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https://www.readbyqxmd.com/read/28221766/proteoform-profile-mapping-of-the-human-serum-complement-component-c9-reveals-unexpected-new-features-of-n-o-and-c-glycosylation
#1
Vojtech Franc, Yang Yang, Albert J R Heck
The human complement C9 protein (~65 kDa) is a member of the complement pathway. It plays an essential role in the membrane attack complex (MAC), which forms a lethal pore on the cellular surface of pathogenic bacteria. Here, we charted in detail the structural micro-heterogeneity of C9 purified from human blood serum, using an integrative workflow combining high-resolution native mass spectrometry and (glyco)peptide-centric proteomics. The proteoform profile of C9 was acquired by high-resolution native mass spectrometry, which revealed the co-occurrence of ~50 distinct MS signals...
February 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28220211/competitive-association-binding-kinetic-assays-a-new-tool-to-detect-two-different-binding-orientations-of-a-ligand-to-its-target-protein-under-distinct-conditions
#2
Hans-Joachim Wittmann, Andrea Strasser
Within the last years, for several ligands, binding to G protein-coupled receptors or other target proteins, a binding of the ligand in two different orientations is described. One appropriate experimental technique to detect two different binding orientations is the crystallization of the ligand-protein-complex, but crystallization and subsequent X-ray analysis do not belong to the routine methods. By traditional competitive radioligand equilibrium binding assays, it is not possible to detect or to distinguish between two different binding orientations, but there is a possibility to identify two different binding orientations by performing kinetic competitive radioligand-binding assays...
February 20, 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
https://www.readbyqxmd.com/read/28219457/maximum-time-to-rescue-after-the-1908-messina-reggio-calabria-earthquake-was-20-days-hints-for-disaster-planning
#3
Natale Gaspare De Santo, Carmela Bisaccia, Luca Salvatore De Santo
: Introduction Maximum time-to-rescue has been studied accurately for many earthquakes in the years 1985-2004. No study is available for historical quakes. Hypothesis/Problem This study aimed to evaluate long-term survivors (from the fifth day after the quake) of the Messina-Reggio Calabria earthquake (1908; Italy), which is considered, historically, to be the worst seismic event in Europe. METHODS: Accurate readings of 11 national newspapers from the fifth day after the quake looking for rescued persons and transferring, to an ad hoc form, all data relating to each rescued person...
February 21, 2017: Prehospital and Disaster Medicine
https://www.readbyqxmd.com/read/28219351/positive-bacterial-culture-in-conjunctival-sac-before-cataract-surgery-with-night-stay-is-related-to-diabetes-mellitus
#4
Tetsuhiro Kawata, Toshihiko Matsuo
BACKGROUND: The aim of this study is to elucidate background clinical factors in patients with positive bacterial culture for the conjunctival sac before cataract surgery in Japan. METHODS: Retrospective review was made on medical records of 576 consecutive patients who underwent conjunctival sac culture before cataract surgery with night stay at a hospital in 2 years from January 2013 to December 2014. In the patients with sequential bilateral surgeries, the data were chosen for bacterial culture in the eye which had earlier surgery...
February 20, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28218681/the-intra-s-checkpoint-responses-to-dna-damage
#5
REVIEW
Divya Ramalingam Iyer, Nicholas Rhind
Faithful duplication of the genome is a challenge because DNA is susceptible to damage by a number of intrinsic and extrinsic genotoxins, such as free radicals and UV light. Cells activate the intra-S checkpoint in response to damage during S phase to protect genomic integrity and ensure replication fidelity. The checkpoint prevents genomic instability mainly by regulating origin firing, fork progression, and transcription of G1/S genes in response to DNA damage. Several studies hint that regulation of forks is perhaps the most critical function of the intra-S checkpoint...
February 17, 2017: Genes
https://www.readbyqxmd.com/read/28216340/cellular-vacuolization-caused-by-overexpression-of-the-pikfyve-binding-deficient-vac14-l156r-is-rescued-by-starvation-and-inhibition-of-vacuolar-atpase
#6
Ulf Schulze, Beate Vollenbröker, Alexander Kühnl, Daniel Granado, Samet Bayraktar, Ursula Rescher, Hermann Pavenstädt, Thomas Weide
Phosphoinositides (PI) and converting enzymes are crucial determinants of organelle identity and morphology. One important endolysosomal specific PI is PI(3,5)P2, generated by the PIKfyve kinase, which orchestrates in combination with Vac14 and Fig4. Dysfunction of this complex leads to large intracellular vacuoles in various cell types and is linked to neurological diseases. Here, we characterize the vacuolization phenotype caused by overexpression of the PIKfyve binding deficient mutant Vac14(L156R) in podocytes, which represent specialized cells of the kidney...
