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https://www.readbyqxmd.com/read/30547194/dynamics-of-biofilm-formation-by-salmonella-typhimurium-and-beef-processing-plant-bacteria-in-mono-and-dual-species-cultures
#1
Jeyachchandran Visvalingam, Peipei Zhang, Timothy C Ells, Xianqin Yang
This study aimed to determine the impact of bacteria from a beef plant conveyor belt on the biofilm formation of Salmonella in dual-species cultures. Beef plant isolates (50) including 18 Gram-negative aerobes (GNA), 8 Gram-positive aerobes (GPA), 5 lactic acid bacteria (LAB), 9 Enterobacteriaceae (EB), and 10 generic Escherichia coli (GEC) were included for developing biofilms in mono- and co-culture with S. Typhimurium at 15 °C for 6 days. Five selected cultures in planktonic form and in biofilms were tested for susceptibility to two commonly used sanitizers (i...
December 13, 2018: Microbial Ecology
https://www.readbyqxmd.com/read/30545357/mitochondrial-gene-sequence-variants-in-children-with-severe-malaria-anaemia-with-or-without-lactic-acidosis-a-case-control-study
#2
Casey Fowler, Christine Cserti-Gazdewich, Aggrey Dhabangi, Charles Musoke, Himanshu Sharma, Sami S Amr, Walter Dzik
BACKGROUND: Evolutionary pressure by Plasmodium falciparum malaria is known to have favoured a large number of human gene adaptations, but there is surprisingly little investigation of the effect of malaria on human mitochondrial sequence variation. Plasmodium falciparum infection can cause severe malaria anaemia (SMA) with insufficient tissue oxygenation, lactic acidosis and death. Despite equal degrees of severe anaemia, some individuals develop lactic acidosis while others do not. A case-control study design was used to investigate whether differences in host mitochondrial gene sequences were associated with lactic acidosis in SMA...
December 13, 2018: Malaria Journal
https://www.readbyqxmd.com/read/30545116/optimization-of-thermoplastic-blend-matrix-hdpe-pla-with-different-types-and-levels-of-coupling-agents
#3
Alessia Quitadamo, Valérie Massardier, Carlo Santulli, Marco Valente
High-density polyethylene (HDPE) and poly(lactic) acid (PLA) blends with different ratios of both polymers, namely, 30:70, 50:50, and 70:30, were produced. Polyethylene-grafted maleic anhydride and a random copolymer of ethylene and glycidyl methacrylate were also considered as compatibilizers to modify HDPE/PLA optimal blends and were added in the amounts of 1, 3, and 5 wt.%. Different properties of the blends were evaluated by performing tensile tests and scanning electron microscopy to analyze blend and interfaces morphology...
December 12, 2018: Materials
https://www.readbyqxmd.com/read/30544799/iron-supplements-containing-lactobacillus-plantarum-299v-increase-ferric-iron-and-up-regulate-the-ferric-reductase-dcytb-in-human-caco-2-ht29-mtx-co-cultures
#4
Ann-Sofie Sandberg, Gunilla Önning, Niklas Engström, Nathalie Scheers
Several human interventions have indicated that Lactobacillus plantarum 299v ( L. plantarum 299v) increases intestinal iron absorption. The aim of the present study was to investigate possible effects of L. plantarum 299v on the mechanisms of iron absorption on the cellular level. We have previously shown that lactic fermentation of vegetables increased iron absorption in humans. It was revealed that the level of ferric iron [Fe (H₂O)₅]2+ was increased after fermentation. Therefore, we used voltammetry to measure the oxidation state of iron in simulated gastrointestinal digested oat and mango drinks and capsule meals containing L...
December 8, 2018: Nutrients
https://www.readbyqxmd.com/read/30544776/the-effect-of-organic-acid-trisodium-phosphate-and-essential-oil-component-immersion-treatments-on-the-microbiology-of-cod-gadus-morhua-during-chilled-storage
#5
Conor Smyth, Nigel P Brunton, Colin Fogarty, Declan J Bolton
Spoilage is a major issue for the seafood sector with the sale and exportation of fish limited by their short shelf-life. The immediate and storage effects of immersion (30 s at 20 °C) with 5% ( w / v ) citric acid (CA), 5% ( v / v ) lactic acid (LA), 5% ( w / v ) capric acid (CP) and 12% trisodium phosphate (TSP) (experiment 1) and essential oil components (EOC) (1% ( v / v ) citral (CIT), 1% ( v / v ) carvacrol (CAR), 1% ( w / v ) thymol (THY) and 1% ( v / v ) eugenol (EUG)) (experiment 2) on the concentrations of indicator (total viable counts (TVC) (mesophilic and psychrophilic) and total Enterobacteriaceae counts (TEC)), and spoilage organisms ( Pseudomonas spp...
