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skin senescence

Qiming Wu, Shuang E, Kazushi Yamamoto, Tsuyoshi Tsuduki
This study used senescence-accelerated prone mice (SAMP8) to examine the effects of a carbohydrate-restricted diet on aging and skin senescence, to determine how long-term carbohydrate restriction affects the aging process. Three-week-old male SAMP8 mice were divided into three groups after 1 week of preliminary feeding: one was given a controlled diet, the other was given a high-fat diet, and the third was given a carbohydrate-restricted diet. Ad libitum feeding was administered until the mice reached 50 weeks of age...
October 3, 2018: Biogerontology
Jung Eun Yoon, Yeongeun Kim, Soohyun Kwon, Misun Kim, Young Hwa Kim, Jang-Hee Kim, Tae Jun Park, Hee Young Kang
Cutaneous ageing is an important extrinsic process that modifies the pigmentary system. Because cellular senescence is a fundamental ageing mechanism, we examined the role of senescent cells in ageing pigmentation. Methods: Biopsies obtained from senile lentigo and perilesional normal skin were assayed for a marker of cellular senescence, p16INK4A . To determine the secretory phenotypes of senescent fibroblasts, we performed microarray, RNA sequencing and methylation array analyses in senile lentigo and senescent fibroblasts...
2018: Theranostics
Rui Wang, Yupeng He, Valerie Robinson, Ziping Yang, Paul Hessler, Loren M Lasko, Xin Lu, Anahita Bhathena, Albert Lai, Tamar Uziel, Lloyd T Lam
Metastatic melanoma is responsible for approximately 80 percent of deaths from skin cancer. Microphthalmia-associated transcription factor (MITF) is a melanocyte-specific transcription factor that plays an important role in the differentiation, proliferation and survival of melanocytes as well as in melanoma oncogenesis. MITF is amplified in approximately 15% of metastatic melanoma patients. However, no small molecule inhibitors of MITF currently exist. MITF was shown to associate with p300/CBP, members of the KAT3 family of histone acetyltransferase (HAT)...
September 28, 2018: Molecular Cancer Therapeutics
Hyun-Min Kim, Jin Hyuk Jung, Ji Yea Kim, Jina Heo, Dae-Hyun Cho, Hee-Sik Kim, Sungkwan An, In-Sook An, Seunghee Bae
Skin photoaging, which is mainly induced by ultraviolet B (UVB) radiation, is prevented by the application of UV-protective agents. The microalga Nannochloropsis oceanica (N. oceanica) has been primarily reported as a potential biofuel; however, in this study, we investigated whether N. oceanica extracts exerted photoprotective effects against UVB-irradiated human dermal fibroblasts (HDFs) and which single component was responsible for the protective effect of the extracts. Two extracts-pigment and non-pigment-were prepared from N...
September 28, 2018: Photochemistry and Photobiology
Jae-Hong Jeong, Mi-Bo Kim, Changhee Kim, Jae-Kwan Hwang
Vitamin C significantly reduced senescence-associated β-galactosidase (SA-β-gal) activity, with both the suppression of cell-cycle inhibitors (p53, p21, p16, and pRb) and stimulation of cell-cycle activators (E2F1 and E2F2). Vitamin C also effectively attenuated the hyperactivation of the phosphatidylinositol 3-kinase (PI3K)/protein kinase-B (AKT) signaling pathway. The expression of the longevity marker, the mammalian target of rapamycin (mTOR), was down-regulated by vitamin C while the expressions of forkhead box O3a (FoxO3a) and sirtuin1 (SIRT1) were up-regulated by vitamin C...
April 2018: Food Science and Biotechnology
Yun-Ui Bae, Youlim Son, Chang-Hyun Kim, Kwang Seok Kim, Se Hee Hyun, Hyun Goo Woo, Byul A Jee, Jun-Hyuk Choi, Hoon-Ki Sung, Hyung-Chul Choi, So Young Park, Ju-Hyun Bae, Kyung-Oh Doh, Jae-Ryong Kim
Senescent cells accumulate in various tissues over time, and contribute to tissue dysfunction and aging-associated phenotypes. Accumulating evidence suggests that cellular senescence can be inhibited through pharmacological intervention, as well as through treatment with soluble factors derived from embryonic stem cells (ESCs). In an attempt to investigate the anti-senescence factors secreted by ESCs, we analyzed mouse ESC-derived extracellular miRNAs in conditioned medium (CM) via miRNA array analysis. We selected mmu-miR-291a-3p as a putative anti-senescence factor via bioinformatics analysis...
September 15, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
Katsutoshi Miura, Kanna Yamashita
Scanning acoustic microscopy (SAM) can assess tissue stiffness by calculating the speed of sound (SOS) through tissues. SOS increases as tissue stiffness increases. Sensitivity to protease digestion depends on protein type, concentration, and modification. We analyzed the SOS images of formalin-fixed paraffin-embedded skin sections from elderly, young, diabetic, and nondiabetic subjects, as well as chronic and acute wounds. SAM provided high-resolution histology similar to LM and revealed characteristic SOS alteration following pepsin treatment...
