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https://www.readbyqxmd.com/read/30313613/a-new-species-of-cascade-frog-amphibia-ranidae-in-the-amolops-monticola-group-from-china
#1
Zhiyong Yuan, Jieqiong Jin, Jiannan Li, Bryan L Stuart, Jun Wu
A new species, Amolops wenshanensis sp. nov., is described from Guangxi and Yunnan Provinces, China. The new species is a member of the A. monticola group, and is distinguished from its congeners using morphological and molecular data. The new species is diagnosed by having glandular dorsolateral folds; smooth skin; side of head dark with a light-colored upper lip stripe extending to axilla; green dorsal coloration in life; immaculate venter; indistinct transverse bands on dorsal surfaces of limbs; tympanum distinct; pineal body absent; all fingertips expanded with circummarginal grooves; two oblique vomerine teeth; vocal sac and white nuptial pad present in males; supratympanic fold absent; outer metatarsal tubercle absent; glandular gold-white flank spot absent; and skin on venter not translucent...
May 1, 2018: Zootaxa
https://www.readbyqxmd.com/read/30271904/anisogamy-evolved-with-a-reduced-sex-determining-region-in-volvocine-green-algae
#2
Takashi Hamaji, Hiroko Kawai-Toyooka, Haruka Uchimura, Masahiro Suzuki, Hideki Noguchi, Yohei Minakuchi, Atsushi Toyoda, Asao Fujiyama, Shin-Ya Miyagishima, James G Umen, Hisayoshi Nozaki
Male and female gametes differing in size-anisogamy-emerged independently from isogamous ancestors in various eukaryotic lineages, although genetic bases of this emergence are still unknown. Volvocine green algae are a model lineage for investigating the transition from isogamy to anisogamy. Here we focus on two closely related volvocine genera that bracket this transition-isogamous Yamagishiella and anisogamous Eudorina . We generated de novo nuclear genome assemblies of both sexes of Yamagishiella and Eudorina to identify the dimorphic sex-determining chromosomal region or mating-type locus ( MT ) from each...
2018: Communications biology
https://www.readbyqxmd.com/read/30053740/de-novo-assembly-and-comparative-transcriptome-analysis-reveals-genes-potentially-involved-in-tissue-color-changes-in-centipedegrass-eremochloa-ophiuroides-munro-hack
#3
Jianjian Li, Junqin Zong, Jingbo Chen, Yi Wang, Dandan Li, Ling Li, Jingjing Wang, Hailin Guo, Jianxiu Liu
Turf color is the most important characteristics of visual quality for a turfgrass species with high ornamental value and wide application prospects. Centipedegrass is a well-adapted warm-season turfgrass species in tropical, subtropical and temperate regions, possessing many outstanding properties including uniform green color. However, quite a few centipedegrass accessions or cultivars produce stolons and spike tissues with red-purple color, thereby decreasing their aesthetic value. A research focus in centipedegrass is to develop high-quality cultivars with uniform green color...
September 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29988164/concordant-divergence-of-mitogenomes-and-a-mitonuclear-gene-cluster-in-bird-lineages-inhabiting-different-climates
#4
Hernán E Morales, Alexandra Pavlova, Nevil Amos, Richard Major, Andrzej Kilian, Chris Greening, Paul Sunnucks
Metabolic processes in eukaryotic cells depend on interactions between mitochondrial and nuclear gene products (mitonuclear interactions). These interactions could have a direct role in population divergence. Here, we study mitonuclear co-evolution in a widespread bird that experienced population divergence followed by bidirectional mitochondrial introgression into different nuclear backgrounds. Using >60,000 single nucleotide polymorphisms, we quantify patterns of nuclear genetic differentiation between populations that occupy areas with different climates and harbour deeply divergent mitochondrial lineages despite ongoing nuclear gene flow...
August 2018: Nature Ecology & Evolution
https://www.readbyqxmd.com/read/29968460/cigarette-smoking-an-assessment-of-tobacco-s-global-environmental-footprint-across-its-entire-supply-chain
#5
Maria Zafeiridou, Nicholas S Hopkinson, Nikolaos Voulvoulis
While the health effects of cigarette smoking are well recognized and documented, the environmental impacts of tobacco are less appreciated and often overlooked. Here, we evaluate tobacco's global footprint across its entire supply chain, looking at resource needs, waste, and emissions of the full cradle-to-grave life cycle of cigarettes. The cultivation of 32.4 Mt of green tobacco used for the production of 6.48 Mt of dry tobacco in the six trillion cigarettes manufactured worldwide in 2014, were shown to contribute almost 84 Mt CO2 equiv emissions to climate change-approximately 0...
