keyword
MENU ▼
Read by QxMD icon Read
search

Affinity t cell

keyword
https://www.readbyqxmd.com/read/28930660/the-irf4-gene-regulatory-module-functions-as-a-read-write-integrator-to-dynamically-coordinate-t%C3%A2-helper-cell-fate
#1
Veena Krishnamoorthy, Sunil Kannanganat, Mark Maienschein-Cline, Sarah L Cook, Jianjun Chen, Neil Bahroos, Evelyn Sievert, Emily Corse, Anita Chong, Roger Sciammas
Transcriptional regulation during CD4(+) T cell fate decisions enables their differentiation into distinct states, guiding immune responses toward antibody production via Tfh cells or inflammation by Teff cells. Tfh-Teff cell fate commitment is regulated by mutual antagonism between the transcription factors Bcl6 and Blimp-1. Here we examined how T cell receptor (TCR) signals establish and arbitrate Bcl6-Blimp-1 counter-antagonism. We found that the TCR-signal-induced transcription factor Irf4 is essential for the differentiation of Bcl6-expressing Tfh and Blimp-1-expressing Teff cells...
September 19, 2017: Immunity
https://www.readbyqxmd.com/read/28923586/genetic-code-asymmetry-supports-diversity-through-experimentation-with-posttranslational-modifications
#2
REVIEW
James W Dennis
Protein N-glycosylation has been identified in all three domains of life presumably conserved for its early role in glycoprotein folding. However, the N-glycans added to proteins in the secretory pathway of multicellular organisms are remodeling in the Golgi, increasing structural diversity exponentially and adding new layers of functionality in immunity, metabolism and other systems. The branching and elongation of N-glycan chains found on cell surface receptors generates a gradation of affinities for carbohydrate-binding proteins, the galectin, selectin and siglec families...
September 15, 2017: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/28923066/data-integration-from-pathology-slides-for-quantitative-imaging-of-multiple-cell-types-within-the-tumor-immune-cell-infiltrate
#3
Zhaoxuan Ma, Stephen L Shiao, Emi J Yoshida, Steven Swartwood, Fangjin Huang, Michael E Doche, Alice P Chung, Beatrice S Knudsen, Arkadiusz Gertych
BACKGROUND: Immune cell infiltrates (ICI) of tumors are scored by pathologists around tumor glands. To obtain a better understanding of the immune infiltrate, individual immune cell types, their activation states and location relative to tumor cells need to be determined. This process requires precise identification of the tumor area and enumeration of immune cell subtypes separately in the stroma and inside tumor nests. Such measurements can be accomplished by a multiplex format using immunohistochemistry (IHC)...
September 18, 2017: Diagnostic Pathology
https://www.readbyqxmd.com/read/28922396/biochemical-and-immunological-characterization-of-a-novel-monoclonal-antibody-against-mouse-leukotriene-b4-receptor-1
#4
Fumiyuki Sasaki, Tomoaki Koga, Kazuko Saeki, Toshiaki Okuno, Saiko Kazuno, Tsutomu Fujimura, Yasuyuki Ohkawa, Takehiko Yokomizo
Leukotriene B4 (LTB4) receptor 1 (BLT1) is a G protein-coupled receptor expressed in various leukocyte subsets; however, the precise expression of mouse BLT1 (mBLT1) has not been reported because a mBLT1 monoclonal antibody (mAb) has not been available. In this study, we present the successful establishment of a hybridoma cell line (clone 7A8) that produces a high-affinity mAb for mBLT1 by direct immunization of BLT1-deficient mice with mBLT1-overexpressing cells. The specificity of clone 7A8 was confirmed using mBLT1-overexpressing cells and mouse peripheral blood leukocytes that endogenously express BLT1...
2017: PloS One
https://www.readbyqxmd.com/read/28920671/development-of-a-multifunctional-nanobiointerface-based-on-self-assembled-fusion-protein-rsbpa-zz-for-blood-cell-enrichment-and-phenotyping
#5
Mario Rothbauer, Martin Frauenlob, Karoline Gutkas, Michael B Fischer, Eva Kathrin Sinner, Seta Küpcü, Peter Ertl
We present a multifunctional nanobiointerface for blood cell capture and phenotyping applications that features both excellent antifouling properties and high antibody activity. Multifunctionality is accomplished by modifying polymeric materials using self-assembled S-layer fusion-protein rSbpA/ZZ to immobilize high density antibodies at the two protein A binding sites of the rSbpA/ZZ nanolattice structure. Controlled orientation and alignment of the antibodies reduced antibody consumption 100-fold and increased cell capture efficiency 4-fold over standard methodologies...
