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Protein ligation

Guodong Zheng, Hong Qu, Fen Li, Weiquan Ma, Hong Yang
Previous studies have indicated that propofol has immunomodulatory and antioxidative properties. However, the renoprotection effect and the precise mechanisms of propofol in sepsis-induced renal injury remain unclear. The purpose of the present study was to investigate the role of miR-290-5p/CCL-2 signaling in septic mice treatment with propofol. Mice were treated with propofol (50 mg/kg) twice within 24 h. Survival outcome was monitored within 48 h. The mRNA and protein levels were assayed by qRT-PCR and western blotting, respectively...
October 11, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
Ying Zhang, Xiaocui Huang, Fang Luo, Yun Lei, Li Chen, Zuquan Weng, Longhua Guo, Zhenyu Lin
Golgi protein 73 (GP73) is a potential hepatocellular carcinoma serum marker with better sensitivity and specificity. In this study, a highly sensitive electrochemical immunosensor which combines the highly selective proximity ligation assay (PLA) and highly efficient enzyme-powered recycling amplification has been developed for GP73 determination. PLA is triggered by affinity binding of two labelled antibody-DNA (P1-RAb and P2-MAb) to target protein, resulting in increased specificity. The formed immunocomplex hybrids with DNA2 to realize the release of methylene blue labelled mononucleotides fragment with the help of exonuclease III, which acts as the power of recycling amplification...
December 21, 2018: Analytica Chimica Acta
Yasuhiro Miki, Erina Iwabuchi, Katsuhiko Ono, Hironobu Sasano, Kiyoshi Ito
Estrogen receptors promote target gene transcription when they form a dimer, in which two identical (homodimer) or different (heterodimer) proteins are bound to each other. In hormone-dependent cancers, hormone receptor dimerization plays pivotal roles, not only in the pathogenesis or development of the tumors, but also in the development of therapeutic resistance. Protein⁻protein interactions (PPIs), including dimerization and complex formation, have been also well-known to be required for proteins to exert their functions...
October 15, 2018: International Journal of Molecular Sciences
Xi Liu, John M Abraham, Yulan Cheng, Zhixiong Wang, Zhe Wang, Guanjun Zhang, Hassan Ashktorab, Duane T Smoot, Robert N Cole, Tatiana N Boronina, Lauren R DeVine, C Conover Talbot, Zhengwen Liu, Stephen J Meltzer
MicroRNA (miR) sponges containing miR binding sequences constitute a potentially powerful molecular therapeutic strategy. Recently, naturally occurring circular RNAs (circRNAs) were shown to function as efficient miR sponges in cancer cells. We hypothesized that synthetic circRNA sponges could achieve therapeutic loss-of-function targeted against specific miRs. Linear RNA molecules containing miR-21 binding sites were transcribed in vitro; after dephosphorylation and phosphorylation, circularization was achieved using 5'-3' end-ligation by T4 RNA ligase 1...
September 22, 2018: Molecular Therapy. Nucleic Acids
Itay Ayalon, Hui Shen, Lauren Williamson, Keith Stringer, Basilia Zingarelli, Jennifer M Kaplan
Severe sepsis and septic shock are the biggest cause of mortality in critically ill patients. Obesity today is one of the world's greatest health challenges. Little is known about the extent of involvement of the white adipose tissue (WAT) in sepsis and how it is being modified by obesity. We sought to explore the involvement of the WAT in sepsis. We hypothesize that sepsis induces browning of the WAT and that obesity alters the response of WAT to sepsis. Six-week-old C57BL/6 mice were randomized to a high-fat diet to induce obesity (obese group) or control diet (nonobese group)...
November 2018: Shock
Dhiraj P Murale, Seong Cheol Hong, Se-Young Jang, Jun-Seok Lee
Many intracellular proteins are metabolically unstable, and their half-life was known to be controlled by so-called "N-end rule" that is N-terminal residue controlling for protein stability. To visualize or measure the cellular stability of a protein depending on the N-terminal residues, it is getting attention to develop selective labeling methods for individual N-terminal amino acid. However, there are only a few limited functional groups available for specific N-terminal amino acid labeling in a biological environment...
