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https://www.readbyqxmd.com/read/28437167/the-fgvps39-fgvam7-fgsso1-complex-mediates-vesicle-trafficking-and-is-important-for-the-development-and-virulence-of-fusarium-graminearum
#1
Bing Li, Luping Liu, Ying Li, Xin Dong, Haifeng Zhang, Huaigu Chen, Xiaobo Zheng, Zhengguang Zhang
Vesicle trafficking is an important event in eukaryotic organisms. Many proteins and lipids transported between different organelles or compartments are essential for survival. These processes are mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins, Rab-GTPases, and multisubunit tethering complexes such as class C core vacuole or endosome tethering and homotypic fusion or vacuole protein sorting (HOPS). Our previous study has demonstrated that FgVam7, which encodes a SNARE protein involving in vesicle trafficking, plays crucial roles in growth, asexual or sexual development, deoxynivalenol production, and pathogenicity in Fusarium graminearum...
April 24, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28436830/antimicrobial-activity-of-free-and-liposome-encapsulated-thymol-and-carvacrol-against-salmonella-and-staphylococcus-aureus-adhered-to-stainless-steel
#2
Juliana Both Engel, Caroline Heckler, Eduardo Cesar Tondo, Daniel Joner Daroit, Patrícia da Silva Malheiros
Antimicrobial activity of thymol, carvacrol and thymol/carvacrol liposomes (TCL) was evaluated against two bacterial pools, each one consisting of four strains of Staphylococcus aureus or Salmonella enterica. TCL were prepared using thin-film hydration, showing 270.20nm average diameter (polydispersity index of 0.33) and zeta potential of +39.99mV. Minimum inhibitory concentration (MIC) of thymol, carvacrol and TCL against S. aureus pool was 0.662mg/ml, while MIC for Salmonella pool was 0.331mg/ml for thymol and carvacrol, and for TCL was 0...
April 13, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28433055/pyrenyl-carbon-nanostructures-for-ultrasensitive-measurements-of-formaldehyde-in-urine
#3
Gayan Premaratne, Sabrina Farias, Sadagopan Krishnan
Measurement of ultra-low (e.g., parts-per-billion) levels of small-molecule markers in body fluids (e.g., serum, urine, saliva) involves a considerable challenge in view of designing assay strategies with sensitivity and selectivity. Herein we report for the first time an amperometric nano-bioelectrode design that uniquely combines 1-pyrenebutyric acid units pi-pi stacked with carboxylated multiwalled carbon nanotubes on the surface of gold screen printed electrodes for covalent attachment of NAD(+) dependent formaldehyde dehydrogenase (FDH)...
June 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28431269/oxytocin-in-the-postnatal-period-associations-with-attachment-and-maternal-caregiving
#4
Jane Kohlhoff, Valsamma Eapen, Mark Dadds, Feroza Khan, Derrick Silove, Bryanne Barnett
BACKGROUND: This study investigated the associations of maternal oxytocin, self-reported attachment insecurity and depressive symptoms with maternal caregiving sensitivity at 3-4months postpartum, observed during the 'free play' and 'reunion' episodes of the Still Face Procedure. METHODS: 112 mothers completed questionnaires and gave blood samples to determine oxytocin plasma levels before (time 1) and after participating in the Still Face Procedure with their infant (time 2)...
March 27, 2017: Comprehensive Psychiatry
https://www.readbyqxmd.com/read/28430147/suitability-of-strain-gage-sensors-for-integration-into-smart-sport-equipment-a-golf-club-example
#5
Anton Umek, Yuan Zhang, Sašo Tomažič, Anton Kos
Wearable devices and smart sport equipment are being increasingly used in amateur and professional sports. Smart sport equipment employs various sensors for detecting its state and actions. The correct choice of the most appropriate sensor(s) is of paramount importance for efficient and successful operation of sport equipment. When integrated into the sport equipment, ideal sensors are unobstructive, and do not change the functionality of the equipment. The article focuses on experiments for identification and selection of sensors that are suitable for the integration into a golf club with the final goal of their use in real time biofeedback applications...
