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Epinephelus immunity

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https://www.readbyqxmd.com/read/30061893/integration-of-rnai-and-rna-seq-reveals-the-immune-responses-of-epinephelus-coioides-to-sigx-gene-of-pseudomonas-plecoglossicida
#1
Yujia Sun, Gang Luo, Lingmin Zhao, Lixing Huang, Yingxue Qin, Yongquan Su, Qingpi Yan
Pseudomonas plecoglossicida is an important pathogen for aquaculture and causes high mortality in various marine fishes. Expression of sigX was found significantly up-regulated at 18°C than at 28°C, which was verified by quantitative real-time PCR (qRT-PCR). RNAi significantly reduced the content of sigX mRNA of P. plecoglossicida , whether in in vitro or in the spleen at all sampling time points. Compared with the wild-type strain, the infection of sigX -RNAi strain resulted in the onset time delay, and 20% reduction in mortality of Epinephelus coioides , as well as alleviates in the symptoms of E...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/30055251/identification-and-functional-analysis-of-grouper-epinephelus-coioides-b-cell-linker-protein-blnk
#2
Ze-Quan Mo, Jiu-Le Wang, Rui Han, Qing Han, Yan-Wei Li, Hong-Yan Sun, Xiao-Chun Luo, Xue-Ming Dan
B-cell linker protein (BLNK) is an adaptor protein that plays a crucial role in the B cell antigen receptor (BCR) signal pathway. To investigate the function of BLNK in teleost fish, we cloned a BLNK ortholog gene from the orange-spotted grouper (Epinephelus coioides). Homology analysis showed that the grouper BLNK (EcBLNK) had a 34%-77% amino acid identity in comparison to other vertebrates and shared the highest amino acid identity with BLNK from the Asian seabass Lates calcarifer. EcBLNK comprises an N-terminal SAM domain and a C-terminal B-cell linker SH2 domain...
July 25, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30041050/molecular-cloning-and-characterization-of-aos1-and-uba2-from-the-orange-spotted-grouper-epinephelus-coioides
#3
Jingguang Wei, Chen Li, Xin Zhang, Sheng Zhou, Shaoqing Zang, Shina Wei, Qiwei Qin
Small ubiquitin-related modifiers (SUMOs) are post-translationally conjugated to other proteins and are essential regulators of a wide range of cellular processes. Covalent attachment of SUMO requires an enzymatic cascade consisting of a single E1-activating enzyme (Aos1 and Uba2 heterodimer), a single E2-conjugating enzyme (Ubc9), and one of several E3 ligases that facilitate transfer of SUMO from Ubc9 to the substrate. In the present study, the Aos1 and Uba2 homologues (EcAos1 and EcUba2) from the orange-spotted grouper (Epinephelus coioides) were cloned and their possible roles in fish immunity were analyzed...
July 21, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30030116/cottonseed-protein-concentrate-cpc-suppresses-immune-function-in-different-intestinal-segments-of-hybrid-grouper-%C3%A2-epinephelus-fuscoguttatus%C3%A3-%C3%A2-epinephelus-lanceolatu-via-tlr-2-myd88-signaling-pathways
#4
Bin Yin, Hongyu Liu, Beiping Tan, Xiaohui Dong, Shuyan Chi, Qihui Yang, Shuang Zhang, Liqiao Chen
Cottonseed protein concentrate (CPC) has similar amino acid composition compared with fish meal, and has the characteristics of low gossypol and low toxicity. The present study was conducted to investigate the growth performance, antioxidant capacity and different intestinal segments immune responses of hybrid grouper to replacement dietary fish meal ofCPC. Six iso-nitrogenous (50% crude protein) and iso-lipidic (10% crude lipid) diets were formulated: a reference diet (FM) containing 60% fishmeal and five experimental diets (12%, 24%, 36%, 48 and 60%) in which fishmeal protein was substituted at different levels by CPC to feed fish (initial body weight: 11 ± 0...
July 18, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30016710/interferon-regulatory-factor-3-from-sea-perch-lateolabrax-japonicus-exerts-antiviral-function-against-nervous-necrosis-virus-infection
#5
Wanwan Zhang, Zelin Li, Peng Jia, Wei Liu, Meisheng Yi, Kuntong Jia
Interferon (IFN) regulatory factor 3 (IRF3) is a major regulator contributing to the host away from viral infection. Here, an IRF3 gene from sea perch (LjIRF3) was identified and its role in regulating early apoptosis signaling and IFN response was investigated during red spotted grouper nervous necrosis virus (RGNNV) infection. The cDNA of LjIRF3 encoded a putative 465 amino acids protein, containing a DNA binding domain, an IRF association domain and a serine-rich domain. Phylogenetic analysis suggested that LjIRF3 shared the closest genetic relationship with Epinephelus coioides IRF3...
