only binding but also hydrolyzing various proteins. Previously, an increase in the level of myelin basic protein (MBP)-hydrolyzing activity of antibodies was shown in patients with a number of neurological and mental disorders, including schizophrenia. Furthermore, antipsychotic therapy is known to induce a change in cytokine levels in patients with schizophrenia, which affects regulation of the immune response and inflammatory status. This study investigated the influence of typical and atypical antipsychotics on catalytic antibody activity and the 10 major pro- and anti-inflammatory serum cytokine levels...
April 14, 2023: Biomedicines
https://read.qxmd.com/read/37185730/different-directions-of-effects-of-polyclonal-igg-antibodies-from-patients-with-schizophrenia-and-healthy-individuals-on-cell-death-in-vitro-a-pilot-study
#6
JOURNAL ARTICLE
Elena V Epimakhova, Liudmila P Smirnova, Daria V Kazantseva, Daria A Kamaeva, Svetlana A Ivanova
Numerous studies indicate the involvemen of oxidative stress in the pathogenesis of schizophrenia. It has been shown that the serum pool of antibodies in patients with schizophrenia contains catalytically active antibodies (abzymes) that have a wide range of activities, including redox properties. In the present work, the effects of IgGs-having oxidoreductase activities-isolated from the serum of patients with schizophrenia and healthy individuals were studied in vitro. The IgGs were purified by affinity chromatography followed by an SDS-PAGE analysis of homogeneity in a 4-18% gradient gel...
April 6, 2023: Current Issues in Molecular Biology
https://read.qxmd.com/read/37049736/eae-of-mice-enzymatic-cross-site-specific-hydrolysis-of-h2b-histone-by-iggs-against-h1-h2a-h2b-h3-and-h4-histones-and-myelin-basic-protein
#7
JOURNAL ARTICLE
Andrey E Urusov, Kseniya S Aulova, Pavel S Dmitrenok, Valentina N Buneva, Georgy A Nevinsky
Histones have vital roles in chromatin functioning and gene transcription. At the same time, they are pernicious in intercellular space because they stimulate systemic inflammatory and toxic responses. Myelin basic protein (MBP) is the major protein of the axon myelin-proteolipid sheath. Antibody-abzymes with various catalytic activities are specific features of some autoimmune diseases. IgGs against five individual histones (H2B, H1, H2A, H3, and H4) and MBP were isolated from the blood of experimental autoimmune encephalomyelitis-prone C57BL/6 mice by affinity chromatography...
March 27, 2023: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/36901861/experimental-autoimmune-encephalomyelitis-of-mice-iggs-from-the-sera-of-mice-hydrolyze-mirnas
#8
JOURNAL ARTICLE
Georgy A Nevinsky, Andrey E Urusov, Kseniya S Aulova, Evgeny A Ermakov
It was shown that the spontaneous development of experimental encephalomyelitis (EAE) in C57BL/6 mice occurs due to changes in the profile of bone marrow stem cells differentiation. This leads to the appearance of lymphocytes producing antibodies-abzymes that hydrolyze DNA, myelin basic protein (MBP), and histones. The activity of abzymes in the hydrolysis of these auto-antigens slowly but constantly increases during the spontaneous development of EAE. Treatment of mice with myelin oligodendrocyte glycoprotein (MOG) leads to a sharp increase in the activity of these abzymes with their maximum at 20 days (acute phase) after immunization...
February 23, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/36770997/autoantibody-abzymes-with-catalase-activity-in-experimental-autoimmune-encephalomyelitis-mice
#9
JOURNAL ARTICLE
Andrey E Urusov, Anna S Tolmacheva, Kseniya S Aulova, Georgy A Nevinsky
The exact mechanisms of the evolution of multiple sclerosis are still unknown. At the same time, the development in C57BL/6 mice of experimental autoimmune encephalomyelitis (EAE, simulating human multiple sclerosis) happens as a result of the violation of bone marrow hematopoietic stem cell differentiation profiles integrated with the production of toxic auto-antibodies splitting the basic myelin protein, myelin oligodendrocyte glycoprotein (MOG), histones, and DNA. It has been shown that IgGs from the plasma of healthy humans and autoimmune patients oxidize many different compounds due to their peroxidase (H2 O2 -dependent) and oxidoreductase (H2 O2 -independent) activities...
January 30, 2023: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/36430159/natural-igg-against-s-protein-and-rbd-of-sars-cov-2-do-not-bind-and-hydrolyze-dna-and-are-not-autoimmune
#10
JOURNAL ARTICLE
Anna M Timofeeva, Sergey E Sedykh, Evgeny A Ermakov, Andrey L Matveev, Eva I Odegova, Tatiana A Sedykh, Dmitry N Shcherbakov, Iuliia A Merkuleva, Ekaterina A Volosnikova, Valentina S Nesmeyanova, Nina V Tikunova, Georgy A Nevinsky
Since the onset of the COVID-19 pandemic, numerous publications have appeared describing autoimmune pathologies developing after a coronavirus infection, with several papers reporting autoantibody production during the acute period of the disease. Several viral diseases are known to trigger autoimmune processes, and the appearance of catalytic antibodies with DNase activity is one of the earliest markers of several autoimmune pathologies. Therefore, we analyzed whether IgG antibodies from blood plasma of SARS-CoV-2 patients after recovery could bind and hydrolyze DNA...
