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vibrio harveyi

Timothy J Green, Nachshon Siboni, William L King, Maurizio Labbate, Justin R Seymour, David Raftos
Marine heat waves are predicted to become more frequent and intense due to anthropogenically induced climate change, which will impact global production of seafood. Links between rising seawater temperature and disease have been documented for many aquaculture species, including the Pacific oyster Crassostrea gigas. The oyster harbours a diverse microbial community that may act as a source of opportunistic pathogens during temperature stress. We rapidly raised the seawater temperature from 20 °C to 25 °C resulting in an oyster mortality rate of 77...
August 10, 2018: Microbial Ecology
Hin-Chung Wong, Tzu-Yun Wang, Chu-Wen Yang, Chung-Tao Tang, Chingwen Ying, Chun-Hsiung Wang, Wei-Hau Chang
Vibrio parahaemolyticus is a human enteropathogenic bacterium and is also pathogenic to shrimp and finfish. In a search for a biocontrol agent for V. parahaemolyticus and other pathogenic Vibrio species, a lytic phage VP06 was isolated from oyster using V. parahaemolyticus as the host. VP06 is a Siphoviridae phage with a polyhedral head and a long tail. The genome sequence of VP06 was 75,893 nucleotides in length and the G+C content was 49%; a total of 101 CDSs were identified in VP06, of which 39 exhibited functional domains/motifs...
August 2, 2018: Research in Microbiology
Gang Liu, Zhangying Ye, Dezhao Liu, Jian Zhao, Elayaraja Sivaramasamy, Yale Deng, Songming Zhu
A 120-day feeding trial was conducted to evaluate the effect of different stocking densities on growth, the non-specific immunities, antioxidant status and digestive enzyme activities of Oreochromis niloticus fingerlings under a zero-water exchange biofloc system. Tilapias (0.51 ± 0.05 g) were randomly distributed in twelve tanks, each with 300 L water. The experimental design was completely randomized using three replications with four treatments 166 orgs m-3 (LD, low density), 333 orgs m-3 (MD, middle density) and 600 orgs m-3 (HD, high density) with glucose added as biofloc groups, and a clear water group without glucose added as a control 333 orgs m-3 ...
July 26, 2018: Fish & Shellfish Immunology
Keri A Lydon, Megan J Robertson, Erin K Lipp
The antimicrobial additive triclosan has been used in personal care products widely across the globe for decades. Triclosan resistance has been noted among Vibrio spp., but reports have been anecdotal and the extent of phenotypic triclosan resistance across the Vibrionaceae family has not been established . Here, triclosan resistance was determined for Vibrionaceae strains across nine distinct clades. Minimum inhibitory concentrations (MIC) were determined for 70 isolates from clinical ( n  = 6) and environmental sources ( n  = 64); only two were susceptible to triclosan...
2018: PeerJ
William L King, Cheryl Jenkins, Jeffrey Go, Nachshon Siboni, Justin R Seymour, Maurizio Labbate
The Pacific oyster, Crassostrea gigas, is a key commercial species that is cultivated globally. In recent years, disease outbreaks have heavily impacted C. gigas stocks worldwide, with many losses incurred during summer. A number of infectious agents have been associated with these summer mortality events, including viruses (particularly Ostreid herpesvirus 1, OsHV-1) and bacteria; however, cases where no known aetiological agent can be identified are common. In this study, we examined the microbiome of disease-affected and disease-unaffected C...
July 10, 2018: Microbial Ecology
Susana Llivisaca, Patricia Manzano, Jenny Ruales, José Flores, Joffre Mendoza, Esther Peralta, Juan M Cevallos-Cevallos
Fruits and leaves of Vaccinium spp. are known for their high content of bioactive compounds, but the chemical and biological characteristics of mortiño ( Vaccinium floribundum Kunth) have not been fully described. In this study, the levels of polyphenols, antioxidant capacity, anthocyanins, antimicrobial activity, and genetic variability were determined in mortiño plants. The Folin-Ciocalteu's, ABTS scavenging, pH differential, and well diffusion methods were used to evaluate the levels of polyphenols, antioxidant capacity, anthocyanins, and antimicrobial activity, respectively...
June 2018: Food Science & Nutrition
Sergio Alonso, M Carmen Castro, Margarita Berdasco, Inés García de la Banda, Xabier Moreno-Ventas, Alma Hernández de Rojas
With the increase of antimicrobial resistances due to the widespread use of antibiotics, the search of new probiotics to control aquaculture diseases has a growing public interest. The aim of this study was to isolate bacteria with antimicrobial effect from the gut of marine healthy fishes and select lactic acid bacteria (LAB) as potential probiotics, being strains considered as generally regarded as safe (GRAS) by the European Food Safety Agency (EFSA). Of a total of 45 Gram-positive strains with antimicrobial activity found in a screening of the gut microbiota of 13 marine fishes, nine were identified as LAB by 16S rRNA gene sequencing...