December 8, 2018: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/30544548/deep-eutectic-solvent-based-ultrahigh-pressure-extraction-of-baicalin-from-scutellaria-baicalensis-georgi
#6
Hui Wang, Xiaodi Ma, Qibin Cheng, Li Wang, Liwei Zhang
Deep eutectic solvents (DESs), promising green solvents, and ultrahigh pressure extraction (UPE) as an effective auxiliary extraction method, have attracted wide attention. In this study, DES was coupled with UPE to efficiently extract baicalin from Scutellaria baicalensis Georgi. First, choline chloride: lactic acid (ChCl-LA, molar ratio 1:1) was selected as the most appropriate DES by comparing the extraction yield of different DESs. Second, the extraction protocol was optimized by response surface methodology (RSM) considering the impacts of ChCl-LA concentration, extraction pressure, extraction time and liquid-solid ratio on the extraction yield...
December 7, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30543890/composite-scaffold-obtained-by-electro-hydrodynamic-technique-for-infection-prevention-and-treatment-in-bone-repair
#7
Javier Aragón, Sergio Feoli, Silvia Irusta, Gracia Mendoza
Bone infection is a devastating condition resulting from implant or orthopaedic surgery. Therapeutic strategies are extremely complicated and may result in serious side effects or disabilities. The development of enhanced 3D scaffolds, able to promote efficient bone regeneration, combined with targeted antibiotic release to prevent bacterial colonization, is a promising tool for the successful repair of bone defects. Herein, polymeric electrospun scaffolds composed of polycaprolactone (PCL) nanofibres decorated with poly(lactic-co-glycolic acid) (PLGA) particles loaded with rifampicin were fabricated to achieve local and sustained drug release for more efficient prevention and treatment of infection...
December 10, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/30543778/microbiota-derived-lactate-accelerates-intestinal-stem-cell-mediated-epithelial-development
#8
Yong-Soo Lee, Tae-Young Kim, Yeji Kim, Su-Hyun Lee, Seungil Kim, Sung Wan Kang, Jin-Young Yang, In-Jeoung Baek, Young Hoon Sung, Yun-Yong Park, Sung Wook Hwang, Eunju O, Kwang Soon Kim, Siqing Liu, Nobuhiko Kamada, Nan Gao, Mi-Na Kweon
Symbionts play an indispensable role in gut homeostasis, but underlying mechanisms remain elusive. To clarify the role of lactic-acid-producing bacteria (LAB) on intestinal stem-cell (ISC)-mediated epithelial development, we fed mice with LAB-type symbionts such as Bifidobacterium and Lactobacillus spp. Here we show that administration of LAB-type symbionts significantly increased expansion of ISCs, Paneth cells, and goblet cells. Lactate stimulated ISC proliferation through Wnt/β-catenin signals of Paneth cells and intestinal stromal cells...
December 12, 2018: Cell Host & Microbe
https://www.readbyqxmd.com/read/30543484/noninvasive-biodegradable-nanoparticles-in-nanofibers-single-dose-ocular-insert-in-vitro-ex-vivo-and-in-vivo-evaluation
#9
Islam A Khalil, Isra H Ali, Ibrahim M El-Sherbiny
AIM: This study involves, for the first time, the development of mucoadhesive biodegradable polymeric-multilayered nanoparticles-in-nanofibers (NPs-in-NFs) matrix as an innovative single-dose noninvasive ocular-insert that could substitute conventional ocular dosage-forms. MATERIALS & METHODS: Azithromycin-loaded poly(lactic-co-glycolic acid) copolymer/pluronic NPs were developed then incorporated into electrospun polyvinylpyrrolidone NFs, and tested for their efficient treatment of ocular bacterial infection...
