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https://www.readbyqxmd.com/read/28209721/the-cumulative-burden-of-double-stranded-dna-virus-detection-after-allogeneic-hct-is-associated-with-increased-mortality
#1
Joshua A Hill, Bryan T Mayer, Hu Xie, Wendy M Leisenring, Meei-Li Huang, Terry Stevens-Ayers, Filippo Milano, Colleen Delaney, Mohamed L Sorror, Brenda M Sandmaier, Garrett Nichols, Danielle M Zerr, Keith R Jerome, Joshua T Schiffer, Michael Boeckh
Strategies to prevent active infection with certain double-stranded DNA (dsDNA) viruses after allogeneic hematopoietic cell transplantation (HCT) are limited by incomplete understanding of their epidemiology and clinical impact. We retrospectively tested weekly plasma samples from allogeneic HCT recipients at our center from 2007-2014. We used quantitative PCR to test for cytomegalovirus (CMV), BK polyomavirus (BKV), human herpesvirus 6B (HHV-6B), HHV-6A, adenovirus (AdV), and Epstein-Barr virus (EBV) between days 0-100 post-HCT...
February 16, 2017: Blood
https://www.readbyqxmd.com/read/28207975/stabilization-of-renal-function-after-the-first-year-of-follow-up-in-kidney-transplant-recipients-treated-for-significant-bk-polyomavirus-infection-or-bk-polyomavirus-associated-nephropathy
#2
Marie-Christine Simard-Meilleur, Paule Bodson-Clermont, Gilles St-Louis, Michel R Pâquet, Catherine Girardin, Marie-Chantal Fortin, Héloïse Cardinal, Marie-Josée Hébert, Reneé Lévesque, Edith Renoult
BACKGROUND: BK polyomavirus virus (BKPyV) screening and immunosuppression reduction effectively prevent graft loss due to BKPyV-associated nephropathy (BKPVAN) during the first year after transplantation. The aim of our study was to evaluate the impact of this infection during longer follow-up periods. METHODS: We reviewed the outcome of our screening and immunosuppression reduction protocol in 305 patients who received a kidney transplant between March 2008 and January 2013...
February 16, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/28177804/entry-infection-replication-and-egress-of-human-polyomaviruses-an-update
#3
Soumen Bhattacharjee, Sutanuka Chattaraj
Polyomaviruses (PyVs), belonging to the family Polyomaviridae, are a group of small, nonenveloped, double-stranded, circular DNA viruses widely distributed in the vertebrates. PyVs cause no apparent disease in adult laboratory mice but cause a wide variety of tumors when artificially inoculated into neonates or semipermissive animals. A few human PyVs, such as BK, JC, and Merkel cell PyVs, have been unequivocally linked to pathogenesis under conditions of immunosuppression. Infection is thought to occur early in life and persists for the lifespan of the host...
October 29, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28160087/nationwide-survey-of-bk-polyomavirus-associated-hemorrhagic-cystitis-in-adult-allogeneic-stem-cell-transplantation-among-haematologists-and-urologists
#4
Laila Schneidewind, Thomas Neumann, Jennifer Kranz, Florian Knoll, Alexandre Egon Pelzer, Christian Schmidt, William Krüger
There are no epidemiological data on BK virus associated hemorrhagic cystitis (BKHC) in adult allogeneic stem cell transplantation in Germany available and associations with clinical conditions like GvHD are controversially discussed. Therefore, we conducted a nationwide survey among haematologists and urologists about this disease. We developed two questionnaires, one for haematologists (26 items) and one for urologists (20 items) concerning BKHC in adult allogeneic stem cell transplantation with epidemiological data and clinical implications...
February 3, 2017: Annals of Hematology
https://www.readbyqxmd.com/read/28151406/a-simple-and-reliable-strategy-for-bk-virus-subtyping-and-subgrouping
#5
Virginie Morel, Elodie Martin, Catherine François, François Helle, Justine Faucher, Thomas Mourez, Gabriel Choukroun, Gilles Duverlie, Sandrine Castelain, Etienne Brochot
BK virus-associated diseases in transplant recipients is an emerging issue. However, identification of the various BK virus subtypes/subgroups is a long and delicate process on the basis of the currently available data. We therefore wanted to define a simple and effective one-step strategy to characterize all BK virus strains from the VP1 gene sequence. Based on the analysis of 199 available complete DNA VP1 sequences, phylogenetic trees, alignments and isolated polymorphisms were used to define an effective strategy to distinguish the twelve different BK virus subtypes/subgroups...
