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https://www.readbyqxmd.com/read/30114227/generation-and-characterisation-of-recombinant-fmdv-antibodies-applications-for-advancing-diagnostic-and-laboratory-assays
#1
Gareth Shimmon, Abhay Kotecha, Jingshan Ren, Amin S Asfor, Joseph Newman, Stephen Berryman, Eleanor M Cottam, Sarah Gold, Toby J Tuthill, Donald P King, Emiliana Brocchi, Andrew M Q King, Ray Owens, Elizabeth E Fry, David I Stuart, Alison Burman, Terry Jackson
Foot-and-mouth disease (FMD) affects economically important livestock and is one of the most contagious viral diseases. The most commonly used FMD diagnostic assay is a sandwich ELISA. However, the main disadvantage of this ELISA is that it requires anti-FMD virus (FMDV) serotype-specific antibodies raised in small animals. This problem can be, in part, overcome by using anti-FMDV monoclonal antibodies (MAbs) as detecting reagents. However, the long-term use of MAbs may be problematic and they may need to be replaced...
2018: PloS One
https://www.readbyqxmd.com/read/30111640/rheumatological-assessment-is-important-for-interstitial-lung-disease-diagnosis
#2
Yair Levi, Lilach Israeli-Shani, Michael Kuchuk, Gali Epstein Shochet, Matthew Koslow, David Shitrit
OBJECTIVE: Interstitial lung diseases (ILD) form a diverse group of parenchymal lung disorders. Currently, a multidisciplinary team (MDT) including pulmonologists, radiologists, and pathologists is the gold standard for ILD diagnosis. Recently, additional subtypes of connective tissue disease (CTD)-ILD with autoimmune features were defined, making the rheumatological assessment increasingly important. We aimed to assess the effect of adding a rheumatologist to the MDT for routine rheumatology assessment...
August 15, 2018: Journal of Rheumatology
https://www.readbyqxmd.com/read/30109381/gold-nanoparticles-as-labels-for-immunochemical-analysis-using-laser-ablation-inductively-coupled-plasma-mass-spectrometry
#3
Michaela Tvrdonova, Marcela Vlcnovska, Lucie Pompeiano Vanickova, Viktor Kanicky, Vojtech Adam, Lena Ascher, Norbert Jakubowski, Marketa Vaculovicova, Tomas Vaculovic
In this paper, we describe the labelling of antibodies by gold nanoparticles (AuNPs) with diameters of 10 and 60 nm with detection by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Additionally, the AuNPs labelling strategy is compared with commercially available labelling reagents based on MeCAT (metal coded affinity tagging). Proof of principle experiments based on dot blot experiments were performed. The two labelling methods investigated were compared by sensitivity and limit of detection (LOD)...
August 14, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/30101217/modular-protein-engineering-approach-to-the-functionalization-of-gold-nanoparticles-for-use-in-clinical-diagnostics
#4
Timothy Robson, Deepan S H Shah, Alexandra S Solovyova, Jeremy H Lakey
Functional protein-gold nanoparticle (AuNP) conjugates have a wide variety of applications including biosensing and drug delivery. Correct protein orientation, which is important to maintain functionality on the nanoparticle surface, can be difficult to achieve in practice, and dedicated protein scaffolds have been used on planar gold surfaces to drive the self-assembly of oriented protein arrays. Here we use the transmembrane domain of Escherichia coli outer membrane protein A (OmpATM ) to create protein-AuNP conjugates...
July 27, 2018: ACS applied nano materials
https://www.readbyqxmd.com/read/30100448/rapid-detection-of-%C3%AE-conglutin-with-a-novel-lateral-flow-aptasensor-assisted-by-immunomagnetic-enrichment-and-enzyme-signal-amplification
#5
Zhengzong Wu, Deyun He, Enbo Xu, Aiquan Jiao, Muhammad Farhan Jahangir Chughtai, Zhengyu Jin
A simple, rapid and economic lateral flow immunochromatographic assay (LFICA) was designed for ultrasensitive detection of β-conglutin. Instead of antibodies and gold nanoparticles (AuNPs) used in conventional LFICA, a cognate aptamer duo, binding to β-conglutin and Fe3 O4 @Au core-shell nanoparticles, was applied in this study. An enzyme signal amplification strategy was used to enhance sensitivity. In addition, a new magnetic enrichment strategy was employed to further enhance sensitivity of the assay, slowing down movement of the capture probe (i...