December 13, 2018: Nanomedicine
https://www.readbyqxmd.com/read/30542415/targeting-lung-cancer-initiating-cells-by-all-trans-retinoic-acid-loaded-lipid-plga-nanoparticles-with-cd133-aptamers
#10
Yu Zhang, Juan Zhao, Jing Sun, Lu Huang, Qingfeng Li
Lung cancer initiating cells represent a specific subpopulation of lung cancer cells, which significantly contribute to the initiation, metastasis and recurrence of lung cancer. CD133, initially considered a marker of stem cells, is now considered as a marker for lung cancer initiating cells. All-trans retinoic acid (RA) has been demonstrated to cause the differentiation, inhibition of proliferation, and apoptosis of cancer cells and cancer initiating cells. However, there have been no reports on the activity of RA against lung cancer initiating cells...
December 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30541701/nanofiber-arrangement-regulates-peripheral-nerve-regeneration-through-differential-modulation-of-macrophage-phenotypes
#11
Yachao Jia, Weichao Yang, Kuihua Zhang, Shuo Qiu, Jia Xu, Chunyang Wang, Yimin Chai
Topographical cues presented by aligned nanofibers have been demonstrated to stimulate peripheral nerve regeneration across long gaps, but the underlying mechanisms remain incompletely elucidated. Because macrophages play a crucial role in peripheral nerve regeneration and can be phenotypically modulated by topographical cues, we hypothesized that aligned nanofibers might induce the development of macrophage phenotypes that facilitate the regeneration of peripheral nerves. Here, macrophages were seeded on aligned and random poly(l-lactic acid-co-ε-caprolactone) nanofibers and their morphology and phenotypes were compared...
January 1, 2019: Acta Biomaterialia
https://www.readbyqxmd.com/read/30541659/-hyperlactatemia-induced-by-inhaled-%C3%AE-2-agonists-an-underrecognized-side-effect-report-of-two-cases
#12
C David, J Pouchot, B Ranque
BACKGROUND: Repeated use of inhaled β2 agonists is common in asthma or COPD exacerbations. It can lead to hyperlactatemia. CASE REPORTS: We report two asthmatic patients who presented in the emergency department for an asthma exacerbation. The first patient developed hyperlactatemia at 3.9 mmol/L and the second patient developed hyperlactatemia at 5.6 mmol/L after terbutaline treatment. Both patients had a favorable outcome after adjusting the aerosol dose to clinical parameters...
December 9, 2018: La Revue de Médecine Interne
https://www.readbyqxmd.com/read/30540795/physicochemical-and-microbiological-characterization-of-the-sensory-deviation-responsible-for-the-origin-of-the-special-sherry-wines-palo-cortado-type
#13
Victor Palacios, Ana Roldán, Ana Jiménez-Cantizano, Antonio Amores-Arrocha
The aim of this study was to characterize the biochemical changes and microbiological processes involved in the sensory deviation of "sobretablas" wines during biological aging, which leads to the origin of special or rare "palo cortado" wines. Industrial trials of biological aging of "sobretablas" wines with the potential for the development of lactic acid bacteria (LAB) were performed to study this phenomenon. The results showed that sensory deviation was due to the development of malolactic fermentation (MLF) together with an attenuated aerobic metabolism of "flor" yeast...
2018: PloS One
https://www.readbyqxmd.com/read/30539000/heteroplasmy-detection-of-mitochondrial-dna-a3243g-mutation-using-quantitative-real-time-pcr-assay-based-on-taqman-mgb-probes
#14
Enguang Rong, Hanbo Wang, Shujing Hao, Yuhong Fu, Yanyan Ma, Tianze Wang
A point mutation of mitochondrial DNA (mtDNA) at nucleotide position 3243 A to G (mt.3243A>G) is involved in many common diseases, including maternally inherited diabetes and deafness (MIDD) and mitochondrial encephalomyopathy, lactic acidosis with stroke-like episodes (MELAS). However, the mutant level of mt.3243A>G varies both among individuals and in different organs, tissues, and even cells of single individuals. For detection of this mutation, current methods have limited universality and sensitivity and may be not adequate for a routine clinical test...
