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Brucella

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https://www.readbyqxmd.com/read/29345223/case-report-neurobrucellosis-with-plastered-spinal-arachnoiditis-a-magnetic-resonance-imaging-based-report
#1
Saraswati Nashi, Veeramani Preethish-Kumar, Sayani Maji, Nagarathna Chandrashekar, Kiran Polavarapu, Chetan Kashinkunti, Kajari Bhattacharya, Jitender Saini, Atchayaram Nalini
Diffuse spinal arachnoiditis in neurobrucellosis is a rare manifestation. We report a boy aged 17, presenting with hearing impairment and recurrent vomiting for 18 months, weight loss for 12 months, dysphagia, dysarthria, hypophonia for 6 months, and gait unsteadiness for 5 months. He had bilateral 5th (motor) to 12th cranial nerve palsy, wasting and weakness of limbs, fasciculations, absent tendon reflexes, and positive Babinski's sign. Cerebrospinal fluid (CSF) showed raised protein and pleocytosis. Magnetic resonance imaging (MRI) showed extensive enhancing exudates in cisterns and post-contrast enhancement of bilateral 5th, 6th, 7th, and 8th nerves...
January 15, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29344824/brucellosis-risk-in-urban-and-agro-pastoral-areas-in-tanzania
#2
Shingo Asakura, George Makingi, Rudovick Kazwala, Kohei Makita
Epidemiology of human and animal brucellosis may depend on ecological conditions. A cross-sectional study was conducted to compare prevalence and risk factors of bovine brucellosis, and risky behaviours for the human infection between urban and agro-pastoral areas in Morogoro region, Tanzania. Cattle blood sampling and interviews using a structured questionnaire were conducted with farmers. Rose-Bengal test was conducted for the cattle sera, and positive samples were confirmed with competitive ELISA. Farm-level sero-prevalences were 0...
January 17, 2018: EcoHealth
https://www.readbyqxmd.com/read/29338693/in-vitro-antimicrobial-susceptibility-testing-of-human-brucella-melitensis-isolates-from-ulanqab-of-inner-mongolia-china
#3
Zhi-Guo Liu, Dong-Dong Di, Miao Wang, Ri-Hong Liu, Hong-Yan Zhao, Dong-Ri Piao, Zhong-Zhi Zhao, Yong-Qing Hao, Ya-Nan Du, Hai Jiang, Bu-Yun Cui, Xian-Zhu Xia
BACKGROUND: Brucellosis is an endemic disease in the Inner Mongolia Autonomous Region of China and Ulanqab exhibits the highest prevalence of brucellosis in this region. Due to the complex nature of Brucellosis, a cure for this disease has proven to be elusive. Furthermore, the reduced susceptibility of Brucella spp. to antimicrobial agents has been reported as a potential cause of therapeutic failure. However, detailed in vitro antimicrobial susceptibility patterns pertaining to Brucella isolates from this region have not yet been published...
January 16, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29332311/otolaryngology-head-and-neck-region-manifestations-of-brucella
#4
Anna Lazutkin, Maya Korem, Jeffrey M Weinberger, Ron Eliashar, Nir Hirshoren
OBJECTIVES/HYPOTHESIS: Patients with Brucella infection present with nonspecific symptoms originating from different organs. In this study, we investigated the manifestations involving principally the otolaryngology/head and neck region. STUDY DESIGN: Retrospective cohort chart review. METHODS: A retrospective analysis of patients diagnosed with brucellosis in a tertiary medical center. Medical records of 55 patients treated for positive Brucella blood cultures between 2007 and 2016 were analyzed...
January 14, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29331367/molecular-characterization-and-differential-expression-analysis-of-interleukin-1%C3%AE-from-ovis-aries
#5
Yi-Ming Shui, Shi-Ying Lu, Xing Guo, Xi-Lin Liu, Bao-Quan Fu, Pan Hu, Lin-Lin Qu, Nan-Nan Liu, Yan-Song Li, Lu-Lu Wang, Fei-Fei Zhai, Dan-Di Ju, Zeng-Shan Liu, Yu Zhou, Hong-Lin Ren
The interleukin-1 family is an important component of the innate immune system and plays an important role in regulating immune responses on the invasion of intracellular parasites in the acquired immune system. Interleukin 1β (IL-1β) is one of the members of the IL-1 family that predominantly activates downstream signaling pathways to play immunological functions of stimulating T and B lymphocyte activation and promoting the various syntheses of inflammatory substances in conjunction with other cytokines...