September 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28920599/magnetic-nanoparticle-enhanced-surface-plasmon-resonance-biosensor-for-extracellular-vesicle-analysis
#6
Agnes T Reiner, Nicolas-Guillermo Ferrer, Priyamvada Venugopalan, Ruenn Chai Lai, Sai Kiang Lim, Jakub Dostálek
The sensitive analysis of small lipid extracellular vesicles (EVs) by using a grating-coupled surface plasmon resonance (GC-SPR) biosensor has been reported. In order to enable the analysis of trace amounts of EVs present in complex liquid samples, the target analyte is pre-concentrated on the sensor surface by using magnetic nanoparticles and its affinity binding is probed by wavelength interrogation of SPR. The GC-SPR has been demonstrated to allow for the implementation of efficient pulling of EVs to the sensor surface by using magnetic nanoparticles and an external magnetic field gradient applied through the sensor chip...
September 18, 2017: Analyst
https://www.readbyqxmd.com/read/28919450/seasonal-expression-of-p450c17-and-5%C3%AE-reductase-2-in-the-scented-gland-of-male-muskrats-ondatra-zibethicus
#7
Wentao Han, Wenqian Xie, Yan Zhang, Fengwei Zhang, Haolin Zhang, Yingying Han, Zhengrong Yuan, Qiang Weng
Cytochrome P450 17A1 (P450c17) is the key enzyme required for the production of androgenic sex steroids by converting progestogens to androgens. 5α-reductases are enzymes that convert testosterone (T) to dihydrotestosterone (DHT), which has a greater affinity for androgen receptors (AR) and stronger action than T. Our previous studies revealed that the scented glands of male muskrats expressed AR during the breeding and nonbreeding seasons. To further seek evidence of the activities of androgens in scented glands, the expression patterns of P450c17 and 5α-reductase 2 were investigated in the scented glands of male muskrats during the breeding and nonbreeding seasons...
September 14, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28918052/a-ctla-4-antagonizing-dna-aptamer-with-antitumor-effect
#8
Bo-Tsang Huang, Wei-Yun Lai, Yi-Chung Chang, Jen-Wei Wang, Shauh-Der Yeh, Emily Pei-Ying Lin, Pan-Chyr Yang
The successful translation of cytotoxic T lymphocyte antigen-4 (CTLA-4) blockade has revolutionized the concept of cancer immunotherapy. Although monoclonal antibody therapeutics remain the mainstream in clinical practice, aptamers are synthetic oligonucleotides that encompass antibody-mimicking functions. Here, we report a novel high-affinity CTLA-4-antagonizing DNA aptamer (dissociation constant, 11.84 nM), aptCTLA-4, which was identified by cell-based SELEX and high-throughput sequencing. aptCTLA-4 is relatively stable in serum, promotes lymphocyte proliferation, and inhibits tumor growth in cell and animal models...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28916524/new-methods-to-analyze-b-cell-immune-responses-to-thymus-dependent-antigen-sheep-red-blood-cells
#9
Ellen J McAllister, John R Apgar, Charlotte R Leung, Robert C Rickert, Julia Jellusova
B cells contribute critically to an effective immune response by producing Ag-specific Abs. During the immune response to so-called "thymus-dependent Ags," activated B cells seek T cell help and form germinal centers. In contrast, thymus-independent Ags generally do not induce germinal center formation. In the germinal center, B cells undergo somatic hypermutation, affinity-based clonal expansion, and differentiation to produce plasma cells and memory B cells. Valuable insight into these processes has been gained by using model hapten-carrier complexes or SRBCs...
September 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28914198/pemtb-a-database-of-mhc-antigenic-peptide-of-mycobacterium-tuberculosis
#10
Qamar Zia, Asim Azhar, Shadab Ahmad, Mohammad Afsar, Ziaul Hasan, Mohammad Owais, Mahfooz Alam, Shabab Akbar, Magdah Ganash, Ghulam Md Ashraf, Swaleha Zubair, Gjumrakch Aliev
BACKGROUND: For design of a subunit vaccine for tuberculosis, identification of antigenic T-cell epitope is of utmost importance. Several MHC prediction server are available that can accurately predict antigenic peptide of variable lengths. However, peptides predicted from one server not necessarily are predicted form another server, thus creating a confusing situation for scientists to choose a best epitope. METHOD: Keeping the above problem in mind, we developed a comprehensive database of peptides of Mycobacterial proteins...
