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Sven Rutkowski, Ling Mu, Tieyan Si, Meiyu Gai, Mengmeng Sun, Johannes Frueh, Qiang He
One of the most promising future applications of hydrogels is drug delivery. The hydrogels act as a biomedical cargo model to reach the target and release drugs to cure diseases. This application requires no side effects of the hydrogel and the ability to pass through porous media (e.g. membranes, interstitial tissue etc.) with nanoscaled channels. At the same time, the hydrogel must be mass-producible in an economic way. In this work, we show that hydrodynamic electrospray ionization jetting combined with ultrasound can fulfill these high requirements...
November 28, 2018: Colloids and Surfaces. B, Biointerfaces
Min Yang, Nannan Li, Suxiao Yang, Yuan Yu, Zhenlian Han, Li Li, Haijin Mou
Understanding the effect of conserved domains reconstruction of alginate lyases on action mode is essential for their application and in-depth study. We report the expression and action mode of recombinant alginate lyase (AlyM) and its conserved domain reconstruction forms (AlyMΔCBM, cAlyM, and AlyMΔ58C). The enzymatic activities of AlyM, AlyMΔCBM, cAlyM, and AlyMΔ58C were 61.77, 150.57, 388.97, and 308.21 U/mg, respectively. The transcription level of cAlyM was 49.89-fold of AlyM. cAlyM and AlyMΔ58C showed higher thermal stability than AlyM, indicating that the removal of F5_F8_type_C domain was beneficial for the increase of thermal stability...
December 4, 2018: Applied Microbiology and Biotechnology
Özge Lalegül-Ülker, Şükran Şeker, Ayşe Eser Elçin, Yaşar Murat Elçin
Repair of volumetric muscle loss (VML) injuries is a complicated endeavour which necessitates the collaborative use of different regenerative approaches and technologies. Herein is proposed the development of fibrin-based microbeads (FMs) alone or as a bone marrow mesenchymal stem cell (MSC) encapsulation matrix for modular muscle engineering. FMs were generated through the ionotropic gelation of alginate and fibrinogen obtained from the platelet-rich plasma of whole blood, and then removing the alginate by citrate treatment...
December 4, 2018: Artificial Cells, Nanomedicine, and Biotechnology
C P Devatha, N Pavithra
Triclosan (TCS) is a well-known emerging contaminant got wide use in daily use products of domestic purpose, which provides the way to enter the ecological cycle, and is preferably detected in sewage treatment plants. In this study, TCS degrading bacteria (TDB) was isolated and identified from a wastewater treatment plant at the National Institute of Technology-Karnataka, Surathkal (NITK), India. The isolate was reported as Pseudomonas strain by performing 16S RNA Sequencing using BLAST analysis. Bacterial growth depends upon several environmental factors...
December 1, 2018: Journal of Environmental Management
Natália Noronha Ferreira, Bruno Leonardo Caetano, Fernanda Isadora Boni, Flávia Sousa, Marina Magnani, Bruno Sarmento, Beatriz Stringhetti Ferreira Cury, Maria Palmira Daflon Gremião
Alginate-based polyelectrolytes complexes (PECs) and hydrogel were engineered as platforms for local bevacizumab (BVZ) therapy. This study provides deep comprehension on the microstructures of such systems, and their correlation with drug release patterns. PECs and hydrogel were characterized using Fourier transform infrared spectroscopy (FTIR), small-angle X-ray scattering (SAXS), scanning electron microscopy (SEM), atomic force microscopy (AFM) and porosimetry. Structural investigations indicated that PECs are formed by supramolecular interactions, resulting in physically crosslinked polymer networks, while the bevacizumab-loaded hydrogel has a more compact and rigid structure, promoting better entrapment of BVZ...
December 1, 2018: Journal of Pharmaceutical Sciences
Clive J Curley, Eimear B Dolan, Matthias Otten, Svenja Hinderer, Garry P Duffy, Bruce P Murphy
As treatments for myocardial infarction (MI) continue to improve, the population of people suffering from heart failure (HF) is rising significantly. Novel treatment strategies aimed at achieving long-term functional stabilisation and improvement in heart function post MI include the delivery of biomaterial hydrogels and myocardial matrix-based therapies to the left ventricle wall. Individually alginate hydrogels and myocardial matrix-based therapies are at the most advanced stages of commercial/clinical development for this potential treatment option...
December 3, 2018: Drug Delivery and Translational Research
Steffen Sydow, Dominik de Cassan, Robert Hänsch, Thomas R Gengenbach, Christopher D Easton, Helmut Thissen, Henning Menzel
Nanogels were prepared by ionotropic gelation of chitosan (CS) with tripolyphosphate (TPP). The use of such nanogels to prepare coatings by layer-by-layer deposition (LbL) was studied. The nanogels were characterized in terms of particle size, zeta-potential and stability. Nanogel suspensions were used to build polyelectrolyte multilayers on silicon wafers and on PCL fiber mats by LbL-deposition. Three different polysaccharides were used as polyanions, namely chondroitin sulfate, alginate and hyaluronic acid...
