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Chen Shen, Wanli Zhao, Xuming Liu, Jihua Liu
OBJECTIVES: To investigate the regio-selective demethylation of papaverine by CYP105D1 and develop a whole-cell biocatalytic system for the preparative synthesis of 6-O-demethyl-papaverine. RESULTS: CYP105D1 from Streptomyces griseus ATCC 13273 was used for the regioselective demethylation of papaverine at C-6 using putidaredoxin reductase (PDR) and putidaredoxin (Pdx) as the electron transport system. The Km value of CYP105D1 towards papaverine was estimated to be 92...
November 21, 2018: Biotechnology Letters
(no author information available yet)
No abstract text is available yet for this article.
November 19, 2018: Proceedings of the National Academy of Sciences of the United States of America
Jong Soo Han, Su Jin Kim, Yoonjin Nam, Hak Yeong Lee, Geon Min Kim, Dong Min Kim, Uy Dong Sohn
Most diabetic patients experience diabetic mellitus (DM) urinary bladder dysfunction. A number of studies evaluate bladder smooth muscle contraction in DM. In this study, we evaluated the change of bladder smooth muscle contraction between normal rats and DM rats. Furthermore, we used pharmacological inhibitors to determine the differences in the signaling pathways between normal and DM rats. Rats in the DM group received an intraperitoneal injection of 65 mg/kg streptozotocin and measured blood glucose level after 14 days to confirm DM...
November 12, 2018: Biomolecules & Therapeutics
Jian-Lin Hu, Hui-Xing Chen, Hui-Rong Chen, Yu Wu, Xiao-Wen Sun, Zheng Li, Jin-Fang Xing
Structural alterations in fibroelastic components of the penile corpus cavernousum (CC) may impair its compliance, resulting in venous leakage and erectile dysfunction (ED). Our study evaluated the effectiveness of noninvasive two-dimensional shear-wave elastography (2-D SWE) in quantifying penile CC lesions in rabbits with hyperlipidemia-induced ED. A total of 12 New Zealand white rabbits were randomly divided into two groups. Six were fed a high-cholesterol diet containing 2% cholesterol and 8.5% lard for 10 weeks and the other six were fed normal diet as controls...
November 2, 2018: Asian Journal of Andrology
Ying Cheng, Zhi-Meng Wang, Weiqi Tan, Xiaona Wang, Yujing Li, Bing Bai, Yuxin Li, Shuang-Feng Zhang, Hai-Liang Yan, Zuo-Lun Chen, Chang-Mei Liu, Ting-Wei Mi, Shuting Xia, Zikai Zhou, An Liu, Gang-Bin Tang, Cong Liu, Zhi-Jie Dai, Ying-Ying Wang, Hong Wang, Xusheng Wang, Yunhee Kang, Li Lin, Zhenping Chen, Nina Xie, Qinmiao Sun, Wei Xie, Junmin Peng, Dahua Chen, Zhao-Qian Teng, Peng Jin
Genetic analyses have linked microRNA-137 (MIR137) to neuropsychiatric disorders, including schizophrenia and autism spectrum disorder. miR-137 plays important roles in neurogenesis and neuronal maturation, but the impact of miR-137 loss-of-function in vivo remains unclear. Here we show the complete loss of miR-137 in the mouse germline knockout or nervous system knockout (cKO) leads to postnatal lethality, while heterozygous germline knockout and cKO mice remain viable. Partial loss of miR-137 in heterozygous cKO mice results in dysregulated synaptic plasticity, repetitive behavior, and impaired learning and social behavior...
December 2018: Nature Neuroscience
Victor Curvello, Philip Pastor, Hugh Hekierski, William M Armstead
OBJECTIVE: Traumatic brain injury (TBI) is an important contributor to morbidity and mortality. Cerebral autoregulation is impaired after TBI, contributing to poor outcome. Extracellular signal-related kinase (ERK) mitogen activated protein kinase (MAPK) and ET-1 are upregulated and contribute to impairment of cerebral autoregulation and histopathology after porcine fluid percussion brain injury (FPI). Recent studies show that inhaled nitric oxide (iNO) prevents impairment of cerebral autoregulation and histopathology after FPI in pigs...
