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vibrio harveyi

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https://www.readbyqxmd.com/read/30291439/correction-to-amphi%C3%A2-enterobactin-commonly-produced-among-vibrio-campbellii-and-vibrio-harveyi-strains-can-be-taken-up-by-a-novel-outer-membrane-protein-fapa-that-also-can-transport-canonical-fe-iii-%C3%A2-enterobactin
#1
Hiroaki Naka, Zachary L Reitz, Aneta M Jelowicki, Alison Butler, Margo G Haygood
In the original publication, third author's name was incorrectly published as Aneta L. Jelowicki.
October 5, 2018: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/30287352/rapid-and-efficient-protocol-to-introduce-exogenous-dna-in-vibrio-harveyi-and-pseudoalteromonas-sp
#2
François Delavat, Adeline Bidault, Vianney Pichereau, Christine Paillard
Vibrio campbellii BAA-1116 is renowned for its bioluminescence properties, and genetic tools are available to genetically track this strain. However, many other ecologically important V. harveyi strains exist, for which only few genetic tools are available. In this study, a rapid electroporation protocol was developed to transform replicative plasmids in various environmental V. harveyi and Pseudoalteromonas strains. Moreover, a mini-Tn7 delivery system was modified to site-specifically integrate mini-transposons in the genome of V...
October 2, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/30273757/genomic-insights-of-vibrio-harveyi-rt-6-strain-from-infected-whiteleg-shrimp-litopenaeus-vannamei-using-illumina-platform
#3
R Thirugnanasambandam, D Inbakandan, C Kumar, B Subashni, R Vasantharaja, L Stanley Abraham, N Ayyadurai, P Sriyutha Murthy, R Kirubagaran, S Ajmal Khan, T Balasubramanian
The pathogenicity of "Vibriosis" in shrimps imposes prominent menace to the sustainable growth of mariculture economy. Often the disease outbreak is associated speciously with Vibrio harveyi and its closely related species. The present study investigated the complete genome of the strain V. harveyi RT-6 to explore the molecular mechanism of pathogenesis. The genome of V. harveyi possesses a single chromosome of 6,374,398 bp in size, G + C content (44.7%) and 5730 protein coding genes. The reads of 1...
September 28, 2018: Molecular Phylogenetics and Evolution
https://www.readbyqxmd.com/read/30270715/novel-small-molecule-modulators-of-quorum-sensing-in-avian-pathogenic-escherichia-coli-apec
#4
Yosra A Helmy, Loic Deblais, Issmat I Kassem, Dipak Kathayat, Gireesh Rajashekara
Colibacillosis caused by avian pathogenic E. coli (APEC), is an economically important bacterial disease of poultry. APEC are a subgroup of extra intestinal pathogenic E. coli (ExPEC) and poultry are considered potential sources of foodborne ExPEC to humans. Currently, APEC infections in poultry are controlled by antibiotics and/or vaccination; however, their effect is limited due to emergence of antibiotic resistant strains and infections with heterologous serotypes. Therefore, novel approaches to are needed...
September 29, 2018: Virulence
https://www.readbyqxmd.com/read/30255070/non-invasive-in-vivo-imaging-of-fluorescence-labeled-bacterial-distributions-in-aquatic-species
#5
S Ramachandran, S Thiyagarajan, G Dhinakar Raj, A Uma
In vivo imaging is becoming an advanced tool for noninvasive distribution of longitudinal small animals. However, the aquatic species have been limited to the optical imaging of noninvasively tracking on pathogen distribution. The purpose of this study was to develop shell-less fish and shrimp models of non-invasive in vivo imaging technique for visualization of pathogens. This experiment was utilized Escherichia coli, Edwardsiella tarda , Vibrio alginolyticus and Vibrio harveyi labeled with fluorescence probes to imaging bacterial distributions by IVIS Lumina LT system...
December 2017: International Journal of Veterinary Science and Medicine
https://www.readbyqxmd.com/read/30224438/surfing-motility-a-conserved-yet-diverse-adaptation-among-motile-bacteria
#6
Evelyn Sun, Sijie Liu, Robert E W Hancock
Bacterial rapid surfing motility is a novel surface adaptation of Pseudomonas aeruginosa in the presence of the glycoprotein, mucin. Here we show that other Gram-negative motile bacterial species including Escherichia coli , Salmonella enterica , Vibrio harveyi , Enterobacter cloacae , and Proteus mirabilis also exhibit the physical characteristics of surfing on the surface of agar plates containing 0.4% mucin, wherein surfing motility was generally more rapid and less dependent on medium viscosity than swimming motility...
