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circulating microRNA

Maria Assunta Piano, Lisa Gianesello, Angela Grassi, Paola Del Bianco, Adriana Mattiolo, Anna Maria Cattelan, Lolita Sasset, Paola Zanovello, Maria Luisa Calabrò
The aim of this study was to identify circulating microRNAs (miRNAs) that could be used as biomarkers in patients at risk for or affected by AIDS-Kaposi's sarcoma (KS). Screening of 377 miRNAs was performed using low-density arrays in pooled plasma samples of 10 HIV/human herpesvirus 8 (HHV8)-infected asymptomatic and 10 AIDS-KS patients before and after successful combined antiretroviral therapy (cART). MiR-375 was identified as a potential marker of active KS, being the most down-regulated in AIDS-KS patients after cART and the most up-regulated in naïve AIDS-KS patients compared to naïve asymptomatic subjects...
December 13, 2018: Journal of Cellular and Molecular Medicine
Wei Gao, Thian-Sze Wong, Ke-Xing Lv, Min-Juan Zhang, Raymond King-Yin Tsang, Jimmy Yu-Wai Chan
BACKGROUND: Nasopharyngeal carcinoma (NPC) latently infected by Epstein-Barr virus (EBV) expresses 40 EBV BART microRNAs (miRNAs). Difference in diagnostic efficacy of these miRNAs on NPC detection was observed. Here, we performed a comprehensive evaluation on the efficacy of these miRNAs. METHODS: Quantitative polymerase chain reaction was performed on plasma nucleic acid isolated from patients with NPC and noncancer donors. RESULTS: For primary NPC, BART2-5P, BART6-3P, BART7-3P, BART7-5P, BART9-5P, BART11-3P, BART17-5P, and BART19-5P were significantly elevated...
December 13, 2018: Head & Neck
Manabu Sakai, Bart Spee, Guy C M Grinwis, Louis C Penning, Monique E van Wolferen, Luc J W van der Laan, Hille Fieten
BACKGROUND: Chronic hepatitis (CH) in dogs is common and has the tendency to progress to liver cirrhosis (LC). Circulating microRNAs might have the potential as markers for disease progression. OBJECTIVES: To investigate whether concentration of specific microRNAs in serum correlate with the stage and grade of CH in Labrador Retrievers. ANIMALS: Twenty-two Labrador Retrievers with histological CH (n = 8), LC (n = 7), and normal liver (NL, n = 7)...
December 11, 2018: Journal of Veterinary Internal Medicine
Yun-Hui Wang, Yan-Wei Yin, Han Zhou, Yuan-Dong Cao
Early detection of nasopharyngeal carcinoma (NPC) is of vital importance for improving prognosis and survival rates. MicroRNA (miRNA) are a class of short and non-coding RNA molecules that are capable of inhibiting the translation of mRNA of target genes. Previous studies have revealed that miRNA are involved in tumorigenesis and cancer development. The RNase-resistance of circulating miRNA have made them valuable non-invasive biomarkers, and has therefore drawn particular attention to their therapeutic potential...
December 2018: Oncology Letters
Yanqing Zhang, Jiaqi Pan, Desheng Xu, Zhengkai Yang, Jingxue Sun, Lulu Sun, Yanmeizhi Wu, Hong Qiao
Circulating microRNAs (miRNAs) are promising diagnostic markers in various types of cancers, including papillary thyroid carcinoma (PTC). However, there is sparse information reported with regards to miRNA expression in papillary thyroid microcarcinoma (PTMC) or concerning the role of a combination of miRNAs and ultrasound (US) in the diagnosis of PTMC before surgery. Therefore, we designed a study that aimed to evaluate miRNA expression levels and their potential associations with US findings and determine whether miRNAs could be used as diagnostic and prognostic biomarkers of PTMC...
October 9, 2018: Oncology Reports
Kubíčková Adéla, Slabý Ondřej
BACKGROUND: A modern approach to identify biomarkers of solid cancers in tissues and body fluids is based on microRNA (miRNA) expression profiling. miRNAs are a group of approximately 3.000 short noncoding RNAs containing 18-25 nucleotides that regulate gene expression at the post-transcriptional (mRNA) level. The abilities of miRNAs to inhibit the translation or induce degradation of oncogenes and tumor suppressors indicate that they are involved in carcinogenesis. There is increasing evidence that miRNAs regulate apoptosis, cell proliferation, differentiation, and invasion...
2018: Klinická Onkologie: Casopis Ceské a Slovenské Onkologické Spolecnosti
Ju-Yi Li, Biao Cheng, Xiu-Fang Wang, Zhong-Jing Wang, Hong-Mei Zhang, Shu-Fen Liu, Li-Sha Chen, Wen-Jing Huang, Jue Liu, Ai-Ping Deng
Critical limb ischemia (CLI) is the most severe manifestation of peripheral artery disease, which is common but rarely diagnosed. Noninvasive biomarkers are urgently required to assist in the diagnosis of CLI. Accumulating evidence indicates that miRNAs play an important role in the development of various diseases. In this study, microarray profiling revealed 11 miRNAs with significantly altered expression in four T2DM patients with CLI compared with that in four sex- and age-matched T2DM patients without CLI...
