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Tissue Engineered

Ying Wang, Shukui Zhou, Ranxing Yang, Qingsong Zou, Kaile Zhang, Qinghua Tian, Weixin Zhao, Lijuan Zong, Qiang Fu
Cell-seeded scaffolds are a common route of cell transplantation for bladder repair and reconstruction. However, when cell suspensions are harvested, proteolytic enzymes often cause extracellular matrix damage and loss of intercellular junctions. To overcome this problem, we developed a bioengineered three-dimensional bladder patch comprising porous scaffolds and multilayered adipose-derived stem cell (ASC) sheets, and evaluated its feasibility for bladder regeneration in a rat model. Adipose-derived stem cells (ASCs) were labeled with ultrasmall super-paramagnetic iron oxide (USPIO) nanoparticles...
December 12, 2018: Acta Biomaterialia
Patricia Diaz-Rodriguez, Mirian Sánchez, Mariana Landin
The mimesis of biological systems has been demonstrated to be an adequate approach to obtain tissue engineering scaffolds able to promote cell attachment, proliferation, and differentiation abilities similar to those of autologous tissues. Bioceramics are commonly used for this purpose due to their similarities to the mineral component of hard tissues as bone. Furthermore, biomimetic scaffolds are frequently loaded with diverse therapeutic molecules to enhance their biological performance, leading to final products with advanced functionalities...
December 13, 2018: Pharmaceutics
Gianrico Spagnuolo, Bruna Codispoti, Massimo Marrelli, Carlo Rengo, Sandro Rengo, Marco Tatullo
Tissue engineering is based on the interaction between stem cells, biomaterials and factors delivered in biological niches. Oral tissues have been found to be rich in stem cells from different sources: Stem cells from oral cavity are easily harvestable and have shown a great plasticity towards the main lineages, specifically towards bone tissues. Dental pulp stem cells (DPSCs) are the most investigated mesenchymal stem cells (MSCs) from dental tissues, however, the oral cavity hosts several other stem cell lineages that have also been reported to be a good alternative in bone tissue engineering...
December 13, 2018: Dentistry journal
Samira Jafari, Hossein Derakhshankhah, Loghman Alaei, Ali Fattahi, Behrang Shiri Varnamkhasti, Ali Akbar Saboury
Based on unique intrinsic properties of mesoporous silica nanoparticles (MSNs) such as high surface area, large pore size, good biocompatibility and biodegradability, stable aqueous dispersion, they have received much attention in the recent decades for their applications as a promising platform in the biomedicine field. These porous structures possess a pore size ranging from 2 to 50 nm which make them excellent candidates for various biomedical applications. Herein, at first we described the common approaches of cargo loading and release processes from MSNs...
January 2019: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Panittha Damsongsang, Daneeya Chaikiawkeaw, Waranyoo Phoolcharoen, Kaewta Rattanapisit, Pornjira Kaewpungsup, Prasit Pavasant, Voravee P Hoven
In this report, recombinant human osteopontin synthesized in tobacco plants (p-rhOPN) is introduced as a potential bioactive molecule that can promote osteoblast adhesion and differentiation. A glass substrate (SiO2 /Si-OH) grafted with poly(acrylic acid) (SiO2 /Si-PAA) was prepared by surface-initiated reversible addition-fragmentation chain transfer polymerization and used as a carboxyl-rich platform for the chemical conjugation of p-rhOPN. The PAA grafting and subsequent p-rhOPN immobilization were confirmed by water contact angle, Fourier transform-infrared spectroscopy, X-ray photoelectron spectroscopy and atomic force microscopy analyses...
January 1, 2019: Colloids and Surfaces. B, Biointerfaces
Valeria Graceffa, Dimitrios I Zeugolis
Chondrocyte-based tissue engineering requires in vitro cell expansion, which is associated with phenotypic losses, decrease in collagen II synthesis and increase in collagen I synthesis. Another major obstacle in clinical translation of chondrocyte-based therapies is the lack of extracellular matrix (ECM) in the engineered cartilage substitutes. Various research and commercially available media claim that can maintain chondrogenic phenotype, whilst macromolecular crowding (MMC) has been shown to increase tissue-specific ECM deposition and to maintain cell phenotype in vitro...
December 14, 2018: Journal of Tissue Engineering and Regenerative Medicine
Klara Janjić, Peter Bauer, Michael Edelmayer, Barbara Cvikl, Barbara Schädl, Andreas Moritz, Hermann Agis
BACKGROUND: A major mediator of angiogenesis is angiogenin which is expressed in the early phase of healing in oral tissue engineering strategies. It is unclear how angiogenin is regulated in the periodontal tissue. The objective of this study was to reveal the regulation of angiogenin in response to hypoxia and the hypoxia mimetic agent L-mimosine in periodontal fibroblasts METHODS: Human fibroblasts of the periodontal ligament (PDLF) and the gingiva (GF) in monolayer and spheroid cultures were exposed to hypoxia or L-mimosine...
