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CXCL1 melanocyte

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https://www.readbyqxmd.com/read/27732890/autophagy-deficient-melanocytes-display-a-senescence-associated-secretory-phenotype-that-includes-oxidized-lipid-mediators
#1
Chunya Ni, Marie-Sophie Narzt, Ionela-Mariana Nagelreiter, Cheng Feng Zhang, Lionel Larue, Heidemarie Rossiter, Johannes Grillari, Erwin Tschachler, Florian Gruber
Autophagy is a recycling program which allows cells to adapt to metabolic needs and to stress. Defects in autophagy can affect metabolism, aging, proteostasis and inflammation. Autophagy pathway genes, including autophagy related 7 (Atg7), have been associated with the regulation of skin pigmentation, and autophagy defects disturb the biogenesis and transport of melanosomes in melanocytes as well as transfer and processing of melanin into keratinocytes. We have previously shown that mice whose melanocytes or keratinocytes lack Atg7 (and thus autophagy) as a result of specific gene knockout still retained functioning melanosome synthesis and transfer, and displayed only moderate reduction of pigmentation...
December 2016: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/27377700/discrimination-of-dysplastic-nevi-from-common-melanocytic-nevi-by-cellular-and-molecular-criteria
#2
Hiroshi Mitsui, Felix Kiecker, Avner Shemer, Maria Vittoria Cannizzaro, Claire Q F Wang, Nicholas Gulati, Hanako Ohmatsu, Kejal R Shah, Patricia Gilleaudeau, Mary Sullivan-Whalen, Inna Cueto, Neil Scott McNutt, Mayte Suárez-Fariñas, James G Krueger
Dysplastic nevi (DNs), also known as Clark's nevi or atypical moles, are distinguished from common melanocytic nevi by variegation in pigmentation and clinical appearance, as well as differences in tissue patterning. However, cellular and molecular differences between DNs and common melanocytic nevi are not completely understood. Using cDNA microarray, quantitative RT-PCR, and immunohistochemistry, we molecularly characterized DNs and analyzed the difference between DNs and common melanocytic nevi. A total of 111 probesets (91 annotated genes, fold change > 2...
October 2016: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/25560632/melanoma-cells-influence-the-differentiation-pattern-of-human-epidermal-keratinocytes
#3
Ondřej Kodet, Lukáš Lacina, Eliška Krejčí, Barbora Dvořánková, Miloš Grim, Jiří Štork, Daniela Kodetová, Čestmír Vlček, Jana Šáchová, Michal Kolář, Hynek Strnad, Karel Smetana
BACKGROUND: Nodular melanoma is one of the most life threatening tumors with still poor therapeutic outcome. Similarly to other tumors, permissive microenvironment is essential for melanoma progression. Features of this microenvironment are arising from molecular crosstalk between the melanoma cells (MC) and the surrounding cell populations in the context of skin tissue. Here, we study the effect of melanoma cells on human primary keratinocytes (HPK). Presence of MC is as an important modulator of the tumor microenvironment and we compare it to the effect of nonmalignant lowly differentiated cells also originating from neural crest (NCSC)...
January 5, 2015: Molecular Cancer
https://www.readbyqxmd.com/read/23732752/il-17-and-tnf-synergistically-modulate-cytokine-expression-while-suppressing-melanogenesis-potential-relevance-to-psoriasis
#4
Claire Q F Wang, Yemsratch T Akalu, Mayte Suarez-Farinas, Juana Gonzalez, Hiroshi Mitsui, Michelle A Lowes, Seth J Orlow, Prashiela Manga, James G Krueger
Inflammation-associated pigmentation changes are extremely common, but the etiology underlying this clinical observation remains elusive. Particularly, it is unclear how the myriad of cytokines known to be involved in inflammatory skin processes affect epidermal melanocytes. We sought to determine how IL-17 and tumor necrosis factor (TNF) influence normal human melanocytes, as these two cytokines have been implicated in various skin diseases. IL-17 and TNF jointly stimulated broad inductions of cytokines, including melanoma mitogens CXCL1 and IL-8...