October 16, 2018: Chembiochem: a European Journal of Chemical Biology
Lingling Yu, Weifang Zhang, Chahua Huang, Qian Liang, Huihui Bao, Zhijian Gong, Minxuan Xu, Zhenzhen Wang, Minhua Wen, Xiaoshu Cheng
Myocardial cell apoptosis is the main pathophysiological process underlying ischemia-reperfusion (I/R) injury. FoxO4, which was initially identified as a tumor suppressor that limits cell proliferation and induces apoptosis, plays diverse roles in cardiovascular diseases. However, its contribution to myocardial I/R injury remains unclear. The present study was undertaken to explore the role of FoxO4 in apoptosis during myocardial I/R injury and its underlying mechanisms in vivo . Rats were subjected to ligation/restoration of the left anterior descending branch of the coronary artery and 30 min of ischemia, followed by 4 h of reperfusion...
2018: American Journal of Translational Research
Flavien Zannini, Thomas Roret, Jonathan Przybyla-Toscano, Tiphaine Dhalleine, Nicolas Rouhier, Jérémy Couturier
In plants, the mitochondrial thioredoxin (TRX) system generally comprises only one or two isoforms belonging to the TRX h or o classes, being less well developed compared to the numerous isoforms found in chloroplasts. Unlike most other plant species, Arabidopsis thaliana possesses two TRXo isoforms whose physiological functions remain unclear. Here, we performed a structure⁻function analysis to unravel the respective properties of the duplicated TRXo1 and TRXo2 isoforms. Surprisingly, when expressed in Escherichia coli , both recombinant proteins existed in an apo-monomeric form and in a homodimeric iron⁻sulfur (Fe-S) cluster-bridged form...
October 13, 2018: Antioxidants (Basel, Switzerland)
Waqar Imtiaz, Ahrar Khan, Shafia Tehseen Gul, Muhammad Saqib, Muhammad Kashif Saleemi, Asim Shahzad, Jianbao Dong, Riaz Hussain, Meiyan Shen, Xiaoxia Du
Brucellosis is a highly contagious and zoonotic disease and has a considerable impact on animal health and economy of a country, principally in Pakistan, where rural income largely depends upon livestock farming and dairy products. The disease burden is more in underdeveloped/developing countries due to the low economy and limited access to the diagnostic facilities. In Pakistan, the prevalence of Brucella abortus is very high, so it is the need of the hour to control this disease through more advanced methods...
October 12, 2018: Microbial Pathogenesis
Bing Tian, Koa Hosoki, Zhiqing Liu, Jun Yang, Yingxin Zhao, Hong Sun, Jia Zhou, Erik Rytting, Lata Kaphalia, William J Calhoun, Sanjiv Sur, Allan R Brasier
BACKGROUND: Frequent exacerbations of allergic asthma leads to airway remodeling and a decline in pulmonary function, producing morbidity. Cat dander is an aeroallergen associated with asthma risk. OBJECTIVE: We sought to elucidate the mechanism of cat dander-induced inflammation-remodeling. METHODS: We identified remodeling in mucosal samples from allergic asthma by Q-RT-PCR. We developed a model of aeroallergen-induced experimental asthma by repetitive cat dander extract exposure...
October 12, 2018: Journal of Allergy and Clinical Immunology
Xusan Xu, Chunmei Liang, Dong Lv, Jingwen Yin, Xudong Luo, Jiawu Fu, Haifeng Yan, Xia Zhou, Zhun Dai, Dongjian Zhu, Susu Xiong, Zhixiong Lin, Juda Lin, Bin Zhao, You Li, Yajun Wang, Guoda Ma, Keshen Li
The SAP97 gene is located in the schizophrenia susceptibility locus 3q29, and it encodes the synaptic scaffolding protein that interacts with the N-methyl-D-aspartate (NMDA) receptor, which is presumed to be dysregulated in schizophrenia. In this study, we genotyped a single-nucleotide polymorphism (SNP) (rs3915512) in the SAP97 gene in 1114 patients with schizophrenia and 1036 healthy-matched controls in a Han Chinese population through the improved multiplex ligation detection reaction (imLDR) technique. Then, we analyzed the association between this SNP and the patients' clinical symptoms and neurocognitive function...
