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Fei Yu, Ming Li, Zhipeng Yuan, Feng Rao, Xingxing Fang, Baoguo Jiang, Yongqiang Wen, Peixun Zhang
Background: Articular cartilage defects are difficult to treat, but drug-loaded tissue engineering scaffolds provide a possible treatment option for these types of injuries. Purpose: In this study, we designed a bioactive resveratrol-PLA-gelatin porous nano-scaffold using electrospinning, freeze drying, and uniform dispersion techniques to repair articular cartilage defects, and then investigated the possible mechanism behind the successful repair. Methods: We established an articular cartilage defect rat model with a 2 mm diameter wound in the middle of the knee joint femoral condyle non-weight-bearing area, with a depth reaching the full thickness of the subchondral bone...
2018: International Journal of Nanomedicine
Lin Ouyang, Leen Leus, Ellen De Keyser, Marie-Christine Van Labeke
We studied metabolic adaptations to cold stress in roses and identified genes in the carbohydrate pathway during acclimation and deacclimation. A field experiment with four rose cultivars belonging to different USDA plant hardiness zones was set up in Melle, Belgium (51° 0' N, 3° 48' E). The more cold-hardy cultivars ('Dagmar Hastrup' and 'John Cabot') reached their lowest LT50 value in December, indicating a rapid acclimation after the first occurrence of frost. Less cold-hardy cultivars ('Abraham Darby' and 'Chandos Beauty') reached their lowest LT50 in January/February when exposed to prolonged freezing temperatures...
December 4, 2018: Journal of Plant Physiology
F Pezo, F Romero, F Zambrano, R Sánchez
In the pork industry, artificial insemination and the storage of boar semen in liquid at 17°C are routinely applied to optimize the ejaculate and bring about rapid genetic changes that are reflected in the animal protein. Although the results are satisfactory, they are below what occurs with natural mating. It is currently possible to preserve boar semen with storage at 17°C and slow freezing, since to date there is only one study on vitrification, with negative results applicable only in the case of implementing an intracytoplasmic sperm injection...
December 8, 2018: Reproduction in Domestic Animals, Zuchthygiene
Hosein Bidgoli, Yadollah Mortazavi, Abbas Ali Khodadadi
A new synthesis strategy was adopted to convert cellulose to a biodegradable sorbent with properties of very high oil absorption and retention capacities, excellent oil-water selectivity, good mechanical strength and recycling ability. The sorbent in form of a hydrophobic/oleophilic nano-structured aerogel was prepared through functionalizing cotton cellulose with low surface energy moieties followed by dissolving and chemically cross-linking the product in an organic medium (DMSO), and freeze-drying. High absorption capacities of 40...
November 23, 2018: Journal of Hazardous Materials
Wenxiu Zhou, Xinwen Huang, Kunde Lin
The purpose of this study was to develop a novel and sensitive method for the analysis of carbazole and polyhalogenated carbazoles (PHCs) in sediment using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Briefly, 5.0 g of freeze-dried sediment samples were extracted with dichloromethane using pressurized liquid extraction (PLE). The extract was purified with Florisil solid phase extraction cartridge, filtered through 0.22 µm polytetrafluoroethylene filter using a glass syringe, followed by LC-MS/MS analysis...
December 5, 2018: Ecotoxicology and Environmental Safety
Lito E Papanicolas, Yanan Wang, Jocelyn M Choo, David L Gordon, Steve L Wesselingh, Geraint B Rogers
The efficacy of faecal microbiota transplantation (FMT) as a therapeutic intervention may depend on the viability of the microorganisms in faecal slurries (FS) prepared from donor stool. However, determining the viability of these organisms is challenging. Most microorganisms in stool are refractory to culture using standard techniques, and culture-independent PCR-based methods derive signal from both viable and non-viable cells. Propidium monoazide (PMA) treatment has been shown to be effective in preventing PCR amplification of DNA from non-viable bacteria in a range of contexts...
December 4, 2018: Journal of Microbiological Methods
Johan Unga, Daiki Omata, Nobuki Kudo, Soki Ueno, Lisa Munakata, Tadamitsu Shima, Ryo Suzuki, Kazuo Maruyama
It is known that Phosphatidyl choline-Phosphatidyl glycerol mixtures can be used for liposome formulations, making them less leaky than liposomes with only one lipid. We hypothesized that this might also be the case for bubbles, which can be used as ultrasound (US) contrast agents. Therefore, we have compared a series of mixed distearoyl phosphatidylcholine-distearoyl phosphatidylglycerol (DSPC-DPSG) bubbles and with bubbles containing either DSPC or DSPG (and distearoyl ethanolamine-polyethyleneglycol 2000, DSPE-PEG2k)...
