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https://www.readbyqxmd.com/read/30107327/impact-of-effective-microorganisms-on-the-transfer-of-radioactive-cesium-into-lettuce-and-barley-biomass
#1
Aleksander Nikolaevich Nikitin, Ihar Anatoljevich Cheshyk, Galina Zenonovna Gutseva, Elena Aleksandrovna Tankevich, Masaki Shintani, Shuichi Okumoto
Soil microorganisms play an important role in determining the physical and chemical properties of soils. Soil microorganisms have both direct and indirect effects on the physical and chemical states of radionuclides and their availability for uptake by plant roots. Controlling the soil microorganisms to immobilize radionuclides is a promising strategy to reduce the content of radionuclides in the food chain. In this study, we evaluated the impact of effective microorganisms (EM) comprising lactic-acid bacteria, photosynthetic bacteria, and yeast on the transfer of 137 Cs into the aboveground biomass of barley and lettuce...
August 11, 2018: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/30107218/combination-of-3d-printing-technologies-and-compressed-tablets-for-preparation-of-riboflavin-floating-tablet-in-device-tid-systems
#2
Junhui Fu, Hui Yin, Xiang Yu, Cong Xie, Heliu Jiang, Yiguang Jin, Fugeng Sheng
Gastric floating tablets have the functions of long-term gastric retention, sustained release and improving bioavailability though the floating time and sustained release are usually not satisfied. Here we designed a novel gastric floating system by combining compressed tablets with 3D printed devices, wherein a riboflavin tablet was contained into a device, named as tablet-in-device (TiD) systems. Commercial poly(lactic acid) filaments were used for 3D printing of the body and cap of the device. Four types of TiD systems were prepared, involving non-net, centrally symmetric double-net (including a peripheral sealed air-filled chamber and a net-on-both-sides chamber), single-net (including a sealed air-filled chamber and a net-on-one-side chamber), and eccentric double-net (including an eccentric air-filled chamber)...
August 11, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/30105738/in-situ-gel-forming-system-for-dual-bmp-2-and-17%C3%AE-estradiol-controlled-release-for-bone-regeneration-in-osteoporotic-rats
#3
Elisabet Segredo-Morales, Ricardo Reyes, María Rosa Arnau, Araceli Delgado, Carmen Évora
The aim of this study was to evaluate the bone regeneration capacity of a Pluronic® F127 (P)-Tetronic®1307 (T) and α-cyclodextrin (CD) supramolecular gel (P-T-CD) in a 11/7/7 ratio containing BMP-2 and 17β-estradiol pre-encapsulated in poly-lactide-co-glycolide (PLGA) and poly-lactic acid (PLA-S) microspheres, respectively. Ovariectomy combined with dexamethasone treatment was used to induce an osteoporotic (OP) animal model of calvaria critical size defect in female rats to test the system. The two active substances showed a biphasic in vitro release profile characterized by an initial fast phase followed by a slow and prolonged release...
August 13, 2018: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/30105525/comparison-between-fluorescent-probe-and-ion-selective-electrode-methods-for-intracellular-ph-determination-in-leuconostoc-mesenteroides
#4
Duried Alwazeer, Christophe Riondet, Rémy Cachon
The intracellular pH (pHin ) of Leuconostoc mesenteroides subsp. mesenteroides 19D was evaluated by two different methods, fluorescent probe and ion-selective electrode. Two fluorescent probes 5 (and-6)-carboxyfluorescein diacetate succinimidyl ester (cFDASE) and 5 (and-6)-carboxy-2',7'-dichlorofluorescein diacetate succinimidyl ester (cDCFDASE) were tested to evaluate the intracellular pH (pHin ) of living cells at a medium pH (pHex ) ranged from 5.0 to 6.5. Salicylic acid was used as a probe for the ion-selective electrode method...
August 13, 2018: Current Microbiology
https://www.readbyqxmd.com/read/30104643/plasma-metabolomic-analysis-in-mature-female-common-bottlenose-dolphins-profiling-the-characteristics-of-metabolites-after-overnight-fasting-by-comparison-with-data-in-beagle-dogs
#5
Miwa Suzuki, Motoi Yoshioka, Yoshito Ohno, Yuichiro Akune
The present study was aimed at determining the characteristics of plasma metabolites in bottlenose dolphins to provide a greater understanding of their metabolism and to obtain information for the health management of cetaceans. Capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) and liquid chromatograph-time-of-flight mass spectrometry (LC-TOFMS) were conducted on plasma samples after overnight fasting from three common bottlenose dolphins as well as three beagle dogs (representative terrestrial carnivores) for comparison...