2018: Frontiers in Psychiatry
Cheng Zheng, Jia-Feng Lin, Zhong-Hao Lin, Wei-Qian Lin, Saroj Thapa, Yuan-Zheng Lin, Hao Lian, Zhi-Rui Liu, Jia-Hui Chen, Xiao-Wei Li
With the chronic ischemia persisting after acute myocardial infarction, the accompanying low-degree inflammation and subsequent fibrosis result in progression of cardiac remodeling and heart failure. Recently, Sodium Houttuyfonate (SH), a pure compound extracted from Houttuynia cordata , has been confirmed exerting anti-inflammatory and anti-fibrotic effects under diseased situations. Here, we aimed to investigate whether SH could reverse the cardiac remodeling post-myocardial infarction by alleviating cardiac inflammation and fibrosis...
2018: Frontiers in Pharmacology
Shuya Mei, Shuang Wang, Shuqing Jin, Xiang Zhao, Zhenzhen Shao, Renlingzi Zhang, Xiangsheng Yu, Yao Tong, Shibiao Chen, Zhixia Chen, Quan Li
Mechanical ventilation (MV) can augment sepsis-induced organ injury. Previous studies indicate that human mesenchymal stem cells (hMSCs) have immune-modulatory effect. We hypothesize that human adipose tissue-derived stromal cells (hADSCs) could attenuate MV and sepsis-induced organ injury. Male C57BL/6 mice were randomized to five groups: Sham group; MV group; cecal ligation and puncture (CLP) group; CLP + MV group; and CLP + MV + hADSC group. Anesthetized mice were subjected to cecal ligation and puncture surgery...
October 13, 2018: Inflammation
Preeti Singh, Pranshu Sharma, Udayraj P Nakade, Abhishek Sharma, Manju Gari, Soumen Choudhury, Amit Shukla, Satish Kumar Garg
BACKGROUND: Endocannabinoids level are reported to increase in sepsis, however, the role of vascular cannabinoid receptor-1 (CB1R) in sepsis-induced vascular hyporeactivity is yet to be unravelled. METHODS: Polymicrobial sepsis was induced by caecal ligation and puncture in mice. Isometric tension in isolated aortic rings during early (6 h) and late (20 h) phases of sepsis was recorded and expression of mRNA of monoacylglycerol lipase (MAGL) and cannabinoid receptor-1 (CB1R) was investigated...
July 19, 2018: Pharmacological Reports: PR
Paul E Pace, Alexander V Peskin, Andreas Konigstorfer, Christine J Jasoni, Christine C Winterbourn, Mark B Hampton
Hydrogen peroxide (H2 O2 ) acts as a signaling molecule in cells by oxidising cysteine residues in regulatory proteins such as phosphatases, kinases and transcription factors. It is unclear exactly how many of these proteins are specifically targeted by H2 O2 because they appear too unreactive to be directly oxidised. One proposal is that peroxiredoxins (Prxs) initially react with H2 O2 and then oxidise adjacent proteins via a thiol relay mechanism. The aim of this study was to identify constitutive interaction partners of Prx2 in Jurkat T-lymphoma cells, in which thiol protein oxidation occurs at low micromolar concentrations of H2 O2 ...
October 10, 2018: Free Radical Biology & Medicine
Lars Magnius, John Taylor, William S Mason, Camille Sureau, Paul Dény, Helene Norder, Ictv Report Consortium
Hepatitis delta virus, the only member of the only species in the genus Deltavirus, is a unique human pathogen. Its ~1.7 kb circular negative-sense RNA genome encodes a protein, hepatitis delta antigen, which occurs in two forms, small and large, both with unique functions. Hepatitis delta virus uses host RNA polymerase II to replicate via double rolling circle RNA synthesis. Newly synthesized linear RNAs are circularized after autocatalytic cleavage and ligation. Hepatitis delta virus requires the envelope of the helper virus, hepatitis B virus (family Hepadnaviridae), to produce infectious particles...
