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JOURNAL ARTICLE
Na Yan, Zhiwei Hu, Lingling Zhang
The development of a bio-sensing strategy based on CRISPR/Cas that is exceptionally sensitive is crucial for the identification of trace molecules. Colorimetric miRNA detection utilizing CRISPR/Cas13a-triggered DNAzyme signal amplification was described in this article. The developed strategy was implemented for miRNA-21 detection as a proof of concept. The cleavage activity of Cas13a was triggered when the target molecule bonded to the Cas13a-crRNA complex and cleaved uracil ribonucleotides (rU) in the substrate probe...
April 23, 2024: Applied Biochemistry and Biotechnology
#2
REVIEW
Ocílio Ribeiro Gonçalves, Vitor E A Ribeiro, Maria T L Galvão, Raquel Oliveira de Sousa Silva, Milene V S Sobral, Giovana S A Kojima, João L R Freitas, Victor G Soares, Benjamim B de Azevedo, Caroline Baima de Melo
Certain guidelines recommend a second-generation H1-antihistamine (AH) as first-line treatment for patients with chronic urticaria (CU). However, some patients show insufficient response to a standard dose of this therapy and might benefit from adding leukotriene receptor antagonists (LTA). Therefore, we aimed to perform a systematic review and meta-analysis comparing LTA plus antihistamines with antihistamines alone. We performed a systematic review and meta-analysis, searching PubMed, EMBASE, and Cochrane Central for randomized clinical trial (RCT) data comparing LTA plus AH treatment to AH alone in patients with CU...
April 22, 2024: International Journal of Dermatology
#3
JOURNAL ARTICLE
Yuying Han, Zhenghong Yuan, Zhigang Yi
The coronavirus disease 2019 (COVID-19) pandemic, caused by the novel coronavirus severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), has rapidly spread worldwide since its emergence in late 2019. Its ongoing evolution poses challenges for antiviral drug development. Coronavirus nsp6, a multiple-spanning transmembrane protein, participates in the biogenesis of the viral replication complex, which accommodates the viral replication-transcription complex. The roles of its structural domains in viral replication are not well studied...
April 19, 2024: Journal of Virology
#4
JOURNAL ARTICLE
Fan Yang, Luyao Zhang, Minghui Yang, Jianfeng Chen, Wenzhong Xing
OBJECT: This study aimed to investigate the changes in the translucency and color of four different multi-layered zirconia materials when the sintering temperature were inaccurate. MATERIALS AND METHODS: Two hundred zirconia samples (11 × 11 × 1.0 mm) of four multi-layered zirconia, Upcera TT-GT (UG), Upcera TT-ML (UM), Cercon xt ML (CX), and Lava Esthetic (LE), were divided into five subgroups according to the sintering temperature: L1 (5% lower temperature), L2 (2...
April 18, 2024: BMC Oral Health
#5
JOURNAL ARTICLE
Z Beau Reneer, Harrison C Bergeron, Stephen Reynolds, Elena Thornhill-Wadolowski, Lan Feng, Marcin Bugno, Agnieszka D Truax, Ralph A Tripp
Influenza viruses cause epidemics and can cause pandemics with substantial morbidity with some mortality every year. Seasonal influenza vaccines have incomplete effectiveness and elicit a narrow antibody response that often does not protect against mutations occurring in influenza viruses. Thus, various vaccine approaches have been investigated to improve safety and efficacy. Here, we evaluate an mRNA influenza vaccine encoding hemagglutinin (HA) proteins in a BALB/c mouse model. The results show that mRNA vaccination elicits neutralizing and serum antibodies to each influenza virus strain contained in the current quadrivalent vaccine that is designed to protect against four different influenza viruses including two influenza A viruses (IAV) and two influenza B (IBV), as well as several antigenically distinct influenza virus strains in both hemagglutination inhibition assay (HAI) and virus neutralization assays...
