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vibrio harveyi

Nurliyana Mohamad, Fauzul Aidil Mohd Roseli, Mohammad Noor Amal Azmai, Mohd Zamri Saad, Ina Salwany Md Yasin, Nor Amalina Zulkiply, Nurrul Shaqinah Nasruddin
In September 2016, a marine fish farm operator in Selangor, Malaysia reported a disease outbreak affecting juvenile Hybrid Groupers (Camouflage Grouper Epinephelus polyphekadion x Tiger Grouper E. fuscoguttatus). The average daily mortality was 120 fish, resulting in a cumulative mortality rate of 29% within 10 days. The affected Hybrid Groupers displayed lethargy, excessive mucus production, rotten fins, congestion of livers and kidneys, and enlargement of spleens. Microscopically, there were generalize congestion of the brains and internal organs...
December 7, 2018: Journal of Aquatic Animal Health
Mingkang Chen, Xia Liu, Jing Zhou, Xu Wang, Ruiting Liu, Hongyu Peng, Boyan Li, Zhonglu Cai, Chen Jiang
Galectins are a family of proteins with conserved carbohydrate recognition domains (CRDs) that bind to specific glycans, including the glycans on the surface of pathogens, and therefore play a role in cytokine secretion, cell activation, migration, adhesion and apoptosis. Currently, galectins have been extensively studied in mammalian species but rarely studied in teleost fish species. In this study, a total of 12 galectin genes were characterized to understand the molecular mechanisms of galectin function in Japanese pufferfish (Takifugu rubripes)...
November 27, 2018: Fish & Shellfish Immunology
An Ngoc Nguyen, Elena Disconzi, Guillaume M Charrière, Delphine Destoumieux-Garzón, Philippe Bouloc, Frédérique Le Roux, Annick Jacq
CsrBs are bacterial highly conserved and multiple-copy noncoding small RNAs (sRNAs) that play major roles in cell physiology and virulence. In the Vibrio genus, they are known to be regulated by the two-component system VarS/VarA. They modulate the well-characterized quorum sensing pathway controlling virulence and luminescence in Vibrio cholerae and Vibrio harveyi , respectively. Remarkably, Vibrio tasmaniensis LGP32, an oyster pathogen that belongs to the Splendidus clade, was found to have four copies of csrB , named csrB1-4 , compared to two to three copies in other Vibrio species...
November 28, 2018: MSphere
Zhiming Zhu, Chuanfu Dong, Shaoping Weng, Jianguo He
Vibrio harveyi is a serious pathogen of scale drop and muscle necrosis disease in marine commercial fishes. Adhesion to and colonization of the host cells surfaces is the first and crucial step for pathogenic bacterial infection, which is usually mediated by outer membrane proteins (Omps). The objectives of this study were to identify the major adhesin in Omps that plays the essential role in adhesion of V. harveyi to the host cells, and to assess the potential of this adhesin as a vaccine candidate for V. harveyi infection...
November 16, 2018: Fish & Shellfish Immunology
Yongcan Zhou, Yang Lei, Zhenjie Cao, Xiaojuan Chen, Yun Sun, Yue Xu, Weiliang Guo, Shifeng Wang, Chunsheng Liu
Defensins are a group of small cationic and cysteine-rich peptides that are important components of the innate immune system. However, studies on defensins in teleosts are very limited, particularly studies on defensin functions through in vivo assays. In this study, we cloned and identified one β-defensin (TroBD) the golden pompano, Trachinotus ovatus, and analyzed the functions of TroBD in both in vivo and in vitro assays. TroBD is composed of 63 amino acids and shares high sequence identities (27.27-98...
November 15, 2018: Developmental and Comparative Immunology
Jing Diao, Hongjun Liu, Fawen Hu, Le Li, Xiaolu Wang, Chunlei Gai, Xiaoqing Yu, Ying Fan, La Xu, Haibin Ye
Fat greenling (Hexagrammos otakii) is an important aquaculture fish species in northern China. Unfortunately, Vibrio infections have caused considerable losses to the fat greenling aquaculture industry. However, the study on immune response of fat greenling against Vibrio species has not been reported yet. In this paper, the immune response of fat greenling against V. harveyi at gene expression level was studied by transcriptome analysis. A total of 189753 high-quality unigenes with a N50 length of 672bp were obtained by transcriptome profiling, which provided abundant data for the future study of fat greenling...
