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https://www.readbyqxmd.com/read/28927258/a-proteomics-based-approach-reveals-differential-regulation-of-visceral-adipose-tissue-proteins-between-metabolically-healthy-and-unhealthy-obese-patients
#1
Assim A Alfadda, Afshan Masood, Mohammed Y Al-Naami, Pierre Chaurand, Hicham Benabdelkamel
Obesity and the metabolic disorders that constitute metabolic syndrome are a primary cause of morbidity and mortality in the world. Nonetheless, the changes in the proteins and the underlying molecular pathways involved in the relevant pathogenesis are poorly understood.In this study a proteomic analysis of the visceral adipose tissue isolated from metabolically healthy and unhealthy obese patients was used to identify presence of altered pathway(s) leading to metabolic dysfunction. Samples were obtained from 18 obese patients undergoing bariatric surgery and were subdivided into two groups based on the presence or absence of comorbidities as defined by the International Diabetes Federation...
September 20, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28927239/tubulins-rhythms-and-cell-walls-in-poplar-leaves-it-s-all-in-the-timing
#2
Scott A Harding, Hao Hu, Batbayar Nyamdari, Liang-Jiao Xue, Radnaa Naran, Chung-Jui Tsai
Plant cell walls exhibit architectural and compositional changes throughout their development and in response to external cues. While tubulins are involved in cell wall biogenesis, much remains unknown about the scope of their involvement during the orchestration of this resource-demanding process. A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis...
September 12, 2017: Tree Physiology
https://www.readbyqxmd.com/read/28927238/factor-v-leiden-is-associated-with-increased-sperm-count
#3
T E van Mens, U N Joensen, Z Bochdanovits, A Takizawa, J Peter, N Jørgensen, P B Szecsi, J C M Meijers, H Weiler, E Rajpert-De Meyts, S Repping, S Middeldorp
STUDY QUESTION: Is the thrombophilia mutation factor V Leiden (FVL) associated with an increased total sperm count? SUMMARY ANSWER: Carriers of FVL have a higher total sperm count than non-FVL-carriers, which could not be explained by genetic linkage or by observations in a FVL-mouse model. WHAT IS KNOWN ALREADY: FVL has a high prevalence in Caucasians despite detrimental health effects. Carriers have been shown to have higher fecundity, which might partly explain this evolutionary paradox...
September 12, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28927140/itraq-coupled-2d-lc-ms-ms-analysis-of-cxcr7-transfected-papillary-thyroid-carcinoma-cells-a-new-insight-into-cxcr7-regulation-of-papillary-thyroid-carcinoma-progression-and-identification-of-potential-biomarkers
#4
Hengwei Zhang, Lei Yang, Zhangyi Liu, Chenxi Liu, Xuyong Teng, Lei Zhang, Bo Yin, Zhen Liu
Previous studies have demonstrated that C-X-C chemokine receptor type 7 (CXCR7) regulates papillary thyroid carcinoma (PTC) growth and metastasis; however, the molecular mechanisms underlying this regulation remain unclear. In the present study, the protein expression profiles of the PTC cell line GLAG-66 and GLAG-66 cells stably transfected with CXCR7 cDNA were analyzed and compared using isobaric tag for relative and absolute quantification-coupled two-dimensional liquid chromatography-tandem mass spectrometry...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28926930/modeling-of-bn-lifetime-prediction-of-a-system-based-on-integrated-multi-level-information
#5
Jingbin Wang, Xiaohong Wang, Lizhi Wang
Predicting system lifetime is important to ensure safe and reliable operation of products, which requires integrated modeling based on multi-level, multi-sensor information. However, lifetime characteristics of equipment in a system are different and failure mechanisms are inter-coupled, which leads to complex logical correlations and the lack of a uniform lifetime measure. Based on a Bayesian network (BN), a lifetime prediction method for systems that combine multi-level sensor information is proposed. The method considers the correlation between accidental failures and degradation failure mechanisms, and achieves system modeling and lifetime prediction under complex logic correlations...
