Read by QxMD icon Read

Gold antibody

Gerhild Wildner
In contrast to rats, mouse models are nowadays generally used for the investigation of immune responses and immune-mediated diseases, there are many different strains and mouse-specific tools available, and it is easy to generate transgenic and constitutive or inducible knockout mice for any gene. Many immune markers and mechanisms have been detected in mice and have been introduced as gold standard in immunology, however, some turned out to be not unconditionally transferable to the human immune system. Rats have been used more frequently in former days but are mostly outstripped by mice due to the fact that fewer strains are available, they need more space than mice, are more expensive to maintain and breed, and it is extremely difficult to generate transgenic or ko-rats...
October 12, 2018: Immunobiology
Bing Sun, Jing Dong, Lin Cui, Tiantian Feng, Jiujuan Zhu, Xuanhe Liu, Shiyun Ai
A sensitive and specific photoelectrochemical (PEC) immunosensor was fabricated for subgroup J avian leukosis viruses (ALV-J) analysis based on a dual signal-on strategy. Gold nanoparticles (AuNPs) decorated graphitic carbon nitride (AuNPs/g-C3 N4 ) as photoelectrochemical species and primary antibody (Ab1 ) against ALV-J were immobilized onto ITO electrode in turn. An ALP-CdTe-Ab2 bio-conjugant was fabricated by assembling second antibody (Ab2 ) and alkaline phosphatase (ALP) to CdTe quantum dots (QDs) surface...
October 9, 2018: Biosensors & Bioelectronics
Gaser N Abdelrasoul, Scott MacKay, Saad Salim, Kathleen Ismond, Marcus Tamura, Charfeddine Khalifa, Emma Mannan, Donghai Lin, Tanushree Mandal, Ruth Montgomery, David Wishart, Jie Chen, Rachel Khadaroo
Inadequate blood supply to the intestine can lead to acute mesenteric ischemia (AMI) with a mortality rate ranging from 60% to 90%. This high mortality rate is partially due to late detection and the lack of efficient early diagnostic tests. There is an urgent need for a point-of-care (POC) tool for immediate bedside diagnosis. Here we present for the first time a rapid and non-invasive electrochemical biosensor device based on non-faradic impedance spectroscopy to detect Intestinal Fatty-acid Binding Protein (I-FABP) as an indication of Acute Mesenteric Ischemia...
October 18, 2018: ACS Sensors
Dan Predescu, Shanshan Qin, Monal Patel, Cristina Bardita, Rabia Bhalli, Sanda Predescu
Intersectin-1s (ITSN) deficiency and expression of a biologically active ITSN fragment, result of granzyme B cleavage under inflammatory conditions associated with pulmonary arterial hypertension (PAH), are characteristics of lung tissue of human and animal models of PAH. Recently, we have shown that this ITSN fragment comprising two Epsin15 homology domains (EHITSN ) triggers endothelial cell (EC) proliferation and the plexiform arteriopathy in PAH. Limited evidence also indicates that the EH domains of endocytic proteins such as ITSN, upregulate compensatory endocytic pathways in cells with impaired vesicular trafficking...
2018: Frontiers in Physiology
Peng Zheng, Sujan Kasani, Xiaofei Shi, Ashley E Boryczka, Feng Yang, Haibin Tang, Ming Li, Wanhong Zheng, Daniel E Elswick, Nianqiang Wu
Nutrient pollution is of worldwide environmental and health concerns due to extensive use of nitrogen fertilizers and release of livestock waste, which induces nitrite compounds in aquatic systems. Herein a surface-enhanced Raman scattering (SERS) sensor is developed for nitrite detection based on coupling between the plasmonic gold nanostars and the silver nanopyramid array. When nitrite is present in the assay, an azo group is formed between the 1-naphthylamine-functionalized silver nanopyramids and the 4-aminothiophenol-functionalized gold nanostars...
