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Nurliyana Mohamad, Fauzul Aidil Mohd Roseli, Mohammad Noor Amal Azmai, Mohd Zamri Saad, Ina Salwany Md Yasin, Nor Amalina Zulkiply, Nurrul Shaqinah Nasruddin
In September 2016, a marine fish farm operator in Selangor, Malaysia reported a disease outbreak affecting juvenile Hybrid Groupers (Camouflage Grouper Epinephelus polyphekadion x Tiger Grouper E. fuscoguttatus). The average daily mortality was 120 fish, resulting in a cumulative mortality rate of 29% within 10 days. The affected Hybrid Groupers displayed lethargy, excessive mucus production, rotten fins, congestion of livers and kidneys, and enlargement of spleens. Microscopically, there were generalize congestion of the brains and internal organs...
December 7, 2018: Journal of Aquatic Animal Health
Ka Yan Ma, Lynne van Herwerden, Stephen J Newman, Michael L Berumen, John Howard Choat, Ka Hou Chu, Yvonne Sadovy de Mitcheson
BACKGROUND: Understanding the factors shaping population genetic structure is important for evolutionary considerations as well as for management and conservation. While studies have revealed the importance of palaeogeographic changes in shaping phylogeographic patterns in multiple marine fauna, the role of reproductive behaviour is rarely considered in reef fishes. We investigated the population genetics of three commercially important aggregating grouper species in the Indo-West Pacific, namely the camouflage grouper Epinephelus polyphekadion, the squaretail coral grouper Plectropomus areolatus, and the common coral trout P...
December 4, 2018: BMC Evolutionary Biology
Witchuda Prasatkaew, Phochit Nanthanawat, Nanthika Khongchareonporn, Sutin Kingtong
A monoclonal antibody specific to sea bass (Lates calcarifer) vitellogenin (VTG) was developed, for use as a tool for monitoring endocrine disrupting chemicals (EDCs). VTG was induced in sea bass by intramuscular injection of 17β-estradiol (E2 : 2 mg/kg) every three days. Blood was collected three days after the last injection. Plasma VTG was then purified by chromatography in hydroxyapatite and a sephacryl-S300 column. Characterizations of purified VTG were done by phospholipoglycoprotein staining on a native-PAGE with confirmation by mass spectrometry (LC-MS/MS)...
January 2019: Journal of Environmental Sciences (China)
Sheng Zhou, Deli Song, Xiaofeng Zhou, Xinliang Mao, Xuefeng Zhou, Sunli Wang, Jingguang Wei, Youhua Huang, Wenxiong Wang, Su-Mei Xiao, Qiwei Qin
Probiotics are widely used for the improvement of animals' growth and health. However, few marine aquatic probiotics are applied and licensed in China. In this study, a Bacillus spp. strain was isolated from the Hulong grouper gastrointestinal tract, which was identified as a new strain of Bacillus subtilis and was named as 7k. B. subtilis 7k showed desirable capability of sporulation and resistance to heat, simulated gastric juice and simulated duodenum juice, indicating its potential as probiotics. Seven antimicrobial chemicals were found in the secretion of the B...
October 26, 2018: Fish & Shellfish Immunology
Quanxin Gao, Yanfeng Yue, Minghua Min, Shiming Peng, Zhaohong Shi, Jinbo Wang, Tao Zhang
The Kelp grouper Epinephelus moara is one of the most widely consumed and economically important marine fish in China. The species can tolerate a wide range of salinity, but genomic resources are not available, and the molecular mechanisms underlying adaptation to salinity at the transcriptomic level remain largely unclear. In this study, the transcriptomic responses of the liver of E. moara under low salinity were investigated using the Illumina digital gene expression system. After de novo assembly, 499,356 transcripts were generated and contributed 445,068 unigenes...
2018: Animal Cells and Systems
Ling Xiao, Dengdong Wang, Yin Guo, Zhujing Tang, Qiongyu Liu, Shuisheng Li, Yong Zhang, Haoran Lin
In our previous studies, diploid and triploid hybrids have been detected from the hybridization of Epinephelus coioides♀ × E. lanceolatus♂. The triploid groupers have been found to be delayed in gonadal development, but the mechanism remains poorly understood. In this study, we examined the gonadal development, assayed the serum steroid hormone levels, and compared the BP (brain and pituitary) and G (gonad) transcriptomes of 18-month-old diploid and triploid hybrids. The results showed that levels of serum estradiol-17β and testosterone were significantly higher in triploid groupers...
November 16, 2018: Genomics
Zhiming Zhu, Chuanfu Dong, Shaoping Weng, Jianguo He
Vibrio harveyi is a serious pathogen of scale drop and muscle necrosis disease in marine commercial fishes. Adhesion to and colonization of the host cells surfaces is the first and crucial step for pathogenic bacterial infection, which is usually mediated by outer membrane proteins (Omps). The objectives of this study were to identify the major adhesin in Omps that plays the essential role in adhesion of V. harveyi to the host cells, and to assess the potential of this adhesin as a vaccine candidate for V. harveyi infection...
