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Lucie Gavin-Plagne, Loris Commin, Pierre Bruyère, Samuel Buff, Thierry Joly
Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem Alpha, Saint-Genis-l'Argentière, France), called "CRYO3," is a chemically defined preservation medium currently used for freezing human tissue and adult stem cells. The aim of this study was to evaluate the effect of a CRYO3-based medium on ram sperm freezing regarding in vitro parameters and in vivo fertility...
October 16, 2018: Biopreservation and Biobanking
Ewa Mosiniewicz-Szablewska, Antonia R Clavijo, Ana P O R Castilho, Leonardo G Paterno, Marcelo A Pereira-da-Silva, Jarosław Więckowski, Maria A G Soler, Paulo C Morais
This study reports on investigation of the magnetic properties of layer-by-layer (LbL) assembled nanofilms comprising polyvinyl alcohol (PVA) and citrate-coated magnetite (cit-MAG) nanoparticles deposited onto silicon (SF sample) and glass (GF sample) substrates. DC magnetization measurements were performed over the temperature range of 4 K to 300 K, in the applied magnetic field range of ±60 kOe. The magnetic data of the as-synthesized cit-MAG nanoparticles (F sample) are also collected for comparison. The three as-fabricated samples reveal perfect superparamagnetic (SPM) behavior only around room temperature; at temperatures lower than 200 K the SPM scaling is not observed and all samples behave as interacting superparamagnetic (ISPM) materials...
October 16, 2018: Physical Chemistry Chemical Physics: PCCP
Hannah C M Niermann, Anna Tyborowska, Antonius H N Cillessen, Marjolein M van Donkelaar, Femke Lammertink, Megan R Gunnar, Barbara Franke, Bernd Figner, Karin Roelofs
Given the long-lasting detrimental effects of internalizing symptoms, there is great need for detecting early risk markers. One promising marker is freezing behavior. Whereas initial freezing reactions are essential for coping with threat, prolonged freezing has been associated with internalizing psychopathology. However, it remains unknown whether early life alterations in freezing reactions predict changes in internalizing symptoms during adolescent development. In a longitudinal study (N = 116), we tested prospectively whether observed freezing in infancy predicted the development of internalizing symptoms from childhood through late adolescence (until age 17)...
October 15, 2018: Developmental Science
Chandravati Yadav, Pradip K Maji
This study investigates the synergistic potential of natural bio-extracts for preparing "all-natural" composite bio-sponges of sodium alginate (SA) with the reinforcement of a natural bio-nanomaterial i.e., cellulose nanofibres (CNFs). Aqueous suspensions of SA and CNFs in various combinations of bio-extracts (Rice water (Rw) and Giloy extract (Ge)) were freeze-dried to obtain the composite bio-sponges. Composites prepared using Rw resulted in structurally more stable samples with porosity above 75% that showed a compact honeycomb-like microstructure with interlocked CNFs network structures...
January 1, 2019: Carbohydrate Polymers
Alex Jesús Hernández-Granados, Moisés Armides Franco-Molina, Erika Evangelina Coronado-Cerda, Pablo Zapata-Benavides, Edgar Mendoza Gamboa, Yareellys Ramos-Zayas, Silvia Elena Santana-Krímskaya, Cristina Rodríguez-Padilla
Whole tumor cell lysates consist of a mixture of tumor antigens and danger associated molecular patterns (DAMPs) that can be used for dendritic cell maturation and consequently for the activation of a polyclonal T cell-specific tumor response. We evaluated the in vitro efficacy of three different preparations of canine transmissible venereal tumor (TVT) cell lysates: hypochlorous acid-whole tumor cell lysates (HOCl-L), heat shock-whole tumor cell lysates (HS-L), and freeze-thaw cycles-whole tumor cell lysates (FT-L) for the maturation of canine-derived dendritic cells...
October 5, 2018: Research in Veterinary Science
Jacques Donnez, Marie-Madeleine Dolmans
At the dawn of humanity, it was rare to live beyond the age of 35 years, so the ovary was intended to function for a woman's entire life. Nowadays, it is not unusual for women to live into their 80s. This means that many of them spend 30-40% of their lives in the menopause at increased risk of various conditions associated with an absence of oestrogens (cardiovascular disease, bone mineral density loss). Reimplantation of frozen-thawed ovarian tissue is able to restore long-term ovarian endocrine function that can persist for more than 7 years (12 years if the procedure is repeated)...
September 2018: Reproductive Biomedicine Online
Jasmine Tomar, Carin Biel, Cornelis A M de Haan, Peter J M Rottier, Nikolai Petrovsky, Henderik W Frijlink, Anke Huckriede, Wouter L J Hinrichs, Ben Peeters
Bird to human transmission of high pathogenicity avian influenza virus (HPAIV) poses a significant risk of triggering a flu pandemic in the human population. Therefore, vaccination of susceptible poultry during an HPAIV outbreak might be the best remedy to prevent such transmissions. To this end, suitable formulations and an effective mass vaccination method that can be translated to field settings needs to be developed. Our previous study in chickens has shown that inhalation of a non-adjuvanted dry powder influenza vaccine formulation during normal breathing results in partial protection against lethal influenza challenge...
