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Pasquale Varriale, Antoine Collomb-Clerc, Angele Van Hamme, Anaik Perrochon, Gilles Kemoun, Giuseppe Sorrentino, Nathalie George, Brian Lau, Carine Karachi, Marie-Laure Welter
OBJECTIVE: Freezing of gait (FOG) represents a major burden for Parkinson's disease (PD) patients. High-frequency (130-Hz) subthalamic deep-brain-stimulation (STN-DBS) has been reported to aggravate FOG whereas lowering the frequency to 60-80 Hz improves FOG. To further understand the effects of STN-DBS on FOG, we assessed the effects of 80-Hz and 130-Hz STN-DBS on gait initiation performance, in relation to motor and executive function processing. METHODS: Gait initiation was recorded in 19 PD patients and 20 controls, combined or not with a cognitive interference task with a modified Stroop paradigm...
August 2, 2018: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
Konstantin A Okotrub, Valentina I Mokrousova, Sergei Ya Amstislavsky, Nikolay V Surovtsev
Embryo and oocyte cryopreservation is a widely used technology for cryopreservation of genetic resources. One limitation of cryopreservation is the low tolerance to freezing observed for oocytes and embryos rich in lipid droplets. We apply Raman spectroscopy to investigate freezing of lipid droplets inside cumulus-oocyte complexes, mature oocytes, and early embryos of a domestic cat. Raman spectroscopy allows one to characterize the degree of lipid unsaturation, the lipid phase transition from the liquid-like disordered to solid-like ordered state, and the triglyceride polymorphic state...
August 7, 2018: Biophysical Journal
Phillip Dixon, Kristin Powell, Anuj Chauhan
Cystinosis is a genetic disorder that leads to the formation of cystine crystals in many organs in the body including cornea. Ocular manifestation of this disease is treated by eye drops of cysteamine which can easily oxidize into its disulfide cystamine. The rapid oxidation limits the shelf life as well the duration during which the drug can be used after opening the eye drop bottle. We evaluate two approaches of preventing the oxidation of cysteamine with the goal of increasing the time of use after opening the bottle to one month...
August 9, 2018: International Journal of Pharmaceutics
Carsten Balczun, Patrick L Scheid
Free-living amoebae (FLA) are protozoa ubiquitously found in nature. As some species or strains of these FLA are pathogenic for humans and animals, they represent objects of medical and parasitological research worldwide. Storage of valuable FLA strains in laboratories is often time- and energy-consuming and expensive. The shipment of such strains as frozen stocks is cumbersome and challenging in terms of cooling requirements as well as of transport regulations. To overcome these difficulties and challenges in maintenance and transport, we present a new method to generate lyophilised samples of non-cyst-forming FLA (Ripella (Vannella) spp...
August 9, 2018: Parasitology Research
Alyssa Hamlin, Jessica L Bauer, Alex J Polotsky, Shona C Murray
Patients undergoing assisted reproduction are advised to abstain from intercourse to prevent the possibility of multiple pregnancy. If patients do not follow this advice, multiple dizygotic pregnancy or even a heterotopic pregnancy can result. We report the case of a 28-year-old nulliparous female with unexplained infertility who underwent freeze-all vaginal oocyte retrieval. Twenty-one days later she presented with vaginal bleeding (similar to menstruation) and right lower-quadrant pain. The results of ultrasound scanning and a laboratory work-up were consistent with an ectopic pregnancy...
July 2018: Case Reports in Women's Health
Lian He, Xiaoteng Yan, Jian Liang, Shijie Li, Hongrui He, Qingping Xiong, Xiaoping Lai, Shaozhen Hou, Song Huang
The purpose of this study was to screen the optimum extraction of polysaccharides (DOP) from Dendrobium officinale stem. Firstly, different methods, including hot water extraction (HWE), cold-pressing (CP), freeze-thawing cold-pressing (FTCP), ultrasonic-assisted hot water extraction (UHWE), microwave-assisted hot water extraction (MHWE) and enzyme-assisted hot water extraction (EHWE), were employed to extract DOP under their respective best parameters. Then, the extraction yield, structure and antioxidant activity of the polysaccharides from different extraction methods were compared under the same condition...
October 15, 2018: Carbohydrate Polymers
Carla Kühn, Franziska Kupke, Susanne Baldermann, Rebecca Klopsch, Evelyn Lamy, Silke Hornemann, Andreas F H Pfeiffer, Monika Schreiner, Franziska S Hanschen, Sascha Rohn
SCOPE: Different metabolic and excretion pathways of the benzyl glucosinolate breakdown products benzyl isothiocyanate and benzyl cyanide were investigated to obtain information about their multiple fate after ingestion. Detailed focus was on the so far underestimated transformation/excretion pathways - protein conjugation and exhalation. METHODS AND RESULTS: Metabolites, protein conjugates, and non-conjugated isothiocyanates were determined in plasma, urine, and breath of seven volunteers after consuming freeze-dried nasturtium or bread enriched with nasturtium...
