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IFN-γ signaling

W Du, H Xu, X Mei, X Cao, L Gong, Y Wu, Y Li, D Yu, S Liu, Y Wang, W Li
Bacillus is widely used in the livestock industry. This study was designed to evaluate the effects of probiotic Bacillus amyloliquefaciens SC06 (Ba), originally isolated from soil, in piglets diet as an alternative to antibiotics (aureomycin), mainly on intestinal epithelial barrier and immune function. Ninety piglets were divided into three groups: G1 (containing 150 mg/kg aureomycin in the diet); G2 (containing 75 mg/kg aureomycin and 1×108 cfu/kg Ba in the diet); G3 (containing 2×108 cfu/kg Ba in the diet without any antibiotics)...
August 13, 2018: Beneficial Microbes
Meizhu Xiao, Ying Feng, Guangming Cao, Chongdong Liu, Zhenyu Zhang
OBJECTIVE: To evaluate the potential effects of recombinant mycobacterium tuberculosis heat shock protein 70-formyl peptide receptor 1 (MtHSP70-FPR1) fusion protein on human monocyte-derived dendritic cell (moDC) maturation; cytotoxic T lymphocyte (CTL) responses to cervical cancer (CC) cells; and the roles of the p38 MAPK, ERK, and JNK pathways in its transition. METHODS: Monocytes were positively selected with a MACS column with antiCD14 antibody-conjugated microbeads from umbilical cord blood...
August 8, 2018: Biochemical and Biophysical Research Communications
Qun Wang, Shuang Gao, Guo-Zhen Wu, Niao Yang, Xian-Peng Zu, Wen-Cai Li, Ning Xie, Rong-Rong Zhang, Chen-Wei Li, Zhen-Lin Hu, Wei-Dong Zhang
BACKGROUND: Inula helenium L. is an herb whose anti-inflammatory properties are attributed to its active components, the sesquiterpene lactones (SLs). Our previous study demonstrated that the total sesquiterpene lactones isolated from Inula helenium L. (TSL-IHL), consisting mainly of alantolactone (AL) and isoalantolactone (IAL), may have potential in the prevention and treatment of rheumatoid arthritis (RA). However, the effect of TSL-IHL on atopic dermatitis (AD) has not been studied yet...
July 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Aakanksha Jain, Ran Song, Edward K Wakeland, Chandrashekhar Pasare
Innate cytokines are critical drivers of priming and differentiation of naive CD4 T cells, but their functions in memory T cell response are largely undefined. Here we show that IL-1 acts as a licensing signal to permit effector cytokine production by pre-committed Th1 (IFN-γ), Th2 (IL-13, IL-4, and IL-5) and Th17 (IL-17A, IL-17F, and IL-22) lineage cells. This licensing function of IL-1 is conserved across effector CD4 T cells generated by diverse immunological insults. IL-1R signaling stabilizes cytokine transcripts to enable productive and rapid effector functions...
August 9, 2018: Nature Communications
Goo-Young Seo, Jr-Wen Shui, Daisuke Takahashi, Christina Song, Qingyang Wang, Kenneth Kim, Zbigniew Mikulski, Shilpi Chandra, Daniel A Giles, Sonja Zahner, Pyeung-Hyeun Kim, Hilde Cheroutre, Marco Colonna, Mitchell Kronenberg
Innate lymphoid cells (ILCs) are important regulators of early infection at mucosal barriers. ILCs are divided into three groups based on expression profiles, and are activated by cytokines and neuropeptides. Yet, it remains unknown if ILCs integrate other signals in providing protection. We show that signaling through herpes virus entry mediator (HVEM), a member of the tumor necrosis factor (TNF) receptor superfamily, in ILC3 is important for host defense against oral infection with the bacterial pathogen Yersinia enterocolitica...