December 10, 2018: Journal of Liposome Research
Ajoke F Idayat Apanpa-Qasim, Adebola A Adeyi
Background: Consumer products such as paints are a potentially significant source of volatile organic compounds (VOCs) and oxygenated VOCs. Paints for construction and household use have been rapidly changing from oil-based to water-based paints and are one of the commonly identified sources of oxygenated VOCs in indoor environments. Objectives: Four different anti-freezing agents were identified and analyzed in 174 waterbased paint samples, purchased from popular paint markets in two metropolitan cities in Nigeria, Lagos and Ibadan...
June 2018: Journal of health & pollution
Nataša Šterbenc, Jane M Morrell, Marjan Kosec, Detlef Rath, Sabine Klein, Primož Klinc
The cell membrane of ram spermatozoa is more sensitive to the freezing process than in other species due to its composition. As a result, the quality and viability of frozen thawed ram spermatozoa are often poor, which together with the specific structure of the ewe's cervix are the main reasons for lower fertility in ewes after intracervical insemination. In the present study we investigated the effects of semen centrifugation through a single layer of a species-specific colloid (Androcoll-O) on post-thaw quality of ram spermatozoa...
December 3, 2018: Cryobiology
Timo Domisch, Françoise Martz, Tapani Repo, Pasi Rautio
Air temperatures and precipitation are predicted to increase in the future, especially at high latitudes and particularly so during winter. In contrast to air temperatures, changes in soil temperatures are more difficult to predict, as the fate of the insulating snow cover is crucial in this respect. Soil conditions can also be affected by rain-on-snow events and warm spells during winter, resulting in freeze-thaw cycles, compacted snow, ice encasement and local flooding. These adverse conditions during winter could counteract the otherwise positive effects of climate change on forest growth and productivity...
December 4, 2018: Tree Physiology
Yuan Yuan, Qingyun Mai, Jiaxuan Ma, Mingfen Deng, Yanwen Xu, Guanglun Zhuang, Canquan Zhou
STUDY QUESTION: Do human embryos survive long-term cryopreservation (CP) (≥12 years) and implant after frozen embryo transfer (ET)? SUMMARY ANSWER: Human embryos remain usable after long-term CP. WHAT IS KNOWN ALREADY: Several cohort studies have reported the live birth rate or neonatal outcomes of human embryos after CP for up to 5 years. Only a few case reports have described successful live births from human embryos after long-term CP up to 12 years...
December 4, 2018: Human Reproduction
W J Chang, B H Chen, B S Inbaraj, J T Chien
BACKGROUND: Allyl isothiocyanate (AITC), a volatile and water-insoluble compound present in several cruciferous vegetables, has been shown to possess several biological activities such as anti-bacteria, anti-fungi and anti-cancer. In this study, water-soluble allyl isothiocyanate nanoparticles (AITC-NPs) were prepared by O/W microemulsion and complex coacervation techniques and evaluated for their anti-inflammatory and anti-cancer effects on human bladder cancer cell HT1376. RESULTS: The AITC-NPs with a particle size of 9...
December 5, 2018: Journal of the Science of Food and Agriculture
Bing Wang, Qi Fang
Three 1-phenylindolin-2-one derivatives, namely 6-chloro-1-phenylindolin-2-one (A), 4-chloro-1-phenylindolin-2-one (B) and 1-(3-chlorophenyl)indolin-2-one (C), all C14 H10 ClNO, have been synthesized and the structures of these compounds at room temperature (ART , BRT and CRT ) and low temperature (ALT , BLT and CLT ) have been determined. Crystal A at 295 K (ART ) crystallized in the monoclinic space group P21 /c and the phenyl ring of the unique molecule exhibits disorder over two positions. At low temperature, the disorder disappeared and crystal A at 90 K (ALT ) crystallized in the triclinic space group P-1 with a doubled unit-cell volume and four molecules in the asymmetric unit...
December 1, 2018: Acta Crystallographica. Section C, Structural Chemistry
Ali Mohammadi, Behzad Mansoori, Pouria Savadi, Vahid Khaze, Mahsa Minouei, Nigel A J McMillan, Somayeh Hallaj-Nezhadi, Behzad Baradaran
BACKGROUND: Considering the complex nature of gastrointestinal cancer, different methods including surgery, radiotherapy, and chemotherapy are considered for the treatment. Novel strategies including silencing of oncogenes using safe delivery systems could be considered as a novel approach in colorectal cancer treatment. The aim of this study was to investigate the silencing effect of high mobility group A2 (HMGA2) small interfering RNA (siRNA)-loaded nanoliposomes on gastrointestinal cancers...
