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Maryam Musavi, Fatemeh Kohram, Mozhgan Abasi, Zohreh Bolandi, Mohammad Ajoudanian, Samira Mohammadi-Yeganeh, Seyed M Hashemi, Kazem Sharifi, Hamid Reza Fathi, Hossein Ghanbarian
Rn7SK is a conserved small nuclear noncoding RNA which its function in aging has not been studied. Recently, we have demonstrated that Rn7SK overexpression reduces cell viability and is significantly downregulated in stem cells, human tumor tissues, and cell lines. In this study, we analyzed the role of Rn7SK on senescence in adipose tissue-derived mesenchymal stem cells (AD-MSCs). For this purpose, Rn7SK expression was downregulated and upregulated via transfection and transduction, respectively, in AD-MSCs and subsequently, various distinct characteristics of senescence including cell viability, proliferation, colony formation, senescence-associated β galactosidase activity, and differentiation potency was analyzed...
January 13, 2019: Journal of Cellular Physiology
Atsuko Imai, Eka Fujimoto, Kaho Tamura, Masanori Utsuyama, Kazuto Sato
AIM: Maternal obesity and improper nutrition predispose offspring to chronic metabolic diseases. Although the frequency of these diseases increases with aging, the effect of a maternal high-fat diet on aged offspring remains elusive. MAIN METHODS: C57BL/6J female mice were fed a high-fat (HF) diet or a control (CON) diet and then mated. All offspring remained with their birth dam until weaning at 3 weeks. After weaning, the offspring from the HF and CON diet-fed dams were given either the HF diet or CON diet, which resulted in four groups: CON/CON, CON/HF, HF/CON, and HF/HF...
January 7, 2019: Life Sciences
Clara Lefranc, Malou Friederich-Persson, Laura Braud, Roberto Palacios-Ramirez, Susanne Karlsson, Nabiha Boujardine, Roberto Motterlini, Frederic Jaisser, Aurelie Nguyen Dinh Cat
Adipose tissue (AT) senescence and mitochondrial dysfunction are associated with obesity. Studies in obese patients and animals demonstrate that the MR (mineralocorticoid receptor) contributes to obesity-associated cardiovascular complications through its specific role in AT. However, underlying mechanisms remain unclear. This study aims to elucidate whether MR regulates mitochondrial function in obesity, resulting in AT premature aging and vascular dysfunction. Obese (db/db) and lean (db/+) mice were treated with an MR antagonist or a specific mitochondria-targeted antioxidant...
February 2019: Hypertension
Amiya Kumar Ghosh, Martin O'Brien, Theresa Mau, Nathan Qi, Raymond Yung
Visceral adipose tissue (VAT) inflammation plays a central role in longevity and multiple age-related disorders. Cellular Senescence (SEN) is a fundamental aging mechanism that contributes to age-related chronic inflammation and organ dysfunction, including VAT. Recent studies using heterochronic parabiosis models strongly suggested that circulating factors in young plasma alter the aging phenotypes of old animals. Our study investigated if young plasma rescued SEN phenotypes in the VAT of aging mice. With heterochronic parabiosis model using young (3 m) and old (18 m) mice, we found significant reduction in the levels pro-inflammatory cytokines and altered adipokine profile that are protective of SEN in the VAT of old mice...
December 26, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
Junfen Fan, Xingyan An, Yanlei Yang, Haoying Xu, Linyuan Fan, Luchan Deng, Tao Li, Xisheng Weng, Jianmin Zhang, Robert Chunhua Zhao
With the expansion of the elderly population, age-related osteoporosis and the resulting bone loss have become a significant health and socioeconomic issue. In Triple Energizer (TE)/San Jiao (SJ)/mesenchymal tissue system, mesenchymal stem cell (MSC) senescence, and impaired osteogenesis are thought to contribute to age-related diseases such as osteoporosis. Therefore, comprehending the molecular mechanisms underlying MSC senescence and osteogenesis is essential to improve the treatment of bone metabolic diseases...