June 29, 2018: Probiotics and Antimicrobial Proteins
Shinya Monno, Hidehiro Sugiura, Hazuki Yamashita, Yusuke Kato, Kazuyuki Morimitu, Masayuki Imajoh
Vibrio harveyi strain GAN1709 was isolated from a diseased greater amberjack farmed in Nomi Bay, Japan. Here, we report the draft genome sequence of this strain, which comprises 6,265,473 bp, with a G+C content of 44.8%.
June 28, 2018: Genome Announcements
Darcy L McRose, Oliver Baars, Mohammad R Seyedsayamdost, François M M Morel
The secretion of small Fe-binding molecules called siderophores is an important microbial strategy for survival in Fe-limited environments. Siderophore production is often regulated by quorum sensing (QS), a microbial counting technique that allows organisms to alter gene expression based on cell density. However, the identity and quantities of siderophores produced under QS regulation are rarely studied in the context of their roles in Fe uptake. We investigated the link between QS, siderophores, and Fe uptake in the model marine organism Vibrio harveyi where QS is thought to repress siderophore production...
July 17, 2018: Proceedings of the National Academy of Sciences of the United States of America
Amandeep Kaur, Neena Capalash, Prince Sharma
BACKGROUND: Quorum sensing is a mechanism of cell to cell communication that requires the production and detection of signaling molecules called autoinducers. Although mesophilic bacteria is known to utilize this for synchronization of physiological processes such as bioluminescence, virulence, biofilm formation, motility and cell competency through signaling molecules (acyl homoserine lactones, AI-1; oligopeptides, peptide based system and furanosyl borate diester, AI-2), the phenomenon of quorum sensing in thermophiles is largely unknown...
June 28, 2018: BMC Microbiology
Sujeet Kumar, Mohmad Mashooq, Ravi Kumar Gandham, S V Alavandi, Viswas Konasagara Nagaleekar
Clostridium chauvoei causes fatal black quarter infection in cattle and buffaloes. The quorum sensing (QS) system, a bacterial cell to cell communication process, of the pathogen was characterized in the current study. The results indicated that C. chauvoei lacked luxS (autoinducer-2) based quorum sensing as detected by the sensor strain Vibrio harveyi BB170. This was supported by absence of luxS gene in C. chauvoei genome. However, the genomic analysis indicated the presence of agrBD system in all three genomes of C...
June 19, 2018: Anaerobe
Prapansak Srisapoome, Nawanith Klongklaew, Nontawith Areechon, Ratree Wongpanya
Anti-lipopolysaccharide factor (ALF) is an immune-related protein that is crucially involved in immune defense mechanisms against invading pathogens in crustaceans. In the current study, three different ALFs of giant river prawn (Mr-ALF3, Mr-ALF8 and Mr-ALF9) were discovered. Based on sequence analysis, Mr-ALF3 and Mr-ALF9 were identified as new members of ALFs in crustaceans (groups F and G, respectively). Structurally, each newly identified Mr-ALF contained three α-helices packed against a four-stranded β-sheet bearing the LPS-binding motif, which usually binds to the cell wall components of bacteria...
September 2018: Fish & Shellfish Immunology
Zhenjie Cao, Lu Wang, Yajing Xiang, Xiaocen Liu, Zhigang Tu, Yun Sun, Yongcan Zhou
The major histocompatibility complex (MHC) plays an important role in the vertebrate immune response to antigenic peptides, and it is essential for recognizing foreign pathogens in organisms. In this study, MHC class IIα (Trov-MHC IIα) from the golden pompano (Trachinotus ovatus) was first cloned and identified. The gene structure of Trov-MHC IIα was contained four exons and three introns. High levels of polymorphism were found in the exon 2 of Trov-MHC IIα. A total of 29 different MHC class IIα alleles with high polymorphism were identified from 80 individuals...
September 2018: Fish & Shellfish Immunology
Hai Trong Nguyen, Thuy Thi Thu Nguyen, Yan-Chun Chen, Hung Vu-Khac, Pei-Chi Wang, Shih-Chu Chen
Vibriosis is a severe infection occurring in many commercially important marine fish species. In this study, vaccines containing Vibrio harveyi recombinant outer membrane protein K (rOmpK), outer membrane protein U (rOmpU) and rOmpK-OmpU fusion protein in addition to the metabolizable MontanideTM ISA 763 A VG adjuvant were developed and evaluated in the orange-spotted grouper. The results indicate that recombinant V. harveyi protein-based vaccines resulted in a remarkably higher expression of IL-1β and IL-8 at 24 hr, and greater antibody production, as early as 2 weeks postimmunization...