2024: PloS One
#6
JOURNAL ARTICLE
Rebecca R Valentino, William J Scotton, Shanu F Roemer, Tammaryn Lashley, Michael G Heckman, Maryam Shoai, Alejandro Martinez-Carrasco, Nicole Tamvaka, Ronald L Walton, Matthew C Baker, Hannah L Macpherson, Raquel Real, Alexandra I Soto-Beasley, Kin Mok, Tamas Revesz, Elizabeth A Christopher, Michael DeTure, William W Seeley, Edward B Lee, Matthew P Frosch, Laura Molina-Porcel, Tamar Gefen, Javier Redding-Ochoa, Bernardino Ghetti, Andrew C Robinson, Christopher Kobylecki, James B Rowe, Thomas G Beach, Andrew F Teich, Julia L Keith, Istvan Bodi, Glenda M Halliday, Marla Gearing, Thomas Arzberger, Christopher M Morris, Charles L White, Naguib Mechawar, Susana Boluda, Ian R MacKenzie, Catriona McLean, Matthew D Cykowski, Shih-Hsiu J Wang, Caroline Graff, Rashed M Nagra, Gabor G Kovacs, Giorgio Giaccone, Manuela Neumann, Lee-Cyn Ang, Agostinho Carvalho, Huw R Morris, Rosa Rademakers, John A Hardy, Dennis W Dickson, Jonathan D Rohrer, Owen A Ross
BACKGROUND: Pick's disease is a rare and predominantly sporadic form of frontotemporal dementia that is classified as a primary tauopathy. Pick's disease is pathologically defined by the presence in the frontal and temporal lobes of Pick bodies, composed of hyperphosphorylated, three-repeat tau protein, encoded by the MAPT gene. MAPT has two distinct haplotypes, H1 and H2; the MAPT H1 haplotype is the major genetic risk factor for four-repeat tauopathies (eg, progressive supranuclear palsy and corticobasal degeneration), and the MAPT H2 haplotype is protective for these disorders...
May 2024: Lancet Neurology
#7
JOURNAL ARTICLE
Jose L Martínez, Nicholas Lemus, Tsoi Ying Lai, Mitali Mishra, Irene González-Domínguez, Eduard Puente-Massaguer, Madhumathi Loganathan, Benjamin Francis, Marie I Samanovic, Florian Krammer, Mark J Mulligan, Viviana Simon, Peter Palese, Weina Sun
The head domain of the hemagglutinin of influenza viruses plays a dominant role in the antibody response due to the presence of immunodominant antigenic sites that are the main targets of host neutralizing antibodies. For the H1 hemagglutinin, five major antigenic sites defined as Sa, Sb, Ca1, Ca2, and Cb have been described. Although previous studies have focused on defining the hierarchy of the antigenic sites of the hemagglutinin in different human cohorts, it is still unclear if the immunodominance profile of the antigenic sites might change with the antibody levels of individuals or if other demographic factors (such as exposure history, sex, or age) could also influence the importance of the antigenic sites...
April 15, 2024: Vaccine
#8
JOURNAL ARTICLE
Ya-Chi Tu, Yu-Shan Hsueh, Yu-Chen Cheng, Ting-Wei Lin, Tzong-Shi Chiueh
BACKGROUND AND AIMS: Besides hospital size, clinical diagnosis and severity of patient cases determine the total platelet usage. Therefore, the appropriateness of platelet usage could not be compared simply with the total units of platelet usage in each hospital. This study aimed to objectively monitor and analyze platelet usage after implementing a single-unit issuing policy for each platelet transfusion in our hospital in October 2020. MATERIALS AND METHODS: We used three objective indices, X, Y, and Z, to monitor platelet usage and compared it with other hospitals...
April 2024: Health Science Reports
#9
JOURNAL ARTICLE
Kaili Guo, Yu Jiang, Wei Qiao, Panpan Yuan, Miao Xue, Jiping Liu, Hao Wei, Bin Wang, Xingmei Zhu
The alcohol extraction of P. sibiricum has exhibited significant inhibitory effects on the production of free radicals and the proliferation of non-small-cell lung carcinoma (NSCLC) A549 cells. Despite the diverse components found in alcohol extraction of P. sibiricum and its multiple targets, the active components and associated targets remain largely unidentified. Hence, there is a need for additional investigation into the pharmacodynamic elements and mechanisms of action. This study aimed to analyze and identify the components responsible for the anti-tumor activity of alcohol extraction from P...