December 2018: Aging and Disease
Kaho Takigawa, Rintaro Matsuda, Ran Uchitomi, Takumi Onishi, Yukino Hatazawa, Yasutomi Kamei
We examined the effect of long-term exercise on the prevention of sarcopenia using a senescence-accelerated-prone mice (SAMP8) model. Mice were housed in a wheel cage for 25 weeks to increase voluntary exercise. At week 23, endurance running capacity was examined using a treadmill. In a treadmill running test, the wheel cage group had increased endurance running capacity, which suggests that aging-related loss of muscle function was recovered by long-term exercise. Mice were sacrificed and microarray analysis revealed that genes involved in protein synthesis and degradation were upregulated in the skeletal muscles of the wheel cage group, suggesting accelerated protein turnover...
December 12, 2018: Bioscience, Biotechnology, and Biochemistry
Yulia A Panina, Anton S Yakimov, Yulia K Komleva, Andrey V Morgun, Olga L Lopatina, Natalia A Malinovskaya, Anton N Shuvaev, Vladimir V Salmin, Tatiana E Taranushenko, Alla B Salmina
Adipose tissue is recognized as an important organ with metabolic, regulatory, and plastic roles. Adipose tissue-derived stem cells (ASCs) with self-renewal properties localize in the stromal vascular fraction (SVF) being present in a vascular niche, thereby, contributing to local regulation of angiogenesis and vessel remodeling. In the past decades, ASCs have attracted much attention from biologists and bioengineers, particularly, because of their multilineage differentiation potential, strong proliferation, and migration abilities in vitro and high resistance to oxidative stress and senescence...
2018: Frontiers in Physiology
Weiwei Wang, Xun Xu, Zhengdong Li, Karl Kratz, Nan Ma, Andreas Lendlein
Non-swelling hydrophobic poly(n-butyl acrylate) network (cPnBA) is a candidate material for synthetic vascular grafts owing to its low toxicity and tailorable mechanical properties. Mesenchymal stem cells (MSCs) are an attractive cell type for accelerating endothelialization because of their superior anti-thrombosis and immune modulatory function. Further, they can differentiate into smooth muscle cells or endothelial-like cells and secret pro-angiogenic factors such as vascular endothelial growth factor (VEGF)...
December 3, 2018: Clinical Hemorheology and Microcirculation
Yung-Ming Chang, Marthandam Asokan Shibu, Chuan-Te Tsai, Chin-Chuan Tsai, Shu-Luan Lin, Chia-Cheng Chang, Vijaya Padma Viswanadha, Ray-Jade Chen, Julia Huei-Mei Chang, Chih-Yang Huang
Adipose-derived mesenchymal stem cells (ADMSCs) are easily accessible and are attractive mesenchymal stem cells for use in regenerative medicine; however their application is frequently restricted due to various challenges present in the environment they are administered. Therefore ADMSCs are preferably preconditioned with various stimulating factors to overcome the barriers developed in any pathological conditions. Here we used ADMSCs from rat adipose based on the abundance of positive markers and preconditioned the cells with extracts from Alpinate Oxyphyllae Fructus (AOF), a traditional Chinese herb used for antiaging, associated various health benefits...
December 4, 2018: Journal of Cellular Physiology
Agnieszka Baranowska-Bik, Wojciech Bik
Insulin was discovered in 1922 by Banting and Best. Since that time, extensive research on the mechanisms of insulin activity and action has continued. Currently, it is known that the role of insulin is much greater than simply regulating carbohydrate metabolism. Insulin in physiological concentration is also necessary to maintain normal vascular function. Insulin resistance is defined as a pathological condition characterized by reduced sensitivity of skeletal muscles, liver, and adipose tissue, to insulin and its downstream metabolic effects under normal serum glucose concentrations...
