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https://www.readbyqxmd.com/read/30316083/trastuzumab-decorated-tpgs-g-chitosan-nanoparticles-for-targeted-breast-cancer-therapy
#1
Abhishesh Kumar Mehata, Shreekant Bharti, Priya Singh, Matte Kasi Viswanadh, Lakshmi Kumari, Poornima Agrawal, Sanjay Singh, Biplob Koch, Madaswamy S Muthu
Breast cancer, up-regulated with human epidermal growth factor receptor type-2 (HER-2) has led to the concept of developing HER-2 targeted anticancer therapeutics. Docetaxel-loaded D-α-tocopherol polyethylene glycol 1000 succinate conjugated chitosan (TPGS-g-chitosan) nanoparticles were prepared with or without Trastuzumab decoration. The particle size and entrapment efficiency of conventional, non-targeted as well as targeted nanoparticles were in the range of 126-186 nm and 74-78% respectively. In-vitro studies on SK-BR-3 cells showed that docetaxel-loaded non-targeted and HER-2 receptor targeted TPGS-g-chitosan nanoparticles have enhanced the cellular uptake and cytotoxicity with a promising bioadhesion property, in comparison to conventional formulation i...
October 4, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30316082/molecular-imprinted-s-nitrosothiols-nanoparticles-for-nitric-oxide-control-release-as-cancer-target-chemotherapy
#2
Tuanwei Liu, Zhenyun Qiao, Jilan Wang, Ping Zhang, Zhide Zhang, Dian-Shun Guo, Xinlin Yang
It is the goal for the development of cancer target chemotherapy with specific recognition, efficient killing the tumor cells and tissues to avoid the intolerable side effects. Molecular imprinted polymer (MIPs) nanoparticles could introduce kinds of specific bio-markers (template molecules) into the nanoparticles with the subsequent removal, leaving special holes in the structure with predictable recognition specificity with cells. Herein, we design and synthesize a kind of sialic acid (SA) over-expressed tumor target hollow double-layer imprinted polymer nanoparticles with S-nitrosothiols for nitric oxide (NO)-releasing as chemotherapy...
October 3, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30316012/neoantigenic-potential-of-complex-chromosomal-rearrangements-in-mesothelioma
#3
Aaron S Mansfield, Tobias Peikert, James B Smadbeck, Julia B M Udell, Enrique Garcia-Rivera, Laura Elsbernd, Courtney L Erskine, Virginia P Van Keulen, Farhad Kosari, Stephen J Murphy, Hongzheng Ren, Vishnu V Serla, Janet L Schaefer Klein, Giannoula Karagouga, Faye R Harris, Carlos Sosa, Sarah H Johnson, Wendy Nevala, Svetomir N Markovic, Aaron O Bungum, Eric S Edell, Haidong Dong, John C Cheville, Marie Christine Aubry, Jin Jen, George Vasmatzis
INTRODUCTION: Malignant pleural mesothelioma is a disease primarily associated with exposure to the carcinogen asbestos. Whereas other carcinogen-related tumors are associated with a high tumor mutation burden, mesothelioma is not. We sought to resolve this discrepancy. MATERIAL AND METHODS: We used mate-pair (n=22), RNA (n=28) and T cell receptor sequencing along with in silico predictions and immunologic assays to understand how structural variants of chromosomes affect the transcriptome...
October 10, 2018: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/30315949/a-new-sensitive-and-versatile-assay-for-quantitative-determination-of-%C3%AE-fluoro-%C3%AE-alanine-afba-in-human-urine-by-using-the-reversed-phase-ultrahigh-performance-tandem-mass-spectrometry-rp-uhplc-ms-ms-system
#4
Cristina Sottani, Elena Grignani, Laura Zaratin, Donatella Santorelli, Emanuele Studioso, Davide Lonati, Carlo A Locatelli, Ornella Pastoris, Sara Negri, Danilo Cottica
A method for the quantitation of α-fluoro-β-alanine (AFBA), the main metabolite of capecitabine (Cape) and 5-fluoruracil (5-FU), is described. Among antineoplastic drugs (ADs), 5-FU and Cape (the new oral prodrug) are the most commonly applied drugs in cancer therapy. The main objective of this study was to develop a reliable method that would be easy to run on a reversed-phase UHPLC system coupled to tandem mass spectrometry. AFBA was derivatized with Sanger's reagent to ensure complete yield of a stable 2,4 dinitrophenil-α-fluoro-β-alanine derivative...
