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Tissue Engineered

Francisco Martín-Saavedra, Clara Escudero-Duch, Martín Prieto, Silvia Sánchez-Casanova, Daniel López, Manuel Arruebo, Richard Voellmy, Jesús Santamaría, Nuria Vilaboa
CuS nanoparticles (CuSNP) are degradable, readily prepared, inexpensive to produce and efficiently cleared from the body. In this work, we explored the feasibility of CuSNP to function as degradable near infrared (NIR) nanotransducers within fibrin-based cellular scaffolds. To prepare NIR-responsive CuSNP hydrogels, fibrinogen was dissolved in cell culture medium and supplemented with aqueous dispersions of CuSNP. Fibrinogen polymerization was catalyzed by the addition of thrombin. In some experiments, HUVEC, C3H/10T1/2 or C3H/10T1/2-fLuc cells, that harbor a heat-activated and rapamycin-dependent gene switch for regulating the expression of firefly luciferase transgene expression, were incorporated to the sol phase of the hydrogel...
August 8, 2018: Acta Biomaterialia
U Altanerova, J Jakubechova, K Benejova, P Priscakova, M Pesta, P Pitule, O Topolcan, J Kausitz, M Zduriencikova, V Repiska, C Altaner
The natural behavior of mesenchymal stem cells (MSCs) and their exosomes in targeting tumors is a promising approach for curative therapy. Human tumor tropic mesenchymal stem cells (MSCs) isolated from various tissues and MSCs engineered to express the yeast cytosine deaminase::uracil phosphoribosyl transferase suicide fusion gene (yCD::UPRT-MSCs) released exosomes in conditional medium (CM). Exosomes from all tissue specific yCD::UPRT-MSCs contained mRNA of the suicide gene in the exosome's cargo. When the CM was applied to tumor cells, the exosomes were internalized by recipient tumor cells and in the presence of the prodrug 5-fluorocytosine (5-FC) effectively triggered dose-dependent tumor cell death by endocytosed exosomes via an intracellular conversion of the prodrug 5-FC to 5-fluorouracil...
August 11, 2018: International Journal of Cancer. Journal International du Cancer
Amulya K Saxena, Guenther Klimbacher
OBJECTIVE: Esophagus tissue engineering holds promises to overcome the limitations of the presently employed esophageal replacement procedures. This study investigated 5 animal models for esophageal submucosal glands (ESMG) to identify models appropriate for regenerative medicine applications. Furthermore, this study aimed to measure geometric parameters of ESMG that could be utilized for fabrication of ESMG-specific scaffolds for esophagus tissue engineering applications. METHODS: Ovine, avian, bovine, murine, and porcine esophagus were investigated using Hematoxylin-Eosin (HE), Periodic Acid Schiff (PAS), and Alcian Blue (AB), with AB applied in 3 pH levels (0...
August 10, 2018: Esophagus: Official Journal of the Japan Esophageal Society
Nelli K Bodiford, Steven J P McInnes, Nicolas H Voelcker, Jeffery L Coffer
This work focuses on an evaluation of novel composites of porous silicon (pSi) with the biocompatible polymer ε-polycaprolactone (PCL) for drug delivery and tissue engineering applications. The degradation behavior of the composites in terms of their morphology along with the effect of pSi on polymer degradation was monitored. PSi particles loaded with the drug camptothecin (CPT) were physically embedded into PCL films formed from electrospun PCL fiber sheets. PSi/PCL composites revealed a release profile of CPT (monitored via fluorescence spectroscopy) in accordance with the Higuchi release model, with significantly lower burst release percentage compared to pSi microparticles alone...
August 10, 2018: Biomedical Microdevices
Gudepalya Renukaiah Rudramurthy, Mallappa Kumara Swamy
Nanotechnology advancements have led to the development of its allied fields, such as nanoparticle synthesis and their applications in the field of biomedicine. Nanotechnology driven innovations have given a hope to the patients as well as physicians in solving the complex medical problems. Nanoparticles with a size ranging from 0.2 to 100 nm are associated with an increased surface to volume ratio. Moreover, the physico-chemical and biological properties of nanoparticles can be modified depending on the applications...
August 10, 2018: Journal of Biological Inorganic Chemistry: JBIC
Jun Ishihara, Ako Ishihara, Lambert Potin, Peyman Hosseinchi, Kazuto Fukunaga, Martina Damo, Thomas F Gajewski, Melody A Swartz, Jeffrey A Hubbell
CD40 is an immune co-stimulatory receptor expressed by antigen-presenting cells. Agonistic anti-CD40 antibodies have demonstrated considerable anti-tumor effects yet can also elicit serious treatment-related adverse events, such as liver toxicity, including in man. We engineered a variant that binds extracellular matrix through a super-affinity peptide derived from placenta growth factor-2 (PlGF-2123-144) to enhance anti-CD40's effects when administered locally. Peri-tumoral injection of PlGF-2123-144-anti-CD40 antibody showed prolonged tissue retention at the injection site and substantially decreased systemic exposure, resulting in decreased liver toxicity...
