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https://www.readbyqxmd.com/read/28330267/treatment-of-landfill-leachate-using-asbr-combined-with-zeolite-adsorption-technology
#1
Chi Kim Lim, Ta Wee Seow, Chin Hong Neoh, Muhamad Hanif Md Nor, Zaharah Ibrahim, Ismail Ware, Siti Hajar Mat Sarip
Sanitary landfilling is the most common way to dispose solid urban waste; however, improper landfill management may pose serious environmental threats through discharge of high strength polluted wastewater also known as leachate. The treatment of landfill leachate to fully reduce the negative impact on the environment, is nowadays a challenge. In this study, an aerobic sequencing batch reactor (ASBR) was proposed for the treatment of locally obtained real landfill leachate with initial ammoniacal nitrogen and chemical oxygen demand (COD) concentration of 1800 and 3200 mg/L, respectively...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330257/generation-of-an-inducible-system-to-express-polo-like-kinase-cdc5-as-tap-fusion-protein-during-meiosis-in-saccharomyces-cerevisiae
#2
Rajni Vaid, Kamal Dev, Michael Lichten, Anuradha Sourirajan
Tandem affinity purification (TAP) is a highly efficient method for isolation of protein complexes from endogenous biological macromolecules. TAP system consists of dual affinity tags that facilitates the sequential purification of the desired proteins expressed at their low levels in vivo. Polo-like kinases (PLK) are serine/threonine protein kinases that are the crucial regulators of cell cycle. Cdc5, the solitary PLK in budding yeast Saccharomyces cerevisiae, has diverse array of targets in cell cycle. The present study was undertaken to construct an estrogen-inducible system for expression of Cdc5-TAP to isolate the substrates of Cdc5 during meiosis, particularly, pachytene stage of meiosis I...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330245/co-metabolism-of-thiocyanate-and-free-cyanide-by-exiguobacterium-acetylicum-and-bacillus-marisflavi-under-alkaline-conditions
#3
Lukhanyo Mekuto, Oluwadara Oluwaseun Alegbeleye, Seteno Karabo Obed Ntwampe, Maxwell Mewa Ngongang, John Baptist Mudumbi, Enoch A Akinpelu
The continuous discharge of cyanide-containing effluents to the environment has necessitated for the development of environmentally benign treatment processes that would result in complete detoxification of the cyanide-containing wastewaters, without producing additional environmental toxicants. Since biological detoxification of hazardous chemical compounds has been renowned for its robustness and environmental-friendliness, the ability of the Exiguobacterium acetylicum (GenBank accession number KT282229) and Bacillus marisflavi (GenBank accession number KR016603) to co-metabolise thiocyanate (SCN(-)) and free cyanide (CN(-)) under alkaline conditions was evaluated...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330182/efficient-single-step-chromatographic-purification-of-recombinant-human-antithrombin-rhat-from-saccharomyces-cerevisiae
#4
Maheswara Reddy Mallu, Sandeep Vemula, Srinivasa Reddy Ronda
Antithrombin (AT) is a glycoprotein that inactivates the several physiological target enzymes of coagulation system. The effect of purification strategies plays a crucial role in getting maximum recovery of yield, purity and biological activity of recombinant human antithrombin (rhAT). In the present work, the task of purifying rhAT from Saccharomyces cerevisiae BY4741 has been carried out using two different approaches such as cross flow filtration (CFF) system and chromatography methods. In the first approach, the protein was concentrated and partially purified through CFF to achieve maximum recovery yield and purity of 87 and 94 %, respectively...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330111/effect-of-stat3-inhibitor-in-chronic-myeloid-leukemia-associated-signaling-pathway-a-mathematical-modeling-simulation-and-systems-biology-study
#5
Himansu Kumar, Swapnil Tichkule, Utkarsh Raj, Saurabh Gupta, Swati Srivastava, Pritish Kumar Varadwaj
Chronic myeloid leukemia (CML) is a hematopoietic stem-cell disorder which proliferates due to abnormal growth of basophil cells. Several proangiogenic molecules have been reported to be associated in CML progression, including the hepatocyte growth factor (HGF). However, detail mechanism about the cellular distribution and function of HGF in CML is yet to be revealed. The proliferation of hematopoietic cells are regulated by some of the growth factors like interleukin 3 (IL-3), IL-6, erythropoietin, thrombopoietin, etc...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330085/bioprospecting-and-biotechnological-applications-of-fungal-laccase
