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https://www.readbyqxmd.com/read/28734711/-bifidobacterium-scardovii-isolated-from-2-consecutive-urine-samples
#1
Daiana Guevara Nuñez, Adriana N De Paulis, Eugenia Bertona, Miguel Ángel Gutiérrez, Carlos A Vay, Juan P Suárez, Silvia C Predari
Bifidobacterium scardovii species consists of facultative anaerobic gram-positive rods whose growth is stimulated by CO2 and anaerobiosis. Exceptionally it has been associated with infections in humans. An elderly male patient with a urinary tract infection due to B. scardovii and Enterococcus faecalis is presented here; both microorganisms were isolated from two consecutive urine samples. The bacillus did not grow on standard media, but on chocolate agar incubated in CO2 and on supplemented Brucella agar in an anaerobic atmosphere, incubated for 72h at 35°C...
July 19, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28719613/visual-detection-of-brucella-in-bovine-biological-samples-using-dna-activated-gold-nanoparticles
#2
Dheeraj Pal, Nongthombam Boby, Satish Kumar, Gurpreet Kaur, Syed Atif Ali, Julien Reboud, Sameer Shrivastava, Praveen K Gupta, Jonathan M Cooper, Pallab Chaudhuri
Brucellosis is a bacterial disease, which, although affecting cattle primarily, has been associated with human infections, making its detection an important challenge. The existing gold standard diagnosis relies on the culture of bacteria which is a lengthy and costly process, taking up to 45 days. New technologies based on molecular diagnosis have been proposed, either through dip-stick, immunological assays, which have limited specificity, or using nucleic acid tests, which enable to identify the pathogen, but are impractical for use in the field, where most of the reservoir cases are located...
2017: PloS One
https://www.readbyqxmd.com/read/28717311/using-real-time-polymerase-chain-reaction-as-an-alternative-rapid-method-for-enumeration-of-colony-count-in-live-brucella-vaccines
#3
Waleed S Shell, Mahmoud L Sayed, A A Samy, Ghada Mohamed Al-Sadek, Gina Mohamed Mohamed Abd El-Hamid, Abdel Hakam M Ali
AIM: Brucellosis is a major bacterial zoonosis of global importance affecting a range of animal species and man worldwide. It has economic, public health, and bio-risk importance. Control and prevention of animal brucellosis mainly depend on accurate diagnostic tools and implementation of effective and safe animal vaccination program. There are three types of animal Brucella live vaccines - Brucella melitensis Rev-1 vaccine, Brucella abortus S19, and B. abortus RB51. Evaluation of these vaccines depends mainly on enumeration of Brucella viable count...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28715292/brucella-infection-in-asian-sea-otters-enhydra-lutris-lutris-on-bering-island-russia
#4
Tristan L Burgess, Christine Kreuder Johnson, Alexander Burdin, Verena A Gill, Angela M Doroff, Pamela Tuomi, Woutrina A Smith, Tracey Goldstein
Infection with Brucella spp., long known as a cause of abortion, infertility, and reproductive loss in domestic livestock, has increasingly been documented in marine mammals over the past two decades. We report molecular evidence of Brucella infection in Asian sea otters (Enhydra lutris lutris). Brucella DNA was detected in 3 of 78 (4%) rectal swab samples collected between 2004 and 2006 on Bering Island, Russia. These 78 animals had previously been documented to have a Brucella seroprevalence of 28%, markedly higher than the prevalence documented in sea otters (Enhydra lutris) in North America...
July 17, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28715291/isolation-of-brucella-pinnipedialis-from-grey-seals-halichoerus-grypus-in-the-baltic-sea
#5
Varpu Hirvelä-Koski, Minna Nylund, Teresa Skrzypczak, Petra Heikkinen, Kaarina Kauhala, Maryne Jay, Marja Isomursu
Brucella infection in seals was reported for the first time in 1994 around the coast of Scotland. Since then, marine mammal Brucella infections were found to be widely distributed in the northern hemisphere. Two Brucella species affect marine mammals: Brucella pinnipedialis in pinnipeds and Brucella ceti in cetaceans. We examined the livers of Baltic grey seals (Halichoerus grypus) from the Finnish coast (n=122) hunted, found dead, or killed as by-catch in fishing gear in 2013-15 as part of population health monitoring...
