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https://www.readbyqxmd.com/read/30265424/changes-in-cell-differentiation-and-proliferation-lead-to-production-of-abzymes-in-eae-mice-treated-with-dna-histone-complexes
#1
Kseniya S Aulova, Ludmila B Toporkova, Julia A Lopatnikova, Alina A Alshevskaya, Sergey E Sedykh, Valentina N Buneva, Thomas Budde, Sven G Meuth, Nelly A Popova, Irina A Orlovskaya, Georgy A Nevinsky
Experimental autoimmune encephalomyelitis (EAE)-prone C57BL/6 mice are used as a model of human multiple sclerosis. We immunize mice with myelin oligodendrocyte glycoprotein (MOG), DNA-histone and DNA-methylated bovine serum albumin (met-BSA) complexes to reveal different characteristics of EAE development including bone marrow lymphocyte proliferation and differentiation profiles of hematopoietic stem cells. Immunization of C57BL/6 mice with MOG35-55 results in the acceleration of EAE development. Anti-DNA antibodies are usually directed against DNA-histone complexes resulting from cell apoptosis...
September 28, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/30112774/autoimmunity-and-immune-system-dysregulation-in-schizophrenia-iggs-from-sera-of-patients-hydrolyze-myelin-basic-protein
#2
Daria Parshukova, Liudmila P Smirnova, Evgeny A Ermakov, Nikolay A Bokhan, Arkadiy V Semke, Svetlana A Ivanova, Valentina N Buneva, Georgy A Nevinsky
Several different theories of schizophrenia (SCZ) were discussed; the causes of this disease are not yet clear. Using ELISA, it was shown that titers of autoantibodies against myelin basic protein (MBP) in SCZ patients are ~1.8-fold higher than in healthy individuals but 5.0-fold lower than in patients with multiple sclerosis. Several rigid criteria were checked to show that the MBP-hydrolyzing activity is an intrinsic property of SCZ IgGs. Approximately 82% electrophoretically homogeneous SCZ IgGs purified using several affinity sorbents including Sepharose with immobilized MBP hydrolyze specifically only MBP but not many other tested proteins...
August 15, 2018: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/30055664/the-dna-hydrolyzing-activity-of-igg-antibodies-from-human-placenta
#3
Evgenii A Lekchnov, Pavel S Dmitrenok, Ol'ga D Zakharova, Sergey E Sedykh, Valentina N Buneva, Georgy A Nevinsky
OBJECTIVE: Since during pregnancy and lactation women tend to develop autoimmune processes, characterization of placenta immunoglobulins and their possible catalytic functions is a very important step towards understanding the function of placenta. METHODS: ELISA, affinity chromatography, SDS-PAGE and MALDI mass spectrometry were used. RESULTS: It was shown, that ten placentas contain in average 1.1 ± 0.33 mg IgGs/g of placenta and 0...
August 2018: Placenta
https://www.readbyqxmd.com/read/29738685/blood-derived-rna-and-microrna-hydrolyzing-igg-antibodies-in-schizophrenia-patients
#4
E A Ermakov, S A Ivanova, V N Buneva, G A Nevinsky
Abzymes with various catalytic activities are the earliest statistically significant markers of existing and developing autoimmune diseases (AIDs). Currently, schizophrenia (SCZD) is not considered to be a typical AID. It was demonstrated recently that antibodies from SCZD patients efficiently hydrolyze DNA and myelin basic protein. Here, we showed for the first time that autoantibodies from 35 SCZD patients efficiently hydrolyze RNA (cCMP > poly(C) > poly(A) > yeast RNA) and analyzed site-specific hydrolysis of microRNAs involved in the regulation of several genes in SCZD (miR-137, miR-9-5p, miR-219-2-3p, and miR-219a-5p)...
May 2018: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29457292/antibodies-against-h3-and-h4-histones-from-the-sera-of-hiv-infected-patients-catalyze-site-specific-degradation-of-these-histones
#5
Svetlana V Baranova, Pavel S Dmitrenok, Anastasiya D Zubkova, Nikita V Ivanisenko, Elena S Odintsova, Valentina N Buneva, Georgy A Nevinsky
Histones and their posttranslational modified forms play pivotal roles in chromatin functioning and gene transcription. Also, histones are harmful when they enter the intercellular space; their administration to animals results in systemic inflammatory and toxic responses. Autoantibodies having enzymatic activities (abzymes) are the specific feature of several autoimmune and viral diseases. Electrophoretically homogeneous IgGs containing no canonical proteases were purified from sera of HIV-infected patients by using several affinity chromatographies...
