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https://www.readbyqxmd.com/read/30539451/microtomy-cutting-formalin-fixed-paraffin-embedded-sections
#1
Joanne Sy, Lee-Cyn Ang
Formalin-fixed, paraffin-embedded tissue (FFPE) still plays an important role in biobanking, since it is comparatively easy to obtain and store in comparison to fresh frozen tissue. They are stored as paraffin or FFPE blocks. Unstained slides derived from FFPE blocks may be used for hematoxylin and eosin histology, special stains, immunohistochemistry, and chromogenic or fluorescent in situ hybridization. In addition, tissue scraped off FFPE slides or from scrolls of FFPE tissue may be used for molecular or proteomic analyses...
2019: Methods in Molecular Biology
https://www.readbyqxmd.com/read/30536837/postoperative-pain-after-glide-path-preparation-using-manual-reciprocating-and-continuous-rotary-instruments-a-randomized-clinical-trial
#2
C Keskin, Ö Sivas Yilmaz, U Inan, Ö Özdemir
AIM: to compare the incidence, intensity and prediction of postoperative pain after glide path preparation with manual, continuous rotary and a novel reciprocating glide path instrument. METHODOLOGY: The study included 240 patients, which were treated by 4 specialists according to a planned treatment protocol. Following access cavity preparation and prior to glide path preparation, the subjects were randomly assigned to 1 of the 3 groups according to the glide path instrument by choosing a sealed envelope containing the group name: R-Pilot (VDW, Munich, Germany), ProGlider (Dentsply Sirona, Ballaigues, Switzerland) and stainless-steel K-files (Dentsply Sirona) (n = 80)...
December 8, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/30536359/effects-of-formaldehyde-on-vegf-mmp2-and-osteonectin-levels-in-periodontal-membrane-and-alveolar-bone-in-the-rats
#3
Nihat Laçin, Bozan Serhat İzol, Mehmet Cudi Tuncer, Ebru Gökalp Özkorkmaz, Buşra Deveci, Engin Deveci
BACKGROUND: The objective of this study was to investigate if long term formaldehyde inhalation may effect periodontal membrane and alveolar bone loss leading to periodontitis. the negative effects of formaldehyde was described using vascular endothelial growth factor (VEGF), matrix metallopeptidase 2 (MMP2) and Osteonectin antibodies involved in the extracellular matrix and angiogenetic development. MATERIALS AND METHODS: Thirty adult Wistar albino rats were used in this study...
December 11, 2018: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/30536212/a-valveless-pulsatile-pump-for-the-treatment-of-heart-failure-with-preserved-ejection-fraction-a-simulation-study
#4
Marcus Granegger, Hitendu Dave, Walter Knirsch, Bente Thamsen, Martin Schweiger, Michael Hübler
PURPOSE: Effective treatment of patients with terminal heart failure and preserved ejection fraction (HFpEF) is an unmet medical need. The aim of this study was to investigate a novel valveless pulsatile pump as a therapeutic option for the HFpEF population through comprehensive in silico investigations. METHODS: The pump was simulated in a numerical model of the cardiovascular system of four HFpEF phenotypes and compared to a typical case of heart failure with reduced ejection fraction (HFrEF)...
December 10, 2018: Cardiovascular Engineering and Technology
https://www.readbyqxmd.com/read/30530953/rheological-parameters-of-saliva-in-comparison-with-taste-examination
#5
Anna Marcinkowska-Gapińska, Kamila Linkowska-Świdzińska, Teodor Świdziński, Anna Surdacka
BACKGROUND: It is generally unknown if taste sensitivity is dependent upon saliva viscosity. The rheological properties of saliva result from many factors and it has been shown to behave as a non-Newtonian fluid whose viscosity decreases with increasing shear rate. Taste sensitivity may be quantitatively assayed by electro-gustometry. OBJECTIVES: The aim of this work was to compare saliva rheological properties, obtained with a rotary-oscillating rheometer, to quantitative measures of taste sensitivity, using electro-gustometry...
