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Uri Weill, Gat Krieger, Zohar Avihou, Ron Milo, Maya Schuldiner, Dan Davidi
While protein tags are ubiquitously utilized in molecular biology, they harbor the potential to interfere with functional traits of their fusion counterparts. Systematic evaluation of the effect of protein tags on function would promote accurate use of tags in experimental setups. Here we examine the effect of Green Fluorescent Protein (GFP) tagging at either the N or C terminus of budding yeast proteins on subcellular localization and functionality. We use a competition-based approach to decipher the relative fitness of two strains tagged on the same protein but on opposite termini and from that infer the correct, physiological localization for each protein and the optimal position for tagging...
December 12, 2018: Journal of Molecular Biology
Nele Brusselaers, Sadeep Shrestha, Janneke Van De Wijgert, Hans Verstraelen
OBJECTIVE: The vaginal microbiota proposedly influence the association between human papillomavirus and cervical cancer. Our aim was to assess if vaginal dysbiosis affects human papilloma virus acquistion, persistence, and progression to related cervical pre-malignancy. DATA SORUCES: MEDLINE, Embase, CINAHL, Cochrane Library, and Web of Science (inception until June 2018). The study protocol was registered at PROSPERO (CRD42016035620). STUDY ELIGIBILITY CRITERIA: This systematic review included all observational studies reporting on incident human papilloma virus, persistent human papilloma virus and/or related cervical disease, in women with or without vaginal dysbiosis prior to outcome assessment...
December 11, 2018: American Journal of Obstetrics and Gynecology
Raigam J Martinez-Portilla, Montse Pauta, Ameth Hawkins-Villarreal, Marta Rial-Crestelo, Fernanda Paz Y Miño, Irene Madrigal, Francesc Figueras, Antoni Borrell
OBJECTIVE: To establish the added value of chromosomal microarray analysis (CMA) above normal karyotyping to assess the genetic causes of stillbirth. METHODS: A systematic search was performed to identify relevant studies published in English and Spanish using PubMed, SCOPUS and ISI Web of Knowledge databases, The Cochrane Library and PROSPERO database for meta-analysis, without publication time restrictions. Case-series of fetal losses ≥ 20 weeks of gestation, assessed by CMA with at least 5 subjects analyzed...
December 14, 2018: Ultrasound in Obstetrics & Gynecology
Alessandro Sannino, Elena Gabriele, Martina Bigatti, Sara Mulatto, Jacopo Piazzi, Jörg Scheuermann, Dario Neri, Etienne J Donckele, Florent Samain
DNA-Encoded chemical libraries are often used for the discovery of ligands against protein targets of interest. These large collections of DNA-barcoded chemical compounds are typically screened by using affinity capture methodologies followed by PCR amplification and DNA sequencing procedures. However, the performance of individual steps in the selection procedures has been scarcely investigated so far. Here, we describe a quantitative analysis of selection experiments, using three ligands with different affinity to carbonic anhydrase IX as model compounds...
December 13, 2018: Chembiochem: a European Journal of Chemical Biology
Dirk Stengel, Johannes Leisterer, Paula Ferrada, Axel Ekkernkamp, Sven Mutze, Alexander Hoenning
BACKGROUND: Point-of-care sonography (POCS) has emerged as the screening modality of choice for suspected body trauma in many emergency departments worldwide. Its best known application is FAST (focused abdominal sonography for trauma). The technology is almost ubiquitously available, can be performed during resuscitation, and does not expose patients or staff to radiation. While many authors have stressed the high specificity of POCS, its sensitivity varied markedly across studies. This review aimed to compile the current best evidence about the diagnostic accuracy of POCS imaging protocols in the setting of blunt thoracoabdominal trauma...
December 12, 2018: Cochrane Database of Systematic Reviews
Anne Rodrigues Ferreira, Eliete Farias Azevedo
PROBLEM IDENTIFICATION: Subcutaneous (SC) formulations for monoclonal antibodies (mAbs) must be evaluated for efficacy and safety in comparison with preexisting IV formulations to identify potential benefits and risks. LITERATURE SEARCH: This is a systematic review of clinical trials. MEDLINE®/PubMed, EMBASE, Cochrane Library, LILACS (Latin American and Caribbean Health Sciences Literature), and reference lists were searched for relevant studies. DATA EVALUATION: Data regarding efficacy and safety were registered in a form designed for this review...
January 13, 2019: Oncology Nursing Forum
Mohammed Maqbali, Ciara Hughes, Lynn Dunwoody, Jane P Rankin, Eileen D Hacker, Jackie Gracey
PROBLEM IDENTIFICATION: Fatigue has a negative impact on the quality of life of patients with cancer. The aim of this review is to evaluate studies on the effectiveness of exercise interventions in reducing fatigue in women with gynecologic cancer. LITERATURE SEARCH: The review was conducted according to the PRISMA guidelines using the CINAHL®, MEDLINE®, EMBASE, PsycINFO®, and Cochrane Library databases. The Critical Appraisal Skills Programme was used for quality assessment...
