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https://www.readbyqxmd.com/read/28345992/evaluation-of-a-hypervariable-octameric-oligonucleotide-fingerprints-assay-for-identification-of-and-discrimination-between-wild-type-and-vaccine-strains-of-brucella-melitensis
#1
Lijuan Tang, Jun Liu, Yuanzhi Wang, Hui Zhang, Chuangfu Chen
OBJECTIVE To evaluate a hypervariable octameric oligonucleotide fingerprints (HOOF-Prints) assay for identification of and discrimination between wild-type and vaccine strains of Brucella melitensis. SAMPLE Brucella melitensis vaccine strain M5 and wild-type strain M43. PROCEDURES 8 pairs of primers (alterable, octameric nucleotides) were designed on the basis of a biological analysis of 8 flanking sequences in the DNA of B melitensis. The HOOF-Prints technique was used to identify wild-type and vaccine strains of B melitensis...
April 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28341394/serum-biochemical-profile-and-molecular-detection-of-pathogens-in-semen-of-infertile-male-dromedary-camels-camelus-dromedarius
#2
Khaled A Al-Busadah, Sabry M El-Bahr, Abdelmalik I Khalafalla
Detection of pathogens in the semen of camels has not been completely elucidated. Therefore, the current study aimed to determine the association of some economically important pathogens with infertility in 94 male infertile camels through molecular detection and estimation of selected biochemical parameters in serum of these animals compared with a control non infected fertile animals (n=40). PCR analysis of semen samples of infertile camels indicated that, four potential pathogens namely Mycoplasma spp., Leptospira spp...
March 9, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28340558/a-novel-real-time-pcr-assay-for-specific-detection-of-brucella-melitensis
#3
Rene Kaden, Sevinc Ferrari, Erik Alm, Tara Wahab
BACKGROUND: Brucellosis is a zoonosis that occurs worldwide. The disease has been completely eradicated in livestock in Sweden in 1994, and all cases of confirmed human brucellosis are imported into Sweden from other countries. However, due to an increase in the number of refugees and asylum seekers from the middle-east to Sweden, there is a need to improve the current diagnostic methodology for Brucella melitensis. Whilst culture of Brucella species can be used as a diagnostic tool, real-time PCR approaches provide a much faster result...
March 24, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28337351/a-rare-form-of-brucella-bursitis-with-negative-serology-a-case-report-and-literature-review
#4
F M Almajid
Brucellosis is still endemic in certain parts of the world including the Mediterranean, the Middle East, Latin America, and African regions. Osteoarticular manifestations are common presenting features. Brucellosis presenting as prepatellar bursitis has already been reported. We present a case of seronegative olecranon bursitis with positive blood and aspirate cultures. The patient improved remarkably by treatment with streptomycin and doxycycline with no evidence or relapse.
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/28334833/chip-seq-analysis-of-the-luxr-type-regulator-vjbr-reveals-novel-insights-into-the-brucella-virulence-gene-expression-network
#5
Claudia L Kleinman, Gabriela Sycz, Hernán R Bonomi, Romina M Rodríguez, Angeles Zorreguieta, Rodrigo Sieira
LuxR-type transcription factors control diverse physiological functions necessary for bacterial adaptation to environmental changes. In the intracellular pathogen Brucella, the LuxR homolog VjbR has been shown to regulate the expression of virulence factors acting at early stages of the intracellular infection and, directly or indirectly, hundreds of additional genes. However, the precise determination of VjbR direct targets has so far proved elusive. Here, we performed chromatin immunoprecipitation of VjbR followed by next-generation sequencing (ChIP-seq)...
March 11, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28325625/-serological-detection-of-brucella-suis-influenza-virus-and-aujeszky-s-disease-virus-in-backyard-and-small-swine-holders-in-argentina
#6
Marina Dibarbora, Javier A Cappuccio, María N Aznar, Fernando A Bessone, Hernán Piscitelli, Ariel J Pereda, Daniel R Pérez
Farmers raising less than 100 sows represent more than 99% of swine producers in Argentina, although little is known about their sanitary status and productive characteristics in the country. Sanitary and productive information was obtained. Furthermore, samples for serological studies were taken to detect antibodies against Brucella suis (Bs), Aujeszky's disease virus (AV) and influenza virus (IV) in 68 backyard and small producers with less than 100 sows located in the north, central and south regions of Argentina...