December 21, 2018: Analytica Chimica Acta
Kumiko Miyajima, Yuki Miwa, Yoshitaka Kitamoto
Porous FePt microcapsules are fabricated for use in bead-based immunoassay technologies, that generally use magnetic spheres to immobilize biomolecules on their surface. The magnetic capsules can be used to carry assay reagents to reduce the time required to perform immunoassay processes, and microsize capsules are easier to manipulate magnetically than nanosize ones. Silica particles of approximately 2.5 μm diameter are used as templates and modified with poly(ethyleneimine) (PEI), which enables FePt nanoparticles to accumulate selectively on the template particles and an FePt shell to be formed by a polyol process...
October 3, 2018: Colloids and Surfaces. B, Biointerfaces
Sunita Kumbhat, Rakhee Gehlot, Kavita Sharma, Urvasini Singh, Vinod Joshi
A surface plasmon resonance (SPR) based immunosensor is presented for highly sensitive and selective detection of 17β-estradiol by the indirect competitive inhibition immuno assay, employing anti-17 β-estradiol antibody as high molecular weight (HMW) interactant. Immobilization of estradiol-BSA conjugate onto the nano thin gold surface was accomplished by covalent amide linkage through self assembled monolayer. The proposed biosensor is simple to fabricate, reproducible and exhibit excellent sensitivity for estrogen (detection limit,1 pg mL-1 ) without any significant interference from structurally similar steroidal hormone, progesterone and non-steroidal compound bisphenol-A...
October 9, 2018: Journal of Pharmaceutical and Biomedical Analysis
Maria Sánchez-Purrà, Biel Roig-Solvas, Cristina Rodriguez-Quijada, Brianna M Leonardo, Kimberly Hamad-Schifferli
We report a quantitative evaluation of the choice of reporters for multiplexed surface-enhanced Raman spectroscopy (SERS). An initial library consisted of 15 reporter molecules that included commonly used Raman dyes, thiolated reporters, and other small molecules. We used a correlation matrix to downselect Raman reporters from the library to choose five candidates: 1,2-bis(4-pyridyl)ethylene, 4-mercaptobenzoic acid, 3,5-dichlorobenzenthiol, pentachlorothiophenol, and 5,5'-dithiobis(2-nitrobenzoic acid). We evaluated the ability to distinguish the five SERS reporters in a dipstick immunoassay for the biomarker human IgG...
September 30, 2018: ACS Omega
Ekta Bansal, Navpreet Kaur, Naveen Mittal
Traditionally small intestinal biopsy has been considered a gold standard for the diagnosis of celiac disease (CD). But now data has shown that serological markers like anti-tissue-transglutaminase antibodies (tTGA) can be used to make the diagnosis with great sensitivity and specificity. The objective of the present study was to evaluate whether patients with high probability of CD and high titre of tTGA, have a high probability of intestinal damage and may not require biopsy for final diagnosis. All the cases with tTGA levels ≥15 IU/ml and who subsequently underwent biopsy from July 2010 to June 2013 were selected...
October 2018: Indian Journal of Clinical Biochemistry: IJCB
Yan Man, Junan Ren, Bingru Li, Xinxin Jin, Ligang Pan
Alternariol monomethyl ether (AME) is one of the major Alternaria mycotoxins present in a wide range of fruits, vegetables, grains, and their products, and possesses the properties of mutagenicity and carcinogenicity. In this study, a simple, rapid, and highly sensitive colorimetric immunosensor based on magnetic nanoparticles (MNPs) was firstly developed for the detection of AME in fruit by nonaggregated gold nanoparticles (GNPs). AME-BSA-Fe3 O4 MNP conjugates and free AME molecules in samples competitively bind with monoclonal antibody (mAb)-GNP conjugates...