October 12, 2018: Journal of General Virology
Heow Won Lee, Monir Ahmad, Jonathan J Weldrick, Hong-Wei Wang, Patrick G Burgon, Frans H H Leenen
Exercise training is beneficial for preserving cardiac function post myocardial infarction (MI), but the underlying mechanisms are not well understood. We investigated one possible mechanism, brain-derived neurotrophic factor (BDNF)-tropomyosin-related kinase B (TrkB) signaling with the TrkB blocker, ANA-12 (0.5 mg/kg/day). Male Wistar rats underwent sham surgery or ligation of left descending coronary artery. Surviving MI rats were allocated to sedentary MI with vehicle (Sed-MI-Veh), exercise MI with vehicle (ExT-MI-Veh) and exercise MI with ANA-12 (ExT-MI-ANA-12)...
October 12, 2018: American Journal of Physiology. Heart and Circulatory Physiology
Siham Hedir, Marcella De Giorgi, Jade Fogha, Martina De Pascale, Louis-Bastien Weiswald, Emilie Brotin, Bogdan Marekha, Christophe Denoyelle, Camille Denis, Peggy Suzanne, Fabien Gautier, Philippe Juin, Laetitia Ligat, Frédéric Lopez, Ludovic Carlier, Rémi Legay, Ronan Bureau, Sylvain Rault, Laurent Poulain, Jana Sopková-de Oliveira Santos, Anne Sophie Voisin-Chiret
Protein-protein interactions are attractive targets because they control numerous cellular processes. In oncology, apoptosis regulating Bcl-2 family proteins are of particular interest. Apoptotic cell death is controlled via PPIs between the anti-apoptotic proteins hydrophobic groove and the pro-apoptotic proteins BH3 domain. In ovarian carcinoma, it has been previously demonstrated that Bcl-xL and Mcl-1 cooperate to protect tumor cells against apoptosis. Moreover, Mcl-1 is a key regulator of cancer cell survival and is a known resistance factor to Bcl-2/Bcl-xL pharmacological inhibitors making it an attractive therapeutic target...
November 5, 2018: European Journal of Medicinal Chemistry
Juan Bautista Blanco-Canosa, Judith Palà-Pujadas, Fernando Albericio
A simple procedure for C-terminal activation of peptides in solution and its application in native chemical ligation and protein synthesis is described. This method involves a mild thioesterification based on the conversion of an aryloxy-o-methylaminoanilide to thioester under aqueous conditions and in situ ligation with an N-terminal cysteine peptide. The versatility is shown in ph-controlled sequential ligations. To illustrate the usefulness of this methodology, we synthesized the palmitoylated N-terminal domain of human Sonic Hedgehog, a morphogen protein that binds the transmembrane receptor Patched and activates the Hedgehog signaling pathway, involved in embryonic development and in the proliferation of many tumors...
October 11, 2018: Angewandte Chemie
Angela Raucci, Stefania Di Maggio, Francesco Scavello, Alessandro D'Ambrosio, Marco E Bianchi, Maurizio C Capogrossi
High mobility group box 1 (HMGB1) is a ubiquitous nuclear protein involved in transcription regulation, DNA replication and repair and nucleosome assembly. HMGB1 is passively released by necrotic tissues or actively secreted by stressed cells. Extracellular HMGB1 acts as a damage-associated molecular pattern (DAMPs) molecule and gives rise to several redox forms that by binding to different receptors and interactors promote a variety of cellular responses, including tissue inflammation or regeneration. Inhibition of extracellular HMGB1 in experimental models of myocardial ischemia/reperfusion injury, myocarditis, cardiomyopathies induced by mechanical stress, diabetes, bacterial infection or chemotherapeutic drugs reduces inflammation and is protective...
October 10, 2018: Cellular and Molecular Life Sciences: CMLS
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