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https://www.readbyqxmd.com/read/29166648/different-response-to-hypoxia-of-adipose-derived-multipotent-cells-from-obese-subjects-with-and-without-metabolic-syndrome
#1
Wilfredo Oliva-Olivera, Isabel Moreno-Indias, Leticia Coín-Aragüez, Said Lhamyani, Juan Alcaide Torres, Sonia Fernández-Veledo, Joan Vendrell, Antonio Camargo, Rajaa El Bekay, Francisco José Tinahones
BACKGROUND/OBJECTIVES: Multiple studies suggest that hypoxia, together with inflammation, could be one of the phenomena involved in the onset and progression of obesity-related insulin resistance. In addition, dysfunction of adipose tissue in obese subjects with metabolic syndrome is associated with decreased angiogenesis. However, some subjects with a high body mass index do not develop metabolic abnormalities associated with obesity. The aim of the current study was to examine the neovascular properties of visceral adipose tissue-derived multipotent mesenchymal cells subjected to hypoxia (hypox-visASCs) from normal-weight subjects (Nw) and obese patients with metabolic syndrome (MS) and without metabolic syndrome (NonMS)...
2017: PloS One
https://www.readbyqxmd.com/read/29163479/discriminating-between-interstitial-and-circulating-leukocytes-in-tissues-of-the-murine-oral-mucosa-avoiding-nasal-associated-lymphoid-tissue-contamination
#2
Peter D Bittner-Eddy, Lori A Fischer, Andy A Tu, Daniel A Allman, Massimo Costalonga
Periodontitis is a chronic inflammatory response to a microbial biofilm that destroys bone and soft tissues supporting the teeth. Murine models of periodontitis based on Porphyromonas gingivalis (Pg) colonization have shown that extravasation of leukocytes into oral tissue is critical to driving alveolar bone destruction. Identifying interstitial leukocytes is key to understanding the immunopathogenesis of periodontitis. Here, we describe a robust flow cytometry assay based on intravenous FITC-conjugated anti-mouse CD45 mAb that distinguishes interstitial leukocytes in the oral mucosa of mice from those circulating within the vasculature or in post-dissection contaminating blood...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29162939/intravesicular-administration-of-sodium-hyaluronate-ameliorates-the-inflammation-and-cell-proliferation-of-cystitis-cystica-et-glandularis-involving-interleukin-6-jak2-stat3-signaling-pathway
#3
Yongliang Ni, Shaohua Zhao, Xiaoxuan Yin, Haixin Wang, Qianqian Guang, Guangxia Hu, Yi Yang, Shoubin Jiao, Benkang Shi
Cystitis cystica et glandularis (CCEG) is a chronic cystitis that causes extreme agony in affected patients. However, there are lack of effective conservative treatments. In this study, it is evident that intravesicular sodium hyaluronate (SH) therapy significantly improved the clinical symptoms of CCEG patients and ameliorated the bladder mucosal inflammation and cell proliferation characteristics of the disease. Immunohistochemical staining showed that the staining intensities of hyaluronidase (HYAL 1/2), CD44, IL-6 and phosphorylated signal transducer and activator of transcription 3 (p-Stat3) in bladder mucosal tissue were significantly increased in CCEG patients compared with control patients and that intravesicular SH treatment suppressed these protein expression...
November 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29162741/nuclear-hyaluronidase-2-drives-alternative-splicing-of-cd44-pre-mrna-to-determine-profibrotic-or-antifibrotic-cell-phenotype
#4
Adam C Midgley, Sebastian Oltean, Vincent Hascall, Emma L Woods, Robert Steadman, Aled O Phillips, Soma Meran
The cell surface protein CD44 is involved in diverse physiological processes, and its aberrant function is linked to various pathologies such as cancer, immune dysregulation, and fibrosis. The diversity of CD44 biological activity is partly conferred by the generation of distinct CD44 isoforms through alternative splicing. We identified an unexpected function for the ubiquitous hyaluronan-degrading enzyme, hyaluronidase 2 (HYAL2), as a regulator of CD44 splicing. Standard CD44 is associated with fibrotic disease, and its production is promoted through serine-arginine-rich (SR) protein-mediated exon exclusion...
