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NFAT5 or TonEBP or OREBP

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https://www.readbyqxmd.com/read/30090015/osmotic-and-hypoxic-induction-of-the-complement-factor-c9-in-cultured-human-retinal-pigment-epithelial-cells-regulation-of-vegf-and-nlrp3-expression
#1
Margrit Hollborn, Charlotte Ackmann, Heidrun Kuhrt, Fabian Doktor, Leon Kohen, Peter Wiedemann, Andreas Bringmann
Purpose: Variants of complement factor genes, hypoxia and oxidative stress of the outer retina, and systemic hypertension affect the risk of age-related macular degeneration. Hypertension often results from the high intake of dietary salt that increases extracellular osmolarity. We determined the effects of extracellular hyperosmolarity, hypoxia, and oxidative stress on the expression of complement genes in cultured (dedifferentiated) human RPE cells and investigated the effects of C9 siRNA and C9 protein on RPE cells...
2018: Molecular Vision
https://www.readbyqxmd.com/read/30009821/altered-expression-of-aquaporin-2-in-one-kidney-one-clip-hypertension
#2
Maria Florencia Albertoni Borghese, Sandra Hope, Maria Del Carmen Ortiz, Magalí Barchuk, Camila Kessler, Carlos Davio, Marcelo Vatta, Mónica Majowicz
AIMS: The aim of the present study was to evaluate the regulation of Aquaporin-2 (AQP2) water channel in the kidney of one-kidney, one-clip rats (Goldblatt-1 model). In addition, some mechanisms that underlie the role of AQP2 in the Goldblatt-1 model were evaluated. MAIN METHODS: Sprague-Dawley rats were divided in three groups: control two-kidney, no clip (C, 2 K-NC); nephrectomized one-kidney, no clip (N, 1 K-NC) and Goldblatt one-kidney, one-clip (G, 1 K-1C)...
July 13, 2018: Life Sciences
https://www.readbyqxmd.com/read/29888131/pro-inflammatory-age-rage-signaling-is-activated-during-arousal-from-hibernation-in-ground-squirrel-adipose
#3
Samantha M Logan, Kenneth B Storey
Background: Inflammation is generally suppressed during hibernation, but select tissues (e.g. lung) have been shown to activate both antioxidant and pro-inflammatory pathways, particularly during arousal from torpor when breathing rates increase and oxidative metabolism fueling the rewarming process produces more reactive oxygen species. Brown and white adipose tissues are now understood to be major hubs for the regulation of immune and inflammatory responses, yet how these potentially damaging processes are regulated by fat tissues during hibernation has hardly been studied...
2018: PeerJ
https://www.readbyqxmd.com/read/29847776/suppression-of-pax2-attenuates-allodynia-and-hyperalgesia-through-et-1-etar-nfat5-signaling-in-a-rat-model-of-neuropathic-pain
#4
Lydia Wai Tai, Zhiqiang Pan, Liting Sun, Haobo Li, Pan Gu, Stanley Sau Ching Wong, Sookja K Chung, Chi Wai Cheung
Endothelin-1 (ET-1) and its receptors (ETAR/ETBR) emerge to be a key signaling axis in neuropathic pain processing and are recognized as new therapeutic targets. Yet, little is known on the functional regulation of ET-1 axis during neuropathic pain. Bioinformatics analysis indicated that paired box gene 2 (Pax2) or nuclear factor of activated T-cells 5 (NFAT5), two transcription factors involved in the modulation of neurotransmission, may regulate ET-1. Therefore, we hypothesized that ET-1 axis may be regulated by Pax2 or NFAT5 in the development of neuropathic pain...