August 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30104505/risperidone-controlled-release-microspheres-based-on-poly-lactic-acid-poly-propylene-adipate-novel-polymer-blends-appropriate-for-long-acting-injectable-formulations
#6
Stavroula Nanaki, Panagiotis Barmpalexis, Alexandros Iatrou, Evi Christodoulou, Margaritis Kostoglou, Dimitrios N Bikiaris
The present study evaluates the preparation of risperidone controlled release microspheres as appropriate long-acting injectable formulations based on a series of novel biodegradable and biocompatible poly(lactic acid)⁻poly(propylene adipate) (PLA/PPAd) polymer blends. Initially, PPAd was synthesized using a two-stage melt polycondensation method (esterification and polycondensation) and characterized by 1H-NMR, differential scanning calorimetry (DSC), and powder X-ray diffraction (XRD) analyses. DSC and XRD results for PLA/PPAd blends (prepared by the solvent evaporation method) showed that these are immiscible, while enzymatic hydrolysis studies performed at 37 °C showed increased mass loss for PPAd compared to PLA...
August 13, 2018: Pharmaceutics
https://www.readbyqxmd.com/read/30103516/hydrogen-sulfide-releasing-fibrous-membranes-potential-patches-for-stimulating-human-stem-cells-proliferation-and-viability-under-oxidative-stress
#7
Ilaria Cacciotti, Matteo Ciocci, Emilia Di Giovanni, Francesca Nanni, Sonia Melino
The design of biomaterial platforms able to release bioactive molecules is mandatory in tissue repair and regenerative medicine. In this context, electrospinning is a user-friendly, versatile and low-cost technique, able to process different kinds of materials in micro- and nano-fibers with a large surface area-to-volume ratio for an optimal release of gaseous signaling molecules. Recently, the antioxidant and anti-inflammatory properties of the endogenous gasotramsmitter hydrogen sulfide (H₂S), as well as its ability to stimulate relevant biochemical processes on the growth of mesenchymal stem cells (MSC), have been investigated...
August 11, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30102527/biotin-modified-plga-nanoparticles-with-improved-antiproliferative-activity-of-15-16-dihydrotanshinone-i-in-human-cervical-cancer-cells
#8
Jingjing Luo, Xiaofeng Meng, Jianyu Su, Hang Ma, Wen Wang, Liming Fang, Huade Zheng, Yexia Qin, Tianfeng Chen
15,16-dihydrotanshinone I (DI), a natural compound isolated from a traditional Asian functional food Salvia Miltiorrhiza Bunge, is known for its anticancer activity. However, the poor solubility of DI limits its desirable anticancer application. Herein, poly lactic-co-glycolic acid (PLGA) was functionalized with polyethylene glycol (PEG) and biotin to form co-polymers PEG-PLGA (PPA) and biotin-PEG-PLGA (BPA). DI was encapsulated in co-polymers PPA and BPA to obtain DI-PPA-NPs and DI-BPA-NPs, respectively. The particle size and its distribution, encapsulation efficiency, and in vitro releasing capacity of DI-BPA-NPs were characterized by biophysical methods...
August 13, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/30102023/thermogel-loaded-with-low-dose-paclitaxel-as-a-facile-coating-to-alleviate-periprosthetic-fibrous-capsule-formation
#9
Jiabin Luan, Zheng Zhang, Wenjia Shen, Yipei Chen, Xiaowei Yang, Xiaobin Chen, Lin Yu, Jian Sun, Jiandong Ding
Medical-grade silicones as implants have been utilized for decades. However, the post-operative complications, such as capsular formation and contracture have not yet been fully controlled and resolved. The aim of the present study is to elucidate whether the capsular formation can be alleviated by local and sustained delivery of low-dose paclitaxel (PTX) during the critical phase after the insertion of silicone implants. A biocompatible and thermogelling poly(lactic acid- co-glycolic acid)- b-poly(ethylene glycol)- b-poly(lactic acid- co-glycolic acid) (PLGA-PEG-PLGA) triblock copolymer was synthesized by us...
