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Shaohua Wu, Bin Duan, Xiaohong Qin, Jonathan T Butcher
Regeneration and repair of injured or diseased heart valves remains a clinical challenge. Tissue engineering provides a promising treatment approach to facilitate living heart valve repair and regeneration. Three-dimensional (3D) biomimetic scaffolds that possess heterogeneous and anisotropic features that approximate those of native heart valve tissue are beneficial to the successful in vitro development of tissue engineered heart valves (TEHV). Here we report the development and characterization of a novel composite scaffold consisting of nano- and micro-scale fibrous woven fabrics and 3D hydrogels by using textile techniques combined with bioactive hydrogel formation...
January 18, 2017: Acta Biomaterialia
Sharan Ramaswamy, Makensley Lordeus, Omkar V Mankame, Lilliam Valdes-Cruz, Steven Bibevski, Sarah M Bell, Ivan Baez, Frank Scholl
Infants and children born with severe cardiac valve lesions have no effective long term treatment options since currently available tissue or mechanical prosthetic valves have sizing limitations and no avenue to accommodate the growth of the pediatric patient. Tissue engineered heart valves (TEHVs) which could provide for growth, self-repair, infection resistance, and long-term replacement could be an ideal solution. Porcine small intestinal submucosa (PSIS) has recently emerged as a potentially attractive bioscaffold for TEHVs...
December 19, 2016: Cardiovascular Engineering and Technology
Tao Jin, Ilinca Stanciulescu
Poly(ethylene glycol) diacrylate (PEGDA) hydrogels can be potentially used as scaffold material for tissue engineered heart valves (TEHVs) due to their good biocompatibility and biomechanical tunability. The photolithographic patterning technique is an effective approach to pattern PEGDA hydrogels to mimic the mechanical behavior of native biological tissues that are intrinsically anisotropic. The material properties of patterned PEGDA hydrogels largely depend on the pattern topology. In this paper, we adopt a newly proposed computational framework for fibrous biomaterials to numerically investigate the influence of pattern topology, including pattern ratio, orientation and waviness, on the mechanical behavior of patterned PEGDA hydrogels...
November 14, 2016: Acta Biomaterialia
Simone A Huygens, Maureen P M H Rutten-van Mölken, Jos A Bekkers, Ad J J C Bogers, Carlijn V C Bouten, Steven A J Chamuleau, Peter P T de Jaegere, Arie Pieter Kappetein, Jolanda Kluin, Nicolas M D A van Mieghem, Michel I M Versteegh, Maarten Witsenburg, Johanna J M Takkenberg
OBJECTIVE: The future promises many technological advances in the field of heart valve interventions, like tissue-engineered heart valves (TEHV). Prior to introduction in clinical practice, it is essential to perform early health technology assessment. We aim to develop a conceptual model (CM) that can be used to investigate the performance and costs requirements for TEHV to become cost-effective. METHODS: After scoping the decision problem, a workgroup developed the draft CM based on clinical guidelines...
2016: Open Heart
Toshiharu Shinoka, Hideki Miyachi
The development of surgically implantable heart valve prostheses has contributed to improved outcomes in patients with cardiovascular disease. However, there are drawbacks, such as risk of infection and lack of growth potential. Tissue-engineered heart valve (TEHV) holds great promise to address these drawbacks as the ideal TEHV is easily implanted, biocompatible, non-thrombogenic, durable, degradable, and ultimately remodels into native-like tissue. In general, three main components used in creating a tissue-engineered construct are (1) a scaffold material, (2) a cell type for seeding the scaffold, and (3) a subsequent remodeling process driven by cell accumulation and proliferation, and/or biochemical and mechanical signaling...
November 2016: World Journal for Pediatric & Congenital Heart Surgery
Mehrdad Namdari, Ali Eatemadi
Heart valves are currently under thorough investigation in tissue engineering (TE) research. Mechanical and biological heart valve prostheses which are recently used have several shortcomings. While allogenic and xenogenic biological prostheses are related to graft rejection, degeneration and thrombosis, resulting in a high rate of reoperation. Mechanical prostheses on the other hand are based on metallic, carbon, and polymeric components, and require continuous treatment with anticoagulant, which result in adverse reactions, e...
December 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Anwarul Hasan, John Saliba, Hassan Pezeshgi Modarres, Ahmed Bakhaty, Amir Nasajpour, Mohammad R K Mofrad, Amir Sanati-Nezhad
Due to the increased morbidity and mortality resulting from heart valve diseases, there is a growing demand for off-the-shelf implantable tissue engineered heart valves (TEHVs). Despite the significant progress in recent years in improving the design and performance of TEHV constructs, viable and functional human implantable TEHV constructs have remained elusive. The recent advances in micro and nanoscale technologies including the microfabrication, nano-microfiber based scaffolds preparation, 3D cell encapsulated hydrogels preparation, microfluidic, micro-bioreactors, nano-microscale biosensors as well as the computational methods and models for simulation of biological tissues have increased the potential for realizing viable, functional and implantable TEHV constructs...