September 17, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/30182304/multiplex-pcr-using-yead-and-16s-rrna-gene-to-identify-major-pathogens-in-vibriosis-of-litopenaeus-vannamei
#7
Yeong-Jong Han, Ara Jo, So-Won Kim, Hee-Eun Lee, Young Chul Kim, Hyun Do Jeong, Yung Hyun Choi, Suhkmann Kim, Hee-Jae Cha, Heui-Soo Kim
The Vibrio species causing major diseases in Litopenaeus vannamei are Vibrio harveyi, Vibrio alginolyticus, and Vibrio parahaemolyticus. For multiplex PCR primers, YeaD was used to detect the three Vibrio species. Bioinformatic analysis such as MultiPLX and primer-BLAST was used to design stable and species-specific multiplex PCR primers. Multiplex PCR results showed clear band patterns with bands at 185 bp for V. alginolyticus, 396 bp for V. harveyi, 805 bp for V. arahaemolyticus, and 596 bp for common Vibrio species...
September 4, 2018: Genes & Genomics
https://www.readbyqxmd.com/read/30166588/pirvp-genes-causing-ahpnd-identified-in-a-new-vibrio-species-vibrio-punensis-within-the-commensal-orientalis-clade
#8
Leda Restrepo, Bonny Bayot, Sebastián Arciniegas, Leandro Bajaña, Irma Betancourt, Fanny Panchana, Alejandro Reyes Muñoz
Acute hepatopancreatic necrosis disease (AHPND) has extended rapidly, causing alarming shrimp mortalities. Initially, the only known causative agent was Vibrio parahaemolyticus carrying a plasmid coding for the mortal toxins PirVP . Recently, it has been found that the plasmid and hence the disease, could be transferred among members of the Harveyi clade. The current study performs a genomic characterization of an isolate capable of developing AHPND in shrimp. Mortality studies and molecular and histopathological analyses showed the infection capacity of the strain...
August 30, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30158916/comparative-genomic-analysis-of-vibrio-diabolicus-and-six-taxonomic-synonyms-a-first-look-at-the-distribution-and-diversity-of-the-expanded-species
#9
Jeffrey W Turner, James J Tallman, Amanda Macias, Lee J Pinnell, Nicole C Elledge, Danial Nasr Azadani, William B Nilsson, Rohinee N Paranjpye, E V Armbrust, Mark S Strom
Vibrio is a diverse genus of Gammaproteobacteria autochthonous to marine environments worldwide. Vibrio diabolicus and V. antiquarius were originally isolated from deep-sea hydrothermal fields in the East Pacific Rise. These species are closely related to members of the Harveyi clade (e.g., V. alginolyticus and V. parahaemolyticus ) that are commonly isolated from coastal systems. This study reports the discovery and draft genome sequence of a novel isolate ( Vibrio sp. 939) cultured from Pacific oysters ( Crassostrea gigas )...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30135989/amphi-enterobactin-commonly-produced-among-vibrio-campbellii-and-vibrio-harveyi-strains-can-be-taken-up-by-a-novel-outer-membrane-protein-fapa-that-also-can-transport-canonical-fe-iii-enterobactin
#10
Hiroaki Naka, Zachary L Reitz, Aneta L Jelowicki, Alison Butler, Margo G Haygood
Vibrio campbellii BAA-1116 (formerly Vibrio harveyi) is a model organism for quorum sensing study and produces the siderophores anguibactin and amphi-enterobactin. This study examined the mechanisms and specificity of siderophore uptake in V. campbellii and V. harveyi, and surveyed the diversity of siderophore production in V. campbellii and V. harveyi strains. The amphi-enterobactin gene cluster of BAA-1116 harbors a gene, named fapA, that is a homologue of genes encoding Fe(III)-siderophore-specific outer membrane receptors...
