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c-kit chemokine

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https://www.readbyqxmd.com/read/30066869/extracellular-matrix-promotes-proliferation-migration-and-adhesion-of-airway-smooth-muscle-cells-in-a-rat-model-of-chronic-obstructive-pulmonary-disease-via-upregulation-of-the-pi3k-akt-signaling-pathway
#1
Zhengyan Wang, Rui Li, Rui Zhong
Extracellular matrix (ECM) creates the tissue microenvironment and serves a role in airway wall remodeling in chronic obstructive pulmonary disease (COPD). However, the biological function of ECM in COPD remains to be elucidated. In the present study, 24 healthy Sprague Dawley rats were randomized to normal and COPD groups. COPD was established by intratracheal injection with lipopolysaccharide over 30 days. Subsequently, airway smooth muscle cells (ASMCs) were isolated from rats and served as a model to assess the effects of three ECM components, including collagen type I, laminin and collagen type III (COL‑3)...
July 25, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/30015967/bioinformatics-approach-reveals-the-key-role-of-c%C3%A2-x%C3%A2-c-motif-chemokine-receptor-2-in-endometriosis-development
#2
Aili Tan, Ruoyu Luo, Hua Liang, Mengru Li, Peng Ruan
Endometriosis is a common gynecological disease, affecting 6‑10% of women of reproductive age. The precise mechanisms underlying the development of endometriosis remain unclear. In the present study, a bioinformatics approach was applied to systematically identify the pathways and genes involved in the development of endometriosis and to discover potential biomarkers. The gene expression profiles of GSE6364, a microarray dataset of endometrial biopsies obtained from women with or without endometriosis, was downloaded from the Gene Expression Omnibus DataSets database that stores original submitter‑supplied records (series, samples and platforms), as well as curated datasets...
September 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29940819/investigation-of-plasma-inflammatory-profile-in-diabetic-patients-with-abdominal-aortic-aneurysm-a-pilot-study
#3
Fabien Lareyre, Claudine Moratal, Julien Chikande, Elixène Jean-Baptiste, Réda Hassen-Khodja, Jaap Neels, Giulia Chinetti, Juliette Raffort
INTRODUCTION: Clinical studies have unraveled a negative association between diabetes and abdominal aortic aneurysm (AAA), but the mechanisms involved are still poorly understood. The aim of this study was to determine whether diabetic patients with AAA had a distinct plasma inflammatory profile compared to nondiabetic patients. METHODS: Plasma samples were obtained from 10 diabetic patients with AAA and 10 nondiabetic patients with AAA. The relative protein expression of 92 inflammatory-related human protein biomarkers was assessed by proximity extension assay technology using Proseek Multiplex Inflammation I kit (Olink)...
January 1, 2018: Vascular and Endovascular Surgery
https://www.readbyqxmd.com/read/29928346/overexpression-of-hsa-mir-125a-5p-enhances-proliferation-migration-and-invasion-of-head-and-neck-squamous-cell-carcinoma-cell-lines-by-upregulating-c-c-chemokine-receptor-type-7
#4
Shan Jin, Min-Da Liu, Hong Wu, Pai Pang, Song Wang, Zhen-Ning Li, Chang-Fu Sun, Fa-Yu Liu
Head and neck squamous cell carcinoma (HNSCC) is usually diagnosed accompanied by lymph node metastasis. C-C chemokine receptor type 7 (CCR7) is associated with the invasion and metastasis of tumors in HNSCC through various signaling pathways. The role of hsa-miR-125a-5p in HNSCC remains unclear. The present study was performed to investigate the association between hsa-miR-125a-5p and CCR7 in HNSCC. Reverse transcription-quantitative polymerase chain reaction was applied to analyze the expression of hsa-miR-125a-5p in clinical samples...
June 2018: Oncology Letters
https://www.readbyqxmd.com/read/29845205/effect-of-co%C3%A2-culture-with-amniotic-epithelial-cells-on-the-biological-characteristics-of-amniotic-mesenchymal-stem-cells
#5
Li-Jing Ran, Yun Zeng, Shao-Chun Wang, Di-Si Zhang, Min Hong, Shao-You Li, Jian Dong, Ming-Xia Shi
The aim of the present study was to investigate the effect of co‑culture with amniotic epithelial cells (AECs) on the biological characteristics of amniotic mesenchymal stem cells (AMSCs), to compare the expression of C‑X‑C motif chemokine receptor 4 (CXCR4) in co‑cultured AMSCs and to investigate the roles of the stromal cell‑derived factor‑1 (SDF‑1)/CXCR4 axis in the homing and migration of AMSCs. AMSCs were isolated from human amniotic membranes, purified and then differentiated into osteoblasts and adipocytes in vitro, which was verified by von Kossa Staining and Oil Red O staining...
