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Gut Virome

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https://www.readbyqxmd.com/read/30472682/enteric-fungal-microbiota-dysbiosis-and-ecological-alterations-in-colorectal-cancer
#1
Olabisi Oluwabukola Coker, Geicho Nakatsu, Rudin Zhenwei Dai, William Ka Kei Wu, Sunny Hei Wong, Siew Chien Ng, Francis Ka Leung Chan, Joseph Jao Yiu Sung, Jun Yu
OBJECTIVES: Bacteriome and virome alterations are associated with colorectal cancer (CRC). Nevertheless, the gut fungal microbiota in CRC remains largely unexplored. We aimed to characterise enteric mycobiome in CRC. DESIGN: Faecal shotgun metagenomic sequences of 184 patients with CRC, 197 patients with adenoma and 204 control subjects from Hong Kong were analysed (discovery cohort: 73 patients with CRC and 92 control subjects; validation cohort: 111 patients with CRC, 197 patients with adenoma and 112 controls from Hong Kong)...
November 24, 2018: Gut
https://www.readbyqxmd.com/read/30459201/diagnostic-potential-and-interactive-dynamics-of-the-colorectal-cancer-virome
#2
Geoffrey D Hannigan, Melissa B Duhaime, Mack T Ruffin, Charlie C Koumpouras, Patrick D Schloss
Human viruses (those that infect human cells) have been associated with many cancers, largely due to their mutagenic and functionally manipulative abilities. Despite this, cancer microbiome studies have focused almost exclusively on bacteria instead of viruses. We began evaluating the cancer virome by focusing on colorectal cancer, a primary cause of morbidity and mortality throughout the world and a cancer linked to altered colonic bacterial community compositions but with an unknown association with the gut virome...
November 20, 2018: MBio
https://www.readbyqxmd.com/read/30449316/biology-and-taxonomy-of-crass-like-bacteriophages-the-most-abundant-virus-in-the-human-gut
#3
Emma Guerin, Andrey Shkoporov, Stephen R Stockdale, Adam G Clooney, Feargal J Ryan, Thomas D S Sutton, Lorraine A Draper, Enrique Gonzalez-Tortuero, R Paul Ross, Colin Hill
CrAssphages represent the most abundant virus in the human gut microbiota, but the lack of available genome sequences for comparison has kept them enigmatic. Recently, sequence-based classification of distantly related crAss-like phages from multiple environments was reported, leading to a proposed familial-level taxonomic group. Here, we assembled the metagenomic sequencing reads from 702 human fecal virome/phageome samples and analyzed 99 complete circular crAss-like phage genomes and 150 contigs ≥70 kb...
November 14, 2018: Cell Host & Microbe
https://www.readbyqxmd.com/read/30429469/%C3%AE-crass001-represents-the-most-abundant-bacteriophage-family-in-the-human-gut-and-infects-bacteroides-intestinalis
#4
Andrey N Shkoporov, Ekaterina V Khokhlova, C Brian Fitzgerald, Stephen R Stockdale, Lorraine A Draper, R Paul Ross, Colin Hill
CrAssphages are an extensive and ubiquitous family of tailed bacteriophages, predicted to infect bacteria of the order Bacteroidales. Despite being found in ~50% of individuals and representing up to 90% of human gut viromes, members of this viral family have never been isolated in culture and remain understudied. Here, we report the isolation of a CrAssphage (ΦCrAss001) from human faecal material. This bacteriophage infects the human gut symbiont Bacteroides intestinalis, confirming previous in silico predictions of the likely host...
