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Brucella

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https://www.readbyqxmd.com/read/28437181/isolation-of-brucella-inopinata-like-bacteria-from-white-s-and-denny-s-tree-frogs
#1
Masanobu Kimura, Yumi Une, Michio Suzuki, Eun-Sil Park, Koichi Imaoka, Shigeru Morikawa
Brucella inopinata strain BO1 and B. sp. strain BO2 isolated from human patients, respectively, are genetically different from classical Brucella species. We isolated bacteria of the genus Brucella from two species of wild-caught tropical frogs kept in the facilities in Japan: White's tree frog, which inhabits Oceania, and Denny's tree frog, which inhabits Southeast Asia. Phylogenetic analyses based on 16S rRNA and recA gene sequences and multilocus sequence analysis showed that two isolates of Brucella spp...
February 16, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28432940/a-sandwich-immunoassay-for-brucellosis-diagnosis-based-on-immune-magnetic-beads-and-quantum-dots
#2
Li Li, Dehui Yin, Kun Xu, Yushen Liu, Dandan Song, Juan Wang, Chao Zhao, Xiuling Song, Juan Li
Brucellosis is a serious zoonosis with a rapid increase in incidence across epidemic regions. Currently, there are numerous methods for diagnosing brucellosis. However, these studies often have a few defects, such as low sensitivity, poor specificity, time consuming, laborious, and even potential biological risk. To overcome these shortcomings, we have developed a rapid, sensitive and accurate diagnosis procedure for brucellosis based on the immune magnetic beads (IMB) probe and quantum dots (QDs) - staphylococcal protein A (SPA) probe...
April 6, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28432327/genotyping-brucella-canis-isolates-using-a-highly-discriminatory-multilocus-variable-number-tandem-repeat-analysis-mlva-assay
#3
Yi Yang, Yin Wang, Elizabeth Poulsen, Russell Ransburgh, Xuming Liu, Baoyan An, Nanyan Lu, Gary Anderson, Chengming Wang, Jianfa Bai
Differentiation of Brucella canis from other Brucella species are mainly performed through PCR-based methods and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) procedures. Both PCR-based and MLVA methods are limited in discriminating B. canis strains. A new MLVA-13Bc method for B. canis genotyping was established by combining eight newly-developed VNTRs with five published ones. During 2010 and 2016, 377 B. canis PCR-positives were identified from 6,844 canine blood samples from 22 U.S. states, resulting in 229 B...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28418765/brucella-ceti-infection-in-a-common-minke-whale-balaenoptera-acutorostrata-with-associated-pathology
#4
Nicholas J Davison, Lorraine L Perrett, Claire Dawson, Mark P Dagleish, Gary Haskins, Jakub Muchowski, Adrian M Whatmore
There are three major lineages of marine mammal strains of Brucella spp.: Brucella ceti ST23, found predominantly in porpoises; B. ceti ST26, in pelagic delphinids and ziphiids; and Brucella pinnipedialis ST24/25, predominantly in seals. The isolation of Brucella spp. in mysticetes has been described only in common minke whales ( Balaenoptera acutorostrata ) in Norway and Scotland. We report a third case of Brucella infection and isolation in a minke whale associated with a large abscess. In contrast to the two previous reports that involved isolates of B...
April 18, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28417614/spinal-epidural-abscess-caused-by-brucella-species-a-review-of-17-cases
#5
Qin Zhang, Xiaohui Yang, Honglin Yu, Benrang Jia, Bin Meng
BACKGROUND: Spinal epidural abscess (SEA) is a rarely encountered clinical entity during the course of Brucella infection. METHODS: We reported 17 patients diagnosed with Brucellar SEA for a mean follow-up of 15 months. Spinal epidural abscess was detected by magnetic resonance imaging in all cases. Brucella diagnosis was established by serological tests. Patients were administered antibiotics for duration of 6 to 16 weeks. RESULTS: SEA was localized in lumbar region in 9 patients, thoracic in 4 patients, cervical in 3 patients, both thoracic and lumbar in 1 patient...
June 2017: Journal of Neurosurgical Sciences
https://www.readbyqxmd.com/read/28411163/development-of-a-multi-epitope-peptide-vaccine-inducing-robust-t-cell-responses-against-brucellosis-using-immunoinformatics-based-approaches
#6
Mahdiye Saadi, Ahmad Karkhah, Hamid Reza Nouri
Current investigations have demonstrated that a multi-epitope peptide vaccine targeting multiple antigens could be considered as an ideal approach for prevention and treatment of brucellosis. According to the latest findings, the most effective immunogenic antigens of brucella to induce immune responses are included Omp31, BP26, BLS, DnaK and L7-L12. Therefore, in the present study, an in silico approach was used to design a novel multi-epitope vaccine to elicit a desirable immune response against brucellosis...
April 11, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28408516/brucella-canis-in-a-dog-in-the-uk
#7
(no author information available yet)
No abstract text is available yet for this article.