August 22, 2018: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/30125700/effects-of-dietary-administration-of-lactococcus-lactis-hnl12-on-growth-innate-immune-response-and-disease-resistance-of-humpback-grouper-cromileptes-altivelis
#11
Yun Sun, Mingwang He, Zhenjie Cao, Zhenyu Xie, Chunsheng Liu, Shifeng Wang, Weiliang Guo, Xiang Zhang, Yongcan Zhou
Lactic acid bacteria are a common group of probiotics that have been widely studied and used in aquaculture. In the present study, we isolated Lactococcus lactis HNL12 from the gut of wild humpback grouper (Cromileptes altivelis) and explored its probiotic properties. For this purpose, L. lactis HNL12 was added to the commercial fish feed. The results showed that HNL12 had high auto-aggregation ability and strong tolerance to simulated gastrointestinal stress. When C. altivelis consumed a diet containing 0 (control), 106 , 108 , or 1010  CFU/g HNL12 for four weeks, all of the groupers fed a diet with HNL12 had significantly increased percent weight gain (PWG), especially those fed with 108  CFU/g, which had a PWG of 231...
August 18, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30097682/simulated-marine-heat-wave-alters-abundance-and-structure-of-vibrio-populations-associated-with-the-pacific-oyster-resulting-in-a-mass-mortality-event
#12
Timothy J Green, Nachshon Siboni, William L King, Maurizio Labbate, Justin R Seymour, David Raftos
Marine heat waves are predicted to become more frequent and intense due to anthropogenically induced climate change, which will impact global production of seafood. Links between rising seawater temperature and disease have been documented for many aquaculture species, including the Pacific oyster Crassostrea gigas. The oyster harbours a diverse microbial community that may act as a source of opportunistic pathogens during temperature stress. We rapidly raised the seawater temperature from 20 °C to 25 °C resulting in an oyster mortality rate of 77...
August 10, 2018: Microbial Ecology
https://www.readbyqxmd.com/read/30077624/characterization-of-a-lytic-vibriophage-vp06-of-vibrio-parahaemolyticus
#13
Hin-Chung Wong, Tzu-Yun Wang, Chu-Wen Yang, Chung-Tao Tang, Chingwen Ying, Chun-Hsiung Wang, Wei-Hau Chang
Vibrio parahaemolyticus is a human enteropathogenic bacterium and is also pathogenic to shrimp and finfish. In a search for a biocontrol agent for V. parahaemolyticus and other pathogenic Vibrio species, a lytic phage VP06 was isolated from oyster using V. parahaemolyticus as the host. VP06 is a Siphoviridae phage with a polyhedral head and a long tail. The genome sequence of VP06 was 75,893 nucleotides in length and the G + C content was 49%; a total of 101 CDSs were identified in VP06, of which 39 exhibited functional domains/motifs...
August 2, 2018: Research in Microbiology
https://www.readbyqxmd.com/read/30056209/influence-of-stocking-density-on-growth-digestive-enzyme-activities-immune-responses-antioxidant-of-oreochromis-niloticus-fingerlings-in-biofloc-systems
#14
Gang Liu, Zhangying Ye, Dezhao Liu, Jian Zhao, Elayaraja Sivaramasamy, Yale Deng, Songming Zhu
A 120-day feeding trial was conducted to evaluate the effect of different stocking densities on growth, the non-specific immunities, antioxidant status and digestive enzyme activities of Oreochromis niloticus fingerlings under a zero-water exchange biofloc system. Tilapias (0.51 ± 0.05 g) were randomly distributed in twelve tanks, each with 300 L water. The experimental design was completely randomized using three replications with four treatments 166 orgs m-3 (LD, low density), 333 orgs m-3 (MD, middle density) and 600 orgs m-3 (HD, high density) with glucose added as biofloc groups, and a clear water group without glucose added as a control 333 orgs m-3 ...
October 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/30013840/patterns-of-triclosan-resistance-in-vibrionaceae
#15
Keri A Lydon, Megan J Robertson, Erin K Lipp
The antimicrobial additive triclosan has been used in personal care products widely across the globe for decades. Triclosan resistance has been noted among Vibrio spp., but reports have been anecdotal and the extent of phenotypic triclosan resistance across the Vibrionaceae family has not been established . Here, triclosan resistance was determined for Vibrionaceae strains across nine distinct clades. Minimum inhibitory concentrations (MIC) were determined for 70 isolates from clinical ( n  = 6) and environmental sources ( n  = 64); only two were susceptible to triclosan...