July 26, 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/30010020/molecular-identification-and-functional-characterization-of-irak-3-from-a-teleost-fish-the-orange-spotted-grouper-epinephelus-coioides
#6
Yan-Wei Li, Rui Han, Jiu-Le Wang, Man Yang, Xue-Ming Dan, An-Xing Li
Interleukin-1 receptor-associated kinase-3 (IRAK-3) is a unique IRAK family member, which negatively regulates the TLR-mediated immune response in mammals. However, the function of IRAK-3 remains to be elucidated in fish. In the present study, an IRAK-3 cDNA sequence (EcIRAK-3) with an ORF of 1776 bp encoding 591 amino acids was identified in the orange-spotted grouper (Epinephelus coioides). Sequence analysis indicated that EcIRAK-3 shared the conserved structure characteristics and functional sites of vertebrate IRAK-3, and has a high sequence identity and phylogenetic relationship with that of other fish species...
July 26, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30006040/integration-of-rnai-and-rna-seq-uncovers-the-immune-responses-of-epinephelus-coioides-to-l321_rs19110-gene-of-pseudomonas-plecoglossicida
#7
Beibei Zhang, Gang Luo, Lingmin Zhao, Lixing Huang, Yingxue Qin, Yongquan Su, Qingpi Yan
Pseudomonas plecoglossicida is a threatening and important pathogen in aquaculture and causes heavy losses. Expression of L321_RS19110 was found significant up-regulated at 18 °C than at 28 °C, which was confirmed by quantitative real-time PCR. RNAi significantly reduced the content of L321_RS19110 mRNA of P. plecoglossicida, and exhibited the best efficiency of gene silencing with a reduction of 84.9%. Compared with the wild type strain, the infection of L321_RS19110-RNAi-1 strain resulted in the onset time delay, and 30% reduction in mortality of Epinephelus coioides, as well as alleviates in the symptoms of E...
July 10, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29981882/nile-tilapia-fry-fed-on-antimicrobial-peptide-epinecidin-1-expressing-artemia-cyst-exhibit-enhanced-immunity-against-acute-bacterial-infection
#8
Chen-Hung Ting, Yi-Chun Chen, Jyh-Yih Chen
Artemia are often used as a live feed for fry in aquaculture. We have previously demonstrated that supplementing adult zebrafish feed with Artemia, which express an Epinephelus coioides-derived antimicrobial peptide, Epinecidin-1 (Epi-1), protects against bacterial infection. Thus, Artemia may serve as a bioreactor for producing biofunctional molecules. However, the application of Epi-1 transgenic Artemia in larval aquaculture of commercial fish species has not been investigated. Here we used a Tol2-transposon system to generate stable Epi-1 expressing Artemia...
July 5, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29926922/ecological-and-histopathological-aspects-of-didymodiclinus-sp-trematoda-didymozoidae-parasite-of-the-dusky-grouper-epinephelus-marginatus-osteichthyes-serranidae-from-the-western-mediterranean-sea
#9
Marta Polinas, Salvatore Mele, Francesc Padrós, Paolo Merella, Elisabetta Antuofermo, Adam Gouraguine, Olga Reñones
The dusky grouper Epinephelus marginatus (Lowe) is an ecologically and commercially important fish species of the Atlantic and Mediterranean coastal rocky habitats. Despite records of didymozoid infections in several grouper species, the identification and pathogenesis of these parasites in E. marginatus are lacking. The aim of this study is to characterize the didymozoids of E. marginatus, particularly their mechanisms of infection and histopathological features. Dusky groupers (n = 205) were caught off Majorca Island (western Mediterranean Sea) and examined for parasites...
June 21, 2018: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29913207/orange-spotted-grouper-epinephelus-coioides-that-have-encountered-low-salinity-stress-have-decreased-cellular-and-humoral-immune-reactions-and-increased-susceptibility-to-vibrio-alginolyticus
#10
Yu-Yuan Chen, Ann-Chang Cheng, Shao-An Cheng, Jiann-Chu Chen
Orange-spotted grouper Epinephelus coioides reared at 34‰ and 27 °C were abruptly transferred to 6‰, 20‰ and 34‰ (control) and examined for innate cellular and humoral parameters after 3-96 h. Total leucocyte count (TLC), respiratory burst (RB), phagocytic activity (PA), alternative complement pathway (ACP) and lysozyme activity were significantly decreased 3-6 h, 3-6 h, 3-96 h, 3-96 h and 3-96 h, respectively after transferal into 6‰ salinity. TLC, RB and PA significantly increased after 3-48 h, 3-96 h and 3-24 h, respectively, with recovery of TLC and PA after 96 h and 48-96 h, whereas ACP and lysozyme activity significantly decreased 3-96 h after being transferred to 20‰...
