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Leukemia expression

Dijiong Wu, Keding Shao, Qihao Zhou, Jie Sun, Ziqi Wang, Fei Yan, Tingting Liu, Xiangping Wu, Baodong Ye, He Huang, Yuhong Zhou
BACKGROUND/AIMS: Although the cure rate of acute promyelocytic leukemia (APL) has exceeded 90%, the relapse/refractory APL that resistant to all-trans retinoic acid (ATRA) or ATO was still serious concern. Matrine (MAT) could improve the differentiation ability of ATRA-resistant APL cells. This study aimed to explore how the APL-specific fusion protein was degraded in ATRA-resistant APL with the application of MAT and ATRA. METHODS: ATRA-sensitive (NB4) and ATRA-resistant (NB4-LR1) cell lines were used...
August 16, 2018: Cellular Physiology and Biochemistry
Uri Rozovski, Michael J Keating, Zeev Estrov
The immunoglobulin heavy chain gene (IgHV) mutation status correlates with the clinical outcome of patients with chronic lymphocytic leukemia (CLL) treated with chemoimmunotherapy. Why the survival rate of patients with unmutated IgHV is worse than that of patients with mutated IgHV is unknown. CLL cells with unmutated IgHV were thought to originate from naïve B lymphocytes, whereas CLL cells with mutated IgHV were thought to arise from B cells that have undergone somatic hypermutation (SHM). Cell surface protein expression profile and gene expression studies showing that all CLL cells, regardless of their IgHV mutation status, are of postgerminal center origin, negated this hypothesis...
August 16, 2018: Acta Haematologica
Carole Nagant, Daniele Casula, Anne Janssens, Vo Thanh Phuong Nguyen, Brigitte Cantinieaux
INTRODUCTION: The discrimination of leukemia lymphoblasts (LB) in diagnosis and follow-up of B-cell progenitor acute lymphoblastic leukemia (BCP-ALL) by multiparameter flow cytometry (MFC) may be difficult due to the presence of hematogones (HG). The aim of this study was to compare lymphoblasts of BCP-ALL and HG for the expression of the most discriminating antigens. METHODS: A total of 82 bone marrow samples (39 BCP-ALL and 43 patients with HG) were analyzed using MFC...
August 16, 2018: International Journal of Laboratory Hematology
Chiara Borga, Gilseung Park, Clay Foster, Jessica Burroughs-Garcia, Matteo Marchesin, Rikin Shah, Ameera Hasan, Syed T Ahmed, Silvia Bresolin, Lance Batchelor, Teresa Scordino, Rodney R Miles, Geertruy Te Kronnie, James L Regens, J Kimble Frazer
Precursor-B cell acute lymphoblastic leukemia (pre-B ALL) is the most common pediatric cancer, but there are no useful zebrafish pre-B ALL models. We describe the first highly- penetrant zebrafish pre-B ALL, driven by human MYC. Leukemias express B lymphoblast-specific genes and are distinct from T cell ALL (T-ALL)-which these fish also develop. Zebrafish pre-B ALL shares in vivo features and expression profiles with human pre-B ALL, and these profiles differ from zebrafish T-ALL or normal B and T cells. These animals also exhibit aberrant lymphocyte development...
August 15, 2018: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
Duco Koenis, Lejla Medzikovic, Mariska Vos, Thijs J Beldman, Pieter B van Loenen, Claudia M van Tiel, Anouk Aj Hamers, Iker Otermin Rubio, Vivian de Waard, Carlie de Vries
Gene targeting via homologous recombination can occasionally result in incomplete disruption of the targeted gene. Here, we show that a widely-used Nur77-deficient transgenic mouse model expresses a truncated protein encoding for part of the amino-terminal domain of nuclear receptor Nur77. This truncated Nur77 protein is absent in a newly developed Nur77-deficient mouse strain generated using Cre-Lox recombination. Comparison of these two mouse strains using immunohistochemistry, flow cytometry and colony-forming assays shows that homologous recombination-derived Nur77-deficient mice - but not wild-type or Cre-Lox-derived Nur77-deficient mice - suffer from liver immune cell infiltrates, loss of splenic architecture, and increased numbers of bone marrow hematopoietic stem cells and splenic colony-forming cells with age...
