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Electrochemical cancer

Dimithree Kahanda, Naveen Singh, David A Boothman, Jason D Slinker
There is a great need to track the selectivity of anticancer drug activity and to understand the mechanisms of associated biological activity. Here we focus our studies on the specific NQO1 bioactivatable drug, ß-lapachone, which is in several Phase I clinical trials to treat human non-small cell lung, pancreatic and breast cancers. Multi-electrode chips with electrochemically-active DNA monolayers are used to track anticancer drug activity in cellular lysates and correlate cell death activity with DNA damage...
July 30, 2018: Biosensors & Bioelectronics
Reza Karimi Shervedani, Marzieh Samiei Foroushani, Amirhosein Kefayat, Mostafa Torabi, Fatemeh Rahnemaye Rahsepar
Construction of hybrid systems that combine the cancer treatment and diagnosis agents on a single platform, known as theranostic systems, have received great attentions in the field of nanobiomedicine. Here, construction and characterization of a new multifunctional hybrid theranostic system based on RGO, PDA, BSA, DTPA-Mn(II), and MTX constituents, is presented. Accordingly, GO is partially reduced and simultaneously functionalized by dopamine, leading to reduced graphene oxide/polydopamine, RGO-PDA system; and then, the bovine serum albumin protein (BSA) is grafted onto this system...
October 15, 2018: Biosensors & Bioelectronics
Saleheh Abbasi, Sajjad Gharaghani, Ali Benvidi, Masoud Rezaeinasab, Ali Akbar Saboury
Xanthine oxidase (XO) can catalyze xanthine to uric acid and has also been linked with the extension of some serious diseases such as cancer, gout, diabetes and so on. Thymol is a part of diet in the form of spices. Due to the high antioxidant activity, its inhibitory effect on XO was studied in the present work. XO organized in four redox domains which exhibiting electrochemical signals. Therefore, voltammetric methods can be used to obtain the valuable information about the action mechanism of thymol on XO...
August 7, 2018: International Journal of Biological Macromolecules
Samira Yazdanparast, Ali Benvidi, Maryam Banaei, Habib Nikukar, Marzieh Dehghan Tezerjani, Mostafa Azimzadeh
This paper reports on a sensitive and selective method for the detection of Michigan Cancer Foundation-7 (MCF-7) human breast cancer cells and MUC1 biomarker by using an aptamer-based sandwich assay. A biocompatible nanocomposite consisting of multiwall carbon nanotubes (MWCNT) and poly(glutamic acid) is placed on a glassy carbon electrode (GCE). The sandwich assay relies on the use of a mucin 1 (MUC1)-binding aptamer that is first immobilized on the surface of modified GCE. Another aptamer (labeled with silver nanoparticles) is applied for secondary recognition of MCF-7 cells in order to increase selectivity and produce an amplified signal...
August 9, 2018: Mikrochimica Acta
Wenhao Dong, Yipeng Ren, Zhixue Bai, Yi Yang, Zihua Wang, Cong Zhang, Qiang Chen
Recently, great efforts have been made to use biosensors for the early diagnosis of cancer. Specifically, using a biomarker to detect H2 O2 in physiological conditions is of great significance for understanding the signal transduction pathways and achieving early cancer diagnosis. In this work, we report an innovative H2 O2 sensor that was fabricated by trimetallic AuPtPd nanocomposites platform on reduced graphene oxide (rGO) nanosheets with the modification of the rGO and trimetallic AuPtPd nanoparticles on a glassy carbon electrode (GCE) by physical adsorption...
November 1, 2018: Talanta
Ammar Ebrahimi, Iraj Nikokar, Mehrdad Zokaei, Elahe Bozorgzadeh
The new sensitive diagnostic method, electrochemical nanobiosensor, is applied to cancer detection by using specific biomarkers such as miRNA. Studies have shown that for the diagnosis of Triple Negative Breast Cancer (TNBC) patients, in the early stages of the disease, miR-199a-5p as circulating miRNA can be a suitable candidate. In this study, a new electrochemical nanobiosensor for serum miR-199a-5p detection was designed by using modified glassy carbon electrode (GCE) with graphene oxide (GO) and gold nanoroad (GNR) on which a thiolated probe was immobilized...
November 1, 2018: Talanta
Mohammad Hasanzadeh, Rahimeh Sahmani, Elham Solhi, Ahad Mokhtarzadeh, Nasrin Shadjou, Soltanali Mahboob
Ovarian cancer, as one of the most life-threatening malignancies among women worldwide, is usually diagnosed at the late stage despite up regulation of molecular markers such as carcinoma antigen 125 (CA 125) at the early stages of the malignancy. CA 125 is the only tumor marker recommended for clinical use in the diagnosis and management of ovarian cancer. The potential role of CA-125 for the early detection of ovarian cancer is controversial and has not yet been adopted for widespread screening efforts in asymptomatic women...