February 16, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28214167/psychometric-evaluation-and-design-of-patient-centered-communication-measures-for-cancer-care-settings
#7
Bryce B Reeve, David M Thissen, Carla M Bann, Nicole Mack, Katherine Treiman, Hanna K Sanoff, Nancy Roach, Brooke E Magnus, Jason He, Laura K Wagner, Rebecca Moultrie, Kathryn D Jackson, Courtney Mann, Lauren A McCormack
OBJECTIVE: To evaluate the psychometric properties of questions that assess patient perceptions of patient-provider communication and design measures of patient-centered communication (PCC). METHODS: Participants (adults with colon or rectal cancer living in North Carolina) completed a survey at 2 to 3 months post-diagnosis. The survey included 87 questions in six PCC Functions: Exchanging Information, Fostering Health Relationships, Making Decisions, Responding to Emotions, Enabling Patient Self-Management, and Managing Uncertainty...
February 10, 2017: Patient Education and Counseling
https://www.readbyqxmd.com/read/28213543/functional-characterization-of-a-28-kb-catabolic-island-from-pseudomonas-sp-m1-involved-in-biotransformation-of-%C3%AE-myrcene-and-related-plant-derived-volatiles
#8
Pedro Soares-Castro, Pedro Montenegro-Silva, Hermann J Heipieper, Pedro M Santos
Pseudomonas sp. M1 is able to mineralize highly hydrophobic and recalcitrant compounds such as benzene, phenol and their methylated/halogenated derivatives, as well as the backbone of several monoterpenes. The ability to use such spectrum of compounds as sole carbon source is, most probably, associated with a genetic background evolved under different environmental constraints. The outstanding performance of M1 strain regarding β-myrcene catabolism was elucidated in this work, with focus on the biocatalytical potential of β-myrcene-associated core-code, comprised in a 28-kb genomic island (GI), predicted to be organized in 8 transcriptional units...
February 17, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28213343/checking-questionable-entry-of-personally-identifiable-information-encrypted-by-one-way-hash-transformation
#9
Xianlai Chen, Yang C Fann, Matthew McAuliffe, David Vismer, Rong Yang
BACKGROUND: As one of the several effective solutions for personal privacy protection, a global unique identifier (GUID) is linked with hash codes that are generated from combinations of personally identifiable information (PII) by a one-way hash algorithm. On the GUID server, no PII is permitted to be stored, and only GUID and hash codes are allowed. The quality of PII entry is critical to the GUID system. OBJECTIVE: The goal of our study was to explore a method of checking questionable entry of PII in this context without using or sending any portion of PII while registering a subject...
February 17, 2017: JMIR Medical Informatics
https://www.readbyqxmd.com/read/28213135/genetic-variants-in-the-peripheral-auditory-system-significantly-affect-adult-cochlear-implant-performance
#10
A Eliot Shearer, Robert W Eppsteiner, Kathy Frees, Viral Tejani, Christina M Sloan-Heggen, Carolyn Brown, Paul Abbas, Camille Dunn, Marlan R Hansen, Bruce J Gantz, Richard J H Smith
BACKGROUND: Cochlear implantation is an effective habilitation modality for adults with significant hearing loss. However, post-implant performance is variable. A portion of this variance in outcome can be attributed to clinical factors. Recent physiological studies suggest that the health of the spiral ganglion also impacts post-operative cochlear implant outcomes. The goal of this study was to determine whether genetic factors affecting spiral ganglion neurons may be associated with cochlear implant performance...
February 14, 2017: Hearing Research
https://www.readbyqxmd.com/read/28212843/natural-extranuclear-androgen-receptor-ligands-as-endocrine-disruptors-of-cancer-cell-growth
#11
Marilena Kampa, George Notas, Elias Castanas
Even though the term endocrine disruption primarily designates environmental chemicals that can interfere with the action of hormones, in recent years it has been extended to include also plant derived compounds that can reach the human body, naturally, or have been identified and studied as alternative pharmaceutical agents. In fact, for a large number of them, their antihormonal action was appreciated by different traditional herbal medicines. In the present review we report the majority of the plant derived compounds that exhibit an antiandrogenic effect and the known mechanisms of action...
February 14, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28211966/formation-of-organometallic-intermediate-states-in-on-surface-ullmann-couplings
#12
Dennis Barton, Hong-Ying Gao, Philipp Alexander Held, Armido Studer, Harald Fuchs, Nikos L Doltsinis, Johannes Neugebauer
Possible origins of the formation of organometallic intermediates in on-surface Ullmann couplings have been investigated by surface tunneling microscopy (STM) and density functional theory (DFT) calculations. We consider the case of iodobenzenes on the coinage metals Au, Ag and Cu. We found experimental evidence for the formation of surface vacancies and the presence of metal adatoms in these coupling reactions, which are taken as a hint for the reactive extraction of surface atoms by adsorbates. In a second step, we demonstrate by ab initio molecular dynamics calculations for aryl-iodides on copper that metal atoms can be pulled out of the surface to form metalorganic species...
