Read by QxMD icon Read

Electrochemical cancer

Kazuharu Sugawara, Hideki Kuramitz, Toshihiko Kadoya
We used an electron-transfer carbohydrate-mimetic peptide (YYYYC) to construct an electrochemical cytosensing system. Magnetic beads were modified with either asialofetuin (ASF) or soybean agglutinin (SBA) to evaluate the effect on cell sensing. Because SBA binds to the galactose residue that exists at the terminals of the carbohydrate chains in ASF, the target protein was accumulated on the protein magnetic beads. SBA is an example of N-acetylgalactosamine- and galactose-binding proteins that readily combine with YYYYC...
December 21, 2018: Analytica Chimica Acta
Elif Burcu Aydın, Mustafa Kemal Sezgintürk
In this study, we constructed a new and sensitive ITO based electrochemical immunosensor for detection of interleukin 1β (IL-1β), a cancer biomarker found in serum and saliva. 6-phosphonohexanoic acid (PHA) was used as a biomolecule immobilization matrix for the first time. Anti-IL-1β antibody was utilized as a biorecognition molecule that immobilized onto carboxyl groups of 6-phosphohexanoic acid (PHA) via amide bond. Selective interaction between anti- IL-1β antibodies and IL-1β antigens was investigated by Electrochemical Impedance Spectroscopy (EIS), Cyclic Voltammetry (CV) and Single Frequency Impedance (SFI) methods...
December 18, 2018: Analytica Chimica Acta
Julia Marzioch, Jochen Kieninger, Andreas Weltin, Hubert Flamm, Kuppusamy Aravindalochanan, Joe A Sandvik, Erik O Pettersen, Qian Peng, Gerald A Urban
We introduce a new system which combines metabolic monitoring using electrochemical microsensors with photodynamic therapy on-chip for the first time. Oxygen consumption of T-47D breast cancer cells was measured during therapy with protoporphyrin IX. We determined the efficacy of the therapy and revealed its recovery effects, which underlines the high relevance of continuous monitoring.
October 12, 2018: Lab on a Chip
Nan Zhou, Fangfang Su, Chuanpan Guo, Linghao He, Zhankui Jia, Minghua Wang, Qiaojuan Jia, Zhihong Zhang, Siyu Lu
Fabricating novel bimetallic metal organic framework (MOF) architectures and exploiting them as aptasensor scaffolds for detecting diverse analytes, especially cancer markers, have aroused widespread research attention. Herein, we report a novel strategy for obtaining ZnZr bimetallic MOFs via the MOF-on-MOF method and exploit them as an aptasensor platform for detecting the cancer marker protein tyrosine kinase-7 (PTK7). Basic characterizations reveal that the chemical structure, crystalline properties, and surface functionality of bimetallic ZnZr-MOFs can be modulated by changing the order of addition of metal precursors and organic ligands...
September 22, 2018: Biosensors & Bioelectronics
Azam Akbarnia, Hamid R Zare
The authors describe a DNA based voltammetric assay for the cancer biomarker microRNA-25. A glassy carbon electrode (GCE) was modified with amino-functionalized graphene quantum dots and used as an amplifier of electrochemical signals. p-Biphenol is introduced as a new electroactive probe with a fairly low working potential of 0.3 V (vs. Ag/AgCl). The stages of fabricating the electrode were characterized by cyclic voltammetry and electrochemical impedance spectroscopy. ss-Probe DNA was immobilized on the modified GCE and then exposed to a sample containing microRNA-25...
October 9, 2018: Mikrochimica Acta
Huifang Zhao, Yanqiu Li, Jie Wang, Miao Cheng, Zheng Zhao, Huinian Zhang, Congwei Wang, Junying Wang, Yan Qiao, Junzhong Wang
It is challenging to realize a dual-ion mode of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for detecting small molecules. Herein, graphene coated by porous amorphous carbon with P-O surface group and codoped by phosphorus and nitrogen (O-P,N-C/G) was synthesized from an aerogel formed by phytic acid, polyaniline, and electrochemically exfoliated graphene. The carbon material synthesized has the feature of large surface area (583 m2 /g), good electrical conductivity, strong UV absorption, heteroatom doping, and surface functional groups suitable for laser- induced desorption/ionization...
