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Gold antibody

Huidan Sun, Shaoyan Wu, Xiaoyan Zhou, Min Zhao, Haiping Wu, Rong Luo, Shijia Ding
A sandwich-type of electrochemical immunoassay is described for the determination of insulin. It is based on the use of a glassy carbon electrode that was modified with MoS2 nanosheets decorated with gold nanoparticles (AuNPs) to immobilize a large amount of first antibody (Ab1 ). Following exposure to insulin, secondary antibody (Ab2 ) that was cross-linked to a DNA initiator strand (T0 ) to form an Ab2 @T0 conjugate was added to undergo a sandwich immunoreaction. Subsequently, the long dsDNA concatemer was formed by a hybridization chain reaction between Ab2 @T0 and auxiliary probes (H1 , H2 )...
December 7, 2018: Mikrochimica Acta
Steffen Großhans, Gang Wang, Jürgen Hubbuch
For the purification of biopharmaceutical proteins, liquid chromatography is still the gold standard. Especially with increasing product titers, drawbacks like slow volumetric throughput and high resin costs lead to an intensifying need for alternative technologies. Selective preparative protein precipitation is one promising alternative technique. Although the capability has been proven, there has been no precipitation process realized for large-scale monoclonal antibody (mAb) production yet. One reason might be that the mechanism behind protein phase behavior is not completely understood and the precipitation process development is still empirical...
December 7, 2018: Bioprocess and Biosystems Engineering
Fubin Pei, Ping Wang, Enhui Ma, Qingshan Yang, Haoxuan Yu, Chunxiao Gao, Yueyun Li, Qing Liu, Yunhui Dong
In this work, a sandwich-type electrochemical immunosensor was fabricated to quantitatively detect hepatitis B surface antigen (HBsAg). The immunosensor was based on Rh core and Pt shell nanodendrites loaded onto amino group functionalized graphene nanosheet (RhPt NDs/NH2 -GS) as label and gold nanoparticles loaded onto polypyrrole nanosheet (Au NPs/PPy NS) as platform. RhPt NDs with abundant catalytic active sites because of the branched core-shell structure, RhPt NDs/NH2 -GS as the label displayed high catalytic activity, amplifying the current signal of the immunosensor...
December 1, 2018: Bioelectrochemistry
Su-Lin Yang, Kun-Hsien Tsai, Hsiang-Fei Chen, Jun-Yu Luo, Pei-Yun Shu
Scrub typhus is caused by the intracellular bacterium Orientia tsutsugamushi . The 56-kDa type-specific antigen (TSA) displays a significant antigenic variation across different O. tsutsugamushi strains. To minimize the influence of the antigenic diversity of TSA on assay sensitivity, we developed a mixed-TSA enzyme-linked immunosorbent assay (mixed-TSA ELISA) using a mixture of recombinant TSAs of prototype (Karp, Gilliam, and Kato) and local (TW-1, TW-10, TW-19, and TW-22) O. tsutsugamushi strains as antigens to detect IgM and IgG antibodies against O...
December 10, 2018: American Journal of Tropical Medicine and Hygiene
Fabiani Palagi Machado, Alessandra Rosa Vicari, Fábio Spuldaro, João Batista Saldanha de Castro Filho, Roberto Ceratti Manfro
OBJECTIVE: To investigate the correlation between total lymphocyte and CD3+ T cell counts in peripheral blood in renal transplant patients treated with anti-thymocyte globulin, and discuss related outcomes. METHODS: A single-center, retrospective study involving 226 patients submitted to kidney transplant between 2008 and 2013, and treated with anti-thymocyte globulin for induction or treatment of cellular rejection. Doses were adjusted according to CD3+ T cell or total lymphocyte counts in peripheral blood...
November 29, 2018: Einstein
Xuewen Lu, Ting Mei, Qi Guo, Wenjing Zhou, Xiaomei Li, Jitao Chen, Xinke Zhou, Ning Sun, Zhiyuan Fang
The sensitivity of lateral flow assays (LFA) was increased 30-fold by making use of spherical core-shell gold-silica nanoparticles (AuNP@SiO2 NPs). They can be prepared by silylation of surfactant stabilized AuNPs. The AuNP@SiO2 NPs are highly stable and can be used to label antibodies at virtually any concentration. The detection limit of an LFA for alpha-fetoprotein (AFP) can be decreased from 10 ng·mL-1 to 300 pg·mL-1 which makes it comparable to an enzyme-linked immunosorbent assay. To demonstrate the applicability to an immunoassay, a sandwich assay was developed for vanillin by covalent modification of the AuNP@SiO2 NPs with antibody...