October 25, 2018: Fish & Shellfish Immunology
Biao Han, Vaneet Inder Kaur, Kartik Baruah, Viet Dung Nguyen, Peter Bossier
Ascorbate is an essential nutrient commonly regarded as an antioxidant. In this study, using axenic brine shrimp and pathogenic strain Vibrio harveyi as the host-pathogen model, we confirmed that pretreatment of sodium ascorbate (NaAs), at an optimum concentration, was a prooxidant by generation of hydrogen peroxide, inducing protective effects in the brine shrimp against V. harveyi infection. Such a protective effect could be neutralized by the addition of an antioxidant enzyme catalase. We further showed that generation of oxygen radicals is linked to the induction of heat shock protein 70 (Hsp70), which is involved in eliciting the antioxidant protection system including superoxidase dismutase (SOD) and possibly many other immune responses...
November 13, 2018: Developmental and Comparative Immunology
Maurilio Lopes Martins, Uelinton Manoel Pinto, Katharina Riedel, Maria Cristina Dantas Vanetti
The 16S rDNA of six psychrotrophic Enterobacteriaceae isolated from cold raw milk were sequenced and the isolate 039 was identified as Pantoea sp., isolates 059, 068, and 071 were identified as Hafnia alvei , 067 was identified as Enterobacter sp., and 099 was identified as Aeromonas hydrophila . They presented different spoilage potentials in milk with A. hydrophila 099 being the most deteriorative. Only Pantoea sp. 039 was not able to induce the quorum sensing monitor strains of acyl homoserine lactones (AHLs)...
2018: BioMed Research International
Xiao-Dong Li, Xin Li, Xiao-Ming Li, Gang-Ming Xu, Yang Liu, Bin-Gui Wang
Four new uncommon 20-nor-isopimarane diterpenoid epimers, aspewentins I-L ( 1 ⁻ 4 ), together with a new methylated derivative of 3 , aspewentin M ( 5 ), were isolated from the deep sea sediment-derived fungus Aspergillus wentii SD-310. The very similar structures of these epimers made the separation and purification procedures difficult. The structures of compounds 1 ⁻ 5 were illustrated based on spectroscopic analysis, and the absolute configurations of compounds 1 ⁻ 5 were unambiguously determined by the combination of NOESY, time-dependent density functional (TDDFT)-ECD calculations, and X-ray crystallographic analysis...
November 9, 2018: Marine Drugs
Ander Hernández Plágaro, Peter B Pearman, Vladimir R Kaberdin
Vibrio harveyi is a Gram-negative pathogenic bacterium ubiquitously present in natural aquatic systems. Although environmental adaptability in V. harveyi may be enabled by profound reprogramming of gene expression previously observed during responses to starvation, suboptimal temperatures and other stress factors, the key characteristics of V. harveyi transcripts and operons, such as their boundaries and size as well as location of small RNA genes, remain largely unknown. To reveal the main features of the V...
November 10, 2018: Genomics
Surapun Tepaamorndech, Kanittha Chantarasakha, Yutthana Kingcha, Sage Chaiyapechara, Metavee Phromson, Malinee Sriariyanun, Catherine P Kirschke, Liping Huang, Wonnop Visessanguan
The use of probiotics in aquaculture is a practical alternative to promote animal health and disease prevention. Meanwhile, this practice can also reduce the use of prophylactic antibiotics. The purpose of this study was to identify candidate probiotics that could control pathogen populations in host's gastrointestinal (GI) tract and stimulate host immunity in shrimp aquaculture. Bacillus aryabhattai TBRC8450, a bacterial strain isolated from the environment in a shrimp farm, has an antimicrobial activity against many pathogenic strains of Vibrio harveyi and V...
November 10, 2018: Fish & Shellfish Immunology
Yunji Xiu, Hongxiang Zhang, Shuangyan Wang, Tian Gan, Min Wei, Shun Zhou, Songlin Chen
Rac1 and Rac2, belonging to the small Rho GTPase family, play an important role during the immune responses. In this study, a Rac1 homolog (CsRac1) and a Rac2 homolog (CsRac2) were cloned from the Cynoglossus semilaevis. The full-length of CsRac1 and CsRac2 cDNA was 1219 bp and 1047 bp, respectively. Both CsRac1 and CsRac2 contain a 579 bp open reading frame (ORF) which encoding a 192 amino acids putative protein. The predicted molecular weight of CsRac1 and CsRac2 was 21.41 kDa and 21.35 kDa, and their theoretical pI was 8...
November 3, 2018: Fish & Shellfish Immunology
Min Wei, Wen-Teng Xu, Tian Gan, Lei Wang, Hong-Xiang Zhang, Fa-Zhen Zhao, Song-Lin Chen
STAT plays important roles in innate immunity during JAK/STAT signaling pathway, and STAT5 is particularly focused due to the existence of duplicated forms in fish and mammal. In Chinese tongue sole, stat5bl was suggested to be a candidate related to Vibrio harveyi resistance based on previous QTL screening. In this study, the full length of stat5bl cDNA was cloned and its expression patterns were analyzed. stat5bl was predominantly expressed in immune tissues, where the highest level was observed in liver, followed by skin and gill...