December 4, 2018: Biomaterials Science
Chutamart Pitakchatwong, Suwabun Chirachanchai
The fabrication of polysaccharides to be nanoparticles or nanocapsules is quite specific due to various parameters and factors. The present work demonstrates a simple pathway to selectively prepare the ionic polysaccharide flakes to be nanoparticles or nanocapsules. The systematic studies on the model cases of cationic polysaccharide (i.e. chitosan) and anionic polysaccharide (i.e. alginate) confirm that pKa is the key point to tune the polysaccharides to be nanoparticles or nanocapsules. When the ionic polysaccharides were in an oil/water emulsion system, the pH close to pKa leads to the densely packed polysaccharide chains under the hydrogen bond networks, and as a result the crosslink occurs all through the chains to be nanoparticles...
December 3, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Barry Snow, Eoin Mulroy, Arnold Bok, Mark Simpson, Andrew Smith, Kenneth Taylor, Michelle Lockhart, Bb Janice Lam, Christopher Frampton, Patrick Schweder, Benson Chen, Gregory Finucane, Adele McMahon, Lorraine Macdonald
INTRODUCTION: Regenerative therapies in Parkinson's disease aim to slow neurodegeneration and re-establish damaged neuronal circuitry. Neurotrophins are potent endogenous regulators of neuronal survival, development and regeneration. They represent an attractive regenerative treatment option in Parkinson's disease. Porcine choroid plexus produces a number of neurotrophins, and can be safely delivered to the striatum in an encapsulated formulation (termed NTCELL® ) to protect them from immune attack...
November 13, 2018: Parkinsonism & related Disorders
Pongsatorn Taweetanawanit, Thunyalux Ratpukdi, Sumana Siripattanakul-Ratpukdi
This study investigated removal of triclocarban (TCC) from contaminated wastewater by Pseudomonas fluorescens strain MC46 entrapped in barium alginate. Appropriate entrapped cell preparation conditions (cell-to-entrapment material ratio and cell loading) for removing TCC were examined. The highest TCC removal by the entrapped and free cell systems at the initial TCC concentration of 10 mg/L was 72 and 45%, respectively. TCC was degraded to less toxic compounds. Self-substrate inhibition was found at TCC concentration of 30 mg/L...
November 24, 2018: Bioresource Technology
Juanjuan Qu, Tao Song, Jinsong Liang, Xin Bai, Yue Li, Yingnan Wei, Siqi Huang, Liying Dong, Yu Jin
In this study, Auricularia Matrix Waste (AMW) was modified by sodium hydroxide and immobilized into granular adsorbent with sodium alginate to remove lead ions from aqueous solution through a fixed-bed column. The results of Scanning Electron Microscope-Energy Dispersive X-ray (SEM-EDX) and Fourier Transform Infrared Spectroscopy (FTIR) illuminated that immobilization greatly changed the structure, elements, polarity and functional groups of the adsorbent. Amino, hydroxyl, carboxyl groups on the adsorbent actively participated lead(II) adsorption and cation exchange also played an important role in adsorption process...
November 28, 2018: Ecotoxicology and Environmental Safety
Garmia Derafa, Hassina Zaghouane-Boudiaf
A very effective and low cost bioadsorbents derived from Urtica dioica leaf powder (UDL) and composite beads UDL encapsulated with calcium alginate (UDL/A) were used in removal of cationic dye crystal violet (CV) from aqueous medium. The new adsorbents were characterized by Fourier transform infrared spectroscopy (FTIR) and isoelectric points (pHpzc). Adsorption was studied in batch system according to initial CV concentration (30-150 mg/L), contact time, pH (2-11), temperature (10 to 40 °C) and adsorbent mass (10 to 200 mg)...
November 28, 2018: International Journal of Biological Macromolecules
Noelia Flórez-Fernández, María Dolores Torres, María Jesús González-Muñoz, Herminia Domínguez
The non-isothermal autohydrolysis temperature impact of edible brown seaweed Laminaria ochroleuca was studied to recover high valuable compounds. Extraction yield was determined, above 80% was obtained at 220 °C. The maximal fucose content (17% d.b.) was attained at 180 °C, whereas the maximal sulphate was achieved at 160 °C, and phenolic and protein content at 220 °C. The maximum sulphated fucoidan content (41.38 g fucoidan/100 g extract) was obtained at 160 °C, whereas the maximum fucose oligosaccharides was obtained at 180 °C...
March 30, 2019: Food Chemistry
K Kanimozhi, S KhaleelBasha, V SuganthaKumari, K Kaviyarasu
Sodium Alginate/Poly(vinyl alcohol) (SA/PVA) blend scaffolds were successfully prepared via solution casting method for controlled release of ciprofloxacin (CPF). The structures of the films were evaluated by ATR-FTIR, XRD and SEM. A wide variety of material characteristics for the SA/PVA blend scaffolds were investigated, including the swelling behaviour, liquid displacement method, mechanical property and antibacterial activity. ATR-FTIR studies evaluated the chemical interaction between the biopolymeric scaffolds and the drug...