November 14, 2018: Nature Communications
https://www.readbyqxmd.com/read/30356736/immunological-tolerance-and-function-associations-between-intestinal-bacteria-probiotics-prebiotics-and-phages
#5
REVIEW
Luis Vitetta, Gemma Vitetta, Sean Hall
Post-birth there is a bacterial assault on all mucosal surfaces. The intestinal microbiome is an important participant in health and disease. The pattern of composition and concentration of the intestinal microbiome varies greatly. Therefore, achieving immunological tolerance in the first 3-4 years of life is critical for maintaining health throughout a lifetime. Probiotic bacteria are organisms that afford beneficial health effects to the host and in certain instances may protect against the development of disease...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/30302521/alterations-in-the-diversity-and-composition-of-mice-gut-microbiota-by-lytic-or-temperate-gut-phage-treatment
#6
Hong-Duo Bao, Mao-da Pang, Ademola Olaniran, Xu-Hui Zhang, Hui Zhang, Yan Zhou, Li-Chang Sun, Stefan Schmidt, Ran Wang
Phages, the most abundant species in the mammalian gut, have numerous advantages as biocontrol agent over antibiotics. In this study, mice were orally treated with the lytic gut phage PA13076 (group B), the temperate phage BP96115 (group C), no phage (group A), or streptomycin (group D) over 31 days. At the end of the experiment, fecal microbiota diversity and composition was determined and compared using high-throughput sequencing of the V3-V4 hyper-variable region of the 16S rRNA gene and virus-like particles (VLPs) were quantified in feces...
October 9, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30252582/metagenomic-analysis-of-intestinal-mucosa-revealed-a-specific-eukaryotic-gut-virome-signature-in-early-diagnosed-inflammatory-bowel-disease
#7
Federica Ungaro, Luca Massimino, Federica Furfaro, Valeria Rimoldi, Laurent Peyrin-Biroulet, Silvia D'Alessio, Silvio Danese
Intestinal dysbiosis is one of the causes underlying the pathogenesis of inflammatory bowel disease (IBD), encompassing ulcerative colitis (UC) and Crohn's disease (CD). Besides bacteria, microbiota comprises both prokaryotic and eukaryotic viruses, that together compose the gut virome. Few works have defined the viral composition of stools, while the virome populating intestinal mucosae from early-diagnosed IBD patients has never been studied. Here we show that, by in-depth metagenomic analysis of RNA-Seq data obtained from gut mucosae of young treatment-naïve patients, early-diagnosed for CD and UC, and from healthy subjects (Ctrl), UC patients display significantly higher levels of eukaryotic Hepadnaviridae transcripts by comparison with both Ctrl and CD patients, whereas CD patients show increased abundance of Hepeviridae versus Ctrl...
September 25, 2018: Gut Microbes
https://www.readbyqxmd.com/read/30181300/the-intestinal-virome-and-immunity
#8
REVIEW
Jessica A Neil, Ken Cadwell
The composition of the human microbiome is considered a major source of interindividual variation in immunity and, by extension, susceptibility to diseases. Intestinal bacteria have been the major focus of research. However, diverse communities of viruses that infect microbes and the animal host cohabitate the gastrointestinal tract and collectively constitute the gut virome. Although viruses are typically investigated as pathogens, recent studies highlight a relationship between the host and animal viruses in the gut that is more akin to host-microbiome interactions and includes both beneficial and detrimental outcomes for the host...
September 15, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/30169455/enteric-virome-and-bacterial-microbiota-in-children-with-ulcerative-colitis-and-crohn-s-disease
#9
Melissa A Fernandes, Sofia G Verstraete, Tung G Phan, Xutao Deng, Emily Stekol, Brandon LaMere, Susan V Lynch, Melvin B Heyman, Eric Delwart
OBJECTIVES: We examined the fecal enteric virome and bacterial community composition of children with Crohn's disease (CD), ulcerative colitis (UC), and healthy controls to test the hypothesis that unique patterns of viral organisms and/or presence of bacterial pathogens may be identified that could contribute to the pathogenesis of pediatric inflammatory bowel disease (IBD). METHODS: Fecal samples from 24 children (mean 12.2 years) with CD (n = 7) or UC (n = 5) and similar aged controls (n = 12) were processed to determine individual viromes...