August 3, 2018: International Journal of Biological Macromolecules
Guangli Wang, Jagotamoy Das, Sharif U Ahmed, Carine R Nemr, Libing Zhang, Mahla Poudineh, Edward H Sargent, Shana O Kelley
The development of new tools for tracking the activity of human DNA methyltransferases is an important goal given the role of this enzyme as a cancer biomarker and epigenetic modulator. However, analysis of the human DNA (cytosine-5)-methyltransferase 1 (Dnmt1) activity is challenging - especially in crude samples - because of the low activity and large size of the enzyme. Here, we report a new approach to Dnmt analysis that combines nanostructured electrodes with a digest-and-amplify strategy that directly monitors Dnmt1 activity with high sensitivity...
August 6, 2018: ACS Sensors
Hongxia Dai, Yonglei Chen, Xiaoying Niu, Congjie Pan, HongLi Chen, Xingguo Chen
An ingenious and viable method for preparing heterogeneous Co3 O4 dodecahedrons that contain carbon and encapsulated Au nanoparticles (Au@C-Co3 O4 ) was proposed via pyrolysis of Au nanoparticle-encapsulated zeolitic imidazolate framework-67 (Au@ZIF-67). The obtained Au@C-Co3 O4 possessed hierarchically porous structure, abundant active sites, excellent conductivity, and durability, all of which can significantly improve the analysis performance of the biosensor. Meanwhile, the fabricated electrode based on the porous Au@C-Co3 O4 showed remarkable electrocatalytic performance towards H2 O2 with a limit of detection of 19 nM and ultra-high sensitivity of 7553 μA mM-1 cm-2 , even when the Au content in the composite was as low as 0...
July 12, 2018: Biosensors & Bioelectronics
Payal Gulati, Prabhjot Kaur, M V Rajam, Tapasya Srivastava, Prabhash Mishra, S S Islam
A label-free electrochemical immunosensor is fabricated using high quality single-walled carbon nanotube for early detection of leukemia cells. It is based on P-glycoprotein (P-gp) expression level detection; by effective surface immune-complex formation with the monoclonal anti-P-glycoprotein antibodies bound to an epoxy modified nanotube surface. The expression level of P-gp on the leukemia cell surface detected by cyclic voltammetry is in good agreement with immunofluorescence microscopy studies. The proposed biosensor could be used for the detection of P-gp expressing cells within a linear range of 1...
July 23, 2018: Analytical Biochemistry
Zohreh Rezaeifar, Gholam Hossein Rounaghi, Zarrin Es'haghi, Mahmoud Chamsaz
Flutamide (FLT) is a non-steroidal anti-androgen drug that has a specific anti-androgenic activity so that it is used in the treatment of prostate cancer. FLT may also be used to treat excess androgen levels in women. A sensitive electrochemical sensor based on hyperbranchedpolyglycerol functionalized- graphene oxide developed, using ionic liquid mediated hollow fiber-pencil graphite electrode (HF/HBP-GO/PGE) as a working electrode for determination of an anticancer drug, flutamide (FLT. In this design, a two centimeter piece of porous polypropylene hollow fiber membrane was impregnated with ionic liquid (1-Pentyl-3-methylimidazoliumbromide), and a graphite rod modified with hyperbranchedpolyglycerol/graphene oxide (HBP-GO), was located inside the fiber lumen...
October 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Alejandro Valverde, Eloy Povedano, Víctor Ruiz-Valdepeñas Montiel, Paloma Yáñez-Sedeño, María Garranzo-Asensio, Rodrigo Barderas, Susana Campuzano, José M Pingarrón
This work describes the first electrochemical immunosensor reported for the determination of IL-13 receptor Rα2 (IL-13Rα2), an emerging relevant biomarker in metastatic colon cancer. The approach involves the formation of sandwich immunocomplexes using specific capture (CAb) and biotinylated detector antibodies (BDAb) further labeled with an streptavidin-horseradish peroxidase (Strep-HRP) polymer, onto carboxylic acid-modified magnetic microbeads (HOOC-MBs). Amperometric detection at disposable carbon screen-printed electrodes (SPCEs) using the (H2 O2 )/hydroquinone (HQ) system was employed to monitor the affinity reactions...
October 15, 2018: Biosensors & Bioelectronics
Laís C Brazaca, Camila B Bramorski, Juliana Cancino-Bernardi, Sanseray da Silveira Cruz-Machado, Regina P Markus, Bruno C Janegitz, Valtencir Zucolotto
Melatonin, the 'chemical signal of darkness', is responsible to regulate biological rhythms and different physiological processes. It is mainly produced by the pineal gland as a hormone in a rhythmic daily basis, but it may also be synthesized by other tissues, such as immune cells, under inflammatory conditions. Its abnormal circulating levels have been related to several diseases such as type 2 diabetes, Alzheimer's disease and some types of cancer. Currently, melatonin is exclusively quantified by ELISA or radioimmunoassays, which although are very sensitive techniques and present low detection limits, usually require specialized personal and equipment, restricting the tests to a limited number of patients...