July 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29805477/high-expression-of-cxcr4-and-stem-cell-markers-in-a-monocrotaline-and-chronic-hypoxia-induced-rat-model-of-pulmonary-arterial-hypertension
#6
Tingting Zhang, Nanako Kawaguchi, Emiko Hayama, Yoshiyuki Furutani, Toshio Nakanishi
Pulmonary arterial hypertension (PAH) is a severe and fatal clinical syndrome. C-X-C chemokine receptor type 4 (CXCR4) is known to serve a key role in recruiting mesenchymal stem cells (MSCs) from the bone marrow. In the present study, a rat model of PAH induced by 5 weeks of chronic hypoxia and treatment with a single injection of monocrotaline (60 mg/kg) was used to investigate the involvement of CXCR4 in PAH. Successful establishment of the PAH model was confirmed by significant differences between the PAH and control groups in right ventricular systolic pressure, Fulton index, wall thickness, vascular occlusion score determined by immunohistochemical staining and the expression of inflammatory markers measured by reverse transcription-quantitative polymerase chain reaction (RT-qPCR)...
June 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29796010/cxcr-4-expression-by-circulating-endothelial-progenitor-cells-and-sdf-1-serum-levels-are-elevated-in-septic-patients
#7
Christian Patry, Daniela Stamm, Christian Betzen, Burkhard Tönshoff, Benito A Yard, Grietje Ch Beck, Neysan Rafat
Background: Endothelial progenitor cell (EPC) numbers are increased in septic patients and correlate with survival. In this study, we investigated, whether surface expression of chemokine receptors and other receptors important for EPC homing is upregulated by EPC from septic patients and if this is associated with clinical outcome. Methods: Peripheral blood mononuclear cells from septic patients ( n  = 30), ICU control patients ( n  = 11) and healthy volunteers ( n  = 15) were isolated by Ficoll density gradient centrifugation...
2018: Journal of Inflammation
https://www.readbyqxmd.com/read/29787588/angiogenic-and-pleiotropic-effects-of-vegf165-and-hgf-combined-gene-therapy-in-a-rat-model-of-myocardial-infarction
#8
Pavel I Makarevich, Konstantin V Dergilev, Zoya I Tsokolaeva, Maria A Boldyreva, Evgeniy K Shevchenko, Evgeny V Gluhanyuk, Julia O Gallinger, Mikhail Yu Menshikov, Yelena V Parfyonova
Since development of plasmid gene therapy for therapeutic angiogenesis by J. Isner this approach was an attractive option for ischemic diseases affecting large cohorts of patients. However, first placebo-controlled clinical trials showed its limited efficacy questioning further advance to practice. Thus, combined methods using delivery of several angiogenic factors got into spotlight as a way to improve outcomes. This study provides experimental proof of concept for a combined approach using simultaneous delivery of VEGF165 and HGF genes to alleviate consequences of myocardial infarction (MI)...
2018: PloS One
https://www.readbyqxmd.com/read/29762844/free-fatty-acids-mediates-human-umbilical-vein-endothelial-cells-inflammation-through-toll-like-receptor-4
#9
L Chen, C-X Yu, B Song, W Cai, C Liu, Q-B Guan
OBJECTIVE: To investigate the role of Toll-like receptor-4 (TLR4) in the free fatty acids (FFAs) induced human umbilical vein endothelial cells (HUVECs) inflammation and to explore the underlying mechanisms. MATERIALS AND METHODS: HUVECs and HEK293 cell lines were obtained from Shanghai Type Culture Collection. Cell counting kit-8 (CCK8) and flow cytometry (FCM) were performed to examine the cell viability and apoptosis rate of HUVECs induced by FFAs treatments with or without infection of toll-like receptor-4 interference (TLR4i) adenovirus...