November 21, 2017: Science Signaling
https://www.readbyqxmd.com/read/29162703/deformability-of-breast-cancer-cells-in-correlation-with-surface-markers-and-cell-rolling
#5
Amina Mohammadalipour, Monica M Burdick, David F J Tees
Although the cancer stem cell (CSC) hypothesis has been around for many years, the reliability of cell-surface markers to classify CSCs has remained debatable. The finding that cancerous cells are significantly more deformable than healthy ones has provided motivation to consider mechanical properties as a possible biomarker for stemness. In this study, using the micropipette aspiration technique, mechanical properties of multiple breast cancer cell lines were investigated and correlated with breast cancer stem cell (BCSC) marker, CD44(+)/CD24(-)/ALDH1(+) The results indicated that Hs578T and MDA-MB-231 cell lines with CD44(+)/CD24(-)/ALDH1(+) phenotype were significantly more deformable than the MDA-MB-468 cell line, which did not express the BCSC marker...
November 21, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29162693/%C3%AE-np63-mediated-regulation-of-hyaluronic-acid-metabolism-and-signaling-supports-hnscc-tumorigenesis
#6
Mirco Compagnone, Veronica Gatti, Dario Presutti, Giovina Ruberti, Claudia Fierro, Elke Katrin Markert, Karen H Vousden, Huiqing Zhou, Alessandro Mauriello, Lucia Anemone, Lucilla Bongiorno-Borbone, Gerry Melino, Angelo Peschiaroli
Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide, and several molecular pathways that underlie the molecular tumorigenesis of HNSCC have been identified. Among them, amplification or overexpression of ΔNp63 isoforms is observed in the majority of HNSCCs. Here, we unveiled a ΔNp63-dependent transcriptional program able to regulate the metabolism and the signaling of hyaluronic acid (HA), the major component of the extracellular matrix (ECM). We found that ∆Np63 is capable of sustaining the production of HA levels in cell culture and in vivo by regulating the expression of the HA synthase HAS3 and two hyaluronidase genes, HYAL-1 and HYAL-3...
November 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29162051/in-vitro-characterization-of-cd133-lo-cancer-stem-cells-in-retinoblastoma-y79-cell-line
#7
Rohini M Nair, Murali Ms Balla, Imran Khan, Ravi Kiran Reddy Kalathur, Paturu Kondaiah, Geeta K Vemuganti
BACKGROUND: Retinoblastoma (Rb), the most common childhood intraocular malignant tumor, is reported to have cancer stem cells (CSCs) similar to other tumors. Our previous investigation in primary tumors identified the small sized cells with low CD133 (Prominin-1) and high CD44 (Hyaluronic acid receptor) expression to be putative Rb CSCs using flow cytometry (FSC(lo)/SSC(lo)/CD133(lo)/CD44(hi)). With this preliminary data, we have now utilized a comprehensive approach of in vitro characterization of Y79 Rb cell line following CSC enrichment using CD133 surface marker and subsequent validation to confirm the functional properties of CSCs...
November 21, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29159714/regulatory-effects-of-dexamethasone-on-nk-and-t-cell-immunity
#8
Liying Chen, Mikael Jondal, Konstantin Yakimchuk
Glucocorticoids (GCs) act via the intracellular glucocorticoid receptor (GR), which can regulate the expression of target genes. With regard to the immune system, GCs may affect both innate and adaptive immunity. Our study analyzed the immunoregulatory effects of dexamethasone (Dex) treatment on splenic T, Treg, NK and NKT cells by treating C57Bl6 mice with various doses of Dex. We observed that treatment with Dex decreased the number of NK cells in the spleen and suppressed their activity. In particular, the expression of both Ly49G and NKG2D receptors was decreased by Dex...