December 2, 2018: Journal of Cellular Biochemistry
Takuro Nishimura, Kaoru Okishige, Yasuteru Yamauchi, Hideshi Aoyagi, Naruhiko Ito, Yusuke Tsuchiya, Takatoshi Shigeta, Rena Nakamura, Mitsutoshi Asano, Mitsumi Yamashita, Tomofumi Nakamura, Hidetoshi Suzuki, Tsukasa Shimura, Manabu Kurabayashi, Takehiko Keida, Tetsuo Sasano, Kenzo Hirao
Background: Complete occlusion of the pulmonary veins (PVs) with the cryoballoon (CB) is considered to be the crucial factor for a successful PV isolation (PVI). We investigated whether a complete occlusion was indispensable for a successful CB based PVI of every PV. Methods and Results: Atrial fibrillation patients (n=123, 97; paroxysmal) undergoing a de novo PVI were enrolled. A total of 477 PVs were analyzed. The occlusion grade (OG) was scored as follows: OG3 (complete occlusion), OG2 (incomplete occlusion with slight leakage), OG1 (poor occlusion with massive leakage)...
August 2018: Journal of Atrial Fibrillation
Dominique de Ziegler, Paul Pirtea, Marie Carbonnel, Marine Poulain, Ettore Cicinelli, Carlo Bulletti, Konstantinos Kostaras, George Kontopoulos, David Keefe, Jean Marc Ayoubi
Endometriosis - a disease causing pain and infertility - is encountered in nearly 50% of infertile women. While medical treatment is effective on pain and recurrence of symptoms after surgical excision, it is of no help for treating infertility for which the only options considered are surgery and ART. Surgery enhances the chances of conceiving naturally during the 12-18 ensuing months irrespective of the stage of the disease. Surgery however is of no help when ART is considered, as it does not improve outcome and can only harm the ovarian response to stimulation...
November 3, 2018: Best Practice & Research. Clinical Endocrinology & Metabolism
Bobby G Ng, Jill A Rosenfeld, Lisa Emrick, Mahim Jain, Lindsay C Burrage, Brendan Lee, William J Craigen, David R Bearden, Brett H Graham, Hudson H Freeze
FUK encodes fucokinase, the only enzyme capable of converting L-fucose to fucose-1-phosphate, which will ultimately be used for synthesizing GDP-fucose, the donor substrate for all fucosyltransferases. Although it is essential for fucose salvage, this pathway is thought to make only a minor contribution to the total amount of GDP-fucose. A second pathway, the major de novo pathway, involves conversion of GDP-mannose to GDP-fucose. Here we describe two unrelated individuals who have pathogenic variants in FUK and who presented with severe developmental delays, encephalopathy, intractable seizures, and hypotonia...
November 21, 2018: American Journal of Human Genetics
Sungjun Choi, Minjae Jeon, Byung-Kook Kim, Byoung-In Sang, Hyoungchul Kim
We probe the electrochemical performance of Li-argyrodite-based all-solid-state batteries under deep-freezing conditions (-30 °C) using electrochemical impedance spectroscopy. The performance deterioration is mainly caused by the increased interfacial resistances of electrolyte and active materials resulting from the slow kinetics of Li-ion transport in solid materials at low temperatures.
November 30, 2018: Chemical Communications: Chem Comm
Carlos A Duarte, Sheila Chávez, Yordanka Masforrol, Samy Puertas, Taimí Paneque, Anna C Ramirez, Dionne Casillas, Pedro Puente, Hilda Garay, Celia Fernández-Ortega
The synthetic peptide CIGB-210 is a promising anti-HIV drug candidate shown to inhibit HIV replication in MT4 cells at the nanomolar range by triggering the rearrangement of vimentin intermediate filaments. Sensitive and specific analytical methods are required for pharmacological studies of CIBG-210 in animals. In this study, we describe the development of a competitive ELISA for the quantitative determination of CIGB-210 using an anti-CIGB-210 hyperimmune serum. After optimization of all the steps, the assay exhibited a dynamic range from 11...
November 29, 2018: Journal of Immunoassay & Immunochemistry
Masahito Takahashi, Dai Ishikawa, Takashi Sasaki, Yu Jie Lu, Kyoko Kuwahara-Arai, Masato Kamei, Tomoyoshi Shibuya, Taro Osada, Keiichi Hiramatsu, Akihito Nagahara
AIMS: There has been growing interest in fecal microbiota transplantation (FMT) as treatment. Although, frozen donor feces preserved at -20°C has been widely used for practical advantages, freezing at -20°C can affect bacterial viability. Adequacy evaluation of fresh and frozen feces as the transplant is necessary for the methodological improvement of FMT. METHODS AND RESULTS: The viable bacterial compositions of fecal specimens under fresh and freezing conditions were compared by a microbiome analysis using propidium monoazide (PMA microbiome)...
November 29, 2018: Journal of Applied Microbiology
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