April 15, 2024: Heliyon
#10
JOURNAL ARTICLE
Janice Z Jia, Carolyn A Cohen, Haogao Gu, Milla R McLean, Raghavan Varadarajan, Nisha Bhandari, Malik Peiris, Gabriel M Leung, Leo L M Poon, Tim Tsang, Amy W Chung, Benjamin J Cowling, Nancy H L Leung, Sophie A Valkenburg
Cross-reactive antibodies with Fc receptor (FcR) effector functions may mitigate pandemic virus impact in the absence of neutralizing antibodies. In this exploratory study, we use serum from a randomized placebo-controlled trial of seasonal trivalent influenza vaccination in children (NCT00792051) conducted at the onset of the 2009 H1N1 pandemic (pH1N1) and monitored for infection. We found that seasonal vaccination increases pH1N1 specific antibodies and FcR effector functions. Furthermore, prospective baseline antibody profiles after seasonal vaccination, prior to pH1N1 infection, show that unvaccinated uninfected children have elevated ADCC effector function, FcγR3a and FcγR2a binding antibodies to multiple pH1N1 proteins, past seasonal and avian (H5, H7 and H9) strains...
April 13, 2024: Nature Communications
#11
JOURNAL ARTICLE
Huifang Bai, Jing Ding, Saining Wang, Shuyan Zhang, Ning Jiang, Xiaoxia Wu, Guoliang Chen, Qianqian Dang, Mingyuan Liu, Bin Tang, Xuelin Wang
As an intracellular parasitic nematode, Trichinella spiralis (T. spiralis) can induce the formation of nurse cells (NC) in host muscles and keep it to survive within the NC for an extended period. The formation of NC is similar to muscle cell injury and repair which lead to the arrest of satellite cells in the G2/M phase and build a suitable parasitic environment for the muscle larvae of T. spiralis. However, the molecular mechanisms involved in skeletal muscle repair through skeletal muscle satellite cells (SMSC) and the host immune response during T...
April 6, 2024: Veterinary Parasitology
#12
JOURNAL ARTICLE
Yong-Myung Kang, Erdene-Ochir Tseren Ochir, Gyeong-Beom Heo, Se-Hee An, Hwanseok Jeong, Urankhaich Dondog, Temuulen Myagmarsuren, Youn-Jeong Lee, Kwang-Nyeong Lee
The introduction of novel highly pathogenic (HPAI) viruses into Korea has been attributed to recombination events occurring at breeding sites in the Northern Hemisphere. This has increased interest in monitoring and genetically analyzing avian influenza viruses (AIVs) in northern regions, such as Mongolia, which share migratory bird flyways with Korea. AIVs in Mongolia were monitored by analyzing 10,149 fecal samples freshly collected from wild birds from April to October in 2021 to 2023. The prevalence of AIVs in wild birds was 1...
April 4, 2024: Animals: An Open Access Journal From MDPI
#13
JOURNAL ARTICLE
Magdalena Luty-Błocho, Jowita Cyndrowska, Bogdan Rutkowski, Volker Hessel
In this work, UV-Vis spectrophotometry, High Resolution Scanning Transmission Electron Microscopes and selected experimental conditions were used to screen the colloidal system. The obtained results complement the established knowledge regarding the mechanism of nanoparticle formation. The process of gold nanoparticles formation involves a two-step reduction of Au ions to Au(0); atom association and metastable cluster formation; autocatalytic cluster growth; ultra-small particle formation (1-2 nm, in diameter); particle growth and larger particles formation; and further autocatalytic crystal growth (D > 100 nm)...
March 22, 2024: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
#14
JOURNAL ARTICLE
Vanessa Marchetti, Angelo Panno, Massimiliano Scopelliti, Luciano Romano, Giacomo Angelini, Elena Rinallo, Daniela Barni, Caterina Fiorilli
BACKGROUND: Global climate change is recognized as a major and irreversible challenge for humanity, requiring people's responsible and sustainable behaviors toward the environment. So far, the literature has widely investigated the role of cognitive determinants of ecological outcomes (e.g., pro-environmental behaviors and climate change perception), while less attention has been devoted to emotional processes, such as trait emotional intelligence (TEI). The current double study investigates whether TEI is directly and indirectly associated with climate change perception (CCP, Study 1) and pro-environmental behaviors (PEBs, Study 2) among young adults...
April 12, 2024: BMC Psychology
#15
JOURNAL ARTICLE
Masatomo Ishioh, Tsukasa Nozu, Saori Miyagishi, Sho Igarashi, Takuya Funayama, Nobuhiro Ueno, Toshikatsu Okumura
Intestinal barrier dysfunction, leaky gut, is implicated in various diseases, including irritable bowel syndrome (IBS) and neurodegenerative conditions like Alzheimer's disease. Our recent investigation revealed that basal forebrain cholinergic neurons (BFCNs), critical for cognitive function, receive signals from butyrate and orexin, playing a role in regulating intestinal barrier function through adenosine A2B signaling and the vagus. This study explores the involvement and function of brain histamine, linked to BFCNs, in the regulation of intestinal barrier function...