October 17, 2018: ACS Applied Materials & Interfaces
Gayatri S Phadke, Jennifer E Satterwhite-Warden, Dharamainder Choudhary, John A Taylor, James F Rusling
We report a low-cost, sensitive, bead-based electrochemical immunoarray for soluble L-selectin (or CD62L protein), a potential biomarker for staging bladder cancer. We used a semi-automated modular microfluidic array with online antigen capture on superparamagnetic beads, which were subsequently delivered to a detection chamber housing multiple sensors. The assay was designed to accurately detect CD62L in diluted serum with a limit of detection (LOD) of 0.25 ng mL-1 and a dynamic range of 0.25-100 ng mL-1. The microfluidic array gave significantly better accuracy and higher sensitivity than a standard ELISA kit, which was shown to be subject to significant systematic error at high and low concentration ranges...
October 8, 2018: Analyst
Mohammad Hasanzadeh, Arezoo Mohammadzadeh, Mohsen Jafari, Biuck Habibi
CA 125 is a Mucin glycoprotein and its concentration in human serum correlates with a woman's risk of developing ovarian cancer and also indicates response to therapy in diagnosed patients. Accurate detection of this large, complex protein in patient biofluids is of great clinical relevance. In this work, an innovative immunoassay for quantitation of CA 125 based on signal amplification strategy was proposed. In this work, Horseradish peroxidase (HRP) labeled CA 125-antibody (anti-CA 125) was immobilized onto a green and biocompatible nanocomposite containing poly cetyl trimethyl ammonium bromide (P (CTAB) as conductive matrix, chitosan (CS) as biocompatible agent and sliver nanoparticles (Ag NPs) as signal amplification element...
October 1, 2018: International Journal of Biological Macromolecules
Dawei Yang, Wenbo Cheng, Xifeng Chen, Yuguo Tang, Peng Miao
miRNAs are novel diagnostic and prognostic biomarkers for a diversity of diseases like cancers. In this study, an ultrasensitive electrochemical biosensor for miRNA evaluation is fabricated. A methylene blue (MB) labeled DNA probe is firstly modified on a gold electrode. By employing target induced strand displacement amplification and subsequent DNAzyme cleavage cycles, a large number of MB molecules are released. The decreased oxidation peak current could be used to reveal the miRNA concentration. Based on the two efficient signal amplifications, this method shows ultrahigh sensitivity...
October 4, 2018: Analyst
Brunna Xavier Martins, Raul Ferraz Arruda, Gildeíde Aparecida Costa, Hassan Jerdy, Sávio Bastos de Souza, Julianna Maria Santos, William Rodrigues de Freitas, Milton Masahiko Kanashiro, Eulógio Carlos Queiroz de Carvalho, Nadir Francisca Sant'Anna, Fernanda Antunes, Raul Martinez-Zaguilan, Sennoune Souad, Anna Lvovna Okorokova-Façanha, Arnoldo Rocha Façanha
BACKGROUND: Metastatic tumor cells have acidic extracellular pH and differential electrochemical H+ gradients generated across their cell membranes by V-type H+ -ATPases. This study shows that inhibition of the V-ATPases by the plant-derived monoterpene Myrtenal results in tumor cell death and decreased metastatic dissemination in mice. METHODS: The Myrtenal anticancer toxicity was evaluated in vitro using murine (B16F0 and B16F10) and human (SkMel-5) melanoma cell lines, and in in vivo mouse metastatic dissemination model...
