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Outer membrane vesicle

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https://www.readbyqxmd.com/read/30507089/induction-effects-of-bacteroides-fragilis-derived-outer-membrane-vesicles-on-toll-like-receptor-2-toll-like-receptor-4-genes-expression-and-cytokines-concentration-in-human-intestinal-epithelial-cells
#1
Sara Ahmadi Badi, S Hohreh Khatami, S Hiva Irani, Seyed Davar Siadat
Objective: Gastrointestinal (GI) tract, like other mucosal surface, is colonized with a microbial population known as gut microbiota. Outer membrane vesicles (OMVs) which are produced by gram negative bacteria could be sensed by Toll like receptors (TLRs). The interaction between gut microbiota and TLRs affects homeostasis and immune responses. In this study, we evaluated TLR2, TLR4 genes expression and cytokines concentration in Caco-2 cell line treated with Bacteroides fragilis (B. fragilis) and its OMVs...
April 2019: Cell Journal
https://www.readbyqxmd.com/read/30504226/-listeria-monocytogenes-virulence-factors-including-listeriolysin-o-are-secreted-in-biologically-active-extracellular-vesicles
#2
Carolina Coelho, Lisa C Brown, Maria Maryam, Raghav Vij, Daniel Fq Smith, Meagan C Burnet, Jennifer E Kyle, Heino M Heyman, Jasmine Ramirez, Rafael Prados-Rosales, Gregoire Lauvau, Ernesto S Nakayasu, Nathan R Brady, Anne Hamacher-Brady, Isabelle Coppens, Arturo Casadevall
Outer membrane vesicles produced by Gram-negative bacteria have been studied for half a century but the possibility that Gram-positive bacteria secreted extracellular vesicles (EVs) was not pursued until recently due to the assumption that the thick peptidoglycan cell wall would prevent their release to the environment. However, following their discovery in fungi, which also have cell walls, EVs have now been described for a variety of Gram-positive bacteria. EVs purified from Gram-positive bacteria are implicated in virulence, toxin release and transference to host cells, eliciting immune responses, and spread of antibiotic resistance...
November 30, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/30503535/cooperative-induction-of-ordered-peptide-and-fatty-acid-aggregates
#3
Radoslaw Bomba, Witek Kwiatkowski, Antoni Sánchez-Ferrer, Roland Riek, Jason Greenwald
Interactions between biological membranes and disease-associated amyloids are well documented, and their prevalence suggests that an inherent affinity exists between these molecular assemblies. Our interest in the molecular origins of life have led us to investigate the nature of such interactions in the context of their molecular predecessors (i.e., vesicle-forming amphiphiles and small peptides). Under certain conditions, amyloidogenic peptides or fatty acids are each able to form ordered structures on their own; however, we report here on their cooperative assembly into novel, to our knowledge, highly ordered structures...
November 10, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/30489062/kill-the-real-with-the-fake-eliminate-intracellular-staphylococcus-aureus-using-nanoparticle-coated-with-its-extracellular-vesicle-membrane-as-active-targeting-drug-carrier
#4
Feng Gao, Lulu Xu, Binqian Yang, Feng Fan, Lihua Yang
Staphylococcus aureus bacteremia is one of the most serious bacterial infection worldwide. Most complications of S. aureus bacteremia arise because the pathogen can survive inside host phagocytes especially macrophages, which makes elimination of intracellular S. aureus key to clinical success. Unfortunately, most antibiotics have poor cellular penetration capacity, which necessitates intracellular delivery of antibiotics. We herein use nanoparticle coated with membrane of extracellular vesicle secreted by S...
November 29, 2018: ACS Infectious Diseases
https://www.readbyqxmd.com/read/30488570/helicobacter-pylori-growth-stage-determines-the-size-protein-composition-and-preferential-cargo-packaging-of-outer-membrane-vesicles
#5
Lauren Zavan, Natalie J Bitto, Ella L Johnston, David W Greening, Maria Kaparakis-Liaskos
Gram-negative bacteria release outer membrane vesicles (OMVs) as part of their normal growth that contain a range of cargo from their parent bacterium, including DNA, RNA, and proteins. The protein content of OMVs is suggested to be similar in composition to various sub-cellular locations of their parent bacterium. However, very little is known regarding the effect of bacterial growth stage on the size, content and selective packaging of proteins into OMVs. In this study, we examined the global proteome of Helicobacter pylori and their OMVs throughout bacterial growth to determine if bacterial growth stage affected OMV cargo composition...