August 10, 2018: Molecular Cancer Therapeutics
Xinxin Zhang, Guanqun Zhan, Ming Jin, Hui Zhang, Jun Dang, Yan Zhang, Zengjun Guo, Yoichiro Ito
BACKGROUND: Radix Gentianae Macrophyllae, commonly known as Qinjiao (in Chinese), is dried roots of medicinal plants that belong to Gentianaceae family and Gentiana genus. It has medically been used for the treatment of various diseases such as arthritis, stroke, facial paralysis, and scapulohumeral periarthritis in China since ancient times. PURPOSE: The aim of this paper is to provide a comprehensive and constructive overview of Qinjiao mainly containing Gentiana macrophylla Pall...
July 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Alaa Aldaadaa, Mustafa Al Qaysi, George Georgiou, Rachel Ma Leeson, Jonathan C Knowles
Phosphate glass is continuing to gain more attention as potential bone substitutes. The ternary (P2 O5 -CaO-Na2 O) is investigated in terms of both physical properties and biocompatibility by doping different percentages of SiO2 and TiO2 . Two groups were prepared; the first has different percentages of TiO2 and SiO2 , whereas the second group compositions have 5 mol% TiO2 and 5 mol% SiO2 being added to compensate the network-forming oxide P2 O5 and the network-modifying oxide CaO. Density, mass loss, pH, DTA, XRD, and cation release experiments were performed to study the physicochemical properties of the compositions, while MG63 and hMS cells were used within in vitro cell culture to study their biocompatibility...
August 2018: Journal of Biomaterials Applications
S S Patil, K C Nune, Rdk Misra
A covalently cross-linked injectable hybrid hydrogel, namely, alginate/poly(amidoamine) (PAMAM), with the objective of cell delivery was innovatively designed and synthesized using tetra-amino-functional PAMAM dendrimer as the cross-linker. With the increase in percentage of PAMAM cross-linker, the pore size and swelling ratio of hydrogels were in the range of 57 ± 18 μm to 88 ± 25 μm and 110 ± 16 to 157 ± 20, respectively. The study of attachment and proliferation of MC3T3-E1 pre-osteoblasts using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay through indirect and direct contact methods indicated a continuous increase in metabolically active live cells with time, implying non-cytotoxicity of the synthesized hydrogel...
August 2018: Journal of Biomaterials Applications
Ranjith Kumar Kankala, Feng-Jun Lu, Chen-Guang Liu, Shan-Shan Zhang, Ai-Zheng Chen, Shi-Bin Wang
In recent times, cartilage defects have been the most common athletic injuries, often leading to dreadful consequences such as osteoarthritis, pain, joint deformities, and other symptoms. It is also evident that damage to articular cartilage is often difficult to recover or self-heal because of poor vascular, nervous, and lymphatic supplies. Moreover, cartilage cells have poor regeneration ability and high maturity. Inspired by these facts and the rapid advances in the field of tissue engineering (TE), we fabricated highly porous three-dimensional (3D) scaffold architectures based on cell-responsive polymeric inks, i...
August 9, 2018: Materials
Riccardo Ferracini, Isabel Martínez Herreros, Antonio Russo, Tommaso Casalini, Filippo Rossi, Giuseppe Perale
Although bone has a high potential to regenerate itself after damage and injury, the efficacious repair of large bone defects resulting from resection, trauma or non-union fractures still requires the implantation of bone grafts. Materials science, in conjunction with biotechnology, can satisfy these needs by developing artificial bones, synthetic substitutes and organ implants. In particular, recent advances in materials science have provided several innovations, underlying the increasing importance of biomaterials in this field...
August 8, 2018: Pharmaceutics
E Marzec, K Pietrucha
Fish collagen (Col) was cross-linked using two methods: dehydrothermal treatment (DHT) and 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) in the presence of N-hydroxy-succinimide (NHS). For the samples marked Col, Col-DHT and Col-EDC/NHS, dielectric properties were measured in the frequency range from 100 Hz to 100 kHz and temperatures from 25 to 145 °C. In the full temperature range, the average values of relative permittivity and dielectric losses for Col samples are lower than those recorded for Col-DHT and Col-EDC/NHS samples...
July 31, 2018: Colloids and Surfaces. B, Biointerfaces
Yong-Chao Jiang, Xiao-Feng Wang, Yi-Yang Xu, Yu-Hui Qiao, Xin Guo, Dong-Fang Wang, Qian Li, Lih-Sheng Turng
During the regeneration of tissues and organs, growth factors (GFs) play a vital role by affecting cell behavior. However, because of the low half-life time and quick degradation of GFs, their stimulations on cells are relatively short and discontinuous. In this study, a releasing scaffold platform, consisting of polycaprolactone (PCL) nanofibers and vascular endothelial growth factor (VEGF)-encapsulated gelatin particles, was developed to extend the influence of GFs on mesenchymal stem cells (MSCs) and endothelial cells (ECs)...
