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Autologous Fat Transplantation

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https://www.readbyqxmd.com/read/30516559/influence-of-recipient-site-on-the-function-and-survival-of-fat-grafts
#1
Xi Chen, Yaohao Wu, Guangpeng Liu
BACKGROUND: Autologous fat grafting has become an increasingly common procedure for soft tissue augmentation throughout the body. However, the long-term outcome is always unpredictable because of inconsistent graft survival. Based on the "law of use and disuse," we speculate that the volume loss of fat grafts will occur when transferred into a site where there is less fat. The purpose of this study is to investigate the cause of high resorption rate from the perspective of fat function after transplantation...
December 3, 2018: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/30511971/management-of-fat-necrosis-after-autologous-fat-transplantation-for-breast-augmentation
#2
Jeng-Yee Lin, Ping Song, Lee L Q Pu
BACKGROUND: Palpable lumps as sequelae of fat necrosis after fat grafting to the breast may not only cause physical or psychological symptoms to patients but also potentially complicate breast cancer screening. In this article, the authors present their experience in management of fat necrosis following fat grafting for breast augmentation. METHODS: Over a 5-year period, a total of 685 Asian women (age range, 20 to 58 years) underwent autologous fat transplantation to the breasts...
November 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/30482066/adipose-tissue-transplant-in-recurrent-folliculitis-decalvans
#3
Marinella Tedesco
Folliculitis decalvans is a rare clinical disorder classified as primary neutrophilic scarring alopecia with a slight preference for the male gender. Here, we report the use of autologous fat transplantation as a source of stem cell therapy for hair re-growth assisted by inflammatory action of the fat itself in a female patient. The patient underwent adipose transplantation in April and September 2017. After treatments, the patient had no new pustules and no longer had pain or burning sensation in the affected area...
March 2018: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/30460739/the-use-of-fat-grafting-and-platelet-rich-plasma-for-wound-healing-a-review-of-the-current-evidence
#4
Oliver J Smith, Gavin Jell, Ash Mosahebi
Fat grafting is becoming a common procedure in regenerative medicine because of its high content of growth factors and adipose derived stem cells (ADSCs) and the ease of harvest, safety, and low cost. The high concentration of ADSCs found in fat has the potential to differentiate into a wide range of wound-healing cells including fibroblasts and keratinocytes as well as demonstrating proangiogenic qualities. This suggests that fat could play an important role in wound healing. However retention rates of fat grafts are highly variable due in part to inconsistent vascularisation of the transplanted fat...
November 20, 2018: International Wound Journal
https://www.readbyqxmd.com/read/30442054/aesthetic-improvements-of-radial-forearm-flap-donor-site-by-autologous-fat-transplantation
#5
Benedetto Longo, Michail Sorotos, Rosaria Laporta, Fabio Santanelli di Pompeo
Despite its reliability, radial forearm (RF) flap is still affected by high donor site morbidity with poor cosmetic and functional outcomes after coverage with skin grafts. Having fat grafting demonstrated promising and effective filling and rejuvenating properties, we considered and tested it as a valuable alternative to dermal substitutes for the aesthetic improvement of RF flap donor site. Thirty-three patients with previous RF free flap reconstruction and poor donor site outcomes after full-thickness skin grafting to RF region were evaluated for secondary fat injection to improve outcomes...
November 16, 2018: Journal of Plastic Surgery and Hand Surgery
https://www.readbyqxmd.com/read/30431135/in-vitro-enhancement-and-functional-characterization-of-neurite-outgrowth-by-undifferentiated-adipose-derived-stem-cells
#6
Vesna Bucan, Malte Fliess, Reinhild Schnabel, Claas-Tido Peck, Desiree Vaslaitis, Angela Fülbier, Kerstin Reimers, Sarah Strauss, Peter M Vogt, Christine Radtke
Adipose‑derived stem cells (ASCs) can easily be obtained and expanded in vitro for use in autologous cell therapy. Via their production of cytokines and neurotrophic factors, transplanted ASCs provide neuroprotection, neovascularization and induction of axonal sprouting. However, the influencing mechanism of undifferentiated ASCs on nerve regeneration is currently only partially understood. In the present study, undifferentiated ASCs and cutaneous primary afferent dorsal root ganglion (DRG) neurons were co‑cultured in order to investigate their interaction...
