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Clostridium butyricum;Fermentation

Surajit De Mandal, Sambanduram Samarjit Singh, Rajendra Bose Muthukumaran, Kawl Thanzami, Vinod Kumar, Nachimuthu Senthil Kumar
Fermented pork fat (sa-um) is traditionally and extensively consumed in Northeast Indian region for several decades. However, no scientific reports are available regarding its nutritional value as well as its potential health risks. The objective of this work was essentially the characterization of sa-um using a polyphasic approach, viz., physicochemical, electrospray ionization-mass spectrometry (ESI+ -MS) and metagenomic analysis in order to gain an understanding of the nutrient contents and microbial population diversity...
October 8, 2018: AMB Express
Jin-Jie Zhou, Jun-Tao Shen, Xiao-Li Wang, Ya-Qin Sun, Zhi-Long Xiu
Microbial consortium is an alternative for bioconversion of crude glycerol to value-added products whereas concerns about the process stability in long-term operation existed. The aim of this study is to evaluate the feasibility of using an anaerobic microbial consortium as inoculum for continuous conversion of crude glycerol to 1,3-propanediol (1,3-PDO). Performances of continuous fermentations with the consortium inoculum were evaluated under different dilution rates and glycerol feed concentrations. The highest 1,3-PDO production of 57...
July 26, 2018: Applied Microbiology and Biotechnology
Jong-Hun Park, Young-Bo Sim, Gopalakrishnan Kumar, Parthiban Anburajan, Jeong-Hoon Park, Hee-Deung Park, Sang-Hyoun Kim
This study investigated the kinetic parameters of high-rate continuous performance and biofilm layer formation in a H2 -producing dynamic membrane bioreactor, composed of a continuously stirred tank reactor along with an external module containing polyester mesh with a pore size of 100 µm. A maximum H2 production rate of 48.9 L/L-day and hydrogen yield of 2.8 mol/mol glucoseadded were attained at a hydraulic retention time of 3 h. The maximum specific growth rate and Monod constant were estimated as 14...
August 2018: Bioresource Technology
Tiago Pinto, Luísa Gouveia, Joana Ortigueira, Ganesh D Saratale, Patrícia Moura
In this work, hydrogen (H2 ) was produced through the fermentation of Spirogyra sp. biomass by Clostridium butyricum DSM 10702. Macronutrient stress was applied to increase the carbohydrate content in Spirogyra, and a 36% (w/w) accumulation of carbohydrates was reached by nitrogen depletion. The use of wet microalga as fermentable substrate was compared with physically and chemically treated biomass for increased carbohydrate solubilisation. The combination of drying, bead beating and mild acid hydrolysis produced a saccharification yield of 90...
August 2018: Journal of Bioscience and Bioengineering
Waraporn Apiwatanapiwat, Pilanee Vaithanomsat, Warunee Thanapase, Khanok Ratanakhanokchai, Akihiko Kosugi
Lignocellulosic biomass as co-substrate enhances the 1,3-propanediol (1,3-PD) production of anaerobic fermenters by increasing their conversion yield from glycerol. To improve 1,3-propanediol (1,3-PD) production by this efficient approach, Clostridium butyricum I5-42 was supplemented with lignocellulosic biomasses (starch free fiber (CPF) from cassava pulp and xylan) as co-substrates. The 1,3-PD production and growth of C. butyricum were considerably higher in glycerol plus CPF and xylan than in glycerol alone, whereas another major polysaccharide (cellulose co-substrate) failed to improve the 1,3-PD production...
June 2018: Journal of Bioscience and Bioengineering
Ai-Hui Zhang, Hao-Lin Liu, Shi-Yang Huang, You-Si Fu, Bai-Shan Fang
1,3-propanediol production by Clostridium butyricum is a low productivity process due to the long time seed cultivation and thus hinders its industrial scale production. In the present study, repeated batch fermentation coupled with activated carbon adsorption strategy was first established which conduced not only to saving the time of seed cultivation and enhancing the productivity, but also to reducing the costs for the seed cultivation to achieve the purpose of 1,3-propanediol continuous production. The concentration of 1,3-propanediol from first to fourth cycle was 42...
March 2018: Biotechnology and Bioengineering
Luis Miguel Serrano-Bermúdez, Andrés Fernando González Barrios, Costas D Maranas, Dolly Montoya
BACKGROUND: The increase in glycerol obtained as a byproduct of biodiesel has encouraged the production of new industrial products, such as 1,3-propanediol (PDO), using biotechnological transformation via bacteria like Clostridium butyricum. However, despite the increasing role of Clostridium butyricum as a bio-production platform, its metabolism remains poorly modeled. RESULTS: We reconstructed iCbu641, the first genome-scale metabolic (GSM) model of a PDO producer Clostridium strain, which included 641 genes, 365 enzymes, 891 reactions, and 701 metabolites...