April 15, 2017: Veterinary Record
https://www.readbyqxmd.com/read/28400761/brucella-genital-tropism-what-s-on-the-menu
#8
Jean-Jacques Letesson, Thibault Barbier, Amaia Zúñiga-Ripa, Jacques Godfroid, Xavier De Bolle, Ignacio Moriyón
No abstract text is available yet for this article.
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28398652/brucella-abortus-activated-microglia-induce-neuronal-death-through-primary-phagocytosis
#9
Ana M Rodríguez, M Victoria Delpino, M Cruz Miraglia, Miriam M Costa Franco, Paula Barrionuevo, Vida A Dennis, Sergio C Oliveira, Guillermo H Giambartolomei
Inflammation has long been implicated as a contributor to pathogenesis in neurobrucellosis. Many of the associated neurocognitive symptoms of neurobrucellosis may be the result of neuronal dysfunction resulting from the inflammatory response induced by Brucella abortus infection in the central nervous system. In this manuscript, we describe an immune mechanism for inflammatory activation of microglia that leads to neuronal death upon B. abortus infection. B. abortus was unable to infect or harm primary cultures of mouse neurons...
April 11, 2017: Glia
https://www.readbyqxmd.com/read/28398123/heterologous-complementation-reveals-a-specialized-activity-for-baca-in-the-medicago-sinorhizobium-meliloti-symbiosis
#10
George C diCenzo, Maryam Zamani, Hannah N Ludwig, Turlough M Finan
The bacterium Sinorhizobium meliloti Rm2011 forms N2-fixing root nodules on alfalfa and other leguminous plants. The pSymB chromid contains a 110-kb region (the ETR region) showing high synteny to a chromosomally located region in Sinorhizobium fredii NGR234 and related rhizobia. We recently introduced the ETR region from S. fredii NGR234 into the S. meliloti chromosome. Here, we report that, unexpectedly, the S. fredii NGR234 ETR region did not complement deletion of the S. meliloti ETR region in symbiosis with Medicago sativa...
April 10, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28391992/protein-protein-dna-dna-and-dna-protein-based-vaccination-strategies-using-truncated-omp2b-against-brucella-infection-in-balb-c-mice
#11
Maryam Golshani, Sima Rafati, Mehdi Nejati-Moheimani, Somaye Pourabdi, Amin Arsang, Saeid Bouzari
The purpose of the present study was to evaluate the immunogenicity and protective efficacy of the truncated form of outer membrane protein 2b (TOmp2b) from Brucella abortus in BALB/c mice. Three immunization regimens Protein/Protein, DNA/DNA and DNA/Protein were used. Immunization of mice with all vaccine strategies elicited a strong specific IgG responses (IgG2a titers over IgG1) and provided T helper1 (Th1) oriented immune responses. Furthermore, Protein/Protein (Pro/Pro-) and DNA/Pro- vaccinated groups conferred protection levels against B...
March 28, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28391650/identification-of-brucella-spp-in-feral-swine-sus-scrofa-at-abattoirs-in-texas-usa
#12
K Pedersen, N E Bauer, S Olsen, A M Arenas-Gamboa, A C Henry, T D Sibley, T Gidlewski
Various tissues, nasal swabs, urine and blood samples were collected from 376 feral swine at two federally inspected abattoirs in Texas during six separate sampling periods in 2015. Samples were tested for Brucella spp. by culture and serology. Brucella spp. were cultured from 13.0% of feral swine, and antibodies were detected in 9.8%. Only 32.7% of culture-positive feral swine were also antibody positive, and 43.2% of antibody-positive feral swine were culture positive. Approximately, the same number of males (14...
April 9, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28390976/rough-brucella-strain-rm57-is-attenuated-and-confers-protection-against-brucella-melitensis
#13
Yu Feng, Xiaowei Peng, Hui Jiang, Yong Peng, Liangquan Zhu, Jiabo Ding
Brucella melitensis (B. melitensis) is a facultative intracellular pathogen, which is the main epidemic strain in China. To overcome disadvantages of traditional live attenuated vaccines, in this study a rough mutant RM57 was induced from a B. melitensis isolate M1981. In order to uncover the reason of changes in the LPS of RM57, the nucleotide sequences and transcription levels of all known genes related to LPS synthesis were detected. As LPS plays an important role in outer membrane integrity, the sensitivities of RM57 to SDS and polymyxin B were examined...
April 5, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28389465/acquired-brucella-bacteraemia-in-a-young-infant
#14
Ambar Bhatnagar, Daisy Khera, Kuldeep Singh, Anuradha Sharma
An infant aged 6 weeks presented with failure to thrive since birth, fever and lethargy for 4 days. The child was fed on unpasteurised diluted goat's milk after birth. She was diagnosed to have brucellosis both by blood culture and serology. Her mother's Brucella serology was negative. She was successfully treated with injection amikacin and tablet rifampicin and had satisfactory weight gain thereafter. Acquired brucellosis should be considered in the differential diagnosis of failure to thrive in young infants...
