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three-dimensional culture

Ning Li, Chengzhi Wang, Shujin Sun, Chen Zhang, Dongyuan Lü, Qin Chen, Mian Long
Endothelial cells (ECs) are mechanosensitive cells undergoing morphological and functional changes in space. Ground-based study has provided a body of evidences about how ECs can respond to the effect of simulated microgravity, however, these results need to be confirmed by spaceflight experiments in real microgravity. In this work, we cultured EA.hy926 ECs on board the SJ-10 Recoverable Scientific Satellite for 3 and 10 days, and analyzed the effects of space microgravity on the ECs. Space microgravity suppressed the glucose metabolism, modulated the expression of cellular adhesive molecules such as ICAM-1, VCAM-1, and CD44, and depressed the pro-angiogenesis and pro-inflammation cytokine secretion...
2018: Frontiers in Physiology
Xuanzhi Wang, Xinda Li, Xingliang Dai, Xinzhi Zhang, Jing Zhang, Tao Xu, Qing Lan
Glioblastoma (GBM), with rich blood vessels and high invasiveness, is the most common malignant primary brain tumor. The current treatment strategies are less effective, resulting in tumor recurrence. Tumor angiogenesis plays an important role in the occurrence, development and metastasis of GBM. Currently, GBM has been treated by inhibiting tumor angiogenesis. In-depth study of tumor angiogenesis is of great significance for the treatment of GBM. Recent studies have shown that glioma stem cells (GSCs) are involved in tumor vascularization by secreting vascular endothelial growth factor (VEGF)...
August 7, 2018: Colloids and Surfaces. B, Biointerfaces
Arianna Magon, Cristina Arrigoni, Tiziana Roveda, Paola Grimoldi, Federica Dellafiore, Marco Moia, Kehinde O Obamiro, Rosario Caruso
INTRODUCTION: Oral Anticoagulation therapy (OAC) is highly effective in the management of thromboembolic disorders. An adequate level of knowledge is important for self-management and optimizing clinical outcomes. The Anticoagulation Knowledge Tool (AKT) was developed to assess OAC knowledge and caters for both patients prescribed direct oral anticoagulants or vitamin K antagonist (VKA). However, evidence regarding its psychometric proprieties, validity and reliability are unavailable in non-English speaking settings...
2018: PloS One
Julia C Young, Michelle R Quayle, Justin W Adams, John F Bertram, Paul G McMenamin
The practical aspect of human developmental biology education is often limited to the observation and use of animal models to illustrate developmental anatomy. This is due in part to the difficulty of accessing human embryonic and fetal specimens, and the sensitivity inherent to presenting these specimens as teaching materials. This report presents a new approach using three-dimensional (3D) printed replicas of actual human materials in practical classes, thus allowing for the inclusion of accurate examples of human developmental anatomy in the educational context...
August 14, 2018: Anatomical Sciences Education
Jinsong Wu, Runyuan Zhao, Jing Lin, Baohua Liu
Integrin contributes to the maintenance of cell adhesion. In turn, cell adhesion triggers certain integrin signaling cascades, and influences cell biological behavior. In the present study, we explored the role and mechanism of integrin β4 in the DNA damage response in colorectal cancer (CRC) using a three‑dimensional (3D) cell culture model. Under 3D culture condition, dispersed CRC cells automatically formed multicellular spheroids, which consisted of layers of cells with cell junctions commonly distributed...
August 6, 2018: Oncology Reports
Lorenz J Maier, Stefan M Kallenberger, Katharina Jechow, Marcel Waschow, Roland Eils, Christian Conrad
Three-dimensional protein localization intricately determines the functional coordination of cellular processes. The complex spatial context of protein landscape has been assessed by multiplexed immunofluorescent staining or mass spectrometry, applied to 2D cell culture with limited physiological relevance or tissue sections. Here, we present 3D SPECS, an automated technology for 3D Spatial characterization of Protein Expression Changes by microscopic Screening. This workflow comprises iterative antibody staining, high-content 3D imaging, and machine learning for detection of mitoses...
August 13, 2018: Molecular Systems Biology
Tatiana Chadid, Andrew Morris, Alexandra Surowiec, Scott Robinson, Maiko Sasaki, Jacques Galipeau, Brian P Pollack, Luke P Brewster
OBJECTIVE: Regenerative medicine seeks to stall or to reverse the pathologic consequences of chronic diseases. Many people with diabetes have peripheral arterial disease (PAD), which increases their already high risk of major amputation. Cellular therapies are a promising regenerative medicine approach to PAD that can be used to focally inject regenerative cells to endangered tissue beds. Mesenchymal stem cells (MSCs) are known to promote tissue regeneration through stromal support and paracrine stimulation of new blood vessels (angiogenesis)...