October 9, 2018: European Journal of Pharmaceutics and Biopharmaceutics
Bo-Yi Jhou, Wei-Chen Fang, Yen-Lien Chen, Chin-Chu Chen
Cordyceps militaris ( C. militaris ) is a parasitic fungus that grows on the larvae of Lepidoptera . It is a well-known fungus with immunomodulatory activity. The study was conducted to clarify the edible safety of C. militaris mycelium for long term use. Eighty Sprague-Dawley (SD) rats were divided into four groups (10 males and 10 females in each group). Rats were orally administrated with reverse osmosis water or 2000, 3000 and 4000 mg per kg BW per day freeze dried C. militaris mycelium powder for 90 consecutive days...
September 1, 2018: Toxicology Research
Gwanhee Ehm, Woong-Woo Lee, Yu Jin Jung, Han-Joon Kim, Beomseok Jeon
Some of patients with Parkinson's disease (PD) have abnormal tandem gait (TG) performance without any symptoms and signs of cerebellar dysfunction. Clinical difference between patients with good and poor TG performance has not yet been studied. We report the relationship between tandem gait performance and clinical characteristics including 37 patients with PD who had no evidence of cerebellar dysfunction. Using tandem gait test, the patients were divided into two groups (good-TG and poor-TG). We evaluated the two groups with Montreal Cognitive Assessment (MoCA), Mini Mental Status Examination (MMSE), Frontal Assessment Batter (FAB), Beck Depression Index (BDI-II), REM Sleep Behavior Disorder Single-Question Screen (RBD1Q), MDS-UPDRS items related to axial disability and freezing...
October 8, 2018: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
Wenjiang Dong, Rongsuo Hu, Yuzhou Long, Hehe Li, Yanjun Zhang, Kexue Zhu, Zhong Chu
In this study, room-temperature drying, solar drying, heat pump drying (HPD), hot-air drying, and freeze drying were applied to investigate the volatile profiles and taste properties of roasted coffee beans by using electronic nose, electronic tongue, and headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS). Results indicated that the drying process markedly affected pH, total titratable acidity, total solids, and total soluble solids. Significant differences existed among all samples based on drying method; and the HPD method was superior for preserving ketones, phenols, and esters...
January 30, 2019: Food Chemistry
Eva González-Menéndez, Lucía Fernández, Diana Gutiérrez, Ana Rodríguez, Beatriz Martínez, Pilar García
Bacteriophages have been proven as effective antimicrobial agents in the treatment of infectious diseases and in other biocontrol applications including food preservation and disinfection. The extensive use of bacteriophages requires improved methodologies for medium- and long-term storage as well as for easy shipping. To this aim, we have determined the stability of four Staphylococcus phages (phiIPLA88, phiIPLA35, phiIPLA-RODI and phiIPLA-C1C) with antimicrobial potential at different temperatures (20°C/25°C, 4°C, -20°C, -80°C, -196°C) and during lyophilization (freeze drying) using several stabilizing additives (disaccharides, glycerol, sorbitol and skim milk)...
2018: PloS One
Shuang Kou, Lin Chen, Wei Tu, Federico Scossa, Yamei Wang, Jun Liu, Alisdair R Fernie, Botao Song, Conghua Xie
Low temperature severely influences potato production since cultivated potato (Solanum tuberosum) is frost sensitive. However, the mechanism underlying freezing tolerance of potato is largely unknown. In the present research, we studied the transcriptome and metabolome of the freezing-tolerant wild species Solanum acaule (Aca) and freezing-sensitive cultivated S. tuberosum (Tub) to identify the main pathways and important factors related to freezing tolerance. Gene ontology (GO) and KEGG pathway annotation indicated polyamine and amino acid metabolic pathways to be specifically up-regulated in Aca under cold treatment...
October 11, 2018: Plant Journal: for Cell and Molecular Biology
Frank R Kloss, Vincent Offermanns, Anita Kloss-Brandstätter
OBJECTIVES: The aim of this study was to compare three-dimensional alterations following the use of autogenous versus allogeneic onlay grafts for augmentation at single tooth defects. MATERIALS AND METHODS: Alveolar bone width at specific implant sites were assessed using sagittal and cross-sectional CBCT images prior grafting and at three subsequent time points. 21 patients received autogenous bone blocks harvested from the retromolar region and another 21 patients received freeze-dried cancellous allogeneic bone blocks...