August 8, 2018: Cell Host & Microbe
Yi Xie, Jun-Fang Yan, Jing-Yi Ma, Hong-Yan Li, Yan-Cheng Ye, Yan-Shan Zhang, Hong Zhang
High linear energy transfer radiation is known to deposit higher energy in tissues and cause greater toxicity compared to low-LET irradiation. Local immunosuppression is frequently observed after irradiation (IR). Dendritic cells (DCs) play important roles in the initiation and maintenance of the immune response. The dysfunction of DCs contributes to tumor evasion and growth. However, molecular mechanisms underlying the establishment of immune tolerance induced by heavy ion IR through this DC population are poorly understood...
November 1, 2017: Toxicology Research
Allison A Throm, Halima Moncrieffe, Amir B Orandi, Jeanette T Pingel, Theresa L Geurs, Hannah L Miller, Allyssa L Daugherty, Olga N Malkova, Daniel J Lovell, Susan D Thompson, Alexei A Grom, Megan A Cooper, Stephen T Oh, Anthony R French
Polyarticular juvenile idiopathic arthritis (JIA) is among the most challenging of the JIA subtypes to treat. Even with current biologic therapies, the disease remains difficult to control in a substantial subset of patients, highlighting the need for new therapies. The aim of this study was to use the high dimensionality afforded by mass cytometry with phospho-specific antibodies to delineate signaling abnormalities in immune cells from treatment-naive polyarticular JIA patients. Peripheral blood mononuclear cells were isolated from 17 treatment-naive polyarticular JIA patients, 10 of the patients after achieving clinical remission, and 19 healthy controls...
August 9, 2018: JCI Insight
Dai Xue, Zhongjian Gong, Fangyong Zhu, Yanjing Qiu, Xiaodan Li
BACKGROUND: In recent years, simvastatin has been demonstrated to be capable of inducing odontogenic differentiation in human dental pulp stem cells (DPSCs), which makes it a promising source for endodontic treatment in pulpitis. However, a comprehensive understanding of how simvastatin affects the behavior of DPSCs and its potential in pulpitis is still lacking. OBJECTIVES: In this study, we investigated the effects of simvastatin on the viability of inflamed DPSCs...
August 8, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Shan Li, Pengtao Gong, Lixin Tai, Xin Li, Xiaocen Wang, Chunyan Zhao, Xu Zhang, Zhengtao Yang, Ju Yang, Jianhua Li, Xichen Zhang
Neospora caninum is an obligate intracellular parasite, which causes significant economic losses in the cattle industry. However, the immune mechanism of the parasite-host interaction is not yet fully understood. Extracellular vesicles (EVs) have emerged as a ubiquitous mechanism by which almost all cells, especially immune and tumor cells, participate in intercellular communications. Although studies have indicated that EVs secreted by Toxoplasma gondii or Trypanosoma brucei promote exchanges of biological molecules important for the host-parasite interplay, however, EVs and their biological activities in N...
2018: Frontiers in Immunology
Kai Zhang, Ke Wang, Yue Huang, Xue Zhu, Minhao Xie, Jiaying Wang
Highly sensitive detection of proteins is essential to biomedical research as well as clinical diagnosis. However, usual detecting methods are complicated operating, time-costing and extensive label preparing. Here, we combined molecular beacon (MB) track mediated DNA walker and nicking enzyme assisted signal amplification method to develop a simple and ultrasensitive malachite green fluorescence biosensor for specific detection cytokine, interferon-γ (IFN-γ). The association of the IFN-γ with the corresponding aptamers of the dsDNA strands leads to free of DNA walker which trigged the generation of DNA track at the help of Nicking endonuclease (Nb...