April 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29731866/microrna-hsa-let-7e-5p-as-a-potential-prognosis-marker-for-rectal-carcinoma-with-liver-metastases
#10
Wenfeng Chen, Guosheng Lin, Yizhou Yao, Jishen Chen, Hanli Shui, Qinghai Yang, Xiaoya Wang, Xiaoyuan Weng, Ling Sun, Fei Chen, Sheng Yang, Yufeng Yang, Yongjian Zhou
MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that target mRNAs for translational repression or cleavage. The present study was conducted to identify differentially expressed miRNAs in primary tumor tissues of rectal carcinoma (RC) that may be associated with heterochrony hepatic metastasis (HHM). Samples were collected exclusively from patients with RC but not colon cancer (CC); Next-generation high-throughput sequencing technology and bioinformatics tools were used to profile and analyze small RNAs and their corresponding targets in primary tumor tissues with HHM (n=2) or without metastases (non-metastatic, NM; n=2)...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29693177/the-therapeutic-effect-of-artesunate-on-rosacea-through-the-inhibition-of-the-jak-stat-signaling-pathway
#11
Ting Li, Qingwen Zeng, Xingming Chen, Guojiang Wang, Haiqing Zhang, Aihua Yu, Hairui Wang, Yang Hu
Acne rosacea is a type of chronic dermatosis with the characteristics of erubescence, angiotelectasis and pustule formation. However, current treatment methods are limited due to the side effects. Artesunate demonstrated a promising therapeutic efficacy with a high safety margin. HaCaT cells were treated with antibacterial peptide LL‑37 to simulate rosacea caused by Demodex folliculorum (D. folliculorum) infection. Cell Counting kit 8 and flow cytometry assays were performed to measure cellular proliferation, apoptosis, the stage of the cell cycle and reactive oxygen species generation in order to determine the level of cell damage...
June 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29662241/low-mother-to-child-ccl22-chemokine-levels-are-inversely-related-to-mite-sensitization-and-asthma-in-early-childhood
#12
Chih-Yung Chiu, Kuan-Wen Su, Ming-Han Tsai, Man-Chin Hua, Sui-Ling Liao, Shen-Hao Lai, Li-Chen Chen, Tsung-Chieh Yao, Kuo-Wei Yeh, Jing-Long Huang
Few studies have addressed the mother-to-child transmission of Th2 immunity and the impact on the development of atopic diseases in early childhood. We investigated 186 children who were followed-up regularly for 4 years in a birth cohort study. The levels of Th2 related chemokine (C-C motif) ligand 17 (CCL17) and CCL22 were quantified in cord blood and at 1.5 years-of-age using multiplex Luminex kits. The levels of 125 pairs of CCL17 and CCL22 chemokines from birth to 1.5 years were recorded in this study...
April 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29520864/complex-association-patterns-for-inflammatory-mediators-in-induced-sputum-from-subjects-with-asthma
#13
A T Hastie, C Steele, C W Dunaway, W C Moore, B M Rector, E Ampleford, H Li, L C Denlinger, N Jarjour, D A Meyers, E R Bleecker
BACKGROUND: The release of various inflammatory mediators into the bronchial lumen is thought to reflect both the type and degree of airway inflammation, eosinophilic Th2, and Th9, or neutrophilic Th1, and Th17, in patients with asthma. AIMS: We investigated whether cytokines and chemokines differed in sputum from subjects with more severe compared with milder asthma and whether unbiased factor analysis of cytokine and chemokine groupings indicates specific inflammatory pathways...
July 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29520695/akip1-promotes-angiogenesis-and-tumor-growth-by-upregulating-cxc-chemokines-in-cervical-cancer-cells
#14
Wenying Zhang, Qiongwei Wu, Chao Wang, Longtao Yang, Ping Liu, Chengbin Ma
Upregulation of A-kinase-interacting protein 1 (AKIP1) has been observed in breast and esophageal cancers, indicating that AKIP1 may be a potent oncogenic protein. However, the role of AKIP1 in cervical cancer still remains unknown. This study aimed to explore the role of AKIP1 in cervical cancer and to investigate the underlying mechanism of AKIP1 in tumor growth. Expression of AKIP1 in cervical cancer cells was determined by qRT-PCR and western blotting. Cell-Light EdU and colony formation assays were used to determine cell proliferation...
March 8, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29434741/inhibition-of-c-x-c-motif-chemokine-10-reduces-graft-loss-mediated-by-memory-cd8-t-cells-in-a-rat-cardiac-re-transplant-model
#15
Jiacheng Xu, Teng Ma, Guorong Deng, Jiawei Zhuang, Cheng Li, Shaohu Wang, Chen Dai, Xiaobiao Zhou, Zhonggui Shan, Zhongquan Qi
The interaction of chemokine (C-X-C motif) ligand 10 (CXCL10) with its receptor (CXCR3) is a critical process in recruiting donor reactive T cells to a graft and alloantigen-specific memory T (Tm) cells exert a principal function in promoting graft dysfunction during accelerated cardiac rejection. However, whether CXCL10 chemokine exerts any effects on acute accelerated rejection mediated by CD8+ Tm cells in a re-transplant model has remained elusive. The present study established a cardiac transplant model by advanced microsurgery technology and improved organ storage...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29432913/effects-of-fluconazole-on-the-clinical-outcome-and-immune-response-in-fungal-co-infected-tuberculosis-patients
#16
Xiaojuan Ren, Wei Liu, Yi Liu
With overuse of the broad-spectrum antibiotics, the pulmonary fungal infection increasingly becomes the most common complication associated with senile pulmonary tuberculosis (TB) and attracts intensive attentions from clinicians. Here we presented the retrospective analysis of impact of fluconazole treatment on the clinical outcome and immune response in fungal co-infected tuberculosis patients. A randomized, double-blind, placebo-controlled trial of fluconazole (100 mg per day for consecutive weeks) in fungal-positive senile tuberculosis patients was conducted in our hospital...