2018: PeerJ
https://www.readbyqxmd.com/read/29987529/characterisation-of-the-pacific-oyster-microbiome-during-a-summer-mortality-event
#16
William L King, Cheryl Jenkins, Jeffrey Go, Nachshon Siboni, Justin R Seymour, Maurizio Labbate
The Pacific oyster, Crassostrea gigas, is a key commercial species that is cultivated globally. In recent years, disease outbreaks have heavily impacted C. gigas stocks worldwide, with many losses incurred during summer. A number of infectious agents have been associated with these summer mortality events, including viruses (particularly Ostreid herpesvirus 1, OsHV-1) and bacteria; however, cases where no known aetiological agent can be identified are common. In this study, we examined the microbiome of disease-affected and disease-unaffected C...
July 10, 2018: Microbial Ecology
https://www.readbyqxmd.com/read/29983956/chemical-antimicrobial-and-molecular-characterization-of-morti%C3%A3-o-vaccinium-floribundum-kunth-fruits-and-leaves
#17
Susana Llivisaca, Patricia Manzano, Jenny Ruales, José Flores, Joffre Mendoza, Esther Peralta, Juan M Cevallos-Cevallos
Fruits and leaves of Vaccinium spp. are known for their high content of bioactive compounds, but the chemical and biological characteristics of mortiño ( Vaccinium floribundum Kunth) have not been fully described. In this study, the levels of polyphenols, antioxidant capacity, anthocyanins, antimicrobial activity, and genetic variability were determined in mortiño plants. The Folin-Ciocalteu's, ABTS scavenging, pH differential, and well diffusion methods were used to evaluate the levels of polyphenols, antioxidant capacity, anthocyanins, and antimicrobial activity, respectively...
June 2018: Food Science & Nutrition
https://www.readbyqxmd.com/read/29959637/isolation-and-partial-characterization-of-lactic-acid-bacteria-from-the-gut-microbiota-of-marine-fishes-for-potential-application-as-probiotics-in-aquaculture
#18
Sergio Alonso, M Carmen Castro, Margarita Berdasco, Inés García de la Banda, Xabier Moreno-Ventas, Alma Hernández de Rojas
With the increase of antimicrobial resistances due to the widespread use of antibiotics, the search of new probiotics to control aquaculture diseases has a growing public interest. The aim of this study was to isolate bacteria with antimicrobial effect from the gut of marine healthy fishes and select lactic acid bacteria (LAB) as potential probiotics, being strains considered as generally regarded as safe (GRAS) by the European Food Safety Agency (EFSA). Of a total of 45 Gram-positive strains with antimicrobial activity found in a screening of the gut microbiota of 13 marine fishes, nine were identified as LAB by 16S rRNA gene sequencing...
June 29, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29954908/draft-genome-sequence-of-vibrio-harveyi-strain-gan1709-isolated-from-diseased-greater-amberjack-seriola-dumerili-farmed-in-japan
#19
Shinya Monno, Hidehiro Sugiura, Hazuki Yamashita, Yusuke Kato, Kazuyuki Morimitu, Masayuki Imajoh
Vibrio harveyi strain GAN1709 was isolated from a diseased greater amberjack farmed in Nomi Bay, Japan. Here, we report the draft genome sequence of this strain, which comprises 6,265,473 bp, with a G+C content of 44.8%.
June 28, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29954861/quorum-sensing-and-iron-regulate-a-two-for-one-siderophore-gene-cluster-in-vibrio-harveyi
#20
Darcy L McRose, Oliver Baars, Mohammad R Seyedsayamdost, François M M Morel
The secretion of small Fe-binding molecules called siderophores is an important microbial strategy for survival in Fe-limited environments. Siderophore production is often regulated by quorum sensing (QS), a microbial counting technique that allows organisms to alter gene expression based on cell density. However, the identity and quantities of siderophores produced under QS regulation are rarely studied in the context of their roles in Fe uptake. We investigated the link between QS, siderophores, and Fe uptake in the model marine organism Vibrio harveyi where QS is thought to repress siderophore production...
July 17, 2018: Proceedings of the National Academy of Sciences of the United States of America
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