#6
REVIEW
Pooja Upadhyay, Rahul Shrivastava, Pavan Kumar Agrawal
Laccase belongs to a small group of enzymes called the blue multicopper oxidases, having the potential ability of oxidation. It belongs to enzymes, which have innate properties of reactive radical production, but its utilization in many fields has been ignored because of its unavailability in the commercial field. There are diverse sources of laccase producing organisms like bacteria, fungi and plants. In fungi, laccase is present in Ascomycetes, Deuteromycetes, Basidiomycetes and is particularly abundant in many white-rot fungi that degrade lignin...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330074/catalytic-properties-functional-attributes-and-industrial-applications-of-%C3%AE-glucosidases
#7
Gopal Singh, A K Verma, Vinod Kumar
β-Glucosidases are diverse group of enzymes with great functional importance to biological systems. These are grouped in multiple glycoside hydrolase families based on their catalytic and sequence characteristics. Most studies carried out on β-glucosidases are focused on their industrial applications rather than their endogenous function in the target organisms. β-Glucosidases performed many functions in bacteria as they are components of large complexes called cellulosomes and are responsible for the hydrolysis of short chain oligosaccharides and cellobiose...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329028/non-equilibrium-assembly-of-microtubules-from-molecules-to-autonomous-chemical-robots
#8
REVIEW
H Hess, Jennifer L Ross
Biological systems have evolved to harness non-equilibrium processes from the molecular to the macro scale. It is currently a grand challenge of chemistry, materials science, and engineering to understand and mimic biological systems that have the ability to autonomously sense stimuli, process these inputs, and respond by performing mechanical work. New chemical systems are responding to the challenge and form the basis for future responsive, adaptive, and active materials. In this article, we describe a particular biochemical-biomechanical network based on the microtubule cytoskeletal filament - itself a non-equilibrium chemical system...
March 22, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28328998/combinatorial-selection-for-replicable-rna-by-q%C3%AE-replicase-while-maintaining-encoded-gene-function
#9
Mio Yumura, Natsuko Yamamoto, Katsushi Yokoyama, Hirotada Mori, Tetsuya Yomo, Norikazu Ichihashi
Construction of a complex artificial self-replication system is challenging in the field of in vitro synthetic biology. Recently, we developed a translation-coupled RNA replication system, wherein an artificial genomic RNA replicates with the Qβ RNA replicase gene encoded on itself. The challenge is to introduce additional genes into the RNA to develop a complex system that mimics natural living systems. However, most RNA sequence encoding genes are not replicable by the Qβ replicase owing to its requirement for strong secondary structures throughout the RNA sequence that are absent in most genes...
2017: PloS One
https://www.readbyqxmd.com/read/28328542/an-excited-state-intramolecular-photon-transfer-fluorescence-probe-for-localizable-live-cell-imaging-of-cysteine
#10
Wei Liu, Wen Chen, Si-Jia Liu, Jian-Hui Jiang
Small molecule probes suitable for selective and specific fluorescence imaging of some important but low-concentration intracellular reactive sulfur species such as cysteine (Cys) pose a challenge in chemical biology. We present a readily available, fast-response fluorescence probe CHCQ-Ac, with 2-(5'-chloro-2-hydroxyl-phenyl)-6-chloro-4(3 H)-quinazolinone (CHCQ) as the fluorophore and acrylate group as the functional moiety, that enables high-selectivity and high-sensitivity for detecting Cys in both solution and biological system...
March 22, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28328541/molecular-engineering-of-two-photon-fluorescent-probes-for-bioimaging-applications
#11
Hong-Wen Liu, Yongchao Liu, Peng Wang, Xiao-Bing Zhang
During the past two decades, two-photon microscopy (TPM), which utilizes two near-infrared photons as the excitation source, has emerged as a novel, attractive imaging tool for biological research. Compared with one-photon microscopy, TPM offers several advantages, such as lowering background fluorescence in living cells and tissues, reducing photodamage to biosamples, and a photobleaching phenomenon, offering better 3D spatial localization, and increasing penetration depth. Small-molecule-based two-photon fluorescent probes have been well developed for the detection and imaging of various analytes in biological systems...