April 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29422964/association-of-mast-cell-infiltration-with-gastric-cancer-progression
#17
Bei Zhong, Yu Li, Xiaodong Liu, Dongsheng Wang
The present study aimed to determine the expression of mast cells, C-C motif chemokine ligand 2 (CCL-2) and C-C motif chemokine receptor 2 (CCR2) in gastric cancer tumor tissue; and the association of mast cells with the proliferation, migration, invasion and apoptosis of gastric cancer cells. In addition, whether the stem cell factor (SCF)/c-Kit pathway was associated with the secretion of CCL-2 by gastric cancer cells was explored. Flow cytometry analysis and immunohistochemistry were used to observe the relative number of mast cells, and reverse transcription-quantitative polymerase chain reaction and western blot analysis were utilized to determine the expression of CCL-2 and CCR2 mRNA and protein...
January 2018: Oncology Letters
https://www.readbyqxmd.com/read/29348441/sdf1-gradient-associates-with-the-distribution-of-c-kit-cardiac-cells-in-the-heart
#18
Outi Renko, Anna-Maria Tolonen, Jaana Rysä, Johanna Magga, Erja Mustonen, Heikki Ruskoaho, Raisa Serpi
Identification of the adult cardiac stem cells (CSCs) has offered new therapeutic possibilities for treating ischemic myocardium. CSCs positive for the cell surface antigen c-Kit are known as the primary source for cardiac regeneration. Accumulating evidence shows that chemokines play important roles in stem cell homing. Here we investigated molecular targets to be utilized in modulating the mobility of endogenous CSCs. In a four week follow-up after experimental acute myocardial infarction (AMI) with ligation of the left anterior descending (LAD) coronary artery of Sprague-Dawley rats c-Kit+ CSCs redistributed in the heart...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29344240/cxcl5-as-an-autocrine-or-paracrine-cytokine-is-associated-with-proliferation-and-migration-of-hepatoblastoma-hepg2-cells
#19
Yang Yang, Jie Hou, Mingliang Shao, Wei Zhang, Yaling Qi, Shengnan E, Shuqiu Wang, Hongyu Sui, Dexin Meng, Baixin Wang, Mingfu Wang, Yang Han, Yu Cao, Xiaoqing Huang, Yue Li, Pengxia Zhang, Weiqun Wang
C-X-C motif chemokine ligand 5 (CXCL5) is a CXC-type chemokine that is a crucial inflammatory mediator and a powerful attractant for granulocytic immune cells. Increasing evidence has indicated that CXCL5 is involved in the tumorigenesis of various malignancies. The present investigation demonstrated that CXCL5 was expressed in both hepatoblastoma HepG2 cells and liver stellate LX-2 cells, and CXCL5's receptor C-X-C chemokine receptor type 2 (CXCR2) was expressed in HepG2 cells by reverse transcription-polymerase chain reaction (RT-PCR), western blotting and ELISA assays...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29258556/lysophosphatidic-acid-via-lpa-receptor-5-protein-kinase-d-dependent-pathways-induces-a-motile-and-pro-inflammatory-microglial-phenotype
#20
I Plastira, E Bernhart, M Goeritzer, T DeVaney, H Reicher, A Hammer, B Lohberger, A Wintersperger, B Zucol, W F Graier, D Kratky, E Malle, W Sattler
BACKGROUND: Extracellular lysophosphatidic acid (LPA) species transmit signals via six different G protein-coupled receptors (LPAR1-6) and are indispensible for brain development and function of the nervous system. However, under neuroinflammatory conditions or brain damage, LPA levels increase, thereby inducing signaling cascades that counteract brain function. We describe a critical role for 1-oleyl-2-hydroxy-sn-glycero-3-phosphate (termed "LPA" throughout our study) in mediating a motile and pro-inflammatory microglial phenotype via LPAR5 that couples to protein kinase D (PKD)-mediated pathways...
December 19, 2017: Journal of Neuroinflammation
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