November 20, 2017: Inflammopharmacology
https://www.readbyqxmd.com/read/29158786/fh535-inhibits-proliferation-and-motility-of-colon-cancer-cells-by-targeting-wnt-%C3%AE-catenin-signaling-pathway
#9
Yanyan Chen, Xianping Rao, Kangmao Huang, Xiaoxia Jiang, Haohao Wang, Lisong Teng
Aberrant Wnt/β-catenin pathway activation is frequently observed in human colorectal cancer (CRC) and has become a promising target for CRC treatment. Our study aimed to evaluate the effect of FH535, a small molecule inhibitor of Wnt/β-catenin pathway, on two colon cancer cell lines, HT29 and SW480. We found FH535 significantly inhibited colon cancer cell proliferation in vitro and induced cell cycle arrest. Moreover, FH535 inhibited colon cancer xenograft growth in vivo. Wound-healing assay and Transwell assay revealed that FH535 notably suppressed migration and invasion of SW480 cells...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29156833/targeting-aggressive-prostate-cancer-associated-cd44v6-using-phage-display-selected-peptides
#10
Ying Peng, Austin R Prater, Susan L Deutscher
There is a crucial need to identify new biomarkers associated with aggressive prostate cancer (PCa) including those associated with cancer stem cells (CSCs). CD44v6, generated by alternative splicing of CD44, has been proposed as a CSC biomarker due to its correlation with aggressive PCa disease. We hypothesized that phage display selected peptides that target CD44v6 may serve as theranostic agents for aggressive PCa. Here, a 15 amino acid peptide ("PFT") was identified by affinity selection against a peptide derived from the v6 region of CD44v6...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156825/targeting-p-selectin-blocks-neuroblastoma-growth
#11
Riitta Nolo, Shelley Herbrich, Arvind Rao, Patrick Zweidler-McKay, Sankaranarayanan Kannan, Vidya Gopalakrishnan
Selectins and their ligands have been implicated in tumor growth and progression in carcinomas, but their role in neuroblastoma has not been systematically examined. In the current study we evaluated L-, P- and E-selectin binding to neuroblastoma cells and the expression of some of their known ligands, namely CD44, CD24 and P-selectin glycoprotein ligand-1 (PSGL-1). Genetic loss of PSGL-1 or CD24 and pharmacological inhibition of P-selectin reduced P-selectin binding to neuroblastoma cells in vitro. Targeting P-selectin using specific antibodies promoted a significant reduction in the growth of neuroblastoma tumors in vivo...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156702/post-surgery-fluids-promote-transition-of-cancer-stem-cell-to-endothelial-and-akt-mtor-activity-contributing-to-relapse-of-giant-cell-tumors-of-bone
#12
Flavio Fazioli, Gianluca Colella, Roberta Miceli, Mariano Giuseppe Di Salvatore, Michele Gallo, Serena Boccella, Annarosaria De Chiara, Carlo Ruosi, Filomena de Nigris
Giant cell tumors of bone (GCTB) are rare sarcomas with a high rate of unpredictable local relapse. Studies suggest that surgical methods affect recurrence, supporting the idea that local disease develops from re-growth of residual cancer cells. To identify early prognostic markers of individual risk of recurrence, we evaluated the effect of post-surgery fluids from a cohort of GCTB patients on growth of primary and established sarcoma cell lines, and mice xenograph. Post-surgery fluids increased cell growth and enhanced expression of CD44(++), the principal receptor for the extracellular matrix component hyaluronan and the mesenchymal stem marker CD117(+)...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156374/genetic-profiling-and-surface-proteome-analysis-of-human-atrial-stromal-cells-and-rat-ventricular-epicardium-derived-cells-reveals-novel-insights-into-their-cardiogenic-potential
#13
Sebastian Temme, Daniela Friebe, Timo Schmidt, Gereon Poschmann, Julia Hesse, Bodo Steckel, Kai Stühler, Meik Kunz, Thomas Dandekar, Zhaoping Ding, Payam Akhyari, Artur Lichtenberg, Jürgen Schrader
Epicardium-derived cells (EPDC) and atrial stromal cells (ASC) display cardio-regenerative potential, but the molecular details are still unexplored. Signals which induce activation, migration and differentiation of these cells are largely unknown. Here we have isolated rat ventricular EPDC and rat/human ASC and performed genetic and proteomic profiling. EPDC and ASC expressed epicardial/mesenchymal markers (WT-1, Tbx18, CD73, CD90, CD44, CD105), cardiac markers (Gata4, Tbx5, troponin T) and also contained phosphocreatine...