August 10, 2018: Journal of Vascular Surgery
M Orditura, C M Della Corte, A Diana, V Ciaramella, E Franzese, V Famiglietti, I Panarese, R Franco, A Grimaldi, A Lombardi, M Caraglia, A Santoriello, E Procaccini, E Lieto, E Maiello, F De Vita, F Ciardiello, F Morgillo
Two inhibitors of phosphatidylinositol 3-kinase (PI3K) pathway taselisib, targeting the mutant PI3K-subunit-alpha (PI3KA) and ipatasertib, AKT-inhibitor, are currently under clinical investigation in breast cancer (BC) patients. We have previously demonstrated the anti-tumor efficacy of these anti-PI3K/AKT-inibitors in combination with anti-microtubule drugs in human BC cell lines, through a complete cytoskeleton disorganization. In this work, we generated ex-vivo three-dimensional (3D) cultures from human BC as a model to test drug efficacy and to identify new molecular biomarkers for selection of BC patients suitable for anti-PI3K/AKT-inibitors treatment...
August 4, 2018: Breast: Official Journal of the European Society of Mastology
Afu Fu, Yu Ming Peh, Weida Ngan, Na Wei, Kathy Qian Luo
Treating systemic metastases at the micrometastatic stage is a potential strategy to inhibit cancer metastasis. This study aims to establish an apoptosis sensor-based platform for rapid, effective, and non-invasive identification of drugs that can inhibit the proliferation of micrometastatic cancer cells. We stably transfected the plasmid DNA encoding the fluorescence resonance energy transfer (FRET)-based caspase-3 sensor into highly metastatic melanoma B16F10 cells. The resulting B16F10-C3 cells were applied for screening of anti-proliferative and pro-apoptotic drugs in two-dimensional (2D) monolayer, three-dimensional (3D) spheroids, and zebrafish xenotransplantation tumors...
August 13, 2018: Biotechnology and Bioengineering
Frank Alifui-Segbaya, Jasper Bowman, Alan R White, Sony Varma, Graham J Lieschke, Roy George
The paucity of information on the biological risks of photopolymers in additive manufacturing or three-dimensional printing (3DP) is a major challenge for the uptake of the technology in the construction of medical devices in dentistry. In this paper, the biocompatibility of additively manufactured methacrylates are evaluated using the innovative zebrafish (Danio rerio) embryo model. Data obtained confirmed gradations of toxicity primarily influenced by ethanol treatment, exposure scenarios, and extraction vehicles...
August 9, 2018: Acta Biomaterialia
Yasemin Budama-Kilinc, Rabia Cakir-Koc, Serda Kecel-Gunduz, Tolga Zorlu, Yagmur Kokcu, Bilge Bicak, Zeynep Karavelioglu, Aysen E Ozel
Papain is a protease enzyme with therapeutic properties that are very valuable for medical applications. Poly(ε-caprolactone) (PCL) is an ideal polymeric carrier for controlled drug delivery systems due to its low biodegradability and its high biocompatibility. In this study, the three-dimensional structure and action mechanism of papain were investigated by in vitro and in silico experiments using molecular dynamics (MD) and molecular docking methods to elucidate biological functions. The results showed that the size of papain-loaded PCL nanoparticles (NPs) and the polydispersity index (PDI) of the NPs were 242...
August 10, 2018: Journal of Fluorescence
Xiuhua Xu, J Julie Kim, Yinuo Li, Jia Xie, Changshun Shao, Jian-Jun Wei
Uterine leiomyomas (ULM) grow under high oxidative stress due to a hypoxic microenvironment and defects in redox metabolism. AKT is one major pathway activated by reactive oxygen species (ROS) that maintains ULM growth and survival. We previously reported that AKT inactivated by AKT inhibitors can significantly induce cellular senescence in ULM cells. Since some miRNAs are induced by AKT inhibitors in an ROS-dependent manner, we proposed that these miRNAs may modulate AKT function and cellular senescence in ULM...
August 10, 2018: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
Kotomi Seno, Yasuhisa Munakata, Michiya Sano, Ryouka Kawahara-Miki, Hironori Takahashi, Akihide Ohkuchi, Hisataka Iwata, Takehito Kuwayama, Koumei Shirasuna
BACKGROUND: Three-dimensional (3D) culture changes cell characteristics and function, suggesting that 3D culture provides a more physiologically relevant environment for cells compared with 2D culture. We investigated the differences in cell functions depending on the culture model in human trophoblast cells (Sw.71). METHODS: Sw.71 cells were incubated in 2D monolayers or simple 3D spheroids. After incubation, cells were corrected to assess RNA-seq transcriptome or protein expression, and culture medium were corrected to detect cytokines...