October 2016: Biomaterials
Yang Yang, Chongxi Fan, Chao Deng, Lin Zhao, Wei Hu, Shouyin Di, Zhiqiang Ma, Yu Zhang, Zhigang Qin, Zhenxiao Jin, Xiaolong Yan, Shuai Jiang, Yang Sun, Wei Yi
Tissue-engineered heart valves (TEHVs) are a promising treatment for valvular heart disease, although their application is limited by high flow shear stress (FSS). Melatonin has a wide range of physiological functions and is currently under clinical investigation for expanded applications; moreover, extensive protective effects on the cardiovascular system have been reported. In this study, we investigated the protection conferred by melatonin supplementation against FSS-induced injury in bone marrow mesenchymal stem cells (BMSCs) and elucidated the potential mechanism in this process...
March 2016: Journal of Pineal Research
Sandra Loerakker, Tommaso Ristori, Frank P T Baaijens
One of the most critical problems in heart valve tissue engineering is the progressive development of valvular insufficiency due to leaflet retraction. Understanding the underlying mechanisms of this process is crucial for developing tissue-engineered heart valves (TEHVs) that maintain their functionality in the long term. In the present study, we adopted a computational approach to predict the remodeling process in TEHVs subjected to dynamic pulmonary and aortic pressure conditions, and to assess the risk of valvular insufficiency...
May 2016: Journal of the Mechanical Behavior of Biomedical Materials
Sasmita Rath, Manuel Salinas, Ana G Villegas, Sharan Ramaswamy
For treatment of critical heart valve diseases, prosthetic valves perform fairly well in most adults; however, for pediatric patients, there is the added requirement that the replacement valve grows with the child, thus extremely limiting current treatment options. Tissue engineered heart valves (TEHV), such as those derived from autologous bone marrow stem cells (BMSCs), have the potential to recapitulate native valve architecture and accommodate somatic growth. However, a fundamental pre-cursor in promoting directed integration with native tissues rather than random, uncontrolled growth requires an understanding of BMSC mechanobiological responses to valve-relevant mechanical environments...
2015: PloS One
Gabriel L Converse, Eric E Buse, Kari R Neill, Christopher R McFall, Holley N Lewis, Mitchell C VeDepo, Rachael W Quinn, Richard A Hopkins
Heart valve tissue engineering offers the promise of improved treatments for congenital heart disorders; however, widespread clinical availability of a tissue engineered heart valve (TEHV) has been hindered by scientific and regulatory concerns, including the lack of a disposable, bioreactor system for nondestructive valve seeding and mechanical conditioning. Here we report the design for manufacture and the production of full scale, functional prototypes of such a system. To evaluate the efficacy of this bioreactor as a tool for seeding, ovine aortic valves were decellularized and subjected to seeding with human mesenchymal stem cells (hMSC)...
February 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Maryam Eslami, Gholamreza Javadi, Nasser Agdami, Mohammad Ali Shokrgozar
OBJECTIVE: The incidence of heart valve disease is increasing worldwide and the number of heart valve replacements is expected to increase in the future. By mimicking the main tissue structures and properties of heart valve, tissue engineering offers new options for the replacements. Applying an appropriate scaffold in fabricating tissue-engineered heart valves (TEHVs) is of importance since it affects the secretion of the main extracellular matrix (ECM) components, collagen 1 and elastin, which are crucial in providing the proper mechanical properties of TEHVs...
2015: Cell Journal
Francesco C Origgi, Marco Tecilla, Paola Pilo, Fabio Aloisio, Patricia Otten, Lisandra Aguilar-Bultet, Ursula Sattler, Paola Roccabianca, Carlos H Romero, David C Bloom, Elliott R Jacobson
We report the first de novo sequence assembly and analysis of the genome of Testudinid herpesvirus 3 (TeHV3), one of the most pathogenic chelonian herpesviruses. The genome of TeHV3 is at least 150,080 nucleotides long, is arranged in a type D configuration and comprises at least 102 open reading frames extensively co-linear with those of Human herpesvirus 1. Consistently, the phylogenetic analysis positions TeHV3 among the Alphaherpesvirinae, closely associated with Chelonid herpesvirus 5, a Scutavirus. To date, there has been limited genetic characterization of TeHVs and a resolution beyond the genotype was not feasible because of the lack of informative DNA sequences...
2015: PloS One
Isra Marei, Adrian Chester, Ivan Carubelli, Themistoklis Prodromakis, Tatiana Trantidou, Magdi H Yacoub
BACKGROUND: Scaffolds are a key component of tissue-engineered heart valves (TEHVs). Several approaches had been adopted in the design of scaffolds using both natural and synthetic resources. We have investigated the suitability of parylene C (PC), a vapor deposited polymeric material, for the use as a scaffold in TEHV. AIMS: To evaluate the adsorption of extracellular matrix components onto plasma-activated PC and study the biocompatibility of PC by measuring cellular adhesion, viability, apoptosis, and phenotypic expression of valve endothelial and interstitial cells...
