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https://www.readbyqxmd.com/read/29454897/involvement-of-eif2%C3%AE-of-epinephelus-coioides-in-the-fish-immune-response-to-virus-infection
#1
Shaoqing Zang, Xin Zhang, Jingcheng Zhang, Chen Li, Jingguang Wei, Qiwei Qin
The eukaryotic initiation factor 2 alpha subunit (eIF2α) is a key translation regulator that plays an important role in different cellular pressures and stimuli, including virus infection. In the present study, an eIF2α homolog (EceIF2α) from the orange-spotted grouper (Epinephelus coioides) was cloned and its roles during fish viral infection were characterized. EceIF2α encodes a putative protein of 315 amino acid residues, and shares a high degree of similarity with eIF2αs from other species. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that EceIF2α was distributed in all examined tissues...
April 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29355760/molecular-cloning-and-characterization-of-fadd-from-the-orange-spotted-grouper-epinephelus-coioides
#2
Xin Zhang, Shaoqing Zang, Chen Li, Jingguang Wei, Qiwei Qin
Fas-associated protein with death domain (FADD) is the key adaptor protein that transmits apoptotic signals mediated by the main death receptors. Besides being an essential instrument in cell death, FADD is also implicated in proliferation, cell cycle progression, tumor development, inflammation, innate immunity, and autophagy. In the present study, a FADD homologue (EcFADD) from the orange-spotted grouper (Epinephelus coioides) was cloned and its possible role in fish immunity was analyzed. The full length cDNA of EcFADD contains 808 base pairs (bp), including a 573 bp open reading frame that encodes a 190 amino acid protein with a predicted molecular mass of 21...
March 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29097235/unc93b1-is-essential-for-cytokine-activation-of-five-pamps-in-the-orange-spotted-grouper-epinephelus-coioides
#3
Yaosi Liang, Yu Wang, Liangge He, Jianan He, Wan Peng, Libin Zhou, Yong Zhang, Haifa Zhang, Herong Shi, Haoran Lin, Danqi Lu
Toll-like receptors (TLRs) are important innate immune receptors that recognize multiple pathogen-associated molecular patterns (PAMPs) and activate the immune responses to resist the invasion of pathogens. Many TLRs need assistance from trafficking chaperones to transport to the specific cell compartments and then are processed before they are activated. In this study, we identified an important trafficking chaperone, Unc-93 homolog B1 (unc93b1), from the Epinephelus coioides (orange-spotted grouper). The deduced protein sequence of Eco...
April 2018: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/28964860/fish-ddx3x-exerts-antiviral-function-against-grouper-nervous-necrosis-virus-infection
#4
Jiaxin Liu, Xiaohong Huang, Yepin Yu, Jingcheng Zhang, Songwei Ni, Yin Hu, Youhua Huang, Qiwei Qin
Human DEAD box ATP-dependent RNA helicase DDX3X has been demonstrated to exert crucial functions in carcinogenesis and antiviral immune response. However, to our knowledge, few information focused on the functions of fish DDX3X. In this study, we cloned and characterized a DDX3X homolog from orange spotted grouper (Epinephelus coioides) (EcDDX3X). EcDDX3X encoded a 733-amino acid protein which shared 97% and 76% identity to spiny damselfish (Acanthochromis polyacanthus) and human (Homo sapiens), respectively...
December 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28964858/expression-and-functional-characterization-of-trif-in-orange-spotted-grouper-epinephelus-coioides
#5
Jingguang Wei, Xin Zhang, Shaoqing Zang, Qiwei Qin
Antiviral immune responses are triggered by the innate immune recognition of viral infection. Toll/interleukin-1 receptor (TIR) domain containing adapter inducing interferon-β (TRIF) is an adapter in responding to activation of Toll-like receptors, which provides early clearance of viral pathogens. Our study focuses on the functional characterization of grouper TRIF (EcTRIF) based on the comparison of its sequence and functional evolution from grouper fish to mammals. The results show that the open reading frame of EcTRIF encoded a protein of 580 amino acids...