September 14, 2017: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28912108/leptospira-borgpetersenii-hybrid-leucine-rich-repeat-protein-cloning-and-expression-immunogenic-identification-and-molecular-docking-evaluation
#11
Tepyuda Sritrakul, Supachai Nitipan, Worawidh Wajjwalku, Anchalee La-Ard, Chattip Suphatpahirapol, Wimol Petkarnjanapong, Boonsong Ongphiphadhanakul, Siriwan Prapong
Leptospirosis is an important zoonotic disease, and the major outbreak of this disease in Thailand in 1999 was due largely to the Leptospira borgpetersenii serovar Sejroe. Identification of the leucine-rich repeat (LRR) LBJ_2271 protein containing immunogenic epitopes and the discovery of the LBJ_2271 ortholog in Leptospira serovar Sejroe, KU_Sej_R21_2271, led to further studies of the antigenic immune properties of KU_Sej_LRR_2271. The recombinant hybrid (rh) protein was created and expressed from a hybrid PCR fragment of KU_Sej_R21_2271 fused with DNA encoding the LBJ_2271 signal sequence for targeting protein as a membrane-anchoring protein...
September 11, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28911105/a-novel-nucleoid-associated-protein-coordinates-chromosome-replication-and-chromosome-partition
#12
James A Taylor, Gaël Panis, Patrick H Viollier, Gregory T Marczynski
We searched for regulators of chromosome replication in the cell cycle model Caulobacter crescentus and found a novel DNA-binding protein (GapR) that selectively aids the initiation of chromosome replication and the initial steps of chromosome partitioning. The protein binds the chromosome origin of replication (Cori) and has higher-affinity binding to mutated Cori-DNA that increases Cori-plasmid replication in vivo. gapR gene expression is essential for normal rapid growth and sufficient GapR levels are required for the correct timing of chromosome replication...
September 6, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28900679/ctla-4-an-essential-immune-checkpoint-for-t-cell-activation
#13
Shunsuke Chikuma
The response of peripheral T lymphocytes (T cell) is controlled by multiple checkpoints to avoid unwanted activation against self-tissues. Two opposing costimulatory receptors, CD28 and CTLA-4, on T cells bind to the same ligands (CD80 and CD86) on antigen-presenting cells (APCs), and provide positive and negative feedback for T-cell activation, respectively. Early studies suggested that CTLA-4 is induced on activated T cells and binds to CD80/CD86 with much stronger affinity than CD28, providing a competitive inhibition...
September 13, 2017: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/28900678/socs1-regulator-of-t-cells-in-autoimmunity-and-cancer
#14
Subburaj Ilangumaran, Diwakar Bobbala, Sheela Ramanathan
SOCS1 is a negative feedback regulator of cytokine and growth factor receptor signaling, and plays an indispensable role in attenuating interferon gamma signaling. Studies on SOCS1-deficient mice have established a crucial role for SOCS1 in regulating CD8(+) T cell homeostasis. In the thymus, SOCS1 prevents thymocytes that had failed positive selection from surviving and expanding, ensures negative selection and prevents inappropriate developmental skewing toward the CD8 lineage. In the periphery, SOCS1 not only controls production of T cell stimulatory cytokines but also attenuates the sensitivity of CD8(+) T cells to synergistic cytokine stimulation and antigen non-specific activation...
September 13, 2017: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/28900667/ammonium-trichloro-1-2-ethanediolato-o-o-tellurate-cures-experimental-visceral-leishmaniasis-by-redox-modulation-of-leishmania-donovani-trypanothione-reductase-and-inhibiting-host-integrin-linked-pi3k-akt-pathway
#15
Preeti Vishwakarma, Naveen Parmar, Pragya Chandrakar, Tanuj Sharma, Manoj Kathuria, Pramod K Agnihotri, Mohammad Imran Siddiqi, Kalyan Mitra, Susanta Kar
In an endeavor to search for affordable and safer therapeutics against debilitating visceral leishmaniasis, we examined antileishmanial potential of ammonium trichloro [1,2-ethanediolato-O,O']-tellurate (AS101); a tellurium based non toxic immunomodulator. AS101 showed significant in vitro efficacy against both Leishmania donovani promastigotes and amastigotes at sub-micromolar concentrations. AS101 could also completely eliminate organ parasite load from L. donovani infected Balb/c mice along with significant efficacy against infected hamsters (˃93% inhibition)...