July 17, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28713359/exploring-the-diversity-of-field-strains-of-brucella-abortus-biovar-3-isolated-in-west-africa
#6
Moussa Sanogo, David Fretin, Eric Thys, Claude Saegerman
Brucellosis is one of the most widespread bacterial zoonotic diseases in the world, affecting both humans and domestic and wild animals. Identification and biotyping of field strains of Brucella are of key importance for a better knowledge of the epidemiology of brucellosis, for identifying appropriate antigens, for managing disease outbreaks and for setting up efficient preventive and control programmes. Such data are required both at national and regional level to assess potential threats for public health...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28713341/prevalence-host-range-and-comparative-genomic-analysis-of-temperate-ochrobactrum-phages
#7
Claudia Jäckel, Stefan Hertwig, Holger C Scholz, Karsten Nöckler, Jochen Reetz, Jens A Hammerl
Ochrobactrum and Brucella are closely related bacteria that populate different habitats and differ in their pathogenic properties. Only little is known about mobile genetic elements in these genera which might be important for survival and virulence. Previous studies on Brucella lysogeny indicated that active phages are rare in this genus. To gain insight into the presence and nature of prophages in Ochrobactrum, temperate phages were isolated from various species and characterized in detail. In silico analyses disclosed numerous prophages in published Ochrobactrum genomes...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28710229/pirate-project-point-of-care-informatics-based-randomised-controlled-trial-for-decreasing-overuse-of-antibiotic-therapy-in-gram-negative-bacteraemia
#8
Angela Huttner, Werner C Albrich, Pierre-Yves Bochud, Angèle Gayet-Ageron, Anne Rossel, Elodie von Dach, Stephan Harbarth, Laurent Kaiser
INTRODUCTION: Antibiotic overuse drives antibiotic resistance. The optimal duration of antibiotic therapy for Gram-negative bacteraemia (GNB), a common community and hospital-associated infection, remains unknown and unstudied via randomised controlled trials (RCTs). METHODS AND ANALYSIS: This investigator-initiated, multicentre, non-inferiority, informatics-based point-of-care RCT will randomly assign adult hospitalised patients receiving microbiologically efficacious antibiotic(s) for GNB to (1) 14 days of antibiotic therapy, (2) 7 days of therapy or (3) an individualised duration determined by clinical response and 75% reduction in peak C reactive protein (CRP) values...
July 13, 2017: BMJ Open
https://www.readbyqxmd.com/read/28710016/comparative-study-of-immunopathophysiological-responses-induced-by-b-%C3%A2-melitensis-and-its-lipopolysaccharide-in-mouse-model-infected-via-intranasal-route-of-exposure
#9
Abdinasir Yusuf Osman, Abdul Aziz Saharee, Faez Firdaus Jesse, Arifah Abdul Kadir
In this study, we developed a mouse model and characterized the effects of intranasal inoculation of virulent Brucella melitensis strain 16M and its lipopolysaccharide (LPS). The effects of the exposure were compared with respective control groups. Both Brucella melitensis-infected and LPS-infected groups showed no significant clinical presentation with minor relevance in the mortality associated with the infection. In Brucella melitensis-infected group, significant histopathological changes in comparison to the LPS infected group with increase bacterial burden in the lungs, reproductive and reticuloendothelial organs were observed...
July 11, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28702152/brucella-pericarditis-a-forgotten-cause-of-chest-pain
#10
Feridoun Sabzi, Reza Faraji
BACKGROUND: Brucellosis pericarditis is an extremely rare involvement of the cardiovascular system with Brucella species that has worldwide distribution and is endemic in many provinces of Iran. CASE PRESENTATION: The present report describes an exceptional case of pericardial involvement by Brucella melitensis in a 50- year-old woman. The patient presented with night sweats, mild fever, weight loss, and chest pain. CONCLUSION: Pericarditis occurs during the course of brucellosis as an isolated cardiac symptom and may be asymptomatic or may be manifested with chest pain as ischemic heart disease...