August 13, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/30101836/clinical-significance-of-respiratory-compensation-during-exercise-testing-in-cardiac-patients
#10
Rujie Qin, Akira Koike, Osamu Nagayama, Yuta Takayanagi, Longmei Wu, Isao Nishi, Yuko Kato, Akira Sato, Takeshi Yamashita, Kazutaka Aonuma, Masaki Ieda
Ventilation (VE) increases linearly with the increase of carbon dioxide output (VCO2) during cardiopulmonary exercise testing. VE-VCO2 slope rises in parallel with exercise intensity, reaches a turning point (called the RC point), then steepens because of respiratory compensation for lactic acidosis. While this RC point can be identified universally, it is undetectable in some patients. In this study we evaluated whether the respiratory compensation during exercise testing has clinical significance in cardiac patients...
August 12, 2018: Bioscience Trends
https://www.readbyqxmd.com/read/30100498/therapeutic-effects-of-kefir-grain-lactobacillus-derived-extracellular-vesicles-in-mice-with-2-4-6-trinitrobenzene-sulfonic-acid-induced-inflammatory-bowel-disease
#11
M K Seo, E J Park, S Y Ko, E W Choi, S Kim
Kefir is a fermented product from yeast and lactic acid bacteria, and has been associated with various health benefits including relieving inflammatory bowel disease. Recently, it has been shown that gram-positive bacteria produce extracellular vesicles (EV). The EV could be appearing as potentially important mediators of cell to cell interaction. In this study, we explored the role of kefir grain Lactobacillus-derived EV in modulating inflammation responses via alleviating the production of inflammatory cytokines in tumor necrosis factor-α (TNF-α)-induced inflammation in Caco-2 cells and the 2,4,6-trinitrobenzene sulfonic acid-induced inflammatory bowel disease mouse model...
August 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/30099846/glar-yuga-a-novel-rpir-family-transcription-activator-of-the-leloir-pathway-of-galactose-utilization-in-lactococcus-lactis-il1403
#12
Tamara Aleksandrzak-Piekarczyk, Katarzyna Szatraj, Katarzyna Kosiorek
Bacteria can utilize diverse sugars as carbon and energy source, but the regulatory mechanisms directing the choice of the preferred substrate are often poorly understood. Here, we analyzed the role of the YugA protein (now designated GlaR-Galactose-lactose operon Regulatory protein) of the RpiR family as a transcriptional activator of galactose (gal genes) and lactose (lac genes) utilization genes in Lactococcus lactis IL1403. In this bacterium, gal genes forming the Leloir operon are combined with lac genes in a single so-called gal-lac operon...
August 11, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/30099702/electrical-weapons-and-excited-delirium-shocks-stress-and-serum-serotonin
#13
Mark W Kroll, Stacey L Hail, Ryan M Kroll, Charles V Wetli, John C Criscione
It has been suggested that a CEW (conducted electrical weapon) exposure could elicit a stress response that could cause ExDS (excited delirium syndrome). There are some parallels between the signs of ExDS and serotonin syndrome (SS). Electroconvulsive therapy raises serotonin levels and therefore provides a plausible link between CEW applications and elevated serotonin levels. This study was designed to determine whether a CEW exposure elevates serum serotonin. A total of 31 police academy cadets were exposed to a very broad-spread 5-s CEW stimulus from a TASER brand X26 CEW...
August 11, 2018: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/30098415/is-seashell-powder-suitable-for-phosphate-recovery-from-fermentation-broth
#14
Jan Christoph Peinemann, Lina Maja Maria Krenz, Daniel Pleissner
This communication elaborates on the use of seashell powder (SP) for the removal of phosphate from lactic acid-containing fermentation broth. Despite extensive past research regarding the application of SP for phosphate removal from wastewater, no information is available for solutions containing various organic compounds. In order to fill this knowledge gap, tests were performed with pure phosphate solution (PPS) and PPS containing 0.83 M of three alcohols, ethanol, propanol or 1,2-propanediol, or 0.83 M of three organic acids, acetic, propionic or lactic acid...
August 8, 2018: New Biotechnology
https://www.readbyqxmd.com/read/30098254/-analysis-of-a-female-neonate-with-pyruvate-dehydrogenase-complex-deficiency
#15
Kaihui Zhang, Hongying Li, Xiaoying Li, Yi Liu, Zhongtao Gai
OBJECTIVE: To analyze the clinical features and genetic basis of a female neonate with muscle weakness, abnormal brain magnetic resonance imaging and elevated blood lactate. METHODS: The patient was subjected to clinical and laboratory examination. Next generation sequencing was carried out for the patient and her relatives. RESULTS: The proband was diagnosed as small for gestational age, with clinical features including muscle weakness, abnormal brain magnetic resonance imaging, increased blood lactate, and acidosis...