August 10, 2018: Biomacromolecules
Farzad Yousefi, Shital Kandel, Nancy Pleshko
Methacrylated hyaluronic acid (MeHA) has been used extensively in tissue engineering and drug delivery applications. The degree of methacrylation (DM) of HA impacts hydrogel crosslinking, which is of pivotal importance for cell interactions. The methacrylation reaction occurs over several hours, and DM is currently assessed post reaction and after dialysis of the solution, using nuclear magnetic resonance (1 H NMR) data. Thus, there is little control over exact DM in a specific reaction. Here, infrared (IR) spectroscopy in attenuated total reflection (ATR) mode was investigated as an alternate modality for assessment of the DM of HA hydrogels, including during the reaction progression...
August 10, 2018: Applied Spectroscopy
John D Kisiday, Aimee C Colbath, Suwimol Tangtrongsup
Ex vivo induction of chondrogenesis is a promising approach to improve upon the use of bone marrow mesenchymal stem cells (MSCs) for cartilage tissue engineering. This study evaluated the potential to induce chondrogenesis with days of culture in chondrogenic medium for MSCs encapsulated in self-assembling peptide hydrogel. To simulate the transition from preconditioning culture to implantation, MSCs were isolated from self-assembling peptide hydrogel into an individual cell suspension. Commitment to chondrogenesis was evaluated by seeding preconditioned MSCs into agarose and culturing in the absence of the chondrogenic cytokine transforming growth factor beta (TGFβ)...
August 10, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Ramon Rial, J F Armando Soltero, Pedro V Verdes, Zhen Liu, Juan M Ruso
Tissue engineering provides solutions that require medicine to restore damaged tissues or even complete organs. This discipline combines biologically active scaffolds, cells and molecules; being the addition of nanoparticles into the scaffolds one of the techniques that is attracting more interest these days. In this work, Hydroxyapatite nanorods (HA) were added to the network of Gelatin hydrogel (GE), and the particular properties resulting from their interaction were studied. Specifically, viscoelastic properties were characterized as a function of gel and nanoparticle concentration, varying ratios and temperatures...
August 10, 2018: Current Topics in Medicinal Chemistry
Noe Escareno, Antonio Topete, Pablo Taboada, Adrian Daneri-Navarro
The use of colloidal particles as drug delivery carriers holds a great promise in terms of improvement of traditional treatment and diagnosis of human diseases. Nano- and microsized particles of different composition including organic and inorganic materials can be fabricated with a great control over size, shape and surface properties. Nevertheless, only some few formulations have surpassed the benchtop and reached the bedside. The principal obstacle of colloidal drug delivery systems is their poor accumulation in target tissues, organs and cells, mainly by the efficient sequestration and elimination by the mononuclear phagocytic system...
August 10, 2018: Current Topics in Medicinal Chemistry
T G Sahana, P D Rekha
Wound is a growing healthcare challenge affecting several million worldwide. Lifestyle disorders such as diabetes increases the risk of wound complications. Effective management of wound is often difficult due to the complexity in the healing process. Addition to the conventional wound care practices, the bioactive polymers are gaining increased importance in wound care. Biopolymers are naturally occurring biomolecules synthesized by microbes, plants and animals with highest degree of biocompatibility. The bioactive properties such as antimicrobial, immune-modulatory, cell proliferative and angiogenic of the polymers create a microenvironment favorable for the healing process...
August 9, 2018: Molecular Biology Reports
Anna Lucia Gallo, Mauro Pollini, Federica Paladini
Chronic wounds and related infections cause physical and psychological distress in patients, increased mortality, disability and high health care costs. The healing process can be delayed by several factors and in particular by the risk of infections, which can be further complicated by the increasing number of antibiotic-resistant microorganisms. New approaches in wounds management have been encouraged, aiming at preventing infections and improving wound healing. In this scenario, silver has emerged as an ideal antimicrobial agent due to its recognized efficacy against bacteria, viruses and fungi...
August 9, 2018: Journal of Materials Science. Materials in Medicine
Houman Savoji, Brent Godau, Mohsen Sheikh Hassani, Mohsen Akbari
Tremendous progress has been made over the past few decades to develop skin substitutes for the management of acute and chronic wounds. With the advent of tissue engineering and the ability to combine advanced manufacturing technologies with biomaterials and cell culture systems, more biomimetic tissue constructs have been emerged. Synthetic and natural biomaterials are the main constituents of these skin-like constructs, which play a significant role in tissue grafting, the body's immune response, and the healing process...
2018: Frontiers in Bioengineering and Biotechnology
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