August 15, 2018: Journal of Biological Chemistry
Chen Tian, Yue-Yang Li, Dong-Zhi Hu
Although the application of combined chemotherapy has made a great progress in treatment of adult acute myeloid leukemia (AML), the overall survival rate is still not high, mainly because of high recurrence rate and drug resistance. The treatment regimen for relapsed/refractory AML is always strong. Due to the chemotherapy drugs lacking of specific identification of tumor cells, significant adverse reactions often come out and sometimes even endanger the life of patients. In order to improve the life quality and survival rate, the new treatment strategy is urgently needed...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Dan Zhao, Hong Yuan
OBJECTIVE: The aim of this study was to explore whether rapamycin can induce the autophagy of HL-60 cells and UCH-L3 expression. METHODS: Cell proliferation activity of HL-60 cells treated with rapamycin was measured by CCK-8 assay. After the cells were treated with rapamycin for different time, the fluorescent microscopy was used to detect the cells' modality, the morphology of autophagosomes was observed by transmission electron microscopy, the mRNA levels of autophagy-related genes LC3-II and UCH-L3 were detected by real-time PCR, and the Western blot was employed to detect the expression level of UCH-L3...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jiao Mou, Jin-Qian Dai, Ming-Li Liu, Qing-Ren Ni, Yun-Jie Zhang, Jing Wen, Yan-Ping Song
OBJECTIVE: To explore the biological function of BMAL1 in human acute myeloid leukemia by means of the HL-60 cell line in whica circadian gene BMAL1 was konocked-out by the CRISPR/Cas9 technology. METHODS: Two sgRNAs for BMAL1 were designed and the PX459 knockout vectors containing the sgRNA were constructed. The activity of 2 sgRNAs was detected by T7 endonuclease I. the BMAL1 knocked out HL-60 cells were prepared by transient transfection of the target vectors into the cells...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Qin-Min Dong, Hai-Yun Li, Fu-Zhen Lei, Ru-Yu Yang
OBJECTIVE: To investigate the effect of leukodepleted blood transfusions on peripheral blood Th1/Th2 cell balance in patients with acute lymphoblastic leukemia (ALL). METHODS: Fifty-seven ALL patients in our hospital from March 2016 to August 2017 were selected, 31 of them received routine blood transfusion were enrolled in group A, and 26 patients received depleted-blood leukotransfusion were enrolled in group B, 36 cases in normal physical examination at the same period were enrolled in control group...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jiang-Rui Guo, Wei Li, Yong Wu, Xiao-Lan Lia, Shu-Xia Zhang, Yuan-Zhong Chen
OBJECTIVE: To investigate the expression and clinical significances of HGFA, Matriptase, HAI-1 and HAI-2 in patients with acute myeloid leukemia (AML). METHODS: The bone marrow samples from 91 AML patients, 41 AML patients in complete remission, and 32 normal controls were collected. Real time fluorescence quantitative RT-PCR (qRT-PCR) was used to detect the mRNA expressions levels of HGFA, Matriptase, HAI-1, HAI-2 . The expressions of these genes were compared among AML untreated group, the complete remission group and the healthy control group...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Jia-Jia Si, Wei-Min Wang, Dan Yu, Si-Lin Gan, Fei-Fei Wu, Ling Sun, Ding-Ming Wan, Xin-Sheng Xie, Yan-Fang Liu, Chong Wang, Hui Sun
OBJECTIVE: To investigate the expression of long non coding RNA RP11-69I8.3 in acute leukemia and its clinical significance. METHODS: lncRNA RP11-69I8.3 expression was detected by RT-PCR in bone marrow samples from 17 healthy controls, 32 newly diagnosed AML patients and 32 newly diagnosed ALL patients, and 25 ALL patients of complete remission after chemotherapy. Meanwhile, the clinical data were collected and the relation of lncRNA RP11-6918.3 expression with the clinical characteristics was analyzed...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Wei-Min Dong, Yang Cao, Li-Li Xiang, Yan Lin, Yue Liu, Jian-Nong Cen, Xiao-Bao Xie, Wei-Ying Gu
OBJECTIVE: To investigate the effect of all transretinoicacid(ATRA) combined with decitabine (5-Aza-2'-deoxycytidine;DAC) on DNA methylation and gene expression of p16INK4a (p16) and retinoic acid receptor β (RARβ), and to explore their combined anti neoplastic effect on U937 cells and newly diagnose delder acute myeloid leukemia(AML) patients. METHODS: The expression levels of p16 and RARβ were determined by qRT-PCR and Western blot. Methylation-specific PCR was used to analyze their methylation status...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Yan Wang, Chun Qiao, Rui Guo, Hui Wang, Hui Yang, Hai-Rong Qiu, Yu-Jie Wu, Jian-Fu Zhang, Rong Wang, Si-Xuan Qian, Jian-Yong Li
OBJECTIVE: To analyze the clinical and laboratory features of acute myeloid leukemia (AML) with cuplike nuclei morphology. METHODS: One hundred and seventy patients diagnosed with AML (M1andM2) between December 2009 and December 2016 were included in the study. Bone marrow smears were prepared for morphologic alanalysis, the immunophenotype was analyzed by flow cytometry and the RHG-banding was for conventional cytogenetic assay (CCA) ,gene mutation was detected by sequencing...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Qi-Xia Ren, Hong-Bo Zhang, Xiang Zhang, Jian Guo
OBJECTIVE: To explore expression and significance of phosphatase and tensin homolog deleted on chromosome ten (PTEN) and survivin in acute lymphoblastic leukemia (ALL). METHODS: Peripheral blood samples were collected from 68 patients with ALL in our hospital including 48 newby diagnosed patients, 13 patients in remission, 7 patients in relapse, and 20 healthy volunteers (control). The expressions of PTEN and survivin mRNA and protein were detected by real time PCR, Western blot and respectively, and clinical pathological parameter was analyzed...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Xue-Fei Zhao, Hong-Yan Wang, Xu Zhao, Huan-Chen Cheng, Wei Li, Sheng-Wei Liu, Lin Qiu, Jun Ma
OBJECTIVE: To retrospectively analyze the immunophenotyping, fusion gene and gene mutation of 30 acute lymphoblastic leukemia (ALL) cases and to investigate the relationship between the analysis results and the clinical therapeutic effect and prognosis. METHODS: Thirty All phtients were collected from the First Hospital of Harbin, Institute of Hematology and Oncology Department of Pediatrics from August 2015 to June 2016. According to the classification of FAB standard, 27 cases were B system ALL, 3 cases were T system ALL...
