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circulating microRNA

Angelika Terbuch, Jan B Adiprasito, Verena Stiegelbauer, Maximilian Seles, Christiane Klec, Georg P Pichler, Margit Resel, Florian Posch, Anna L Lembeck, Herbert Stöger, Joanna Szkandera, Karl Pummer, Thomas Bauernhofer, Georg C Hutterer, Armin Gerger, Michael Stotz, Martin Pichler
Metastatic testicular germ cell tumors (TGCTs) are a potentially curable disease by administration of risk-adapted cytotoxic chemotherapy. Nevertheless, a disease-relapse after curative chemotherapy needs more intensive salvage chemotherapy and significantly worsens the prognosis of TGCT patients. Circulating tumor markers (β-subunit of human chorionic gonadotropin (β-HCG), alpha-Fetoprotein (AFP), and Lactate Dehydrogenase (LDH)) are frequently used for monitoring disease recurrence in TGCT patients, though they lack diagnostic sensitivity and specificity...
October 12, 2018: International Journal of Molecular Sciences
Claudia Cava, Ida Manna, Antonio Gambardella, Gloria Bertoli, Isabella Castiglioni
Epilepsy includes a group of disorders of the brain characterized by an enduring predisposition to generate epileptic seizures. Although familial epilepsy has a genetic component and heritability, the etiology of the majority of non-familial epilepsies has no known associated genetic mutations. In epilepsy, recent epigenetic profiles have highlighted a possible role of microRNAs in its pathophysiology. In particular, molecular profiling identifies a significant number of microRNAs (miRNAs) altered in epileptic hippocampus of both animal models and human tissues...
September 13, 2018: Molecular Therapy. Nucleic Acids
Ziba Nariman-Saleh-Fam, Milad Bastami, Mohammadreza Ardalan, Simin Sharifi, Seyed Mahdi Hosseinian Khatib, Sepideh Zununi Vahed
BACKGROUND: Chronic allograft dysfunction (CAD), the foremost cause of renal graft loss worldwide, is a serious challenge for most of the recipients. As the epigenetic era is emerging, epigenetic biomarkers especially microRNAs (miRNAs) may reflect the current stage of the disease and patient's therapy response. The current study investigated the potential significance of circulating miRNA-148a in predicting the renal graft function. DESIGN AND METHODS: Circulating miRNAs were isolated from 53 plasma samples of recipients with histologically validated interstitial fibrosis and tubular atrophy (IFTA, n = 26), and recipients with stable graft function (SGF, n = 27), and also healthy individuals ( n = 15)...
October 15, 2018: Journal of Cellular Biochemistry
Hui Wei, Ke Pu, Xiao-Guang Liu, Bo-Xuan Li, Heng-Shuo Zhang, Huan Wang, Hao Wang, Wei-Ming Sun, Yu-Ping Wang
Recently, cancer research microRNA studies have drawn great attention. However, the results of these studies have been inconsistent and variable regarding the availability of circulating miRNAs in gastric cancer (GC) diagnosis. Thus, results should be interpreted cautiously. The purpose of the present study was to assess the diagnostic performance of circulating miRNAs in GC diagnosis. We conducted a systematic and comprehensive approach for the inclusion of studies. The sensitivity, specificity, and diagnostic odds ratio were pooled with random effects models, and a summary of receiver operator characteristic (SROC) curves were plotted...
October 11, 2018: Oncology Reports
Tingting Sun, Lihua Zhang, Xinxin Li, Fengfei Chen, Yanchuan Li, Xiaohui Ma, Feng Yu
Aim: To investigate the protective effect of dantonic in ischemic myocardial damage by evaluating the expression of circulating microvesicles (MVs) and microRNA-1 (miR-1) in two animal models. Methods: Two animal models of myocardial ischemia were established that were isoproterenol-induced myocardial ischemia (ISO-AMI) rat model and the acute myocardial infarction rat model induced by ligation of the left anterior descending coronary artery (LAD-AMI) of rat. To investigate the protective effect of dantonic, we observed the myocardial infarction size, creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) activities, cardiac troponin I (cTnI) level in serum, and the plasma levels of miR-1 and MVs...
2018: Frontiers in Physiology
Hai-Ming Xu, Feng-Hua Sui, Mei-Hua Sun, Gong-Liang Guo
Recent studies have shown that circulating microRNAs (miRNA) play a critical role in diagnosing acute coronary syndrome (ACS). This study aims to investigate the effect of miR-224 on atherosclerotic plaques forming and vascular remodeling in ACS and its relationship with TGF-β/Smad pathway. Myocardial infarction (MI) rat model was established and lentivirus vector of miR-224 inhibitor was prepared for investigating the effect of downregulated miR-224 on the contents of nitric oxide (NO) and endothelin-1 (ET-1), blood lipid levels and inflammatory factor levels in serum as well as the TGF-β/Smad pathway...