2017: Caspian Journal of Internal Medicine
https://www.readbyqxmd.com/read/28699872/in-vitro-synergistic-effects-of-a-short-cationic-peptide-and-clinically-used-antibiotics-against-drug-resistant-isolates-of-brucella-melitensis
#11
Zohreh Mohammadi Azad, Hoda Moravej, Mahdi Fasihi-Ramandi, Faramarz Masjedian, Raziyeh Nazari, Reza Mirnejad, Mehrdad Moosazadeh Moghaddam
PURPOSE: In the last few decades, increasing microbial resistance to common antibiotics has attracted researchers' attention to the development of new classes of antibiotics such as antimicrobial peptides. Accordingly, the aim of the current study was to evaluate antimicrobial effects of the CM11 peptide alone and combined with common antibiotics against drug-resistant isolates of Brucella melitensis. METHODOLOGY: A total of 50 pathogenic samples of B. melitensis were isolated from patients and their antibiotic susceptibility pattern was evaluated by E-test...
July 12, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28694807/brucella-omp25-upregulates-mir-155-mir-21-5p-and-mir-23b-to-inhibit-interleukin-12-production-via-modulation-of-programmed-death-1-signaling-in-human-monocyte-macrophages
#12
Beibei Cui, Wenli Liu, Xiaoya Wang, Yu Chen, Qian Du, Xiaomin Zhao, Hai Zhang, Shan-Lu Liu, Dewen Tong, Yong Huang
Brucella spp. infection results in compromised Type1 (Th1) cellular immune response. Several reports have described an immunomodulatory function for Brucella major outer membrane protein Omp25. However, the mechanism by which Omp25 modulates macrophage dysfunction has not been defined. Herein, we reported that Omp25-deficient mutant of Brucella suis exhibited an enhanced ability to induce interleukin (IL)-12 whereas ectopic expression of Omp25 protein inhibited TLR agonists-induced IL-12 p70 production through suppression of both IL-12 p40 and p35 subunit expression in THP-1 cells...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28693438/evaluation-of-recombinant-porin-romp2a-protein-as-a-potential-antigen-candidate-for-serodiagnosis-of-human-brucellosis
#13
Prachi Pathak, Ashu Kumar, Duraipandian Thavaselvam
BACKGROUND: Brucellosis is an important zoonotic disease caused by different Brucella species and human brucellosis is commonly prevalent in different states of India. Among various Brucella species, B. melitensis is most pathogenic to human and included as category B biothreat which can cause infection through aerosol, cut, wounds in skin and contact with infected animals. The diagnosis of human brucellosis is very important for proper treatment and management of disease as there is no vaccine available for human use...
July 11, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28693306/tannic-acid-mediated-immune-activation-attenuates-brucella-abortus-infection-in-mice
#14
Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Lauren Togonon Arayan, Tran Xuan Ngoc Huy, Wongi Min, Hu Jang Lee, Hong Hee Chang, Suk Kim
Brucellosis is an emerging infectious disease affecting humans and animals. In this study, we investigated the in vitro and in vivo effects of tannic acid (TA) against Brucella (B.) abortus infection. Reduced F-actin polymerization and MAPKs (ERK 1/2 and p38α) phosphorylation were observed in TA-treated cells as compared with control cells after infection. The mice were infected via intraperitoneal route and were orally given with TA or PBS for 14 days. The weights of the spleens from TA-treated and control mice were not different, however the splenic proliferation of B...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28692674/human-brucellosis-in-portugal-retrospective-analysis-of-suspected-clinical-cases-of-infection-from-2009-to-2016
#15
Ana Pelerito, Rita Cordeiro, Rita Matos, Maria Augusta Santos, Sofia Soeiro, João Santos, Carla Manita, Carla Rio, M Santo, Eleonora Paixão, Alexandra Nunes, Sofia Núncio
Brucellosis is a zoonosis that is emerging in some regions of the world. Although brucellosis is a disease of obligatory declaration and is not eradicated in Portugal, no prevalence data is available in this country. In this study, we retrospectively analyzed the data available at the Reference Laboratory at the Portuguese National Institute of Health during the past 7 years (2009-2016) in order to get insight into the epidemiological scenario of brucellosis in Portugal. A total of 2313 biological samples from patients with clinical suspicion of brucellosis were subjected to immunological techniques for laboratory diagnosis...