September 15, 2017: Sensors
https://www.readbyqxmd.com/read/28926823/preparation-characterization-and-application-of-urease-nanoparticles-for-construction-of-an-improved-potentiometric-urea-biosensor
#6
Seema Jakhar, C S Pundir
The nanoparticles (NPs) aggregates of commercial urease from jack beans (Canavalia ensiformis) were prepared by desolvation and glutaraldehyde crosslinking and functionalized by cysteamine dihydrochloride. These enzyme nanoparticles (ENPs) were characterized by transmission electron microscopy (TEM), UV and Fourier transform infrared (FTIR) spectroscopy. The TEM images of urease NPs showed their size in the range, 18-100nm with an average of 51.2nm. The ENPs were more active and stable with a longer shelf life than native enzyme molecules...
September 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28926811/towards-better-monitoring-of-technology-critical-elements-in-europe-coupling-of-natural-and-anthropogenic-cycles
#7
Philip Nuss, Gian Andrea Blengini
The characterization of elemental cycles has a rich history in biogeochemistry. Well known examples include the global carbon cycle, or the cycles of the 'grand nutrients' nitrogen, phosphorus, and sulfur. More recently, efforts have increased to better understand the natural cycling of technology critical elements (TCEs), i.e. elements with a high supply risk and economic importance in the EU. On the other hand, tools such as material-flow analysis (MFA) can help to understand how substances and goods are transported and accumulated in man-made technological systems ('anthroposphere')...
September 16, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28926741/cellular-glutathione-levels-in-hl-60-cells-during-respiratory-burst-are-not-correlated-with-ultra-weak-photon-emission
#8
Rosilene Cristina Rossetto Burgos, Wei Zhang, Eduard P A van Wijk, Thomas Hankemeier, Rawi Ramautar, Jan van der Greef
Recently, ultra-weak photon emission (UPE) was developed as a novel tool for measuring oxidative metabolic processes, as its generation is related to reactive oxygen species (ROS). Both an imbalance in ROS or the uncontrolled production of ROS can lead to oxidative stress, which is commonly associated with many diseases. In addition to playing several biological functions, the thiol amino acid glutathione has an important antioxidant function in the body's defense against ROS. Specifically, glutathione is an important endogenous antioxidant that helps maintain oxidant levels...
September 7, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28926735/ketamine-metabolites-with-antidepressant-effects-fast-economical-and-eco-friendly-enantioselective-separation-based-on-supercritical-fluid-chromatography-sfc-and-single-quadrupole-ms-detection
#9
Georg M Fassauer, Robert Hofstetter, Mahmoud Hasan, Stefan Oswald, Christina Modeß, Werner Siegmund, Andreas Link
Increasing evidence accumulates that metabolites of the dissociative anesthetic ketamine contribute considerably to the biological effects of this drug and could be developed as next generation antidepressants, especially for acute treatment of patients with therapy-refractory major depression. Analytical methods for the simultaneous determination of the plethora of hydroxylated, dehydrogenated and/or demethylated compounds formed after administration of ketamine hydrochloride are a prerequisite for future clinical investigations and a deeper understanding of the individual role of the isomers of these metabolites...
September 8, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28926712/pyrophosphate-release-in-the-protein-hiv-reverse-transcriptase
#10
Murat Atiş, Kenneth A Johnson, Ron Elber
Enzymatic reactions usually occur in several steps: A step of substrate binding to the surface of the protein, a step of protein re-organization around the substrate and conduction of a chemical reaction, and a step of product release. The release of inorganic phosphate - PPi - from the matrix of the protein HIV reverse transcriptase is investigated computationally. Atomically detailed simulations with explicit solvent are analyzed to obtain the free energy profile, mean first passage time, and detailed molecular mechanisms of PPi escape...