August 1, 2018: Neuroscience
https://www.readbyqxmd.com/read/29794013/transcription-factor-nfat5-promotes-migration-and-invasion-of-rheumatoid-synoviocytes-via-coagulation-factor-iii-and-ccl2
#5
Saseong Lee, Jin-Sun Kong, Sungyong You, H Moo Kwon, Seung-Ah Yoo, Chul-Soo Cho, Wan-Uk Kim
Fibroblast-like synoviocytes (FLSs) play a key role in the progression of rheumatoid arthritis (RA) as a primary component of invasive hypertrophied pannus. FLSs of RA patients (RA-FLSs) exhibit cancer-like features, including promigratory and proinvasive activities that largely contribute to joint cartilage and bone destruction. In this study, we hypothesized that the NF of activated T cell 5 (NFAT5), a transcription factor involving tumor invasiveness, would control the migration and invasion of RA-FLSs. Analyses of transcriptomes demonstrated the significant involvement of NFAT5 in locomotion of RA-FLSs and that tissue factor (TF; also known as coagulation factor III) and CCL2 were the major downstream target genes of NFAT5 involving FLS migration and invasion...
July 15, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29739889/farnesoid-x-receptor-is-essential-for-the-survival-of-renal-medullary-collecting-duct-cells-under-hypertonic-stress
#6
Sujuan Xu, Shizheng Huang, Zhilin Luan, Tingyue Chen, Yuanyi Wei, Miaomiao Xing, Yaqing Li, Chunxiu Du, Bing Wang, Feng Zheng, Nanping Wang, Youfei Guan, Jan-Åke Gustafsson, Xiaoyan Zhang
Hypertonicity in renal medulla is critical for the kidney to produce concentrated urine. Renal medullary cells have to survive high medullary osmolarity during antidiuresis. Previous study reported that farnesoid X receptor (FXR), a nuclear receptor transcription factor activated by endogenous bile acids, increases urine concentrating ability by up-regulating aquaporin 2 expression in medullary collecting duct cells (MCDs). However, whether FXR is also involved in the maintenance of cell survival of MCDs under dehydration condition and hypertonic stress remains largely unknown...
May 22, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29700115/cox-2-expression-mediated-by-calcium-tonebp-signaling-axis-under-hyperosmotic-conditions-serves-osmoprotective-function-in-nucleus-pulposus-cells
#7
Hyowon Choi, Weera Chaiyamongkol, Alexandra C Doolittle, Zariel I Johnson, Shilpa S Gogate, Zachary R Schoepflin, Irving M Shapiro, Makarand V Risbud
The nucleus pulposus (NP) of intervertebral discs experiences dynamic changes in tissue osmolarity because of diurnal loading of the spine. TonEBP/NFAT5 is a transcription factor that is critical in osmoregulation as well as survival of NP cells in the hyperosmotic milieu. The goal of this study was to investigate whether cyclooxygenase-2 (COX-2) expression is osmoresponsive and dependent on TonEBP, and whether it serves an osmoprotective role. NP cells up-regulated COX-2 expression in hyperosmotic media. The induction of COX-2 depended on elevation of intracellular calcium levels and p38 MAPK pathway, but independent of calcineurin signaling as well as MEK/ERK and JNK pathways...
June 8, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29661287/prognostic-significance-of-nuclear-factor-of-activated-t-cells-5-expression-in-non-small-cell-lung-cancer-patients-who-underwent-surgical-resection
#8
Hyun Jin Cho, Hwan-Jung Yun, Hee Chul Yang, Soo Jin Kim, Shin Kwang Kang, Chengri Che, Sang Do Lee, Min-Woong Kang
BACKGROUND: Nuclear factor of activated T-cells 5 (NFAT5) is known to be correlated with migration or invasion of tumor cells based on previous in vitro studies. The aim of this study was to analyze the relationship between NFAT5 expression and clinical prognosis in non-small cell lung cancer (NSCLC) patients who underwent surgical resection. MATERIALS AND METHODS: A total of 92 NSCLC patients who underwent surgical resection were enrolled. The tissue microarray core was obtained from surgically resected tumor specimens...