January 2019: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/30404192/induction-of-expandable-adipose-derived-mesenchymal-stem-cells-from-aged-mesenchymal-stem-cells-by-a-synthetic-self-replicating-rna
#7
Chika Miyagi-Shiohira, Yoshiki Nakashima, Naoya Kobayashi, Shinji Kitamura, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi
Adipose-derived mesenchymal stem cells (ADSCs) have attracted attention due to their potential for use in the treatment of various diseases. However, the self-renewal capacity of ADSCs is restricted and their function diminishes during passage. We previously generated induced tissue-specific stem cells from mouse pancreatic cells using a single synthetic self-replicating Venezuelan Equine Encephalitis (VEE)-reprogramming factor (RF) RNA replicon (SR-RNA) expressing the reprogramming factors POU class 5 homeobox 1 (OCT4), Krueppel-like factor 4 (KLF4), Sex determining region Y-box 2 (SOX2), and Glis Family Zinc Finger 1 (GLIS1)...
November 6, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30382375/impact-of-human-mesenchymal-cells-of-different-body-site-origins-on-the-maturation-of-dermo-epidermal-skin-substitutes
#8
Katarzyna Michalak-Micka, Agnes S Klar, Sophie Böttcher-Haberzeth, Ernst Reichmann, Martin Meuli, Thomas Biedermann
AIM OF THE STUDY: The use of autologous bio-engineered dermo-epidermal skin substitutes (DESS) yields a pivotal opportunity to cover large skin defects in human patients. These skin grafts consist of both epidermal and dermal compartments necessary for robust and permanent functional wound closure. In this study, we investigated the impact of mesenchymal cells derived from different body site origins on the expression pattern of diverse markers within DESS. METHODS: Human keratinocytes were obtained from interfollicular epidermis, and mesenchymal cells were isolated from foreskin, palmar skin, fat tissue, and tonsils...
October 31, 2018: Pediatric Surgery International
https://www.readbyqxmd.com/read/30369133/-reconsideration-of-burn-scar-treatment
#9
J N Huan
Since the mechanisms of scar formation are not fully understood, at least to date, there is no ideal method to prevent and treat scar after burn. In recent years, on the basis of conventional treatments, such as pressure therapy, external use of silicone gel, and intralesional injections of corticosteroids, the therapeutic approaches of fractional carbon dioxide laser treatment, injection of anti-vascular endothelial growth factor antibody, autologous fat transplantation, injection of botulinum toxin A, radiation therapy etc...
October 20, 2018: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/30345577/persistent-damage-on-magnetic-resonance-enterography-in-patients-with-crohn-s-disease-in-endoscopic-remission
#10
Jordi Rimola, Ignacio Alfaro, Agnès Fernández-Clotet, Jesús Castro-Poceiro, Daniel Vas, Sonia Rodríguez, Maria Carme Masamunt, Ingrid Ordás, Elena Ricart, Julián Panés
BACKGROUND: In Crohn's disease, it is essential to distinguish between persistent damage and abnormalities that can heal with anti-inflammatory therapy. AIM: To magnetic resonance enterography (MRE) lesions that persist in patients in long-standing endoscopic remission, analyse their relationship with baseline characteristics, and determine their prognostic implications. METHODS: We systematically reviewed pre- and post-treatment MRE findings in patients with Crohn's disease and severe inflammation (segmental CDEIS ≥ 7 or ulcers in at least one segment) who achieved endoscopic remission (CDEIS < 2) after 1 year of treatment with TNF-inhibitors or autologous haematopoietic stem-cell transplantation...