March 18, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28325071/a-rare-case-of-anterior-mediastinal-mass-caused-by-brucella-infection
#7
Feridoun Sabzi, Reza Faraji
A previously healthy man, who had undergone coronary artery bypass 10 years earlier and had been diagnosed with brucellosis due to Brucella septicemia after Brucella arthritis, presented with chest pain and high fever. Anti- Brucella antibiotics were started, but after 4 weeks, his high fever remained. An infected mass was confirmed by computed tomography, and surgical intervention was performed via a median sternotomy. A large amount of thick pus gushed from an abscess in the upper mediastinum. The abscess cavity had a thick granulation wall, and cultured pus was positive for Brucella only...
March 2017: Asian Cardiovascular & Thoracic Annals
https://www.readbyqxmd.com/read/28318383/detection-of-brucellosis-in-sika-deer-cervus-nippon-through-loop-mediated-isothermal-amplification-lamp
#8
Qianhong Liu, Jie Wei, Qingsong Sun, Ben Wang, Yuting Wang, Ying Hu, Wenrong Wu
Brucellosis (Brucella bovis) in sika deer ( Cervus nippon ) can cause enormous losses to stag breeding, especially in areas in which stag breeding has become an important industry. It also poses a threat to humans because it is a zoonotic disease. Use of the loop-mediated isothermal amplification (LAMP) assay has been poorly described in the diagnosis of brucellosis in deer. We developed a LAMP assay targeting the omp25 gene sequence to detect brucellosis (Brucella bovis) in sika deer. The reaction can be completed in 60 min at 63 C and, with a detection limit of 17 pg, it was more sensitive than conventional PCR, with its detection limit of 1...
March 20, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28317992/synthetic-glycoconjugates-characterize-the-fine-specificity-of-brucella-a-and-m-monoclonal-antibodies
#9
Satadru Sekhar Mandal, N Vijaya Ganesh, Joanna M Sadowska, David R Bundle
The dominant cell wall antigen of Brucella bacteria is the O-polysaccharide component of the smooth lipopolysaccharide. Infection by various Brucella biovars causes abortions and infertility in a wide range of domestic and wild animals and debilitating disease in humans. Diagnosis relies on the detection of antibodies to the A and M antigens expressed in the O-polysaccharide. This molecule is a homopolymer of the rare monosaccharide, 4-formamido-4,6-dideoxy-d-mannopyranose (Rha4NFo). The A epitope is created by a uniform α1,2 linked internal polymeric sequence capped by a distinct tetrasaccharide sequence defining the M antigen...
March 20, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28316045/nitrogen-removal-and-microbial-community-shift-in-an-aerobic-denitrification-reactor-bioaugmented-with-a-pseudomonas-strain-for-coal-based-ethylene-glycol-industry-wastewater-treatment
#10
Cong Du, Chong-Wei Cui, Shan Qiu, Sheng-Nan Shi, Ang Li, Fang Ma
An aerobic denitrification system, initially bioaugmented with Pseudomonas strain T13, was established to treat coal-based ethylene glycol industry wastewater, which contained 3219 ± 86 mg/L total nitrogen (TN) and 1978 ± 14 mg/L NO3(-)-N. In the current study, a stable denitrification efficiency of 53.7 ± 4.7% and nitrite removal efficiency of 40.1 ± 2.7% were achieved at different diluted influent concentrations. Toxicity evaluation showed that a lower toxicity of effluent was achieved when industry wastewater was treated by stuffing biofilm communities compared to suspended communities...