October 10, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/30315937/peroxiredoxin-interaction-with-the-cytoskeletal-regulatory-protein-crmp2-investigation-of-a-putative-redox-relay
#5
Paul E Pace, Alexander V Peskin, Andreas Konigstorfer, Christine J Jasoni, Christine C Winterbourn, Mark B Hampton
Hydrogen peroxide (H2 O2 ) acts as a signaling molecule in cells by oxidising cysteine residues in regulatory proteins such as phosphatases, kinases and transcription factors. It is unclear exactly how many of these proteins are specifically targeted by H2 O2 because they appear too unreactive to be directly oxidised. One proposal is that peroxiredoxins (Prxs) initially react with H2 O2 and then oxidise adjacent proteins via a thiol relay mechanism. The aim of this study was to identify constitutive interaction partners of Prx2 in Jurkat T-lymphoma cells, in which thiol protein oxidation occurs at low micromolar concentrations of H2 O2 ...
October 10, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/30315924/hydroxymethylation-of-protein-encoding-genes-in-the-testes-involved-in-precocious-puberty-of-eriocheir-sinensis
#6
Genliang Li, Hui Qian
To investigate the possible effects of epigenetic modification of testis protein-encoding genes on precocious puberty of Eriocheir sinensis, we used MeDIP-seq and hMeDIP-seq techniques to compare the methylation and hydroxymethylation of 263 E. sinensis protein-encoding genes known in the NCBI database in precocious testes with those in normally developing testes. The results showed that total methylation level of those genes was lower than their total hydroxymethylation level. Moreover, their total hydroxymethylation level in precocious testes was significantly lower than that in normal testes...
October 10, 2018: Gene
https://www.readbyqxmd.com/read/30315892/recent-advances-in-molecular-diagnostics-and-understanding-mechanisms-of-drug-resistance-in-nontuberculous-mycobacterial-diseases
#7
Hee Jae Huh, Su-Young Kim, Byung Woo Jhun, Sung Jae Shin, Won-Jung Koh
Accumulating evidence suggests that human infections caused by nontuberculous mycobacteria (NTM) are increasing worldwide, indicating that NTM disease is no longer uncommon in many countries. As a result of an increasing emphasis on the importance of differential identification of NTM species, several molecular tools have recently been introduced in clinical and experimental settings. These advances have led to a much better understanding of the diversity of NTM species with regard to clinical aspects and the potential factors responsible for drug resistance that influence the different outcomes of NTM disease...
October 10, 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/30315850/mir-150-promotes-angiogensis-and-proliferation-of-endothelial-progenitor-cells-in-deep-venous-thrombosis-by-targeting-srcin1
#8
Wenbin Wang, Xingyang Zhu, Xiaolong Du, Aman Xu, Xiao Yuan, Yanqing Zhan, Mulin Liu, Shuanhu Wang
Venous thromboembolism (VTE), encompassing deep venous thrombosis (DVT) and pulmonary embolism (PE), is the third most common cardiovascular disease. miR-150 is one of important microRNAs which play critical role in various cellular function such as endothelial progenitor cells (EPCs). In this study, we investigate the effect of miR-150 on EPCs function ex vivo and thrombus resolution in vivo. We determined miR-150 expression in EPCs isolated from DVT patients and control subjects by RT-PCR. Potential target of miR-150 was confirmed by bioinformatics analysis and luciferase reporter respectively...
October 10, 2018: Microvascular Research
https://www.readbyqxmd.com/read/30315770/alternative-splice-forms-of-cyld-mediate-ubiquitination-of-smad7-to-prevent-tgfb-signaling-and-promote-colitis
#9
Yilang Tang, Sonja Reissig, Elke Glasmacher, Tommy Regen, Florian Wanke, Alexei Nikolaev, Katharina Gerlach, Vanessa Popp, Khalad Karram, Massimo C Fantini, Jörn M Schattenberg, Peter R Galle, Markus F Neurath, Benno Weigmann, Florian C Kurschus, Nadine Hövelmeyer, Ari Waisman
BACKGROUND & AIMS: The CYLD lysine 63 deubiquitinase gene (CYLD) encodes tumor suppressor protein that is mutated in familial cylindromatosis, and variants have been associated with Crohn's disease (CD). Splice forms of CYLD that lack exons 7 and 8 regulate transcription factors and functions of immune cells. We examined expression of splice forms of CYLD in colon tissues from patients with CD and their effects in mice. METHODS: We performed immunohistochemical analyses of colon tissues from untreated patients with CD and patients without inflammatory bowel diseases (controls)...