2018: BioMed Research International
Anjui Wu, Gerhardt Attard
BACKGROUND: Molecular characterization of tumors could be important for clinical management. Plasma DNA obtained noninvasively as a liquid biopsy could be widely applicable for clinical implementation in biomarker-based treatment strategies. CONTENT: Prostate cancer is a disease with variable clinical outcomes and molecular features and therefore presents multiple opportunities for biomarker-based treatment optimization. Tissue analysis may not be representative of the lethal clone in localized disease or of intrapatient, intermetastases heterogeneity; fresh tissue is often challenging to obtain by biopsy of metastasis, whereas archival samples may not represent current disease and may be of insufficient quality...
December 11, 2018: Clinical Chemistry
Fernanda-Isadora Corona-Meraz, Mónica Vázquez-Del Mercado, Francisco José Ortega, Sandra-Luz Ruiz-Quezada, Milton-Omar Guzmán-Ornelas, Rosa-Elena Navarro-Hernández
OBJECTIVE: The identification of circulating microRNAs related to abnormal metabolic function may be useful in the context of ageing, adiposity and insulin resistance. The miR-33 a/b has been shown to control the expression of genes involved in fatty acid biosynthesis, impaired metabolism and insulin resistance. In this study, we aimed to identify differences in circulating miR-33 a/b levels according to age-related metabolic impairment and increased adiposity. METHODS: This study included 80 individuals (30...
December 11, 2018: Diabetes & Vascular Disease Research
Ahmed H Zedan, Torben F Hansen, Jannie Assenholt, Jonna S Madsen, Palle J S Osther
BACKGROUND: Overtreatment is a well-known clinical challenge in local prostate cancer (PCa). Although risk assessment models have contributed to a better stratification of patients with local PCa, a tailored management is still in its infancy. Over the last few decades, microRNAs (miRNAs) have shown promising results as biomarkers in PCa. The aim of this study was to investigate circulating miRNAs after management of local PCa. METHODS: The relative expression of four miRNAs (miRNA-21, -93, -125b, and miRNA-221) was assessed in plasma from 149 newly diagnosed patients with local or locally advanced PCa...
December 9, 2018: Prostate
Camilo Rojas, Elena Barnaeva, Ajit G Thomas, Xin Hu, Noel Southall, Juan Marugan, Amrita Datta Chaudhuri, Seung-Wan Yoo, Niyada Hin, Ondrej Stepanek, Ying Wu, Sarah C Zimmermann, Alexandra G Gadiano, Takashi Tsukamoto, Rana Rais, Norman Haughey, Marc Ferrer, Barbara S Slusher
Brain injury and inflammation induces a local release of extracellular vesicles (EVs) from astrocytes carrying proteins, RNAs, and microRNAs into the circulation. When these vesicles reach the liver, they stimulate the secretion of cytokines that mobilize peripheral immune cell infiltration into the brain, which can cause secondary tissue damage and impair recovery. Recent studies suggest that suppression of EV biosynthesis through neutral sphingomyelinase 2 (nSMase2) inhibition may represent a new therapeutic strategy...
December 7, 2018: Scientific Reports
Huiping Zhang, Jianfeng Wang, Zhanying Wang, Cailian Ruan, Lu Wang, Hongtao Guo
BACKGROUND: It is well known that some circulating microRNAs (miRNAs) are highly stable and might serve as promising biomarkers for many types of human cancer including glioblastoma (GBM). However, the potential clinical significance of serum miR-100 in GBM remained unknown. OBJECTIVE: We aimed to detect the expression level of serum miR-100 in patients with GBM and assess its potential diagnostic and prognostic value. METHODS: Quantitative real-time PCR was performed to measure serum miR-100 levels in 95 GBM patients and 60 healthy volunteers...
November 26, 2018: Cancer Biomarkers: Section A of Disease Markers
Pak Cheung Ng, Kathy Yuen Yee Chan, Terence Ping Yuen, Tony Sit, Hugh Simon Lam, Kam Tong Leung, Raymond Pui On Wong, Lawrence Chi Ngong Chan, Yennie Lap Ian Pang, Hon Ming Cheung, Winnie Chiu Wing Chu, Karen Li
OBJECTIVE: To discover specific circulating microRNA (miRNA) biomarkers for the early differentiation of necrotizing enterocolitis (NEC) from neonatal sepsis and inflammatory conditions. STUDY DESIGN: The study comprised 3 distinct phases: differential microarray analysis to compare plasma miRNA expression profiles of NEC vs sepsis and non-NEC/nonsepsis cases, a case-control study to quantify dysregulated miRNAs as potential specific biomarkers of NEC, and a prospective cohort study to assess the diagnostic usefulness of the best miRNA biomarker(s)...