June 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29546053/-positive-regulation-of-rna-metabolic-process-ontology-group-highly-regulated-in-porcine-oocytes-matured-in-vitro-a-microarray-approach
#9
Piotr Celichowski, Mariusz J Nawrocki, Marta Dyszkiewicz-Konwińska, Maurycy Jankowski, Joanna Budna, Artur Bryja, Wiesława Kranc, Sylwia Borys, Sandra Knap, Sylwia Ciesiółka, Michal Jeseta, Karolina Piasecka-Stryczyńska, Ronza Khozmi, Dorota Bukowska, Paweł Antosik, Klaus P Brüssow, Małgorzata Bruska, Michał Nowicki, Maciej Zabel, Bartosz Kempisty
The cumulus-oocyte complexes (COCs) growth and development during folliculogenesis and oogenesis are accompanied by changes involving synthesis and accumulation of large amount of RNA and proteins. In this study, the transcriptomic profile of genes involved in "oocytes RNA synthesis" in relation to in vitro maturation in pigs was investigated for the first time. The RNA was isolated from oocytes before and after in vitro maturation (IVM). Interactions between differentially expressed genes/proteins belonging to "positive regulation of RNA metabolic process" ontology group were investigated by STRING10 software...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29515464/localization-and-expression-of-nuclear-factor-of-activated-t-cells-5-in-myoblasts-exposed-to-pro-inflammatory-cytokines-or-hyperosmolar-stress-and-in-biopsies-from-myositis-patients
#10
Sandrine Herbelet, Elly De Vlieghere, Amanda Gonçalves, Boel De Paepe, Karsten Schmidt, Eline Nys, Laurens Weynants, Joachim Weis, Gert Van Peer, Jo Vandesompele, Jens Schmidt, Olivier De Wever, Jan L De Bleecker
Aims: Regeneration in skeletal muscle relies on regulated myoblast migration and differentiation, in which the transcription factor nuclear factor of activated T-cells 5 (NFAT5) participates. Impaired muscle regeneration and chronic inflammation are prevalent in myositis. Little is known about the impact of inflammation on NFAT5 localization and expression in this group of diseases. The goal of this study was to investigate NFAT5 physiology in unaffected myoblasts exposed to cytokine or hyperosmolar stress and in myositis...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29448950/identification-and-characterization-of-microrna-in-the-lung-tissue-of-pigs-with-different-susceptibilities-to-pcv2-infection
#11
Ping Zhang, Liyuan Wang, Yanping Li, Ping Jiang, Yanchao Wang, Pengfei Wang, Li Kang, Yuding Wang, Yi Sun, Yunliang Jiang
Porcine circovirus type 2 (PCV2) is the primary cause of post-weaning multisystemic wasting syndrome (PMWS) and other PCV-associated diseases. According to our previous RNA-sequencing analysis, the differences in the susceptibility to PCV2 infection depended on the genetic differences between the Laiwu (LW) and Yorkshire × Landrace crossbred (YL) pigs, but the cellular microRNA (miRNA) that are differentially expressed between the LW and YL pigs before and after PCV2 infection remain to be determined. In this study, high-throughput sequencing was performed to determine the abundance and differential expression of miRNA in lung tissues from PCV2-infected and PCV2-uninfected LW and YL pigs...
February 15, 2018: Veterinary Research
https://www.readbyqxmd.com/read/29420225/tonicity-responsive-enhancer-binding-protein-promotes-hepatocellular-carcinogenesis-recurrence-and-metastasis
#12
Jun Ho Lee, Jae Hee Suh, Soo Youn Choi, Hyun Je Kang, Hwan Hee Lee, Byeong Jin Ye, Gap Ryol Lee, Seok Won Jung, Chang Jae Kim, Whaseon Lee-Kwon, Jiyoung Park, Kyungjae Myung, Neung Hwa Park, Hyug Moo Kwon
OBJECTIVES: Hepatocellular carcinoma (HCC) is a common cancer with high rate of recurrence and mortality. Diverse aetiological agents and wide heterogeneity in individual tumours impede effective and personalised treatment. Tonicity-responsive enhancer-binding protein (TonEBP) is a transcriptional cofactor for the expression of proinflammatory genes. Although inflammation is intimately associated with the pathogenesis of HCC, the role of TonEBP is unknown. We aimed to identify function of TonEBP in HCC...