October 10, 2018: Clinical Oral Implants Research
Alexander Pott, Kerstin Petscher, Michael Baumhardt, Tilman Stephan, Manuel Rattka, Rima Paliskyte, Carlo Bothner, Mirjam Keßler, Wolfgang Rottbauer, Tillman Dahme
Observation of the time-to-pulmonary vein isolation (TTI) by a spiral mapping catheter has emerged as a valuable procedural parameter in cryoballoon pulmonary vein isolation (PVI). The 1st generation spiral mapping catheter (Achieve, SMC1) has been available as an 8-polar catheter with a distal loop diameter of 15 or 20 mm. The novel spiral mapping catheter (Achieve Advance, SMC2) was designed as a true guidewire and is available, in addition to the sizes of the SMC1, as a 10-polar mapping catheter with a distal loop diameter of 25 mm...
October 9, 2018: Heart and Vessels
Elodie Drapeau, Mohammed Riad, Yuji Kajiwara, Joseph D Buxbaum
Phelan-McDermid syndrome (PMS) is a rare genetic disorder in which one copy of the SHANK3 gene is missing or mutated, leading to a global developmental delay, intellectual disability (ID), and autism. Multiple intragenic promoters and alternatively spliced exons are responsible for the formation of numerous isoforms. Many genetically-modified mouse models of PMS have been generated but most disrupt only some of the isoforms. In contrast, the vast majority of known SHANK3 mutations found in patients involve deletions that disrupt all isoforms...
May 2018: ENeuro
Dirk Prochnau, Konstantin von Knorre, Hans-Reiner Figulla, P Christian Schulze, Ralf Surber
Background: We sought to evaluate a temperature-guided approach of cryoballoon (CB) ablation without visualization of real-time recordings. Methods and results: We analysed 166 patients (34.9% female, 60 ± 11 years) with paroxysmal or short-term persistent atrial fibrillation (AF). Comorbidities included diabetes mellitus (n = 28), coronary artery disease (n = 24), hypertension (n = 122), previous stroke or TIA > 3 months (n = 12)...
December 2018: IJC Heart & Vasculature
Andrey Nikolayevich Khudyakov, Larisa Georgievna Kuleshova, Oksana Olegovna Zaitseva, Marta Igorevna Sergushkina, Konstantin Aleksandrovich Vetoshkin, Tatyana Vitalyevna Polezhaeva
The ability of various pectin polysaccharides to modify the morphological structure of ice during the phase transitions from water to ice was studied. Pectins were isolated from Sosnowsky's hogweed Heracleum sosnowskyi Manden (heracleuman-N6HS), tansy Tanacetum vulgare L. (tanacetan-N7TVF), and Rauwolfia serpentina Benth callus (rauwolfian-N8RS). Pectins were isolated by multistep extraction. The effect of pectins was assessed using osmometry, thermographic analysis, and cryomicroscopy. A concentrate of leukocytes was used as the sample for the subsequent freezing step...
October 9, 2018: Biopreservation and Biobanking
Fabiano V Costa, Luiz V Rosa, Vanessa A Quadros, Adair R S Santos, Allan V Kalueff, Denis B Rosemberg
Pain, a severely debilitating symptom of many human disorders, is a growing, unmet biomedical problem. Although the use of zebrafish (Danio rerio) to investigate both behavioral and physiological nociception-related responses is expanding rapidly, the characterization of behavioral phenotypes that reflect injury location is still poorly explored, making the results of such studies difficult to interpret. Here, we characterize putative nociception-related behavioral phenotypes in adult zebrafish following an intraperitoneal (i...
October 5, 2018: Behavioural Brain Research
Peter A Kavsak, Andrew Worster, Stephen A Hill, Allan S Jaffe
BACKGROUND: International recommendations on high-sensitivity cardiac troponin (hs-cTn) testing recommend that laboratories select only one sample type for testing. We evaluated the Siemens ADVIA Centaur hs-cTnI assay in serum and thereby provide information on imprecision, long-term storage stability, freeze-thaw stability, method comparison to other hs-cTnI assays, and clinical performance. METHODS: Patients with chest pain onset <6 h who already had Roche hs-cTnT, Beckman hs-cTnI and Abbott hs-cTnI results recorded and had non-thawed and frozen serum aliquots formed the study population (n = 134 patients with 305 serum aliquots obtained at either 0, 3 or 6 h stored below -70 °C since 2003) for measurement with the Siemens hs-cTnI assay in 2018...
October 6, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Hongjie Xie, Yuli Sun, Biao Cheng, Siming Xue, Dan Cheng, Linli Liu, Lingchao Meng, Sheng Qiang
Cold stress is a major abiotic factor plants face during their life cycle. Although plants often exhibit phenotypic variation in cold tolerance, the underlying mechanism remains poorly understood. In the present study, the 50% lethal temperature (LT50) values of 37 Arabidopsis thaliana accessions at latitudes from 15° to 58° ranged from -13.2 °C to -4.9 °C and were closely correlated with the cold climates of the collection sites. According to a methylation analysis of all C-repeat (CRT)-binding factor (CBF) pathway genes, the coding and promoter regions of AtICE1, a regulator of CBF genes, exhibited the greatest variability in methylation levels among the accessions and included 5 to 122 methylated cytosine residues...
October 6, 2018: Plant & Cell Physiology
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