August 30, 2018: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/30128999/diversity-analysis-in-viral-metagenomes
#10
Jorge Francisco Vázquez-Castellanos
Viruses are the most abundant and diverse biological entity in the earth. Nowadays, there are several viral metagenomes from different ecological niches which have been used to characterize new viral particles and to determine their diversity. However, viral metagenomic data have the disadvantage to be high-dimensional compositional and sparse. This type of data renders many of the conventional multivariate statistical analyses inoperative. Fortunately, different libraries and statistical packages have been developed to deal with this problem and perform the different ecological and statistical analyses...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/30128992/viral-genome-isolation-from-human-faeces-for-succession-assessment-of-the-human-gut-virome
#11
David Mayo-Muñoz
Despite the important role of the microbiota in the human gastrointestinal tract (GIT) and its impact on life-long health, the successional process through which this microbial community develops during infancy is still poorly understood. Specially, little is known about how the amount and type of viruses present in the GIT, i.e., the virome, varies throughout this period and about the role this collection of viruses may play in the assembly of the GIT microbiota.The patterns of taxonomic change of the GIT viral community can be analyzed in a birth cohort of infants during the first year of life...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/30128991/netovir-modular-approach-to-customize-sample-preparation-procedures-for-viral-metagenomics
#12
Nádia Conceição-Neto, Kwe Claude Yinda, Marc Van Ranst, Jelle Matthijnssens
The democratization of next-generation sequencing (NGS) technologies has enabled scientists to explore the diversity of microbial life in various ecological niches in an unpreceded depth. The role of viruses as a key player in health and disease is becoming increasingly clear. To address the need for an up scalable, reproducible protocol to purify RNA and DNA viruses from a sample, we describe our optimized method. The Novel Enrichment Technique Of Viromes (NetoVIR) allows researchers to attain a fast, reproducible, and high-throughput sample preparation protocol for NGS gut viromics studies...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/30128989/extraction-and-purification-of-viruses-from-fecal-samples-for-metagenome-and-morphology-analyses
#13
Josué L Castro-Mejía, Ling Deng, Finn K Vogensen, Alejandro Reyes, Dennis S Nielsen
The human enteric virome consists of endogenous retro elements and viruses that infect the host and members of the gut microbiome (GM). Mounting evidence suggests that the gut virome plays a central role in maintaining homeostasis and via the GM influences immunology of the host. To thoroughly characterize the gut virome, it is often very useful to first separate and concentrate extracellular viral-like particles (eVLPs) enabling an integrative characterization of them. Here, we describe a detailed protocol for extraction and concentration of the viral fraction from fecal samples based on a polyethylene glycol precipitation (PEG) approach...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/30065169/unprecedented-diversity-of-ssdna-phages-from-the-family-microviridae-detected-within-the-gut-of-a-protochordate-model-organism-ciona-robusta
#14
Alexandria Creasy, Karyna Rosario, Brittany A Leigh, Larry J Dishaw, Mya Breitbart
Phages (viruses that infect bacteria) play important roles in the gut ecosystem through infection of bacterial hosts, yet the gut virome remains poorly characterized. Mammalian gut viromes are dominated by double-stranded DNA (dsDNA) phages belonging to the order Caudovirales and single-stranded DNA (ssDNA) phages belonging to the family Microviridae . Since the relative proportion of each of these phage groups appears to correlate with age and health status in humans, it is critical to understand both ssDNA and dsDNA phages in the gut...
July 31, 2018: Viruses
https://www.readbyqxmd.com/read/30010747/gut-microbial-and-metabolomic-profiles-after-fecal-microbiota-transplantation-in-pediatric-ulcerative-colitis-patients
#15
David J Nusbaum, Fengzhu Sun, Jie Ren, Zifan Zhu, Natalie Ramsy, Nicholas Pervolarakis, Sachin Kunde, Whitney England, Bei Gao, Oliver Fiehn, Sonia Michail, Katrine Whiteson
Ulcerative colitis is a chronic inflammatory disease of the colon that carries a significant disease burden in children. Therefore, new therapeutic approaches are being explored to help children living with this disease. Fecal microbiota transplantation (FMT) has been successful in some children with ulcerative colitis. However, the mechanism of its therapeutic effect in this patient population is not well understood. To characterize changes in gut microbial and metabolomic profiles after FMT, we performed 16S rRNA gene sequencing, shotgun metagenomic sequencing, virome analysis and untargeted metabolomics by gas chromatography-time of flight-mass spectrometry on stool samples collected before and after FMT from four children with ulcerative colitis who responded to this treatment...