February 2, 2018: Gut
https://www.readbyqxmd.com/read/29345057/sodium-chloride-inhibits-ifn-%C3%AE-but-not-il-4-production-by-invariant-nkt-cells
#13
Dongjin Jeong, Hye Young Kim, Doo Hyun Chung
Invariant NKT (iNKT) cells are a distinct subset of T cells that exert Janus-like functions in vivo by producing IFN-γ and IL-4. Sodium chloride modulates the functions of various immune cells, including conventional CD4+ T cells and macrophages. However, it is not known whether sodium chloride affects iNKT cell function, so we addressed this issue. Sodium chloride inhibited IFN-γ, but not IL-4, production by iNKT cells upon TCR or TCR-independent (IL-12 and IL-18) stimulation in a dose-dependent manner. Consistently, sodium chloride reduced the expression level of tbx21, but not gata-3, in iNKT cells stimulated with TCR engagement or IL-12 + IL-18...
January 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29331043/role-of-mir-203-in-estrogen-receptor-mediated-signaling-in-the-rat-uterus-and-endometrial-carcinoma
#14
Oliver Zierau, Janina Helle, Sabina Schadyew, Yanni Morgenroth, Martin Bentler, Alexander Hennig, Sridar Chittur, Martin Tenniswood, Georg Kretzschmar
The role of microRNAs (miRNA) in estrogen receptor (ER) signaling in the uterus and in endometrial cancer is not well understood. We therefore analyzed miRNA expression in uterine samples from a standard 3-day uterotrophic assay using young female adult rats to identify E2-regulated miRNAs. Microarray analysis identified 47 E2 down-regulated miRNAs including miR-30a, and 25 E2up-regulated miRNAs including miR-672, miR-203, and miR-146b. The strongly E2-upregulated miR-203 was selected for further analysis. miR-203 was deleted in the rat endometrial adenocarcinoma cell line, RUCA-I, using CRISPR/CAS9...
July 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29298866/a-mirna181a-nfat5-axis-links-impaired-t-cell-tolerance-induction-with-autoimmune-type-1-diabetes
#15
Isabelle Serr, Martin G Scherm, Adam M Zahm, Jonathan Schug, Victoria K Flynn, Markus Hippich, Stefanie Kälin, Maike Becker, Peter Achenbach, Alexei Nikolaev, Katharina Gerlach, Nicole Liebsch, Brigitta Loretz, Claus-Michael Lehr, Benedikt Kirchner, Melanie Spornraft, Bettina Haase, James Segars, Christoph Küper, Ralf Palmisano, Ari Waisman, Richard A Willis, Wan-Uk Kim, Benno Weigmann, Klaus H Kaestner, Anette-Gabriele Ziegler, Carolin Daniel
Molecular checkpoints that trigger the onset of islet autoimmunity or progression to human type 1 diabetes (T1D) are incompletely understood. Using T cells from children at an early stage of islet autoimmunity without clinical T1D, we find that a microRNA181a (miRNA181a)-mediated increase in signal strength of stimulation and costimulation links nuclear factor of activated T cells 5 (NFAT5) with impaired tolerance induction and autoimmune activation. We show that enhancing miRNA181a activity increases NFAT5 expression while inhibiting FOXP3+ regulatory T cell (Treg ) induction in vitro...
January 3, 2018: Science Translational Medicine
https://www.readbyqxmd.com/read/29220410/cleavage-of-osmosensitive-transcriptional-factor-nfat5-by-coxsackieviral-protease-2a-promotes-viral-replication
#16
Ye Qiu, Xin Ye, Huifang Mary Zhang, Paul Hanson, Guangze Zhao, Lei Tong, Ronald Xie, Decheng Yang
Nuclear factor of activated T cells 5 (NFAT5)/Tonicity enhancer binding protein (TonEBP) is a transcription factor induced by hypertonic stress in the kidney. However, the function of NFAT5 in other organs has rarely been studied, even though it is ubiquitously expressed. Indeed, although NFAT5 was reported to be critical for heart development and function, its role in infectious heart diseases has remained obscure. In this study, we aimed to understand the mechanism by which NFAT5 interferes with infection of Coxsackievirus B3 (CVB3), a major cause of viral myocarditis...