October 14, 2018: Journal of Cellular Physiology
Liis Sabre, Jeffrey T Guptill, Melissa Russo, Vern C Juel, Janice M Massey, James F Howard, Lisa D Hobson-Webb, Anna Rostedt Punga
Muscle-specific tyrosine kinase antibody positive myasthenia gravis (MuSK+ MG) is an immunological subtype with distinctive pathogenic mechanisms and clinical features. The aim of this study was to analyze the circulating plasma microRNA profile of patients with MuSK+ MG. From the discovery cohort miR-210-3p, miR-324-3p and miR-328-3p were further analyzed in the validation cohort. We found a distinct plasma profile of miR-210-3p and miR-324-3p that were significantly decreased in MuSK+ MG patients compared to healthy controls (4...
October 6, 2018: Journal of Neuroimmunology
Janhavi M Modak, Meaghan Roy-O'Reilly, Liang Zhu, Ilene Staff, Louise D McCullough
BACKGROUND: MicroRNAs (miRNA) are a class of small, endogenous (17-25 nucleotide) noncoding ribonucleic acids implicated in the transcriptional and post-transcriptional regulation of gene expression. This study examines stroke-specific miRNA expression in large vessel territory cardioembolic stroke. METHODS: Peripheral blood was collected from controls and ischemic stroke patients 24hours after stroke onset. Whole blood miRNA was isolated and analyzed for differential expression...
October 10, 2018: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
David de Gonzalo-Calvo, Germán Cediel, Christian Bär, Julio Núñez, Elena Revuelta-Lopez, Josep Gavara, César Ríos-Navarro, Vicenta Llorente-Cortes, Vicente Bodí, Thomas Thum, Antoni Bayes-Genis
Reliable noninvasive prognostic biomarkers for left ventricular (LV) remodeling in ST-segment elevation myocardial infarction (STEMI) are needed. This study aimed to evaluate a panel of circulating microRNAs (miRNAs) as biomarkers of LV remodeling using cardiovascular magnetic resonance (CMR). We prospectively evaluated patients with a first STEMI treated with primary percutaneous coronary intervention who underwent CMR imaging at 1 week and 6 months after STEMI (n = 70). miRNAs were measured using PCR-based technologies in plasma samples collected at admission...
October 11, 2018: Scientific Reports
Hadeel A Al-Rawaf
BACKGROUND: Circulating microRNAs (miRNAs) as valuable biomarkers yielded important insights into the pathogenesis of obesity. AIM: This study aimed to describe the circulating miRNA profile for adolescences and its association with the circulating levels leptin and adiponectin according to specific degree of obesity. METHODS: RT-PCR and immunoassy analysis were used to study circulating miRNA profile, adipokines; adiponectin (A), leptin (L), and L/A ratio as well as other factors of metabolic syndrome (MS) in 250 adolescents with severe obesity...
September 25, 2018: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
Kyung Yoo, Ning Li, Vishruti Makani, Ravi Singh, Anthony Atala, Baisong Lu
microRNAs play important roles in physiologic and pathologic regulation, but their delivery for use in therapeutic applications is challenging. Extracellular vesicles (EVs), which are being explored as drug delivery vehicles, are stable in circulation and contain miRNAs. However, using EVs to deliver miRNAs has been limited by the difficulty of loading miRNAs into EVs. Here we describe a method to produce miRNA-enriched EVs in large quantities. We show that overexpressing a miRNA precursor in cells enormously increases the specific miRNA content in EVs...
October 11, 2018: Tissue Engineering. Part C, Methods
Xiangxiang Liu, Xiaoxiang Chen, Kaixuan Zeng, Mu Xu, Bangshun He, Yuqin Pan, Huiling Sun, Bei Pan, Xueni Xu, Tao Xu, Xiuxiu Hu, Shukui Wang
As one of the most common cancers worldwide, colorectal cancer (CRC) causes a large number of mortality annually. Aberrant expression of microRNAs (miRNAs) is significantly associated with the initiation and development of CRC. Further investigations regarding the regulatory mechanism of miRNAs is warranted. In this study, we discovered that miR-486-5p was remarkably downregulated in CRC, which partially results from higher DNA methylation in the promoter region detected by using methylation-specific PCR, bisulfite sequencing PCR, and DNA demethylation treatment...
October 10, 2018: Cell Death & Disease
Arash Latifkar, Richard A Cerione, Marc A Antonyak
Tumor cells interact with each other, and their surroundings, using a variety of mechanisms to promote virtually all aspects of cancer progression. One such form of intercellular communication that has been attracting considerable attention from the cancer community and the pharmaceutical industry in recent years involves the ability of cancer cells to generate multiple distinct types of non-classical secretory vesicles, generally referred to as extracellular vesicles (EVs). Microvesicles (MVs) represent one of the major classes of EVs and are formed as a result of the outward budding and fission of the plasma membrane...