December 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27773730/molecular-cloning-of-orange-spotted-grouper-epinephelus-coioides-heat-shock-transcription-factor-1-isoforms-and-characterization-of-their-expressions-in-response-to-nodavirus
#6
Ting-Yu Wang, Young-Mao Chen, Tzong-Yueh Chen
Heat shock transcription factor 1 (HSF1) regulates heat shock proteins (HSPs), which assist in protein folding and inhibit protein denaturation following stress. HSF1 was firstly cloned from orange-spotted grouper and exists as two isoforms, one with (osgHSF1a) and one without (osgHSF1b) exon 11. Heat exposure increased the expression of osgHSF1b while cold exposure increased that of osgHSF1a. Both isoforms were mainly expressed in the brains, eyes, and fins. Expression of osgHSF1b was higher than osgHSF1a during development...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27751918/molecular-and-functional-characterization-of-toll-like-receptor-21-in-large-yellow-croaker-larimichthys-crocea
#7
Min Sun, Yinnan Mu, Yang Ding, Jingqun Ao, Xinhua Chen
Toll-like receptor 21 (TLR21) is a non-mammalian TLR that functions similar to mammalian TLR9 in recognizing CpG DNA. In the present study, we identified a TLR21 homologue, LycTLR21, from large yellow croaker (Larimichthys crocea). The complete coding sequence of LycTLR21 is 2946 nucleotides long, encoding a protein of 981 amino acids. The deduced LycTLR21 protein has typical TLR domain architecture, including a signal peptide, 13 leucine-rich repeats (LRRs) in the extracellular region, a transmembrane region, and a cytoplasmic Toll-Interleukin-1 receptor (TIR) domain...
December 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27666189/a-member-of-the-immunoglobulin-superfamily-orange-spotted-grouper-novel-immune-gene-ecvig-is-induced-by-immune-stimulants-and-type-i-interferon
#8
Ying-Chun Yeh, Ting-Yu Wang, Hsin-Yiu Chou, Han-You Lin, Tzong-Yueh Chen, Takashi Aoki, Han-Ching Wang
A novel grouper immune gene, EcVig was identified in orange-spotted grouper (Epinephelus coioides). We recently determined that EcVig expression can be induced by infection with nervous necrosis virus (NNV, an RNA virus), whereas NNV replication may be suppressed when EcVig was overexpressed. Although EcVig appeared to be involved in grouper antiviral activity, its immune effects have not been well characterized. In the present study, two PAMPs (pathogen-associated molecular patterns; lipopolysaccharides [LPS] and synthetic double-stranded RNA polyriboinosinic-polyribocytidylic acid [poly(I:C)]), as well as fish DNA virus (red sea bream iridovirus, RSIV; grouper iridovirus, GIV), were used to study EcVig responses in orange-spotted grouper...
November 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27601297/mkk7-confers-different-activities-to-viral-infection-of-singapore-grouper-iridovirus-sgiv-and-nervous-necrosis-virus-nnv-in-grouper
#9
Minglan Guo, Jingguang Wei, Yongcan Zhou, Qiwei Qin
Mitogen-activated protein kinase 7 (MKK7) is one of the major stress-activated protein kinase (SAPK)-activating kinases in response to environmental or physiological stimuli. Here a MKK7 named as Ec-MKK7 was identified from orange-spotted grouper, Epinephelus coioides. The full-length cDNA of Ec-MKK7 was 1853 bp, with an open reading frame (ORF) of 1272 bp encoding a putative protein of 423 amino acids. A characteristic S-K-A-K-T motif was contained in the domain of dual-specificity protein kinase, mitogen-activated protein kinase kinase 7 (PKc_MKK7)...
October 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27235367/grouper-trim13-exerts-negative-regulation-of-antiviral-immune-response-against-nodavirus
#10
Youhua Huang, Min Yang, Yepin Yu, Ying Yang, Linli Zhou, Xiaohong Huang, Qiwei Qin
The tripartite motif (TRIM)-containing proteins have attracted particular attention to their multiple functions in different biological processes. TRIM13, a member of the TRIM family, is a RING domain-containing E3 ubiquitin ligase which plays critical roles in diverse cellular processes including cell death, cancer and antiviral immunity. In this study, a TRIM13 homolog from orange spotted grouper, Epinephelus coioides (EcTRIM13) was cloned and characterized. The full-length of EcTRIM13 cDNA encoded a polypeptide of 399 amino acids which shared 81% identity with TRIM13 homolog from large yellow croaker (Larimichthys crocea)...
