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Single Cell Oil

Xiangpeng Li, Juan Hu, Christopher J Easley
A fully automated droplet generation and analysis device based on pressure driven push-up valves for precise pumping of fluid and volumetric metering has been developed for high resolution hormone secretion sampling and measurement. The device consists of a 3D-printer templated reservoir for single cells or single tissue culturing, a Y-shaped channel for reagents and sample mixing, a T-junction channel for droplet formation, a reference channel to overcome drifts in fluorescence signal, and a long droplet storage channel allowing incubation for homogeneous immunoassays...
August 16, 2018: Lab on a Chip
Daniel Vázquez-Sánchez, Juliana Antunes Galvão, Marina Rodrigues Mazine, Eduardo Micotti Gloria, Marília Oetterer
Effective and environmentally-friendly alternatives to traditional disinfectants are necessary to reduce the pollution and the emergence of antimicrobial-resistant bacterial strains in food-related environments. In the present study, treatments based in single and combined applications of plant essential oils (EOs) were evaluated for control Staphylococcus aureus biofilms. EOs of Lippia sidoides, Thymus vulgaris and Pimenta pseudochariophyllus showed a higher efficacy than peracetic acid and sodium hypochlorite against S...
August 8, 2018: International Journal of Food Microbiology
Tamene Milkessa Jiru, Laurinda Steyn, Carolina Pohl, Dawit Abate
BACKGROUND: Single cell oil has long been considered an alternative to conventional oil sources. The oil produced can also be used as a feedstock for biodiesel production. Oleaginous yeasts have relatively high growth and lipid production rates, can utilize a wide variety of cheap agro-industrial wastes such as molasses, and can accumulate lipids above 20% of their biomass when they are grown in a bioreactor under conditions of controlled excess carbon and nitrogen limitation. RESULTS: In this study, Rhodotorula kratochvilovae (syn, Rhodosporidium kratochvilovae) SY89 was cultivated in a nitrogen-limited medium containing cane molasses as a carbon source...
August 10, 2018: Chemistry Central Journal
David Kennedy, Edward Okello, Paul Chazot, Melanie-Jayne Howes, Samuel Ohiomokhare, Philippa Jackson, Crystal Haskell-Ramsay, Julie Khan, Joanne Forster, Emma Wightman
Extracts of several members of the monoterpene-rich Lamiaceae sub-family Nepetoideae, including those from the Salvia (sage), Melissa ( Lemon balm ) and Rosmarinus (rosemary) genera, evince cognitive and mood effects in humans that are potentially related to their effects on cholinergic and GABAergic neurotransmission. To date, despite promising in vitro properties, the cognitive and mood effects of the closely related Mentha spicata (spearmint) and Mentha piperita (peppermint) remain unexplored. This study therefore assessed the human cognitive/mood effects of the M...
August 7, 2018: Nutrients
Yuanyuan Yao, Ji Ji, Hongding Zhang, Kun Zhang, Baohong Liu, Pengyuan Yang
Single-cell analysis provides an important strategy to evaluate cellular heterogeneity. Although surface-enhanced Raman scattering (SERS) has been considered as a promising label-free technique for single-cell analysis, it remains at the early stage for characterizing the extracellular metabolites of single cells. Herein, we developed a convenient, flexible, and straightforward three-dimensional (3D) plasmonic trap array for simultaneously compartmentalizing and sensitively detecting single-cell metabolites...
August 14, 2018: Analytical Chemistry
Chul-Suk Hong, Jae Hyung Park, Soonkoo Lee, Kun Yil Rhoo, Jong Tak Lee, Seung R Paik
A bioapplicable cargo delivery system requires the following characteristics of biocompatibility, in vivo stability, and selective cargo release at target sites. We introduce herein the microcapsules enclosed with a single-layered shell of gold nanoparticles (AuNPs) mutually connected by an amyloidogenic protein of α-synuclein (αS). The microcapsules were fabricated by producing oil(chloroform)-in-water Pickering emulsions of the αS-encapsulated AuNPs and subsequent molecular engagement of the outlying αS molecules, leading to formidable β-sheet formation in the presence of chloroform...