December 2013: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/20596077/ciglitazone-negatively-regulates-cxcl1-signaling-through-mitf-to-suppress-melanoma-growth
#5
T Botton, A Puissant, Y Cheli, T Tomic, S Giuliano, L Fajas, M Deckert, J-P Ortonne, C Bertolotto, S Tartare-Deckert, R Ballotti, S Rocchi
We have previously demonstrated that the thiazolidinedione ciglitazone inhibited, independently of PPARγ activation, melanoma cell growth. Further investigations now show that ciglitazone effects are mediated through the regulation of secreted factors. Q-PCR screening of several genes involved in melanoma biology reveals that ciglitazone inhibits expression of the CXCL1 chemokine gene. CXCL1 is overexpressed in melanoma and contributes to tumorigenicity. We show that ciglitazone induces a diminution of CXCL1 level in different human melanoma cell lines...
January 2011: Cell Death and Differentiation
https://www.readbyqxmd.com/read/18757499/inflamed-phenotype-of-the-mesenteric-microcirculation-of-melanocortin-type-3-receptor-null-mice-after-ischemia-reperfusion
#6
Giovanna Leoni, Hetal B Patel, André L F Sampaio, Felicity N E Gavins, Joanne F Murray, Paolo Grieco, Stephen J Getting, Mauro Perretti
The existence of anti-inflammatory circuits centered on melanocortin receptors (MCRs) has been supported by the inhibitory properties displayed by melanocortin peptides in models of inflammation and tissue injury. Here we addressed the pathophysiological effect that one MCR, MCR type 3 (MC3R), might have on vascular inflammation. After occlusion (35 min) and reopening of the superior mesenteric artery, MC3R-null mice displayed a higher degree of plasma extravasation (45 min postreperfusion) and cell adhesion and emigration (90 min postreperfusion)...
December 2008: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/16098041/endothelin-1-induces-cxcl1-and-cxcl8-secretion-in-human-melanoma-cells
#7
Catherine R Mangahas, Gelo V dela Cruz, George Friedman-Jiménez, Sumayah Jamal
The endothelin pathway plays a critical role in melanoma tumor progression by a variety of mechanisms that enhance tumor cell growth, invasion, and metastasis. Here, we investigate the effect of this pathway on CXC chemokine expression in human melanoma cells and melanocytes. As determined by ELISA, endothelin-1 (ET-1) induces CXCL1 and CXCL8 secretion in three human melanoma cell lines in a concentration-dependent fashion. These responses are mediated by the endothelin-B receptor and are sustained over a 40 h time course...
August 2005: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/12964041/evidence-of-involvement-of-cxc-chemokines-in-proliferation-of-cultivated-human-melanocytes
#8
Maja Mockenhaupt, Frank Peters, Ildiko Schwenk-Davoine, Yared Herouy, Ingrid Schraufstätter, Peter Elsner, Johannes Norgauer
The CXC-chemokines 1 and 8 (CXCL1 and CXCL8) are ligands for the G protein-coupled CXC-chemokine receptor 2 (CXCR2). Both chemokines and CXCR2 are components of a potent autocrine growth factor loop in human melanoma cells. Currently, expression and biological function of both chemokines in normal human melanocytes is poorly defined. Here we describe that cocktails of melanocyte growth factors consisting of basic fibroblast growth factor (bFGF), endothelin 1 (ET-1) and alpha-melanocyte-stimulating hormone (alpha-MSH) stimulated release of CXCL1 and CXCL8, but did not influence expression of CXCR2 in human melanocytes...
October 2003: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/12101257/role-of-cxcl1-in-tumorigenesis-of-melanoma
#9
REVIEW
Punita Dhawan, Ann Richmond
The CXC chemokine, CXCL1 (melanoma growth-stimulatory activity/growth-regulated protein alpha), plays a major role in inflammation, angiogenesis, tumorigenesis, and wound healing. Recently, chemokines have been extensively related to cellular transformation, tumor growth, homing, and metastasis. CXCL1 and its mouse homologue MIP-2 have been shown to be involved in the process of tumor formation. When chemokines such as CXCL1 and CXCL8 (IL-8) become disregulated so that they are chronically expressed, tissue damage, angiogenesis, and tumorigenesis can follow...
July 2002: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/12011258/melanoma-growth-stimulatory-activity-in-primary-malignant-melanoma-prognostic-significance
#10
Benton R Middleman, Michael Friedman, David H Lawson, Patricia B DeRose, Cynthia Cohen
Malignant melanoma (MM) cells do not require all exogenous growth factors of normal melanocytes. It is hypothesized that they make their own growth factors including melanoma growth stimulatory activity (MGSA). Cultured melanoma cells respond to MGSA with increased growth and angiogenesis suggesting a role for MGSA in MM proliferation, differentiation, and progression. We assessed the prognostic significance of MGSA expression in 37 primary MM immunostained for MGSA. Immunostains were graded for intensity (0-3+), percentage of cells immunostained, and location of immunostain (intraepidermal, junctional, or dermal)...