January 10, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29329460/survey-of-parasitic-bacteria-in-bat-bugs-colorado
#6
Clifton D McKee, Lynn M Osikowicz, Teresa R Schwedhelm, Ying Bai, Kevin T Castle, Michael Y Kosoy
Bat bugs (Cimex adjunctus Barber) (Hemiptera: Cimicidae) collected from big brown bats (Eptesicus fuscus Palisot de Beauvoir) in Colorado, United States were assessed for the presence of Bartonella, Brucella, and Yersinia spp. using molecular techniques. No evidence of Brucella or Yersinia infection was found in the 55 specimens collected; however, 4/55 (7.3%) of the specimens were positive for Bartonella DNA. Multi-locus characterization of Bartonella DNA shows that sequences in bat bugs are phylogenetically related to other Bartonella isolates and sequences from European bats...
January 10, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29329166/hematologic-manifestations-of-brucellosis-in-children
#7
Naphtali Justman, Yariv Fruchtman, David Greenberg, Ben-Shimol Shalom
BACKGROUND: Brucellosis is a common zoonosis in the Bedouin population of southern Israel. Limited data exist for the rate and risk factors of hematologic complication of brucellosis in children. We assessed anemia, leukopenia, thrombocytopenia and pancytopenia in childhood brucellosis in southern Israel. METHODS: Our medical center is the sole hospital in southern-Israel. All medical files of brucellosis, 2005-2014, identified through positive blood cultures or International Classification of Diseases 9 revision (ICD9) coding with positive serology, were reviewed retrospectively...
January 11, 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29326214/whole-genome-sequences-of-brucella-melitensis-strain-qh61-isolated-from-yak-in-qinghai-china
#8
Xiaoan Cao, Zhaocai Li, Zhongzi Lou, Baoquan Fu, Yongsheng Liu, Youjun Shang, Jizhang Zhou, Zhizhong Jing
The facultative intracellular Gram-negative bacterium Brucella melitensis causes brucellosis in domestic and wild mammals. Brucella melitensis QH61 was isolated from a yak suffering from abortion in 2015 in Qinghai, China. Here, we report the whole-genome sequence of B. melitensis strain QH61.
January 11, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29325043/roma-a-periplasmic-protein-involved-in-the-synthesis-of-the-lipopolysaccharide-tunes-down-the-inflammatory-response-triggered-by-brucella
#9
Ezequiel Valguarnera, Juan M Spera, Cecilia Czibener, Fabiana R Fulgenzi, Adriana C Casabuono, Silvia G Altabe, Karina A Pasquevich, Francisco Guaimas, Juliana Cassataro, Alicia S Couto, Juan E Ugalde
Brucellaceae are stealthy pathogens with the ability to survive and replicate in the host in the context of a strong immune response. This capacity relies on several virulence factors that are able to modulate the immune system, and in their structural components that have low pro-inflammatory activities. Lipopolysaccharide (LPS), the main component of the outer membrane, is a central virulence factor of Brucella and it has been well established that induces a low inflammatory response. We describe here the identification and characterization of a novel periplasmic protein (RomA) conserved in alpha-proteobacteria, involved in the homeostasis of the outer membrane...
January 6, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29322764/rapid-veterinary-diagnosis-of-bovine-reproductive-infectious-diseases-from-semen-using-paper-origami-dna-microfluidics
#10
Zhugen Yang, Gaolian Xu, Julien Reboud, Syed Atif Ali, Gurpreet Kaur, John McGiven, Nongthombam Boby, Praveen Gupta, Pallab Chaudhuri, Jonathan Mark Cooper
The health and well-being of cattle is a significant concern for global agricultural output. In dairy production within low and middle income countries (LMICs), there is a significant biosensing challenge in detecting sexually transmitted infection (STI) pathogens during animal husbandry, due in part to difficulties associated with the limited infrastructure for veterinary medicine. Here we demonstrate low-cost, multiplexed and sample-to-answer paper-origami tests for the detection of three bovine infectious reproductive diseases in semen samples, collected at a test site in rural India...