July 2018: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/29341394/hydrolysis-by-catalytic-iggs-of-microrna-specific-for-patients-with-schizophrenia
#6
Evgeny A Ermakov, Svetlana A Ivanova, Valentina N Buneva, Georgy A Nevinsky
Significant importance of autoimmune changes in the pathogenesis of schizophrenia (SCZ) is not established. Here, we present the first evidence that autoantibodies of 100% SCZ patients possess RNase activity: сCMP > poly(C) > poly(A) > yeast RNA. In addition, we have got an unexpected result: there was revealed site-specific hydrolysis of four known SCZ specific microRNAs (miR-137, miR-9-5p, miR-219-2-3p, and miR-219a-5p) playing an important role in the regulation of several genes functioning...
February 2018: IUBMB Life
https://www.readbyqxmd.com/read/28780774/changes-in-haematopoietic-progenitor-colony-differentiation-and-proliferation-and-the-production-of-different-abzymes-in-eae-mice-treated-with-dna
#7
Kseniya S Aulova, Ludmila B Toporkova, Julia A Lopatnikova, Alina A Alshevskaya, Sergei V Sennikov, Valentina N Buneva, Thomas Budde, Sven G Meuth, Nelly A Popova, Irina A Orlovskaya, Georgy A Nevinsky
Immunization of experimental autoimmune encephalomyelitis (EAE)-prone C57BL/6 mice with MOG35-55 (a model used to study aspects of human multiple sclerosis) is known to lead to the production of various abzymes. The production of catalytic IgGs that can efficiently hydrolyse myelin basic protein (MBP), MOG and DNA is associated with changes in the profile of differentiation and level of proliferation of mice bone marrow haematopoietic stem cells (HSCs). As MOG simulates the production of abzymes with high DNase activity, we compared the effects of DNA and MOG immunization on EAE-prone mice...
December 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28426042/antibodies-to-h2a-and-h2b-histones-from-the-sera-of-hiv-infected-patients-catalyze-site-specific-degradation-of-these-histones
#8
Svetlana V Baranova, Pavel S Dmitrienok, Nikita V Ivanisenko, Valentina N Buneva, Georgy A Nevinsky
Histones and their post-translational modifications have key roles in chromatin remodeling and gene transcription. Besides intranuclear functions, histones act as damage-associated molecules when they are released into the extracellular space. Administration of histones to animals leads to systemic inflammatory and toxic responses. Autoantibodies with enzymatic activities (abzymes) are distinctive features of some autoimmune and viral diseases. Electrophoretically homogeneous IgGs containing no canonical enzymes were isolated from the sera of HIV-infected patients by chromatography on several affinity sorbents including anti-histone Sepharose...
June 1, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/27862500/antibodies-to-h1-histone-from-the-sera-of-hiv-infected-patients-recognize-and-catalyze-site-specific-degradation-of-this-histone
#9
Svetlana V Baranova, Pavel S Dmitrienok, Nikita V Ivanisenko, Valentina N Buneva, Georgy A Nevinsky
Histones and their posttranslational modifications have key roles in chromatin remodeling and gene transcription. Besides intranuclear functions, histones act as damage-associated molecules when they are released into the extracellular space. Administration of histones to animals leads to systemic inflammatory and toxic responses. Autoantibodies with enzymatic activities (abzymes) are distinctive feature of some autoimmune and viral diseases. Electrophoretically and immunologically homogeneous IgGs containing no canonical enzymes were isolated from sera of human immunodeficiency virus-infected patients by chromatography on several affinity sorbents...
March 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/27552288/site-directed-chemical-mutations-on-abzymes-large-rate-accelerations-in-the-catalysis-by-exchanging-the-functionalized-small-nonprotein-components
#10
Fumihiro Ishikawa, Masato Shirahashi, Hiroshi Hayakawa, Asako Yamaguchi, Takatsugu Hirokawa, Takeshi Tsumuraya, Ikuo Fujii
Taking advantage of antibody molecules to generate tailor-made binding sites, we propose a new class of protein modifications, termed as "site-directed chemical mutation." In this modification, chemically synthesized catalytic components with a variety of steric and electronic properties can be noncovalently and nongenetically incorporated into specific sites in antibody molecules to induce enzymatic activity. Two catalytic antibodies, 25E2 and 27C1, possess antigen-combining sites which bind catalytic components and act as apoproteins in catalytic reactions...