October 8, 2018: Biochemical Society Transactions
Han-Byul Lee, Hyun-Kyu Park, Hyun-Ji Choi, Sora Lee, Sang-Joon Lee, Ji-Young Lee, Eun-Ho Cho, Hyo-Jeong Han, Ju-Hyung Seok, Woo-Chan Son
This study aimed to evaluate the usefulness of four microRNAs (miRNAs) in an acute pancreatic injury dog model. Acute pancreatitis was induced by infusion of cerulein for 2 h (7.5 μg/kg/h). The levels of well-known miRNAs, microRNA-216a (miR-216a) and microRNA-375 (miR-375), and new candidates microRNA-551b (miR-551b), and microRNA-7 (miR-7), were measured at 0, 0.5, 1, 2, 6, 12, and 24 h with serum amylase and lipase, and histopathological examination was performed. Among the four miRNAs, miR-216a and miR-375, and serum enzymes were significantly increased by cerulein treatment...
October 6, 2018: International Journal of Molecular Sciences
Ivana Sestak
PURPOSE OF REVIEW: There is growing consensus that genomic assays provide useful complementary information to clinicopathological features in oestrogen receptor (ER)-positive breast cancers. Here, ongoing research with multigene tests used for postmenopausal breast cancer and new emerging prognostic and predictive markers for pre- and postmenopausal women are summarised. RECENT FINDINGS: Results of the TAILORx trial have shown that women with an intermediate risk score do not benefit from adjuvant chemotherapy...
October 5, 2018: Current Opinion in Oncology
Janos Zempleni, Sonal Sukreet, Fang Zhou, Di Wu, Ezra Mutai
Exosomes are natural nanoparticles that play an important role in cell-to-cell communication. Communication is achieved through the transfer of cargos, such as microRNAs, from donor to recipient cells and binding of exosomes to cell surface receptors. Exosomes and their cargos are also obtained from dietary sources, such as milk. Exosome and cell glycoproteins are crucial for intestinal uptake. A large fraction of milk exosomes accumulates in the brain, whereas the tissue distribution of microRNA cargos varies among distinct species of microRNA...
October 4, 2018: Annual Review of Animal Biosciences
Masaaki Okamoto, Yoshimi Fukushima, Takahisa Kouwaki, Takuji Daito, Michinori Kohara, Hiroshi Kida, Hiroyuki Oshiumi
The innate immune system is important for the efficacy of vaccines, but excessive innate immune responses can cause adverse reactions after vaccination. Extracellular vesicles (EVs) are enriched in the blood and can deliver functional RNAs, such as microRNAs (miRNAs), to recipient cells, thereby mediating intercellular communication. However, the role of EVs in controlling the innate immune responses to vaccines has not been fully elucidated. Here, we found that miR-451a is abundant in human serum EVs and that its presence in blood-circulating EVs affects the innate immune responses of macrophages and dendritic cells to inactivated whole-virus vaccines (WV) against influenza...
October 3, 2018: Journal of Biological Chemistry
Yannan Fan, Maria Habib, Jianguo Xia
Xeno-miRNAs are microRNAs originating from exogenous species detected in host biofluids. A growing number of studies have suggested that many of these xeno-miRNAs may be involved in cross-species interactions and manipulations. To date, hundreds of xeno-miRNAs have been reported in different hosts at various abundance levels. Based on computational predictions, many more miRNAs could be potentially transferred to human circulation system. There is a clear need for bioinformatics resources and tools dedicated to xeno-miRNA annotations and their potential functions...
2018: PeerJ
M Laura Idda, Valeria Lodde, Waverly G McClusky, Jennifer L Martindale, Xiaoling Yang, Rachel Munk, Maristella Steri, Valeria Orrù, Antonella Mulas, Francesco Cucca, Kotb Abdelmohsen, Myriam Gorospe
Polymorphisms in untranslated regions (UTRs) of disease-associated mRNAs can alter protein production. We recently identified a genetic variant in the 3'UTR of the TNFSF13B gene, encoding the cytokine BAFF (B-cell-activating factor), that generates an alternative polyadenylation site yielding a shorter, more actively translated variant, BAFF-var mRNA. Accordingly, individuals bearing the TNFSF13B variant had higher circulating BAFF and elevated risk of developing autoimmune diseases. Here, we investigated the molecular mechanisms controlling the enhanced translation of BAFF-var mRNA...
October 1, 2018: Nucleic Acids Research
Manal S Fawzy, Baraah T Abu AlSel, Essam Al Ageeli, Saeed Awad Al-Qahtani, Mohamed M Abdel-Daim, Eman A Toraih
BACKGROUND: Circulating non-coding RNAs (ncRNAs) have been implicated in health and disease. This study aimed to evaluate the serum expression profile of microRNA-499a (miR-499a) and its selected bioinformatically predicted partner long-ncRNA MALAT1 (metastasis-associated lung adenocarcinoma transcript 1) in diabetes-related end-stage renal disease (ESRD) patients and to correlate the expressions with the patients' clinicolaboratory data. SUBJECTS AND METHODS: Real-time quantitative polymerase chain reaction was applied in diabetics with and without ESRD (n = 90 for each)...
September 29, 2018: Archives of Physiology and Biochemistry
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