August 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Chenyang Huang, Haixiang Sun, Zhilong Wang, Yang Liu, Xi Cheng, Jingyu Liu, Ruiwei Jiang, Xindong Zhang, Xin Zhen, Jidong Zhou, Linjun Chen, Lijun Ding, Guijun Yan, Yue Jiang
Recurrent implantation failure (RIF) caused by various etiological factors remains a challenge for fertility clinicians using assisted reproductive technology (ART) worldwide. Dysregulation of leukemia inhibitory factor (LIF) in the endometria of women with RIF is involved in impaired endometrial receptivity and embryo adhesion. However, the mechanism through which LIF expression is regulated in women with RIF is still poorly understood. Our previous study noted that the abnormally increased endometrial Krüppel-like factor 12 (KLF12) in RIF women led to impaired decidualization and embryo implantation...
2018: Cell Death Discovery
Marcel König, Daniel Siegmund, Lukasz J Raszeja, Aram Prokop, Nils Metzler-Nolte
Emerging resistances of tumors against multiple anti-cancer agents are a major concern in the chemotherapeutical treatment of various cancers. Clearly, this raises the need for novel therapeutics with new modes of action. Herein, we report on the favorable in vitro anti-proliferative properties of a phenanthridine-containing ReI (CO)3 complex (compound 1 , also abbreviated LR-166) and identify major contributions to its mode of action. The complex induces apoptosis in low micromolar concentrations even in drug-resistant Burkitt-like lymphoma (BJAB) and leukemia (Nalm-6) cell lines with known overexpression of p -glycoproteins as was confirmed by measuring the amount of hypodiploid DNA via FACS Scan analysis...
January 1, 2018: MedChemComm
Yaser Moghaddam, Alireza Andalib, Maryam Mohammad-Ganji, Vida Homayouni, Mohammadreza Sharifi, Mazdak Ganjalikhani-Hakemi
BACKGROUND: Acute Myeloid Leukemia (AML) is a Cancer of hematopoietic stem cells with a rapid progression. TIM-3 is expressed on leukemic stem cells (LSCs) in most types of AML and might have a positive effect on maintenance of malignant phenotype. MicroRNAs play important roles in either cancer progression or suppression. In this study were evaluated, the inhibitory effect of miR-498 on TIM-3 expression and its impact on proliferation and survival of HL-60 cell line. METHODS: Firstly, the probable inhibitory effect of miR-498 on TIM-3 expression was predicted...
July 24, 2018: Pathology, Research and Practice
Marlies Vanden Bempt, Sofie Demeyer, Michaël Broux, Jolien De Bie, Simon Bornschein, Nicole Mentens, Roel Vandepoel, Ellen Geerdens, Enrico Radaelli, Beat C Bornhauser, Andreas E Kulozik, Jules P Meijerink, Jean-Pierre Bourquin, Charles E de Bock, Jan Cools
The NUP214-ABL1 fusion is a constitutively activated tyrosine kinase that is significantly associated with overexpression of the TLX1 and TLX3 transcription factors in T cell acute lymphoblastic leukemia (T-ALL). Here we show that NUP214-ABL1 cooperates with TLX1 in driving T-ALL development using a transgenic mouse model and human T-ALL cells. Using integrated ChIP-sequencing, ATAC-sequencing, and RNA-sequencing data, we demonstrate that TLX1 and STAT5, the downstream effector of NUP214-ABL1, co-bind poised enhancer regions, and cooperatively activate the expression of key proto-oncogenes such as MYC and BCL2...
August 13, 2018: Cancer Cell
Elena Zerkalenkova, Svetlana Lebedeva, Anna Kazakova, Pavel Baryshev, Claus Meyer, Rolf Marschalek, Galina Novichkova, Michael Maschan, Aleksey Maschan, Yulia Olshanskaya
We present a leukemia case that exhibits a chromosomal translocation t(11;16)(q23;q23), which results in the expression of a novel KMT2A fusion gene. This novel fusion, KMT2A-USP10, was found in a relapse of acute myeloid leukaemia M5a. USP10 belongs to a protein family that deubiquitinates a distinct set of target proteins, and thus, increases the steady state protein levels of its target subproteome. One of the USP10 targets is TP53. Wildtype TP53 is usually rescued from proteasomal degradation by USP10. As most KMT2A leukemias display wildtype p53 alleles, one might argue that the disruption of an USP10 allele can be classified as a pro-oncogenic event...
August 14, 2018: Genes, Chromosomes & Cancer
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