May 2002: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/11773061/a-novel-nf-kappa-b-inducing-kinase-mapk-signaling-pathway-up-regulates-nf-kappa-b-activity-in-melanoma-cells
#11
Punita Dhawan, Ann Richmond
Constitutive activation of NF-kappa B is an emerging hallmark of various types of tumors including breast, colon, pancreatic, ovarian, and melanoma. In melanoma cells, the basal expression of the CXC chemokine, CXCL1, is constitutively up-regulated. This up-regulation can be attributed in part to constitutive activation of NF-kappa B. Previous studies have shown an elevated basal I kappa B kinase (IKK) activity in Hs294T melanoma cells, which leads to an increased rate of I kappa B phosphorylation and degradation...
March 8, 2002: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/11719444/induction-of-melanoma-in-murine-macrophage-inflammatory-protein-2-transgenic-mice-heterozygous-for-inhibitor-of-kinase-alternate-reading-frame
#12
J Yang, J Luan, Y Yu, C Li, R A DePinho, L Chin, A Richmond
The molecular and genetic events that contribute to the genesis and progression of cutaneous malignant melanoma are poorly understood, attributable in large part to the different genetic alterations accompanying tumorigenesis. Inhibitor of kinase 4a (INK4a) is often inactivated in families with hereditary melanoma. Loss of INK4a/alternate reading frame (ARF) in mice is associated with increased incidence of other tumors such as lymphoma and fibrosarcoma. However, the incidence of melanoma in INK4a/ARF-deficient mice is very low...
November 15, 2001: Cancer Research
https://www.readbyqxmd.com/read/11406569/constitutive-ikappab-kinase-activity-correlates-with-nuclear-factor-kappab-activation-in-human-melanoma-cells
#13
J Yang, A Richmond
Constitutive IKK activity associated with increased IkappaBalpha phosphorylation and degradation contribute to the high level of endogenous nuclear factor-kappaB (NF-kappaB) activation in Hs294T melanoma cells as compared with RPE cells (R. L. Shattuck-Brandt and A. Richmond, Cancer Res., 57: 3032-3039, 1997; M. N. Devalaraja et al., Cancer Res., 59: 1372-1377, 1999). To determine whether this endogenous NF-kappaB activation was characteristic of melanoma, we examined the level of constitutive activation of NF-kappaB in a number of melanoma cell lines...
June 15, 2001: Cancer Research
https://www.readbyqxmd.com/read/11112786/a-role-for-poly-adp-ribose-polymerase-in-the-transcriptional-regulation-of-the-melanoma-growth-stimulatory-activity-cxcl1-gene-expression
#14
C Nirodi, S NagDas, S P Gygi, G Olson, R Aebersold, A Richmond
The melanoma growth stimulatory activity/growth-regulated protein, CXCL1, is constitutively expressed at high levels during inflammation and progression of melanocytes into malignant melanoma. It has been shown previously that CXCL1 overexpression in melanoma cells is due to increased transcription as well as stability of the CXCL1 message. The transcription of CXCL1 is regulated through several cis-acting elements including Sp1, NF-kappaB, HMGI(Y), and the immediate upstream region (IUR) element (nucleotides -94 to -78), which lies immediately upstream to the nuclear factor kappaB (NF-kappaB) element...
March 23, 2001: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/11062239/nuclear-factor-kappa-b-activation-by-the-cxc-chemokine-melanoma-growth-stimulatory-activity-growth-regulated-protein-involves-the-mekk1-p38-mitogen-activated-protein-kinase-pathway
#15
D Wang, A Richmond
Melanoma growth stimulatory activity/growth-regulated protein (MGSA/GRO), a CXC chemokine, plays an important role in inflammation, wound healing, growth regulation, angiogenesis, and tumorigenesis. Constitutive expression of MGSA/GROalpha in melanoma tumors is associated with constitutive nuclear factor (NF)-kappaB activity. We show here that either exogenous addition or continuous expression of MGSA/GROalpha in immortalized melanocytes enhances NF-kappaB activation, as well as mitogen-activated protein (MAP) kinase kinase kinase (MEKK) 1, MAP kinase kinase (MEK) 3/6, and p38 MAP kinase activation...