September 11, 2018: Biochimica et biophysica acta. General subjects
Baoting Dou, Jin Li, Bingying Jiang, Ruo Yuan, Yun Xiang
The detection of single nucleotide polymorphisms (SNPs) is of great clinical significance to the diagnosis of various genetic diseases and cancers. In this work, the development of an ultrasensitive ratiometric electrochemical sensor for screening SNP with a significantly enhanced discrimination factor is reported. The ferrocene (Fc) and methylene blue (MB) dual-tagged triple helix complex (THC) probes are self-assembled on the gold electrode to construct the sensing interface. The addition of the mutant p53 gene causes the disassembly of the THC probes with the release of the Fc-tagged sequence and the folding of the MB-labeled sequence into a hairpin structure, causing the change in the current response ratio of MB to Fc for monitoring the mutant p53 gene...
December 14, 2018: Analytica Chimica Acta
Xuan Cai, Kaicha Chen, Zhenxing Wang, Wenqian Sun, Hongli Zhao, Huanhuan Zhang, Huilan Chen, Minbo Lan
The detection of superoxide anions (O2 •- ) is widely considered as a potential way for cancer diagnosis and the development of enzyme-mimic catalysts is the main challenge in the establishment of electrochemical sensors for O2 •- sensing in real samples. Here we present a novel enzyme- and metal-free electrochemical catalyst for superoxide (O2 •- ) sensing based on the widely-used carbon nanotubes (CNT). Through a one-step hydrothermal process induced by phytic acid (PA), CNT-based porous foam (PACNTF) was successfully obtained...
December 14, 2018: Analytica Chimica Acta
Ozge Er, Ece Eksin, Hale Melis Soylu, Bayram Göçmen, Ayşe Nalbantsoy, Fatma Yurt, Arzum Erdem
Snake venoms are a natural biological source that has potential therapeutic value with various protein compounds. Disintegrins originally were discovered as a family of proteins from snake venoms composed of cysteine rich low molecular weight polypeptides. Disintegrins exhibit specific binding and higher affinity toward integrin with potential inhibition of function. Trans-membrane receptors of the integrin family may involve in many pathological conditions such as inflammation and tumor progression with important processes related to invasion and migration...
October 1, 2018: Applied Biochemistry and Biotechnology
Danielle Aucamp, Sreedhar V Kumar, David C Liles, Manuel A Fernandes, Leonie Harmse, Daniela I Bezuidenhout
1,2,3-Triazol-5-ylidene (trz) complexes of gold(i) containing a ferrocenyl substituent on the C4-position of the trz ring were synthesized to yield the neutral heterobimetallic gold(i) trz chlorido (2), gold(i) trz phenyl (3), and the cationic gold(i) trz triphenylphospine (5) complexes. In order to compare the effect of silver(i) as central metal vs. gold(i), [Ag(trz)2]+ (4) was also prepared, while variation of the C4-1,2,3-triazol-5-ylidene substituent from a ferrocenyl to a phenyl group was done to prepare the monometallic analogue of 5, namely the cationic Au(i) trz triphenylphosphine complex 6...
October 2, 2018: Dalton Transactions: An International Journal of Inorganic Chemistry
Hua-Feng Wang, Rong-Na Ma, Fei Sun, Li-Ping Jia, Wei Zhang, Lei Shang, Qing-Wang Xue, Wen-Li Jia, Huai-Sheng Wang
A versatile label-free electrochemical biosensor based on dual enzyme assisted multiple amplification strategy was developed for ultrasensitive detection of circulating tumor DNA (ctDNA). The biosensor consists of a triple-helix molecular switch (THMS) as molecular recognition and signal transduction probe, ribonuclease HII (RNase HII) and terminal deoxynucleotidyl transferase (TdT) as dual enzyme assisted multiple amplification accelerator. The presence of target ctDNA could open THMS and trigger RNase HII-assisted homogenous target recycling amplification to produce substantial signal transduction probe (STP)...