January 11, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29319583/the-infectious-and-non-infectious-etiology-clinical-picture-and-outcome-of-neutropenia-in-immunocompetent-hospitalized-children
#11
Odeya David, Yariv Fruchtman, Ruslan Sergienko, Joseph Kapelushnik, Eugene Leibovitz
BACKGROUND: Acquired neutropenia in immunocompetent children is common and its differential diagnosis ranges from benign causes to life-threatening diseases. We described the etiology, clinical picture and outcome of new onset neutropenia in immunocompetent children assessed in the emergency department and hospitalized at our medical center. PATIENTS AND METHODS: Previously healthy children admitted with neutropenia (absolute neutrophil count [ANC] <1.5 x 10/L) were included...
January 9, 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29316759/-investigation-on-exposure-and-protection-of-brucella-in-animal-epidemic-prevention-institutions-in-a-city-of-ningxia
#12
(no author information available yet)
No abstract text is available yet for this article.
November 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/29313337/improved-reversible-cross-linking-based-solid-phase-rna-extraction-for-pathogen-diagnostics
#13
Fei Zhao, Eun Yeong Lee, Yong Shin
In this study, we developed an amine-functionalized, diatomaceous earth-based, dimethyl suberimidate assisted (ADD) system as a novel binding strategy to improve solid-phase extraction method for rapid and simple purification of RNA from biological samples including human cells and pathogenic bacteria. This ADD system is based on reversible cross-linking reactions between RNA and the silica matrix. The formation of robust covalent bonds protects RNA from both the sufferance of washing steps and isolation with ribonuclease (RNase)-rich samples, leading to the extraction of higher quality RNA...
January 9, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29310845/serological-study-of-brucellosis-in-argentine-creole-sheep
#14
Gustavo E López, Sabrina Peña, Gabriela I Escobar, Déborah B Hasan, Nidia E Lucero
Ovine cattle was introduced into America during the Spanish conquest with the second journey of Columbus to the Antilles and was disseminated throughout the region. In 1587, sheep were introduced into Argentina, later developing into the "Creole" breed. We selected 486 animals from different Argentine provinces with the aim of determining the serological status of brucellosis caused by Brucella melitensis and Brucella ovis. For the detection of antibodies against smooth Brucella spp., the Rose Bengal test (RBT) was performed as screening test while the serum agglutination test (SAT) and 2 mercapto-ethanol (2ME) were run as a confirmatory technique...
January 5, 2018: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29305897/cloning-expression-and-purification-of-virb10-protein-of-brucella-melitensis-and-to-evaluate-its-role-as-a-serological-marker-for-brucella-infection-in-experimental-and-natural-hosts
#15
Prachi Pathak, Ashu Kumar, Prabhu Prasad Sarangi, Sameer Bhagyawant, Duraipandian Thavaselvam
Brucellosis is a zoonotic disease caused by various species of the genus Brucella. The control of disease mainly depends on its accurate and early diagnosis. Culture methods employed for diagnosis are time consuming and require well equipped biosafety level 3 laboratories and hence serological tests are favored alternative for brucellosis diagnosis. At present serological diagnosis is based on LPS (lipopolysaccharide) which is less specific as the LPS antigen of Brucella species shows cross reactivity with other gram-negative bacteria...