September 19, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28926705/a-low-temperature-transition-metal-free-cross-dehydrogenative-coupling-protocol-for-the-synthesis-of-3-3-disubstituted-oxindoles
#11
James R Donald, Richard John Kenneth Taylor, Wade F Petersen
A new low-temperature procedure for the synthesis of 3,3-disubstituted 2-oxindoles via cross dehydrogenative coupling (CDC) is reported. The use of a strong, non-reversible base in these reactions has been found to effect a dramatic drop in reaction temperature (to room temperature) relative to the current state-of-the-art (>100 °C). When employing iodine as an 'oxidant', new evidence suggests that this transformation may occur via a transiently stable iodinated intermediate rather than by direct single-electron oxidation...
September 19, 2017: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/28926701/forensic-sampling-and-analysis-from-a-single-substrate-for-surface-enhanced-raman-spectroscopy-followed-by-paper-spray-mass-spectrometry
#12
Patrick W Fedick, Brandon J Bills, Nicholas E Manicke, R Graham Cooks
Sample preparation is the most common bottle-neck in the analysis and processing of forensic evidence. Time-consuming steps in many forensic tests involve complex separations such as liquid and gas chromatography or various types of extraction techniques, typically coupled with mass spectrometry (e.g. LC-MS). Ambient ionization ameliorates these slow steps by reducing or even eliminating sample preparation. While some ambient ionization techniques have been adopted by the forensic community, there is significant resistance to discarding chromatography as most forensic analyses require both an identification and a confirmation technique...
September 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28926698/quantification-of-lignin-and-its-structural-features-in-plant-biomass-using-13-c-lignin-as-internal-standard-for-pyrolysis-gc-sim-ms
#13
Gijs van Erven, Ries de Visser, Donny W H Merkx, Willem Strolenberg, Peter De Gijsel, Harry Gruppen, Mirjam A Kabel
Understanding mechanisms underlying plant biomass recalcitrance at molecular level can only be achieved by accurate analyses of both content and structural features of the molecules involved. Current quantification of lignin is, however, majorly based on unspecific gravimetric analysis after sulphuric acid hydrolysis. Hence, our research aimed at specific lignin quantification with concurrent characterization of its structural features. Hereto, for the first time, a polymeric (13)C lignin was used as internal standard (IS) for lignin quantification via analytical pyrolysis coupled to gas chromatography with mass-spectrometric detection in selected ion monitoring mode (py-GC-SIM-MS)...
September 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28926682/development-of-a-simple-and-valid-method-for-the-trace-determination-of-phthalate-esters-in-human-plasma-using-dispersive-liquid-liquid-microextraction-coupled-with-gc-ms
#14
Karim Ebrahim, Parinaz Poursafa, Mohammad Mehdi Amin
A new method was developed for the trace determination of phthalic acid esters in plasma using dispersive liquid-liquid microextraction and gas chromatography with mass spectrometry analysis. Plasma proteins were efficiently precipitated by trichloroacetic acid and then a mixture of chlorobenzene (as extraction solvent) and acetonitrile (as dispersive solvent) rapidly injected to clear supernatant using a syringe. After the centrifuging, chlorobenzene were sedimented in the bottom of the test tube. 1 μL of this sedimented phase was injected into the gas chromatograph for phthalic acid esters analysis...
September 19, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28926564/probabilistic-models-for-neural-populations-that-naturally-capture-global-coupling-and-criticality
#15
Jan Humplik, Gašper Tkačik
Advances in multi-unit recordings pave the way for statistical modeling of activity patterns in large neural populations. Recent studies have shown that the summed activity of all neurons strongly shapes the population response. A separate recent finding has been that neural populations also exhibit criticality, an anomalously large dynamic range for the probabilities of different population activity patterns. Motivated by these two observations, we introduce a class of probabilistic models which takes into account the prior knowledge that the neural population could be globally coupled and close to critical...