August 8, 2018: International Journal of Molecular Sciences
Christoph Eilenberger, Sebastian Rudi Adam Kratz, Mario Rothbauer, Eva-Kathrin Ehmoser, Peter Ertl, Seta Küpcü
The assessment of drug-dose responses is vital for the prediction of unwanted toxicological effects in modern medicine. Three-dimensional (3D) cell cultures techniques can provide in vivo -like spheroids and microtissues that resemble natural tumor function. However, formation of necrotic core and diffusion limitation of chemical compounds within these models can reduce the reproducibility and precision of standard bioassay protocols used to test two-dimensional (2D) cell cultures. Nonetheless, the accurate prediction of detrimental effects of test compounds based on functional bioassays is essential for the development of new efficient therapeutic strategies...
2018: MethodsX
Florian Janker, Walter Weder, Jae-Hwi Jang, Wolfgang Jungraithmayr
Lung cancer is the leading cause of cancer-related mortality worldwide. Animal models are key in analyzing cancer biology and therapy evaluation. We here compared relevant non-genetic lung cancer models with regard to tumor induction period, incidence, morbidity and mortality rate and the immunological composition of primary tumors and the occurrence of tertiary lymphoid organs (TLO): (I) intraperitoneal Urethane injection (1g/kg), (II) Lewis lung carcinoma (LLC) cell line model (intravenous or subcutaneous), and (III) ex vivo three-dimensional (3D) primary cell culture model established from subcutaneously developed LLC-induced tumors...
July 17, 2018: Oncotarget
Dominique Bremond-Gignac, Henri Copin, Moncef Benkhalifa
Ocular surface diseases with limbal insufficiency represent a therapeutic challenge for restoring vision. This corneal deficiency includes both classical ocular diseases (as chemical burns) and rare ocular diseases (as congenital aniridia and ocular cicatricial pemphigoid). Areas covered: Our understanding of limbal epithelial stem cells (LESCs) has increased the potential for treatment options. Pharmacological treatment strategies (as regenerating agent ophthalmic solutions) and especially surgical treatment strategies are available...
August 9, 2018: Expert Opinion on Biological Therapy
Bethany R Hughes, Marziye Mirbagheri, Stephen D Waldman, Dae Kun Hwang
Cell-cell communication plays a critical role in a myriad of processes, such as homeostasis, angiogenesis, and carcinogenesis, in multi-cellular organisms. Monolayer cell models have notably improved our understanding of cellular interactions. However, the cultured cells on the planar surfaces adopt a two-dimensional morphology, which poorly imitates cellular organization in vivo, providing physiologically-irrelevant cell responses. Non-planar surfaces comprising various patterns have demonstrated great abilities in directing cellular growth and producing different cell morphologies...
August 6, 2018: Acta Biomaterialia
Yun Luo, Shangsi Chen, Yufei Shi, Jun Ma
In this study, strontium substituted hydroxyapatite (Sr-HAP) was synthesized using collagen type I and citrate as bi-templates and the obtained nanoparticles with high similarity to natural bone minerals were made into composite scaffolds with inter-connected porous structure using a three-dimensional (3D) printing technique. A calcium-deficient structure of HAP phase was caused by doping Sr which was verified by FT-IR, XRD, SEM and TEM. The Sr/(Sr+Ca) molar ratio in Sr-HAP nanoparticles was 5.8% estimated by EDX...
August 9, 2018: Biomedical Materials
Chen Wang, Ping Wu, Lei Yao, Jinghua Xue, Liangxiong Xu, Hanxiang Li, Wangqiu Deng, Xiaoyi Wei
Four new peptaibiotics, acremotins A-D (1-4) featuring three α,α-dialkylated amino acid-imino acid motifs and an unreduced C-terminal residue, along with the known peptaibiotic XR586 (5) were isolated from the solid cultures of the soil-derived fungus Acremonium persicinum SC0105. Their primary structures were characterized by detailed analysis of the HRESIMS/MS fragmentation pattern combined with comprehensive interpretation of the 1D and 2D NMR spectroscopic data. The absolute configurations of amino acid residues were determined by the advanced Marfey's method...
August 8, 2018: Journal of Antibiotics
Hiroyuki Imai, Wataru Fujii, Ken Takeshi Kusakabe, Yasuo Kiso, Etsuro Ono, Kiyoshi Kano
Cultured cells are generally observed through the bottom of dishes or flasks using an inverted microscope. Two-dimensional and horizontal observation is insufficient for histological analysis of several cell lines, such as embryonic stem cells or cancer cells, because they form three-dimensional colonies. In the present study, we aimed to establish a more informative method for analysis of such stereoscopic cultured cells. We cultured mouse embryonic stem cells using a temperature-sensitive culture dish, embedded these cells in paraffin, and successfully observed vertical sections of embryonic stem cells...
August 9, 2018: Journal of Veterinary Medical Science
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