October 24, 2018: Journal of Pediatrics
Francesco Sessa, Monica Salerno, Giulio Di Mizio, Giuseppe Bertozzi, Giovanni Messina, Benedetta Tomaiuolo, Daniela Pisanelli, Francesca Maglietta, Pietrantonio Ricci, Cristoforo Pomara
Even if anabolic androgenic steroid (AAS) abuse is clearly associated with a wide spectrum of collateral effects, adolescents and athletes frequently use a large group of synthetic derivatives of testosterone, both for aesthetic uses and for improving performance. Over the last few years, the development of MicroRNA (miRNA) technologies has become an essential part of research projects and their role as potential molecular biomarkers is being investigated by the scientific community. The circulating miRNAs detection as a diagnostic or prognostic tool for the diagnosis and treatment of several diseases is very useful, because with a minimal quantity of sample (peripheral blood), miRNAs are very sensitive...
2018: Frontiers in Pharmacology
Meizi Guo, Jun Luo, Junli Zhao, Dongya Shang, Qing Lv, Panpan Zang
BACKGROUND Circulating microRNAs (miRNAs) are emerging as novel biomarkers for detecting cardiovascular diseases. Here, circulating miR-133a and miR-221 were investigated as potential diagnostic biomarkers for heart failure (HF) patients, particularly in elderly patients. MATERIAL AND METHODS A total of 94 elderly HF patients (mean age=77.4 years old) and 31 healthy controls (age- and sex-matched) participated in this study. Plasma NT-proBNP levels were measured using an electrochemiluminescence immunoassay, and circulating miR-133a and miR-221 levels were examined using real-time quantitative PCR, with diagnostic efficacies determined for each independently and in combination...
December 7, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Steven Georges, Lidia Rodriguez Calleja, Camille Jacques, Melanie Lavaud, Brice Moukengue, Fernando Lecanda, Thibaut Quillard, Marta Tellez Gabriel, Pierre-François Cartron, Marc Baud'huin, François Lamoureux, Dominique Heymann, Benjamin Ory
The metastatic dissemination is a complex multistep process by which tumor cells from a primary site enter into the systemic circulation to finally spread at distant sites. Even if this mechanism is rare at the tumor level, it remains the major cause of Osteosarcoma-patients' relapse and mortality. MicroRNAs (miRNAs) have recently been described as novel epigenetics' genes' expression regulators actively implicated in cancer progression and dissemination. The understanding of their implication in the metastatic spreading could help clinicians to improve the outcome of osteosarcoma...
November 6, 2018: Oncotarget
Gary P Brennan, Dimitrios M Vitsios, Sophie Casey, Ann-Marie Looney, Boubou Hallberg, David C Henshall, Geraldine B Boylan, Deirdre M Murray, Catherine Mooney
MicroRNAs are a class of small non-coding RNA that regulate gene expression at a post-transcriptional level. MicroRNAs have been identified in various body fluids under normal conditions and their stability as well as their dysregulation in disease has led to ongoing interest in their diagnostic and prognostic potential. Circulating microRNAs may be valuable predictors of early-life complications such as birth asphyxia or neonatal seizures but there are relatively few data on microRNA content in plasma from healthy babies...
2018: PloS One
Ozge Caglar, Akin Cayir
BACKGROUND: Thyroid cancer is a common endocrine cancer. Great progress has been made in resolving its molecular mechanisms in recent years. The molecular changes observed in thyroid cancer can be used as biomarkers for diagnostic, prognostic and therapeutic purposes. MicroRNAs (miRNAs) are important components in biological and metabolic pathways, such as developmental stages, signal transduction, cell maintenance, and differentiation. Hence, their malfunctioning can expose humans to malignancies...
December 2, 2018: Journal of Cellular Biochemistry
Yury O Nunez Lopez, Ravi Retnakaran, Bernard Zinman, Richard E Pratley, Attila A Seyhan
OBJECTIVE: Short-term intensive insulin therapy (IIT) early in the course of type 2 diabetes acutely improves beta-cell function with long-lasting effects on glycemic control. However, conventional measures cannot determine which patients are better suited for IIT, and little is known about the molecular mechanisms determining response. Therefore, this study aimed to develop a model that could accurately predict the response to IIT and provide insight into molecular mechanisms driving such response in humans...
November 16, 2018: Molecular Metabolism
Emilie Dubois-Deruy, Marie Cuvelliez, Jan Fiedler, Henri Charrier, Paul Mulder, Eleonore Hebbar, Angelika Pfanne, Olivia Beseme, Maggy Chwastyniak, Philippe Amouyel, Vincent Richard, Christophe Bauters, Thomas Thum, Florence Pinet
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
November 29, 2018: Scientific Reports
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