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Jaroslaw Pieróg, Kleanthis Fytianos, Luca Tamò, Cedric Simillion, Adriano Taddeo, Gregor Kocher, Mathias Gugger, Tomasz Grodzki, Manfred Heller, Fabian Blank, Thomas Geiser, Ralph A Schmid, Amiq Gazdhar
OBJECTIVES: Stem cells secrete significant amounts of bioactive factors in their secretome that can be immunosuppressive. We studied the effect of the secretome obtained from bone marrow-derived mesenchymal stem cells (BMSC-sec) in combination with cyclosporine A following acute rejection of lung allografts in the rat. METHODS: Lung allotransplants were performed from male Brown Norway donor rats to recipient male Fisher 344 rats. Rat BMSC-sec was introduced intratracheally in the recipient every day after the transplant until the day the animal was sacrificed...
December 1, 2018: Interactive Cardiovascular and Thoracic Surgery
L Bouard, C Bodet-Milin, C Bailly, T Guillaume, P Peterlin, A Garnier, A Le Bourgeois, B Mahé, V Dubruille, N Blin, C Touzeau, T Gastinne, A Lok, A Bonnet, Marie C Béné, S Le Gouill, P Moreau, F Kraeber-Bodéré, P Chevallier
The impact of early Fluorine-18 Fluorodeoxyglucose positron emission tomography/computed tomography (FDG PET-CT) status on survival after allogeneic transplantation for lymphoma is poorly reported.This retrospective study included all adult Hodgkin (HL) or non-Hodgkin (NHL) lymphoma patients (>18 years old) who benefited from FDG PET-CT before (within 1 month) and/or early (+3 months and within +6-9 months) after Allo-SCT in our institution between 2005 and 2015 and who were still without documented progression nor relapse at the time of the FDG PET-CT...
November 24, 2018: Biology of Blood and Marrow Transplantation
Ying-Jun Chang, Lan-Ping Xu, Yu Wang, Xiao-Hui Zhang, Huan Chen, Yu-Hong Chen, Feng-Rong Wang, Wei Han, Yu-Qian Sun, Chen-Han Yan, Fei-Fei Tang, Xiao-Dong Mo, Kai-Yan Liu, Xiao-Jun Huang
This long-term follow-up study evaluated the effects of corticosteroid prophylaxis on GVHD-free, relapse-free survival (GRFS) based on a controlled open-label randomized trial in which 228 allotransplant recipients were categorized as low-risk (N = 83, group A) or high-risk; patients at high-risk were randomly assigned to receive (N = 72, group B) or not receive (N = 73, group C) low-dose methylprednisolone prophylaxis. The cumulative incidences of chronic GVHD, relapse, NRM, LFS, OS, and GRFS were 60%, 19%, 16%, 68%, 73%, and 46%, respectively, in the total cases...
November 24, 2018: Biology of Blood and Marrow Transplantation
Abhijit Jagdale, Hayato Iwase, Edwin Klein, David K C Cooper
There is an increased incidence of certain tumors and other neoplastic disease in organ allotransplant recipients receiving immunosuppressive therapy. Following clinical pig organ xenotransplantation, will there be a risk of the development of neoplasia in the pig graft or in other tissues transplanted with it, eg, lymph nodes? The incidence of neoplasia in young slaughterhouse pigs is very low (<0.005%), but in older pigs is largely unknown (as most pigs are killed within the first six months of life). However, lymphosarcoma, nephroblastoma, and melanoma have been reported in pigs...
November 9, 2018: Xenotransplantation
I S Mukhamadeev, K V Prokhorov, A A Oborin
We present a clinical case of successful correction of aneurismatic dilatation of the anastomosis zone after allotransplant implantation. In 2004 this patient underwent implantation of an arterial allotransplant of "complex architectonics" because of trauma of the iliofemoral arterial segment. After 12 years he reported appearance of discomfort in the left side of the abdomen. Duplex ultrasound scanning revealed aneurism at the site of anastomosis of the prosthesis with internal iliac artery. Therefore, we performed endovascular isolation of the aneurism with the use of a stent-graft and restoration of blood flow down to the extremity periphery has been performed...