March 22, 2017: Methods and Applications in Fluorescence
https://www.readbyqxmd.com/read/28328511/wireless-power-transfer-strategies-for-implantable-bioelectronics-methodological-review
#12
Kush Agarwal, Rangarajan Jegadeesan, Yong-Xin Guo, Nitish V Thakor
Neural implants have emerged over the last decade as highly effective solutions for the treatment of dysfunctions and disorders of the nervous system. These implants establish a direct, often bidirectional, interface to the nervous system, both sensing neural signals and providing therapeutic treatments. As a result of the technological progress and successful clinical demonstrations, completely implantable solutions have become a reality and are now commercially available for the treatment of various functional disorders...
March 16, 2017: IEEE Reviews in Biomedical Engineering
https://www.readbyqxmd.com/read/28328228/reversible-morphological-control-of-cholecystokinin-tetrapeptide-amyloid-assemblies-as-a-function-of-ph
#13
Frédéric Gobeaux, Florence Porcher, Rajeev Dattani
Most amyloid assemblies are seen as irreversible and exhibit polymorphism because their assembly is kinetically controlled and different structures are trapped during the aggregation process. However, in the specific case of peptide hormones, formation of amyloid assemblies for storage purposes has been reported. This suggests a strict control of assembly and the ability to disassemble upon hormone secretion. In the present work, we have sought to test these assertions with a short peptide, the cholecystokinin (or gastrin) tetrapeptide (CCK-4), that has been found in both gastrointestinal tract and central nervous system, and whose sequence is shared by a large number of hormones...
March 22, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28327938/targeting-the-fibroblast-growth-factor-receptor-2-in-gastric-cancer-promise-or-pitfall
#14
C Hierro, M Alsina, M Sánchez, V Serra, J Rodon, J Tabernero
Gastric cancer is the third leading cause of death from cancer worldwide. Systemic chemotherapy remains the mainstay therapeutic option for this poor prognosis cancer. Trastuzumab, the epidermal growth factor receptor 2 (ERBB2 or HER2)-antibody, is the only biological agent approved for the molecularly-selected population of HER2-positive gastric cancer patients. Over the last decade, several groups have been working for deepening into the molecular characterization of gastric cancer, shedding some light into the heterogeneity of this tumour...
March 7, 2017: Annals of Oncology: Official Journal of the European Society for Medical Oncology
https://www.readbyqxmd.com/read/28327890/draft-genome-of-the-honey-bee-ectoparasitic-mite-tropilaelaps-mercedesae-is-shaped-by-the-parasitic-life-history
#15
Xiaofeng Dong, Stuart D Armstrong, Dong Xia, Benjamin L Makepeace, Alistair C Darby, Tatsuhiko Kadowaki
Background: The number of managed honey bee colonies has considerably decreased in many developed countries in recent years and ectoparasitic mites are considered as major threats to honey bee colonies and health. However, their general biology remains poorly understood. Results: We sequenced the genome of Tropilaelaps mercedesae , the prevalent ectoparasitic mite infesting honey bees in Asia and predicted 15,190 protein-coding genes which were well supported by the mite transcriptomes and proteomic data...
February 22, 2017: GigaScience
https://www.readbyqxmd.com/read/28327791/a-tale-of-two-neglected-tropical-infections-using-gis-to-assess-the-spatial-and-temporal-overlap-of-schistosomiasis-and-leprosy-in-a-region-of-minas-gerais-brazil
#16
David Alexander Phillips, José Antonio Ferreira, Deidra Ansah, Herica Sa Teixeira, Uriel Kitron, Thelma de Filippis, Marcelo H de Alcântara, Jessica K Fairley
BACKGROUND: Despite public health efforts to reduce the global burden of leprosy, gaps remain in the knowledge surrounding transmission of infection. Helminth co-infections have been associated with a shift towards the lepromatous end of the disease spectrum, potentially increasing transmission in co-endemic areas. OBJECTIVES: Using this biologically plausible association, we conducted a geographic information systems (GIS) study to investigate the spatial associations of schistosomiasis and leprosy in an endemic area of Minas Gerais (MG), Brazil...
