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João Paulo Mendes Tribst, Amanda Maria de Oliveira Dal Piva, Marcela Moreira Penteado, Alexandre Luiz Souto Borges, Marco Antonio Bottino
The aim of this study was to evaluate stress distribution in an occlusal veneer according to the restorative material, restoration thickness, and cement layer thickness. A tridimensional model of a human maxillary first molar with an occlusal veneer preparation was constructed using a modeling software of finite element analysis. The model was replicated 9 times to evaluate the factors: restoration thickness (0.6, 1.2, and 1.8 mm) and cement layer thickness (100, 200, and 300 μm). Then, each model received different restorative materials (High Translucency Zirconia - [YZHT], Lithium Disilicate - [LD], Zirconia Reinforced Lithium Silicate - [ZLS], Feldspathic - [F], and Hybrid Ceramic - [HC]), totaling forty-five groups...
November 29, 2018: Brazilian Oral Research
Théo Foutel-Rodier, Agnès Thierry, Romain Koszul, Martial Marbouty
Microbial species thrive in very diverse environments and play fundamental roles in their equilibrium and dynamics. Metagenomics consists in extracting, sequencing, and studying the DNA present in ecosystems to better understand their regulation. Ideally, the maximal amount of information would be gathered from the full sequences of the genomes, episomes, and phages present in the microbial communities. Current high-throughput DNA sequencing produces reads ranging in size from a few dozen base pairs for the most commonly used technologies to several kb for emerging single-molecule real-time sequencing techniques...
2018: Methods in Enzymology
Cláudia de Almeida Ferreira Diniz, Marcus Vinícius Faleiro de Andrade, Bruno Philip Alves da Silva, Maria Lúcia Machado Duarte, Lázaro Valentin Donadon, Ricardo Guimarães, Márcia Guimarães
A simple, low cost and easy-to-operate 3D stereophotogrammetry system was developed to measure the kinematic pattern of head stabilisation during visual tasks. The system differs from commercially available ones since it: (a) takes into account the gaze motor coordination characteristics and measures the head translations quantified at the point that best represents the translations caused by the eyes' movement during visual tasks, that is, the midpoint between the eyes; (b) offers minimum restriction to the head movement and minimum interference with it; (c) innovates when using the position coordinates produced by a free-online tracker software...
November 22, 2018: Journal of Medical Engineering & Technology
Carolina de Lima Alcantara, Wanderley de Souza, Narcisa L da Cunha E Silva
Trypanosoma cruzi epimastigotes internalize macromolecules avidly by endocytosis. Previously, we identified a tubule-vesicular network likely to correspond to the early-endosomes. However, a detailed ultrastructural characterization of these endosomes was missing. Here, we combined endocytosis assays with ultrastructural data from high-resolution electron microscopy to produce a 3D analysis of epimastigote endosomes and their interactions with endocytic organelles. We showed that endocytic cargo was found in carrier vesicles budding from the cytopharynx...
September 29, 2018: Protist
Daniel Dutra Cavalcanti, Bárbara Albuquerque Morais, Eberval Gadelha Figueiredo, Robert F Spetzler, Mark C Preul
OBJECTIVE: To describe and compare surgical exposure through microsurgical cadaveric dissection of the intercollicular region afforded by the median, paramedian and extreme-lateral supracerebellar infratentorial (SCIT) approaches. METHODS: Ten cadaveric heads were dissected using the SCIT variants approaches. Neuronavigation system was used to determine tridimensional coordinates for the intercollicular zone in each route. The area of surgical and angular exposures were evaluated and determined by software analysis for each specimen...
November 14, 2018: World Neurosurgery
Alejandro González-Torres, Evelyn Gabriela Bañuelos-Villegas, Natalia Martínez-Acuña, Eric Sulpice, Xavier Gidrol, Luis Marat Alvarez-Salas
The miR-143/145 cluster is down-regulated in cervical tumor cells suggesting a role in tumorigenesis including cytoskeleton remodeling, a key event for tumor progression. The aim of the present work was to determine the role of miR-143/145 in the modulation of the myosin regulator phospho-myosin light chain (pMLC). HeLa monolayer and tridimensional cultures were transfected with miR-143 or miR-145 mimics inhibiting cell viability, proliferation, migration and invasion, mainly through miR-145. MiR-145 transfection increased pMLC levels by targeting the MYPT1 subunit of the regulatory myosin phosphatase...
December 9, 2018: Biochemical and Biophysical Research Communications
Sara Missaglia, Daniela Tavian, Laura Moro, Corrado Angelini
BACKGROUND: Deficiency of electron transfer flavoprotein dehydrogenase (ETFDH) is associated with multiple acyl-CoA dehydrogenase deficiency (MADD). This disorder is an autosomal recessive lipid storage myopathy (LSM) that exhibits a wide range of clinical features, including myopathy, weakness and multisystem dysfunctions. Many patients with late onset of MADD improve when treated with riboflavin and are also referred to as RR-MADD (riboflavin-responsive multiple Acyl-CoA dehydrogenase disorder)...
