Read by QxMD icon Read

three-dimensional culture

Christian Krug, Anita Beer, Bastian Hartmann, Carina Prein, Hauke Clausen-Schaumann, Thomas Holzbach, Attila Aszodi, Riccardo Enzo Giunta, Maximilian Michael Saller, Elias Volkmer
Adipose-derived multipotent stem/progenitor cells (ASPCs) were shown to be ideal candidates for cell-based regenerative therapies. Yet, despite their huge potential, successful clinical applications are still rare. It was suggested that the efficacy of ASPCs at the recipient site depends on the vehicle of cell delivery. In this study, for preparation of a murine critical-size nerve defect model, we assessed the commercially available fibrin gel (ARTISS) as a potential cell carrier. In a thorough in vitro analysis, we investigated cell-fibrin interactions, and analyzed the distribution and the long-term behavior of ASPCs cultivated in fibrin gel under normoxic and hypoxic conditions...
December 4, 2018: Journal of Tissue Engineering and Regenerative Medicine
Naser Mobarra, Masoud Soleimani, Majid Ghayour-Mobarhan, Samaneh Safarpour, Gordon A Ferns, Reza Pakzad, Parvin Pasalar
A suitable alternative strategy for liver transplantation is the use of nanofibrous scaffolds together with stem cells. In this study, a random hybrid of poly-l-lactic acid (PLLA) and poly(ε-caprolactone) (PCL) was used as a three-dimensional (3D) culture for differentiation of hepatocyte-like cells and compared with routine culture (two-dimensional [2D]). The expression of the endodermal marker, forkhead box A2 (FOXA2), was assessed on Day 3 and the hepatic markers; albumin (ALB), α-1 antitrypsin (AAT), and cytokeratin-18 (CK-18) were evaluated on Day 18 using quantitative polymerase chain reaction qPCR...
December 4, 2018: Journal of Cellular Physiology
Norman Truong, Evan Kurt, Sasha Cai Lesher-Perez, Tatiana Segura
Gene delivery using injectable hydrogels can serve as a potential method for regulated tissue regeneration in wound healing. Our microporous annealed particle (MAP) hydrogel has been shown to promote cellular infiltration in both skin and brain wounds, while reducing inflammation. Although the scaffold itself can promote healing, likely other signals will be required to promote healing of hard to treat wounds. Gene delivery is one approach to introduce desired bioactive signals. In this study, we investigated how the properties of MAP hydrogels influence non-viral gene delivery of polymer condensed plasmid to cells seeded within the MAP gel...
December 4, 2018: Bioconjugate Chemistry
Luciana H Osaki, Kevin A Bockerstett, Chun Fung Wong, Eric L Ford, Blair B Madison, Richard J DiPaolo, Jason C Mills
Chronic inflammation of the gastric mucosa, often caused by autoimmune gastritis and/or infection with Helicobacter pylori, can lead to atrophy of acid-secreting parietal cells with metaplasia of remaining cells. The histological pattern marks a critical step in the progression from chronic gastritis to gastric cancer, yet underlying mechanism(s) of inflammation-induced cell death of gastric epithelial cells are poorly understood. We investigated direct effects of a type 1 cytokine associated with autoimmunity and infection, interferon-γ (IFN-γ), on gastric epithelial cells...
December 3, 2018: Journal of Pathology
Tobias Lange, Su Jung Oh-Hohenhorst, Simon A Joosse, Klaus Pantel, Oliver Hahn, Tobias Gosau, Sergey A Dyshlovoy, Jasmin Wellbrock, Susanne Feldhaus, Hanna Maar, Renate Gehrcke, Martina Kluth, Ronald Simon, Thorsten Schlomm, Hartwig Huland, Udo Schumacher
Here we describe the establishment and characterization of an AR+, PSMA+, ERG+, PTEN-/- , CHD1+/- patient-derived xenograft (PDX) model termed 'C5', which has been developed from a 60 years old patient suffering from castration-resistant prostate cancer (CRPC). The patient underwent radical prostatectomy, showed early tumor marker PSA recurrence and, one year after surgery, abiraterone resistance. Subcutaneous C5 tumors can be serially transplanted between mice and grow within ~90 days to 1.5-2 cm³ tumors in SCID Balb/c mice (take rate 100%), NOD-scid IL2Rgnull (NSG) mice (100%) and C57BL/6 pfp-/- /rag2-/- mice (66%)...
December 3, 2018: Scientific Reports
Eirikur Briem, Zuzana Budkova, Anna Karen Sigurdardottir, Bylgja Hilmarsdottir, Jennifer Kricker, Winston Timp, Magnus Karl Magnusson, Gunnhildur Asta Traustadottir, Thorarinn Gudjonsson
MicroRNAs regulate developmental events such as branching morphogenesis, epithelial to mesenchymal transition (EMT) and its reverse process mesenchymal to epithelial transition (MET). In this study, we performed small RNA sequencing of a breast epithelial progenitor cell line (D492), and its mesenchymal derivative (D492M) cultured in three-dimensional microenvironment. Among the most downregulated miRNAs in D492M was miR-203a, a miRNA that plays an important role in epithelial differentiation. Increased expression of miR-203a was seen in D492, concomitant with increased complexity of branching...