March 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28315659/lessons-learned-with-molecular-methods-targeting-the-bcsp-31-membrane-protein-for-diagnosis-of-human-brucellosis
#11
REVIEW
Rocio Sanjuan-Jimenez, Juan D Colmenero, Pilar Morata
Brucellosis remains an emerging and re-emerging zoonosis worldwide causing high human morbidity. It usually affects persons who are permanently exposed to fastidious microorganisms of the Brucella genus and has a nonspecific clinical picture. Thus, diagnosis of brucellosis can sometimes be difficult. Molecular techniques have recently been found very useful in the diagnosis of brucellosis together with its common and very diverse focal complications. We herein review all the lessons learned by our group concerning the molecular diagnosis of human brucellosis over the last twenty years...
March 16, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28300645/boar-hunting-and-brucellosis-caused-by-brucella-suis
#12
REVIEW
Carlos Franco-Paredes, Daniel Chastain, Peter Taylor, Stephen Stocking, Brenda Sellers
Brucellosis remains as a significant public health concern in many areas where the infection persists in domestic hosts (i.e. goats, cattle, and domestic swine) with subsequent risk of transmission to human populations. Brucellosis caused by B. suis remains an important threat to human populations in many countries exposed to domestic and feral swine. In the U.S., swine brucellosis has been under control for many years. Meanwhile, it is a widespread infection among feral swine, particularly in the Southeastern United States; and exposure to infected animals pose a growing threat to humans...
March 12, 2017: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/28300153/brucella-spp-of-amphibians-comprise-genomically-diverse-motile-strains-competent-for-replication-in-macrophages-and-survival-in-mammalian-hosts
#13
Sascha Al Dahouk, Stephan Köhler, Alessandra Occhialini, María Pilar Jiménez de Bagüés, Jens Andre Hammerl, Tobias Eisenberg, Gilles Vergnaud, Axel Cloeckaert, Michel S Zygmunt, Adrian M Whatmore, Falk Melzer, Kevin P Drees, Jeffrey T Foster, Alice R Wattam, Holger C Scholz
Twenty-one small Gram-negative motile coccobacilli were isolated from 15 systemically diseased African bullfrogs (Pyxicephalus edulis), and were initially identified as Ochrobactrum anthropi by standard microbiological identification systems. Phylogenetic reconstructions using combined molecular analyses and comparative whole genome analysis of the most diverse of the bullfrog strains verified affiliation with the genus Brucella and placed the isolates in a cluster containing B. inopinata and the other non-classical Brucella species but also revealed significant genetic differences within the group...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28296882/detection-and-characterization-of-brucella-spp-in-bovine-milk-in-small-scale-urban-and-peri-urban-farming-in-tajikistan
#14
Elisabeth Lindahl-Rajala, Tove Hoffman, David Fretin, Jacques Godfroid, Nosirjon Sattorov, Sofia Boqvist, Åke Lundkvist, Ulf Magnusson
Brucellosis is one of the most common zoonoses globally, and Central Asia remains a Brucella hotspot. The World Health Organization classifies brucellosis as a neglected zoonotic disease that is rarely in the spotlight for research and mainly affects poor, marginalized people. Urban and peri-urban farming is a common practice in many low-income countries, and it increases the incomes of families that are often restrained by limited economic resources. However, there is a concern that the growing number of people and livestock living close together in these areas will increase the transmission of zoonotic pathogens such as Brucella...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28296215/brucella-canis-infection-in-dogs-from-commercial-breeding-kennels-in-brazil
#15
L B Keid, D P Chiebao, M C A Batinga, T Faita, J A Diniz, T M F de S Oliveira, H L Ferreira, R M Soares
Canine brucellosis caused by Brucella canis is a neglected zoonosis worldwide and is a leading cause of reproductive failure in dogs, often causing substantial economic losses in breeding kennels. This study aimed to investigate the occurrence of B. canis infection in dogs of commercial breeding kennels located in São Paulo State, Brazil. A total of 753 dogs (183 males and 570 females) from 38 commercial kennels were clinically examined, and blood samples were collected for brucellosis diagnosis through blood culture...