August 9, 2018: Molecular Nutrition & Food Research
Cécile Angebault, Amine Ghozlane, Stevenn Volant, Françoise Botterel, Christophe d'Enfert, Marie-Elisabeth Bougnoux
BACKGROUND: The human intestinal microbiota contains a vast community of microorganisms increasingly studied using high-throughput DNA sequencing. Standardized protocols for storage and DNA extraction from fecal samples have been established mostly for bacterial microbiota analysis. Here, we investigated the impact of storage and DNA extraction on bacterial and fungal community structures detected concomitantly. METHODS: Fecal samples from healthy adults were stored at -80°C as such or diluted in RNAlater® and subjected to 2 extraction protocols with mechanical lysis: the Powersoil® MoBio kit or the International Human Microbiota Standard (IHMS) Protocol Q...
2018: PloS One
Cheng-Hao Zhang, Yan-Lin Jiang, Liang-Ju Ning, Qi Li, Wei-Li Fu, Ya-Jing Zhang, Yan-Jing Zhang, Chun-Chao Xia, Jian Li, Ting-Wu Qin
BACKGROUND: Achilles tendon (AT) defects frequently occur in trauma and chronic injuries. Currently, no method can satisfactorily reconstruct the AT with completely restored function. PURPOSE: To evaluate the postoperative outcomes of AT defect reconstruction with decellularized bovine tendon sheets (DBTSs) in a rabbit model. STUDY DESIGN: Controlled laboratory study. METHODS: DBTSs were prepared from bovine tendons after compression, decellularization, antigen extraction, freeze drying, and sterilization...
August 3, 2018: American Journal of Sports Medicine
Ramy Boles, Justin P Gawaziuk, Nora Cristall, Sarvesh Logsetty
OBJECTIVE: To describe frostbite (freezing cold injured tissue) in children and intrinsic (psychological and behavioral) and extrinsic (meteorological and safety hazard) factors related to the injury. METHODS: Retrospective chart review of children <18 years old referred to a regional pediatric hospital for frostbite, to determine the demographics, environment, and risk factors related to injuries. RESULTS: 47 patients met the inclusion criteria...
July 30, 2018: Burns: Journal of the International Society for Burn Injuries
Randi H Goldman, Catherine Racowsky, Leslie V Farland, Janis H Fox, Santiago Munné, Lia Ribustello, Elizabeth S Ginsburg
PURPOSE: To determine the expected out-of-pocket costs of IVF with preimplantation genetic testing for aneuploidy (PGT-A) to attain a 50%, 75%, or 90% likelihood of a euploid blastocyst based on individual age and AMH, and develop a personalized counseling tool. METHODS: A cost analysis was performed and a counseling tool was developed using retrospective data from IVF cycles intended for PGT or blastocyst freeze-all between January 1, 2014 and August 31, 2017 (n = 330) and aggregate statistics on euploidy rates of > 149,000 embryos from CooperGenomics...
July 31, 2018: Journal of Assisted Reproduction and Genetics
Gabriela Castro, Inmaculada Carpinteiro, Isaac Rodríguez, Rafael Cela
The current study presents a single step sample preparation procedure for the simultaneous determination of five antihypertensive (propranolol, losartan, irbesartan, telmisartan, and valsartan), three antiarrhythmic drugs (flecainide, dronedarone, and amiodarone), and one of their metabolites (N-desethylamiodarone) in sludge from municipal sewage treatment plants (STPs). Matrix solid-phase dispersion (MSPD) and ultra-performance liquid chromatography (UPLC) with tandem mass spectrometry (MS/MS) detection were selected as sample preparation and determination techniques, respectively...
July 30, 2018: Analytical and Bioanalytical Chemistry
Henggang Ren, Yue Yang, Changgui Gu, Tongfeng Weng, Huijie Yang
Scale invariance in stride series, namely, the series shows similar patterns across multiple time scales, is used widely as a non-invasive identifier of health assessment. Detailed calculations in literature with standard tools such as De-trended Fluctuation Analysis (DFA) and Wavelet Transform Modulus Maxima (WTMM) seem to lead a conclusion that patients suffering from neurodegenerative diseases have weakened fractal gait rhythm compared with healthy persons. These variance based methods are dynamical mechanism dependent, namely, for some dynamical process the scale invariance can not be detected qualitatively, while for some others the scale invariance can be detected correctly, but the estimated value of scaling exponent is not correct...
July 30, 2018: IEEE Transactions on Neural Systems and Rehabilitation Engineering
Xiaoqin Cheng, Dong Ma, Guoqiang Fei, Zhengyao Ma, Feng Xiao, Qiujian Yu, Xiaoli Pan, Feng Zhou, Lei Zhao, Chunjiu Zhong
Thiamine and its phosphate esters play vital physiological roles and thiamine deficiency causes deleterious effects on human body. It is important to quantify accurately the thiamine metabolites in body fluids. However, due to the lack of appropriate internal standards, poor inter-laboratory standardization and time-consuming pretreatment procedure, the existing methods are limited in clinical applications. Hence, we developed a single-step HPLC-MS/MS method for accurate and precise measurement of thiamine and its phosphate esters in human whole blood...