November 16, 2018: Fish & Shellfish Immunology
Ze-Quan Mo, Rui Han, Jiu-Le Wang, Lu-Yun Ni, Yu-Ling Su, Xue-Li Lai, Zhi-Chang He, Hong-Ping Chen, Yan-Wei Li, Hong-Yan Sun, Xiao-Chun Luo, Xue-Ming Dan
MEK dual-specificity protein kinases are a group of mitogen-activated protein kinase kinases, which act as an integration point by transferring extracellular signals to the nucleus. To investigate the function of MEK in teleost fish, we cloned MEK1 and MEK2 cDNA sequences from the orange-spotted grouper (Epinephelus coioides). EcMEK1 and EcMEK2 shared 80% amino acid identity with each other. EcMEK1 had 89-99% amino acid identity with teleosts or mammals, whereas EcMEK2 shared 85-97% amino acid identity. The exon structures of the grouper MEK1/2 genes were conserved with zebrafish and human MEK1/2...
November 9, 2018: Fish & Shellfish Immunology
Minwei Huang, Qing Wang, Jiaxing Chen, Huimin Chen, Ling Xiao, Mi Zhao, Haifa Zhang, Shuisheng Li, Yun Liu, Yong Zhang, Haoran Lin
Estrogen plays a pivotal role in the sex differentiation of teleosts, whereas the precise function of androgens is more controversial. In this study, orange-spotted grouper (Epinephelus coioides) fry were treated with letrozole (an aromatase inhibitor, AI), 17α-methyltestosterone (MT), or MT and 17β-estradiol (E2) simultaneously, during the period of gonadal formation and sex differentiation. MT-feeding at 50 days after hatching resulted in gonadal dysgenesis, which could be rescued by E2 supplementation...
November 10, 2018: Biology of Reproduction
Fenny Crista A Panjaitan, Honey Lyn R Gomez, Yu-Wei Chang
Major proteins contained in dried giant grouper roe (GR) such as vitellogenin (from Epinephelus coioides ; NCBI accession number: AAW29031.1), apolipoprotein A-1 precursor (from Epinephelus coioides ; NCBI accession number: ACI01807.1) and apolipoprotein E (from Epinephelus bruneus ; NCBI accession number: AEB31283.1) were characterized through compiled proteomics techniques (SDS-PAGE, in-gel digestion, mass spectrometry and on-line Mascot database analysis). These proteins were subjected to in silico analysis using BLAST and BIOPEP-UWM database...
November 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Peter Palma, Josephine Nocillado, Joshua Superio, Evelyn Grace de Jesus Ayson, Felix Ayson, Ido Bar, Abigail Elizur
The role of follicle stimulating hormone (FSH) in the gonadal development of protogynous hermaphroditic grouper (E. fuscoguttatus) was investigated. Recombinant giant grouper (E. lanceolatus) FSH (rggFSH) was produced in yeast. Its receptor binding capacity and steroidogenic potency were confirmed in vitro. Weekly injections of rggFSH to juvenile tiger grouper for 8 weeks (100 μg/kg body weight, BW) resulted in significantly larger and more advanced oocytes (cortical alveolar stage vs. primary growth stage in control)...
October 27, 2018: Biology of Reproduction
Shina Wei, Shaowen Wang, Min Yang, Youhua Huang, Jingguang Wei, Xiaohong Huang, Qiwei Qin
The lysosomal cysteine protease cathepsin C plays a pivotal role in regulation of inflammatory and immune responses. However, the function of fish cathepsin C in virus replication remains largely unknown. In this study, cathepsin C gene (Ec-CC) was cloned and characterized from orange-spotted grouper, Epinephelus coioides. The full-length Ec-CC cDNA was composed of 2077 bp. It contained an open reading frame (ORF) of 1374 bp and encoded a 458-amino acid protein which shared 89% identity to cathepsin C from bicolor damselfish (Stegastes partitus)...
October 9, 2018: Fish & Shellfish Immunology
Mohamed Afifi, Ali Alkaladi, Osama A Abu Zinada, Michel Couderchet
The mRNA expression profile of some antioxidant genes in skin, gills, livers, and muscles of Siganus canaliculatus and Epinephelus morio was used as an indicator of petroleum hydrocarbons pollution in six areas at Jeddah and Yanbu coasts in KSA. Total petroleum hydrocarbons (TPHs) were determined in both sea water and sediments collected from the studied areas. The mRNA expression levels of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were determined...
November 2017: Saudi Journal of Biological Sciences
Scott C Cutmore, Matthew J Nolan, Thomas H Cribb
Eight species of the trematode family Bucephalidae Poche, 1907 are reported from teleost fishes in Moreton Bay, Queensland, Australia. Heterobucephalopsis yongi n. sp. is described from Gymnothorax eurostus (Muraenidae); the new form is distinguished from its congeners in the possession of a tiny cirrus-sac relative to body length, the length of the caecum, the position of the mouth and pharynx, and the position of the testes and ovary. Two known species of Dollfustrema Eckmann, 1934, D. durum Nolan, Curran, Miller, Cutmore, Cantacessi & Cribb, 2015 and D...