February 2, 2001: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/11030154/mgsa-gro-mediated-melanocyte-transformation-involves-induction-of-ras-expression
#16
D Wang, W Yang, J Du, M N Devalaraja, P Liang, K Matsumoto, K Tsubakimoto, T Endo, A Richmond
The MGSA/GRO protein is endogenously expressed in almost 70% of the melanoma cell lines and tumors, but not in normal melanocytes. We have previously demonstrated that over-expression of human MGSA/GROalpha, beta or gamma in immortalized murine melanocytes (melan-a cells) enables these cells to form tumors in SCID and nude mice. To examine the possibility that the MGSA/GRO effect on melanocyte transformation requires expression of other genes, differential display was performed. One of the mRNA's identified in the screen as overexpressed in MGSA/GRO transformed melan-a clones was the newly described M-Ras or R-Ras3 gene, a member of the Ras gene superfamily...
September 21, 2000: Oncogene
https://www.readbyqxmd.com/read/10816656/molecular-basis-of-the-alpha-msh-il-1-antagonism
#17
T Brzoska, D H Kalden, T Scholzen, T A Luger
The neuropeptide alpha-melanocyte stimulating hormone (alpha-MSH) is recognized as a potent mediator of immune and inflammatory reactions. Accordingly, alpha-MSH in vitro, as well as in vivo, antagonizes the proinflammatory activities of cytokines such as interleukin-1 (IL-1), IL-6, and tumor necrosis factor alpha (TNF alpha). Since the molecular basis of these antiinflammatory effects is not well known, the influence of alpha-MSH on IL-1 beta-induced chemokine production and transcription factor activation was investigated in human keratinocytes...
October 20, 1999: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/10647998/the-tumorigenic-and-angiogenic-effects-of-mgsa-gro-proteins-in-melanoma
#18
H Haghnegahdar, J Du, D Wang, R M Strieter, M D Burdick, L B Nanney, N Cardwell, J Luan, R Shattuck-Brandt, A Richmond
Continuous expression of the MGSA/GROalpha, beta, or gamma chemokine bestows tumor-forming capacity to the immortalized murine melanocyte cell line, melan-a. The mechanism for this transformation is unclear, although both autocrine and paracrine processes are possible because melan-a cells as well as endothelial cells express a low level of the receptor for this ligand. To further define the role of MGSA/GRO proteins in melanocyte transformation, two types of experiments were designed to neutralize the biological effects of MGSA/GRO in the transfected melan-a clones: (1) the effect of neutralizing antiserum to MGSA/GRO proteins on melan-a tumor growth was assessed; (2) the tumor-forming capacity of melan-a clones expressing ELR motif-mutated forms of MGSA/GRO with compromised receptor affinity was compared to the tumor-forming capacity of clones expressing wild-type MGSA/GRO...
January 2000: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/10380932/a-synthetic-peptide-inhibitor-for-alpha-chemokines-inhibits-the-tumour-growth-and-pulmonary-metastasis-of-human-melanoma-cells-in-nude-mice
#19
N Fujisawa, S Hayashi, E J Miller
Growth-related oncogene-alpha (GROalpha) was first described as an autocrine mitogen and growth factor for melanoma cells. More recent studies show that GROalpha, interleukin-8 (IL-8) and other members of the alpha-chemokine superfamily are also angiogenic. Therefore, we sought to determine if inhibitors of the alpha-chemokine receptor would be effective in inhibiting the tumour growth and pulmonary metastasis of human melanoma cells. We determined that melanocytes and 12 human melanoma cell lines produce both GROalpha and IL-8...
April 1999: Melanoma Research
https://www.readbyqxmd.com/read/10096573/elevated-constitutive-ikappab-kinase-activity-and-ikappab-alpha-phosphorylation-in-hs294t-melanoma-cells-lead-to-increased-basal-mgsa-gro-alpha-transcription
#20
M N Devalaraja, D Z Wang, D W Ballard, A Richmond
The basal transcription of the CXC chemokine, melanocyte growth stimulatory activity (MGSA)/growth-regulated protein (GRO)-alpha, is up-regulated in Hs294T melanoma cells compared with the normal retinal pigment epithelial (RPE) cells. Previous studies characterized a cytokine-inducible, functional nuclear factor (NF)-kappaB consensus element in the immediate 5' regulatory region of the MGSA/GRO-alpha gene at -78 bp. Although the cytokine-inducible mechanisms for transcription of this gene are fairly well delineated, the mechanisms involved in its basal up-regulation of transcription in Hs294T melanoma cells are poorly understood...
March 15, 1999: Cancer Research
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