June 8, 2018: Journal of Fish Diseases
Issei Nishiki, Takayuki Minami, Ayana Murakami, Truong Dinh Hoai, Atushi Fujiwara
Since 2011, high mortality rates and symptoms consistent with vibriosis have been observed in farmed amberjack (Seriola dumerili) in Japan. To identify 41 strains isolated from diseased amberjack, a multilocus sequence analysis using nine concatenated genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA, topA and 16S rRNA) was conducted. Twenty-seven strains were identified as Vibrio harveyi, suggesting an epidemic of V. harveyi infection in amberjack farms. Other strains were identified as Vibrio anguillarum, Vibrio owensii and Photobacterium damselae subsp...
June 7, 2018: Journal of Fish Diseases
Sivasubramanian Santhakumari, Rengarajan Jayakumar, Ravichandran Logalakshmi, Narayanan Marimuthu Prabhu, Abdul Kuthus Abdul Nazar, Shunmugiah Karutha Pandian, Arumugam Veera Ravi
This study unveils the in vitro and in vivo antibiofilm potential of 2,6-Di-tert-butyl-4-methylphenol (DTBMP) from Chroococcus turgidus against Vibrio spp. In the preliminary study, cell free culture supernatant (CFCS) of C. turgidus inhibited the violacein production in biomarker strain Chromobacterium violaceum and its mutant strain CV026 in a dose dependent manner. The effective biofilm inhibitory concentration (BIC) of pure compound DTBMP from C. turgidus was identified as 250 μg/ml concentration in tested Vibrio species...
May 25, 2018: International Journal of Food Microbiology
Luca De Vincenti, Yvana Glasenapp, Cristina Cattò, Federica Villa, Francesca Cappitelli, Jutta Papenbrock
BACKGROUND: Biofilms have great significance in healthcare-associated infections owing to their inherent tolerance and resistance to antimicrobial therapies. New approaches to prevent and treat unwanted biofilms are urgently required. To this end, three seagrass species (Enhalus acoroides, Halophila ovalis and Halodule pinifolia) collected in Vietnam and in India were investigated for their effects in mediating non-lethal interactions on sessile bacterial (Escherichia coli) and fungal (Candida albicans) cultures...
May 30, 2018: BMC Complementary and Alternative Medicine
Karolina Pierzynowska, Agata Rzeszótko, Aleksandra Blendowska, Ewa Wieczerzak, Sylwia Rodziewicz-Motowidło, Ewa Piotrowska, Grzegorz Węgrzyn
Flavonoids, polyphenolic compounds present in many food products, affect growth of different bacterial species when tested as purified or synthetic substances. They can also influence gene expression in human cells, like fibroblasts. Here, we asked if soy isoflavone extracts, commonly used in many products sold as anti-menopausal dietary supplements, influence bacterial growth similarly to a synthetic isoflavone, genistein. Four commercially available products were tested in amounts corresponding to genistein concentrations causing inhibition of growth of Vibrio harveyi (a model bacterium sensitive to this isoflavone) and Escherichia coli (a model bacterium resistant to genistein)...
2018: Acta Biochimica Polonica
Shichen Yu, Xiaoshan Zhu, Jin Zhou, Zhonghua Cai
Biofilms play an important role in the antibiotic resistance of encased bacteria, and biofilm formation is regulated by quorum sensing (QS). Inhibiting the QS system may, therefore, degrade the integrity of a biofilm and expose the bacterial pathogens within it to the deleterious effects of molecules such as antibiotics. Moreover, the use of QS inhibitors (QSIs) may provide a novel approach for treating bacterial infections of aquacultures. In the present study, the bacterium Proteus mirabilis was identified as a potential producer of QSIs...
April 2018: Royal Society Open Science
Ferdinand Sedlmayer, Dennis Hell, Marius Müller, David Ausländer, Martin Fussenegger
Quorum sensing is a promising target for next-generation anti-infectives designed to address evolving bacterial drug resistance. The autoinducer-2 (AI-2) is a key quorum-sensing signal molecule which regulates bacterial group behaviors and is recognized by many Gram-negative and Gram-positive bacteria. Here we report a synthetic mammalian cell-based microbial-control device that detects microbial chemotactic formyl peptides through a formyl peptide sensor (FPS) and responds by releasing AI-2. The microbial-control device was designed by rewiring an artificial receptor-based signaling cascade to a modular biosynthetic AI-2 production platform...
May 8, 2018: Nature Communications
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