March 13, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28284850/molecular-prevalence-of-putative-virulence-associated-genes-in-brucella-melitensis-and-brucella-abortus-isolates-from-human-and-livestock-specimens-in-iran
#16
Iman Hashemifar, Abbas Yadegar, Faramarz Masjedian Jazi, Nour Amirmozafari
Molecular prevalence of nine putative virulence factors in two more prevalent Brucella species in Iranian patients and livestock was investigated. During five years (2010-2015), 120 human and animal specimens were collected from three geographical areas of Iran. All samples were cultured in blood culture media and subcultured into Brucella agar medium. Nine primer pairs were designed for detection of VirB2, VirB5, VceC, BtpA, BtpB, PrpA, BetB, BPE275 and BSPB virulence factors using PCR and sequence analysis...
March 8, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28283872/screening-food-borne-and-zoonotic-pathogens-associated-with-livestock-practices-in-the-sumapaz-region-cundinamarca-colombia
#17
Nelson E Arenas, Diego A Abril, Paola Valencia, Surabhi Khandige, Carlos Yesid Soto, Vilma Moreno-Melo
Hazardous practices regarding antibiotics misuse, unsanitary milking procedures, and the commercial sales of raw milk and unpasteurized dairy products are currently being practiced by livestock farmers in the Sumapaz region (Colombia). The purpose of this study was to screen for food-borne and zoonotic pathogens associated with local livestock practices. We evaluated 1098 cows from 46 livestock farms in the Sumapaz region that were selected by random. Of the total population of cattle, 962 animals (88%) were tested for bovine TB using a caudal-fold tuberculin test and 546 (50%) for brucellosis by a competitive ELISA...
March 11, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28282974/a-rapid-detection-method-of-brucella-with-quantum-dots-and-magnetic-beads-conjugated-with-different-polyclonal-antibodies
#18
Dandan Song, Xiaofeng Qu, Yushen Liu, Li Li, Dehui Yin, Juan Li, Kun Xu, Renguo Xie, Yue Zhai, Huiwen Zhang, Hao Bao, Chao Zhao, Juan Wang, Xiuling Song, Wenzhi Song
Brucella spp. are facultative intracellular bacteria that cause zoonotic disease of brucellosis worldwide. Traditional methods for detection of Brucella spp. take 48-72 h that does not meet the need of rapid detection. Herein, a new rapid detection method of Brucella was developed based on polyclonal antibody-conjugating quantum dots and antibody-modified magnetic beads. First, polyclonal antibodies IgG and IgY were prepared and then the antibody conjugated with quantum dots (QDs) and immunomagnetic beads (IMB), respectively, which were activated by N-(3-dimethylaminopropyl)-N'-ethylcar-bodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to form probes...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28281771/an-evaluation-of-serological-tests-in-the-diagnosis-of-bovine-brucellosis-in-naturally-infected-cattle-in-kwazulu-natal-province-in-south-africa
#19
Songelwayo L Chisi, Yoanda Marageni, Prebashni Naidoo, Gloria Zulu, George W Akol, Henriette Van Heerden
The diagnostic sensitivity (DSe) of the Rose Bengal test (RBT), the complement fixation test (CFT), the serum agglutination test (SAT), the competitive enzyme-linked immunosorbent assay (cELISA) and the indirect ELISA (iELISA) were determined in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture (gold standard). Natural brucellosis infection status of animals was determined by culturing and identification of Brucella abortus biovar 1 from abomasal fluid, milk, hygroma fluid, lymph nodes or uterine discharges samples...
February 28, 2017: Journal of the South African Veterinary Association
https://www.readbyqxmd.com/read/28274160/brucella-suis-carbonic-anhydrases-and-their-inhibitors-towards-alternative-antibiotics
#20
REVIEW
Stephan Köhler, Safia Ouahrani-Bettache, Jean-Yves Winum
Carbonic anhydrases have started to emerge as new potential antibacterial targets for several pathogens. Two β-carbonic anhydrases, denominated bsCA I and bsCA II, have been isolated and characterized from the bacterial pathogen Brucella suis, the causative agent of brucellosis or Malta fever. These enzymes have been investigated in detail and a wide range of classical aromatic and heteroaromatic sulfonamides as well as carbohydrate-based compounds have been found to inhibit selectively and efficiently Brucella suis carbonic anhydrases...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
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