February 1, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28150409/bizarre-cell-dysplasia-of-the-cervix
#6
Ondrej Ondič, Radoslav Ferko, Jana Kašpírková, Marián Švajdler, Boris Rýchly, Peter Talarčík, Jiří Bouda, Michal Michal
AIM: The aim of this study was the characterization of a new subtype of high-grade cervical squamous intraepithelial lesion (HSIL) with enlarged cells containing bizarre nuclei: so-called bizarre cell dysplasia (BCD). METHODS: A total of 29 cervical cone biopsy samples of this type of dysplasia were studied. Multi-target polymerase chain reaction and in situ hybridization human papillomavirus (HPV) detection was performed in all cases. BCD was defined as a subtype of HSIL characterized by the presence of large dysplastic cells with abnormal, large pleomorphic nuclei or multinucleation causing nucleomegaly...
February 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28137206/biopsy-proven-bk-virus-associated-nephropathy-clinico-pathologic-correlations
#7
Sarah Jahdali, Noura Al Oudah, Khaled O Alsaad, Hala Kfoury, Salem Qurashi, Abdulla Al Sayyari
OBJECTIVES: Our objective was to study the clinico-pathologic correlations in BK virus nephropathy. MATERIALS AND METHODS: We conducted a retrospective study of all patients with biopsy-proven polyoma (BK) virus infection. We compared their survival and renal outcomes versus BK virus-negative patients with biopsy-proven graft rejection. Histopathologic characterization by a blinded nephropathologist was performed. RESULTS: BK nephropathy was found in 10 patients biopsied for graft dysfunction...
January 30, 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28128912/robotic-assisted-radical-cystoprostatectomy-and-intracorporeal-ileal-conduit-urinary-diversion-for-a-kidney-transplant-recipient
#8
Peter A Caputo, Daniel Ramirez, Matthew Maurice, Ryan Nelson, Onder Kara, Ercan Malkoc, David Goldfarb, Jihad Kaouk
INTRODUCTION AND OBJECTIVES: Robotic assisted radical cystectomy (RARC) is an alternative to open radical cystectomy. As experience is gained with the RARC approach the technique is being applied to more complex surgical cases. We describe here our technique for RARC with intracorporeal ileal conduit urinary diversion for a renal transplant recipient. MATERIALS AND METHODS: The patient is a 60-year old man with high-grade muscle invasive bladder cancer. He has a history of renal failure due to polycystic kidney disease and received a deceased donor renal transplant in 2008...
January 27, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/28128720/surgical-complications-after-pediatric-renal-transplant
#9
Hakan Sözen, Kibriya Fidan, Onur Özen, Oğuz Söylemezoğlu, Aydın Dalgıç
OBJECTIVES: Renal transplant is the most appropriate treatment for both adult and pediatric patients with end-stage renal failure. Here, we analyzed surgical complications after pediatric renal transplant at our center. MATERIALS AND METHODS: We retrospectively analyzed data from patient files and hospital charts of pediatric patients who had renal transplant at our center (Gazi University, Ankara, Turkey). Our immunosuppression protocol, a calcineurin inhibitor-based triple regimen, was applied to all recipients (steroids, mycophenolic acid)...
January 27, 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28113048/large-t-antigen-variants-of-human-polyomaviruses-9-and-12-and-seroreactivity-against-their-n-terminus
#10
Sarah-Verena Korup-Schulz, Claudia Lucke, Rosa Schmuck, Ugo Moens, Bernhard Ehlers
The tumor antigens (TAgs) of mammalian polyomaviruses (PyVs) are key proteins responsible for modulating the host cell cycle and involved in virus replication as well as cell transformation and tumor formation. Here we aimed at identifying mRNA sequences of known and novel TAgs encoded by the recently discovered human polyomaviruses 9 and 12 (HPyV9 and HPyV12) in cell culture. Synthetic viral genomes were transfected into human and animal cell lines. Gene expression occurred in most cell lines, as measured by qPCR of cDNA copies of mRNA encoding major structural protein VP1...