November 28, 2018: Proteomics
https://www.readbyqxmd.com/read/30468729/interaction-of-artepillin-c-with-model-membranes-effects-of-ph-and-ionic-strength
#6
Wallance Moreira Pazin, Gilia Cristine Marques Ruiz, Osvaldo Novais de Oliveira, Carlos José Leopoldo Constantino
Artepillin C is the major constituent of green propolis, one of the most consumed products in popular medicine owing to its therapeutic effects, including antitumor activity. Artepillin C differs from other cinnamic acid derivatives due to the presence of two prenylated groups in its structure, believed to enhance access to the cell membrane and resulting in pharmacological activity. The membrane outer leaflet of tumor cells is exposed to an acidic extracellular environment, which could modulate the protonation state of antitumor drugs and hence their interaction with the cell membrane...
November 20, 2018: Biochimica et biophysica acta. Biomembranes
https://www.readbyqxmd.com/read/30464758/enhanced-biofilm-formation-and-membrane-vesicle-release-by-escherichia-coli-expressing-a-commonly-occurring-plasmid-gene-kil
#7
Ryoma Nakao, Si Lhyam Myint, Sun Nyunt Wai, Bernt Eric Uhlin
Escherichia coli is one of the most prevalent microorganisms forming biofilms on indwelling medical devices, as well as a representative model to study the biology and ecology of biofilms. Here, we report that a small plasmid gene, kil , enhances biofilm formation of E. coli . The kil gene is widely conserved among naturally occurring colicinogenic plasmids such as ColE1 plasmid, and is also present in some plasmid derivatives used as cloning vectors. First, we found that overexpression of the kil gene product dramatically increased biofilm mass enriched with extracellular DNA in the outer membrane-compromised strain RN102, a deep rough LPS mutant E...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30459769/outer-membrane-vesicle-associated-o-antigen-a-crucial-component-for-protecting-against-bordetella-parapertussis-infection
#8
Daniela Bottero, María Eugenia Zurita, María Emilia Gaillard, Francisco Carriquiriborde, Pablo Martin Aispuro, Maia Elizagaray, Erika Bartel, Celina Castuma, Daniela Hozbor
Bordetella parapertussis is a respiratory-disease pathogen producing symptomatology similar to that of pertussis but of underestimated incidence and with no specific vaccine existing. We recently designed a vaccine candidate from B. parapertussis outer-membrane vesicles (OMVs) that proved to be safe and protective in a murine-infection model. Based on protection recently reported for the B. parapertussis O antigen in aqueous solution, we assessed here whether the B. parapertussis O-antigen-containing lipopolysaccharide (BppLPS-O+ ) embedded in the membranes, as present in B...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/30451931/campylobacter-jejuni-bile-exposure-influences-outer-membrane-vesicles-protein-content-and-bacterial-interaction-with-epithelial-cells
#9
Nayyer Taheri, A K M Firoj Mahmud, Linda Sandblad, Maria Fällman, Sun Nyunt Wai, Anna Fahlgren
Campylobacter jejuni is a prevalent human pathogen and a major cause of bacterial gastroenteritis in the world. In humans, C. jejuni colonizes the intestinal tract and its tolerance to bile is crucial for bacteria to survive and establish infection. C. jejuni produces outer membrane vesicles (OMVs) which have been suggested to be involved in virulence. In this study, the proteome composition of C. jejuni OMVs in response to low concentration of bile was investigated. We showed that exposure of C. jejuni to low concentrations of bile, similar to the concentration in cecum, induced significant changes in the protein profile of OMVs released during growth without affecting the protein profile of the bacteria...
November 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30448065/monocyte-activation-test-to-reliably-measure-the-pyrogenic-content-of-a-vaccine-an-in-vitro-pyrogen-test-to-overcome-in-vivo-limitations
#10
Sara Valentini, Giovanna Santoro, Federica Baffetta, Sara Franceschi, Marilena Paludi, Elisa Brandini, Leonardo Gherardini, Davide Serruto, Barbara Capecchi
Pyrogen content is one of the critical quality attributes impacting the safety of a product, and there is an increasing need for assays that can reliably measure this attribute in vaccines. The Limulus amebocyte lysate (LAL) assay and the rabbit pyrogen test (RPT) are the canonical animal-based pyrogen tests currently used to release vaccines; however, there are several drawbacks associated with these tests when applied to Bexsero, intrinsically pyrogenic product, containing a meningococcal Outer Membrane Vesicle component...