October 21, 2016: ACS Chemical Biology
https://www.readbyqxmd.com/read/27196086/comparison-of-antibodies-with-amylase-activity-from-cerebrospinal-fluid-and-serum-of-patients-with-multiple-sclerosis
#11
COMPARATIVE STUDY
Vasilii B Doronin, Taisiya A Parkhomenko, Massimiliano Castellazzi, Edward Cesnik, Valentina N Buneva, Enrico Granieri, Georgy A Nevinsky
We have recently shown that IgGs from serum and cerebrospinal fluid (CSF) of MS patients are active in hydrolysis of DNA and myelin basic protein. According to literature data, anti-DNA and anti-MBP abzymes may promote important neuropathologic mechanisms in this chronic inflammatory disorder and in MS pathogenesis development. At the same time, the involvement of antibodies with amylase activity in the pathogenesis of any autoimmune disease has not yet been identified. Electrophoretically and immunologically homogeneous IgGs were obtained by a sequential affinity chromatography of the CSF proteins on protein G-Sepharose and FPLC gel filtration...
2016: PloS One
https://www.readbyqxmd.com/read/26799177/antibodies-from-the-sera-of-hiv-infected-patients-efficiently-hydrolyze-all-human-histones
#12
Svetlana V Baranova, Valentina N Buneva, Georgy A Nevinsky
Histones and their post-translational modifications have key roles in chromatin remodeling and gene transcription. Besides intranuclear functions, histones act as damage-associated molecular pattern molecules when they are released into the extracellular space. Administration of exogenous histones to animals leads to systemic inflammatory and toxic responses through activating Toll-like receptors and inflammasome pathways. Here, using ELISA it was shown that sera of HIV-infected patients and healthy donors contain autoantibodies against histones...
August 2016: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/26655427/anti-dna-antibody-mediated-catalysis-is-isotype-dependent
#13
Yumin Xia, Ertan Eryilmaz, Qiuting Zhang, David Cowburn, Chaim Putterman
Anti-DNA antibodies are the serological hallmark of systemic lupus erythematosus, and participate in the pathogenesis of lupus nephritis by cross-reacting with multiple renal antigens. Previously, using a panel of murine anti-DNA IgGs that share identical variable regions but that differ in the constant regions, we demonstrated that the cross-reaction and renal pathogenicity of anti-DNA antibodies are isotype dependent. In this study, we investigated the catalytic potential of this anti-DNA antibody panel, and determined its isotype dependency...
January 2016: Molecular Immunology
https://www.readbyqxmd.com/read/26493273/changes-in-different-parameters-lymphocyte-proliferation-and-hematopoietic-progenitor-colony-formation-in-eae-mice-treated-with-myelin-oligodendrocyte-glycoprotein
#14
Vasilii B Doronin, Taisiya A Parkhomenko, Alexey Korablev, Ludmila B Toporkova, Julia A Lopatnikova, Alina A Alshevskaja, Sergei V Sennikov, Valentina N Buneva, Thomas Budde, Sven G Meuth, Irina A Orlovskaya, Nelly A Popova, Georgy A Nevinsky
Myelin oligodendrocyte glycoprotein (MOG) is an antigen of the myelin sheath, which may trigger immune cell responses and the production of auto-antibodies in multiple sclerosis (MS). In this study, we used MOG(35-55) -induced experimental autoimmune encephalomyelitis (EAE), a model of human MS, to assess the production of catalytically active immunoglobulin G (IgG) antibodies or abzymes which have been shown to be present in sera of patients with several autoimmune diseases. Here, we show that IgGs from the sera of control C57BL/6 mice are catalytically inactive...
January 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/26382278/dna-hydrolysing-activity-of-igg-antibodies-from-the-sera-of-patients-with-schizophrenia
#15
Evgeny A Ermakov, Ludmila P Smirnova, Taisiya A Parkhomenko, Pavel S Dmitrenok, Nina M Krotenko, Nikolai S Fattakhov, Nikolay A Bokhan, Arkadiy V Semke, Svetlana A Ivanova, Valentina N Buneva, Georgy A Nevinsky
It is believed that damage to the membranes of brain cells of schizophrenia (SCZ) patients induces the formation of autoantigens and autoantibodies. Nevertheless, the importance of immunological changes leading to the loss of tolerance to self-antigens in the genesis of SCZ has not been established. The MALDI mass spectra of the IgG light chains of 20 healthy donors were relatively homogeneous and characterized by one peak with only one maximum. In contrast to the healthy donors, the MALDI mass spectra of IgG light chains corresponding to 20 SCZ patients demonstrated, similarly to 20 autoimmune systemic lupus erythematosus (SLE) patients, two maxima of a comparable intensity...