August 7, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29850672/the-effects-of-sr-incorporated-micro-nano-rough-titanium-surface-on-rbmsc-migration-and-osteogenic-differentiation-for-rapid-osteointegration
#6
Chuan Zhou, An-Tian Xu, Dan-Dan Wang, Guo-Fen Lin, Tie Liu, Fu-Ming He
Recruitment of endogenous bone marrow-derived mesenchymal stem cells (BMSCs) has been widely discussed as an alternative strategy for bone regeneration. Strontium (Sr) is known to direct the BMSCs' commitment to the bone lineage and encourage bone formation; however, the underlying mechanisms remain elusive. In this study, an Sr-incorporated micro/nano rough titanium surface (MNT-Sr) was fabricated by hydrothermal treatment in an attempt to facilitate BMSCs' recruitment and their osteogenic differentiation to enhance rapid osseointegration...
June 25, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29742648/comparison-of-bacterial-culture-results-obtained-from-nasopharyngeal-and-midturbinate-swabs-in-children-with-upper-respiratory-tract-infection-symptoms
#7
Judith M Martin, Anita Simonian, Gysella Muniz, Michael Green, Nader Shaikh
Paired nasopharynx and midturbinate swabs were obtained from 96 children (mean age: 2.45 years) with upper respiratory tract symptoms. Of these, 38.5% were colonized with Streptococcus pneumoniae only, 16.7% with Haemophilus influenzae only and 9.4% with both bacteria. Bacterial cultures from paired nasopharynx and midturbinate swabs demonstrated agreement for S. pneumoniae (κ = 0.75), H. influenzae (κ = 0.59) and for both pathogens (κ = 0.62). This report compares the results of bacterial cultures obtained from the nasopharynx and midturbinate specimens in children with upper respiratory tract infections...
November 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29682409/copper-induced-activation-of-trps-and-vdccs-triggers-a-calcium-signature-response-regulating-gene-expression-in-ectocarpus-siliculosus
#8
Alberto González, Claudio A Sáez, Alejandra Moenne
In certain multicellular photoautotrophs, such as plants and green macroalgae, it has been demonstrated that calcium signaling importantly mediates tolerance to copper excess. However, there is no information in brown macroalgae, which are phylogenetically distant from green algae and plants. We have previously shown that chronic copper levels (2.5 μM) activate transient receptor potential (TRP) channels in the model brown macroalga Ectocarpus siliculosus , allowing extracellular calcium entry at 13, 29, 39 and 51 min...
2018: PeerJ
https://www.readbyqxmd.com/read/29549943/green-synthesis-of-water-soluble-nontoxic-inorganic-polymer-nanocomposites-containing-silver-nanoparticles-using-white-tea-extract-and-assessment-of-their-in-vitro-antioxidant-and-cytotoxicity-activities
#9
Zeinab Haghparasti, Mahnaz Mahdavi Shahri
The use of nontoxic biological compounds in the synthesis of nanomaterials is an ecofriendly and cost-effective approach in nanotechnology. The present work was carried out to develop silver nanoparticles (Ag-NPs) by a green method using white tea (Wt) extract as reducing agent for reduction of silver nitrate as silver precursor into the lamellar space of inorganic polymer montmorillonite (Mt) as an effective protective reagent and support as well. The bioformed Wt/Ag@Mt nanocomposite (NCs) was compared with pure biosynthesized Wt/Ag-NPs under free stabilizer condition and effect of Mt stabilizer on antioxidant, cytotoxicity and structural properties were also investigated...
June 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29417735/foetal-bovine-serum-can-reduce-toxicity-of-indocyanine-green-brilliant-blue-g-and-trypan-blue-in-arpe-19-cellular-model-that-suggests-new-surgical-staining-protocols-for-internal-limiting-membrane-peeling-procedure
#10
Yu-Yi Lin, Jorn-Hon Liu, Yin Chang
BACKGROUND: To investigate and compare the cytotoxicity of indocyanine green (ICG), brilliant blue G (BBG) and trypan blue (TB) using ARPE-19 cells that have been pre-treated/post-treated with balanced salt solution (BSS) or foetal bovine serum (FBS). METHODS: The cultured human retina pigment epithelium ARPE-19 cells were pre-treated/post-treated with BSS or FBS (represent the autologous serum in clinic) in parallel with cells being soaked with various concentrations of ICG, BBG and TB...