November 8, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/36364362/antibodies-abzymes-with-antioxidant-activities-in-two-th-and-2d2-experimental-autoimmune-encephalomyelitis-mice-during-the-development-of-eae-pathology
#11
JOURNAL ARTICLE
Anna S Tolmacheva, Kseniya S Aulova, Andrey E Urusov, Vasiliy B Doronin, Georgy A Nevinsky
The exact mechanisms of multiple sclerosis development are still unknown. However, the development of EAE (experimental autoimmune encephalomyelitis) in Th and 2D2 mice is associated with the infringement of the differentiation profiles of bone marrow hematopoietic stem cells which are bound with the production of compounds that are harmful for human autoantibodies-abzymes that hydrolyze myelin oligodendrocyte glycoprotein, myelin basic protein, and DNA. It showed that autoimmune patients' antioxidant IgG antibodies oxidise some compounds due to their peroxidase (H2 O2 -dependent) and oxidoreductase (H2 O2 -independent) activities more effectively than those in healthy humans can...
November 3, 2022: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/36292926/micrornas-of-milk-in-cells-plasma-and-lipid-fractions-of-human-milk-and-abzymes-catalyzing-their-hydrolysis
#12
JOURNAL ARTICLE
Ivan Yu Kompaneets, Evgeny A Ermakov, Valentina N Buneva, Georgy A Nevinsky
Human milk provides neonates with various components that ensure newborns' growth, including protection from bacterial and viral infections. In neonates, the biological functions of many breast milk components can be very different compared with their functions in the body fluids of healthy adults. Catalytic antibodies (abzymes) that hydrolyze peptides, proteins, DNAs, RNAs, and oligosaccharides were detected, not only in the blood sera of autoimmune patients, but also in human milk. Non-coding microRNAs (18-25 nucleotides) are intra- and extracellular molecules of different human fluids...
October 11, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/36289924/multiple-sclerosis-enzymatic-cross-site-specific-recognition-and-hydrolysis-of-h3-histone-by-iggs-against-h3-h1-h2a-h2b-h4-histones-myelin-basic-protein-and-dna
#13
JOURNAL ARTICLE
Georgy A Nevinsky, Valentina N Buneva, Pavel S Dmitrenok
Histones have a specific key role in the remodeling of chromatin and gene transcription. In the blood, free histones are damage-connected proteins. Myelin basic protein (MBP) is the major component of the myelin-proteolipid sheath of axons. Antibodies possessing enzymatic activities (abzymes, ABZs) are the specific features of several autoimmune pathologies. IgGs against five histones, MBP, and DNA were obtained from the sera of multiple sclerosis (MS) patients using several affinity chromatographies. The sites of H3 histone splitting by Abs against five individual histones, MBP, and DNA were revealed by MALDI mass spectrometry...
October 21, 2022: Biomedicines
https://read.qxmd.com/read/36143234/immunoglobulins-g-of-patients-with-schizophrenia-protects-from-superoxide-pilot-results
#14
JOURNAL ARTICLE
Irina A Mednova, Liudmila P Smirnova, Alisa R Vasilieva, Daria V Kazantseva, Elena V Epimakhova, Nina M Krotenko, Arkadiy V Semke, Svetlana A Ivanova
This study aimed to evaluate the superoxide dismutase (SOD) activity of IgG in patients with schizophrenia. After signing informed consent, we included 67 patients with schizophrenia (34 people with acute schizophrenia and 33 individuals were on outpatient treatment in therapeutic remission) and 14 healthy volunteers. IgGs from blood serum were isolated by affinity chromatography. SOD activity of antibodies was determined spectrophotometrically. We have shown for the first time that IgGs from patients with schizophrenia have SOD activity and this activity is an intrinsic property of antibodies...
September 1, 2022: Journal of Personalized Medicine
https://read.qxmd.com/read/36012448/experimental-autoimmune-encephalomyelitis-of-mice-enzymatic-cross-site-specific-hydrolysis-of-h4-histone-by-iggs-against-histones-and-myelin-basic-protein
#15
JOURNAL ARTICLE
Andrey E Urusov, Kseniya S Aulova, Pavel S Dmitrenok, Valentina N Buneva, Georgy A Nevinsky
Histones play vital roles in chromatin functioning and gene transcription, but in intercellular space, they are harmful due to stimulating systemic inflammatory and toxic responses. Myelin basic protein (MBP) is the most important protein of the axon myelin-proteolipid sheath. Antibodies-abzymes with different catalytic activities are critical and specific features of some autoimmune diseases. Five IgG preparations against histones (H4, H1, H2A, H2B, and H3) and against MBP corresponding to different spontaneous, MOG (myelin oligodendrocyte glycoprotein of mice), and DNA-histones that accelerated onset, acute, and remission stages of experimental autoimmune encephalomyelitis (EAE; model of human multiple sclerosis) development were obtained from EAE-prone C57BL/6 mice by several affinity chromatographies...
