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circulating microRNA

Ruixue Huang, Ting Yu, Ying Li, Jianan Hu
Background : Pulmonary fibrosis (PF) is a representative pathological change in patients with pneumoconiosis; however, due to the absence of reliable and early biomarkers, microRNAs have recently emerged as potential candidates for identification. Objectives : The aim of our study was to discover the potential of PF-specific circulating microRNAs as early biomarkers among patients with pneumoconiosis. Methods : Four dust-exposed patients with PF and four matched healthy individuals (not exposed to dust) were recruited for the study...
May 8, 2018: Toxicology Research
Gabriele Grunig, Christina A Eichstaedt, Jeremias Verweyen, Nedim Durmus, Stephanie Saxer, Greta Krafsur, Kurt Stenmark, Silvia Ulrich, Ekkehard Grünig, Serhiy Pylawka
Rationale: Therapeutic exercise training has been shown to significantly improve pulmonary hypertension (PH), including 6-min walking distance and right heart function. Supplemental nightly oxygen also has therapeutic effects. A biomarker tool that could query critical gene networks would aid in understanding the molecular effects of the interventions. Methods: Paired bio-banked serum ( n = 31) or plasma ( n = 21) samples from the exercise or oxygen intervention studies, respectively, and bio-banked plasma samples ( n = 20) from high altitude induced PH in cattle were tested...
2018: Frontiers in Physiology
Ammar Ebrahimi, Iraj Nikokar, Mehrdad Zokaei, Elahe Bozorgzadeh
The new sensitive diagnostic method, electrochemical nanobiosensor, is applied to cancer detection by using specific biomarkers such as miRNA. Studies have shown that for the diagnosis of Triple Negative Breast Cancer (TNBC) patients, in the early stages of the disease, miR-199a-5p as circulating miRNA can be a suitable candidate. In this study, a new electrochemical nanobiosensor for serum miR-199a-5p detection was designed by using modified glassy carbon electrode (GCE) with graphene oxide (GO) and gold nanoroad (GNR) on which a thiolated probe was immobilized...
November 1, 2018: Talanta
Alessia Finotti, Matteo Allegretti, Jessica Gasparello, Patrizio Giacomini, Demetrios A Spandidos, Giuseppe Spoto, Roberto Gambari
In oncology, liquid biopsy is used in the detection of next-generation analytes, such as tumor cells, cell-free nucleic acids and exosomes in peripheral blood and other body fluids from cancer patients. It is considered one of the most advanced non-invasive diagnostic systems to enable clinically relevant actions and implement precision medicine. Medical actions include, but are not limited to, early diagnosis, staging, prognosis, anticipation (lead time) and the prediction of therapy responses, as well as follow-up...
October 2018: International Journal of Oncology
Sergio Lario, Anna Brunet-Vega, María E Quílez, María J Ramírez-Lázaro, Juan J Lozano, Lorena García-Martínez, Carles Pericay, Mireia Miquel, Félix Junquera, Rafael Campo, Xavier Calvet
Background: Helicobacter pylori infection causes long-term chronic active gastritis, a risk factor for the intestinal and diffuse forms of gastric cancer. Most gastric cancers develop in a stepwise progression from chronic active gastritis to precursor lesions of gastric cancer. The early detection of gastric cancer improves survival. Studies with recent evidence have proposed circulating-microRNAs as biomarkers of cancer. Objective: The purpose of this study was to explore the circulating-microRNA profile from H...
June 2018: United European Gastroenterology Journal
Andreia M Silva, Maria I Almeida, José H Teixeira, Cristina Ivan, Joana Oliveira, Daniel Vasconcelos, Nuno Neves, Cláudia Ribeiro-Machado, Carla Cunha, Mário A Barbosa, George A Calin, Susana G Santos
Bone injury healing is an orchestrated process that starts with an inflammatory phase followed by repair and remodelling of the bone defect. The initial inflammation is characterized by local changes in immune cell populations and molecular mediators, including microRNAs (miRNAs). However, the systemic response to bone injury remains largely uncharacterized. Thus, this study aimed to profile the changes in the plasma miRnome after bone injury and determine its biological implications. Methods: A rat model of femoral bone defect was used, and animals were evaluated at days 3 and 14 after injury...
2018: Theranostics
Ewa Ł Stępień, Martyna Durak-Kozica, Agnieszka Kamińska, Marta Targosz-Korecka, Marcin Libera, Grzegorz Tylko, Agnieszka Opalińska, Maria Kapusta, Bogdan Solnica, Adriana Georgescu, Marina C Costa, Agnieszka Czyżewska-Buczyńska, Wojciech Witkiewicz, Maciej T Małecki, Francisco J Enguita
Ectosomes ( Ects ) are a subpopulation of extracellular vesicles formed by the process of plasma membrane shedding. In the present study, we profiled ectosome-specific microRNAs (miRNAs) in patients with type 2 diabetes mellitus (T2DM) and analyzed their pro- and anti-angiogenic potential. Methods: We used different approaches for detecting and enumerating Ects , including atomic force microscopy, cryogenic transmission electron microscopy, and nanoparticle tracking analysis. Furthermore, we used bioinformatics tools to analyze functional data obtained from specific miRNA enrichment signatures during angiogenesis and vasculature development...