June 2018: Kardiologiia
Lauren Veltri, Katayoun Rezvani, Betul Oran, Rohtesh Mehta, Gabriela Rondon, Partow Kebriaei, Uday Popat, Yago Nieto, Chitra Hosing, Muzaffar Qazilbash, Issa Khouri, Elizabeth Shpall, Richard Champlin, David Marin
The outcome of persons > 65 years with acute myeloid leukemia (AML) is poor. A transplant from an HLA-identical sibling or an HLA-matched unrelated donor can cure some of these patients but is associated with a substantial transplant-related mortality and a high relapse risk. We analyzed 185 subjects > 65 years with high-risk AML receiving conventional (n = 42) or reduced-intensity (n = 143) pretransplant conditioning and a transplant from an HLA-identical sibling (n = 66) or a 10/10 loci HLA-matched unrelated donor (n = 119)...
October 9, 2018: Biology of Blood and Marrow Transplantation
Shaun D Mendenhall, Michael T Ginnetti, Justin D Sawyer, Steven J Verhulst, Bradford L West, L Scott Levin, Michael W Neumeister
Background: Vascularized composite allotransplantation (VCA) is an emerging and growing field. Little is known about the prevalence and distribution of the adult potential donor population in the United States now that it falls under the oversight of the United Network for Organ Sharing (UNOS). Methods: We assessed the UNOS database from 2008 to 2015 to estimate the prevalence and distribution of adult potential vascularized composite allograft donors. Donor inclusion and exclusion criteria were developed in a way to minimize risk to recipients and were applied to the dataset...
June 2018: Plastic and Reconstructive Surgery. Global Open
Anoma Nellore, Jay A Fishman
Post-transplantation infections are common in allograft recipients and should be expected in all immunocompromised hosts. Based on the need for immunosuppression in xenotransplantation, procedures developed to enhance safety in allotransplantation can be applied in future xenotransplantation clinical trials. Standardized approaches can be developed to guide the evaluation of common infectious syndromes in xenograft recipients. The opportunity created by screening of swine intended as xenograft donors has equal applicability to allotransplantation-notably broader screening strategies for allograft donors such as use of advanced sequencing modalities including broad-range molecular probes, microarrays, and high-throughput pyrosequencing...
July 2018: Xenotransplantation
Sara Salehi, Kenny Tran, Warren L Grayson
In the past, a diagnosis of organ failure would essentially be a death sentence for patients. With improved techniques for organ procurement and surgical procedures, transplantations to treat organ failure have become standard medical practice. However, while the demand for organs has skyrocketed, the donor pool has not kept pace leading to long recipient waiting lists. Organ preservation provides a means to increase the number of available transplantable organs. However, there are significant drawbacks associated with cold storage, the current gold standard...
September 2018: Yale Journal of Biology and Medicine
Maximilian Christopeit, Myriam Labopin, Norbert-Claude Gorin, Francesco Saraceni, Jakob Passweg, Edouard Forcade, Johan Maertens, Maria Teresa Van Lint, Alberto Bosi, Dietger Niederwieser, Gerhard Ehninger, Emmanuelle Polge, Mohamad Mohty, Arnon Nagler
Patients with acute myeloid leukemia (AML) who relapse after autologous stem cell transplantation (ASCT) can be rescued by allogeneic SCT. We identified 537 adult patients with AML allografted in second complete remission (CR2) or first relapse after ASCT in the European Society for Blood and Marrow Transplantation (EBMT) registry. At 3 years post allograft, leukemia free survival (LFS) was 31.4% [95%CI 27.3-35.6], overall survival (OS) 39.5% [95%CI 35.1-43.9], relapse incidence (RI) 34.6% [95%CI 30.4-38.8], and nonrelapse mortality (NRM) 33...