December 30, 2018: Biosensors & Bioelectronics
Yuhan Yang, Yongyao Fu, Huilan Su, Li Mao, Mei Chen
The simple, rapid, sensitive, and specific detection of cancer cells plays a pivotal role in the diagnosis and prognosis of cancer. We developed a novel DNA-labeled sandwich electrochemical biosensor based on a glassy carbon electrode modified with 3D graphene and a hybrid of Au nanocages (Au NCs)/amino-functionalized multiwalled carbon nanotubes (MWCNT-NH2 ) for label-free and selective detection of MCF-7 breast cancer cells via differential pulse voltammetry. The layer-by-layer assembly and cell-detection performance of the Au NCs/MWCNTs-NH2 -based biosensor were investigated using scanning electron microscopy and electrochemical methods including cyclic voltammetry and electrochemical impedance spectroscopy...
December 30, 2018: Biosensors & Bioelectronics
Huiping Sun, Huan Wang, Wanqiao Bai, Lin Bao, Jianxun Lin, Yan Li
5-Hydroxymethylcytosine (5-hmC), an oxidation product of 5-mC (5-methylcytosine), is presented in DNA of most mammalian cells and play an important role in the alteration of cancer-related genes. Herein, a sensitive electrogenerated chemiluminescence (ECL) biosensing method for the determination of 5-hmC in DNA (5-hmC DNA) was established on the basis of chemical modification and nanomaterial amplification. First, electrochemically reduced molybdenum disulfide-poly(acrylic acid) (rMoS2 -PAA) nanosheets were used to modify glassy carbon electrode (GCE) to form an ECL biosensing electrode (rMoS2 -PAA/GCE) which has large accessible surface area to immobilize more DNA...
January 1, 2019: Talanta
Dongping Long, Chengchi Chen, Chenyu Cui, Zheng Yao, Peihui Yang
A single-cell sensor with a spatial architecture was firstly fabricated for realizing high precision single-cell analysis using an 11-mercaptoundecanoic acid (MUA)-spaced sensing interface to prop up single cells and provide a suitable space for effective nanoprobe labeling. Mercapto acids (MA) with different carbon chain lengths were optimized and MUA was selected to provide optimal interspace on the electrodeposited PANI/AuNP substrates, and its carboxyl could couple with folic acid to capture cancer cells...
September 27, 2018: Nanoscale
Panpan Sun, Wei-Wei Xiong, Dong Zhu, Zhuhua Dong, Xuping Jin, Beibei Liu, Yi Zhang, Beihua Bao, Weifeng Yao, Li Zhang, Fang-Fang Cheng
Facile and sensitive detection methods of cancer cells in the early stage are beneficial for monitoring cancers and treating patients in time to reduce the death rate. In this work, an ultrasensitive cytosensor was constructed using aptamers as cell capturers and metal ion-exchanged titanium phosphate nanospheres as electrochemical probes. KH1C12 can specifically recognize HL-60 cells and distinguish them from other cell lines, K562 and CCRF-CEM, to obtain high selectivity. Cadmium ion functionalized titanium phosphate nanospheres show large quantities of electroactive cadmium ion output and a highly sensitive electrochemical signal...
September 27, 2018: Analyst
Fenghe Duan, Shuai Zhang, Longyu Yang, Zhihong Zhang, Linghao He, Minghua Wang
A novel nanostructured biosensing platform was designed based on two-dimensional (2D) nanocomposite of graphitic carbon nitride (g-C3 N4 ) nanosheets and MoS2 quantum dots (MoS2 QDs), followed by decoration with chitosan-stabilized Au nanoparticles (CS-AuNPs) (denoted as MoS2 QDs@g-C3 N4 @CS-AuNPs), of which CS-AuNPs were prepared by plasma enhanced-chemical vapor deposition. Owning to the good surface plasmon performance of the CS-AuNPs and excellent electrochemical activity of MoS2 QDs@g-C3 N4 nanosheets, the as-obtained 2D MoS2 QDs@g-C3 N4 @CS-AuNPs nanocomposite was simultaneously explored to construct both surface plasmon resonance spectroscopy (SPR) sensor and electrochemical aptasensor...
December 7, 2018: Analytica Chimica Acta
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"