December 4, 2018: Mikrochimica Acta
Rosette Megnekou, Jean Claude Djontu, Benderli C Nana, Jude D Bigoga, Maurice Fotso, Balotin Fogang, Rose F G Leke
BACKGROUND: Plasmodium falciparum infected erythrocytes sequestering in placental tissue release Plasmodium lactate dehydrogenase (pLDH) and histidine-rich protein-II (HRP-II). These proteins can be detected in peripheral blood using monoclonal antibody-based rapid diagnostic tests (RDTs). Nevertheless, studies to evaluate the reliability of RDTs in detecting placental malaria compared with microscopy of placental tissue impression smear (PTIS) as the gold standard are scarce. METHODS: Between August 2013 and January 2015, Giemsa-stained blood smears for peripheral blood smear (Pbs), placental intervillous space (IVS) blood smear and placental tissue impression smear (PTIS)] were prepared from HIV-negative women during delivery at the Marie Reine Medical Health Centre in Yaoundé, Cameroon...
December 4, 2018: Malaria Journal
Nam Hee Lee, Seung-Hoon Nahm, Insung S Choi
The possibility of exposure to botulinum neurotoxin (BoNT), a powerful and potential bioterrorism agent, is considered to be ever increasing. The current gold-standard assay, live-mouse lethality, exhibits high sensitivity but has limitations including long assay times, whereas other assays evince rapidity but lack factors such as real-time monitoring or portability. In this study, we aimed to devise a novel detection system that could detect BoNT at below-nanomolar concentrations in the form of a stretchable biosensor...
December 3, 2018: Sensors
Kim Appler, Scott Brunt, Jodie A Jarvis, April D Davis
OBJECTIVES: Each year, rabies virus infection results in the death of more than 50 000 persons worldwide. In the United States, the Centers for Disease Control and Prevention (CDC) reported 23 human rabies cases from May 1, 2008, through October 1, 2017. Although rabies testing in the United States is highly reliable, some specimens submitted to rabies laboratories do not have adequate tissues or may be substantially decomposed. In these instances, the specimen may be considered unsatisfactory for testing or produce indeterminate results using the gold standard direct fluorescent antibody test...
December 3, 2018: Public Health Reports
V Qaradaghi, B Dousti, Y Choi, G S Lee, W Hu, S Pourkamali
This work presents fabrication of thermal-piezoresistive nanoelectromechanical (NEM) silicon disk resonators and their characterization as highly sensitive mass sensors. Forest of multiwall carbon nanotubes (MWCNT) has been grown on top surface of the fabricated devices increasing the resonator effective surface area, which in turn increases the adsorption capacity and therefore frequency shift of the sensor in molecular or particulate detection applications. To investigate the effect of the enhanced surface area on frequency shift, devices with and without MWCNTs were exposed to an aqueous solution of manganese sulfate for different deposition times and the resonance frequency shift was recorded accordingly...
November 30, 2018: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Ye Hu, Yajiao Huang, Zhengguo Wang, Yanying Wang, Xiaoxue Ye, WingLeung Wong, Chunya Li, Dong Sun
Tungsten disulfide (WS2 ) nanosheets were obtained by exfoliating WS2 bulk crystals in N-methylpyrrolidone by ultrasonication. Gold nanoparticles (GNPs) were synthesized by in-situ ultrasonication of sodium citrate and HAuCl4 while fabricating the WS2 nanosheets. In this way, the GNPs were self-assembled on WS2 nanosheets to form a GNPs/WS2 nanocomposite through interaction between sulfur and gold atoms. The photoelectrochemical response of WS2 nanosheets is significantly enhanced after integration of the GNPs...
December 1, 2018: Mikrochimica Acta
Maneesha K Suresh, Raja Biswas, Lalitha Biswas
Staphylococcus aureus (S. aureus) readily forms biofilms on prosthetic devices such as the pacemakers, heart valves, orthopaedic implants, and indwelling catheters. Its biofilms are recalcitrant to antibiotic therapy and pose a serious burden in the healthcare setting as they drastically increase the treatment cost and morbidity of the patient. Prevention and treatment of staphylococcal biofilms has therefore been an area of active research for the past two decades. While catheters coated with different antiseptics and antibiotics capable of preventing S...
November 27, 2018: International Journal of Medical Microbiology: IJMM
Mario Merone, Carlo Sansone, Paolo Soda
BACKGROUND AND OBJECTIVE: The indirect immunofluorescence (IIF) on HEp-2 cells is the recommended technique for the detection of antinuclear antibodies. However, it is burdened by some limitations, as it is time consuming and subjective, and it requires trained personnel. In other fields the adoption of deep neural networks has provided an effective high-level abstraction of the raw data, resulting in the ability to automatically generate optimized high-level features. METHODS: To alleviate IIF limitations, this paper presents a computer-aided diagnosis (CAD) system classifying HEp-2 fluorescence intensity: it represents each image using an Invariant Scattering Convolutional Network (Scatnet), which is locally translation invariant and stable to deformations, a characteristic useful in case of HEp-2 samples...