October 10, 2018: Gastroenterology
https://www.readbyqxmd.com/read/30315761/preparation-of-stable-tau-oligomers-for-cellular-and-biochemical-studies
#10
Thomas K Karikari, David A Nagel, Alastair Grainger, Charlotte Clarke-Bland, Eric J Hill, Kevin G Moffat
Increasing evidence suggests that small oligomers are the principal neurotoxic species of tau in Alzheimer's disease and other tauopathies. However, mechanisms of tau oligomer-mediated neurodegeneration are poorly understood. The transience of oligomers due to aggregation can compromise the stability of oligomers prepared in vitro. Consequently, we sought to develop an efficient method which maintains the stability and globular conformation of preformed oligomers. This study demonstrates that labeling a single-cysteine form of the pro-aggregant tau four-repeat region (K18) with either Alexa Fluor 488-C5-maleimide or N-ethylmaleimide in reducing conditions stabilizes oligomers by impeding their further aggregation...
October 10, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/30315760/rapid-detection-of-escherichia-coli-o157-h7-by-a-fluorescent-microsphere-based-immunochromatographic-assay-and-immunomagnetic-separation
#11
Qianru Li, Yuexi Yang, Fei Hu, Yanxue Cai, Xiaoyun Liu, Xiaowei He
To ensure food safety and avoid infections by Escherichia coli O157:H7 (E. coli O157:H7), we developed a novel fluorescent microsphere (FM)-based immunochromatography assay (FM-ICA). FMs were conjugated to anti-E. coli O157:H7 monoclonal antibody (MAb) as an ICA probe, Immunomagnetic beads (IM-beads) were prepared by conjugating functionalized magnetic microspheres with the antibody for enrichment and separation of pathogenic bacteria from complex food matrices. Under selected conditions, a standard curve of FM-ICA measurement of E...
October 10, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/30315526/catalpol-inhibits-homocysteine-induced-oxidation-and-inflammation-via-inhibiting-nox4-nf-%C3%AE%C2%BAb-and-grp78-perk-pathways-in-human-aorta-endothelial-cells
#12
Huimin Hu, Changyuan Wang, Yue Jin, Qiang Meng, Qi Liu, Zhihao Liu, Kexin Liu, Xiaoyu Liu, Huijun Sun
Hyperhomocysteinemia (HHCY) has been recognized as an independent risk factor for atherosclerosis and plays a vital role in the development of atherosclerosis. Catalpol, an iridoid glucoside extracted from the root of Rehmannia glutinosa, can produce anti-inflammatory, anti-oxidant, anti-tumor, and dopaminergic neurons protecting effects. This study aimed to determine the protecting effects of catalpol against homocysteine (HCY)-induced injuries in human aortic endothelial cells (HAECs) and uncover the underlying mechanisms: 1...
October 12, 2018: Inflammation
https://www.readbyqxmd.com/read/30315369/paper-based-graphene-oxide-biosensor-coupled-with-smartphone-for-the-quantification-of-glucose-in-oral-fluid
#13
Yuan Jia, Hao Sun, Xiao Li, Dongke Sun, Tao Hu, Nan Xiang, Zhonghua Ni
Rapid, disposable, point-of-care (POC) oral fluid testing has gained considerable attention in recent years as saliva contains biomarker and components of the serum proteome that offer important information on both oral and systemic disease. Microfluidic paper-based analytical devices (μPADs) coupled with smartphone reflectance sensing systems have long been considered to be an effective POC tool for the diagnostics of biomarkers in oral fluid. However, the existing portable systems are limited by the poor color distribution in the detection area as well as not being universally applicable...
October 12, 2018: Biomedical Microdevices
https://www.readbyqxmd.com/read/30315228/reduced-graphene-oxide-triggered-epithelial-mesenchymal-transition-in-a549-cells
#14
Yanyan Liao, Weiyi Wang, Xiaomei Huang, Yongyan Sun, Shen Tian, Peng Cai
Graphene and its derivatives have exhibited wide potential applications in electronics, structural engineering and medicine. However, over utilization and untreated discharge may cause its distribution into environmental as well as biological chain, which raised the concerns of potential health risk as a potential hazard. Accumulating evidence has demonstrated that graphene derivatives induce lung fibrosis in vivo, so overall goal of this study was to explore the molecular mechanisms underlying the pulmonary fibrotic responses of reduced graphene oxide (rGO), using in vitro assays...
October 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30315225/mir-126-negatively-regulates-plk-4-to-impact-the-development-of-hepatocellular-carcinoma-via-atr-chek1-pathway
#15
Jie Bao, Yan Yu, Jianan Chen, Yuting He, Xiaolong Chen, Zhigang Ren, Chen Xue, Liwen Liu, Qiuyue Hu, Juan Li, Guangying Cui, Ranran Sun
Emerging evidence has shown that microRNA-126 (miR-126) is aberrantly downregulated and plays a vital role in carcinogenesis in various cancers, including HCC. However, the underlying biological mechanisms of miR-126 in HCC are still largely unknown. In present study, we found that miR-126 was downregulated both in HCC tissues and cell lines. Low expression level of miR-126 was associated with poor overall survival (OS), late TNM stage and the presence of recurrence. Overexpression of miR-126 significantly decreased cell proliferation, metastasis and promoted apoptosis in vitro...