April 10, 2024: Biochemical Pharmacology
#16
JOURNAL ARTICLE
Alexis J Haas, Mert Karakus, Ceniz Zihni, Maria S Balda, Karl Matter
Tight junctions are a barrier-forming cell-cell adhesion complex and have been proposed to regulate cell proliferation. However, the underlying mechanisms are not well understood. Here, we used cells deficient in the junction scaffold ZO-1 alone or together with its paralog ZO-2, which disrupts the junctional barrier. We found that ZO-1 knockout increased cell proliferation, induced loss of cell density-dependent proliferation control, and promoted apoptosis and necrosis. These phenotypes were enhanced by double ZO-1/ZO-2 knockout...
April 5, 2024: Cells
#17
JOURNAL ARTICLE
Xingxing Jian, Guixue Hou, Liqiao Li, Zhuo Diao, Yingfang Wu, Jiayi Wang, Lu Xie, Cong Peng, Liang Lin, Jie Li
BACKGROUND: Population-based studies have highlighted the link between chronic urticaria (CU) and metabolic syndrome, and metabolic alterations have been revealed in CU. However, to our knowledge, a comprehensive metabolomics study on a large cohort of CU patients has not been reported. OBJECTIVE: To explore the underlying metabolic subtypes and novel metabolite biomarkers for CU diagnosis and therapy. METHODS: Plasma samples from 80 CU patients and 82 healthy controls (HCs) were collected for metabolomics quantification and performed bioinformatics analysis...
April 8, 2024: Journal of Allergy and Clinical Immunology
#18
JOURNAL ARTICLE
Jinwen Zhao, Xingrong Tan, Ying He, Ruo Yuan, Shentang Wang, Shihong Chen
Currently reported aggregation-induced electroluminescence (AIECL) is usually based on the electrostatic integration of luminous monomers, and its application is still limited by the low ECL efficiency and poor structural stability of electrostatic integration-based AIECL emitters. Herein, host-guest recognition-mediated supramolecular AIECL was creatively developed to overcome the defects of electrostatic-integration-based AIECL. Cucurbit[8]uril (CB[8]) as the host recognized tris (2-phenylpyridine) iridium(III) [Ir(ppy)3 ] as the guest to form a novel supramolecular complex Ir-CB[8]...
April 10, 2024: Analytical Chemistry
#19
JOURNAL ARTICLE
François de Kermenguy, Nathan Benzazon, Pauline Maury, Rémi Vauclin, Meissane M'hamdi, Vjona Cifliku, Elaine Limkin, Ibrahima Diallo, Daphné Morel, Candice Milewski, Céline Clémenson, Michele Mondini, Eric Deutsch, Charlotte Robert
Severe radiation-induced lymphopenia occurs in 40% of patients treated for primary brain tumors and is an independent risk factor of poor survival outcomes. We developed an in-silico framework that estimates the radiation doses received by lymphocytes during VMAT brain irradiation. 
Approach: We implemented a simulation consisting of two interconnected compartmental models describing the slow recirculation of lymphocytes between lymphoid organs (M_1) and the bloodstream (M_2). We used dosimetry data from 33 patients treated with chemo-radiation for glioblastoma to compare three cases of the model, corresponding to different physical and biological scenarios: H1) lymphocytes circulation only in the bloodstream i...
April 9, 2024: Physics in Medicine and Biology
#20
JOURNAL ARTICLE
Sofia C Orellana, Richard A I Bethlehem, Ivan L Simpson-Kent, Anne-Laura van Harmelen, Petra E Vértes, Edward T Bullmore
Childhood maltreatment (CM) leads to a lifelong susceptibility to mental ill-health which might be reflected by its effects on adult brain structure, perhaps indirectly mediated by its effects on adult metabolic, immune, and psychosocial systems. Indexing these systemic factors via body mass index (BMI), C-reactive protein (CRP), and rates of adult trauma (AT), respectively, we tested three hypotheses: (H1) CM has direct or indirect effects on adult trauma, BMI, and CRP; (H2) adult trauma, BMI, and CRP are all independently related to adult brain structure; and (H3) childhood maltreatment has indirect effects on adult brain structure mediated in parallel by BMI, CRP, and AT...
April 16, 2024: Proceedings of the National Academy of Sciences of the United States of America
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