July 23, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Piotr Lewczuk, Natalia Ermann, Ulf Andreasson, Christian Schultheis, Jana Podhorna, Philipp Spitzer, Juan Manuel Maler, Johannes Kornhuber, Kaj Blennow, Henrik Zetterberg
BACKGROUND: A growing body of evidence suggests that the plasma concentration of the neurofilament light chain (NfL) might be considered a plasma biomarker for the screening of neurodegeneration in Alzheimer's disease (AD). METHODS: With a single molecule array method (Simoa, Quanterix), plasma NfL concentrations were measured in 99 subjects with AD at the stage of mild cognitive impairment (MCI-AD; n = 25) or at the stage of early dementia (ADD; n = 33), and in nondemented controls (n = 41); in all patients, the clinical diagnoses were in accordance with the results of the four core cerebrospinal fluid (CSF) biomarkers (amyloid β (Aβ)1-42, Aβ42/40, Tau, and pTau181), interpreted according to the Erlangen Score algorithm...
July 28, 2018: Alzheimer's Research & Therapy
Pamela Leonardi, Federico Puliga, Mirco Iotti, Federica Piattoni, Alessandra Zambonelli
Ganoderma lucidum (Curtis) P. Karst., commonly used in traditional Chinese medicine, is characterized by strong genetic and phenotypic variability that reflects its active components. To preserve such a source of pharmacologically active metabolites, specimens must be collected from different geographic regions and their genetic integrity ensured during storage. To this aim, we tested the effect of ultra-low freezing (ULF) at -120°C on the vitality, mycelial growth rate, and fruiting ability of 3 Italian strains of G...
2018: International Journal of Medicinal Mushrooms
Julian H Gitter, Raimund Geidobler, Ingo Presser, Gerhard Winter
The aim of this study was to investigate if mechanistically different controlled ice nucleation techniques in freeze-drying are comparable to each other with respect to drying process performance and product quality attributes. Therefore, we studied three different model formulations including amorphous (sucrose, trehalose) and semi-crystalline (mannitol:sucrose 4:1) solids containing a monoclonal antibody IgG1 (5 g/L) processed either by application of ice fog or depressurization technique setting an ice nucleation temperature of -5°C...
July 25, 2018: Journal of Pharmaceutical Sciences
M J Blikra, T Løvdal, M R Vaka, I S Roiha, B T Lunestad, C Lindseth, D Skipnes
BACKGROUND: There is a fast increase in the number of seaweed farms in the Western World, and it is crucial for these companies and their customers to have standardized methods for quality assessment and optimization. The aim of this study was to adapt known methods for food quality determination for analysis of seaweed quality, including color, texture and microbiology, and discuss optimal heat treatments for the popular macroalgae Saccharina latissima and Alaria esculenta. RESULTS: The development of a desirable, green color during heating was highly specific to species, freezing history and part of the thallus...
July 27, 2018: Journal of the Science of Food and Agriculture
Zhao Namula, Maki Hirata, Manita Wittayarat, Fuminori Tanihara, Nhien Nguyen Thi, Takayuki Hirano, Masahiro Nii, Takeshige Otoi
Chlorogenic acid (CGA) and caffeic acid (CA) are potent antioxidants that are mostly found in coffee beans. This study aimed to investigate the effects of CGA and CA supplementation during semen freezing on the quality of frozen-thawed boar spermatozoa. The antioxidants CGA and CA were added to a semen extender to achieve final concentrations of 50, 100, 200 and 400 μM. Supplementation of 100 μM CGA and CA yielded a significantly higher percentage of sperm viability (increased by 8 - 10%) and plasma membrane integrity (increased by 4 - 6%) than the control groups without the antioxidants at 0 h and 3 h after thawing (P < 0...
July 27, 2018: Reproduction in Domestic Animals, Zuchthygiene
Xiaoxia Li, Weiguang Yang, Shu Liu, Xiu-Qing Li, Junting Jia, Pincang Zhao, Liqin Cheng, Dongmei Qi, Shuangyan Chen, Gongshe Liu
Adverse environmental stresses affect plant growth and crop yields. Sheepgrass (Leymus chinensis (Trin.) Tzvel), an important forage grass that is widely distributed in the east of Eurasia steppe, has high tolerance to extreme low temperature. Many genes that respond to cold stress were identified in sheepgrass by RNA-sequencing, but more detailed studies are needed to dissect the function of those genes. Here, we found that LcFIN2, a sheepgrass freezing-induced protein 2, encoded a chloroplast-targeted protein...
July 26, 2018: Physiologia Plantarum
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