2018: Theranostics
Isabelle Serr, Carolin Daniel
T follicular helper (TFH) cells are an integral part of humoral immunity by providing help to B cells to produce high-affinity antibodies. The TFH precursor compartment circulates in the blood and TFH cell dysregulation is implied in various autoimmune diseases including type 1 diabetes (T1D). Symptomatic T1D is preceded by a preclinical phase (indicated by the presence of islet autoantibodies) with a highly variable progression time to the symptomatic disease. This heterogeneity points toward differences in immune activation in children with a fast versus slow progressor phenotype...
2018: Frontiers in Immunology
Timothy Recaldin, Philip S Hobson, Elizabeth H Mann, Faruk Ramadani, David J Cousins, Paul Lavender, David J Fear
Class-switch recombination (CSR) is an essential B cell process that alters the isotype of antibody produced by the B cell, tailoring the immune response to the nature of the invading pathogen. CSR requires the activity of the mutagenic enzyme AID (encoded by AICDA) to generate chromosomal lesions within the immunoglobulin genes that initiate the class switching recombination event. These AID-mediated mutations also participate in somatic-hypermutation of the immunoglobulin variable region, driving affinity maturation...
August 3, 2018: Molecular Immunology
Tuba Gunel, Nilufer Kamali, Mohammad K Hosseini, Ece Gumusoglu, Ali Benian, Kilic Aydinli
PURPOSE: Preeclampsia (PE) is a pregnancy specific disease soon after 20 weeks of gestation where major symptoms are hypertension and proteinuria. The underlying pathology is believed to be abnormal placentation. Epigenetic and genetic factors have significant roles in abnormal placental development. MicroRNA's (miRNAs), being one of the most important epigenetic regulators, take part in abnormal placentation. Hsa-miR-195 is a molecule associated with abnormal placental growth mechanisms such as impaired cellular proliferation, inadequate trophoblastic invasion causing defective spiral artery remodeling and apoptosis...
August 5, 2018: Journal of Maternal-fetal & Neonatal Medicine
Tao Xu, Li Li, Hua-Qing Hu, Xiao-Ming Meng, Cheng Huang, Lei Zhang, Jian Qin, Jun Li
Alcoholic liver disease (ALD) is characterized by hepatocyte damage, inflammatory cell activation, and increased intestinal permeability leading to the clinical manifestations of alcoholic hepatitis. Selected members of the family of microRNAs (miRNAs) are affected by alcohol, resulting in an abnormal miRNA profile in the liver and circulation in ALD. Increasing evidence suggests that miRNAs that regulate inflammation, lipid metabolism and promote cancer are affected by excessive alcohol administration in mouse models of ALD...
August 4, 2018: Journal of Cellular Physiology
Chen-Chung Lin, Brandon F Law, Paul D Siegel, Justin M Hettick
BACKGROUND: Occupational exposure to the most widely used diisocyanate, 4,4'-methylene diphenyl diisocyanate (MDI), is a cause of occupational asthma (OA). Early recognition of MDI exposure and sensitization is essential for the prevention of MDI-OA. OBJECTIVE: Identify circulating microRNAs (miRs) as novel biomarkers for early detection of MDI exposure and prevention of MDI-OA. MATERIALS AND METHODS: Female BALB/c mice were exposed to one of three exposure regimens: dermal exposure to 1% MDI in acetone; nose-only exposure to 4580 ± 1497 μg/m3 MDI-aerosol for 60 minutes; or MDI dermal exposure/sensitization followed by MDI-aerosol inhalation challenge...
August 3, 2018: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
Lei Zhang, Yuan Zhang, Yanfang Zhao, Yu Wang, Han Ding, Sheng Xue, Peifeng Li
Coronary artery disease (CAD) contributes to a huge number of human death worldwide. The early diagnosis can arrest the development of CAD and effectively lower the mortality rate. Recently, circulating miRNAs emerged as CAD biomarkers. Area covered: Many efforts were paid to explore early diagnostic biomarkers of CAD. Some proteins have been used as diagnostic golden standard. However, the diagnostic and prognostic value of them is limited. MicroRNAs (miRNAs), a class of small noncoding RNAs, have been illustrated to regulate gene expression...
July 31, 2018: Expert Opinion on Therapeutic Patents
Ghada Al-Kafaji, Harun Muayad Said, Mahmood Abduljalil Alam, Ziad Tarraq Al Naieb
Prostate cancer (PCa) is the second most diagnosed malignancy, and the leading cause of cancer-associated mortality among males. Prostate-specific antigen (PSA) has long been used for the detection of PCa. However, PSA levels increase in PCa and benign prostatic hyperplasia (BPH), and are associated with a poor disease outcome. Circulating microRNAs (miRNAs) have been determined to be highly stable in the circulation, and could be utilized as biomarkers to improve disease diagnosis and management. In the present study, the effectiveness of four PCa-associated miRNAs in the discrimination of PCa from BPH and the risk-stratification of PCa was assessed...