September 1, 2018: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/29933546/the-mammalian-intestinal-microbiome-composition-interaction-with-the-immune-system-significance-for-vaccine-efficacy-and-potential-for-disease-therapy
#16
REVIEW
Ulrich Desselberger
The mammalian gut is colonized by a large variety of microbes, collectively termed ‘the microbiome’. The gut microbiome undergoes rapid changes during the first few years of life and is highly variable in adulthood depending on various factors. With the gut being the largest organ of immune responses, the composition of the microbiome of the gut has been found to be correlated with qualitative and quantitative differences of mucosal and systemic immune responses. Animal models have been very useful to unravel the relationship between gut microbiome and immune responses and for the understanding of variations of immune responses to vaccination in different childhood populations...
June 21, 2018: Pathogens
https://www.readbyqxmd.com/read/29844669/responses-of-intestinal-virome-to-silver-nanoparticles-safety-assessment-by-classical-virology-whole-genome-sequencing-and-bioinformatics-approaches
#17
Kuppan Gokulan, Aschalew Z Bekele, Kenneth L Drake, Sangeeta Khare
Background: Effects of silver nanoparticles (AgNP) on the intestinal virome/phage community are mostly unknown. The working hypothesis of this study was that the exposure of pharmaceutical/nanomedicine and other consumer-use material containing silver ions and nanoparticles to the gastrointestinal tract may result in disturbance of the beneficial gut viruses/phages. Methods: This study assesses the impact of AgNP on the survival of individual bacteriophages using classical virology cultivation and electron microscopic techniques...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29761040/viromes-of-one-year-old-infants-reveal-the-impact-of-birth-mode-on-microbiome-diversity
#18
Angela McCann, Feargal J Ryan, Stephen R Stockdale, Marion Dalmasso, Tony Blake, C Anthony Ryan, Catherine Stanton, Susan Mills, Paul R Ross, Colin Hill
Establishing a diverse gut microbiota after birth is being increasingly recognised as important for preventing illnesses later in life. It is well established that bacterial diversity rapidly increases post-partum; however, few studies have examined the infant gut virome/phageome during this developmental period. We performed a metagenomic analysis of 20 infant faecal viromes at one year of age to determine whether spontaneous vaginal delivery (SVD) or caesarean section (CS) influenced viral composition. We find that birth mode results in distinctly different viral communities, with SVD infants having greater viral and bacteriophage diversity...
2018: PeerJ
https://www.readbyqxmd.com/read/29751830/amniotic-fluid-from-healthy-term-pregnancies-does-not-harbor-a-detectable-microbial-community
#19
Efrem S Lim, Cynthia Rodriguez, Lori R Holtz
Recent studies have conflicting data regarding the presence of intra-amniotic microbiota. Viral communities are increasingly recognized as important although overlooked components of the human microbiota. It is unknown if the developing fetus is exposed to a community of viruses (virome). Given the debate over the existence of an intra-amniotic microbial community and the importance of understanding how the infant gut is populated, we characterized the virome and bacterial microbiota of amniotic fluid from 24 uncomplicated term pregnancies using next-generation sequencing methods...
May 11, 2018: Microbiome
https://www.readbyqxmd.com/read/29719871/a-prospective-metagenomic-and-metabolomic-analysis-of-the-impact-of-exercise-and-or-whey-protein-supplementation-on-the-gut-microbiome-of-sedentary-adults
#20
Owen Cronin, Wiley Barton, Peter Skuse, Nicholas C Penney, Isabel Garcia-Perez, Eileen F Murphy, Trevor Woods, Helena Nugent, Aine Fanning, Silvia Melgar, Eanna C Falvey, Elaine Holmes, Paul D Cotter, Orla O'Sullivan, Michael G Molloy, Fergus Shanahan
Many components of modern living exert influence on the resident intestinal microbiota of humans with resultant impact on host health. For example, exercise-associated changes in the diversity, composition, and functional profiles of microbial populations in the gut have been described in cross-sectional studies of habitual athletes. However, this relationship is also affected by changes in diet, such as changes in dietary and supplementary protein consumption, that coincide with exercise. To determine whether increasing physical activity and/or increased protein intake modulates gut microbial composition and function, we prospectively challenged healthy but sedentary adults with a short-term exercise regime, with and without concurrent daily whey protein consumption...
May 2018: MSystems
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