October 13, 2018: Analytical and Bioanalytical Chemistry
Hui Lv, Lei Ye, Qian Liu, Song-Guo Li, Tao Li, Na-Li Huang, Yi Gao, Li-Bin Fan, Wei-Dong Du
PURPOSE: Epstein-Barr virus (EBV) is a ubiquitous human gamma herpes virus that infects human epithelial cells and B lymphocytes. EXPERIMENTAL DESIGN: A simple antigen-probed biochip, which was modified with S-S-PEG-COOH and was used as a label-free high-throughput screening method for a combined detection of EBV capsid antigen IgM antibody, capsid antigen IgG antibody and nuclear antigen IgG antibody. RESULTS: This protein biochip had similar feasibility, sensitivity and specificity in comparison with Liaison CLIA...
October 11, 2018: Proteomics. Clinical Applications
Liping Zhu, Desheng Chen, Xiaohui Lu, Yan Qi, Pan He, Chenghui Liu, Zhengping Li
Proteins lack the duplication mechanism like nucleic acids, so the connection of immunoassays with effective nucleic acid amplification techniques has become a powerful way for the detection of trace protein biomarkers in biological fluids. However, such immunoassays generally suffer from rather stringent DNA sequence design and complicated operations. Herein, we propose a simple but highly sensitive flow cytometric immunoassay (FCI) by employing on-bead terminal deoxynucleotidyl transferase (TdT)-initiated template-free DNA extension as an effective signal amplification pathway (TdT-FCI), and gold nanoparticles (AuNPs) co-functionalized with both the detection antibody and a 3'-OH oligonucleotide (ODN) as the transducer to bridge the immunoassay and subsequent TdT-mediated DNA amplification...
August 28, 2018: Chemical Science
Pradeep Neupane, Barbara C Hegarty, Henry S Marr, Ricardo G Maggi, Adam J Birkenheuer, Edward B Breitschwerdt
BACKGROUND: Because of poor sensitivity and questionable specificity of immunofluorescent antibody assays (IFAs), serological diagnosis of Bartonella species infections in dogs remains challenging. Despite limitations, IFA testing is the historical "gold standard" for Bartonella serodiagnosis in animals and humans. Because most diagnostic laboratories test against only 1 or 2 Bartonella spp., testing against a broader panel of Bartonella antigens may enhance diagnostic sensitivity and specificity...
October 11, 2018: Journal of Veterinary Internal Medicine
Takahiro Yamauchi
The mainstay of therapeutic modalities of acute myeloid leukemia (AML) includes intensive chemotherapies and allogeneic hematopoietic cell transplantation. The gold standard of the induction treatment is a regular dose of cytarabine plus anthracycline, and several courses of consolidation therapy are administered. Allogeneic hematopoietic cell transplantation is employed in patients with intermediate-poor risks. No new drugs have been introduced to the treatment of AML for nearly 30 years. However, in 2017, the US Food and Drug Administration approved four novel drugs for treating AML: FLT3 inhibitor midostaurin, IDH2 inhibitor enasidenib, liposomal cytarabine-daunorubicin CPX-351, and revived antibody-drug conjugate gemtuzumab ozogamicin...
2018: [Rinshō Ketsueki] the Japanese Journal of Clinical Hematology
Surender Khurana, Elizabeth M Coyle, Jody Manischewitz, Lisa R King, Jin Gao, Ronald N Germain, Pamela L Schwartzberg, John S Tsang, Hana Golding
Immune responses to inactivated vaccines against avian influenza are poor due in part to lack of immune memory. Adjuvants significantly increased virus neutralizing titers. We performed comprehensive analyses of polyclonal antibody responses following FDA-approved adjuvanted H5N1-A/Indonesia vaccine, administered in presence or absence of AS03. Using Whole Genome Fragment Phage Display Libraries, we observed that AS03 induced antibody epitope diversity to viral hemagglutinin (HA) and neuraminidase compared with unadjuvanted vaccine...