December 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/30099200/size-dependent-neutralizing-activity-of-gold-nanoparticle-based-subunit-vaccine-against-dengue-virus
#6
Quang Huy Quach, Swee Kim Ang, Jang-Hann Justin Chu, James Chen Yong Kah
Dengue results in substantial human morbidity and significant socio-economic impacts, but a specific dengue therapeutic is not available. The currently available dengue vaccine has low efficacy and high rate of adverse effects, necessitating different strategies for the development of a safer and more efficient vaccine against dengue virus. We describe here a hybrid combination of different-sized gold nanoparticles (AuNPs) and domain III of envelope glycoprotein derived from serotype 2 of dengue virus (EDIII) as dengue subunit vaccine...
August 9, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/30096012/anti-cd20-monoclonal-antibodies-reviewing-a-revolution
#7
Jml Casan, J Wong, M J Northcott, S Opat
Since the inception of rituximab in the 1990s, anti-CD20 monoclonal antibodies have revolutionised the treatmant of B cell hematological malignancies and have become a cornerstone of modern gold-standard practice. Additionally, the potent efficacy of these agents in depleting the B cell compartment has been used in the management of a broad array of autoimmune diseases. Multiple iterations of these agents have been investigated and are routinely used in clinical practice. In this review, we will discuss the physiology of CD20 and its attractiveness as a therapeutic target, as well as the pharmacology, pre-clinical and clinical data for the major anti-CD20 monoclonal antibodies: rituximab, obinutuzumab and ofatumumab...
August 10, 2018: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/30087938/quantifying-bound-and-active-antibodies-conjugated-to-gold-nanoparticles-a-comprehensive-and-robust-approach-to-evaluate-immobilization-chemistry
#8
Kiran Tripathi, Jeremy D Driskell
Gold nanoparticles (AuNPs) functionalized with antibodies have the potential to improve biosensing technology because of the unique optical properties of AuNPs and the specificity of antibody-antigen interactions. Critical to the development and optimization of these AuNP-enabled sensing technologies is the immobilization of the antibody onto the AuNP. The development of novel immobilization strategies that optimize antibody loading and orientation in an effort to enhance antibody activity, and therefore assay performance, has been the focus of many recent studies...
July 31, 2018: ACS Omega
https://www.readbyqxmd.com/read/30086926/new-system-for-the-detection-of-legionella-pneumophila-in-water-samples
#9
Noemí Párraga-Niño, Sara Quero, Alba Ventós-Alfonso, Naroa Uria, Oscar Castillo-Fernandez, Josune J Ezenarro, Francesc-Xavier Muñoz, Marian Garcia-Nuñez, Miquel Sabrià
Waterborne pathogens are a global concern for public health worldwide. Despite continuing efforts to maintain water safety, water quality is still affected by deterioration and pollution. Legionella pneumophila colonizes man-made water systems and can infect humans causing Legionnaire's disease (LD), pneumonia. The prevention of LD is a public health issue and requires specific systems to control and detect these microorganisms. Culture plate is the only technique currently approved, but requires more than 10 days to obtain results...
November 1, 2018: Talanta
https://www.readbyqxmd.com/read/30085658/multiplexed-sers-detection-of-soluble-cancer-protein-biomarkers-with-gold-silver-alloy-nanoboxes-and-nanoyeast-single-chain-variable-fragments
#10
Junrong Li, Jing Wang, Yadveer Singh Grewal, Christopher B Howard, Lyndon J Raftery, Stephen M Mahler, Yuling Wang, Matt Trau
Highly sensitive, multiplexed detection of soluble cancer protein biomarkers can facilitate early cancer screening as well as enable real-time monitoring of patients' sensitivity and resistance to therapy. Current technologies for detection of soluble cancer protein biomarkers, however, suffer from limited sensitivity e.g., enzyme-linked immunosorbent assay (ELISA), as well as the requirement of expensive monoclonal antibodies (mAbs), which undergo the quality variability. Herein, we propose a sensitive, cheap and robust surface-enhanced Raman scattering (SERS) technology to detect a panel of soluble cancer protein biomarkers, including soluble programmed death 1 (sPD-1), soluble programmed death-ligand 1 (sPD-L1) and soluble epithermal growth factor receptor (sEGFR), which are related to disease progression and treatment efficacy...