September 14, 2018: American Journal of Hematology
Negar Karimaghai, Amin Tamadon, Farhad Rahmanifar, Davood Mehrabani, Alireza Raayat Jahromi, Shahrokh Zare, Zahra Khodabandeh, Iman Razeghian Jahromi, Omid Koohi-Hoseinabadi, Mehdi Dianatpour
Objectives: Adipose tissue-derived mesenchymal stem cells (AT-MSCs) with more potent immunomodulatory effects, greater proliferative potential and secretion of growth factors and cytokines in comparison with bone marrow derived MSCs are more appropriate for cell therapy. The aims of the present study were to evaluate the histomorphometric effect of AT-MSCs allotransplantation on regeneration of germinal layer cells of seminiferous tubules in busulfan-induced azoospermic hamsters. Materials and Methods: In the present experimental case-control study, AT-MSCs were isolated from adipose tissue of two female and six male donor albino hamsters, and testes of the males were simultaneously used as negative control group...
July 2018: Iranian Journal of Basic Medical Sciences
Bin Pan, Fan Xia, Yujing Wu, Fan Zhang, Zhenzhen Lu, Ruixue Fu, Longmei Shang, Lingling Li, Zengtian Sun, Lingyu Zeng, Kailin Xu
Acute graft-versus-host disease (aGVHD) remains a major challenging complication of patients receiving allogeneic hematopoietic cell transplantation (allo-HCT). CD4+ effector T cells and their related cytokines mediate pathogenesis of aGVHD, in which donor-T-cell derived interleukin-22 (IL-22) was recently indicated to play a role. The role of recipient-derived IL-22 in aGVHD remains to be elucidated. By applying IL-22 knock out (IL-22KO) mice as recipients of allotransplant, we found recipient derived IL-22 alleviated aGVHD and improved survival of allotransplant recipients...
August 13, 2018: Cytokine
Charles Anton Fries, Carole Yasay Villamaria, Jerry Richard Spencer, Shari Lawson, Lin Wang, Thomas Raj, E George Wolf, Bijaya Kumar Parida, Vijay Saradhi Gorantla, Rory Frederick Rickard, Michael Robert Davis
BACKGROUND:  Ischemia-reperfusion injury (IRI) precipitates acute rejection of vascularized composite allografts (VCA). Hyperbaric preservation of tissues ex vivo, between harvest and revascularization, may reduce IRI and mitigate acute rejection of VCA. METHODS:  A porcine heterotopic musculocutaneous gracilis flap model was used. In phase 1, control autografts ( n  = 5) were infused with University of Wisconsin Solution (UWS) and stored at 4°C for 3 hours...
August 12, 2018: Journal of Reconstructive Microsurgery
Thomas Kerforne, Geraldine Allain, Sebastien Giraud, Delphine Bon, Virginie Ameteau, Pierre Couturier, William Hebrard, Jerome Danion, Jean-Michel Goujon, Raphael Thuillier, Thierry Hauet, Benoit Barrou, Christophe Jayle
Kidneys from donation after circulatory death (DCD) are highly sensitive to ischemia-reperfusion injury and thus require careful reconditioning, such as Normothermic Regional Perfusion (NRP). However, the optimal NRP protocol remains to be characterized. NRP was modeled in a DCD porcine model (30min of cardiac arrest) for 2h, 4h or 6h compared to a control group (noNRP); kidneys were machine-preserved and allotransplanted. NRP appeared to permit recovery from warm ischemia, possibly due to increased expression of HIF1α-dependent survival pathway...
August 9, 2018: American Journal of Transplantation
D Szubińska-Lelonkiewicz, M Osiak, P Wychowański, M Siewert-Gutowska, P Fiedor
Recipients of allotransplants are more susceptible to viral infections, among which the human papilloma virus infection is an independent factor inducing precancerous lesions and cancers of both the anogenital and the cervicocephalic region. MATERIALS AND METHODS: The study included a group of 69 allogenic kidney transplantation recipients aged 20 to 70, who were treated with cyclosporine, azathioprine, and prednisone. The patients in whom the macroscopic examination of the oral mucosa revealed lesions were qualified for a biopsy...