November 2018: Systematic Parasitology
Wei-Jia Lu, Li Zhou, Fan-Xiang Gao, Zhi-Hui Sun, Zhi Li, Xiao-Chun Liu, Shui-Sheng Li, Yang Wang, Jian-Fang Gui
Chemokine receptor Cxcr4 evolved two paralogs in the teleost lineage. However, cxcr4a and cxcr4b have been characterized only in a few species. In this study, we identified two cxcr4 paralogs from the orange-spotted grouper, Epinephelus coioides . The phylogenetic relationship and gene structure and synteny suggest that the duplicated cxcr4a/b should result from the teleost-specific genome duplication (Ts3R). The teleost cxcr4 gene clusters in two paralogous chromosomes exhibit a complementary gene loss/retention pattern...
September 27, 2018: International Journal of Molecular Sciences
Sheng-Wei Luo, Huan Kang, Ren-Chong Xie, Wei Wei, Qing-Jian Liang, Yuan Liu, Wei-Na Wang
Complement 1 inhibitor (C1INH) serving as a multifunctional factor can participate in the regulation of complement cascades and attenuate the activation of various proteases. In this study, we obtained EcC1INH cDNA and the tissue-specific analysis indicate that the highest expression level of EcC1INH mRNA was detected in liver. Moreover, Vibrio alginolyticus challenge can significantly increase EcC1INH mRNA expression in liver and kidney. N-terminal domain of EcC1INH could decrease LPS binding activity to cell surface, while loss of positively charged residues (PCRs) Arg21, His22, Lys50, Arg61 in N-terminal domain of EcC1INH can significantly reduce its interaction with LPS...
September 24, 2018: Fish & Shellfish Immunology
D Alvarez-Berastegui, J Coll, L Rueda, B Stobart, G Morey, O Navarro, A Aparicio-González, A M Grau, O Reñones
Describing the spatial patterns of benthic coastal habitats and investigating how those patterns affect the ecology of inhabiting species is a main objective of seascape ecology. Within this emerging discipline spatial scale is a principal topic. Different spatial scales inform on different characteristics of the habitat and therefore the relation between species and their habitats would be better defined when observed at multiple levels of spatial scale. Here we apply a multiscale seascape approach to investigate the habitat preferences of juvenile and adult individuals of dusky grouper (Epinephelus marginatus) in a Mediterranean marine protected area...
September 5, 2018: Marine Environmental Research
Ya-Zhou Hu, Xia Li, Rui Han, Biao Jiang, Yan-Wei Li, Xue-Ming Dan, An-Xing Li
Transforming growth factor-β activated kinase 1 (TAK1) is a crucial signal transducer in multiple signaling pathways. TAK1 binds TAB1, TAB2, and TAB3, which act as activators and adaptors that specifically regulate the activation of TAK1. To date, the role of TABs is largely unknown in fish. In the present study, a TAB1 cDNA sequence was identified in grouper (Epinephelus coioides), and designated EcTAB1. The full-length open reading frame of EcTAB1 is 1, 521 bp; it encodes 506 amino acids that contains an N-terminal PP2C domain...
January 2019: Developmental and Comparative Immunology
Qi Zheng, Huasong Ji, Shina Wei, Jufen Tang, Yishan Lu, Jia Cai, Jichang Jian, Qiwei Qin
Bcl-2 family proteins play essential roles in modulating immune response and controlling cells' fate. Bcl-xL is one of anti-apoptotic protein in this family. In this study, a new Bcl-xL homolog (EcBcl-xL) was identified and characterized from orange-spotted grouper, Epinephelus coioides. EcBcl-xL encoded a 221 amino acid peptides that shared 86% identity to Larimichthys crocea Bcl-xL protein, contained four conserved BH domains and one transmembrane region. The predicted three-dimensional structure of EcBcl-xL was similar with Homo sapiens Bcl-xL...
December 2018: Fish & Shellfish Immunology
Qing Wang, Minwei Huang, Cheng Peng, Xiang Wang, Ling Xiao, Dengdong Wang, Jiaxing Chen, Huihong Zhao, Haifa Zhang, Shuisheng Li, Huirong Yang, Yun Liu, Haoran Lin, Yong Zhang
In this study, we systematically investigated the process of sex reversal induced by 17-methyltestosterone (MT) feeding and MT-feeding withdrawal at the ovary differentiation stage in orange-spotted groupers, Epinephelus coioides . Gonadal histology showed that MT feeding induced a precocious sex reversal from immature ovaries to testes, bypassing the formation of an ovarian cavity, and MT-feeding withdrawal led to an ovarian fate. In both the MT feeding and MT-feeding withdrawal phases, cytochrome P450 family 11 subfamily B ( cyp11b ) gene expression and serum 11-KT levels were not significantly changed, suggesting that the MT-treated fish did not generate endogenous steroids, even though active spermatogenesis occurred...
September 19, 2018: International Journal of Molecular Sciences
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