2017: PloS One
https://www.readbyqxmd.com/read/28691659/proline-utilization-system-is-required-for-infection-by-the-pathogenic-%C3%AE-proteobacterium-brucella-abortus
#16
Mitchell T Caudill, James A Budnick, Lauren M Sheehan, Christian R Lehman, Endang Purwantini, Biswarup Mukhopadhyay, Clayton C Caswell
Proline utilization (Put) systems have been described in a number of bacteria; however, the importance and functionality of the Put system in the intracellular pathogen Brucellaabortus has not been explored. Generally, bacterial Put systems are composed of the bifunctional enzyme proline dehydrogenase PutA and its transcriptional activator PutR. Here, we demonstrate that the genes putA (bab2_0518) and putR (bab2_0517) are critical for the chronic infection of mice by B. abortus, but putA and putR are not required for the survival and replication of the bacteria in naive macrophages...
July 8, 2017: Microbiology
https://www.readbyqxmd.com/read/28678344/prebiotic-potential-and-chemical-composition-of-seven-culinary-spice-extracts
#17
Qing-Yi Lu, Paula H Summanen, Ru-Po Lee, Jianjun Huang, Susanne M Henning, David Heber, Sydney M Finegold, Zhaoping Li
The objective of this study was to investigate prebiotic potential, chemical composition, and antioxidant capacity of spice extracts. Seven culinary spices including black pepper, cayenne pepper, cinnamon, ginger, Mediterranean oregano, rosemary, and turmeric were extracted with boiling water. Major chemical constituents were characterized by RP-HPLC-DAD method and antioxidant capacity was determined by measuring colorimetrically the extent to scavenge ABTS radical cations. Effects of spice extracts on the viability of 88 anaerobic and facultative isolates from intestinal microbiota were determined by using Brucella agar plates containing serial dilutions of extracts...
July 5, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28670646/serological-survey-of-toxoplasmosis-neosporosis-and-brucellosis-among-cattle-herds-in-oyo-state-south-western-nigeria
#18
Adekunle Ayinmode, Victor Akinseye, Gereon Schares, Simeon Cadmus
BACKGROUND: Several zoonotic diseases are known to constitute great impediment to livestock management and production worldwide, especially in developing countries where control measures are largely non-existent. This study sets out to investigate the occurrence of toxoplasmosis, neosporosis and brucellosis among cattle herds in Oyo State, southwest Nigeria. MATERIALS AND METHODS: A cross-sectional survey to screen for antibodies to Toxoplasma gondii, Neospora caninum and Brucella abortus was conducted among 174 cattle in 17 herds...
2017: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28669842/human-brucella-melitensis-infections-in-southern-vietnam
#19
EDITORIAL
James I Campbell, Nguyen Phu Huong Lan, Phan Minh Phuong, Le Buu Chau, Trung Pham Duc, Caterina Guzmán-Verri, Nazareth Ruiz-Villalobos, Tam Pham Thi Minh, Pilar M Muñoz Álvaro, Edgardo Moreno, Guy E Thwaites, Maia A Rabaa, Nguyen Van Vinh Chau, Stephen Baker
No abstract text is available yet for this article.
June 29, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28669636/occurrence-of-oxidative-stress-in-dairy-cows-seropositives-for-brucella-abortus
#20
Géssica Perin, Juscivete F Fávero, Diego R T Severo, Anielen D Silva, Gustavo Machado, Hugo L Araújo, Walter Lilenbaum, Vera M Morsch, Maria Rosa C Schetinger, Ricardo S Jordão, Lenita M Stefani, Nathieli B Bottari, Aleksandro S Da Silva
Bovine brucellosis is an important zoonotic disease caused by the bacterium Brucella abortus that leads to economic losses due to animal discard and commercial restrictions. Since positive animals for brucellosis are culled, little is known about the pathogenesis of this disease. Therefore, the aims of this study were to evaluate possible changes in the activity of deaminase adenosine (ADA) and the oxidative stress in cows seropositives for brucellosis (Experiment I), and to evaluate the seroprevalence of B...
June 29, 2017: Microbial Pathogenesis
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