October 22, 2018: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/30326627/18-f-fdg-pet-ct-in-extensive-graft-versus-host-disease-of-the-gastrointestinal-tract-following-autologous-stem-cell-transplantation
#11
Danijela Dejanovic, Annemarie Amtoft, Annika Loft
Graft-versus-host-disease (GVHD) following stem cell transplantation (SCT) is a common complication in patients that have undergone allogenic SCT but rare in recipients of autologous SCT. Gastro-intestinal tract (GIT)-GVHD can be difficult to diagnose due to non-specific symptoms such as fever, nausea, diarrhea, and vomiting; a histological confirmation is therefore required. Here, we present the findings of a whole-body 18 FDG PET/CT with extensive and multifocal involvement of the GIT in a patient that developed severe acute GVHD 93 days post autologous SCT for Hodgkin's lymphoma...
October 15, 2018: Diagnostics
https://www.readbyqxmd.com/read/30271860/autologous-and-heterotopic-transplantation-of-adipose-stromal-vascular-fraction-ameliorates-stress-urinary-incontinence-in-rats-with-simulated-childbirth-trauma
#12
Ken-Ichi Inoue, Satoko Kishimoto, Kanya Kaga, Miki Fuse, Akira Furuta, Tomonori Yamanishi
Introduction: Autologous transplantation of adipose stromal vascular fraction (SVF) is a cost-effective and technically accessible option for cell therapy. Clinical study of SVF transplantation for male stress urinary incontinence (SUI) is underway, but the effectiveness remains unknown for female SUI, majority of which is caused by childbirth trauma. Methods: Vaginal Distension (VD) rats were generated as in vivo model for female SUI. To quantitate the severity of SUI, leak point pressure (LPP) was measured by placing a bladder catheter...
June 2018: Regenerative Therapy
https://www.readbyqxmd.com/read/30271849/autologous-adipose-derived-stem-cell-sheets-enhance-the-strength-of-intestinal-anastomosis
#13
Yasuhiro Maruya, Nobuo Kanai, Shinichiro Kobayashi, Kurodo Koshino, Teruo Okano, Susumu Eguchi, Masayuki Yamato
Objective: Adipose-derived stem cells (ASCs) are capable of multiple differentiation pathways, imparting immunomodulatory effects, and secreting factors that are important for wound healing. These characteristics can be exploited to decrease the incidence of anastomotic leakage. Methods: In order to delay local wound healing at the anastomotic site, we induced ischemia in a portion of porcine small intestine by ligating vessels. Then, we injected mitomycin C into the serosa of the small intestine above the ligated vessels...
December 2017: Regenerative Therapy
https://www.readbyqxmd.com/read/30251158/influence-of-preadipocyte-conditioned-medium-on-the-proliferation-and-invasive-potential-of-breast-cancer-cell-lines-in-vitro
#14
Anna Jablonka, Jan Scheich, Frank Jacobsen, Tobias Hirsch, Mazen Hagouan, Marcus Lehnhardt, Clemens B Tempfer, Günther A Rezniczek
PURPOSE: Autologous transplantation of adipose tissue into the breast is commonly performed in clinical practice, but its oncological safety has not been established. METHODS: We conducted an in vitro study to assess the influence of factors released by adipose-derived stem cells (ASCs), from multiple source tissues and harvested using different techniques, on proliferation and invasiveness of two breast cancer cell lines. RESULTS: Fat specimens of 66 donors (57 female, 9 male) were collected and 44 ASC cultures were established...
September 24, 2018: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/30238985/stromal-vascular-fraction-cells-plus-sustained-release-vegf-ang-1-plga-microspheres-improve-fat-graft-survival-in-mice
#15
Yucang He, Xiaofang Yu, Zhuojie Chen, Liqun Li
Autologous fat transplantation is increasingly applied in plastic and reconstructive surgery. Stromal vascular fraction cells (SVFs) combined with angiogenic factors, such as VEGF (vascular endothelial growth factor A) and Ang-1 (angiogenin-1), can improve angiogenesis, which is a critical factor for graft survival. However, direct transplant with such a mixture is insufficient owing to the short half-life of angiogenic factors. In this study, we evaluated whether a double sustained release system of VEGF/ANG-1-PLGA (poly (lactic-co-glycolic acid)) microspheres plus SVFs can improve angiogenesis and graft survival after autologous fat transplantation...