2018: Pathobiology of Aging & Age related Diseases
Chun-Feng Liu, Xiao-Lin Li, Zi-Long Zhang, Lu Qiu, Shi-Xuan Ding, Jun-Xin Xue, Guo-Ping Zhao, Jian Li
Although the health benefits attributed to urolithin A, such as anti-cancer, anti-inflammatory, and antioxidant effects, are based on numerous, diverse studies carried out in vitro, the biological effects of urolith A are still not entirely understood. In this study, we explored the biological effects of urolithin A using senescent human skin fibroblasts to determine whether urolithin A has any anti-aging potential. Our results showed that urolithin A significantly increased type I collagen expression, and reduced matrix metalloproteinase-1(MMP-1) expression...
September 14, 2018: Rejuvenation Research
Audrey S Wang, Oliver Dreesen
Cellular senescence is an irreversible growth arrest that occurs as a result of different damaging stimuli, including DNA damage, telomere shortening and dysfunction or oncogenic stress. Senescent cells exert a pleotropic effect on development, tissue aging and regeneration, inflammation, wound healing and tumor suppression. Strategies to remove senescent cells from aging tissues or preneoplastic lesions can delay tissue dysfunction and lead to increased healthspan. However, a significant hurdle in the aging field has been the identification of a universal biomarker that facilitates the unequivocal detection and quantification of senescent cell types in vitro and in vivo ...
2018: Frontiers in Genetics
Yong Zhang, Yinhui Dong, Michael W Melkus, Shutao Yin, Su-Ni Tang, Peixin Jiang, Kartick Pramanik, Wei Wu, Sangyub Kim, Min Ye, Hongbo Hu, Junxuan Lu, Cheng Jiang
Bufalin is a major cardiotonic compound in the traditional Chinese medicine, Chansu, prepared from toad skin secretions. Cell culture studies have suggested an anticancer potential involving multiple cellular processes, including differentiation, apoptosis, senescence, and angiogenesis. In prostate cancer cell models, P53-dependent and independent caspase-mediated apoptosis and androgen receptor (AR) antagonism have been described for bufalin at micromolar concentrations. Because a human pharmacokinetic study indicated that single nanomolar bufalin was safely achievable in the peripheral circulation, we evaluated its cellular activity within range with the AR-positive and P53 wild-type human LNCaP prostate cancer cells in vitro Our data show that bufalin induced caspase-mediated apoptosis at 20 nmol/L or higher concentration with concomitant suppression of AR protein and its best-known target, PSA and steroid receptor coactivator 1 and 3 (SRC-1, SRC-3)...
August 30, 2018: Molecular Cancer Therapeutics
Elena M Malinovskaya, Elizaveta S Ershova, Vera E Golimbet, Lev N Porokhovnik, Nataliya A Lyapunova, Serguey I Kutsev, Natalia N Veiko, Svetlana V Kostyuk
Introduction: The multi-copied genes coding for the human 18, 5.8, and 28S ribosomal RNA (rRNA) are located in five pairs of acrocentric chromosomes forming so-called rDNA. Human genome contains unmethylated, slightly methylated, and hypermethylated copies of rDNA. The major research question: What is the rDNA copy number (rDNA CN) and the content of hypermethylated rDNA as a function of age? Materials and Methods: We determined the rDNA CN in the blood leukocyte genomes of 651 subjects aged 17 to 91 years...
2018: Frontiers in Genetics
Seong-Wook Seo, Seul-Ki Park, Soo-Jin Oh, Ok Sarah Shin
Exposure to ultraviolet (UV) radiation is a major contributing factor to premature aging (photoaging) and skin cancer. In vitro models of cellular senescence have proven to be very useful for the study of slow and progressive accumulation of damage resulting in the growth arrest of aging skin cells. In this study, we compared UVA-induced cellular responses in non-senescent (NS) vs. senescent (S) human dermal fibroblasts (HDFs). HDFs were irradiated with a single dose of UVA (7.5 J/cm2) and QuantSeq 3' mRNA sequencing was performed to assess differential gene expression...
2018: PloS One
Elisabetta Mattioli, Davide Andrenacci, Cecilia Garofalo, Sabino Prencipe, Katia Scotlandi, Daniel Remondini, Davide Gentilini, Anna Maria Di Blasio, Sergio Valente, Emanuela Scarano, Lucia Cicchilitti, Giulia Piaggio, Antonello Mai, Giovanna Lattanzi
Defects in stress response are main determinants of cellular senescence and organism aging. In fibroblasts from patients affected by Hutchinson-Gilford progeria, a severe LMNA-linked syndrome associated with bone resorption, cardiovascular disorders, and premature aging, we found altered modulation of CDKN1A, encoding p21, upon oxidative stress induction, and accumulation of senescence markers during stress recovery. In this context, we unraveled a dynamic interaction of lamin A/C with HDAC2, an histone deacetylase that regulates CDKN1A expression...