January 3, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29302824/brucella-abortus-phosphoglyceromutase-and-dihydrodipicolinate-reductase-induce-th1-and-th2-related-immune-responses
#16
Zhiqiang Li, Hui Zhang, Jinliang Zhang, Li Xi, Guangli Yang, Shuli Wang, Qingfeng Zhou, Xiaogen Zhang, Junbo Zhang
Brucellae are intracellular bacterial pathogens that cause Brucellosis, bringing great economic burdens to developing countries. The pathogenic mechanisms of Brucella are still poorly understood. Earlier immune response plays an important role in the Brucella infection. Phosphoglyceromutase (PGM) and dihydrodipicolinate reductase (DapB) were cloned, expressed, purified, and their immunocompetence was analyzed. Cytokines were detected by murine macrophages (RAW 264.7) and splenocytes that stimulated with the two recombinant proteins...
January 4, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29302106/brucella-canis-an-update-on-research-and-clinical-management
#17
REVIEW
Kevin L Cosford
In Canada, Brucella canis remains a potentially devastating infectious agent that is still considered uncommon, despite the increasing international movement of dogs. There may be a growing risk to the Canadian canine population due to a reliance on outdated seroprevalence studies and the lack of federal regulation. With the complex diagnostic and management challenges associated with Brucella canis, a One Health approach is necessary to address the need for ongoing research, including updating canine and human seroprevalence rates in Canada, elucidating the pathogenesis, and determining the most appropriate treatment and prevention strategies...
January 2018: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
https://www.readbyqxmd.com/read/29301939/interleukin-10-suppresses-lysosome-mediated-killing-of-brucella-abortus-in-cultured-macrophages
#18
Huynh Tan Hop, Alisha Wehdnesday Bernardo Reyes, Tran Xuan Ngoc Huy, Lauren Togonon Arayan, WonGi Min, Hu Jang Lee, Man Hee Rhee, Hong Hee Chang, Suk Kim
Brucella abortus is a Gram-negative zoonotic pathogen for which there is no 100% effective vaccine. Phagosomes in B. abortus-infected cells fail to mature allowing the pathogen to survive and proliferate. Interleukin 10 (IL-10) promotes B. abortus persistence in macrophages by mechanisms that are not fully understood. In this study, we investigated the regulatory role of IL-10 in the immune response to B. abortus infection. B. abortus-infected macrophages were treated with either IL10 siRNA or recombinant IL-10 (rIL-10) and the expression of phagolysosome- or inflammation-related genes was evaluated by qRT-PCR and Western blot...
January 4, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29289485/a-microfluidic-enrichment-platform-with-a-recombinase-polymerase-amplification-sensor-for-pathogen-diagnosis
#19
Dao Thi Thuy Nguyen, Eun Yeong Lee, Bonhan Koo, Choong Eun Jin, Tae Yoon Lee, Yong Shin
Rapid and sensitive detection of low amounts of pathogen in large samples is needed for early diagnosis and treatment of patients and surveillance of pathogen. In this study, we report a microfluidic platform for detection of low pathogen levels in a large sample volume that couples an Magainin 1 based microfluidic platform for pathogen enrichment and a recombinase polymerase amplification (RPA) sensor for simultaneous pathogenic DNA amplification and detection in a label-free and real-time manner. Magainin 1 is used as a pathogen enrichment agent with a herringbone microfluidic chip...
December 28, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29280085/usefulness-of-a-single-assay-chemiluminescence-test-tularaemia-virclia-igg%C3%A2-%C3%A2-igm-monotest-for-the-diagnosis-of-human-tularemia-comparison-of-five-serological-tests
#20
África Cubero, Carlos Durántez, Ana Almaraz, Luis Fernández-Lago, María P Gutiérrez, María J Castro, Miguel A Bratos, María Simarro, Gabriel A March, Antonio Orduña
The aim of this work was to ascertain the usefulness of a new commercially-available single-assay chemiluminescence test (CHT) for the diagnosis of human tularemia (Tularaemia VIRCLIA IgG + IgM monotest, Vircell, Santa Fe, Granada, Spain). A total of 773 sera from 773 patients including 364 initial sera from patients with diagnosed tularemia, patients with suspected tularemia not confirmed (100), healthy people (152), patients with serology positive to Brucella (97), patients diagnosed with other infectious diseases (30), and patients diagnosed with autoimmune diseases (30) were included...
December 27, 2017: European Journal of Clinical Microbiology & Infectious Diseases
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