April 7, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28388689/whole-genome-sequencing-of-brucella-melitensis-isolated-from-57-patients-in-germany-reveals-high-diversity-in-strains-from-middle-east
#15
Enrico Georgi, Mathias C Walter, Marie-Theres Pfalzgraf, Bernd H Northoff, Lesca M Holdt, Holger C Scholz, Lothar Zoeller, Sabine Zange, Markus H Antwerpen
Brucellosis, a worldwide common bacterial zoonotic disease, has become quite rare in Northern and Western Europe. However, since 2014 a significant increase of imported infections caused by Brucella (B.) melitensis has been noticed in Germany. Patients predominantly originated from Middle East including Turkey and Syria. These circumstances afforded an opportunity to gain insights into the population structure of Brucella strains. Brucella-isolates from 57 patients were recovered between January 2014 and June 2016 with culture confirmed brucellosis by the National Consultant Laboratory for Brucella...
2017: PloS One
https://www.readbyqxmd.com/read/28388625/poor-performance-of-the-rapid-test-for-human-brucellosis-in-health-facilities-in-kenya
#16
William A de Glanville, Raquel Conde-Álvarez, Ignacio Moriyón, John Njeru, Ramón Díaz, Elizabeth A J Cook, Matilda Morin, Barend M de C Bronsvoort, Lian F Thomas, Samuel Kariuki, Eric M Fèvre
Human brucellosis is considered to be an important but typically under-diagnosed cause of febrile illness in many low and middle-income countries. In Kenya, and throughout East Africa, laboratory diagnosis for the disease is based primarily on the febrile antigen Brucella agglutination test (FBAT), yet few studies of the diagnostic accuracy of this test exist. Assessment of the performance of the FBAT is essential for its appropriate clinical use, as well as for evaluating surveillance data reported by public health systems...
April 7, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28386600/novel-solutions-for-vaccines-and-diagnostics-to-combat-brucellosis
#17
Satadru Sekhar Mandal, Lucy Duncombe, N Vijaya Ganesh, Susmita Sarkar, Laurence Howells, Philip J Hogarth, David R Bundle, John McGiven
Brucellosis is diagnosed by detection of antibodies in the blood of animals and humans that are specific for two carbohydrate antigens, termed A and M, which are present concurrently in a single cell wall O-polysaccharide. Animal brucellosis vaccines contain these antigenic determinants, and consequently infected and vaccinated animals cannot be differentiated as both groups produce A and M specific antibodies. We hypothesized that chemical synthesis of a pure A vaccine would offer unique identification of infected animals by a synthetic M diagnostic antigen that would not react with antibodies generated by this vaccine...
March 22, 2017: ACS Central Science
https://www.readbyqxmd.com/read/28386446/early-animal-farming-and-zoonotic-disease-dynamics-modelling-brucellosis-transmission-in-neolithic-goat-populations
#18
Guillaume Fournié, Dirk U Pfeiffer, Robin Bendrey
Zoonotic pathogens are frequently hypothesized as emerging with the origins of farming, but evidence of this is elusive in the archaeological records. To explore the potential impact of animal domestication on zoonotic disease dynamics and human infection risk, we developed a model simulating the transmission of Brucella melitensis within early domestic goat populations. The model was informed by archaeological data describing goat populations in Neolithic settlements in the Fertile Crescent, and used to assess the potential of these populations to sustain the circulation of Brucella...
February 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28384918/an-uncommon-presentation-of-brucella-endocarditis-masquerading-as-neurobrucellosis
#19
Satish Venkata Pendela, Neha Agrawal, Thomas Mathew, Sudha Vidyasagar, Pujitha Kudaravalli
Brucella endocarditis is a rare but a severe complication of brucellosis, observed in less than 2% of cases. It is the main cause responsible for up to 80% of deaths in brucellosis. Herein, we present a case of brucella endocarditis that developed on a native aortic valve, but presented to us with fever for several months and acute neurological symptoms. This case report signifies the importance of considering brucella endocarditis as one of the differentials in patients presenting with Pyrexia of Unknown Origin (PUO) and Central Nervous System (CNS) manifestations...
February 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28384245/imported-human-brucellosis-in-belgium-bio-and-molecular-typing-of-bacterial-isolates-1996-2015
#20
Delphine Hanot Mambres, Samira Boarbi, Patrick Michel, Nora Bouker, Luisa Escobar-Calle, Damien Desqueper, Tiziano Fancello, Marjan Van Esbroeck, Jacques Godfroid, David Fretin, Marcella Mori
OBJECTIVES: The aim of this study was to characterize by classical biotyping and Multi-Locus variable number tandem repeats (VNTR) Analysis (MLVA) all Brucella spp. derived from human cases in Belgium from 1996 to 2015. Final goals were to determine the species and biovar, to trace-back on genetic grounds the origin of each strain when patient history and risk factors were missing, and to survey for particular trends at the national level. METHODS: A total of 37 Brucella strains, isolated from 37 patients in Belgium, were analyzed by both classical biotyping and MLVA, and the genetic patterns compared to those of human strains isolated worldwide...
2017: PloS One
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