January 13, 2019: Oncology Nursing Forum
Katharina Winiker, Anna Gillman, Esther Guiu Hernandez, Maggie-Lee Huckabee, Kristin Gozdzikowska
PURPOSE: This systematic review appraises and summaries methodology documented in studies using high resolution pharyngeal manometry (HRM) with and without impedance technology (HRIM) in adult populations. METHODS: Four electronic databases CINAHL, EMBASE, MEDLINE, and Cochrane Library were searched up to, and including March 2017. Studies reporting pharyngeal HRM/HRIM for swallowing and/or phonatory assessment, published in peer-reviewed journals in English, German, or Spanish were assessed for the inclusion criteria...
December 13, 2018: European Archives of Oto-rhino-laryngology
Idalia M Yabe, Lauren L Truitt, Diego A Espinoza, Chuanfeng Wu, Samson Koelle, Sandhya Panch, Marcus A F Corat, Thomas Winkler, Kyung-Rok Yu, So Gun Hong, Aylin Bonifacino, Allen Krouse, Mark Metzger, Robert E Donahue, Cynthia E Dunbar
Gene therapies using integrating retrovirus vectors to modify hematopoietic stem and progenitor cells have shown great promise for the treatment of immune system and hematologic diseases. However, activation of proto-oncogenes via insertional mutagenesis has resulted in the development of leukemia. We have utilized cellular bar coding to investigate the impact of different vector designs on the clonal behavior of hematopoietic stem and progenitor cells (HSPCs) during in vivo expansion, as a quantitative surrogate assay for genotoxicity in a non-human primate model with high relevance for human biology...
December 14, 2018: Molecular Therapy. Methods & Clinical Development
Yongbo Wang, Yi Sun, Juan Tang, Wei Zhou, Xiaoxue Liu, Yongyi Bi, Zhi-Jiang Zhang
PURPOSE: Increasing epidemiologic evidence suggests that diabetes mellitus (DM) may be associated with a decreased risk of glioma. This systematic review assessed whether DM was associated with glioma risk. METHODS: Electronic searches were performed in PubMed, Web of Science, EMBASE, and Cochrane Library databases up to August 30, 2018. A random-effects model was performed to calculate summary effect size with corresponding 95% confidence intervals (CIs). RESULTS: In total, 10 studies (eight case-control studies and two cohort studies) matched the inclusion criteria...
November 26, 2018: Annals of Epidemiology
Cristina Olaverri-Monreal, Javier Errea-Moreno, Alberto Díaz-Álvarez, Carlos Biurrun-Quel, Luis Serrano-Arriezu, Markus Kuba
In-vehicle applications that are based on Vehicle-to-Everything (V2X) communication technologies need to be evaluated under lab-controlled conditions before performing field tests. The need for a tailored platform to perform specific research on the cooperative Advanced Driving Assistance System (ADAS) to assess the effect on driver behavior and driving performance motivated the development of a driver-centric traffic simulator that is built over a 3D graphics engine. The engine creates a driving situation as it communicates with a traffic simulator as a means to simulate real-life traffic scenarios...
December 12, 2018: Sensors
Nway Mon Kyaw Soe, Yelena Bird, Michael Schwandt, John Moraros
The purpose of this systematic review and meta-analysis was to address disparities related to sexual health among students by examining the effectiveness of sexually transmitted infection (STI) preventive interventions in educational settings. PubMed, Medline, Cochrane Library, Public Health Database, and EMBASE databases were used to conduct searches. Information relating to studies, programs, participants, and quantitative outcome variables were extracted. Risk of bias was assessed and meta-analysis was conducted...
December 11, 2018: International Journal of Environmental Research and Public Health
Chao Ma, Xing-Guo Nie, Yan-Li Wang, Da-Peng Wu, Qiu-Dong Liang
BACKGROUND: Plasmacytoma variant translocation 1 (PVT1) is reported to be dysregulated in various cancers. Therefore, this meta-analysis was performed to clarify its utility as a prognosis marker in malignant tumors. METHODS: Electronic databases, including PubMed, OVID, Cochrane Library, and Web of Science databases, were retrieved from inception to December 16, 2017. Typically, hazard ratios (HRs) and corresponding 95% confidence intervals (CIs) were calculated, so as to explore the relationship between PVT1 expression and patient survival...