July 2018: Transplantation Proceedings
Yu-Chi Wang, Rong-Fu Chen, Gerald Brandacher, W P Andrew Lee, Yur-Ren Kuo
BACKGROUND: Our previous studies demonstrated that adipose-derived stem cells (ASCs) could modulate regulatory T cells (Treg) and prolong hind-limb allotransplant survival in vitro and in vivo. Dendritic cells (DCs) play a pivotal role in innate and adaptive immunity. The aim of this study is to investigate the underlying mechanism of ASCs in modulating DC maturation. MATERIALS AND METHODS: ASCs were isolated from rodent adipose tissue, DCs were derived from the bone marrow, and CD4+ T cells were purified from splenocytes...
September 15, 2018: Experimental Cell Research
John S Riley, Lauren E McClain, John D Stratigis, Barbara E Coons, Haiying Li, Heather A Hartman, William H Peranteau
In utero hematopoietic cell transplantation (IUHCT) is a nonmyeloablative nonimmunosuppressive alternative to postnatal hematopoietic stem cell transplantation for the treatment of congenital hemoglobinopathies. Anti-HLA donor-specific Abs (DSA) are associated with a high incidence of graft rejection following postnatal hematopoietic stem cell transplantation. We determine if DSA present in the mother can similarly cause graft rejection in the fetus following IUHCT. Ten million C57BL/6 (B6, H2kb ) bone marrow cells were transplanted in utero into gestational day 14 BALB/c (H2kd ) fetuses...
September 1, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Yue Zhao, Yu Jia, Lu Wang, Song Chen, Xia Huang, Bingyang Xu, Guangyuan Zhao, Ying Xiang, Jun Yang, Gang Chen
Heme oxygenase-1 (HO-1) is critical for the ability of immature dendritic cells (imDCs) to suppress T-cell responses. Induction of high HO-1 expression may markedly improve the tolerogenic capacity of imDCs. Here, we generated bone marrow-derived DCs (BMDCs) from BALB/c mice with low doses of GM-CSF and IL-4. The adherent BMDCs were obtained as imDCs. Upregulation of HO-1 in imDCs (HO-1hi -imDCs) was achieved by cobalt protoporphyrin treatment. HO-1hi -imDCs proved to be more maturation-resistant than conventional imDCs, with an enhanced ability to inhibit allogeneic T-cell proliferation stimulated by anti-CD3/CD28 antibodies...
2018: Frontiers in Immunology
Xie-Na Cao, Yuan Kong, Yang Song, Min-Min Shi, Hong-Yan Zhao, Qi Wen, Zhong-Shi Lyu, Cai-Wen Duan, Yu Wang, Lan-Ping Xu, Xiao-Hui Zhang, Xiao-Jun Huang
Graft-versus-host disease (GVHD) is a major complication after allogeneic haematopoietic stem cell transplantation (allo-HSCT) that is frequently associated with bone marrow (BM) suppression, and clinical management is challenging. BM endothelial progenitor cells (EPCs) play crucial roles in the regulation of haematopoiesis and thrombopoiesis. However, little is known regarding the functional roles of BM EPCs in acute GVHD (aGVHD) patients. In the current prospective case-control study, reduced and dysfunctional BM EPCs, characterized by decreased migration and angiogenesis capacities and increased levels of reactive oxygen species (ROS) and apoptosis, were found in aGVHD patients compared with those without aGVHD...
September 2018: British Journal of Haematology
Krishna S Vyas, Anita T Mohan, Shane D Morrison, Duy C Tran, Samir Mardini
BACKGROUND:  Dendritic cells (DCs) are bone marrow-derived, professional antigen-presenting cells with tolerogenic function. The ability of DCs to regulate alloantigen-specific T cell responses and to promote tolerance has aligned them ideally for a role in vascularized composite allotransplantation (VCA). In this study, we summarize the current evidence for DC therapies for tolerance induction to alleviate the requirement for chronic immunosuppression. METHOD:  A comprehensive and structured review of manuscripts published on VCA was performed using the MEDLINE and PubMed databases...
October 2018: Journal of Reconstructive Microsurgery
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