August 8, 2018: ACS Applied Materials & Interfaces
Steven Peiran Zhang, James Lata, Chuyi Chen, John Mai, Feng Guo, Zhenhua Tian, Liqiang Ren, Zhangming Mao, Po-Hsun Huang, Peng Li, Shujie Yang, Tony Jun Huang
For decades, scientists have pursued the goal of performing automated reactions in a compact fluid processor with minimal human intervention. Most advanced fluidic handling technologies (e.g., microfluidic chips and micro-well plates) lack fluid rewritability, and the associated benefits of multi-path routing and re-programmability, due to surface-adsorption-induced contamination on contacting structures. This limits their processing speed and the complexity of reaction test matrices. We present a contactless droplet transport and processing technique called digital acoustofluidics which dynamically manipulates droplets with volumes from 1 nL to 100 µL along any planar axis via acoustic-streaming-induced hydrodynamic traps, all in a contamination-free (lower than 10-10 % diffusion into the fluorinated carrier oil layer) and biocompatible (99...
July 26, 2018: Nature Communications
Guadalupe Montserrat Valdes Labrada, Mehdi Nemati
Waters contaminated with naphthenic acids (NAs) and associated tailings are one of the major environmental challenges associated with the processing of oil sands and production of heavy oil. In the current work biodegradation of linear and cyclic naphthenic acids, namely octanoic acid and 4-methyl-1-cyclohexane carboxylic acid (trans-4MCHCA), individually and in mixture were evaluated in microbial fuel cells (MFCs). In batch MFCs with single rod electrodes and freely suspended bacteria, biodegradation rate increased as NA initial concentration increased from 100 to 250 mg L-1 with no further improvement when a concentration of 500 mg L-1 was evaluated...
July 25, 2018: Bioprocess and Biosystems Engineering
Yolanda Schaerli
High-throughput screening of a DNA library expressed in a bacterial population for identifying potentially rare members displaying a property of interest is a crucial step for success in many experiments such as directed evolution of proteins and synthetic circuits and deep mutational scanning to identify gain- or loss-of-function mutants. Here, I describe a protocol for high-throughput screening of bacterial ( E. coli ) microcolonies in gel beads. Single cells are encapsulated into monodisperse water-in-oil emulsion droplets produced with a microfluidic device...
July 5, 2018: Bio-protocol
H X Cui, L P Guo, G P Zhao, R R Liu, Q H Li, M Q Zheng, J Wen
Intramuscular fat is important in improving meat quality; however, the lack of high-purity intramuscular preadipocyte (IMP) in vitro has severely limited the in-depth research on the mutual regulation of myocytes and adipocytes in chicken. In this study, we establish a new method by combining the mature adipocyte ceiling method and the transwell co-culture system. Mature intramuscular adipocyte (MIA) and muscle satellite cell (MSC) were obtained from digested pectoralis major, and MIAs were transformed into IMPs by dedifferentiation with ceiling culture...
July 11, 2018: Poultry Science
Richard C Laughlin, Rachel Madera, Yair Peres, Brian R Berquist, Lihua Wang, Sterling Buist, Yulia Burakova, Sreenath Palle, Chungwon J Chung, Max V Rasmussen, Erica Martel, David A Brake, John G Neilan, Sara D Lawhon, L Garry Adams, Jishu Shi, Sylvain Marcel
Classical Swine Fever Virus (CSFV) causes classical swine fever, a highly contagious hemorrhagic fever affecting both feral and domesticated pigs. Outbreaks of CSF in Europe, Asia, Africa, and South America had significant adverse impacts on animal health, food security and the pig industry. The disease is generally contained by prevention of exposure through import restrictions (e.g. banning import of live pigs and pork products), localized vaccination programs, and culling of infected or at-risk animals, often at very high cost...
July 11, 2018: Plant Biotechnology Journal
O Marisa Herrero, María S Villalba, Mariana P Lanfranconi, Héctor M Alvarez
The accumulation of triacylglycerols (TAG) is a common feature among actinobacteria belonging to Rhodococcus genus. Some rhodococcal species are able to produce significant amounts of those lipids from different single substrates, such as glucose, gluconate or hexadecane. In this study we analyzed the ability of different species to produce lipids from olive oil mill wastes (OMW), and the possibility to enhance lipid production by genetic engineering. OMW base medium prepared from alperujo, which exhibited high values of chemical oxygen demand (127,000 mg/l) and C/N ratio (508), supported good growth and TAG production by some rhodococci...
July 10, 2018: World Journal of Microbiology & Biotechnology
Yan Zheng, Guifang Feng, Yufei Sun, Shu Liu, Zifeng Pi, Fengrui Song, Zhiqiang Liu
Influencing the absorption of effective components in the intestines is one of the important compatibility mechanisms of the traditional Chinese medicine. Simulation of drug absorption through an in vitro intestinal epithelial cell line is an important method to study the interaction of drug compatibility and bioavailability of drugs. In this study, the compatibility mechanism of Ding-Zhi-Xiao-Wan (DZXW) was investigated by using the in vitro Caco-2 cell monolayers model. Decomposing the formula into single herb and drug-pair to clarify the compatibility mechanism was firstly used...