August 16, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/36009424/multiple-sclerosis-enzymatic-cross-site-specific-recognition-and-hydrolysis-of-h2a-histone-by-iggs-against-h2a-h1-h2b-h3-histones-myelin-basic-protein-and-dna
#16
JOURNAL ARTICLE
Georgy A Nevinsky, Valentina N Buneva, Pavel S Dmitrienok
Histones have a paramount role in chromatin remodeling and gene transcription. Free histones are damage-associated molecules in the blood; administration of histones to animals drives systemic inflammatory and toxic effects. Myelin basic protein (MBP) is the most crucial component of the axon myelin-proteolipid sheath. Antibodies-abzymes with different enzymatic activities are very toxic and an essential feature of some autoimmune diseases. Electrophoretically homogeneous IgGs against H1, H2A, H2B, H3, H4, MBP, and DNA were derived from sera of multiple sclerosis (MS) patients by several affinity chromatographies...
August 3, 2022: Biomedicines
https://read.qxmd.com/read/36008000/evolution-of-biomarker-research-in-autoimmunity-conditions-for-health-professionals-and-clinical-practice
#17
JOURNAL ARTICLE
Arthur Silverstein, Anton Dudaev, Maria Studneva, John Aitken, Sofya Blokh, Andrew David Miller, Sofia Tanasova, Noel Rose, John Ryals, Christoph Borchers, Anders Nordstrom, Marina Moiseyakh, Arturo SolĂ­s Herrera, Nikita Skomorohov, Trevor Marshall, Alan Wu, R Holland Cheng, Ksenia Syzko, Philip D Cotter, Marianna Podzyuban, William Thilly, Paul David Smith, Paul Barach, Khaled Bouri, Yehuda Schoenfeld, Eiji Matsuura, Veronika Medvedeva, Ilya Shmulevich, Liang Cheng, Paul Seegers, Yekaterina Khotskaya, Keith Flaherty, Steven Dooley, Eric J Sorenson, Michael Ross, Sergey Suchkov
Medical abzymology has made a great contribution to the development of general autoimmunity theory: it has put the autoantibodies (Ab) as the key brick of the theory to the level of physiological functionality by providing such Ab with the ability to catalyze and mediate direct and independent cytotoxic effect on cellular and molecular targets. Natural catalytic autoantibodies (abzymes) while being a pool of canonical Abs and possessing catalytic activity belong to the new group of physiologically active substances whose features and properties are evolutionary consolidated in one functionally active biomolecule...
2022: Progress in Molecular Biology and Translational Science
https://read.qxmd.com/read/35897678/maximally-expressed-mirnas-of-milk-in-cells-plasma-and-lipid-fraction-of-human-milk-and-antibodies-abzymes-catalyzing-their-hydrolysis
#18
JOURNAL ARTICLE
Ivan Yu Kompaneets, Evgeny A Ermakov, Valentina N Buneva, Georgy A Nevinsky
Human milk provides neonates with various components that ensure newborns' growth, including protection from bacterial and viral infections. In neonates, the biological functions of many breast milk components can be very different compared with their functions in the body fluids of healthy adults. Catalytic antibodies-abzymes hydrolyzing peptides, proteins, DNAs, RNAs, and oligosaccharides were detected not only in the blood sera of autoimmune patients but also in human milk. Non-coding microRNAs (18-25 nucleotides) are intra- and extra-cellular molecules of different human fluids...
July 22, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/35806400/catalytic-antibodies-in-bipolar-disorder-serum-iggs-hydrolyze-myelin-basic-protein
#19
JOURNAL ARTICLE
Daria A Kamaeva, Liudmila P Smirnova, Svetlana N Vasilieva, Daria V Kazantseva, Alisa R Vasilieva, Svetlana A Ivanova
The pathogenesis of bipolar affective disorder is associated with immunological imbalances, a general pro-inflammatory status, neuroinflammation, and impaired white matter integrity. Myelin basic protein (MBP) is one of the major proteins in the myelin sheath of brain oligodendrocytes. For the first time, we have shown that IgGs isolated from sera of bipolar patients can effectively hydrolyze human myelin basic protein (MBP), unlike other test proteins. Several stringent criteria were applied to assign the studied activity to serum IgG...
July 2, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/35409256/essential-protective-role-of-catalytically-active-antibodies-abzymes-with-redox-antioxidant-functions-in-animals-and-humans
#20
REVIEW
Anna S Tolmacheva, Georgy A Nevinsky
During the life of aerobic organisms, the oxygen resulting from numerous reactions is converted into reactive oxygen species (ROS). Many ROS are dangerous due to their high reactivity; they are strong oxidants, and react with various cell components, leading to their damage. To protect against ROS overproduction, enzymatic and non-enzymatic systems are evolved in aerobic cells. Several known non-enzymatic antioxidants have a relatively low specific antioxidant activity. Superoxide dismutases, catalase, glutathione peroxidase, glutathione S-transferase, thioredoxin, and the peroxiredoxin families are the most important enzyme antioxidants...
March 31, 2022: International Journal of Molecular Sciences
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