September 21, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30236223/autologous-fat-transplantation
#16
Christian Herold, Hans-Oliver Rennekampff, Sixtus Allert
No abstract text is available yet for this article.
September 3, 2018: Deutsches Ärzteblatt International
https://www.readbyqxmd.com/read/30100221/emerging-aspects-of-the-body-composition-bone-marrow-adipose-tissue-and-skeletal-phenotypes-in-type-1-diabetes-mellitus
#17
Adriana L Carvalho, Bianca Massaro, Luciana T P E Silva, Carlos E G Salmon, Sandra Y Fukada, Marcello H Nogueira-Barbosa, Jorge Elias, Maria C F Freitas, Carlos E B Couri, Maria C Oliveira, Belinda P Simões, Clifford J Rosen, Francisco J A de Paula
Anthropomorphic measures among type 1 diabetic patients are changing as the obesity epidemic continues. Excess fat mass may impact bone density and ultimately fracture risk. We studied the interaction between bone and adipose tissue in type 1 diabetes subjects submitted to two different clinical managements: (I) conventional insulin therapy or (II) autologous nonmyeloablative hematopoietic stem-cell transplantation (AHST). The study comprised 3 groups matched by age, gender, height and weight: control (C = 24), type 1 diabetes (T1D = 23) and type 1 diabetes treated with AHST (T1D-AHST = 9)...
July 5, 2018: Journal of Clinical Densitometry
https://www.readbyqxmd.com/read/30097407/autologous-dermis-fat-grafts-in-head-and-neck-patients-indications-and-evaluation-in-reconstructive-surgery
#18
Sven Holger Baum, Christopher Mohr
PURPOSE: The aim of this study was to examine the indications and results of autologous dermis-fat grafts in the reconstruction of maxillofacial soft-tissue defects. MATERIALS AND METHODS: A total of 93 patients with dermis-fat graft reconstruction due to a soft tissue defect in the head and neck region were enrolled in this retrospective clinical study between March 2002 and January 2017. They were classified into the subgroups 'parotid surgery', 'orbital surgery', and 'facial surgery'...
July 24, 2018: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/30067529/lipofilling-of-fingertips-for-volume-defects-after-fingertip-injuries
#19
Michael K Cerny, Philipp Moog, Anna-Theresa Bauer, Daniel Schmauss, Laszlo Varga, Matthias M Aitzetmueller, Hans-Günther Machens, Dominik Duscher, Holger Erne
INTRODUCTION: Regardless of the good outcomes of conservatively treated fingertip injuries, many patients complain about volume defects and hypoesthesia. Injection of autologous fat into the defect zone may resolve the volume problem and stimulate digital nerve regeneration by adipose derived stem cell transplantation. METHODS: We analyzed 5 volume defects resulting from conservatively treated fingertip injuries in 4 patients (male to female ratio, 2:2), 6 months after the injection of autologous abdominal fat into the defect zone retrospectively...
December 2018: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/30058570/safety-and-efficacy-of-autologous-noncultured-dermal-cell-suspension-transplantation-in-the-treatment-of-localized-facial-volume-loss-a-pilot-study
#20
Alok Kumar Sahoo, Savita Yadav, Vinod K Sharma, Anita Singh Parihar, Surabhi Vyas, Somesh Gupta
Background: Available options for correction of facial volume loss, such as synthetic fillers, autologous fat and cultured fibroblasts, have limitations viz. temporary effect and high cost. Aim: To assess the use of a novel technique, autologous non-cultured dermal cell suspension transplantation, for correction of localized facial volume loss due to inflammatory pathologies. Methods: It was a pilot study conducted in the Dermatology Outpatient Department, All India Institute of Medical Sciences (AIIMS), New Delhi, India...
July 30, 2018: Indian Journal of Dermatology, Venereology and Leprology
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