September 2018: Clinical & Experimental Ophthalmology
https://www.readbyqxmd.com/read/29396845/resistance-of-collard-green-genotypes-to-bemisia-tabaci-biotype-b-characterization-of-antixenosis
#11
G M Domingos, E L L Baldin, V F Canassa, I F Silva, A L Lourenção
Bemisia tabaci (Genn.) biotype B (Hemiptera: Aleyrodidae) is an important pest of vegetable crops, including collard greens Brassica oleracea var. acephala (Brassicaceae). The use of resistant genotypes is an interesting option to reduce insect populations and can be used as an important tool for integrated pest management (IPM). This study evaluated 32 genotypes of collard greens against the attack of silver leaf whitefly, with the aim to characterize antixenosis. Initially, a multiple-choice trial was conducted using all genotypes, in which the adult attractiveness was assessed on two leaves per genotype at 24 and 48 h after infestation...
August 2018: Neotropical Entomology
https://www.readbyqxmd.com/read/29371795/taxonomic-revision-of-the-lichen-genera-pertusaria-varicellaria-and-variolaria-pertusariales-ascomycota-in-south-korea
#12
Jung Shin Park, Sook-Young Park, Chan-Ho Park, Sergii Y Kondratyuk, Soon-Ok Oh, Jae-Seoun Hur
The crustose lichen genus Pertusaria comprises over ca. 800 species worldwide. In total, 20 Pertusaria species were localized to the Mt. Sorak and Jeju-do in Korea. To date, information regarding the distribution of Pertusaria species in the South Korean peninsula is scarce. In this study, we collected Pertusaria species across South Korea and identified them based on morphological, chemical, and molecular characteristics. Of the 387 samples collected, we identified 24 taxa and 1 variety, of which 17 were previously recorded, and 6 taxa were newly found in South Korea ( P...
December 2017: Mycobiology
https://www.readbyqxmd.com/read/29299926/hydrophilic-multitemplate-molecularly-imprinted-biopolymers-based-on-a-green-synthesis-strategy-for-determination-of-b-family-vitamins
#13
Abbas Ostovan, Mehrorang Ghaedi, Maryam Arabi, Qian Yang, Jinhua Li, Lingxin Chen
A novel green synthesis strategy was proposed for preparation of multitemplate molecularly imprinted biopolymers (mt-MIBP) in aqueous media with less consumption of organic solvents, which were subsequently used as sorbents of ultrasound-assisted dispersive solid-phase extraction (d-SPE) for simultaneous recognition and efficient separation of B-family vitamins in juice samples, followed by high performance liquid chromatography (HPLC) determination. The obtained mt-MIBP was fully characterized by SEM, FT-IR, TEM, and BET...
January 31, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29231000/time-dependent-expression-of-mt1a-mrna-and-mt2a-mrna-in-the-contused-skeletal-muscle-of-rats
#14
H L Fan, S F Liu, J H Sun, Y Y Wang
OBJECTIVES: To investigate the time-dependent expression of metallothionein (MT) 1A mRNA and MT2A mRNA in contused skeletal muscle of rats. METHODS: A total of 54 Sprague-Dawley rats were used in this study. The rats were divided into two parts: control group ( n =6) and contusion groups (0.5, 1, 6, 12, 18, 24, 30, and 36 h after contusion, n =6). Total RNA was extracted from skeletal muscle. The expression levels of MT1A mRNA and MT2A mRNA were detected by SYBR Green I real-time PCR...
February 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29170927/climate-mitigation-low-carbon-society-and-dynamism-of-educational-institutes-in-a-low-income-country
#15
Ghaffar Ali, Shahera Anbren, Muhammad Khalid Bashir
This paper aims to investigate carbon footprints of faculty members of University of Agriculture Faisalabad (UAF) associated with income and education in pursuance of a low-carbon society. For the study, 140 UAF faculty members (professors, associate professors, assistant professors, and lecturers) were selected using stratified random sampling technique, and a representative questionnaire was used to record primary data. Moreover, the ordinary least square (OLS) method was used to explain the statistical relationship between income, education, and carbon footprints...