November 1, 2018: Neurocritical Care
Shuhei Yoshida, Isao Koshima, Ayano Sasaki, Yumio Fujioka, Shogo Nagamatsu, Kazunori Yokota, Shuji Yamashita, Mitsunobu Harima
Despite advances in supermicrosurgical techniques, the ability to anastomose vessels with a diameter of less than 0.2 mm remains limited. One of the reasons for this limitation is that the dilation methods currently available, such as inserting the tip of a microforceps into the lumen or topical application of a vasodilator such as papaverine hydrochloride or xylocaine spray, are not effective in very small vessels. To overcome this problem, we have developed a method whereby nylon monofilaments are placed inside the vessel lumen to act as a dilator...
October 9, 2018: Annals of Plastic Surgery
Sik Namgoong, Jong-Phil Yang, Seong Ho Jeong, Seung Kyu Han, Woo Kyung Kim, Eun Sang Dhong
Microvascular free flap transfer has become a prevailing surgery with a failure rate of <5%. However, pedicle thrombosis occurs more frequently than indicated by the failure rate. This difference is due to the successful salvage of failing flaps. During exploration, thrombi are often encountered at anastomotic sites, whereas these causes could not explain vascular compromise in other patients. Thus, we hypothesized that thrombogenic processes might occur at remote sites, specifically within microvessels of the transferred flap...
October 4, 2018: Journal of Plastic Surgery and Hand Surgery
Rachel K Smith, Rebecca J Stacey, Ed Bergström, Jane Thomas-Oates
A method has been developed for extracting poppy alkaloids from oily matrices, specifically lipid residues associated with archaeological ceramics. The protocol has been applied to fresh and artificially aged poppyseed oil and to residue from a Late Bronze Age Cypriot juglet in the collections of the British Museum. The juglet is of a type that has been linked with ancient trade in opium due to its poppy-head shape and wide distribution; it is a rare example of an intact vessel with contents sealed inside. Bulk analysis of the residue by GC-EI-MS and pyGC-EI-MS indicated a degraded plant oil and possible presence of papaverine...
October 3, 2018: Analyst
Szilvia Berkó, Stella Zsikó, Gábor Deák, Attila Gácsi, Anita Kovács, Mária Budai-Szűcs, László Pajor, Zoltán Bajory, Erzsébet Csányi
Purpose: Papaverine hydrochloride (PaHCl) is an old, well-known drug with spasmolytic activity but it has therapeutic effect in erectile dysfunction, too. As an intracavernous injection, it is not used in urologic clinics today because the side effects of the injection are pain, scarring or priapism. Our aim was to develop and test a topical semi-solid preparation containing PaHCl that would provide an alternative administration option by eliminating the undesirable side effects of the injection...
2018: Drug Design, Development and Therapy
Jonathan Hédouin, Cédric Schneider, Isabelle Gillaizeau, Christophe Hoarau
Palladium-catalyzed intramolecular carbopalladation onto allenamides completed by direct C-H allylation of heterocycles is studied. The domino construction/heteroarylation of isoquinolone process is first achieved. A general three-step one-pot strategy, involving in situ generation of allenamide, π-allyl-Pd complex generation, and interception with heteroarenes, has been subsequently set up. This methodology has been extended to the construction/heteroarylation of indoles, dihydroquinolines, isoquinolin(on)es, and medium-sized nitrogen heterocycles, which are known to be key challenging structural motifs with pharmaceutical significance...
October 5, 2018: Organic Letters
Y Lin, J-F He, X Zhang, H-M Wang
The aim of this study was to identify intraoperative factors associated with free flap failure. The outcomes of 216 patients (220 flaps) were analyzed retrospectively. A statistical analysis was performed to determine the association of flap failure with the intraoperative factors of prolonged operation time, flap type, vascular pedicle at the recipient site, and use of vasoactive medication. A review of the recent literature was also conducted to identify other intraoperative risk factors. Univariate regression analysis revealed that a prolonged operative time (P=0...
September 5, 2018: International Journal of Oral and Maxillofacial Surgery
Masahiro Shibata, Tomohiro Komine, Yuki Maeda, Hiroki Nakamura
Our previous studies demonstrated that vascular wall oxygen consumption rate (QO2 ) of arteriole under in vivo functional condition was dramatically greater than that reported by isolated vascular segment. Furthermore, such high QO2 of vascular wall decreases during vasodilation. However, it is unknown whether such a reduction in QO2 during vasodilation would be caused by a decrease in consumption of vascular endothelial cell (EC) or smooth muscle. In this study, we aimed to explain whether vascular EC or smooth muscle affects the decrease in vascular wall QO2 during vasodilation...