October 2018: Aging Cell
Valentina Nanni, Lorena Canuti, Angelo Gismondi, Antonella Canini
BACKGROUND: Ultraviolet light exposure generates, in human tissues, radical species, which represent the main cause of photo-aging, DNA damage and skin cancer onset. On the other hand, Mediterranean plants, being continuously subjected to high solar radiation levels, are naturally adapted to take on this type of abiotic stress, thanks to the production of antioxidant secondary metabolites. For these reasons, several plant extracts were documented to be excellent antineoplastic drugs. PURPOSE: We investigated the potential antitumor activity of the flower extract obtained by Spartium junceum L...
July 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Barbara Bellei, Emilia Migliano, Marinella Tedesco, Silvia Caputo, Federica Papaccio, Gianluca Lopez, Mauro Picardo
BACKGROUND: Adipose tissue-derived stem cells are considered to be a promising source in the field of cell therapy and regenerative medicine. In addition to direct cell replacement using adipose tissue or purified stem cells, intercellular molecule exchange by the adipose tissue complex, a vast array of bioactive secretory factors, demonstrated beneficial effects by reducing tissue damage and stimulation of endogenous repair. However, for therapeutic purposes, the use of secretome derivatives, such as full conditioned media or purified exosomes generated in vitro, may present considerable disadvantages for cell manufacturing, storage, product safety, and their potential as a ready-to-go therapeutic product...
August 9, 2018: Stem Cell Research & Therapy
Carlos Pascual-Caro, Maria Berrocal, Aida M Lopez-Guerrero, Alberto Alvarez-Barrientos, Eulalia Pozo-Guisado, Carlos Gutierrez-Merino, Ana M Mata, Francisco Javier Martin-Romero
STIM1 is an endoplasmic reticulum protein with a role in Ca2+ mobilization and signaling. As a sensor of intraluminal Ca2+ levels, STIM1 modulates plasma membrane Ca2+ channels to regulate Ca2+ entry. In neuroblastoma SH-SY5Y cells and in familial Alzheimer's disease patient skin fibroblasts, STIM1 is cleaved at the transmembrane domain by the presenilin-1-associated γ-secretase, leading to dysregulation of Ca2+ homeostasis. In this report, we investigated expression levels of STIM1 in brain tissues (medium frontal gyrus) of pathologically confirmed Alzheimer's disease patients, and observed that STIM1 protein expression level decreased with the progression of neurodegeneration...
October 2018: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
A Di Pietro, A Facciolà, G Visalli
The Herpes Zoster vaccine is strongly recommended by the World Health Organization to promote healthy aging by preventing the corresponding age- related disease, also named shingles. The disease is due to the endogenous reactivation of Varicella Zoster Virus, the causal agent of chickenpox, that becomes latent at the peripheral nervous system level. Here, owing to the host's cell-mediated immunity, it may be confined for several decades. However, the immune senescence allows the possibility of virus reactivation, causing the onset of neuropathic pain and skin rash that characterize the acute disease...
July 2018: Annali di Igiene: Medicina Preventiva e di Comunità
Alexandra Moreno-García, Alejandra Kun, Olga Calero, Miguel Medina, Miguel Calero
Despite aging being by far the greatest risk factor for highly prevalent neurodegenerative disorders, the molecular underpinnings of age-related brain changes are still not well understood, particularly the transition from normal healthy brain aging to neuropathological aging. Aging is an extremely complex, multifactorial process involving the simultaneous interplay of several processes operating at many levels of the functional organization. The buildup of potentially toxic protein aggregates and their spreading through various brain regions has been identified as a major contributor to these pathologies...
2018: Frontiers in Neuroscience
Nguyen T K Vo, Levi C Moore, Katelin W Spiteri, Robert Hanner, Michael P Wilkie, Stephanie J DeWitte-Orr
Lampricides are currently being applied to streams and rivers to control the population of sea lamprey, an invasive species, in the Great Lakes. The most commonly used lampricide agent used in the field is 3-trifluoromethyl-4-nitrophenol (TFM), which targets larval sea lamprey in lamprey-infested rivers and streams. The specificity of TFM is due to the relative inability of sea lamprey to detoxify the agent relative to non-target fishes. There is increasing concern, however, about non-target effects on fishes, particularly threatened populations of juvenile lake sturgeon (LS; Acipenser fulvescens)...
October 30, 2018: Ecotoxicology and Environmental Safety
Paul Hiebert, Mateusz S Wietecha, Michael Cangkrama, Eric Haertel, Eleni Mavrogonatou, Michael Stumpe, Heiko Steenbock, Serena Grossi, Hans-Dietmar Beer, Peter Angel, Jürgen Brinckmann, Dimitris Kletsas, Jörn Dengjel, Sabine Werner
Nrf2 is a key regulator of the antioxidant defense system, and pharmacological Nrf2 activation is a promising strategy for cancer prevention and promotion of tissue repair. Here we show, however, that activation of Nrf2 in fibroblasts induces cellular senescence. Using a combination of transcriptomics, matrix proteomics, chromatin immunoprecipitation and bioinformatics we demonstrate that fibroblasts with activated Nrf2 deposit a senescence-promoting matrix, with plasminogen activator inhibitor-1 being a key inducer of the senescence program...
July 16, 2018: Developmental Cell
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