November 30, 2018: Mechanisms of Development
Mark R Cronan, Molly A Matty, Allison F Rosenberg, Landry Blanc, Charlie J Pyle, Scott T Espenschied, John F Rawls, Véronique Dartois, David M Tobin
A central and critical structure in tuberculosis, the mycobacterial granuloma consists of highly organized immune cells, including macrophages that drive granuloma formation through a characteristic epithelioid transformation. Difficulties in imaging within intact animals and caveats associated with in vitro assembly models have severely limited the study and experimental manipulation of mature granulomas. Here we describe a new ex vivo culture technique, wherein mature, fully organized zebrafish granulomas are microdissected and maintained in three-dimensional (3D) culture...
December 2018: Nature Methods
Cheng-Che Lee, Yun-Ching Cheng, Chi-Yen Chang, Chi-Min Lin, Jang-Yang Chang
MEC-17, a newly identified alpha-tubulin-N-acetyltransferase 1, serves as the major α-tubulin acetyltransferase to promote α-tubulin acetylation in vitro and in vivo. Alteration of α-tubulin acetylation may be involved in morphology regulation, cell migration, and tumour metastasis. However, MEC-17's role in cell physiology and its effect on epithelial-mesenchymal transition (EMT) and cell polarity remain elusive. In the present study, we characterized the overexpressed or downregulated cell models through gene targeting as MEC-17 gain- or loss-of-function...
November 30, 2018: Scientific Reports
Robert Mathew, Neelima Renjith, P S Mathuranath
Background: Cube copying test is often used as screening test for dementia. However, there is a paucity of an effectively scoring system, and very little is known about how healthy older adults perform on this test. In this study, we present a modified scoring system for the wire-cube copying, evaluate the performance of cognitively unimpaired elderly individuals, and generate norms on community-dwelling older adults. Materials and Methods: The task consisted of copying a three-dimensional printed cube (i...
November 2018: Neurology India
Agnieska Brazovskaja, Barbara Treutlein, J Gray Camp
Three-dimensional (3D) tissues grown in culture from human stem cells offer the incredible opportunity to analyze and manipulate human development, and to generate patient-specific models of disease. Methods to sequence DNA and RNA in single cells are being used to analyze these so-called 'organoid' systems in high-resolution. Single-cell transcriptomics has been used to quantitate the similarity of organoid cells to primary tissue counterparts in the brain, intestine, liver, and kidney, as well as identify cell-specific responses to environmental variables and disease conditions...
November 29, 2018: Current Opinion in Biotechnology
Viktoriya Sokolova, Leonardo Rojas-Sánchez, Nataniel Białas, Nina Schulze, Matthias Epple
The transfer of nucleic acids into living cells, i.e. transfection, is a major technique in current molecular biology and medicine. As nucleic acids alone are not able to penetrate the cell membrane, an efficient carrier is needed. Calcium phosphate nanoparticles can serve as carrier due to their biocompatibility, biodegradability and high affinity to nucleic acids like DNA or RNA. Their application was extended here from two-dimensional (2D) to three-dimensional (3D) cell culture models, including co-cultures...
November 29, 2018: Acta Biomaterialia
Soojung Hahn, Mi Sun Kim, Seon Young Choi, Sukin Jeong, JooHyun Jee, Han Kyung Kim, Sang Yun Jeong, Hyunsoo Shin, Hyoung Sun Kim, Joon Seong Park, Jongman Yoo
An organoid is a complex, multi-cell three-dimensional (3D) structure that contains tissue-specific cells. Epithelial stem cells, which are marked by leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5), have the potential for self-renewal and expansion as organoids. However, in the case of intestinal organoids from Lgr5-EGFP-IRES-CreERT2 transgenic mice, in vitro expansion of the Lgr5 expression is limited in a culture condition supplemented with essential proteins, such as epidermal growth factor (E), noggin (N), and R-spondin 1 (R)...
November 28, 2018: Biochemical and Biophysical Research Communications
Minnie Malik, Joy Britten, Mostafa Borahay, James Segars, William H Catherino
OBJECTIVE: To observe the antifibroid effects of therapeutic concentrations of simvastatin, which interferes with cholesterol biosynthesis, a known precursor of five major classes of steroid hormones, including progesterone and estrogen, which play a major role in the development and growth of uterine leiomyomas. DESIGN: Two-dimensional and three-dimensional cell culture study of immortalized human leiomyoma and patient-matched myometrium cells treated with simvastatin...