2018: Advances in Experimental Medicine and Biology
Seung-Hyun Jeong, Ji-Hun Jang, Seong-Ho Ham, Seung-Jeong Yang, Hea-Young Cho, Yong-Bok Lee
The purpose of this study was to develop a method for simultaneous analysis of schizandrin, ephedrine, paeoniflorin, and cinnamic acid as constituents of Socheongryong-tang tablet in human plasma using UPLC-MS/MS. These four components were separated using water containing 0.01% formic acid and methanol as a mobile phase by gradient elution at a flow rate of 0.3 mL/min with a HALO-C18 column (2.1 mm × 100 mm, 2.7 μm particle size). Quantitation was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique operated in multiple reaction monitoring mode...
September 15, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Arthur Hosmann, Steffen Rauscher, Wei-Te Wang, Philippe Dodier, Gerhard Bavinzski, Engelbert Knosp, Andreas Gruber
OBJECTIVE: After subarachnoid hemorrhage, delayed onset vasospasm can result in devastating ischemic stroke. The phenomenon of delayed cerebral ischemia (DCI) is not yet fully understood, and the correlation of angiographic vasospasm and cerebral infarction is still unclear. Therefore, we investigated the effect of endovascular treatment on the angiographic response and occurrence of DCI. METHODS: Eighty patients with subarachnoid hemorrhage and serious cerebral vasospasm underwent endovascular treatment using intra-arterial papaverine-hydrochloride (IAP) or transluminal balloon angioplasty (TBA)...
November 2018: World Neurosurgery
Hugh Hekierski, Philip Pastor, Victor Curvello, William M Armstead
Traumatic brain injury (TBI) contributes to morbidity in children, and boys are disproportionately represented. Cerebral blood flow (CBF) is reduced and autoregulation is impaired after TBI, contributing to poor outcome. Cerebral perfusion pressure (CPP) is often normalized by use of vasoactive agents to increase mean arterial pressure (MAP). In prior studies of male and female newborn and juvenile pigs, we observed that phenylephrine, norepinephrine, epinephrine, and dopamine demonstrated different sex- and age-dependent abilities to prevent impairment of cerebral autoregulation and limit histopathology after TBI, despite equivalent CPP values...
September 6, 2018: Journal of Neurotrauma
Chunyan Liu, Xiaohui Xu, Wenxin Hu, Xing Yang, Panpan Zhou, Guoyu Qiu, Weichun Ye, Yumin Li, Chaoyang Jiang
Clean cabbagelike (111) faceted silver crystals were synthesized via a facile galvanic replacement reaction of [Ag(NH3 )2 ]OH and a commercial aluminum foil, a surfactant-free formation process. The cabbagelike silver crystals consisted of interconnected nanoplates and exhibited a single-crystal structure along with preferential (111) facet oriented growth. These silver crystals showed high and reliable surface-enhanced Raman scattering (SERS) activity due to electromagnetic mechanism, and they could be easily transferred onto other rigid or flexible surfaces, making their SERS applications more versatile...
August 21, 2018: Analytical Chemistry
Federico Lo Torto, Juste Kaciulyte, Pedro Ciudad, Emanuele Cigna, Diego Ribuffo
No abstract text is available yet for this article.
September 2018: Plastic and Reconstructive Surgery
Bingbo Bao, Hongyi Zhu, Xianyou Zheng
No abstract text is available yet for this article.
September 2018: Plastic and Reconstructive Surgery
Adrienn Kazsoki, Attila Domján, Károly Süvegh, Romána Zelkó
Papaverine hydrochloride loaded gels, films and electrospun fibers were prepared for buccal drug delivery with the aim of improving the oral bioavailability of the crystalline drug, which can be achieved by the increased solubility and by the circumvention of the intensive first pass metabolism. The water soluble hydroxypropyl cellulose (HPC) was chosen as a mucoadhesive polymer. In order to improve the electrospinnability of HPC, the similarly mucoadhesive poly(vinyl alcohol) (PVA) was used. Since the drying of gels is of decisive role in either the formation of drug-loaded cast films or electrospun fibers, a real time ortho-positronium (o-Ps) tracking of gels was applied in order to obtain information about the supramolecular changes of the drying-induced gel-film transition...
September 15, 2018: European Journal of Pharmaceutical Sciences
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