Liqing Liang, Lei Xu, Qian Dong, Jing Zhang, Mingyi Qu, Xin Yuan, Quan Zeng, Huilin Li, Bowen Zhang, Chao Wang, Tao Fan, Li-Juan He, Wen Yue, Xiaoyan Xie, Xuetao Pei
Human pluripotent stem cell (hPSC)-derived red blood cells (RBCs) possess great potential for compensating shortages in transfusion medicine. For better RBC generation from hPSCs, we compared the cell seeding density in the embryoid body formation-based hPSC induction protocol. In the selection of low- and high-density inoculation conditions, we found that low-density culture performed better in the final RBC product with more cell output and increased average cellular hemoglobin content. An elaborate study using flow cytometry demonstrated that low inoculation density promoted endothelial-to-hematopoietic transition, followed by improved hematopoietic progenitor formation and erythrocyte generation...
April 13, 2024: Stem Cells and Development