April 21, 2017: Sensors
https://www.readbyqxmd.com/read/28429323/protein-targeting-to-the-plastid-of-euglena
#6
Dion G Durnford, Steven D Schwartzbach
The lateral transfer of photosynthesis between kingdoms through endosymbiosis is among the most spectacular examples of evolutionary innovation. Euglena, which acquired a chloroplast indirectly through an endosymbiosis with a green alga, represents such an example. As with other endosymbiont-derived plastids from eukaryotes, there are additional membranes that surround the organelle, of which Euglena has three. Thus, photosynthetic genes that were transferred from the endosymbiont to the host nucleus and whose proteins are required in the new plastid, are now faced with targeting and plastid import challenges...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28425591/reflective-functioning-and-personality-organization-associations-with-negative-maternal-behaviors
#7
Karin Ensink, Marie-Eve Rousseau, Marko Biberdzic, Michaël Bégin, Lina Normandin
The aim of this study was to examine whether mothers who manifest insensitive and disconnected behaviors in interaction with their infants differ in terms of maternal reflective functioning (RF), personality organization, and histories of abuse. A total of 86 mother-infant dyads, 28 of them with histories of abuse, participated in the study. RF was assessed with the Adult Attachment Interview (C. George, N. Kaplan, & M. Main, 1985), and personality organization was assessed with the self-report Inventory of Personality Organization (M...
April 20, 2017: Infant Mental Health Journal
https://www.readbyqxmd.com/read/28425129/role-of-sialyl-6-sulfo-lewis-x-in-anti-tumor-immunity-against-oral-squamous-cell-carcinoma
#8
Hisato Yoshida, Hitomi Hoshino, Yoshiaki Imamura, Hitoshi Yoshimura, Kazuo Sano, Motohiro Kobayashi
BACKGROUND: Tumor-infiltrating lymphocytes (TILs) reportedly play a pivotal role in anti-tumor immunity against oral squamous cell carcinoma (OSCC); however, mechanisms governing TIL recruitment to OSCC tissues remain to be clarified. The present study was undertaken to assess a potential association between TILs and high endothelial venule (HEV)-like vessels that express sialyl 6-sulfo Lewis X (LeX). METHODS: OSCC tissue sections (n = 41) were subjected to immunohistochemistry for sialyl 6-sulfo LeX and CD34 to allow quantitation of HEV-like vessels...
April 20, 2017: Journal of Oral Pathology & Medicine
https://www.readbyqxmd.com/read/28422978/the-escrt-regulator-did2-maintains-the-balance-between-long-distance-endosomal-transport-and-endocytic-trafficking
#9
Carl Haag, Thomas Pohlmann, Michael Feldbrügge
In highly polarised cells, like fungal hyphae, early endosomes function in both endocytosis as well as long-distance transport of various cargo including mRNA and protein complexes. However, knowledge on the crosstalk between these seemingly different trafficking processes is scarce. Here, we demonstrate that the ESCRT regulator Did2 coordinates endosomal transport in fungal hyphae of Ustilago maydis. Loss of Did2 results in defective vacuolar targeting, less processive long-distance transport and abnormal shuttling of early endosomes...
April 19, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28421709/gene-environment-interaction-influences-attachment-like-style-in-mice
#10
Glenda Lassi, Valter Tucci
Attachment styles are established soon after birth and form the basis for a healthy psychological life during adulthood. Here, we investigated whether genetic background (i.e., isogenic strains: C57Bl/6N and BALB/c) and parent-of-origin (i.e., reciprocal hybrids) epigenetic effects influence attachment-like styles in mice. We discovered that a specific genetic and epigenetic assortment exerts a role on the development of a secure or insecure attachment-like style. In particular, when biological mothers raise their pups, the attachment-like style is mainly secure, independently of the genetic background...