June 1, 2017: BMC Systems Biology
Parthiban Anburajan, Jong-Hun Park, Periyasamy Sivagurunathan, Arivalagan Pugazhendhi, Gopalakrishnan Kumar, Chang-Su Choi, Sang-Hyoun Kim
This study examined the mesophilic continuous biohydrogen fermentation from galactose and glucose mixture with an initial substrate concentration of 15 g/L (galactose 12 g/L and glucose 3 g/L) as a resembling carbon source of pretreated red algal hydrolyzate. A fixed bed reactor was fed with the sugar mixture at various hydraulic retention times (HRTs) ranging 12 to 1.5 h. The maximum hydrogen production rate of 52.6 L/L-d was found at 2 h HRT, while the maximum hydrogen yield of 2.3±0.1 mol/mol hexoseadded, was achieved at 3 h HRT...
September 2017: Journal of Bioscience and Bioengineering
Jin-Jie Zhou, Jun-Tao Shen, Li-Li Jiang, Ya-Qin Sun, Ying Mu, Zhi-Long Xiu
Crude glycerol is an ideal feedstock for bioproduction of 1,3-propanediol (1,3-PDO) while pure culture always shows low substrate tolerance and limited productivity. In this study, an anaerobic microbial consortium for conversion of crude glycerol was selected and its 1,3-PDO production capacity was evaluated. The consortium was obtained from anaerobic activated sludge by 19 serial transfers and mainly consisted of 94.64% Clostridiaceae and 4.47% Peptostreptococcaceae. The consortium adapted well with high glycerol concentration of 120 g/L as well as wide substrate concentration fluctuation from 15 to 80 g/L, producing 60...
August 2017: Applied Microbiology and Biotechnology
Zhao Kang Tee, Jamaliah Md Jahim, Jian Ping Tan, Byung Hong Kim
Calcium carbonate was evaluated as a replacement for the base during the fermentation of glycerol by a highly productive strain of 1,3-propanediol (PDO), viz., Clostridium butyricum JKT37. Due to its high specific growth rate (µmax=0.53h(-1)), 40g/L of glycerol was completely converted into 19.6g/L of PDO in merely 7h of batch fermentation, leaving only acetate and butyrate as the by-products. The accumulation of these volatile fatty acids was circumvented with the addition of calcium carbonate as the pH neutraliser before the fermentation was inoculated...
June 2017: Bioresource Technology
Dan Jiang, Zhen Fang, Siew-Xian Chin, Xiao-Fei Tian, Tong-Chao Su
Biohydrogen production has received widespread attention from researchers in industry and academic fields. Response surface methodology (RSM) was applied to evaluate the effects of several key variables in anaerobic fermentation of glucose with Clostridium butyrium, and achieved the highest production rate and yield of hydrogen. Highest H2 yield of 2.02 mol H2/mol-glucose was achieved from 24 h bottle fermentation of glucose at 35 °C, while the composition of medium was (g/L): 15.66 glucose, 6.04 yeast extract, 4 tryptone, 3 K2HPO4, 3 KH2PO4, 0...
June 2, 2016: Scientific Reports
Waraporn Apiwatanapiwat, Pilanee Vaithanomsat, Chakrit Tachaapaikoon, Khanok Ratanakhanokchai, Akihiko Kosugi
To improve its 1,3-propanediol (1,3-PD) production, Clostridium butyricum was cultivated on glycerol medium supplemented with cassava pulp (CP). At small concentrations, the CP improved the 1,3-PD productivity of C. butyricum from (0.25±0.01)g/L/h (glycerol alone) to (0.43±0.02)g/L/h (glycerol+2g/L CP) after 24h fermentation.
July 20, 2016: Journal of Biotechnology
Chunjie Zhu, Baishan Fang, Shizhen Wang
The effects of culture conditions on the kinetic behavior of 1,3-propanediol (PD) fermentation were investigated with a kinetic model. First, with initial glycerol concentration (S0) increasing, μmax and PD inhibition increased. Glycerol assimilation was harder and a little glycerol was consumed on cell maintenance at high S0. Second, with yeast extract concentration increasing, PD inhibition decreased. However, μmax decreased and glycerol assimilation became harder. It seems that the stimulus effect of yeast extract resulted from decreased PD inhibition...