July 7, 2018: Colloids and Surfaces. B, Biointerfaces
Rijun Gui, Hui Jin, Zonghua Wang, Jinghong Li
Zero-dimensional (0D) black phosphorus quantum dots (BPQDs) are emerging functional nanomaterials. 0D BPQDs are a new form of black phosphorus (BP) nanostructures that were first prepared in 2015; they are different from typical two-dimensional (2D) BP layered nanosheets and one-dimensional (1D) BP nanoribbons. Since 2015, numerous studies have been devoted to exploring various synthetic methods, properties and modifications of BPQDs, which exhibit a broad range of applications. This review systematically summarizes PBQDs for the first time...
July 17, 2018: Chemical Society Reviews
He Wang, Chenxi Zhou, Xiaolu Sun, Yannan Jian, Qingkun Kong, Kang Cui, Shenguang Ge, Jinghua Yu
A novel dual-mode cytosensor based on polyhedral AuPd alloy nanoparticles (PH-AuPd NPs) and three-dimentional reduced graphene oxide (3D-rGO) was constructed for highly sensitive detection of MCF-7 cells. The 3D-rGO was in situ synthesized on the paper working electrode (PWE) by a pollution-free hydrothermal method, increasing the specific surface area and further facilitating the modification of Au nanoparticles (AuNPs). After modified with AuNPs, the Au@ 3D-rGO/PWE was then functionalized by aptamer H1 to trap MCF-7 cells...
July 4, 2018: Biosensors & Bioelectronics
Sunil K Arya, Pedro Estrela
A novel fluidic-based electrochemical ELISA platform is descried for estimation of the bladder cancer protein markers nuclear mitotic apparatus protein 1 (NUMA1) and complement factor H-related 1 (CFHR1). The platform uses an off-site chamber for a sandwich immunoassay and performs the electrochemistry on-chip in a separate chamber. The off-site matrices were connected to the sensor chip in a manner that the sensors were exposed only to the final electroactive product for signal detection, thus avoiding interference from other molecules present in the sample...
July 3, 2018: Biosensors & Bioelectronics
Rezaei Farnaz, Saeidifar Maryam, Javaheri Masoumeh, Sangpour Parvaneh
A novel sustained release carrier of graphene oxide nanosheets functionalized by human serum albumin nanoparticles (FGO-NSs) was synthesized and characterized by field emission scanning electron microscopy (FESEM), zeta sizer analysis, absorption spectroscopy (UV-vis) and Fourier transform infrared spectroscopy (FTIR) studies. The cell viability of GO-NSs, HSA-NPs and FGO-NSs has been tested against human foreskin fibroblast normal cell line, HFFF2 by MTT assay technique. Loading efficiency and release properties of oxaliplatin (OX), as an effective chemotherapeutic agent in the treatment of cancers, from HSA-NPs and FGO-NSs were studied...
July 5, 2018: Colloids and Surfaces. B, Biointerfaces
Damla Kiziltan, Tayfun Vural, Cem Bayram, Serhat Ozturk, Betul Bozdogan, Yesim Tugce Yaman, Serdar Abaci, Emir Baki Denkbas
Early diagnosis of cancer is the most important factor that increases the success of treatment. Therefore, the development of new diagnostic tools is a necessity. In this study, a new electrode surface was developed via modification of a disposable titanium electrode with anodic oxidation and coating of gold nanoparticle and chitosan. Titanium electrodes were anodized by several anodization parameters to obtain a nanoporous surface and characterized by scanning electron microscopy. Electrodes anodized in optimum conditions were modified with gold nanoparticles and chitosan for enhancing conductivity and functionalizing the surface of electrode, respectively...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
Fujian Huang, Meihua Lin, Ruilin Duan, Xiaoding Lou, Fan Xia, Itamar Willner
The spatiotemporal detection of a target mRNA in a single living cell is a major challenge in nanoscience and nanomedicine. We introduce a versatile method to detect mRNA at a single living cell level that uses photocleavable hairpin probes as functional units for the optical (fluorescent) and electrochemical (voltammetric) detection of MnSOD mRNA in single MCF-7 cancer cells. The fluorescent probe is composed of an ortho-nitrophenylphosphate ester functionalized hairpin that includes the FAM fluorophore in a caged configuration quenched by Dabcyl...
July 16, 2018: Nano Letters
Ali Benvidi, Zohreh Abbasi, Marzieh Dehghan Tezerjani, Maryam Banaei, Hamid Reza Zare, Hossein Molahosseini, Shahriar Jahanbani
In this study, a simple and novel electrochemical biosensor based on a glassy carbon electrode (GCE) modified with a composite of graphene oxide (GO) - silk fibroin nanofibers (SF) and gold nanoparticles (MCH/ssDNA/AuNPs/SF/GO/GCE) was developed for detection of DNA sequences. The fabrication processes of electrochemical biosensor were characterized by scanning electron microscopy (SEM), FT-IR and electrochemical methods. Some experimental conditions such as immobilization time of probe DNA and MCH incubation time, time and temperature of hybridization were optimized...
June 2018: Acta Chimica Slovenica
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