December 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/29196933/osmolyte-type-and-the-osmolarity-level-affect-chondrogenesis-of-mesenchymal-stem-cells
#17
Sorour Ahmadyan, Mahboubeh Kabiri, Hana Hanaee-Ahvaz, Ali Farazmand
The inductive effects of increased osmolarity on chondrogenesis are well approved. However, the effects of the osmolyte agent invoked to induce hyperosmolarity are largely neglected. Herein, we scrutinized how hyperosmotic conditions acquired by addition of different osmolytes would impact chondrogenesis. We briefly assessed whether such conditions would differentially affect hypertrophy and angiogenesis during MSC chondrogenesis. Chondrogenic and hypertrophic marker expression along with VEGF secretion during adipose-derived (AD)-MSC chondrogenesis under three osmolarity levels (350, 450, and 550 mOsm) using three different osmolytes (NaCl, sorbitol, and PEG) were assessed...
June 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29158465/tonicity-responsive-enhancer-binding-protein-mediates-hyperglycemia-induced-inflammation-and-vascular-and-renal-injury
#18
Soo Youn Choi, Sun Woo Lim, Shabnam Salimi, Eun Jin Yoo, Whaseon Lee-Kwon, Hwan Hee Lee, Jun Ho Lee, Braxton D Mitchell, Satoru Sanada, Afshin Parsa, Hyug Moo Kwon
Diabetic nephropathy (DN) has become the single leading cause of ESRD in developed nations. Bearing in mind the paucity of effective treatment for DN and progressive CKD, novel targets for treatment are sorely needed. We previously reported that increased activity of tonicity-responsive enhancer-binding protein (TonEBP) in monocytes was associated with early DN in humans. We now extend these findings by testing the hypotheses that TonEBP in macrophages promotes hyperglycemia-mediated proinflammatory activation and chronic renal inflammation leading to DN and CKD, and TonEBP genetic variability in humans is associated with inflammatory, renal, and vascular function-related phenotypes...
February 2018: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29150563/nfat5-regulated-macrophage-polarization-supports-the-proinflammatory-function-of-macrophages-and-t-lymphocytes
#19
Mónica Tellechea, Maria Buxadé, Sonia Tejedor, Jose Aramburu, Cristina López-Rodríguez
Macrophages are exquisite sensors of tissue homeostasis that can rapidly switch between pro- and anti-inflammatory or regulatory modes to respond to perturbations in their microenvironment. This functional plasticity involves a precise orchestration of gene expression patterns whose transcriptional regulators have not been fully characterized. We had previously identified the transcription factor NFAT5 as an activator of TLR-induced responses, and in this study we explore its contribution to macrophage functions in different polarization settings...
January 1, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29052520/upregulation-of-dars2-by-hbv-promotes-hepatocarcinogenesis-through-the-mir-30e-5p-mapk-nfat5-pathway
#20
COMPARATIVE STUDY
Xian Qin, Changsheng Li, Tao Guo, Jing Chen, Hai-Tao Wang, Yi-Tao Wang, Yu-Sha Xiao, Jun Li, Pengpeng Liu, Zhi-Su Liu, Quan-Yan Liu
BACKGROUND: Infection with the hepatitis B virus (HBV) is closely associated with the development of hepatocellular carcinoma (HCC). The osmoregulatory transcription factor nuclear factor of activated T-cells 5 (NFAT5) has been shown to play an important role in the development of many types of human cancers. The role of NFAT5 in HBV-associated HCC has never previously been investigated. METHODS: We compared expression profiles of NFAT5, DARS2 and miR-30e-5p in HCC samples, adjacent nontumor tissues and different hepatoma cell lines by quantitative real-time polymerase chain reaction and /or Western blot...
October 19, 2017: Journal of Experimental & Clinical Cancer Research: CR
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