October 12, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/30315113/-de-novo-nad-synthesis-is-required-for-intracellular-replication-of-coxiella-burnetii-the-causative-agent-of-the-neglected-zoonotic-disease-q-fever
#16
Mebratu A Bitew, Chen Ai Khoo, Nitika Neha, David P De Souza, Dedreia Tull, Nadeeka K Wawegama, Hayley J Newton, Fiona M Sansom
Coxiella burnetii is an intracellular Gram-negative bacterium responsible for the important zoonotic disease Q fever. Improved genetic tools and the ability to grow this bacterium in host cell-free media has advanced the study of C. burnetii pathogenesis, but the mechanisms that allow it to survive inside the hostile phagolysosome remain incompletely understood. Previous screening of a transposon mutant library for replication within HeLa cells has suggested that nadB , encoding a putative L-aspartate oxidase required for de novo NAD synthesis, is needed for intracellular replication...
October 12, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/30314898/long-noncoding-rna-pcat6-functions-as-an-oncogene-by-binding-to-ezh2-and-suppressing-lats2-in-non-small-cell-lung-cancer
#17
Xuefei Shi, Zhili Liu, Zhicong Liu, Xueren Feng, Feng Hua, Xixian Hu, Bin Wang, Kaihua Lu, Fengqi Nie
BACKGROUND: NSCLC (non-small-cell lung cancer) is the leading cause of cancer-related mortality worldwide. Both epigenetic and genetic changes contribute to the initiation, development and metastasis of NSCLC. Recently, accumulating data have begun to support the notion that long noncoding RNAs (lncRNAs) function as new crucial regulators of diverse biological processes, including proliferation, apoptosis and metastasis, and play crucial roles in tumorigenesis. Nevertheless, further study is warranted to comprehensively determine lncRNAs' functions and potential mechanism...
October 9, 2018: EBioMedicine
https://www.readbyqxmd.com/read/30314766/circulating-adipocyte-fatty-acid-binding-protein-levels-predict-the-development-of-subclinical-atherosclerosis-in-type-2-diabetes
#18
Yang Xiao, Xiaoyu Xiao, Aimin Xu, Xiaoyan Chen, Weili Tang, Zhiguang Zhou
OBJECTIVE: The aim of this study was to investigate the prospective association of circulating adipocyte fatty acid-binding protein (A-FABP) levels with the development of subclinical atherosclerosis in patients with type 2 diabetes in an 8-year prospective study. METHODS: A total of 170 patients with newly diagnosed type 2 diabetes were recruited in the study and 133 patients completed the follow-up of 8 years. Baseline plasma A-FABP levels were measured with enzyme-linked immunosorbent assays...
September 7, 2018: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/30314699/lncrna-hotair-upregulates-cox-2-expression-to-promote-invasion-and-migration-of-nasopharyngeal-carcinoma-by-interacting-with-mir-101
#19
Weiqi Hu, Weimin Xu, Yi Shi, Weijun Dai
BACKGROUND: Nasopharyngeal carcinoma (NPC) is the most common type of head and neck cancers which is notable for its distinctive pattern of geographical distribution. HOTAIR has been reported to regulate nasopharyngeal carcinoma tumorigenesis and progression. However, the detailed mechanism underlying HOTAIR-promoted nasopharyngeal carcinoma remains not fully understood. METHODS: We used RT-qPCR approach to examine genes expression and mRNA level. MTT assay and soft agar assay were used to detect cell growth rate in culture and under suspended condition, respectively...
October 10, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/30314650/synergistic-interaction-of-rutin-and-silibinin-on-human-colon-cancer-cell-line
#20
Sana Nafees, Syed Hassan Mehdi, Mohammad Zafaryab, Bushra Zeya, Tarique Sarwar, Moshahid Alam Rizvi
AIM OF THE STUDY: Rutin and Silibinin are active flavonoid compounds, well-known for possessing multiple biological activities. We have studied how Rutin and Silibinin in combination modulate wide range intracellular signaling cascades as evidenced by in-vitro research. Data obtained from preclinical studies provide evidence to be supportive to bridge basic and translational studies. METHODS: In this study, cytotoxic effect of Rutin and Silibinin individually and in combination on the viability of colon cancer cell line (HT-29) was revealed using the MTT assay...
October 9, 2018: Archives of Medical Research
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