November 14, 2018: Vaccine
https://www.readbyqxmd.com/read/30441886/erratum-to-outer-membrane-vesicles-derived-from-salmonella-enteritidis-protect-against-the-virulent-wild-type-strain-infection-in-a-mouse-model
#11
Qiong Liu, Jie Yi, Kang Liang, Xiangmin Zhang, Qing Liu
This erratum is being published to correct the error of Fig. 1B in page 1522. The previous Fig. 1B should be replaced with the below new Fig. 1B. The corrected version of this article is available in the Archive (http://jmb.or.kr).
October 28, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30441350/automatic-planar-asymmetric-lipid-bilayer-membrane-formation-toward-biological-high-throughput-assay
#12
Masahide Gotanda, Koki Kamiya, Toshihisa Osaki, Satoshi Fujii, Nobuo Misawa, Norihisa Miki, Shoji Takeuchi
This paper describes automation of planar lipid bilayer formation by introducing a stepping motor to a microfluidic device. Planar lipid bilayers or lipid vesicles are useful to understand biological reactions and to investigate the interaction between lipids and proteins. Therefore, to acquire large amount of the information, high-throughput production of planar lipid bilayers or giant vesicles (GVs) is necessary. The droplet split-and-contact method, which enhances the efficiencies of both planar lipid bilayer formation and GV generation, needs to be automated for increasing the throughput...
July 2018: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/30416985/vaccination-with-a-fat1-derived-b-cell-epitope-combined-with-tumor-specific-b-and-t-cell-epitopes-elicits-additive-protection-in-cancer-mouse-models
#13
Alberto Grandi, Laura Fantappiè, Carmela Irene, Silvia Valensin, Michele Tomasi, Simone Stupia, Riccardo Corbellari, Elena Caproni, Ilaria Zanella, Samine J Isaac, Luisa Ganfini, Luca Frattini, Enrico König, Assunta Gagliardi, Simona Tavarini, Chiara Sammicheli, Matteo Parri, Guido Grandi
Human FAT1 is overexpressed on the surface of most colorectal cancers (CRCs) and in particular a 25 amino acid sequence (D8) present in one of the 34 cadherin extracellular repeats carries the epitope recognized by mAb198.3, a monoclonal antibody which partially protects mice from the challenge with human CRC cell lines in xenograft mouse models. Here we present data in immune competent mice demonstrating the potential of the D8-FAT1 epitope as CRC cancer vaccine. We first demonstrated that the mouse homolog of D8-FAT1 (mD8-FAT1) is also expressed on the surface of CT26 and B16F10 murine cell lines...
2018: Frontiers in Oncology
https://www.readbyqxmd.com/read/30409143/helicobacter-pylori-outer-membrane-vesicles-involvement-in-the-infection-development-and-helicobacter-pylori-related-diseases
#14
REVIEW
Magdalena Chmiela, Natalia Walczak, Karolina Rudnicka
Helicobacter pylori - (H. pylori) play a role in the pathogenesis of gastritis, gastric and duodenal ulcers as well as gastric cancer. A possible involvement of outer membrane vesicles (OMVs) produced by H. pylori in the distribution of bacterial antigens through the gastric epithelial barrier and their role in the development of local and systemic host inflammatory and immune responses has been suggested. OMVs contain various biologically active compounds, which internalize into host cells affecting signaling pathways and promoting apoptosis of gastric epithelial and immunocompetent cells...
November 8, 2018: Journal of Biomedical Science
https://www.readbyqxmd.com/read/30404931/surface-exposure-and-packing-of-lipoproteins-into-outer-membrane-vesicles-are-coupled-processes-in-bacteroides
#15
Ezequiel Valguarnera, Nichollas E Scott, Philippe Azimzadeh, Mario F Feldman
Outer membrane vesicles (OMVs) are spherical structures derived from the outer membranes (OMs) of Gram-negative bacteria. Bacteroides spp. are prominent components of the human gut microbiota, and OMVs produced by these species are proposed to play key roles in gut homeostasis. OMV biogenesis in Bacteroides is a poorly understood process. Here, we revisited the protein composition of Bacteroides thetaiotaomicron OMVs by mass spectrometry. We confirmed that OMVs produced by this organism contain large quantities of glycosidases and proteases, with most of them being lipoproteins...