May 1, 2019: Journal of Nanoscience and Nanotechnology
Shuxian Tang, Ying Zhao, Haitao Wang, Yuqiao Wang, Hexiang Zhu, Yu Chen, Shusen Chen, Shaohua Jin, Ziming Yang, Puwang Li, Sidong Li
A polyampholytic superabsorbent polymer (PASAP), sodium alginate-g-(polyacrylic acid-co-allyltrimethylammonium chloride) (SA-g-(PAA-co-PTM)), was prepared by free-radical graft copolymerization and characterized. The polymer exhibited pH-dependent swelling behaviors with extremely high swelling ratios, and was saline tolerant. The dye adsorption properties of SA-g-(PAA-co-PTM) were investigated using methylene blue (MB) as a cationic dye model. It was found that its dye adsorption capacity was significantly affected by the TM content in PASAP and pH of dye solution...
November 29, 2018: Marine Drugs
A S Montaser, Mohamed Rehan, Mehrez E El Nagar
Skin re-infection problems and its correlation with inflammation, causes rising wound temperature resulting wound healing impairment. In this current study, polyvinyl alcohol (PVA) hydrogel blended with thermosensitive sodium alginate-g-N-isopropyl acrylamide (SA-g-NIPAM) for producing thermosensitive hydrogel (PVA/SA-g-NIPAM) for controlled response to re-infected wounds. The thermosensitive SA-g-NIPAM was prepared using redox copolymerization with NIPAM. The fabricated (PVA/SA-g-NIPAM) grafted copolymer was investigated via FTIR technique...
November 27, 2018: International Journal of Biological Macromolecules
Xiaoliu Huangfu, Chengxue Ma, Ruixing Huang, Qiang He, Caihong Liu, Jian Zhou, Jin Jiang, Jun Ma, Yinying Zhu, Muhua Huang
The initial deposition kinetics of colloidal MnO2 on three representative surfaces in aquatic systems (i.e., silica, magnetite, and alumina) in NaNO3 solution were investigated in the presence of model constituents, including humic acid (HA), a polysaccharide (alginate), and a protein (bovine serum albumin (BSA), using laboratory quartz crystal microbalance with dissipation monitoring equipment (QCM-D). The results indicated that the deposition behaviors of MnO2 colloids on three surfaces were in good agreement with classical Derjaguin-Landau-Verwey-Overbeek (DLVO) theory...
November 30, 2018: Environmental Science & Technology
Kira Pusch, Thomas J Hinton, Adam W Feinberg
Syringe pump extruders are required for a wide range of 3D printing applications, including bioprinting, embedded printing, and food printing. However, the mass of the syringe becomes a major challenge for most printing platforms, requiring compromises in speed, resolution and/or volume. To address these issues, we have designed a syringe pump large volume extruder (LVE) that is compatible with low-cost, open source 3D printers, and herein demonstrate its performance on a PrintrBot Simple Metal. Key aspects of the LVE include: (1) it is open source and compatible with open source hardware and software, making it inexpensive and widely accessible to the 3D printing community, (2) it utilizes a standard 60 mL syringe as its ink reservoir, effectively increasing print volume of the average bioprinter, (3) it is capable of retraction and high speed movements, and (4) it can print fluids using nozzle diameters as small as 100 µm, enabling the printing of complex shapes/objects when used in conjunction with the freeform reversible embedding of suspended hydrogels (FRESH) 3D printing method...
April 2018: HardwareX
Xiong Wang, Xinzhi Zhang, Xingliang Dai, Xuanzhi Wang, Xinda Li, Jinfu Diao, Tao Xu
Currently, there is still a lack of appropriate in vitro model for studying lung cancers, especially for recapitulating their invasion and metastasis properties. To develop an appropriate in vitro model for lung cancer research, low-temperature molding principle of biological manufacturing and 3D bioprinting was used in this study to fabricate a cell-laden hydrogel grid scaffold structure, using gelatin-sodium alginate-lung cancer cell A549/95-D suspension as the bio-ink. Cells distributed evenly in this model with high viability, and can be cultured sustainably...
December 2018: 3 Biotech
Youran Li, Hanrong Wang, Jiawei Lu, Alex Chu, Liang Zhang, Zhongyang Ding, Sha Xu, Zhenghua Gu, Guiyang Shi
The application of immobilized lipase in the enzymatic production of biodiesel has shown numerous advantages. In this study, surface of Polyacrylonitrile (PAN) hollow membrane was first modified using nitrile-click chemistry in order to fit for interaction with enzyme proteins. Then sodium alginate (SA) was introduced and the membrane was post-treated by CaCl2 . When the prepared PAN-PEI-SA-CaCl2 was used for lipase immobilization, the protein loading was 36.90 mg/g, and the enzyme activity reached up to 54...
November 22, 2018: Bioresource Technology
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