April 19, 2017: Genes, Brain, and Behavior
https://www.readbyqxmd.com/read/28421537/satb2-ablation-impairs-hippocampus-based-long-term-spatial-memory-and-short-term-working-memory-and-immediate-early-genes-iegs-mediated-hippocampal-synaptic-plasticity
#11
Ying Li, Qiang-Long You, Sheng-Rong Zhang, Wei-Yuan Huang, Wen-Jun Zou, Wei Jie, Shu-Ji Li, Ji-Hong Liu, Chuang-Ye Lv, Jin Cong, Yu-Ying Hu, Tian-Ming Gao, Jian-Ming Li
Special AT-rich sequence-binding protein 2 (Satb2) is a protein binding to the matrix attachment regions of DNA and important for gene regulation. Patients with SATB2 mutation usually suffer moderate to severe mental retardation. However, the mechanisms for the defects of intellectual activities in patients with SATB2 mutation are largely unclear. Here we established the heterozygous Satb2 mutant mice and Satb2 conditional knockout mice to mimic the patients with SATB2 mutation and figured out the role of Satb2 in mental activities...
April 18, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28421216/kinetics-of-crystalline-nuclei-growth-in-glassy-systems
#12
Anatolii V Mokshin, Bulat N Galimzyanov
In this work, we study crystalline nuclei growth in glassy systems, focusing primarily on the early stages of the process, during which the size of a growing nucleus is still comparable with the critical size. On the basis of molecular dynamics simulation results obtained for two crystallizing glassy systems, we evaluate the growth laws of the crystalline nuclei and the parameters of the growth kinetics at temperatures corresponding to deep supercooling; herein, a statistical treatment of the simulation results is carried out using the mean-first-passage-time method...
April 19, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28420287/associations-between-early-caregiving-and-rural-low-ses-african-american-children-s-representations-of-attachment-relationships
#13
Geoffrey L Brown, Hanna C Gustafsson, W Roger Mills-Koonce, Martha J Cox
Little research has examined the legacy of early maternal care for later attachment representations among low-income and ethnic minority school-aged children. Using data from a sample of 276 rural, low-income, African-American families, this study examined associations between maternal care in infancy and children's representations of attachment figures in middle childhood. Maternal care was coded from 10-min home-based observations at 6, 15, and 24 months of age. Representations of attachment figures were assessed using the Manchester Child Attachment Story Task at 6 years of age...
April 18, 2017: Attachment & Human Development
https://www.readbyqxmd.com/read/28418109/sagittal-crest-formation-in-great-apes-and-gibbons
#14
Katharine L Balolia, Christophe Soligo, Bernard Wood
The frequency of sagittal crest expression and patterns of sagittal crest growth and development have been documented in hominoids, including some extinct hominin taxa, and the more frequent expression of the sagittal crest in males has been traditionally linked with the need for larger-bodied individuals to have enough attachment area for the temporalis muscle. In the present study, we investigate sagittal cresting in a dentally mature sample of four hominoid taxa (Pan troglodytes schweinfurthii, Gorilla gorilla gorilla, Pongo pygmaeus pygmaeus and Hylobates lar)...
April 18, 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28414709/robotic-surgery-fast-forward-to-telemedicine
#15
George B Stefano
Since the realization that certain maladies can be corrected via surgical techniques, we have been consumed by the desire to constantly advance the tools of surgery, as well as the techniques. In recent times, computer-assisted surgical tools have emerged and are growing in their use. The Puma 560 emerged in 1985 for brain biopsies and laparoscopic surgery. Prostatic surgery was performed by the Probot in 1988. In 1992, fittings in the femur were placed using the ROBODOC. These early robotic endeavors gave rise to AESOP, ZEUS and the relatively intuitive da Vinci® Surgical System...