December 2018: Fertility and Sterility
Elham Abedin, Roya Lari, Nasser Mahdavi Shahri, Masoud Fereidoni
Extracellular matrix (ECM) controls cellular behaviors such as proliferation, migration, and differentiation. The decellularized matrix of mammalian tissues has been used as a scaffold for the repair and reconstruction of tissue defects. In this study, for the first time demineralized and decellularized human epiphyseal bone matrix was used as a scaffold for a bone generation. Human epiphyseal bone was demineralized by hydrochloric acid and then decellularized by three methods of physical (slow freezing and snap freeze-thaw), enzymatic (trypsin 0...
December 2018: Tissue & Cell
Fiona Louis, Shiro Kitano, João F Mano, Michiya Matsusaki
Although adipose tissue is one of the most abundant tissues of the human body, its reconstruction remains a competitive challenge. The conventional in vitro two or three-dimensional (2D or 3D) models of mature adipocytes unfortunately lead to their quick dedifferentiation after one week, and complete differentiation of adipose derived stem cells (ADSC) usually requires more than one month. In this context, we developed biomimetic 3D adipose tissues with high density collagen by mixing type I collagen microfibers with primary mouse mature adipocytes or human ADSC in transwells...
November 28, 2018: Acta Biomaterialia
Delphine Morales, Florian Lombart, Agathe Truchot, Pauline Maire, Marwa Hussein, Warda Hamitou, Pascale Vigneron, Antoine Galmiche, Catherine Lok, Muriel Vayssade
Melanoma cell sensitivity to targeted therapy molecules is dependent on the tumor microenvironment (cell-cell and cell-extracellular matrix interactions). Three dimensional (3D) in vitro cell culture systems better reflect the native structural architecture of tissues and are attractive to investigate cellular interactions. We have developed and compared several metastatic melanoma models: melanoma cells (SK-MEL-28 and SK-MEL-3, BRAF V600E mutant and SK-MEL-2 BRAF wt) cultured as a monolayer (2D) and co-cultured on 3D dermal equivalents with fibroblasts to better unravel factors modulating cell sensitivity to a BRAF inhibitor (BRAFi, vemurafenib)...
December 1, 2018: Tissue Engineering. Part A
Ali AbuMadighem, Ronnie Solomon, Alina Stepanovsky, Joseph Kapelushnik, QingHua Shi, Eckart Meese, Eitan Lunenfeld, Mahmoud Huleihel
Aggressive chemotherapy may lead to permanent male infertility. Prepubertal males do not generate sperm, but their testes do contain spermatogonial cells (SPGCs) that could be used for fertility preservation. In the present study, we examined the effect of busulfan (BU) on the SPGCs of immature mice, and the possible induction of the survivor SPGCs to develop spermatogenesis in 3D in-vitro culture. Immature mice were injected with BU, and after 0.5⁻12 weeks, their testes were weighed and evaluated histologically compared to the control mice...
November 29, 2018: International Journal of Molecular Sciences
Xi Chen, Wen Jiang, Ayman Ahmed, Clare S Mahon, Markus Müllner, Bocheng Cao, Tian Xia
Three-dimensional (3D) hepatocyte microtissues (MT), also known as spheroids, have proven to be advantageous in providing more accurate information and physiologically relevant and predictive data for liver-related in vivo tests; therefore, spheroids have increasingly been used to study hepatotoxicity, drug delivery to the liver, and tissue engineering. However, variabilities in the generation of 3D MT remain a major challenge. Methods which encapsulate and protect hepatocytes offer a promising pathway in prolonging cell survival, as well as maintaining its pristine cell functions...
November 30, 2018: Chemical Research in Toxicology
Weimin Jiang, Weikang Zhang, Dongdong Li, Ye Song
The two-dimensional (2D) cell culture model is currently used to study cellular processes and drug screening for human diseases. However, the growth of cells is affected by many factors. For conventional 2D cell culture, many of the difficulties are encountered in accurately replicating the cell function in three-dimensional (3D) tissues. Compared with 2D cell culture, much attention is paid to the cell-to-cell and cell-matrix interactions for 3D cell culture systems, which can more closely mimic the growth environment for cultured cells...
November 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Pratima Basak, Sumanta Chatterjee, Vasudeva Bhat, Alice Su, Hyerang Jin, Victoria Lee-Wing, Qian Liu, Pingzhao Hu, Leigh C Murphy, Afshin Raouf
BACKGROUND/AIMS: Blocking estrogen signaling with endocrine therapies (Tamoxifen or Fulverstrant) is an effective treatment for Estrogen Receptor-α positive (ER+) breast cancer tumours. Unfortunately, development of endocrine therapy resistance (ETR) is a frequent event resulting in disease relapse and decreased overall patient survival. The long noncoding RNA, H19, was previously shown to play a significant role in estrogen-induced proliferation of both normal and malignant ER+ breast epithelial cells...
November 29, 2018: Cellular Physiology and Biochemistry
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"