2018: Biorheology
https://www.readbyqxmd.com/read/30522276/digital-acoustic-sensor-performance-across-the-infrasound-range-in-non-isolated-conditions
#6
Karina Asmar, Milton Garcés, Darren Hart, Brian Williams
The next generation of acoustic sensors is emerging to supplement legacy sensors traditionally used in regional and global networks. These devices operate under similar principles as traditional sensors, without the need of a separate external digitizer. The calibration of these sensors against their predecessors is crucial to the modernization of conventional technologies. This work describes the characterization of the next-generation MB3 digital microbarometer and the iPrecision smartphone microphone in a non-isolated calibration room across the infrasound (i...
November 2018: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/30521577/patient-reported-outcomes-associated-with-different-restorative-techniques-in-pediatric-dentistry-a-systematic-review-and-mtc-meta-analysis
#7
Nathalia Miranda Ladewig, Tamara Kerber Tedesco, Thaís Gimenez, Mariana Minatel Braga, Daniela Prócida Raggio
BACKGROUND: Despite the increasing number of studies evaluating patient reported outcome measures (PROs), there is no clearness regarding which restorative treatment offers major benefits based on the pediatric patient perspective. AIM: To compare different restorative techniques in pediatric dentistry regarding patient-reported outcomes. DESIGN: Literature searching was carried out on prospective studies indexed in PubMed, Scopus and OpenGrey...
2018: PloS One
https://www.readbyqxmd.com/read/30517444/antibacterial-efficacy-of-synthetic-and-natural-derived-novel-endodontic-irrigant-solutions
#8
Larissa Tais Soligo, Ediléia Lodi, Ana Paula Farina, Matheus Albino Souza, Cristina de Mattos Pimenta Vidal, Doglas Cecchin
The aim of this study was to compare the efficacy of grape seed extract (GSE), calcium hypochlorite [Ca(ClO)2], and sodium hypochlorite (NaOCl) irrigant solutions with rotary or reciprocating instrumentation for disinfection of root canals inoculated with Enterococcus faecalis. The mesiobuccal root canals of mandibular molars were prepared and inoculated with Enterococcus faecalis for 21 days. The roots were then randomly divided into the following eight experimental groups (n=11) according to the instrumentation technique and disinfection protocol: ProTaper Next or Reciproc R25 with sodium chloride (control group), 6% NaOCl, 6% Ca(ClO)2, or 50% GSE used for irrigation during instrumentation...
September 2018: Brazilian Dental Journal
https://www.readbyqxmd.com/read/30515976/reactive-inkjet-printing-of-functional-silk-stirrers-for-enhanced-mixing-and-sensing
#9
Yu Zhang, David A Gregory, Yi Zhang, Patrick J Smith, Stephen J Ebbens, Xiubo Zhao
Stirring small volumes of solution can reduce immunoassay readout time, homogenize cell cultures, and increase enzyme reactivity in bioreactors. However, at present many small scale stirring methods require external actuation, which can be cumbersome. To address this, here, reactive inkjet printing is shown to be able to produce autonomously rotating biocompatible silk-based microstirrers that can enhance fluid mixing. Rotary motion is generated either by release of a surface active agent (small molecular polyethylene glycol) resulting in Marangoni effect, or by catalytically powered bubble propulsion...
December 5, 2018: Small
https://www.readbyqxmd.com/read/30511430/investigation-of-mineral-content-of-root-canal-dentin-after-the-application-of-various-antibiotic-paste-using-energy-dispersive-x-ray-detector
#10
H Simsek, M Coruh, F Cakici, F Fundaoglu Kucukekenci, T Gurbuz, E B Cakici
The purpose of this study was to evaluate mineral content of root canal dentin after treatment with different antibiotic pastes including the mixture of metronidazole, ciprofloxacin, doxycycline, cefaclor, amoxicillin, or minocycline. Fifty extracted maxillary canine teeth were randomly divided into five groups (n = 10 teeth for each group). Root canals were prepared Reciproc rotary files. Canals were irrigated using 5 ml 5% NaOCl and 1 ml 15% EDTA. Each tooth in all groups were longitudinally splitted into two pieces as a control and experimental samples...