November 13, 2018: Lipids in Health and Disease
Guglielmo Fernandez Garcia, Djamilla Guettas, Vincent Montigaud, Paolo Larini, Roberta Sessoli, Federico Totti, Olivier Cador, Guillaume Pilet, Boris Le Guennic
Molecular materials that possess a toroidal moment associated to a non-magnetic ground state are known as Single-Molecule Toroics (SMTs) and are usually planar molecules. Herein, we report a Dy4 cubane, namely [Dy4(Bppd)4(µ3-OH)4(Pa)4(H2O)4]∙0.333H2O (where BppdH=1,3-Bis(pyridin-4-yl)propane-1,3-dione and PaH=2-Picolinic acid) for which magnetometry measurements and state-of-art ab initio calculations highlight SMT behavior in a tridimensional structure (3D-SMT). The in-depth theoretical analysis on the resulting low-lying energy states, along with their variation in function of the magnetic exchange pathways, allows to shed further light on the description of Single-Molecule Toroics and identify the coupling scheme that better reproduce the observed data...
November 9, 2018: Angewandte Chemie
Marta Guerra-Rebollo, Carolina Nogueira de Moraes, Cristina Alcoholado, Carolina Soler-Botija, Lourdes Sanchez-Cid, Olaia F Vila, Oscar Meca-Cortés, Sara Ramos-Romero, Nuria Rubio, José Becerra, Jeronimo Blanco, Cristina Garrido
A preclinical model of glioblastoma (GB) bystander cell therapy using human adipose mesenchymal stromal cells (hAMSCs) is used to address the issues of cell availability, quality, and feasibility of tumor cure. We show that a fast proliferating variety of hAMSCs expressing thymidine kinase (TK) has therapeutic capacity equivalent to that of TK-expressing hAMSCs and can be used in a multiple-inoculation procedure to reduce GB tumors to a chronically inhibited state. We also show that up to 25% of unmodified hAMSCs can be tolerated in the therapeutic procedure without reducing efficacy...
December 21, 2018: Molecular Therapy Oncolytics
Pierrick Moreau, Axel Cournac, Gianna Aurora Palumbo, Martial Marbouty, Shogofa Mortaza, Agnes Thierry, Stefano Cairo, Marc Lavigne, Romain Koszul, Christine Neuveut
Whether non-integrated viral DNAs distribute randomly or target specific positions within the higher-order architecture of mammalian genomes remains largely unknown. Here we use Hi-C and viral DNA capture (CHi-C) in primary human hepatocytes infected by either hepatitis B virus (HBV) or adenovirus type 5 (Ad5) virus to show that they adopt different strategies in their respective positioning at active chromatin. HBV contacts preferentially CpG islands (CGIs) enriched in Cfp1 a factor required for its transcription...
October 15, 2018: Nature Communications
Marlene Barroso, Luis-Ernesto Arriola-Guillén, Yalil-Augusto Rodríguez-Cárdenas, Gustavo-Armando Ruíz-Mora, Maria-Eugenia Guerrero, Carlos Flores-Mir
Background: The present study was performed to compare follicle dimensions of impacted mandibular third molar (IMTM) with different impaction angulations using cone beam computed tomography (CBCT). Material and Methods: Forty-nine individuals with IMTM (24 male, 25 female) were selected. Their age range was 25-55 years. The sample was divided into three IMTM groups either vertical (n=16), mesioangular (n=18) or horizontal (n=15) position based on Winter's classification (the angle between the longitudinal axis of the second and third molars)...
August 2018: Journal of Clinical and Experimental Dentistry
David Gurpegui, Felipe Ortuño, Manuel Gurpegui
BACKGROUND: The aim of this naturalistic longitudinal study was to add evidence regarding the potential of Cloninger's temperament traits (novelty seeking, harm avoidance, reward dependence and persistence) for predicting the effect of pharmacological treatment on the rates of therapeutic response and remission. METHODS: The study was based on 200 outpatients with unipolar depressive disorders of different severity and course of illness, and extended up to 6 months of follow-up...
January 15, 2019: Journal of Affective Disorders
Ramón Fuentes, Alain Arias, María Florencia Lezcano, Diego Saravia, Gisaku Kuramochi, Pablo Navarro, Fernando José Dias
The aim of this study was to analyze the general, geometric, and kinematic characteristics of the masticatory cycle's movements in a tridimensional way, using a method developed by our study group to provide a new insight into the analysis of mandibular movements due to advancement in the potential of computational analysis. Ten individuals (20.1 ± 2.69 years), molar class I, without mandibular movement problems participated in this study. The movements of the masticatory cycles, frontal and sagittal mandibular border movements, were recorded using 3D electromagnetic articulography and processed with computational scripts developed by our research group...