October 2015: Tissue Engineering. Part A
Samaneh Ghazanfari, Anita Driessen-Mol, Bart Sanders, Petra E Dijkman, Simon P Hoerstrup, Frank P T Baaijens, Carlijn V C Bouten
BACKGROUND: Decellularized tissue-engineered heart valves (TEHVs) are under investigation as alternative for current heart valve prostheses with the potential to rapidly repopulate with cells within the body. Ideally, these valves are stented for transapical or minimally invasive delivery. It is unclear if and how the matrix of these valves remodels under in vivo hemodynamic loading conditions and in the presence of a stent. Here, we study the evolution of collagen orientation and tissue maturation in the wall of stented decellularized TEHVs with time after implantation...
August 2015: Tissue Engineering. Part A
Sasmita Rath, Manuel Salinas, Smita Bhatacharjee, Sharan Ramaswamy
Fluid-induced oscillatory shear stress (OSS) and nicotine are known antagonists in cardiovascular disease. However, from a regenerative medicine standpoint, we hypothesized that these parameters may support the cell differentiation of bone marrow mesenchymal stem cells (BMMSCs) for engineering heart valves. In this study, OSS and nicotine (10-6M) were applied individually to BMMSCs in monolayer culture. In both cases, a significantly higher expression of CD31 was detected compared to corresponding controls (p<0...
2015: Journal of Long-term Effects of Medical Implants
Iyore A James, Tai Yi, Shuhei Tara, Cameron A Best, Alexander J Stuber, Kejal V Shah, Blair F Austin, Tadahisa Sugiura, Yong-Ung Lee, Joy Lincoln, Aaron J Trask, Toshiharu Shinoka, Christopher K Breuer
Decellularized allograft heart valves have been used as tissue-engineered heart valve (TEHV) scaffolds with promising results; however, little is known about the cellular mechanisms underlying TEHV neotissue formation. To better understand this phenomenon, we developed a murine model of decellularized pulmonary heart valve transplantation using a hemodynamically unloaded heart transplant model. Furthermore, because the hemodynamics of blood flow through a heart valve may influence morphology and subsequent function, we describe a modified loaded heterotopic heart transplant model that led to an increase in blood flow through the pulmonary valve...
September 2015: Tissue Engineering. Part C, Methods
Miriam Weber, Israel Gonzalez de Torre, Ricardo Moreira, Julia Frese, Caroline Oedekoven, Matilde Alonso, Carlos J Rodriguez Cabello, Stefan Jockenhoevel, Petra Mela
Heart valves are elaborate and highly heterogeneous structures of the circulatory system. Despite the well accepted relationship between the structural and mechanical anisotropy and the optimal function of the valves, most approaches to create tissue-engineered heart valves (TEHVs) do not try to mimic this complexity and rely on one homogenous combination of cells and materials for the whole construct. The aim of this study was to establish an easy and versatile method to introduce spatial diversity into a heart valve fibrin scaffold...
August 2015: Tissue Engineering. Part C, Methods
Bin Duan, Laura A Hockaday, Shoshana Das, Charlie Xu, Jonathan T Butcher
Living tissue-engineered heart valves (TEHV) would be a major benefit for children who require a replacement with the capacity for growth and biological integration. A persistent challenge for TEHV is accessible human cell source(s) that can mimic native valve cell phenotypes and matrix remodeling characteristics that are essential for long-term function. Mesenchymal stem cells derived from bone marrow (BMMSC) or adipose tissue (ADMSC) are intriguing cell sources for TEHV, but they have not been compared with pediatric human aortic valve interstitial cells (pHAVIC) in relevant 3D environments...
August 2015: Tissue Engineering. Part C, Methods
Nafiseh Masoumi, Nasim Annabi, Alexander Assmann, Benjamin L Larson, Jesper Hjortnaes, Neslihan Alemdar, Mahshid Kharaziha, Keefe B Manning, John E Mayer, Ali Khademhosseini
Tissue engineered heart valves (TEHVs) that can grow and remodel have the potential to serve as permanent replacements of the current non-viable prosthetic valves particularly for pediatric patients. A major challenge in designing functional TEHVs is to mimic both structural and anisotropic mechanical characteristics of the native valve leaflets. To establish a more biomimetic model of TEHV, we fabricated tri-layered scaffolds by combining electrospinning and microfabrication techniques. These constructs were fabricated by assembling microfabricated poly(glycerol sebacate) (PGS) and fibrous PGS/poly(caprolactone) (PCL) electrospun sheets to develop elastic scaffolds with tunable anisotropic mechanical properties similar to the mechanical characteristics of the native heart valves...
September 2014: Biomaterials
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