December 3, 2018: Microscopy Research and Technique
https://www.readbyqxmd.com/read/30510135/identification-of-two-segments-of-the-%C3%AE-subunit-of-atp-synthase-responsible-for-the-different-affinities-of-the-catalytic-nucleotide-binding-sites
#11
Nelli Mnatsakanyan, Yunxiang Li, Joachim Weber
ATP synthase uses a rotary mechanism to couple transmembrane proton translocation to ATP synthesis and hydrolysis, which occur at the catalytic sites in the β subunits. In presence of Mg2+ , the three catalytic sites of ATP synthase have vastly different affinities for nucleotides, and the position of the central γ subunit determines which site has high, medium, or low affinity. Affinity differences and their changes as rotation progresses underpin the ATP synthase catalytic mechanism. Here, we used a series of variants with up to 45- and 60-residue-long truncations of the N- and C-terminal helices of the γ subunit, respectively, to identify the segment(s) responsible for the affinity differences of the catalytic sites...
December 3, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/30508884/posterior-temporary-c1-2-fixation-for-3-part-fractures-of-the-axis-odontoid-dens-and-hangman-fractures
#12
Suomao Yuan, Bin Wei, Yonghao Tian, Jun Yan, Wanlong Xu, Lianlei Wang, Xinyu Liu
Case series study.To report the clinical outcomes of posterior temporary C1-2 fixation for 3-part fracture of the axis (Type II odontoid fracture according to Grauer classification combined with Hangman fracture).The 3-part fracture of the axis is rare and the treatment is controversy.A total of 8 patients with 3-part fracture of the axis were included in this study. X-rays, CT, and MRI prior to surgery were used to evaluate the cervical spine injury. Grauer classification, fracture angulation, and fracture translation were used to evaluate the fracture of dens...
November 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/30505367/separation-of-nickel-titanium-rotary-and-reciprocating-instruments-a-mini-review-of-clinical-studies
#13
REVIEW
Marcelo Santos Coelho, Marcos de Azevêdo Rios, Carlos Eduardo da Silveira Bueno
Objective: This review aimed to evaluate clinical studies that assessed separated NiTi rotary and reciprocating instruments. Design and Methods: This review assessed clinical studies involving treatments performed by undergraduate students, graduate students, and endodontic specialists. This review evaluated studies using rotary instruments, reciprocating instruments, and hybrid techniques. The number of uses of the different NiTi rotary and reciprocating systems was also assessed...
2018: Open Dentistry Journal
https://www.readbyqxmd.com/read/30505138/miniature-bulk-pzt-traveling-wave-ultrasonic-motors-for-low-speed-high-torque-rotary-actuation
#14
Prakruthi Hareesh, Don L DeVoe
Traveling wave ultrasonic micromotors fabricated from a single layer of homogeneous bulk piezoelectric lead zirconate titanate (PZT) are described. The miniature motors are capable of bi-directional rotary motion with controllable speeds. By taking advantage of transverse interdigitated electrodes to excite traveling waves in a patterned bulk PZT substrate, the monolithic micromotor stators are patterned using a simple and low cost fabrication technique based on micropowder blasting. Performance of the ultrasonic micromotors is explored using devices with integrated glass rotors, using defined preload forces applied between the microfabricated stator and rotor elements...
June 2018: Journal of Microelectromechanical Systems
https://www.readbyqxmd.com/read/30501973/effects-of-pedicle-torsion-on-dynamic-perforasome-survival-in-a-multiterritory-perforator-flap-model-an-experimental-study
#15
Long Wang, Hongqiang Wu, Chen Lin, Zihuai Huang, Renhao Jiang, Xianyao Tao, Jian Ding, Weiyang Gao
BACKGROUND: Necrosis of propeller flaps is a problem in clinical practice. This study was performed to investigate the effects of pedicle torsion on dynamic perforasome survival using a multiterritory perforator flap model in rats. METHODS: Intercostal artery perforator flaps (IC flaps) containing two adjacent dynamic perforasomes were applied to both sides of the dorsum in 15 rats. The IC vessels were dissected carefully under 10 × magnification. A 360° arc of pedicle torsion was applied to the right IC flaps, which comprised the rotary group...