December 14, 2018: Journal of Periodontology
Ke Huang, Shiqi Hu, Ke Cheng
Myocardial infarction (MI), caused by coronary heart disease (CHD), remains one of the most common causes of death in the United States. Over the last few decades, scientists have invested considerable resources on the study and development of cell therapies for myocardial regeneration after MI. However, due to a number of limitations, they are not yet readily available for clinical applications. Mounting evidence supports the theory that paracrine products are the main contributors to the regenerative effects attributed to these cell therapies...
December 13, 2018: Advanced Healthcare Materials
Sarah A Richardson, Thomas M Rawlings, Joanne Muter, Marc Walker, Jan J Brosens, Neil R Cameron, Ahmed M Eissa
A novel strategy for the surface functionalization of emulsion-templated highly porous (polyHIPE) materials as well as its application to in vitro 3D cell culture is presented. A heterobifunctional linker that consists of an amine-reactive N-hydroxysuccinimide ester and a photoactivatable nitrophenyl azide, N-sulfosuccinimidyl-6-(4'-azido-2'-nitrophenylamino)hexanoate (sulfo-SANPAH), is utilized to functionalize polyHIPE surfaces. The ability to conjugate a range of compounds (6-aminofluorescein, heptafluorobutylamine, poly(ethylene glycol) bis-amine, and fibronectin) to the polyHIPE surface is demonstrated using fluorescence imaging, FTIR spectroscopy, and X-ray photoelectron spectroscopy...
December 13, 2018: Macromolecular Bioscience
Ehsan Saburi, Hadi Atabati, Ladan Kabiri, Asma Behdari, Mina Azizi, Abdolreza Ardeshirylajimi, Seyed Ehsan Enderami, Sayyed Mohammad-Hossein Ghaderian, Mohsen Nafar, Mahmoud Parvin, Mir Davood Omrani
Renal failures treatment has been faced with several problems during the last decades. Kidney tissue engineering has been created many hopes to improve treatment procedures with scaffold fabrication that can modulate kidney cells/stem cells migration to the lesion site and increase the survival of these cells at that site with imitating the role of the kidney extracellular matrix. In this study, bone morphogenetic protein-7 (BMP7) as a vital factor for kidney development and regeneration was incorporated in the polycaprolactone (PCL) nanofibers and after morphological, mechanical, and biocompatible characterization, proliferation, and survival of the human embryonic kidney cells (HEK) were investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), flow cytometry, and gene expression while cultured on scaffolds...
December 12, 2018: Journal of Cellular Biochemistry
Sarah Brookes, Sherry Voytik-Harbin, Hongji Zhang, Lujuan Zhang, Stacey Halum
OBJECTIVE: Tissue engineering of the larynx requires a complex, multiple tissue layer design. Additionally, spontaneous reinnervation of the larynx after recurrent laryngeal nerve (RLN) injury is often disorganized, resulting in subpar function. This study investigates use of tissue-engineered cartilage and motor endplate-expressing (MEE) tissue-engineered skeletal muscle implants for laryngeal reconstruction and the promotion of organized reinnervation after RLN injury. METHODS: F344 rat primary muscle progenitor cells (MPCs) were isolated...
December 12, 2018: Laryngoscope
Hoon Seonwoo, Beomyong Shin, Kyoung-Je Jang, Myungchul Lee, Oak-Sung Choo, Sang-Bae Park, Yeong Cheol Kim, Mi-Jin Choi, Jangho Kim, Pankaj Garg, Jeong Hun Jang, Yun-Hoon Choung, Jong Hoon Chung
Chronic tympanic membrane (TM) perforations can cause otorrhea. To date, various types of tissue engineering techniques have been applied for the regeneration of chronic TM perforations. However, the application of nanofibers with radially aligned nanostructures and the simultaneous release of growth factors have never been applied in the regeneration of chronic TM perforations. Here, epidermal growth factor (EGF)-releasing radially aligned nanofibrous patches (ERA-NFPs) are developed and applied for the regeneration of chronic perforated TMs...
December 12, 2018: Advanced Healthcare Materials
Mohammad Ojaghi, Fatemeh Soleimanifar, Alireza Kazemi, Marzieh Ghollasi, Masoud Soleimani, Nikoo Nasoohi, Seyed Ehsan Enderami
Combination of adipose-derived mesenchymal stem cells (ADSCs) and synthetic materials in terms of pancreatic tissue engineering can be considered as a treatment of diabetes. This study aimed to evaluate the differentiation of human ADSCs to pancreatic cells on poly-l-lactic acid/polyvinyl alcohol (PLLA/PVA) nanofibers as a three-dimensional (3D) scaffold. Mesenchymal stem cells (MSCs) were characterized for mesenchymal surface markers by flow cytometry. Then ADSCs were seeded on 3D scaffolds and treated with pancreatic differentiation medium...