November 28, 2018: Current Vascular Pharmacology
José María Moreno-Navarrete, Laura Liñares-Pose, Mònica Sabater, Eva Rial-Pensado, Ferran Comas, Mariona Jové, Jèssica Latorre, Francisco Ortega, Wifredo Ricart, Manuel Portero-Otin, Miguel López, José Manuel Fernández-Real
BACKGROUND/AIMS: Thyroid hormones have been recently linked to senescence and longevity. Given the recent description of TSHB mRNA in human adipose tissue (AT), we aimed to investigate the relationship between local AT TSH and adipose tissue senescence. METHODS: TSHB mRNA (measured by real-time PCR) and markers of adipose tissue senescence [BAX, DBC1, TP53, TNF (real-time PCR), telomere length (Telo TAGGG Telomere Length Assay) and lipidomics (liquid chromatography mass spectrometry)] were analysed in subcutaneous (SAT) and visceral (VAT) AT from euthyroid subjects...
2018: Cellular Physiology and Biochemistry
Federica Facchin, Eva Bianconi, Miriam Romano, Alessia Impellizzeri, Francesco Alviano, Margherita Maioli, Silvia Canaider, Carlo Ventura
Human Mesenchymal Stem Cells (hMSCs) undergo senescence in lifespan. In most clinical trials, hMSCs experience long-term expansion ex vivo to increase cell number prior to transplantation, which unfortunately leads to cell senescence, hampering post-transplant outcomes. Hydrogen peroxide (H2 O2 ) in vitro represents a rapid, time and cost-effective tool, commonly used as oxidative stress tantalizing the stem cell ability to cope with a hostile environment, recapitulating the onset and progression of cellular senescence...
2018: International Journal of Medical Sciences
Behnaz Banimohammad Shotorbani, Helder André, Abolfazl Barzegar, Nosratollah Zarghami, Roya Salehi, Effat Alizadeh
Cell sheet technology aims at replacement of artificial extracellular matrix (ECM) or scaffolds, popular in tissue engineering, with natural cell derived ECM. Adipose tissue mesenchymal stem cells (ASCs) have the ability of ECM secretion and presented promising outcomes in clinical trials. As well, different studies found that secretome of ASCs could be suitable for triggering cell free regeneration induction. The aim of this study was to investigate the effect of using two bio-factors: secretome of ASCs (SE) and vitamin C (VC) for cell sheet engineering on a thermosensitive poly N-isopropyl acryl amide-Methacrylic acid (P(NIPAAm-MAA)) hydrogel...
November 3, 2018: Journal of Materials Science. Materials in Medicine
Zhao Wei, Haiyang Guo, Junchao Qin, Shihua Lu, Qiao Liu, Xiyu Zhang, Yongxin Zou, Yaoqin Gong, Changshun Shao
Cellular senescence, an irreversible proliferative arrest, functions in tissue remodeling during development and is implicated in multiple aging-associated diseases. While senescent cells often manifest an array of senescence-associated phenotypes, such as cell cycle arrest, altered heterochromatin architecture, reprogrammed metabolism and senescence-associated secretory phenotype (SASP), the identification of senescence cells has been hindered by lack of specific and universal biomarkers. To systematically identify universal biomarkers of cellular senescence, we integrated multiple transcriptome data sets of senescent cells obtained through different in vitro manipulation modes as well as age-related gene expression data of human tissues...
November 2, 2018: Free Radical Biology & Medicine
Yong-Ho Lee, So Ra Kim, Dai Hoon Han, Hee Tae Yu, Yoon Dae Han, Jin Hee Kim, Soo Hyun Kim, Chan Joo Lee, Byoung-Hoon Min, Dong-Hyun Kim, Kyung Hwan Kim, Jin Won Cho, Won-Woo Lee, Eui-Cheol Shin, Sungha Park
Senescent T cells have been implicated in chronic inflammatory and cardiovascular diseases. Here, we explored the relationship between senescent T cells and glycemic status in a cohort of 805 participants by investigating the frequency of CD57+ or CD28null senescent T cells in peripheral blood. Normal glucose tolerance (NGT) participants with follow-up data (N = 149) were included to determine whether hyperglycemia (prediabetes or type 2 diabetes) developed during follow-up (mean 2.3 years). CD8+ CD57+ and CD8+ CD28null T cell frequencies were significantly higher in prediabetes and type 2 diabetes compared with NGT...