2018: BioMed Research International
Michele Bellesi, Luisa de Vivo, Samuel Koebe, Giulio Tononi, Chiara Cirelli
Astrocytic glycogen represents the only form of glucose storage in the brain, and one of the outcomes of its breakdown is the production of lactate that can be used by neurons as an alternative energetic substrate. Since brain metabolism is higher in wake than in sleep, it was hypothesized that glycogen stores are depleted during wake and replenished during sleep. Furthermore, it was proposed that glycogen depletion leads to the progressive increase in adenosine levels during wake, providing a homeostatic signal that reflects the buildup of sleep pressure...
2018: Frontiers in Cellular Neuroscience
Alejandra Herrera-Morande, Victor Castro-Fernández, Felipe Merino, Cesar A Ramírez-Sarmiento, Francisco J Fernández, M Cristina Vega, Victoria Guixé
During evolution, some homologs proteins appear with different connectivity between secondary structures (different topology) but conserving the tridimensional arrangement of them (same architecture). These events can produce two types of arrangements; circular permutation or non-cyclic permutations. The first one results in the N and C terminus transferring to a different position on a protein sequence while the second refers to a more complex arrangement of the structural elements. In ribokinase superfamily, two different topologies can be identified, which are related to each other as a non-cyclic permutation occurred during the evolution...
September 12, 2018: Biochimica et biophysica acta. General subjects
Barón Zárate-Kalfópulos, Héctor R Martínez-Ríos, Francisco López-Meléndez, Carla L García-Ramos, Luis M Rosales-Olivarez, Alejandro A Reyes-Sánchez
Antecedentes: La escoliosis idiopática del adolescente se define como una deformidad tridimensional de la columna vertebral que se presenta entre los 10 y los 18 años, y que se manifiesta con una curvatura vertebral en el plano coronal mayor de 10°. Esta deformidad afecta al 2-3% de la población general, pero solo el 10% del total requerirá en algún momento tratamiento quirúrgico. El método de elección para el manejo es el uso de tornillos transpediculares y barras desrotadoras...
2018: Cirugia y Cirujanos
Raquel Garcia, Elisabete P Carreiro, João P Prates Ramalho, Anthony J Burke, João C Lima, Marco D R Gomes da Silva, Ana Maria Costa Freitas, Maria João Cabrita
This work describes the development of a new selective photocontrollable molecularly imprinted-based sorbent for the selective enrichment/pre-concentration of dimethoate from spiked olive oil samples. To achieve this goal an improved molecularly imprinted strategy relying on the embedding of a functional monomer containing an azobenzene chromophore as light-responsive element, on the crosslinked tridimensional molecular imprinted network, has been assessed. To address the mechanisms underlying template recognition and uptake/release of the analyte from the functional imprinted material, computational studies using a quantum chemical approach, have been explored...
December 4, 2018: Analytica Chimica Acta
S V Guevara Perez, G de la Rosa Castolo, L Thollon, M Behr
PURPOSE: The aim of this study was to perform an exploratory analysis of the morphological variations of mandibles at diverse states of edentulousness using tridimensional geometric morphometrics. METHODS: Twelve cadaveric mandibles were selected and divided in three groups: dentate (G1), partially edentate (G2) and totally edentate (G3). CT scans, segmentation and digital reconstruction of 3D surfaces of each specimen was made. Thirteen landmarks were defined; the measurement error was determined and 3D morphometrics exploratory analysis by principal components (PCs) and PC scores was performed...
September 6, 2018: Morphologie: Bulletin de L'Association des Anatomistes
Carlos Antônio do Nascimento Santos, Radovan Borojevic, Luiz Eurico Nasciutti, Christina M Maedatakiya
To understand the molecular mechanisms involved in gastric disorders and regeneration, we need an in vitro tridimensional (3D) culture model, which can mimic the in vivo gastric microenvironment. A 3D coculture system named gastrosphere is proposed herein, composed of primary human gastric epithelial and stromal cells. The primary cultures were obtained from endoscopic gastric biopsies, and after mechanical and enzymatic dispersion, epithelial (HGE3) and stromal (HGS12) cells were expanded. After extensive immunocytochemical characterization, cells were seeded onto 96-well round bottom plates previously covered with 1% agarose...
2018: Methods in Molecular Biology
Fábio L S Costa, Maria Elena De Lima, Suely G Figueiredo, Rafaela S Ferreira, Núbia S Prates, Tetsu Sakamoto, Carlos E Salas
Background: Lethal factors are multifunctional oligomeric proteins found in the venomous apparatus of Scorpaeniformes fish. These toxins elicit not only an array of biological responses in vitro but also cardiovascular disorders and strong hemolytic, nociceptive and edematogenic activities in vivo. This work describes the cloning and molecular identification of two toxin subunits, denominated Sp-CTx-α and Sp-CTx-β, from scorpionfish venom ( Scorpaena plumieri ). Methods: The primary structures were deduced after cDNA amplification by PCR with primers from conserved sequences described in Scorpaeniformes toxins...
2018: Journal of Venomous Animals and Toxins Including Tropical Diseases
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