September 19, 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28926402/reversing-hiv-latency-via-sphingosine-1-phosphate-receptor-1-signaling
#16
Charline Duquenne, Sandrine Gimenez, Adeline Guigues, Benjamin Viala, Caroline Boulouis, Clément Mettling, Damien Maurel, Noëlie Campos, Etienne Doumazane, Laetitia Comps-Agrar, Jamal Tazi, Laurent Prézeau, Pierre Corbeau, Vincent François
OBJECTIVE: In this study, we looked for a new family of latency reversing agents. DESIGN: We searched for G protein-coupled receptors (GPCR) coexpressed with CCR5 in primary CD4+ T cells that activate infected cells and boost HIV production. METHODS: GPCR coexpression was unveiled by RT-PCR. We used FRET to analyze the dimerization with CCR5 of the expressed GPCR. Viral entry was measured by flow cytometry, reverse transcription by quantitative PCR, NFkB translocation by immunofluorescence, LTR activation using a gene reporter assay, and viral production by p24 quantification...
September 18, 2017: AIDS
https://www.readbyqxmd.com/read/28926328/-morbidity-in-the-dependent-elderly-cared-by-home-teams-of-the-national-network-of-continued-integrated-care-in-the-lisbon-and-tagus-valley-cross-sectional-study
#17
Paula Broeiro-Gonçalves
INTRODUCTION: In Portugal, the National Network of Continuing Integrated Care's mission is to take care of new health and social needs. The aim of the study was to know the disease burden and disability of the elderly (75 and over) cared by the integrated continuing care teams. MATERIAL AND METHODS: A cross-sectional study carried out in a sample of 230 participants, from 25 teams randomly selected in the region of Lisbon and Tagus Valley. Data were collected at the patient's home trough caregiver's interviewing...
August 31, 2017: Acta Médica Portuguesa
https://www.readbyqxmd.com/read/28926272/collective-lasing-behavior-of-monolithic-gan-ingan-core-shell-nanorod-lattice-under-room-temperature
#18
Chia-Yen Huang, Jing-Jie Lin, Tsu-Chi Chang, Che-Yu Liu, Tzu-Ying Tai, Kuo-Bin Hong, Tien-Chang Lu, Hao-Chung Kuo
We demonstrated a monolithic GaN-InGaN core-shell nanorod lattice lasing under room temperature. The threshold pumping density was as low as 140 kW/cm2 with a quality factor as high as 1940. The narrow mode spacing between lasing peaks suggested a strong coupling between adjacent whisper gallery modes (WGM), which was confirmed with the far-field patterns. Excitation area dependent photoluminescence revealed that the long-wavelength lasing modes dominated the collective lasing behavior under a large excitation area...
September 19, 2017: Nano Letters
https://www.readbyqxmd.com/read/28926268/direct-access-to-cobaltacycles-via-c-h-activation-n-chloroamide-enabled-room-temperature-synthesis-of-heterocycles
#19
Xiaolong Yu, Kehao Chen, Shan Guo, Pengfei Shi, Chao Song, Jin Zhu
Cobaltacycle synthesis via C-H activation has been achieved for the first time, providing key mechanistic insight into cobalt catalytic chemistry. N-Chloroamides are used as a directing synthon for cobalt-catalyzed room-temperature C-H activation and construction of heterocycles. Alkynes as coupling partners allow convenient access to isoquinolones, a class of synthetically and pharmaceutically important compounds. The broad substrate scope enables a diverse range of substitution patterns to be incorporated into the heterocyclic scaffold...
September 19, 2017: Organic Letters
https://www.readbyqxmd.com/read/28926267/synthesis-and-analysis-of-bacterial-folate-metabolism-intermediates-and-antifolates
#20
Surendra Dawadi, Shannon L Kordus, Anthony D Baughn, Courtney C Aldrich
The mechanism of action of para-aminosalicylic acid (PAS), a drug used to treat drug-resistant tuberculosis (TB), has been confirmed through the first synthesis and biochemical characterization of its active metabolite 7. The synthesis features the coupling of N(2)-acetyl-6-formylpterin obtained from the degradation of folic acid and appropriately functionalized arylamines to form Schiff bases. The sequential chemoselective reduction of the imine and pterin ring led to the formation of dihydrofolate analogue 7 and two other dihydropteroate species...
September 19, 2017: Organic Letters
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