November 2, 2018: Diabetes
Chiara Fiorillo, Maria Rosaria D'Apice, Federica Trucco, Michela Murdocca, Paola Spitalieri, Stefania Assereto, Serena Baratto, Guido Morcaldi, Carlo Minetti, Federica Sangiuolo, Giuseppe Novelli
Mandibular hypoplasia, deafness, and progeroid features, with concomitant lipodystrophy, define a multisystem disorder named MDPL syndrome. MDPL has been associated with heterozygous mutations in POLD1 gene resulting in loss of DNA polymerase δ activity. In this study, we report clinical, genetic, and cellular studies of a 13-year-old Pakistani girl, presenting growth retardation, sensorineural deafness, altered distribution of subcutaneous adipose tissue, and insulin resistance. We performed Sanger sequencing of POLD1 gene in the proband and the healthy parents...
November 2, 2018: DNA and Cell Biology
Katarzyna Kornicka, Jolanta Szłapka-Kosarzewska, Agnieszka Śmieszek, Krzysztof Marycz
Obesity and endocrine disorders have become prevalent issues in the field of both human and veterinary medicine. Equine metabolic syndrome is a complex disorder involving alternation in metabolism and chronic systemic inflammation. It has been shown that unfavourable microenvironment of inflamed adipose tissue negatively affects adipose stem cell population (ASC) residing within, markedly limiting their therapeutic potential. ASCsEMS are characterized by increased senescence apoptosis, excessive accumulation of reactive oxygen species (ROS), mitochondria deterioration and "autophagic flux...
October 28, 2018: Journal of Cellular and Molecular Medicine
Jia Fang, Yuan Yan, Xin Teng, Xinyu Wen, Na Li, Sha Peng, Wenshuai Liu, F Xavier Donadeu, Shanting Zhao, Jinlian Hua
Transplantation of adipose-derived mesenchymal stem cells (ADMSCs) can aid in the treatment of numerous diseases in animals. However, natural aging during in vitro expansion of ADMSCs prior to their use in transplantation restricts their beneficial effects. Melatonin is reported to exert biorhythm regulation, anti-oxidation, and anti-senescence effects in various animal and cell models. Herein, by using a senescent canine ADMSCs (cADMSCs) cell model subjected to multiple passages in vitro , we investigated the effects of melatonin on ADMSCs senescence...
October 25, 2018: Aging
Radhakrishnan Vishnubalaji, Muthurangan Manikandan, Abdullah Aldahmash, Abdullah AlJarbou, Mohamad Habous, Dulaim Alhajeri, Raed Almannie, Musaad Alfayez, Nehad M Alajez, Saleh Binsaleh
BACKGROUND: Stem cell-based therapies have recently been explored in the field of erectile dysfunction (ED). However, the cellular and molecular phenotype of adipose derived stem cells (ADSCs) stromal vascular fraction (SVF) from ED patients remains largely unknown. Herein we compared the global gene expression profile in the SVF from ED patients and healthy individuals and identified altered signaling pathways between the two groups. METHODS: Samples (2-5g) of abdominal adipose tissue from ED patients (n=6) and healthy individual controls (n=3) undergoing elective cosmetic liposuction were collected...
October 17, 2018: Bioscience Reports
Abbas Ishaq, Damien Dufour, Kerry Cameron, Thomas von Zglinicki, Gabriele Saretzki
Dietary restriction (DR) is thought to exert its beneficial effects on healthspan at least partially by a senolytic and senostatic action, i.e. by reducing frequencies of cells with markers of DNA damage and senescence in multiple tissues. Due to its importance in metabolic and inflammation regulation, fat is a prime tissue for health span determination as well as a prime target for DR. We aimed to determine here whether the beneficial effects of DR would be retained over a subsequent period of ad libitum (AL) feeding...
November 2018: Experimental Gerontology
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