November 7, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29152317/bone-marrow-derived-multipotent-mesenchymal-stromal-cells-from-horses-after-euthanasia
#14
Carmen Schröck, Carina Eydt, Florian Geburek, Lena Kaiser, Felicitas Päbst, Janina Burk, Christiane Pfarrer, Carsten Staszyk
Allogeneic equine multipotent mesenchymal stromal cells (eMSCs) have been proposed for use in regenerative therapies in veterinary medicine. A source of allogeneic eMSCs might be the bone marrow from euthanized horses. The purpose of this study was to compare in vitro characteristics of equine bone marrow derived eMSC (eBM-MSCs) from euthanized horses (eut-MSCs) and from narcotized horses (nar-MSCs). Eut-MSCs and nar-MSCs showed typical eMSC marker profiles (positive: CD44, CD90; negative: CD11a/CD18 and MHCII) and possessed tri-lineage differentiation characteristics...
November 2017: Veterinary Medicine and Science
https://www.readbyqxmd.com/read/29150704/evaluation-of-immune-mediated-idiosyncratic-drug-toxicity-using-chimeric-hla-transgenic-mice
#15
Takeshi Susukida, Shigeki Aoki, Kotaro Kogo, Sota Fujimori, Binbin Song, Cong Liu, Shuichi Sekine, Kousei Ito
Immune-mediated idiosyncratic drug toxicity (IDT) is a rare adverse drug reaction, potentially resulting in death. Although genome-wide association studies suggest that the occurrence of immune-mediated IDT is strongly associated with specific human leukocyte antigen (HLA) allotypes, these associations have not yet been prospectively demonstrated. In this study, we focused on HLA-B*57:01 and abacavir (ABC)-induced immune-mediated IDT, and constructed transgenic mice carrying chimeric HLA-B*57:01 (B*57:01-Tg) to determine if this in vivo model may be useful for evaluating immune-mediated IDT...
November 17, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/29148014/unfavorable-clinical-implications-of-peripheral-blood-cd44-and-cd54-lymphocytes-in-patients-with-lung-cancer-undergoing-chemotherapy
#16
Zhuanbo Luo, Yun Wang, Yanru Lou, Chao Cao, Richard Hubbard, Ning Xu, Xiaoping Huang
BACKGROUND: There is an unmet need for identification of additional prognostic markers for lung cancer. The aim of this study was to identify novel clinical and immunological predictors of prognosis in lung cancer patients. METHODS: Lymphocyte subsets CD3+, CD4+, CD8+, CD4+/8+, CD25+, CD69+, CD44+ and CD54+ were quantified in peripheral blood using flow cytometry, for 203 newly diagnosed lung cancer patients and 120 healthy controls. RESULTS: The levels of CD3+, CD4+, CD8+, CD4+/CD8+ and CD69+ lymphocytes were significantly lower in patients with lung cancer compared with the healthy control group, while CD54+ and CD44+ lymphocytes were significantly higher...