September 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29882295/enhanced-immune-responses-and-effectiveness-of-refined-outer-membrane-protein-vaccines-against-vibrio-harveyi-in-orange-spotted-grouper-epinephelus-coioides
#11
Hai Trong Nguyen, Thuy Thi Thu Nguyen, Yan-Chun Chen, Hung Vu-Khac, Pei-Chi Wang, Shih-Chu Chen
Vibriosis is a severe infection occurring in many commercially important marine fish species. In this study, vaccines containing Vibrio harveyi recombinant outer membrane protein K (rOmpK), outer membrane protein U (rOmpU) and rOmpK-OmpU fusion protein in addition to the metabolizable MontanideTM ISA 763 A VG adjuvant were developed and evaluated in the orange-spotted grouper. The results indicate that recombinant V. harveyi protein-based vaccines resulted in a remarkably higher expression of IL-1β and IL-8 at 24 hr, and greater antibody production, as early as 2 weeks postimmunization...
June 8, 2018: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29798731/dietary-arginine-affects-growth-gut-morphology-oxidation-resistance-and-immunity-of-hybrid-grouper-epinephelus-fuscoguttatus%C3%A2-%C3%A3-epinephelus-lanceolatus%C3%A2-juveniles
#12
Mingjuan Wu, Xiaoyi Wu, Senda Lu, Yujie Gao, Wei Yao, Xiaojun Li, Yu Dong, Zibo Jin
An 8-week growth trial was conducted to evaluate the effects of dietary arginine (Arg) levels on growth, gut morphology, oxidation resistance and immunity of hybrid grouper (Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂) juveniles. Seven isoenergetic (1465 kJ (350 kcal)/100-g DM), isoproteic (53·5 % of DM) and isolipidic (7 % of DM) experimental diets were formulated to contain graded Arg levels ranging from 1·9 to 4·7 % (dry weight) at approximately 0·5 % increments. Each diet was randomly assigned to triplicate groups of 16 juvenile fish (average initial body weight: 11·7 (sd 0·1) g) and was administered twice daily (08...
August 2018: British Journal of Nutrition
https://www.readbyqxmd.com/read/29567142/molecular-characterization-and-function-analysis-of-grouper-epinephelus-coioides-bruton-s-tyrosine-kinase-btk
#13
Ze-Quan Mo, Qing Han, Yu-Long Zeng, Jiu-Le Wang, Xue-Zhu Li, Yan-Wei Li, Hong-Yan Sun, An-Xing Li, Xiao-Chun Luo, Xue-Ming Dan
Bruton's tyrosine kinase (BTK) is a Tec-family tyrosine kinase and plays a crucial role in B cell antigen receptor (BCR) signal pathway. Mutations in humans and mice BTK gene results in X-linked agammaglobulinemia (XLA) and X-linked immunodeficiency (XLD), respectively. To study the function of BTK in teleost, we cloned a BTK gene from orange-spotted grouper. Homology analysis showed that the grouper BTK (EcBTK) had a high amino acid identity with other vertebrates (63%-92%) and shared the highest amino acid identity with ballan wrasse Labrus bergylta BTK...
June 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29535680/two-distinct-interferon-%C3%AE-in-the-orange-spotted-grouper-epinephelus-coioides-molecular-cloning-functional-characterization-and-regulation-in-toll-like-receptor-pathway-by-induction-of-mir-146a
#14
Wan Peng, Yan Sun, Gao-Fei Li, Liang-Ge He, Ruo-Zhu Li, Yao-Si Liang, Xu Ding, Xue Yu, Yong Zhang, Hao-Ran Lin, Dan-Qi Lu
Interferon gamma (IFNγ) is a Th1 cytokine that is critical for innate and adaptive immunity. Toll-like receptors (TLRs) signaling pathways are critical in early host defense against invading pathogens. miR-146a has been reported to participate in the regulation of host immunity. The known mechanisms of integrations between the IFNγ and TLR signaling pathways are incompletely understood, especially in teleosts. In this study, orange-spotted grouper ( Epinephelus coioides ) IFNγ1 and IFNγ2, their biological activities, especially their involvements in TLR pathway, were explored...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29510258/recombinant-epinephelus-lanceolatus-serum-amyloid-a-as-a-feed-additive-effects-on-immune-gene-expression-and-resistance-to-vibrio-alginolyticus-infection-in-epinephelus-lanceolatus
#15
Bor-Chyuan Su, Wen-Chun Lin, Jyh-Yih Chen
Recombinant Epinephelus lanceolatus serum amyloid A (rElSAA) exhibits strong immunostimulant activity and enhances phagocytic clearance of bacteria by macrophages. However, the effects of dietary rElSAA supplementation on growth performance, immunomodulation and disease resistance in giant grouper have not been previously evaluated. To test whether oral administration of rElSAA affects growth, fish were fed with 0, 0.88, 4.4 or 22 mg/kg rElSAA-containing diet for 28 days. No statistically significant differences in body weight were observed between groups...