February 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29105619/methodological-aspects-of-modeling-household-solid-waste-generation-in-japan-evidence-from-okayama-and-otsu-cities
#16
Binxian Gu, Takeshi Fujiwara, Renfu Jia, Ruiyang Duan, Aijun Gu
This paper presents a quantitative methodology and two empirical case studies in Japan on modeling household solid waste (HSW) generation based on individual consumption expenditure (ICE) and local waste policy effects by using the coupled estimation model systems. Results indicate that ICE on food, miscellaneous commodities and services, as well as education, cultural, and recreation services are mainly associated with the changes of HSW generation and its components in Okayama and Otsu from 1980 to 2014. The effects of waste policy measures were also identified...
December 2017: Waste Management & Research
https://www.readbyqxmd.com/read/29051342/-68-ga-nota-functionalized-ubiquicidin-cytotoxicity-biodistribution-radiation-dosimetry-and-first-in-human-pet-ct-imaging-of-infections
#17
Thomas Ebenhan, Mike M Sathekge, Thabo Lengana, Michel Koole, Olivier Gheysens, Thavendran Govender, Jan R Zeevaart
Ubiquicidin is an antimicrobial peptide with great potential for nuclear imaging of infectious diseases, as its cationic-rich fragment TGRAKRRMQYNRR (UBI) has been functionalized with NOTA to allow complexation to 68 Ga (68 Ga-NOTA-UBI). We herein assess the cytotoxicity and radiation dosimetry for 68 Ga-NOTA-UBI and a first-in-human evaluation to diagnose infectious processes. Methods: Cytotoxicity was evaluated in green monkey kidney epithelial (Vero) cells and MT-4 leukocytes. Tracer susceptibility was studied in vitro using different bacterial and fungal strains...
February 2018: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28979800/maternal-smoking-during-pregnancy-is-associated-with-mitochondrial-dna-methylation
#18
David A Armstrong, Benjamin B Green, Bailey A Blair, Dylan J Guerin, Julia F Litzky, Niraj R Chavan, Kevin J Pearson, Carmen J Marsit
Maternal smoking during pregnancy (MSDP) has detrimental effects on fetal development and on the health of the offspring into adulthood. Energy homeostasis through ATP production via the mitochondria (mt) plays a key role during pregnancy. This study aimed to determine if MSDP resulted in differences in DNA methylation to the placental mitochondrial chromosome at the transcription and replication control region, the D-Loop, and if these differences were also present in an alternate neonatal tissue (foreskin) in an independent birth cohort...
August 1, 2016: Environmental Epigenetics
https://www.readbyqxmd.com/read/28963959/kinetic-analysis-of-human-metallothionein-and-cdte-quantum-dot-complexes-using-fluorescence-and-voltammetry-techniques
#19
Ewelina Guszpit, Ludmila Krejčová, Soňa Křížková, Marta Kepinska, Lukáš Richtera, Pavel Kopel, Vojtěch Adam, Halina Milnerowicz
Thanks to quantum dots' (QDs) properties, they can be used as selective and sensitive biomarkers in molecular imaging. In a previous paper, we confirmed the possibility of interaction between mercaptosuccinic acid-capped cadmium telluride QDs (MSA-CdTe) and human metallothionein (MT). The aim of this study was to expand on our previous research with an evaluation of the stability of the formed complexes between human MT and four CdTe compounds of the following sizes: 3.4nm (blue QDs), 3.8nm (green QDs), 4.5nm (yellow QDs), and 5...
December 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28962155/stable-cell-lines-of-human-sh-sy5y-uniformly-expressing-wild-type-or-mutant-type-ferm-domain-containing-7-gene
#20
Jiali Pu, Yanfang Mao, Lingjia Xu, Tingting Zheng, Baorong Zhang
It has been reported that FERM domain containing 7 (FRMD7) may cause X-linked idiopathic congenital nystagmus (ICN). A total of >40 mutations of the FRMD7 gene have been identified, however their pathogenic role remains unclear. In the present study, enhanced green fluorescent protein-tagged wild-type (WT) and mutant (MT) FRMD7 (c. C781>G) were expressed in stably expressing human neuroblastoma SH-SY5Y cells following viral transfection and antibiotic selection. Uniform expression of the FRMD7 fusion proteins was confirmed via fluorescence microscopy and western blotting...
September 2017: Experimental and Therapeutic Medicine
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