November 15, 2017: International Journal of Biological Markers
https://www.readbyqxmd.com/read/29146399/label-free-discrimination-analysis-of-de-differentiated-vascular-smooth-muscle-cells-mesenchymal-stem-cells-and-their-vascular-and-osteogenic-progeny-using-vibrational-spectroscopy
#17
Claire Molony, Jennifer McIntyre, Adrian Maguire, Roya Hakimjavadi, Denise Burtenshaw, Gillian Casey, Mariana Di Luca, Bryan Hennelly, Hugh J Byrne, Paul A Cahill
The accumulation of vascular smooth muscle (SMC)-like cells and stem cell-derived myogenic and osteogenic progeny contributes significantly to arteriosclerotic disease. This study established whether label-free vibrational spectroscopy can discriminate de-differentiated 'synthetic' SMCs from undifferentiated stem cells and their myogenic and osteogenic progeny in vitro, compared with conventional immunocytochemical and genetic analyses. TGF-β1- and Jagged1-induced myogenic differentiation of CD44(+)mesenchymal stem cells was confirmed in vitro by immunocytochemical analysis of specific SMC differentiation marker expression (α-actin, calponin and myosin heavy chain 11), an epigenetic histone mark (H3K4me2) at the myosin heavy chain 11 locus, promoter transactivation and mRNA transcript levels...
November 13, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29145336/expression-of-hormone-receptors-in-low-grade-adenosquamous-carcinoma-of-the-breast-a-case-report
#18
Hai-Bo Wu, An-Li Zhang, Wei Wang, Heng Li
RATIONALE: Low-grade adenosquamous carcinoma (LGASC) is a rare subtype of metaplastic breast carcinoma which is generally recognized as a characteristic subgroup of triple-negative breast cancers previously. However, in this study, we reported for the first time a case of LGASC with hormone receptors expression. PATIENT CONCERNS: Pathological analysis of breast tumor specimen obtained by a 42-year-old female patient was performed. Morphologically, it composed of glandular structures with scattered squamous differentiation accompanied by haphazard arrangement of spindle cell stroma...
November 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29144562/mir-5100-increases-the-cisplatin-resistance-of-the-lung-cancer-stem-cells-by-inhibiting-the-rab6
#19
Lawei Yang, Ziying Lin, Yahong Wang, Shenglan Gao, Qinglan Li, Chunyan Li, Wenya Xu, Jie Chen, Tie Liu, Zeqing Song, Gang Liu
Cisplatin-based chemotherapy is the most commonly used treatment regimen for lung cancer. Cancer stem cells (CSCs) are postulated to be important promoters of drug resistance. We previously found that miR-5100 is overexpressed in lung cancer, but it is unknown whether and how miR-5100 regulates cisplatin resistance. Here, we demonstrated that miR-5100 was significantly up-regulated in CD44 + CD133+ lung cancer stem cells (LCSCs) compared with non-CSCs. Additionally, over-expression of miR-5100 increased CSC properties, cell growth and tumor sphere formation in lung cancer cell line A549 or H1299, and that miR-5100 inhibitor significantly increased sensitivity of LCSCs to cisplatin in vitro...
November 16, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29143228/effect-of-exosome-biomarkers-for-diagnosis-and-prognosis-of-breast-cancer-patients
#20
REVIEW
M Wang, S Ji, G Shao, J Zhang, K Zhao, Z Wang, A Wu
PURPOSE: Exosomes are gradually detected as an indicator for diagnosis and prognosis of breast cancer in clinic and a systematic review was conducted. METHODS: A search for clinical studies published before July 1, 2017 was performed. Methods of exosome purification and identification from all studies were extracted. For diagnosis evaluation, the comparison of exosome biomarkers expression between breast cancer patients and healthy women was obtained; for prognosis prediction, the correlation between exosome biomarkers expression and chemotherapy resistance, overall survival (OS), disease-free survival (DFS), recurrence and metastasis of breast cancer was also extracted...
November 15, 2017: Clinical & Translational Oncology
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