August 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27150052/fish-trim8-exerts-antiviral-roles-through-regulation-of-the-proinflammatory-factors-and-interferon-signaling
#11
Youhua Huang, Yepin Yu, Ying Yang, Min Yang, Linli Zhou, Xiaohong Huang, Qiwei Qin
The tripartite motif (TRIM)-containing proteins usually exert important regulatory roles during multiple biological processes. TRIM8 has been demonstrated to be a RING domain-containing E3 ubiquitin ligase which plays critical roles in inflammation and cancer. In this study, a TRIM8 homolog from grouper, Epinephelus coioides (EcTRIM8) was cloned, and its effects on fish virus replication were investigated. The full-length EcTRIM8 cDNA encoded a polypeptide of 568 amino acids with 92% identity to TRIM8 homolog from large yellow croaker (Larimichthys crocea)...
July 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27109200/molecular-cloning-and-characterization-of-a-galectin-1-homolog-in-orange-spotted-grouper-epinephelus-coioides
#12
Xiuli Chen, Jingguang Wei, Meng Xu, Min Yang, Pingfei Li, Shina Wei, Youhua Huang, Qiwei Qin
As a member of animal lectin family, galectin has the functions of pathogen recognition, anti-bacteria and anti-virus. In the present study, a galectin-1 homolog (EcGel-1) from grouper (Epinephelus coioides) was cloned and its possible role in fish immunity was analyzed. The full length cDNA of EcGel-1 is 504 bp, including a 408 bp open reading frame (ORF) which encodes 135 amino acids with a molecular mass of 15.19 kDa. Quantitative real-time PCR analysis indicated that EcGel-1 was constitutively expressed in all analyzed tissues of healthy grouper...
July 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27050314/antiviral-function-of-grouper-mda5-against-iridovirus-and-nodavirus
#13
Youhua Huang, Yepin Yu, Ying Yang, Min Yang, Linli Zhou, Xiaohong Huang, Qiwei Qin
Melanoma differentiation-associated gene 5 (MDA5) is a critical member of retinoic acid-inducible gene I (RIG-I)-like receptor (RLR) family which can recognize viral RNA and enhances antiviral response in host cells. In this study, a MDA5 homolog from orange spotted grouper (Epinephelus coioides) (EcMDA5) was cloned, and its roles on grouper virus infection were characterized. The full-length EcMDA5 cDNA encoded a polypeptide of 982 amino acids with 74% identity with MDA5 homolog from rock bream (Oplegnathus fasciatus)...
July 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26945103/a-thioredoxin-domain-containing-protein-12-from-black-rockfish-sebastes-schlegelii-responses-to-immune-challenges-and-protection-from-apoptosis-against-oxidative-stress
#14
William Shanthakumar Thulasitha, Navaneethaiyer Umasuthan, R G P T Jayasooriya, Jae Koo Noh, Hae-Chul Park, Jehee Lee
Thioredoxin (TXN) superfamily proteins are identified by the presence of a thioredoxin active site with a conserved CXXC active motif. TXN members are involved in a wide range of biochemical and biological functions including redox regulation, refolding of disulfide containing proteins, and regulation of transcription factors. In the present study, a thioredoxin domain-containing protein 12 was identified and characterized from black rockfish, Sebastes schlegelii (RfTXNDC12). The full length of RfTXNDC12 consists of a 522-bp coding region encoding a 173-amino acid protein...
July 2016: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/26784918/fish-trim39-regulates-cell-cycle-progression-and-exerts-its-antiviral-function-against-iridovirus-and-nodavirus
#15
Wei Wang, Youhua Huang, Yepin Yu, Ying Yang, Meng Xu, Xiuli Chen, Songwei Ni, Qiwei Qin, Xiaohong Huang
The tripartite motif (TRIM)-containing proteins exert important immune regulatory roles through regulating different signaling pathways in response to different stimuli. TRIM39, a member of the TRIM family, is a RING domain-containing E3 ubiquitin ligase which could regulate cell cycle progression and apoptosis. However, the antiviral activity of TRIM39 is not explored. Here, a TRIM39 homolog from grouper, Epinephelus coioides (EcTRIM39) was cloned, and its effects on cell cycle progression and fish virus replication were investigated...