August 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/30096995/six-month-evaluation-of-novel-bioabsorbable-scaffolds-composed-of-poly-l-lactic-acid-and-amorphous-calcium-phosphate-nanoparticles-in-porcine-coronary-arteries
#16
Thanh Dinh Nguyen, Gaoke Feng, Xin Yi, Yongnan Lyu, Zhiyuan Lan, Jinggang Xia, Tim Wu, Xuejun Jiang
Objective Using coronary angiography and intravascular ultrasound methods to evaluate the performance of the novel fully bioabsorbable scaffold (NFBS) composed of poly-L-lactic acid/amorphous calcium phosphate (PLLA/ACP) at six-month follow-up by comparing with PLLA scaffolds Methods Twelve PLLA/ACP scaffolds and 12 PLLA scaffolds were implanted into the coronary arteries of 12 miniature pigs. Quantitative coronary angiography (QCA) was used to measure the reference vessel diameter (RVD), mean lumen diameter (MLD) and late lumen loss (LLL)...
August 2018: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/30096901/characterization-of-antibacterial-cell-free-supernatant-from-oral-care-probiotic-weissella-cibaria-cmu
#17
Hae-Soon Lim, Ji-Eun Yeu, Sang-Phil Hong, Mi-Sun Kang
Recently, studies have explored the use of probiotics like the Weissella cibaria strain, CMU (oraCMU), for use as preventive dental medicine instead of chemical oral care methods. The present study was conducted to investigate the antibacterial properties of the cell-free supernatant (CFS) from this bacterium. Cell morphology using the scanning electron microscope, and the antibacterial effect of CFS under various growth conditions were evaluated. The production of hydrogen peroxide, organic acids, fatty acids, and secretory proteins was also studied...
August 9, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30096797/effect-of-milk-fermented-with-lactobacillus-fermentum-on-the-inflammatory-response-in-mice
#18
Lourdes Santiago-López, Adrián Hernández-Mendoza, Verónica Mata-Haro, Belinda Vallejo-Córdoba, Abraham Wall-Medrano, Humberto Astiazarán-García, María Del Carmen Estrada-Montoya, Aarón F González-Córdova
Currently, the effect of fermented milk on the T-helper 17 response in inflammatory bowel diseases (IBDs) is unknown. The aim of the present study was to evaluate the effect of milks fermented with Lactobacillus fermentum on the Th1/Th17 response in a murine model of mild IBD. Exopolysaccharide (EPS), lactic acid (LA), and total protein (TP) contents and bacterial concentration were determined. Male C57Bl/6 mice intragastrically received either raw (FM) or pasteurized (PFM) fermented milk before and during a dextran sulfate infusion protocol...
August 8, 2018: Nutrients
https://www.readbyqxmd.com/read/30096155/interactions-of-organic-acids-with-campylobacter-coli-from-swine
#19
Ross C Beier, Roger B Harvey, Charles A Hernandez, Michael E Hume, Kathleen Andrews, Robert E Droleskey, Maureen K Davidson, Sonia Bodeis-Jones, Shenia Young, Sara E Duke, Robin C Anderson, Tawni L Crippen, Toni L Poole, David J Nisbet
Campylobacter coli is a bacterial species that is a major cause of diarrheal disease worldwide, and Campylobacter spp. are among the top 5 foodborne pathogens in the United States. During food production organic acids (OAs) are often used to remove bacteria from animal carcasses. The interactions of six OAs with 111 C. coli strains obtained from swine and retail pork chops were studied by determining the molar minimum inhibitory concentrations (MICMs) of the C. coli strains, and the pH at the MICMs. The Henderson-Hasselbalch equation was used to calculate the concentrations of the undissociated and dissociated OAs at the MICMs of the C...
2018: PloS One
https://www.readbyqxmd.com/read/30094534/fragment-of-the-aspartyl-trna-synthetase-applicable-as-a-shared-classification-and-phylogenetic-marker-in-particular-representatives-of-the-order-lactobacillales
#20
Chahrazed Mekadim, Jiří Killer, Radko Pechar, Jakub Mrázek
The order Lactobacillales represents a morphologically, metabolically, and physiologically diverse group of bacteria. Lactic acid bacteria represent the core of this phylogenetic group. They are a part of epiphytic microflora, fermented dairy, meat, fruit and vegetable products, and the digestive tract of humans and animals. Despite the fact that these bacteria form a phenotypically and genotypically heterogeneous group, their phylogenetic relationship enables to propose a common genetic marker usable in classification, typing, and phylogeny...
August 9, 2018: Folia Microbiologica
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