January 22, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28100494/quantification-of-bk-virus-standards-by-quantitative-real-time-pcr-and-droplet-digital-pcr-is-confounded-by-multiple-virus-populations-in-the-who-bkv-international-standard
#11
Allen C Bateman, Alexander L Greninger, Ederlyn E Atienza, Ajit P Limaye, Keith R Jerome, Linda Cook
BACKGROUND: The WHO recently released a BK virus (BKV) international standard. This study evaluated the WHO international standard and commercially available BKV standards by quantitative real-time PCR (qPCR) and droplet digital PCR (ddPCR). METHODS: WHO, Exact Diagnostics, Acrometrix, and Zeptometrix BKV standards were tested by qPCR and ddPCR. Two preparations of NIST BKV clones were also tested. Nucleic acid was extracted with the Roche MP96 and MPLC, followed by quantification in duplicate...
January 18, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28073178/polyclonal-and-monoclonal-antibodies-for-induction-therapy-in-kidney-transplant-recipients
#12
REVIEW
Penny Hill, Nicholas B Cross, A Nicholas R Barnett, Suetonia C Palmer, Angela C Webster
BACKGROUND: Prolonging kidney transplant survival is an important clinical priority. Induction immunosuppression with antibody therapy is recommended at transplantation and non-depleting interleukin-2 receptor monoclonal antibodies (IL2Ra) are considered first line. It is suggested that recipients at high risk of rejection should receive lymphocyte-depleting antibodies but the relative benefits and harms of the available agents are uncertain. OBJECTIVES: We aimed to: evaluate the relative and absolute effects of different antibody preparations (except IL2Ra) when used as induction therapy in kidney transplant recipients; determine how the benefits and adverse events vary for each antibody preparation; determine how the benefits and harms vary for different formulations of antibody preparation; and determine whether the benefits and harms vary in specific subgroups of recipients (e...
January 11, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28053213/calibration-of-bk-virus-nucleic-acid-amplification-testing-to-the-1st-who-international-standard-for-bk-virus
#13
Susanna K Tan, Stephen Milligan, Malaya K Sahoo, Nathaniel Taylor, Benjamin A Pinsky
Significant inter-assay variability in the quantification of BK virus (BKV) DNA precludes establishing broadly applicable thresholds for the management of BKV infection in transplantation. The 1(st) WHO International Standard for BKV (primary standard) was introduced in 2016 as a common calibrator to improve the harmonization of BKV nucleic acid amplification testing (NAAT) and allow comparison of biological measurements worldwide. Here we discuss the evaluation of the Altona RealStar® BKV assay (Altona) and calibration of results to the International Unit (IU) using the Exact Diagnostics® BKV Verification Panel, a secondary standard traceable to the primary standard...
January 4, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28039910/bk-polyomavirus-genomic-integration-and-large-t-antigen-expression-evolving-paradigms-in-human-oncogenesis
#14
Daniel J Kenan, Piotr A Mieczkowski, Eva Latulippe, Isabelle Côté, Harsharan K Singh, Volker Nickeleit
Human polyomaviruses are ubiquitous, with primary infections typically occurring during childhood and subsequent latency that may last a lifetime. Polyomavirus-mediated disease has been described in immunocompromised patients; its relationship to oncogenesis is poorly understood. Here we present deep sequencing data from a high grade BK virus-associated tumor expressing large T antigen. The carcinoma arose in a kidney allograft six years after transplantation. We identified a novel genotype-1a BK polyomavirus (termed "Chapel Hill BK polyomavirus-2″) that was integrated into the BRE gene in chromosome 2 of tumor cells...
December 31, 2016: American Journal of Transplantation
https://www.readbyqxmd.com/read/28007703/immunohistochemistry-is-rarely-justified-for-the-diagnosis-of-viral-infections
#15
Isaac H Solomon, Jason L Hornick, Alvaro C Laga
OBJECTIVES: To determine the utility of immunohistochemistry (IHC) for the diagnosis of viral infections in surgical pathology specimens lacking characteristic viral cytopathic effects. METHODS: Five years of cases at an academic medical center were reviewed for the use of IHC to detect cytomegalovirus (CMV), herpes simplex virus 1 and 2 (HSV-1 and HSV-2), varicella zoster virus (VZV), adenovirus, or polyomavirus (ie, BK or JC). RESULTS: In total, 1,636 viral IHC stains were ordered on 1,099 specimens from 957 cases...