April 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28327660/quantitative-volumetric-raman-imaging-of-three-dimensional-cell-cultures
#17
Charalambos Kallepitis, Mads S Bergholt, Manuel M Mazo, Vincent Leonardo, Stacey C Skaalure, Stephanie A Maynard, Molly M Stevens
The ability to simultaneously image multiple biomolecules in biologically relevant three-dimensional (3D) cell culture environments would contribute greatly to the understanding of complex cellular mechanisms and cell-material interactions. Here, we present a computational framework for label-free quantitative volumetric Raman imaging (qVRI). We apply qVRI to a selection of biological systems: human pluripotent stem cells with their cardiac derivatives, monocytes and monocyte-derived macrophages in conventional cell culture systems and mesenchymal stem cells inside biomimetic hydrogels that supplied a 3D cell culture environment...
March 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28327522/isolation-and-characterization-of-a-shewanella-phage-host-system-from-the-gut-of-the-tunicate-ciona-intestinalis
#18
Brittany Leigh, Charlotte Karrer, John P Cannon, Mya Breitbart, Larry J Dishaw
Outnumbering all other biological entities on earth, bacteriophages (phages) play critical roles in structuring microbial communities through bacterial infection and subsequent lysis, as well as through horizontal gene transfer. While numerous studies have examined the effects of phages on free-living bacterial cells, much less is known regarding the role of phage infection in host-associated biofilms, which help to stabilize adherent microbial communities. Here we report the cultivation and characterization of a novel strain of Shewanella fidelis from the gut of the marine tunicate Ciona intestinalis, inducible prophages from the S...
March 22, 2017: Viruses
https://www.readbyqxmd.com/read/28327460/comprehensive-proteomic-characterization-of-stem-cell-derived-extracellular-matrices
#19
Héloïse Ragelle, Alexandra Naba, Benjamin L Larson, Fangheng Zhou, Miralem Prijić, Charles A Whittaker, Amanda Del Rosario, Robert Langer, Richard O Hynes, Daniel G Anderson
In the stem-cell niche, the extracellular matrix (ECM) serves as a structural support that additionally provides stem cells with signals that contribute to the regulation of stem-cell function, via reciprocal interactions between cells and components of the ECM. Recently, cell-derived ECMs have emerged as in vitro cell culture substrates to better recapitulate the native stem-cell microenvironment outside the body. Significant changes in cell number, morphology and function have been observed when mesenchymal stem cells (MSC) were cultured on ECM substrates as compared to standard tissue-culture polystyrene (TCPS)...
March 7, 2017: Biomaterials
https://www.readbyqxmd.com/read/28326768/air-pollution-and-climate-change-effects-on-allergies-in-the-anthropocene-abundance-interaction-and-modification-of-allergens-and-adjuvants
#20
Kathrin Reinmuth-Selzle, Christopher J Kampf, Kurt Lucas, Naama Lang-Yona, Janine Fröhlich-Nowoisky, Manabu Shiraiwa, Pascale Sylvie Jeanne Lakey, Senchao Lai, Fobang Liu, Anna Theresa Kunert, Kira Ziegler, Fangxia Shen, Rossella Sgarbanti, Bettina Weber, Iris Bellinghausen, Joachim Saloga, Michael G Weller, Albert Duschl, Detlef Schuppan, Ulrich Pöschl
Air pollution and climate change are potential drivers for the increasing burden of allergic diseases. The molecular mechanisms by which air pollutants and climate parameters may influence allergic diseases, however, are complex and elusive. This article provides an overview of physical, chemical and biological interactions between air pollution, climate change, allergens, adjuvants and the immune system, addressing how these interactions may promote the development of allergies. We reviewed and synthesized key findings from atmospheric, climate, and biomedical research...
March 22, 2017: Environmental Science & Technology
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