September 12, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28900542/mitochondrial-dna-haplotypes-induce-differential-patterns-of-dna-methylation-that-result-in-differential-chromosomal-gene-expression-patterns
#16
William T Lee, Xin Sun, Te-Sha Tsai, Jacqueline L Johnson, Jodee A Gould, Daniel J Garama, Daniel J Gough, Matthew McKenzie, Ian A Trounce, Justin C St John
Mitochondrial DNA copy number is strictly regulated during development as naive cells differentiate into mature cells to ensure that specific cell types have sufficient copies of mitochondrial DNA to perform their specialised functions. Mitochondrial DNA haplotypes are defined as specific regions of mitochondrial DNA that cluster with other mitochondrial sequences to show the phylogenetic origins of maternal lineages. Mitochondrial DNA haplotypes are associated with a range of phenotypes and disease. To understand how mitochondrial DNA haplotypes induce these characteristics, we used four embryonic stem cell lines that have the same set of chromosomes but possess different mitochondrial DNA haplotypes...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28900157/synergy-of-two-low-affinity-nlss-determines-the-high-avidity-of-influenza-a-virus-nucleoprotein-np-for-human-importin-%C3%AE-isoforms
#17
Wei Wu, Rajeshwer S Sankhala, Tyler J Florio, Lixin Zhou, Nhan L T Nguyen, Ravi K Lokareddy, Gino Cingolani, Nelly Panté
The influenza A virus nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs from all strains of influenza A viruses contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. Through site-directed mutagenesis and functional analysis, we found that NLS2 is also monopartite and is indispensable for viral infection. Atomic structures of importin α bound to two variants of NLS2 revealed NLS2 primarily binds the major-NLS binding site of importin α, unlike NLS1 that associates with the minor NLS-pocket...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28894200/soluble-hla-associated-peptide-from-psf1-has-a-cancer-vaccine-potency
#18
Mari Yoshida, Yukichi Ishioka, Takamasa Ozawa, Hirohisa Okuyama, Motofumi Iguchi, Takeshi Ota, Takaomi Ito, Morio Nagira, Atsushi Morita, Hidekazu Tanaka, Hisamichi Naito, Hiroyasu Kidoya, Nobuyuki Takakura
Partner of sld five 1 (PSF1) is an evolutionary conserved DNA replication factor involved in DNA replication in lower species, which is strongly expressed in normal stem cell populations and progenitor cell populations. Recently, we have investigated PSF1 functions in cancer cells and found that PSF1 plays a significant role in tumour growth. These findings provide initial evidence for the potential of PSF1 as a therapeutic target. Here, we reveal that PSF1 contains an immunogenic epitope suitable for an antitumour vaccine...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28892606/crispr-mediated-tagging-of-endogenous-proteins-with-a-luminescent-peptide
#19
Marie K Schwinn, Thomas Machleidt, Kris Zimmerman, Christopher T Eggers, Andrew S Dixon, Robin Hurst, Mary P Hall, Lance P Encell, Brock F Binkowski, Keith V Wood
Intracellular signaling pathways are mediated by changes in protein abundance and post-translational modifications. A common approach for investigating intracellular signaling and the effects induced by synthetic compounds is to overexpress recombinant reporter genes within a cell. Genome editing with CRISPR/Cas9 offers a means to better preserve native biology by appending reporters directly onto the endogenous genes. An optimal reporter for this purpose would be small to negligibly influence intracellular processes, be readily linked to the endogenous genes with minimal experimental effort, and be sensitive enough to detect low expressing proteins...
September 11, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28890387/peptides-derived-from-transcription-factor-eb-bind-to-calcineurin-at-a-similar-region-as-the-nfat-type-motif
#20
Ruiwen Song, Jing Li, Jin Zhang, Lu Wang, Li Tong, Ping Wang, Huan Yang, Qun Wei, Huaibin Cai, Jing Luo
Calcineurin (CN) is involved in many physiological processes and interacts with multiple substrates. Most of the substrates contain similar motifs recognized by CN. Recent studies revealed a new CN substrate, transcription factor EB (TFEB), which is involved in autophagy. We showed that a 15-mer QSYLENPTSYHLQQS peptide from TFEB (TFEB-YLENP) bound to CN. When the TFEB-YLENP peptide was changed to YLAVP, its affinity for CN increased and it had stronger CN inhibitory activity. Molecular dynamics simulations revealed that the TFEB-YLENP peptide has the same docking sites in CN as the 15-mer DQYLAVPQHPYQWAK motif of the nuclear factor of activated T cells, cytoplasmic 1 (NFATc1-YLAVP)...
September 7, 2017: Biochimie
keyword
keyword
16358
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"