July 2018: Oncology Letters
Takaaki Masuda, Yoshiaki Shinden, Miwa Noda, Hiroki Ueo, Qingjiang Hu, Yukihiro Yoshikawa, Yusuke Tsuruda, Yosuke Kuroda, Shuhei Ito, Hidetoshi Eguchi, Shinji Ohno, Koshi Mimori
BACKGROUND/AIM: Circulating microRNAs (miRs) in blood have been highlighted as diagnostic, prognostic and predictive biomarkers for "Precision medicine". This study aimed to explore the possibility of using circulating precursor miRs (pre-miRs) as clinical biomarkers of recurrence in breast cancer. MATERIALS AND METHODS: We performed miR microarray analyses of circulating miRs in blood from patients with or without recurrence of breast cancer and identified miR-488-5p as a recurrence-related miR...
August 2018: Anticancer Research
Jelena Brkić, Caroline Dunk, Jacob O'Brien, Guodong Fu, Lubna Nadeem, Yan-Ling Wang, David Rosman, Mohamed Salem, Oksana Shynlova, Issaka Yougbaré, Heyu Ni, Stephen J Lye, Chun Peng
Preeclampsia (PE) is the leading cause of maternal and neonatal morbidity and mortality. Defects in trophoblast invasion, differentiation of endovascular extravillous trophoblasts (enEVTs), and spiral artery remodeling are key factors in PE development. There are no markers clinically available to predict PE, leaving expedited delivery as the only effective therapy. Dysregulation of miRNA in clinical tissues and maternal circulation have opened a new avenue for biomarker discovery. In this study, we investigated the role of miR-218-5p in PE development...
July 12, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
Yu Wang, Eugenie R Lumbers, Anya L Arthurs, Celine Corbisier de Meaultsart, Andrea Mathe, Kelly A Avery-Kiejda, Claire T Roberts, Fiona Broughton Pipkin, Francine Z Marques, Brian J Morris, Kirsty G Pringle
STUDY QUESTION: Are any microRNAs (miRNAs) that target the placental renin-angiotensin system (RAS) in the human placenta suppressed in early gestation? SUMMARY ANSWER: Overall, 21 miRNAs with predicted RAS mRNA targets were less abundant in early versus term placentae and nine were more highly expressed. WHAT IS KNOWN ALREADY: Regulation of human placental RAS expression could alter placental development and therefore normal pregnancy outcome...
July 28, 2018: Molecular Human Reproduction
Evaldas Girdauskas, Johannes Petersen, Niklas Neumann, Martin Ungelenk, Ingo Kurth, Hermann Reichenspurner, Tanja Zeller
MicroRNAs (miRNAs) may serve as elegant tool to improve risk stratification in bicuspid aortic valve (BAV)-associated aortopathy. However, the exact pathogenetic pathway by which miRNAs impact aortopathy progression is unknown. Herewith, we aimed to analyze the association between circulating miRNAs and rare variants of aortopathy-related genes. 63 BAV patients (mean age 47.3±11.3 years, 92% male) with a root dilatation phenotype, who underwent aortic valve+/-proximal aortic surgery at a single institution (mean post-AVR follow-up 10...
2018: PloS One
D-S Wang, B Zhong, M-S Zhang, Y Gao
OBJECTIVE: Circulating microRNAs (miRNAs) have been reported as biomarkers for the early detection of colorectal cancer (CRC). We aimed at evaluating the diagnostic and prognostic value of serum miR-103 in CRC patients. PATIENTS AND METHODS: Quantitative reverse-transcription PCR (qRT-PCR) was applied to measure the miR-103 levels in blood samples of 96 patients and 60 controls. RESULTS: Our results demonstrated that serum miR-103 was overexpressed in CRC subjects and the receiver operating characteristic (ROC) curve analysis showed that serum miR-103 could differentiate CRC cases from controls with relatively high accuracy...
July 2018: European Review for Medical and Pharmacological Sciences
Saeid Afshar, Sepideh Afshar, Emily Warden, Hamed Manochehri, Massoud Saidijam
Background: The early diagnosis of colorectal cancer (CRC) is associated with improved survival rates, and development of novel non-invasive, sensitive, and specific diagnostic tests is highly demanded. The objective of this paper was to identify commonly circulating microRNA (miRNA) biomarkers for use in CRC diagnosis. Methods: For this purpose, an artificial neural network (ANN) model was proposed. Among miRNAs retrieved from the Gene Expression Omnibus dataset, four miRNAs with the best miRNA score were selected by ANN units...
July 30, 2018: Iranian Biomedical Journal
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