September 2015: Open Biology
https://www.readbyqxmd.com/read/25946706/igg-abzymes-with-peroxidase-and-oxidoreductase-activities-from-the-sera-of-healthy-humans
#16
Anna S Tolmacheva, Elena A Blinova, Evgeny A Ermakov, Valentina N Buneva, Nataliya L Vasilenko, Georgy A Nevinsky
We present the evidence showing that small fractions of electrophoretically homogeneous immunoglobulin G (IgGs) from the sera of healthy humans and their Fab and F(ab)2 fragments oxidize 3,3'-diaminobenzidine through a peroxidase activity in the presence of H2 O2 and through an oxidoreductase activity in the absence of H2 O2 . During purification on protein G-Sepharose and gel filtration, the polyclonal IgGs partially lose the Me(2+) ions. After extensive dialysis of purified Abs against agents chelating metal ions, the relative peroxidase activity decreased dependently of IgG analyzed from 100 to ~10-85%, while oxidoreductase activity from 100 to 14-83%...
September 2015: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/25867248/systemic-lupus-erythematosus-molecular-cloning-and-analysis-of-22-individual-recombinant-monoclonal-kappa-light-chains-specifically-hydrolyzing-human-myelin-basic-protein
#17
Anna M Timofeeva, Valentina N Buneva, Georgy A Nevinsky
Antibodies hydrolyzing myelin basic protein (MBP) can play an important role in the pathogenesis of multiple sclerosis (MS) and systemic lupus erythematosus (SLE). An immunoglobulin light chain phagemid library derived from peripheral blood lymphocytes of patients with SLE was used. Small pools of phage particles displaying light chains with different affinities for MBP were isolated by affinity chromatography on MBP-Sepharose, and the fraction eluted with 0.5 M NaCl was used for preparation of individual monoclonal light chains (MLChs, 26-27 kDa)...
October 2015: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/25756533/features-of-hydrolysis-of-specific-and-nonspecific-globular-proteins-and-oligopeptides-by-antibodies-against-viral-integrase-from-blood-of-hiv-infected-patients
#18
E S Odintsova, P S Dmitrenok, S V Baranova, A M Timofeeva, V N Buneva, G A Nevinsky
It was shown previously that, as differentiated from canonical proteases, abzymes against myelin basic protein (MBP) from blood of patients with multiple sclerosis and systemic lupus erythematosus effectively cleaved only MBP, while antibodies (ABs) against integrase (IN) from blood of HIV-infected patients specifically hydrolyzed only IN. In this work, all sites of effective hydrolysis by anti-IN antibodies (IgG and IgM) of 25-mer oligopeptide (OP25) corresponding to MBP were identified using reversed-phase and thin-layer chromatographies and MALDI mass spectrometry...
February 2015: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/25265393/comparison-of-antibodies-hydrolyzing-myelin-basic-protein-from-the-cerebrospinal-fluid-and-serum-of-patients-with-multiple-sclerosis
#19
Visilii B Doronin, Taisiya A Parkhomenko, Massimiliano Castellazzi, Marina Padroni, Michela Pastore, Valentina N Buneva, Enrico Granieri, Georgy A Nevinsky
It was found that antibodies (Abs) against myelin basic protein (MBP) are the major components of the antibody response in multiple sclerosis (MS) patients. We have recently shown that IgGs from sera of MS patients are active in the hydrolysis of MBP. However, in literature there are no available data concerning possible MBP-hydrolyzing Abs in cerebrospinal fluid (CSF) of MS patients. We have shown that the average content of IgGs in their sera is about 195-fold higher than that in their CSF. Here we have compared, for the first time, the average content of lambda- and kappa-IgGs as well as IgGs of four different subclasses (IgG1-IgG4) in CSF and sera of MS patients...
2014: PloS One
https://www.readbyqxmd.com/read/24992710/two-step-chromatography-purification-of-iggs-possessing-sialidase-activity-from-human-blood-serum
#20
Yury Kit, Rostyslav Bilyy, Nataliya Korniy, Andriy Tomin, Valentyna Chop'yak, Yaroslav Tolstyak, Volodymyr Antonyuk, Rostyslav Stoika
Sialation of cell surface is known to be tightly connected with tumorigenicity, invasiveness, metastatic potential and clearance of aged cells, while sialation of immunoglobulin G (IgG) molecules determines their anti-inflammatory properties. Recently, we have found for the first time IgG-antibodies possessing sialidase-like activity (sialylic abzyme) in blood serum of multiple myeloma and systemic lupus erythematosis patients. This abzyme was detected in a pool of IgGs purified by a typical procedure including immunoglobulin's precipitation with ammonium sulfate and following chromatography on protein G-Sepharose column...
March 2015: Biomedical Chromatography: BMC
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