November 28, 2018: Artificial Intelligence in Medicine
Zainia Rehmat, Waleed S Mohammed, Muhammad Bilal Sadiq, Manjunath Somarapalli, Anil Kumar Anal
This study demonstrates the evaluation of ochratoxin A (OTA) in coffee on compact surface plasmon resonance (SPR) biosensors based on crosslinked chitosan and carboxymethyl chitosan nanomatrix substrates. Ochratoxin A is a toxic secondary metabolite widely produced by Aspergillus and Penicillium fungi and requires regular quantification and detection in food samples. The gold coated SPR chips were synthesized with chitosan and carboxymethyl chitosan through spin coating technique. The SPR nanomatrix chips were used for the immobilization of ochratoxin A-bovine serum albumin (OTA-BSA) conjugate to develop a competitive inhibition immunoassay...
November 26, 2018: Colloids and Surfaces. B, Biointerfaces
Diandian Deng, Hong Yang, Chang Liu, Kang Zhao, Jianguo Li, Anping Deng
An immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for ultrasensitive determination of Sudan I in food samples was reported. Gold-silver core-shell bimetallic nanorods (referred to as Au@Ag NRs) were synthesized, characterized and used as the substrate for preparation of the ICA. Polyclonal antibody against Sudan I was immobilized on the surface of the Au@Ag NRs carrying the Raman reporter 5,5'-dithiobis (2-nitrobenzoic acid). The Raman scattering intensity on the test line was used for quantitation of Sudan I...
March 30, 2019: Food Chemistry
Muhammad Omar Shaikh, Pei-Yu Zhu, Cheng-Chien Wang, Yi-Chun Du, Cheng-Hsin Chuang
In this study, we have fabricated a simple disposable electrochemical immunosensor for the point of care testing of microalbuminuria, a well-known clinical biomarker for the onset of chronic kidney disease. The immunosensor is fabricated by screen-printing carbon interdigitated microelectrodes on a flexible plastic substrate and utilizes electrochemical impedance spectroscopy to enable direct and label free immunosensing by analyzing interfacial changes on the electrode surface. To improve conductivity and biocompatibility of the screen-printed electrodes, we have modified it with gold nanoparticles, which are electrodeposited using linear sweep voltammetry...
November 22, 2018: Biosensors & Bioelectronics
Yeong-Lim Kang, Ji-Yun Jeong, Hwi-Yeon Choi, Yanhong Zhang, Yumei Liu, Ho-Jong Lee, Jong-Chul Choi, So-Hyun Lee, Beom-Joo Lee, Sang-Won Lee, Joong-Bok Lee, Ki-Hyun Cho, Seung-Yong Park
BACKGROUND: Foot-and-mouth disease (FMD) can be controlled by either stamping out or vaccination, a choice which depends on both the economic importance of the livestock sector as well as the disease status. In FMD-free countries with vaccination, such as Korea, vaccination programs should guarantee prevention against transmission of FMD. Monitoring of vaccination programs is also essential for ensuring sufficient coverage that will limit the transmission of FMDV. There are several methods to screen FMD virus (FMDV) structural protein (SP) antibodies including SPCE (Solid-phase competitive ELISA), LPBE (Liquid-phase blocking ELISA), and VNT (Virus neutralization test)...
November 28, 2018: BMC Veterinary Research
M Y Li, D Q Zhang, X Lu, W C Chen
Objective: To compare the diagnostic value of the old "ABC" method [serum pepsinogen(PG) combined with Helicobacter pylori (Hp) IgG antibody] and the new "ABC" method [serum pepsinogen plus gastrin-17(G-17)] in screening gastric cancer and its precancerous condition. Methods: Serum PG, G-17 and Hp-IgG were quantified by enzyme-linked immunosorbent assay (ELISA) in 278 subjects. Subjects were grouped according to the criteria of two methods. The gastroscopy and pathological biopsy were gold standard...
December 1, 2018: Zhonghua Nei Ke za Zhi [Chinese Journal of Internal Medicine]
Gaozhe Cai, Siyuan Wang, Lingyan Zheng, Jianhan Lin
Immunomagnetic separation has been widely used for the separation and concentration of foodborne pathogens from complex food samples, however it can only handle a small volume of samples. In this paper, we presented a novel fluidic device for the specific and efficient separation and concentration of salmonella typhimurium using self-assembled magnetic nanoparticle chains. The laminated sawtooth-shaped iron foils were first mounted in the 3D-printed matrix and magnetized by a strong magnet to generate dot-array high gradient magnetic fields in the fluidic channel, which was simulated using COMSOL (5...
November 27, 2018: Micromachines
Riikka Peltomaa, Francisco Amaro-Torres, Sergio Carrasco, Guillermo Orellana, Elena Benito-Peña, María C Moreno-Bondi
Homogeneous immunoassays represent an attractive alternative to traditional heterogeneous assays due to their simplicity, sensitivity, and speed. On the basis of a previously identified epitope-mimicking peptide, or mimotope, we developed a homogeneous fluorescence quenching immunoassay based on gold nanoparticles (AuNPs) and a recombinant epitope-mimicking fusion protein for the detection of mycotoxin fumonisin B1 (FB1 ). The fumonisin mimotope was cloned as a fusion protein with a yellow fluorescent protein that could be used directly as the tracer for FB1 detection without the need of labeling or a secondary antibody...
November 27, 2018: ACS Nano
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