March 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26691306/molecular-clone-and-characterization-of-c-jun-n-terminal-kinases-2-from-orange-spotted-grouper-epinephelus-coioides
#16
Minglan Guo, Jingguang Wei, Yongcan Zhou, Qiwei Qin
c-Jun N-terminal kinase 2 (JNK2) is a multifunctional mitogen-activated protein kinases involving in cell differentiation and proliferation, apoptosis, immune response and inflammatory conditions. In this study, we reported a new JNK2 (Ec-JNK2) derived from orange-spotted grouper, Epinephelus coioides. The full-length cDNA of Ec-JNK2 was 1920 bp in size, containing a 174 bp 5'-untranslated region (UTR), 483 bp 3'-UTR, and a 1263 bp open reading frame (ORF), which encoded a putative protein of 420 amino acids...
February 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26631805/down-regulation-of-cd53-expression-in-epinephelus-coioides-under-lps-poly-i-c-and-cytokine-stimulation
#17
Chia-Yi Hou, John Han-You Lin, Shih-Jie Lin, Wan-Ching Kuo, Han-Tso Lin
Tetraspanins are a group of cell surface molecules involved in cell adhesion, motility, metastasis, signal transduction, and immune cell activation. Members of the tetraspanin family include CD9, CD37, CD63, CD53, and others. However, few tetraspanins have been investigated in teleosts. In this study, we obtained the open reading frame of CD53 cDNA from orange spotted grouper (Epinephelus coioices), an economically important fish. The predicted amino acid structure contains four membrane-spanning domains and a conserved CCG motif...
April 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26616235/molecular-cloning-and-expression-analysis-of-small-ubiquitin-like-modifier-sumo-genes-from-grouper-epinephelus-coioides
#18
Meng Xu, Jingguang Wei, Xiuli Chen, Pin Gao, Yongcan Zhou, Qiwei Qin
Small ubiquitin-like modifier (SUMO) is a group of proteins binding to lysine residues of target proteins and thereby modifying their stability, activity and subcellular localization. In the present study, two SUMO homolog genes (EcSUMO1 and EcSUMO2) from grouper (Epinephelus coioides) were cloned and characterized. The full-length sequence of EcSUMO1 was 749 bp in length and contained a predicted open reading frame of 306 bp encoding 101 amino acids with a molecular mass of 11.34 kDa. The full-length sequence of EcSUMO2 was 822 bp in length and contained a predicted open reading frame of 291 bp encoding 96 amino acids with a molecular mass of 10...
January 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26549177/molecular-cloning-and-characterization-of-orange-spotted-grouper-epinephelus-coioides-cxc-chemokine-ligand-12
#19
Chen-Shiou Wu, Ting-Yu Wang, Chin-Feng Liu, Hao-Ping Lin, Young-Mao Chen, Tzong-Yueh Chen
Chemokines are a family of soluble peptides that can recruit a wide range of immune cells to sites of infection and disease. The CXCL12 is a chemokine that binds to its cognate receptor CXCR4 and thus involved in multiple physiological and pathophysiological processes. In this study, we cloned and characterized CXCL12 from Epinephelus coioides (osgCXCL12). We found that the open reading frame of osgCXCL12 consists of 98 amino acid residues with the small cytokine C-X-C domain located between residues 29 and 87...
December 2015: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/26359551/antiviral-role-of-grouper-sting-against-iridovirus-infection
#20
Youhua Huang, Zhengliang Ouyang, Wei Wang, Yepin Yu, Pengfei Li, Sheng Zhou, Shina Wei, Jingguang Wei, Xiaohong Huang, Qiwei Qin
Stimulator of interferon genes (STING, also known as MITA, ERIS, MPYS or TMEM173) has been identified as a central component in the innate immune response to cytosolic DNA and RNA derived from different pathogens. However, the detailed role of STING during fish iridovirus infection still remained largely unknown. Here, the STING homolog from grouper Epinephelus coioides (EcSTING) was cloned and its effects on IFN response and antiviral activity were investigated. The full-length EcSTING cDNA was composed of 1590 bp and encoded a polypeptide of 409 amino acids with 80% identity to STING homolog from large yellow croaker...
November 2015: Fish & Shellfish Immunology
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