July 2016: Bioresource Technology
Matti S Kannisto, Rahul K Mangayil, Ankita Shrivastava-Bhattacharya, Brett I Pletschke, Matti T Karp, Ville P Santala
BACKGROUND: Pretreatment of lignocellulosic biomass can produce inhibitory compounds that are harmful for microorganisms used in the production of biofuels and other chemicals from lignocellulosic sugars. Selective inhibitor removal can be achieved with biodetoxification where microorganisms catabolize the inhibitors without consuming the sugars. We engineered the strictly aerobic Acinetobacter baylyi ADP1 for detoxification of lignocellulosic hydrolysates by removing the gene for glucose dehydrogenase, gcd, which catalyzes the first step in its glucose catabolism...
2015: Biotechnology for Biofuels
N Cassir, S Benamar, B La Scola
Clostridium butyricum, a strictly anaerobic spore-forming bacillus, is a common human and animal gut commensal bacterium, and is also frequently found in the environment. Whereas non-toxigenic strains are currently used as probiotics in Asia, other strains have been implicated in pathological conditions, such as botulism in infants or necrotizing enterocolitis in preterm neonates. In terms of the latter, within the same species, different strains have antagonist effects on the intestinal mucosa. In particular, short-chain fatty acids, which are products of carbohydrate fermentation, have a dose-dependent paradoxical effect...
January 2016: Clinical Microbiology and Infection
Daniela Bassi, Edoardo Puglisi, Pier Sandro Cocconcelli
The environment of hard cheese encourages bacterial synergies and competitions along the ripening process, which might lead in defects such as clostridial blowing. In this study, Denaturing Gradient Gel Electrophoresis (DGGE), a quantitative Clostridium tyrobutyricum PCR and next-generation Illumina-based sequencing of 16S rRNA gene were applied to study 83 Grana Padano spoiled samples. The aim was to investigate the community of clostridia involved in spoilage, the ecological relationships with the other members of the cheese microbiota, and the effect of lysozyme...
December 2015: Food Microbiology
Vinayak Laxman Pachapur, Saurabh Jyoti Sarma, Satinder Kaur Brar, Yann Le Bihan, Gerardo Buelna, Mausam Verma
Co-substrate utilization of various wastes with complementary characteristics can provide a complete medium for higher hydrogen production. This study evaluated potential of apple pomace hydrolysate (APH) co-fermented with crude glycerol (CG) for increased H2 production and decreased by-products formation. The central composite design (CCD) along with response surface methodology (RSM) was used as tool for optimization and 15 g/L of CG, 5 g/L of APH and 15% (v/v) inoculum were found to be optimum to produce as high as 26...
October 2015: Bioresource Technology
Peter Dürre
Clostridia are Gram-positive, anaerobic, endospore-forming bacteria, incapable of dissimilatory sulfate reduction. Comprising approximately 180 species, the genus Clostridium is one of the largest bacterial genera. Physiology is mostly devoted to acid production. Numerous pathways are known, such as the homoacetate fermentation by acetogens, the propionate fermentation by Clostridium propionicum, and the butyrate/butanol fermentation by C. acetobutylicum, a well-known solvent producer. Clostridia degrade sugars, alcohols, amino acids, purines, pyrimidines, and polymers such as starch and cellulose...
August 2014: Microbiology Spectrum
SangJoon Mo, Bong-Soo Kim, Sung-Jo Yun, Jung-Ju Lee, Suk-Hyun Yoon, Chung-Hun Oh
BACKGROUND: Clostridium butyricum is a butyric acid-producing anaerobic bacteriuma, and commonly present as gut microbiota in humans. This species has been used as a probiotic for the prevention of diarrhea in humans. In this study, we report the draft genome of C. butyricum DKU-01, which was isolated from infant feces, to better understand the characteristics of this strain so that it can later be used in the development of probiotic products. RESULTS: A total of 79 contigs generated by hybrid assembly of sequences obtained from Roche 454 and Illumina Miseq sequencing systems were investigated...
2015: Gut Pathogens
Magdalena Calusinska, Christopher Hamilton, Pieter Monsieurs, Gregory Mathy, Natalie Leys, Fabrice Franck, Bernard Joris, Philippe Thonart, Serge Hiligsmann, Annick Wilmotte
BACKGROUND: Molecular hydrogen, given its pollution-free combustion, has great potential to replace fossil fuels in future transportation and energy production. However, current industrial hydrogen production processes, such as steam reforming of methane, contribute significantly to the greenhouse effect. Therefore alternative methods, in particular the use of fermentative microorganisms, have attracted scientific interest in recent years. However the low overall yield obtained is a major challenge in biological H2 production...
2015: Biotechnology for Biofuels
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