2018: BioMed Research International
https://www.readbyqxmd.com/read/30538693/occurrence-and-dynamism-of-lactic-acid-bacteria-in-distinct-ecological-niches-a-multifaceted-functional-health-perspective
#15
REVIEW
Fanny George, Catherine Daniel, Muriel Thomas, Elisabeth Singer, Axel Guilbaud, Frédéric J Tessier, Anne-Marie Revol-Junelles, Frédéric Borges, Benoît Foligné
Lactic acid bacteria (LAB) are representative members of multiple ecosystems on earth, displaying dynamic interactions within animal and plant kingdoms in respect with other microbes. This highly heterogeneous phylogenetic group has coevolved with plants, invertebrates, and vertebrates, establishing either mutualism, symbiosis, commensalism, or even parasitism-like behavior with their hosts. Depending on their location and environment conditions, LAB can be dominant or sometimes in minority within ecosystems...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30538550/erratum-prognostic-impact-of-lactic-dehydrogenase-to-albumin-ratio-in-hepatocellular-carcinoma-patients-with-child-pugh-i-who-underwent-curative-resection-a-prognostic-nomogram-study-erratum
#16
(no author information available yet)
[This corrects the article on p. 5383 in vol. 10, PMID: 30464634.].
2018: Cancer Management and Research
https://www.readbyqxmd.com/read/30538075/the-influence-of-telmisartan-on-metformin-pharmacokinetics-and-pharmacodynamics
#17
Jiagen Wen, Meizi Zeng, Zhaoqian Liu, Honghao Zhou, Heng Xu, Min Huang, Wei Zhang
Metformin is the most widely used drug among type 2 diabetes mellitus patients. However, drug interaction on metformin will influence its glucose-lowering effect or increase its side effect of lactic acidosis. In this study, a randomized, two-stage, crossover study was conducted to unveil the potential drug interaction between metformin and the anti-hypertension drug, telmisartan. Totally, 16 healthy Chinese male volunteers were enrolled. Blood samples from various time-points after drug adminstration were analyzed for metformin quantification...
November 25, 2018: Journal of Pharmacological Sciences
https://www.readbyqxmd.com/read/30537110/combination-of-whole-grape-seed-flour-and-newly-isolated-kefir-lactic-acid-bacteria-reduces-high-fat-hf-induced-hepatic-steatosis
#18
Ji-Hye Kwon, Hyeon Gyu Lee, Kun-Ho Seo, Hyunsook Kim
SCOPE: This study aimed to determine the separate and combined effect of a prebiotic (Catechin-rich wine grape seed flour, GSF) and a probiotic (newly isolated kefir lactic acid bacteria, LAB) on hepatic steatosis of obese mice. METHODS AND RESULTS: High-fat (HF)-induced obese mice were fed HF diet containing 6% microcrystalline cellulose (MCC, control), 10% GSF, orally administrated LAB, or combination of GSF and LAB for 9 weeks. There was a significant reduction of body weight gain, and liver weights, plasma insulin concentrations, and HOMA-IR in all experimental groups compared to control...
December 11, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/30535454/cd38-affects-the-biological-behavior-and-energy-metabolism-of-nasopharyngeal-carcinoma-cells
#19
Yanshan Ge, Yuehua Long, Songshu Xiao, Lin Liang, Zhengxi He, Chunxue Yue, Xiong Wei, Yanhong Zhou
Nasopharyngeal carcinoma (NPC) is the most common malignant tumor type in Southern China and South‑East Asia. Cluster of differentiation (CD)38 is highly expressed in the human immune system and participates in the activation of T, natural killer and plasma cells mediated by CD2 and CD3 through synergistic action. CD38 is a type II transmembrane glycoprotein, which was observed to mediate diverse activities, including signal transduction, cell adhesion and cyclic ADP‑ribose synthesis. However, the significance of CD38 in NPC biological behavior and cellular energy metabolism has not been examined...
December 3, 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/30535308/cardiomyopathy-associated-with-mitochondrial-encephalopathy-lactic-acidosis-and-stroke-like-episodes-melas-syndrome
#20
Soo-Kyeong Song, Sun Hwa Lee
No abstract text is available yet for this article.
December 7, 2018: QJM: Monthly Journal of the Association of Physicians
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