2018: NPJ Vaccines
Panjaporn Chaichana, Kemajittra Jenjaroen, Premjit Amornchai, Suchintana Chumseng, Sayan Langla, Patpong Rongkard, Manutsanun Sumonwiriya, Atthanee Jeeyapant, Narisara Chantratita, Prapit Teparrukkul, Direk Limmathurotsakul, Nicholas P J Day, Vanaporn Wuthiekanun, Susanna J Dunachie
Melioidosis is a major neglected tropical disease with high mortality, caused by the Gram-negative bacterium Burkholderia pseudomallei ( Bp ). Microbiological culture remains the gold standard for diagnosis, but a simpler and more readily available test such as an antibody assay is highly desirable. In this study, we conducted a serological survey of blood donors ( n = 1,060) and adult melioidosis patients ( n = 200) in northeast Thailand to measure the antibody response to Bp using the indirect hemagglutination assay (IHA)...
October 8, 2018: American Journal of Tropical Medicine and Hygiene
Jin-Yuan Wu, Yan Zhou, Guang-Ming Zhang, Guo-Fa Mu, Shan Yi, Na Yin, Yu-Ping Xie, Xiao-Chen Lin, Hong-Jun Li, Mao-Sheng Sun
AIM: To determine the distribution of rotavirus VP7 gene in hospitalized children in Yunnan, China. METHODS: A total of 366 stool specimens were collected from hospitalized children in hospitals in Yunnan Province from September 2010 to December 2013. The genomic RNA electropherotypes and the G genotypes of the rotaviruses were determined. A phylogenetic analysis of the VP7 gene was performed. Rotavirus isolation was performed, and characterized by plaque, minimum essential medium, and all genes sequence analysis...
October 6, 2018: World Journal of Clinical Cases
Nadia El Alami El Hassani, Abdoullatif Baraket, Selim Boudjaoui, Ernandes Taveira Tenório Neto, Joan Bausells, Nezha El Bari, Benachir Bouchikhi, Abdelhamid Elaissari, Abdelhamid Errachid, Nadia Zine
Tetracycline (TC) is a veterinary drug, wildly prescribed for prophylactic and therapeutic purposes. Consequently, its remaining residues in food products have to be regularized. We report in this paper about the development of a novel immunosensor based on an integrated bio micro-electromechanical system (Bio-MEMS) containing eight gold microelectrodes (µWEs), an integrated silver and platinum reference and counter electrodes, respectively. TC immobilization on the µWEs surface was conducted using three methods...
September 13, 2018: Biosensors & Bioelectronics
Ariel M Wilson, Javier Mazzaferri, Eric Bergeron, Sergiy Patskovsky, Paule Marcoux-Valiquette, Santiago Costantino, Przemyslaw Mike Sapieha, Michel Meunier
Vision loss caused by retinal diseases affects hundreds of millions of individuals worldwide. The retina is a delicate central nervous system tissue stratified into layers of cells with distinct roles. Currently, there is a void in treatments that selectively target diseased retinal cells and current therapeutic paradigms present complications associated with off-target effects. Herein, as a proof-of-concept, we introduce an in vivo method using a femtosecond laser to locally optoporate retinal ganglion cells (RGCs) targeted with functionalized gold nanoparticles (AuNPs)...
October 4, 2018: Nano Letters
Dong-Ping Zhang, Ling-E Wang, Xiao-Ying Liu, Zhi-Hua Luo, Lei Zheng, Yun He, Bo Zhang
A facile and feasible photoelectrochemical (PEC) immunoassay based on plasmon-enhanced energy transfer between gold nanoparticles (AuNPs) and CdS quantum dots (QDs)/g-C3 N4 nanosheets was developed for the ultrasensitive detection of lipoprotein-associated phospholipase A2 (Lp-PLA2 ). To construct such a sensing platform, the immunosensor was prepared by immobilizing Lp-PLA2 on a CdS QDs/g-C3 N4 -modified electrode. A competitive-type immunoreaction was utilized for Lp-PLA2 detection, with AuNP-labeled anti-Lp-PLA2 antibody used as the competitor...
October 3, 2018: Analytical and Bioanalytical Chemistry
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"