May 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29475509/oral-immunization-with-cell-free-self-assembly-virus-like-particles-against-orange-spotted-grouper-nervous-necrosis-virus-in-grouper-larvae-epinephelus-coioides
#16
Ming-Hui Chien, Shaw-Yun Wu, Cheng-Hui Lin
Nervous necrosis virus (NNV) infection causes viral nervous necrosis, inflicting serious economic losses in marine fish cultivation. Vaccination is the most effective choice for controlling and preventing viral infection. Virus-like particles (VLPs) are considered a novel vaccine platform because they are not infectious and they induce neutralizing antibodies efficiently. In the present study, we investigated the effect of the recombinant orange-spotted grouper NNV (OSGNNV) capsid proteins produced in Escherichia coli and cell-free self-assembled into VLPs on protective immune responses in orange-spotted grouper following immersion, intramuscular injection and oral immunization...
March 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29454897/involvement-of-eif2%C3%AE-of-epinephelus-coioides-in-the-fish-immune-response-to-virus-infection
#17
Shaoqing Zang, Xin Zhang, Jingcheng Zhang, Chen Li, Jingguang Wei, Qiwei Qin
The eukaryotic initiation factor 2 alpha subunit (eIF2α) is a key translation regulator that plays an important role in different cellular pressures and stimuli, including virus infection. In the present study, an eIF2α homolog (EceIF2α) from the orange-spotted grouper (Epinephelus coioides) was cloned and its roles during fish viral infection were characterized. EceIF2α encodes a putative protein of 315 amino acid residues, and shares a high degree of similarity with eIF2αs from other species. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that EceIF2α was distributed in all examined tissues...
April 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29427720/construction-of-a-vibrio-alginolyticus-hoppmaj-hop-mutant-and-evaluation-of-its-potential-as-a-live-attenuated-vaccine-in-orange-spotted-grouper-epinephelus-coioides
#18
Huanying Pang, Mingsheng Qiu, Jingmin Zhao, Rowena Hoare, Sean J Monaghan, Dawei Song, Yunsheng Chang, Jichang Jian
Vibrio alginolyticus, a bacterial pathogen in fish and humans, expresses a type III secretion system (T3SS) that is critical for pathogen virulence and disease development. However, little is known about the associated effectors (T3SEs) and their physiological role. In this study, the T3SE gene hopPmaJ (hop) was cloned from V. alginolyticus wild-type strain HY9901 and the mutant strain HY9901Δhop was constructed by the in-frame deletion method. The results showed that the deduced amino acid sequence of V. alginolyticus HopPmaJ shared 78-98% homology with other Vibrio spp...
May 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29355760/molecular-cloning-and-characterization-of-fadd-from-the-orange-spotted-grouper-epinephelus-coioides
#19
Xin Zhang, Shaoqing Zang, Chen Li, Jingguang Wei, Qiwei Qin
Fas-associated protein with death domain (FADD) is the key adaptor protein that transmits apoptotic signals mediated by the main death receptors. Besides being an essential instrument in cell death, FADD is also implicated in proliferation, cell cycle progression, tumor development, inflammation, innate immunity, and autophagy. In the present study, a FADD homologue (EcFADD) from the orange-spotted grouper (Epinephelus coioides) was cloned and its possible role in fish immunity was analyzed. The full length cDNA of EcFADD contains 808 base pairs (bp), including a 573 bp open reading frame that encodes a 190 amino acid protein with a predicted molecular mass of 21...
March 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29330509/transcriptome-analysis-reveals-carbohydrate-mediated-liver-immune-responses-in-epinephelus-akaara
#20
Yunxia Yang, Tao Han, Jia Xiao, Xinyu Li, Jiteng Wang
As the cheapest energy source, carbohydrates are used in fish feeds to improve physical quality and reduce catabolism of proteins and lipids. The liver is the primary organ for metabolism and is also an important site of immune regulation. Here, we investigated the effect of different dietary carbohydrate levels on growth and health by evaluating the liver transcriptome of Epinephelus akaara. In this study, E. akaara juveniles were fed diets containing few (0% corn starch), moderate (18% corn starch), and high (30% corn starch) levels of dietary carbohydrate...
January 12, 2018: Scientific Reports
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