February 17, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28210621/circular-rnas-in-cardiovascular-disease-an-overview
#13
REVIEW
Ximin Fan, Xinyu Weng, Yifan Zhao, Wei Chen, Tianyi Gan, Dachun Xu
Circular RNA (circRNA), a novel type of endogenous noncoding RNA (ncRNA), has become a research hotspot in recent years. CircRNAs are abundant and stably exist in creatures, and they are found with covalently closed loop structures in which they are quite different from linear RNAs. Nowadays, an increasing number of scientists have demonstrated that circRNAs may have played an essential role in the regulation of gene expression, especially acting as miRNA sponges, and have described the potential mechanisms of several circRNAs in diseases, hinting at their clinical therapeutic values...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28209609/pancreatic-cancer-progress-and-challenges-in-a-rapidly-moving-field
#14
Eric A Collisson, Kenneth P Olive
"Pancreatic Cancer: Advances in Science and Clinical Care," a Special Conference of the American Association for Cancer Research, was held in Orlando, FL, on May 12 to 15, bringing together more than 450 basic, translational, clinical, and epidemiologic pancreatic cancer researchers as well as pancreatic cancer patients, survivors, and advocates. Pancreatic cancer remains one of the great challenges in medicine, but the accelerating pace of research and early hints of clinical successes to come were palpable throughout the meeting...
February 16, 2017: Cancer Research
https://www.readbyqxmd.com/read/28209521/impaired-visual-competition-in-patients-with-homonymous-visual-field-defects
#15
A C Geuzebroek, A V van den Berg
Intense visual training can lead to partial recovery of visual field defects caused by lesions of the primary visual cortex. However, the standard visual detection and discrimination tasks, used to assess this recovery process tend to ignore the complexity of the natural visual environment, where multiple stimuli continuously interact. Visual competition is an essential component for natural search tasks and detecting unexpected events. Our study focused on visual decision-making and to what extent the recovered visual field can compete for attention with the 'intact' visual field...
February 13, 2017: Neuropsychologia
https://www.readbyqxmd.com/read/28207962/validating-an-electronic-health-literacy-scale-in-an-older-hispanic-population
#16
Judith Aponte, Kathleen M Nokes
AIM: Examine the validity of the Spanish version of an instrument used to measure electronic health literacy (eHEALS) with an older Hispanic population from a number of Spanish-language countries living in New York City in the United States (US). BACKGROUND: Although the internet is available globally, complex skills are needed in order to use this source of valuable health-related information effectively. Electronic health literacy is a multifactorial concept that includes health literacy but also requires technology skills...
February 16, 2017: Journal of Clinical Nursing
https://www.readbyqxmd.com/read/28206699/outer-sphere-2%C3%A2-e-2%C3%A2-h-transfer-reactions-of-ruthenium-ii-amine-and-ruthenium-iv-amido-complexes
#17
Mauricio Cattaneo, Scott A Ryken, James M Mayer
A diverse set of 2 e(-) /2 H(+) reactions are described that interconvert [Ru(II) (bpy)(en*)2 ](2+) and [Ru(IV) (bpy)(en-H*)2 ](2+) (bpy=2,2'-bipyridine, en*=H2 NCMe2 CMe2 NH2 , en*-H=H2 NCMe2 CMe2 NH(-) ), forming or cleaving different O-H, N-H, S-H, and C-H bonds. The reactions involve quinones, hydrazines, thiols, and 1,3-cyclohexadiene. These proton-coupled electron transfer reactions occur without substrate binding to the ruthenium center, but instead with precursor complex formation by hydrogen bonding...
February 16, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28205512/superplastic-behavior-of-silica-nanowires-obtained-by-direct-patterning-of-silsesquioxane-based-precursors
#18
Mustafa Yılmaz, Nicole Wollschläger, Mohammad Nasr Esfahani, Werner Österle, Yusuf Leblebici, B Erdem Alaca
Silica nanowires spanning 10 μm-deep trenches are fabricated from different types of silsesquioxane-based precursors by direct e-beam patterning on silicon followed by release through deep reactive ion etching. Nanowire aspect ratios as large as 150 are achieved with a critical dimension of about 50 nm and nearly rectangular cross-sections. In situ bending tests are carried out inside a scanning electron microscope, where the etch depth of 10 [Formula: see text] provides sufficient space for deformation. Silica NWs are indeed observed to exhibit superplastic behavior without fracture with deflections reaching the full etch depth, about two orders of magnitude larger than the nanowire thickness...
February 16, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28197537/fusion-rna-profiling-provides-hints-on-cell-of-origin-of-mysterious-tumor
#19
Zhongqiu Xie, Hui Li
Biological samples can be grouped into separate clusters based on their gene expression profiles. This approach has yielded meaningful biological insights and facilitated biomarker discoveries. Recently, we developed another approach to study connections between biological samples based on their fusion RNA expression. We have used this approach to provide insights into the cell of origin for a mysterious tumor, alveolar rhabdomyosarcoma.
2017: Molecular & Cellular Oncology
https://www.readbyqxmd.com/read/28196709/hybrid-aortic-arch-repair-facts-and-hints-beyond-the-statistical-truth
#20
EDITORIAL
Luca Salvatore De Santo
No abstract text is available yet for this article.
January 20, 2017: Journal of Thoracic and Cardiovascular Surgery
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