August 7, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30081627/atp-bioluminescence-based-bacteria-detection-using-targeted-photothermal-lysis-by-gold-nanorods
#11
Seong U Kim, Eun-Jung Jo, Yuseon Noh, Hyoyoung Mun, Young-Deok Ahn, Min-Gon Kim
Bacterial infections are common causes of morbidity and mortality worldwide; therefore, environmental contamination by bacterial pathogens represents a global public health concern. Consequently, a selective, rapid, sensitive, and in-field detection platform for detecting significant bacterial contamination is required to ensure hygiene and protect public health. Here, we developed a fast and simple platform for the selective and sensitive detection of bacteria by measuring adenosine triphosphate (ATP) bioluminescence following targeted photothermal lysis mediated by antibody conjugated gold nanorods...
August 7, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30081127/ultrasensitive-immunoassay-of-carcinoma-antigen-125-in-untreated-human-plasma-samples-using-gold-nanoparticles-with-flower-like-morphology-a-new-platform-in-early-stage-diagnosis-of-ovarian-cancer-and-efficient-management
#12
Mohammad Hasanzadeh, Rahimeh Sahmani, Elham Solhi, Ahad Mokhtarzadeh, Nasrin Shadjou, Soltanali Mahboob
Ovarian cancer, as one of the most life-threatening malignancies among women worldwide, is usually diagnosed at the late stage despite up regulation of molecular markers such as carcinoma antigen 125 (CA 125) at the early stages of the malignancy. CA 125 is the only tumor marker recommended for clinical use in the diagnosis and management of ovarian cancer. The potential role of CA-125 for the early detection of ovarian cancer is controversial and has not yet been adopted for widespread screening efforts in asymptomatic women...
August 3, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/30078622/electrochemical-aptasensor-using-optimized-surface-chemistry-for-the-detection-of-mycobacterium-tuberculosis-secreted-protein-mpt64-in-human-serum
#13
Marzhan Sypabekova, Pawan Jolly, Pedro Estrela, Damira Kanayeva
Tuberculosis (TB) remains one of the leading causes of mortality worldwide. There is a great need for the development of diagnostic tests, which are reliable, sensitive, stable, and low cost to enable early diagnosis of TB in communities with scarce resources. This study reports the optimization and evaluation of a synthetic receptor, an aptamer, for the detection of the secreted protein MPT64, which is a highly immunogenic polypeptide of Mycobacterium tuberculosis, a causative agent of TB. The study investigates combinatorial effects of an aptamer linker and a co-adsorbent onto a gold electrode for optimal binding efficiency and reduced non-specific interactions for label-free detection of MPT64 using electrochemical impedance spectroscopy...
July 26, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/30073348/resveratrol-delays-retinal-ganglion-cell-loss-and-attenuates-gliosis-related-inflammation-from-ischemia-reperfusion-injury
#14
Hongdou Luo, Jiejie Zhuang, Piaopiao Hu, Wei Ye, Shanshan Chen, Yulian Pang, Ningfeng Li, Cong Deng, Xu Zhang
Purpose: Resveratrol has been shown to enhance the survival of retinal ganglion cells (RGCs) following ischemia-reperfusion (I/R) injury for glaucoma. However, the precise mechanisms for resveratrol's protective effects are still unclear. The aim of this study is to determine whether resveratrol can inhibit RGC apoptosis, retinal gliosis, and inflammation, all of which are critical events in retinal degeneration following I/R injury. Methods: Right retinal ischemia was induced in adult male Sprague Dawley rats by increasing intraocular pressure to 110 mm Hg for 60 minutes, and the left eyes maintained at normal pressure serve as the control...
August 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/30064753/a-peptide-maltose-binding-protein-fusion-protein-used-to-replace-the-traditional-antigen-for-immunological-detection-of-deoxynivalenol-in-food-and-feed
#15
Yang Xu, Hongwei Yang, Zhibing Huang, Yanping Li, Qinghua He, Zhui Tu, Yanwei Ji, Wenjie Ren
A deoxynivalenol (DON) epitope clone (D8 ) was obtained by phage display technology using anti-DON monoclonal antibodies as a target molecule. Subsequently, a DON antigen mimic (D8 -maltose-binding protein [MBP]) was synthesized by fusing the mimic epitope peptide with MBP. An enzyme-linked immunosorbent assay (ELISA) and urchin-like gold nanoparticle immunochromatographic assay was developed based on D8 -MBP for detection of DON in maize and wheat. The half-maximal inhibitory concentration, lower detection limit, and linear range of the D8 -MBP ELISA were 57...