April 17, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28410636/stent-and-leaflet-stresses-in-29-mm-second-generation-balloon-expandable-transcatheter-aortic-valve
#16
Yue Xuan, Kapil Krishnan, Jian Ye, Danny Dvir, Julius M Guccione, Liang Ge, Elaine E Tseng
BACKGROUND: Equipoise of transcatheter aortic valve replacement with surgical aortic valve replacement in intermediate-risk patients has been demonstrated. As transcatheter aortic valve replacement usage expands, questions regarding long-term durability become paramount. Valve design impacts durability with regions of increased leaflet stress being vulnerable to early failure. However, transcatheter aortic valve (TAV) leaflet stresses are unknown. The objective of this study was to determine stent and leaflet stresses of second-generation balloon-expandable TAV...
April 12, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28409628/reversion-of-p-gp-mediated-drug-resistance-in-ovarian-carcinoma-cells-with-phpma-zosuquidar-conjugates
#17
Claudia Battistella, Harm-Anton Klok
Inhibition of P-glycoprotein (P-gp) transporter is an attractive approach for the reversion of cancer-associated multidrug resistance (MDR). PHPMA-based carriers that are able to release the anticancer drug doxorubicin in the lysosomes have shown promise to reduce P-gp mediated resistance. This is attributed to the release of the drug in close proximity to the nucleus and distant from the P-gp transporter. This work presents a strategy to maximize P-gp inhibition and enhance doxorubicin cytotoxicity in cancer cells by using a dual functional PHPMA conjugate carrying both the anticancer drug doxorubicin and the P-glycoprotein inhibitor zosuquidar (Zos)...
April 14, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28409042/nephrologists-hate-the-dialysis-catheters-a-systemic-review-of-dialysis-catheter-associated-infective-endocarditis
#18
Kalyana C Janga, Ankur Sinha, Sheldon Greenberg, Kavita Sharma
A 53-year-old Egyptian female with end stage renal disease, one month after start of hemodialysis via an internal jugular catheter, presented with fever and shortness of breath. She developed desquamating vesiculobullous lesions, widespread on her body. She was in profound septic shock and broad spectrum antibiotics were started with appropriate fluid replenishment. An echocardiogram revealed bulky leaflets of the mitral valve with a highly mobile vegetation about 2.3 cm long attached to the anterior leaflet...
2017: Case Reports in Nephrology
https://www.readbyqxmd.com/read/28401851/assessing-a-change-mechanism-in-a-randomized-home-visiting-trial-reducing-disrupted-maternal-communication-decreases-infant-disorganization
#19
Susana Tereno, Sheri Madigan, Karlen Lyons-Ruth, Andre Plamondon, Leslie Atkinson, Nicole Guedeney, Tim Greacen, Romain Dugravier, Thomas Saias, Antoine Guedeney
Although randomized interventions trials have been shown to reduce the incidence of disorganized attachment, no studies to date have identified the mechanisms of change responsible for such reductions. Maternal sensitivity has been assessed in various studies and shown to change with intervention, but in the only study to formally assess mediation, changes in maternal sensitivity did not mediate changes in infant security of attachment (Cicchetti, Rogosch, & Toth, 2006). Primary aims of the current randomized controlled intervention trial in a high-risk population were to fill gaps in the literature by assessing whether the intervention (a) reduced disorganization, (b) reduced disrupted maternal communication, and (c) whether reductions in disrupted maternal communication mediated changes in infant disorganization...
May 2017: Development and Psychopathology
https://www.readbyqxmd.com/read/28401847/enhancing-executive-functioning-among-toddlers-in-foster-care-with-an-attachment-based-intervention
#20
Teresa Lind, K Lee Raby, E B Caron, Caroline K P Roben, Mary Dozier
Young children in foster care often experience adversity, such as maltreatment and lack of stability in early caregiving relationships. As a result, these children are at risk for a range of problems, including deficits in executive functioning. The Attachment and Biobehavioral Catch-up for Toddlers (ABC-T) intervention was designed to help foster parents behave in ways that promote the development of young children's emerging self-regulatory capabilities. Participants included 173 parent-toddler dyads in three groups: foster families that were randomly assigned to receive either the ABC-T intervention (n = 63) or a control intervention (n = 58), as well as low-risk parent-toddler dyads from intact families (n = 52)...
May 2017: Development and Psychopathology
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