December 21, 2016: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27938318/incisional-infections-after-renal-transplant-outcome-data-from-238-consecutive-recipients
#16
Demetrios Moris, Spyridon Davakis, Kiriaki Kakavia, John Bokos, Spyridon Vernadakis, Konstantinos Diles, Georgios Sotirchos, Alexandros Barlas, Mina Psychogiou, Georgios Zavos
OBJECTIVES: Surgical incision infections, along with urinary tract infections, are among the most common infective complications after kidney transplant. The aim of this retrospective study is to evaluate the incidence and predisposing factors of surgical incision infection development in renal transplant recipients. MATERIALS AND METHODS: Between 1 January 2012 and 31 December 2015, there were 238 consecutive kidney transplant procedures performed in our unit. Of these, 146 patients received deceased donor kidney allografts and 92 had transplants from living related donors...
December 12, 2016: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/27932159/fulminant-clostridium-perfringens-sepsis-in-kidney-transplant-a-case-report
#17
P Mihaylov, J A Powelson, W C Goggins
BACKGROUND: Infections, particularly urinary tract infections, and cardiovascular accidents are the main causes of morbidity and mortality during the 1st year after kidney transplantation (KT). Bacteria and viruses, such as Escherichia coli, Enteroccoci, and Polyoma BK virus are common in the 1st 6 months, so they are controlled routinely. On the other hand, Clostridium perfringens infection is a rare life-threatening condition, associated with a high mortality rate especially in the transplant population, that is not controlled routinely...
November 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27919133/-significance-of-urological-surgical-treatment-for-viral-hemorrhagic-cystitis-after-allogeneic-hematopoietic-stem-cell-transplantation
#18
Kazuhiro Kurosawa, Shinji Urakami, Kazuya Ishiwata, Jinpei Miyagawa, Kazushige Sakaguchi, Masashi Fujioka, Hirokatsu Murata, Naoko Inoshita, Shuichi Taniguchi, Toshikazu Okaneya
This study investigated the significance of urological surgical intervention for viral hemorrhagic cystitis (HC) after allogeneic hematopoietic stem cell transplantation (allo-HSCT). A total of 1, 024 patients underwent allo-HSCT at our medical center between January 2006 and July 2014. In the 6 patients (0.58%) who required urological surgical treatment for viral HC, we retrospectively analyzed patient characteristics and outcomes. Two patients underwent nephrostomy for bilateral hydronephrosis due to bladder tamponade...
November 2016: Hinyokika Kiyo. Acta Urologica Japonica
https://www.readbyqxmd.com/read/27872831/outcomes-of-renal-transplant-recipients-with-bk-virus-infection-and-bk-virus-surveillance-in-the-auckland-region-from-2006-to-2012
#19
Chun-Yuan Hsiao, Helen L Pilmore, Lifeng Zhou, Janak R de Zoysa
AIM: To evaluate incidence, risk factors and treatment outcome of BK polyomavirus nephropathy (BKVN) in a cohort of renal transplant recipients in the Auckland region without a formal BK polyomavirus (BKV) surveillance programme. METHODS: A cohort of 226 patients who received their renal transplants from 2006 to 2012 was retrospectively reviewed. RESULTS: Seventy-six recipients (33.6%) had a BK viral load (BKVL) test and 9 patients (3.9%) developed BKVN...
November 6, 2016: World Journal of Nephrology
https://www.readbyqxmd.com/read/27862417/ureteral-stent-placement-and-immediate-graft-function-are-associated-with-increased-risk-of-bk-viremia-in-the-first-year-after-kidney-transplantation
#20
Joseph G Maliakkal, Daniel C Brennan, Charles Goss, Timothy A Horwedel, Howard Chen, Dennis K Fong, Nikhil Agarwal, Jie Zheng, Kenneth B Schechtman, Vikas R Dharnidharka
Ureteral stent (UrSt) placement has been shown to be a significant independent risk factor for BK viruria, viremia, and BK virus nephropathy. We assessed whether this observation could be validated at our high volume kidney transplant center that has had a strong historical focus on BK virus nephropathy detection. We performed a retrospective case-control study of adults receiving a kidney-only transplant and followed for 1 year between 2004 and 2011 with uniform immunosuppression and use of blood BK virus PCR screening protocol...
November 18, 2016: Transplant International: Official Journal of the European Society for Organ Transplantation
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