November 3, 2018: Fish & Shellfish Immunology
Tomasz Janek, Anna Krasowska, Żaneta Czyżnikowska, Marcin Łukaszewicz
Rhodococcus fascians BD8, isolated from Arctic soil, was found to produce biosurfactant when grown on n-hexadecane as the sole carbon source. The glycolipid product was identified as the trehalose lipid with a molecular mass of 848 g mol-1 . The purified biosurfactant reduced the surface tension of water from 72 to 34 mN m-1 . The critical micelle concentration of trehalose lipid was 0.140 mg mL-1 . To examine its potential for biomedical applications, the antimicrobial and antiadhesive activity of the biosurfactant was evaluated against several pathogenic microorganisms...
2018: Frontiers in Microbiology
Zhi-Qiang Du, Yue Wang, Hong-Yu Ma, Xiu-Li Shen, Kai Wang, Jie Du, Xiao-Dong Yu, Wen-Hong Fang, Xin-Cang Li
Crustins play important roles in defending against bacteria in the innate immunity system of crustaceans. In present study, we identified a crustin gene in Scylla paramamosain, which was named as SpCrus6. The ORF of SpCrus6 possessed a signal peptide sequence (SPS) at the N-terminus and a WAP domain at the C-terminus. And there were 5 Proline residues, 5 Glycine and 4 Cysteine residues between SPS and WAP domain in SpCrus6. These features indicated that SpCrus6 was a new member of crustin family. The SpCrus6 mRNA transcripts were up-regulated obviously after bacteria or virus challenge...
October 28, 2018: Fish & Shellfish Immunology
Yiqin Deng, Youlu Su, Songlin Liu, Zhixun Guo, Changhong Cheng, Hongling Ma, Jinjun Wu, Juan Feng, Chang Chen
Small non-coding RNAs (sRNAs) are important modulators of gene expression and are involved in the pathogenesis and survival of prokaryotes. However, few studies have been conducted with Vibrio alginolyticus , which limits our ability to probe the regulation of virulence and environmental adaptation by sRNAs in this opportunistic pathogen. In this study, the sRNA candidate srvg23535 was identified in V. alginolyticus ZJ-T. The precise transcript end, secondary structure, and sequence conservation were determined...
2018: Frontiers in Microbiology
Hiroaki Naka, Zachary L Reitz, Aneta M Jelowicki, Alison Butler, Margo G Haygood
In the original publication, third author's name was incorrectly published as Aneta L. Jelowicki.
October 2018: Journal of Biological Inorganic Chemistry: JBIC
François Delavat, Adeline Bidault, Vianney Pichereau, Christine Paillard
Vibrio campbellii BAA-1116 is renowned for its bioluminescence properties, and genetic tools are available to genetically track this strain. However, many other ecologically important V. harveyi strains exist, for which only few genetic tools are available. In this study, a rapid electroporation protocol was developed to transform replicative plasmids in various environmental V. harveyi and Pseudoalteromonas strains. Moreover, a mini-Tn7 delivery system was modified to site-specifically integrate mini-transposons in the genome of V...
October 2, 2018: Journal of Microbiological Methods
R Thirugnanasambandam, D Inbakandan, C Kumar, B Subashni, R Vasantharaja, L Stanley Abraham, N Ayyadurai, P Sriyutha Murthy, R Kirubagaran, S Ajmal Khan, T Balasubramanian
The pathogenicity of "Vibriosis" in shrimps imposes prominent menace to the sustainable growth of mariculture economy. Often the disease outbreak is associated speciously with Vibrio harveyi and its closely related species. The present study investigated the complete genome of the strain V. harveyi RT-6 to explore the molecular mechanism of pathogenesis. The genome of V. harveyi possesses a single chromosome of 6,374,398 bp in size, G + C content (44.7%) and 5730 protein coding genes. The reads of 1...
September 28, 2018: Molecular Phylogenetics and Evolution
Yosra A Helmy, Loic Deblais, Issmat I Kassem, Dipak Kathayat, Gireesh Rajashekara
Colibacillosis caused by avian pathogenic E. coli (APEC), is an economically important bacterial disease of poultry. APEC are a subgroup of extra intestinal pathogenic E. coli (ExPEC) and poultry are considered potential sources of foodborne ExPEC to humans. Currently, APEC infections in poultry are controlled by antibiotics and/or vaccination; however, their effect is limited due to emergence of antibiotic resistant strains and infections with heterologous serotypes. Therefore, novel approaches are needed...
2018: Virulence
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