July 4, 2018: Journal of Pharmaceutical and Biomedical Analysis
Cinzia Formighieri, Anastasios Melis
Synechocystis (a cyanobacterium) was employed as an alternative host for the production of plant essential oil constituents. β-Phellandrene synthase (PHLS) genes from different plants, when expressed in Synechocystis, enabled synthesis of variable monoterpene hydrocarbon blends, converting Synechocystis into a cell factory that photosynthesized and released useful products. Monoterpene synthases are secondary metabolism enzymes that catalyze the generation of essential oil constituents in terrestrial plants...
July 4, 2018: Planta
X Z Liu, Y C Tao, X L Zhang, Z L Yu
Objective: To investigate the mechanism of cleft palate in mice induced by excessive all-trans retinoic acid (atRA). Methods: The pregnant mice were randomly divided into atRA-treated group ( n= 27) and control group ( n= 27). atRA-treated group was given by gavage a single dose of atRA (100 mg/kg) at 8: 00 AM on gestation day 10 (GD10) and the control group was given by gavage the isopyknic corn oil. At GD13-GD15, the fetal mice palate development was observed by HE staining. The mouse embryonic palatal mesenchymal cell proliferation was detected by 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry...
November 9, 2017: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
Kuangwen Hsieh, Helena C Zec, Liben Chen, Aniruddha M Kaushik, Kathleen E Mach, Joseph C Liao, Tza-Huei Wang
Simple, fast, and precise counting of viable bacteria is fundamental to a variety of microbiological applications such as food quality monitoring and clinical diagnosis. To this end, agar plating, microscopy, and emerging microfluidic devices for single bacteria detection have provided useful means for counting viable bacteria, but they also have their limitations ranging from complexity, time, and inaccuracy. We present herein our new method RAPiD (Resazurin-Amplified Picoarray Detection) for addressing this important problem...
July 17, 2018: Analytical Chemistry
Beatrice Polini, Maria Digiacomo, Sara Carpi, Simone Bertini, Francesca Gado, Giuseppe Saccomanni, Marco Macchia, Paola Nieri, Clementina Manera, Stefano Fogli
Although the anticancer properties of extra virgin olive oil (EVOO) extracts have been recognized, the role of single compounds in non-melanoma skin cancer is still unknown. The in vitro chemopreventive and anticancer action of EVOO extracts and oil-derived compounds in non-melanoma skin cancer models were evaluated on cutaneous squamous cell carcinoma cells and on immortalized human keratinocytes stimulated with epidermal growth factor. Preparation of EVOO extracts and isolation of single compounds was carried out by chromatographic methods...
June 28, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Agata Konarska
Lycium barbarum L. fruits, referred to as functional food, have long been used in traditional and folk herbal medicine due to their therapeutic properties. The fruit microstructure was analysed using light, scanning and transmission electron microscopes. The distribution of bioactive compounds in drupe tissues was assessed with histochemical and fluorescence assays. The analysis of the microstructure has shown that the fruit is covered by a skin with an amorphous cuticle and a layer of amorphous epicuticular waxes on the surface...
June 15, 2018: Protoplasma
Haitao Yu, Maaike Rump, Shantanu Maheshwari, Lei Bao, Xuehua Zhang
Surface droplets in the microscale are of great interest for their relevance in broad droplet-based technologies. Derived from the Ouzo effect, the solvent exchange process is a simple bottom-up approach to produce surface nano-/micro-droplets by the nucleation and growth mechanism. The oil oversaturation pulse is created as a good solvent (ethanol) for the oil displaced by a poor solvent (water) in the flow cell. In this work, we investigated the formation of surface droplets on a one-dimensional substrate (a single hydrophobic fiber with a diameter of 10 μm) in a flow...
July 11, 2018: Physical Chemistry Chemical Physics: PCCP
Catherine Madzak
Yarrowia lipolytica is an oleaginous saccharomycetous yeast with a long history of industrial use. It aroused interest several decades ago as host for heterologous protein production. Thanks to the development of numerous molecular and genetic tools, Y. lipolytica is now a recognized system for expressing heterologous genes and secreting the corresponding proteins of interest. As genomic and transcriptomic tools increased our basic knowledge on this yeast, we can now envision engineering its metabolic pathways for use as whole-cell factory in various bioconversion processes...
August 2018: Molecular Biotechnology
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