December 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/30062865/fabrication-of-oligomeric-avidin-scaffolds-for-valency-controlled-surface-display-of-functional-ligands
#16
Hye Ryeon Yoon, Hyeong Joo Choi, Yoon-Aa Choi, Jung A Kim, Juyeon Jung, Ho Min Kim, Yongwon Jung
Multivalent surface display of biomolecules is crucial to study and utilize multivalent biological interactions. However, precise valency control of surface displayed ligands remains extremely difficult. Here, we fabricated a series of new oligomeric avidin proteins that allow facile control of surface multivalency of biotinylated ligands. Naturally dimeric rhizavidin (RA) was engineered to form a mixture of oligomeric avidin assemblies, and discrete RA oligomers, from the dimer to octamer of RA, were homogeneously prepared...
July 30, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/30060712/a-surrogate-assay-for-measuring-coxsackievirus-a6-neutralizing-antibodies
#17
Yao Su, Pan Chen, Fan Gao, Lianlian Bian, Shiyang Sun, Fangyu Dong, Yalin Hu, Qunying Mao, Wei Jiang, Xing Wu, Zhenglun Liang
Coxsackievirus A6 (CV-A6) is one of pathogens causing hand, foot and mouth disease (HFMD) and becomes a new challenge to HFMD control. In this study, we first built a single-round pseudovirus infection system for CV-A6, and then developed a pseudovirus luciferase assay (PVLA) for anti-CV-A6 neutralizing antibody (NtAb) quantification. Since cytopahtic effect (CPE) is considered as the gold standard test for anti-enterovirus NtAb detection, a comparison study has been performed using 318 clinical serum samples, as measured both by PVLA and CPE...
July 30, 2018: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/30060625/studies-towards-hctni-immunodetection-using-electrochemical-approaches-based-on-magnetic-microbeads
#18
Alejandro Hernández-Albors, Gloria Colom, J-Pablo Salvador, M-Pilar Marco
Different electrochemical strategies based on the use of magnetic beads are described in this work for the detection of human cardiac troponin I (hcTnI). hcTnI is also known as the gold standard for acute myocardial infarction (AMI) diagnosis according to the different guidelines from the European Society of Cardiology (ESC) and the American College of Cardiology (ACC). Amperometric and voltamperometric sandwich magnetoimmunoassays were developed by biofunctionalization of paramagnetic beads with specific antibodies...
July 29, 2018: Sensors
https://www.readbyqxmd.com/read/30057889/evaluation-of-two-rapid-immunochromatographic-tests-for-diagnosis-of-brucellosis-infection-in-cattle
#19
Anitza Fragas Quintero, Dervel Felipe Díaz Herrera, Dayamí Martín Alfonso, Yanelis Cruz Santana, Raisa Betancourt Torres, Lucy Montano Tamayo
Brucellosis is a serious infectious disease that causes significant economic losses in the livestock industry. Its early diagnosis allows an adequate disease control in cattle. DAVIH Laboratories designed a lateral flow immunochromatographic assay using protein A-colloidal gold as a detector reagent (LFIA-PA). The objective of this work was to compare the performance of this assay using protein G-colloidal gold (LFIA-PG) with its performance using protein A-colloidal gold as the detector reagent. The assays were carried out with 20 μL of serum and 130 μL of running buffer...
2018: Open Veterinary Journal
https://www.readbyqxmd.com/read/30056940/immunostick-elisa-for-rapid-and-easy-diagnosis-of-myasthenia-gravis
#20
Nikolaos Trakas, Socrates J Tzartos
A rapid and simple immunostick immunosorbent ELISA has been developed for the diagnosis of myasthenia gravis. It is based on coating immunosticks with the major myasthenia gravis autoantigens acetylcholine receptor (AChR) or MuSK and it can be performed in <1 h, in a non-specialized examination place by non-skilled personnel. The resulting chromogen precipitate on the sticks, evaluated by eye, was proved a reliable indication of the presence of autoantigen-specific autoantibodies. Although this assay is mostly qualitative, compared to the results obtained from the gold-standard radioimmunoprecipitation assays, its specificity for anti-AChR antibodies was 99...
September 2018: Journal of Immunological Methods
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