November 7, 2018: MSphere
https://www.readbyqxmd.com/read/30397270/types-and-origins-of-bacterial-membrane-vesicles
#16
REVIEW
Masanori Toyofuku, Nobuhiko Nomura, Leo Eberl
Most bacteria release membrane vesicles (MVs) that contain specific cargo molecules and have diverse functions, including the transport of virulence factors, DNA transfer, interception of bacteriophages, antibiotics and eukaryotic host defence factors, cell detoxification and bacterial communication. MVs not only are abundant in nature but also show great promise for applications in biomedicine and nanotechnology. MVs were first discovered to originate from controlled blebbing of the outer membrane of Gram-negative bacteria and are therefore often called outer-membrane vesicles (OMVs)...
November 5, 2018: Nature Reviews. Microbiology
https://www.readbyqxmd.com/read/30384271/protective-effect-of-epigallocatechin-gallate-on-human-erythrocytes
#17
José R Colina, Mario Suwalsky, Marcela Manrique-Moreno, Karla Petit, Luis F Aguilar, Malgorzata Jemiola-Rzeminska, Kazimierz Strzalka
The interactions and the protective effect of epigallocatechin gallate (EGCG) on human erythrocytes (RBC) and molecular models of its membrane were investigated. The latter consisted of bilayers built- up of dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine (DMPE), representative of phospholipid classes located in the outer and inner monolayers of the human erythrocyte membrane, respectively. X-ray diffraction and differential scanning calorimetry experiments showed that EGCG induced significant structural and thermotropic perturbations in multilayers and vesicles of DMPC; however, these effects were not observed in DMPE...
October 17, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/30380823/-escherichia-coli-derived-outer-membrane-vesicles-deliver-galactose-1-phosphate-uridyltransferase-and-yield-partial-protection-against-actinobacillus-pleuropneumoniae-in-mice
#18
Keji Quan, Zhuang Zhu, Sanjie Cao, Fei Zhang, Chang Miao, Xintian Wen, Xiaobo Huang, Yiping Wen, Rui Wu, Qigui Yan, Yong Huang, Xiaoping Ma, Xinfeng Han, Qin Zhao
In our previous studies, we have identified several in vivo -induced antigens and evaluated their potential as subunit vaccine candidates in a murine model, in which the recombinant protein GalT showed the most potent immunogenicity and immunoprotective efficacy against Actinobacillus pleuropneumoniae . To exploit a more efficient way of delivering GalT proteins, in this study, we employed the widely studied E. coli outer membrane vesicles (OMVs) as a platform to deliver GalT protein and performed the vaccine trial using the recombinant GalT-OMVs in murine model...
October 19, 2018: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30369908/outer-membrane-vesicles-derived-from-escherichia-coli-regulate-neutrophil-migration-by-induction-of-endothelial-il-8
#19
Jaewook Lee, Yae Jin Yoon, Ji Hyun Kim, Nhung Thi Hong Dinh, Gyeongyun Go, Sookil Tae, Kyong-Su Park, Hyun Taek Park, Changjin Lee, Tae-Young Roh, Dolores Di Vizio, Yong Song Gho
Outer membrane vesicles (OMVs) are spherical, proteolipid nanostructures that are constitutively released by Gram-negative bacteria including Escherichia coli . Although it has been shown that administration of E. coli OMVs stimulates a strong pulmonary inflammatory response with infiltration of neutrophils into the lungs in vivo , the mechanism of E. coli OMV-mediated neutrophil recruitment is poorly characterized. In this study, we observed significant infiltration of neutrophils into the mouse lung tissues in vivo , with increased expression of the neutrophil chemoattractant CXCL1, a murine functional homolog of human IL-8, on intraperitoneal administration of E...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30358793/resolving-the-structural-interactions-between-antimicrobial-peptides-and-lipid-membranes-using-small-angle-scattering-methods-the-case-of-indolicidin
#20
Josefine Eilsø Nielsen, Victoria Ariel Bjørnestad, Reidar Lund
Using small angle X-ray and neutron scattering (SAXS/SANS) and detailed theoretical modelling we have elucidated the structure of the antimicrobial peptide, indolicidin, and the interaction with model lipid membranes of different anionic lipid compositions mimicking typical charge densities found in the cytoplasmic membrane of bacteria. First, we show that indolicidin displays a predominantly disordered, random chain conformation in solution with a small fraction (≈1%) of fiber-like nanostructures that are not dissolved at higher temperatures...
November 7, 2018: Soft Matter
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