December 2018: Medicine (Baltimore)
Qixin Cao, Yun Wang, Xiaohui Song, Weihua Yang
Retinoblastoma (Rb) is the most common intra-ocular malignancy in children. The association of rs2279744, and rs937283 in MDM2 gene, and p21 rs1801270 polymorphism and RB development have been demonstrated. To provide a comprehensive assessment of and to clarify associations between the 3 SNPs (MDM2 rs2279744, MDM2 rs937283, and p21 rs1801270) and the risk of RB, we performed a meta-analysis of all the eligible case-control studies. We searched English databases include PubMed, Embase, Google Scholar, and Cochrane Library, using an upper date limit of January 1, 2018...
December 2018: Medicine (Baltimore)
Xiaotao Jiang, Linling Xie, Cihui Huang, Yishen Liu, Haining Liu, Binqian Liu, Liang Zheng
BACKGROUND: Characterized by diffuse hepatic fibrosis and nodule formation, hepatitis B cirrhosis (HBC), an important result of chronic hepatitis B development, mainly contains compensated and decompensated stage. Compensated cirrhosis can further develop into decompensated stage and hepatocellular carcinoma with serious complications and high mortality. Antiviral therapy using interferon (IFN) or nucleos(t)ide analogs (NUCs) is essential for improving the prognosis of the disease but IFN has large side effects while NUCs often develop drug resistance...
December 2018: Medicine (Baltimore)
Yu Fan, Xuyu Gu, Heng Zhang
BACKGROUND: Dazhu Hongjingtian (DZHJT), also named Herba Rhodiolae, has been frequently introduced for patients with angina pectoris in China. However, the add-on effect of DZHJT in unstable angina pectoris (UAP) has not been systematically evaluated. The purpose of this protocol is to provide the methods used to assess the efficacy and safety of DZHJT as adjuvant therapy for management of UAP. METHODS: We will extensively search for eligible studies in PubMed, Emase, Cochrane Library, Chinese Biomedical Literature Database, China National Knowledge Infrastructure, Wanfang, and VIP databases up to October 2018...
December 2018: Medicine (Baltimore)
Kun Jin, Linghui Deng, Shi Qiu, Xiang Tu, Jiakun Li, Yige Bao, Lu Yang, Qiang Wei
BACKGROUND: We performed the network meta-analysis (NMA) and systematic review involved all evidence from relevant trials to compare the efficiency and safety of various types of selective serotonin reuptake inhibitors (SSRI) and phosphodiesterase-5 inhibitors (PDE5i) in patients with premature ejaculation (PE). METHODS: We conducted comprehensive searches of peer-reviewed and grey literature. PubMed, the Cochrane Library Central Register of Controlled Trials, Embase were searched for randomized controlled trials published up to June 1, 2017...
December 2018: Medicine (Baltimore)
Junwu Duan, Linlin Pan, Ming Yang
BACKGROUND: Preoperative neutrophil-lymphocyte ratio (NLR) and derived NLR (dNLR) have been suggested to be correlated with the prognosis of patients with breast cancer (BC). However, the results still remain controversial. Therefore, this study was to further evaluate the prognostic potential of preoperative NLR and dNLR for BC patients using a meta-analysis. METHODS: Relevant articles were sought in PubMed and Cochrane Library databases up to September 2018. The associations between preoperative NLR/dNLR and overall survival (OS), disease-free survival (DFS) and recurrence-free survival (RFS) were assessed by the STATA software with the results presented as pooled hazard ratio (HR) with 95% confidence interval (CI)...
December 2018: Medicine (Baltimore)
Qinghua Liu, Jian Cheng, Jingjing Li, Xiang Gao, Hongbo Li
BACKGROUND: Contrast-enhanced ultrasound (CEUS) is a non-invasive method that has been used in the diagnosis of several diseases. Recently, CEUS has been used in the differentiation of benign and malignant thyroid nodules. However, the performance of CEUS in thyroid nodules has not been studied clearly. METHODS: The databases of Pubmed, Embase, Cochrane library and the unpublished studies were systematically searched for candidate inclusions, with the use of CEUS in differentiating the benign and malignant thyroid nodules...
December 2018: Medicine (Baltimore)
Xinwei Zou, Yi Zhang, Lin Zhang, Jiaxi Li, Chenjie Zhu, Qiuhong Cheng, Jinhua Zhou, Youguo Chen
BACKGROUND: Murine double minute 2 homolog (MDM2) plays an important role in the downregulation of P53 tumor suppressor gene. MDM2 inhibits P53 transcriptional activity and thereby results in accelerated tumor formation. Overexpression of MDM2 has been found in several cancer types including endometrial cancer. SNP309 is located in the promoter region of MDM2 and contributes to the overexpression of MDM2. The association between MDM2 SNP309 polymorphism and endometrial cancer risk has been investigated in several studies; however, the conclusion remains controversial...
December 2018: Medicine (Baltimore)
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