December 11, 2018: Journal of Cellular Biochemistry
Shigeru Miyagawa, Keitaro Domae, Satoshi Kainuma, Ryouhei Matsuura, Daisuke Yoshioka, Hiroki Hata, Yasushi Yoshikawa, Koichi Toda, Yoshiki Sawa
BACKGROUND: Dilated cardiomyopathy (DCM) is a life-threatening heart muscle disease characterized by progressive heart failure, which often requires left ventricular assist device (LVAD) implantation or heart transplantation (HTx). A tissue engineering strategy using cell sheets for end-stage heart failure has emerged in recent years. CASE PRESENTATION: Here, we describe a 50-year-old DCM patient with severe symptoms of heart failure with severe mitral regurgitation (MR) who was not a candidate for LVAD or HTx...
December 13, 2018: Surgical Case Reports
Sam L Francis, Claudia Di Bella, Gordon G Wallace, Peter F M Choong
There is no long-term treatment strategy for young and active patients with cartilage defects. Early and effective joint preserving treatments in these patients are crucial in preventing the development of osteoarthritis. Tissue engineering over the past few decades has presented hope in overcoming the issues involved with current treatment strategies. Novel advances in 3D bioprinting technology have promoted more focus on efficient delivery of engineered tissue constructs. There have been promising in-vitro studies and several animal studies looking at 3D bioprinting of engineered cartilage tissue...
2018: Frontiers in Surgery
Ian Holland, Jack Logan, Jiezhong Shi, Christopher McCormick, Dongsheng Liu, Wenmiao Shu
The therapeutic replacement of diseased tubular tissue is hindered by the availability and suitability of current donor, autologous and synthetically derived protheses. Artificially created, tissue engineered, constructs have the potential to alleviate these concerns with reduced autoimmune response, high anatomical accuracy, long-term patency and growth potential. The advent of 3D bioprinting technology has further supplemented the technological toolbox, opening up new biofabrication research opportunities and expanding the therapeutic potential of the field...
2018: Bio-design and manufacturing
Elisabeth L Gill, Xia Li, Mark A Birch, Yan Yan Shery Huang
It is envisaged that the creation of cellular environments at multiple length scales, that recapitulate in vivo bioactive and structural roles, may hold the key to creating functional, complex tissues in the laboratory. This review considers recent advances in biofabrication and bioprinting techniques across different length scales. Particular focus is placed on 3D printing of hydrogels and fabrication of biomaterial fibres that could extend the feature resolution and material functionality of soft tissue constructs...
2018: Bio-design and manufacturing
Hasan Erbil Abaci, Abigail Coffman, Yanne Doucet, James Chen, Joanna Jacków, Etienne Wang, Zongyou Guo, Jung U Shin, Colin A Jahoda, Angela M Christiano
Human skin constructs (HSCs) have the potential to provide an effective therapy for patients with significant skin injuries and to enable human-relevant drug screening for skin diseases; however, the incorporation of engineered skin appendages, such as hair follicles (HFs), into HSCs remains a major challenge. Here, we demonstrate a biomimetic approach for generation of human HFs within HSCs by recapitulating the physiological 3D organization of cells in the HF microenvironment using 3D-printed molds. Overexpression of Lef-1 in dermal papilla cells (DPC) restores the intact DPC transcriptional signature and significantly enhances the efficiency of HF differentiation in HSCs...
December 13, 2018: Nature Communications
Xianrui Yang, Li Li, Li Xiao, Donghui Zhang
Adult stem cells are excellent cell resource for cell therapy and regenerative medicine. Dental pulp stem cells (DPSCs) have been discovered and well known in various application. Here, we reviewed the history of dental pulp stem cell study and the detail experimental method including isolation, culture, cryopreservation, and the differentiation strategy to different cell lineage. Moreover, we discussed the future potential application of the combination of tissue engineering and of DPSC differentiation. This review will help the new learner to quickly get into the DPSC filed...
December 13, 2018: Stem Cell Research & Therapy
Le Guan, Muhammad Salman Haider, Nadeem Khan, Maazullah Nasim, Songtao Jiu, Muhammad Fiaz, Xudong Zhu, Kekun Zhang, Jinggui Fang
Salinity is ubiquitous abiotic stress factor limiting viticulture productivity worldwide. However, the grapevine is vulnerable to salt stress, which severely affects growth and development of the vine. Hence, it is crucial to delve into the salt resistance mechanism and screen out salt-resistance prediction marker genes; we implicated RNA-sequence (RNA-seq) technology to compare the grapevine transcriptome profile to salt stress. Results showed 2472 differentially-expressed genes (DEGs) in total in salt-responsive grapevine leaves, including 1067 up-regulated and 1405 down-regulated DEGs...
December 12, 2018: International Journal of Molecular Sciences
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