November 2, 2018: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/30501289/performance-analysis-and-experimental-study-of-traveling-wave-type-rotary-ultrasonic-motor-in-high-rotation-environment
#16
Haipeng Chen, Chao Chen, Junshan Wang, Mingyou Shi
In this work, the performance evolution of a traveling wave type rotary ultrasonic motor (TRUM) in a high-rotating environment is studied. The dynamic model of the stator of the TRUM in the centrifugal field is established, and the centrifugal stiffening effect on the stator caused by the centrifugal force is described. Furthermore, the warping deflection of the stator caused by the centrifugal force is analyzed. It is found to change the original contact state and decrease the performance of the TRUM. The contact model between the stator and rotor in the centrifugal field is established...
November 2018: Review of Scientific Instruments
https://www.readbyqxmd.com/read/30501183/3d-printing-the-second-dawn-of-lab-on-valve-fluidic-platforms-for-automatic-bio-chemical-assays
#17
David J Cocovi-Solberg, Maria Rosende, Michal Michalec, Manuel Miró
In this work, inexpensive manufacturing of unibody transparent mesofluidic platforms for pressure-driven Lab-On-a-Valve (LOV) methodologies is accomplished via rapid one-step 3D prototyping from digital models by user-friendly freeware. Multichannel architecture having 800-1800 µm cross-section features with unconventional 3D conduit structures and integrating optical and electrochemical detection facilities is for the first time reported. User-defined flow-programming capitalizing upon software control for automatic liquid handling is synergistically combined with additive manufacturing harnessing stereolithographic 3D printing so as to launch the so-called 4th generation of micro-flow analysis (3D-µFIA)...
December 2, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30501061/characterization-and-antiproliferative-activity-of-a-novel-2-aminothiophene-derivative-%C3%AE-cyclodextrin-binary-system
#18
Elayne Barros Ferreira, Walter Ferreira da Silva Júnior, Jonas Gabriel de Oliveira Pinheiro, Aldilane Gonçalves da Fonseca, Telma Maria Araújo Moura Lemos, Hugo Alexandre de Oliveira Rocha, Eduardo Pereira de Azevedo, Francisco Jaime Bezerra Mendonça Junior, Ádley Antonini Neves de Lima
The novel 2-aminothiophene derivative 2-amino-4,5,6,7-tetrahydrobenzo[ b ]thiophene-3-carbonitrile (6CN) has shown potential anti-proliferative activity in human cancer cell lines. However, the poor aqueous solubility of 6CN impairs its clinical use. This work aimed to develop binary 6CN-β-cyclodextrin (βCD) systems with the purpose of increasing 6CN solubility in water and therefore, to improve its pharmacological activity. The 6CN-βCD binary systems were prepared by physical mixing, kneading and rotary evaporation methods and further characterized by FTIR, XRD, DSC, TG and SEM...
November 29, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30496205/predicting-the-mean-first-passage-time-mfpt-to-reach-any-state-for-a-passive-dynamic-walker-with-steady-state-variability
#19
Isuri Wijesundera, Malka N Halgamuge, Ampalavanapillai Nirmalathas, Thrishantha Nanayakkara
Idealized passive dynamic walkers (PDW) exhibit limit cycle stability at steady state. Yet in reality, uncertainty in ground interaction forces result in variability in limit cycles even for a simple walker known as the Rimless Wheel (RW) on seemingly even slopes. This class of walkers is called metastable walkers in that they usually walk in a stable limit cycle, though guaranteed to eventually fail. Thus, control action is only needed if a failure state (i.e. RW stopping down the ramp) is imminent. Therefore, efficiency of estimating the time to reach a failure state is key to develop a minimal intervention controller to inject just enough energy to overcome a failure state when required...
2018: PloS One
https://www.readbyqxmd.com/read/30487106/pneumatic-rock-drill-vs-electric-rotary-hammer-drill-productivity-vibration-dust-and-noise-when-drilling-into-concrete
#20
David Rempel, Andrea Antonucci, Alan Barr, Michael R Cooper, Bernard Martin, Richard L Neitzel
OBJECTIVES: Both pneumatic rock drills and electric rotary hammer drills are used for drilling large holes (e.g., 10-20 mm diameter) into concrete for structural upgrades to buildings, highways, bridges, and airport tarmacs. However, little is known about the differences in productivity, and exposures to noise, handle vibration, and dust between the two types of drills. The aim of this study was to compare these outcomes with similar mass electric rotary and pneumatic rock drills drilling into concrete block on a test bench system...
January 2019: Applied Ergonomics
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