November 1, 2018: Talanta
Qiang Liu, Yong-Li Zhang, Wei Hu, Shou-Ping Hu, Zhuo Zhang, Xue-Hui Cai, Xi-Jun He
The molecular repertoire of porcine alveolar macrophages (PAMs) is greatly affected by the microenvironment they are exposed to, and specifically by inflammatory cytokines, such as interferon gamma (IFN-γ) released by activated lymphocytes, and microbial products, such as lipopolysaccharide (LPS). In our previous study, we found that IFN-γ- and LPS-activated PAMs (M1) could inhibit porcine reproductive and respiratory syndrome virus (PRRSV) replication. In this study, comprehensive analysis of the expression profiles of the genes associated with the polarization of M0-type PAMs (resting) toward M1 phenotypes (activated by IFN-γ and LPS) led to the following main results: 1) 1551 and 1823 genes were upregulated or downregulated in M1-type PAMs, respectively, compared with M0-type PAMs; 2) Among these, genes encoding ASS1 and CRTAM were the most upregulated and downregulated, respectively; 3) Genes involved in cytokine-cytokine receptor interaction and the JAK/STAT signaling pathway were significantly upregulated, suggesting their critical role in cellular activation; and 4) Genes involved in antigen proteolysis and presentation (immunoproteasome subunits), and inhibition of virus replication (host restriction factors) were significantly upregulated, emphasizing the critical role of these cytokines in immunity...
August 4, 2018: Biochemical and Biophysical Research Communications
Luana Rossato, Suelen Silvana Dos Santos, Lucas Gonçalves Ferreira, Sandro Rogério de Almeida
Here we investigated the importance of Toll-like receptor 4 (TLR-4) in innate immune response to Sporothrix brasiliensis, a virulent fungus of Sporothrix spp. In vitro assays, using C57Bl/6 (wild type [WT]) bone marrow-derived macrophages (BMDMs), and TLR-4 knockout (TLR-4-/-) showed that the absence of TLR-4 resulted in impaired phagocytosis and lower levels of tumor necrosis factor α (TNF-α), interleukin (IL)-6, and nitric oxide. In vivo assays were also performed, and the mice (WT and TLR-4-/-) were intraperitoneally infected with S...
August 1, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Chen Liu, Juntao Chen, Baoqing Liu, Shunzong Yuan, Dawei Shou, Liang Wen, Xiaoying Wu, Weihua Gong
Since pro-inflammatory cytokine IL-18 and its receptor (IL-18R) are closely involved in regulating both adaptive and innate immune responses, it is conceivable that they might play an important role in organ transplantation. IL-18 can stimulate lymphocytes to produce the IFN-γ and regulate macrophage activity, thereby increasing the expression of proinflammatory cytokines including IL-1β, IL-6, CCL4 (macrophage inflammatory protein-1 β), CXCL2 (macrophage inflammatory protein-2), and CCL2 (monocyte chemotactic protein-1)...
August 3, 2018: European Cytokine Network
Qian-Lan Zhou, Tian-Yue Wang, Miao Li, Yun-Xiao Shang
Allergic asthma is a chronic inflammatory disease of the airways. T lymphocytes play an important role in the pathogenesis of asthma. The voltage-gated Kv1.3 potassium channel is a target for the preferential inhibition of TEM cells. In this study, we investigate the effects of PAP-1, a selective inhibitor of Kv1.3 channel, on the treatment of the neutrophilic asthma model. PAP-1 (40 mg/kg) was injected intraperitoneally into ovalbumin (OVA)-lipopolysaccharide (LPS)-challenged BALB/c mice. We found that the expression of the Kv1...
August 2, 2018: International Immunopharmacology
Yong Shen, Saisai Wang, Fangfang Sun, Gang Zheng, Tingting Wu, Yushen Du, Suzhan Zhang, Jing Qian, Ren Sun
Gamma interferon (IFN-γ) is known to negatively regulate murine gammaherpesvirus-68 (MHV-68 or γHV-68) replication. This process involves the suppression of the viral gene replication and transcription activator (RTA) promoter, as well as activation of signal transducers and activators of transcription (STAT1). Notably, this effect is gradually attenuated during MHV-68 infection of bone marrow-derived macrophages (BMMs), which raised the possibility that the virus may utilize a mechanism that counteracts the antiviral effect of IFN-γ...
August 3, 2018: PLoS Pathogens
Lehn K Weaver, Niansheng Chu, Edward M Behrens
OBJECTIVE: Macrophage Activation Syndrome (MAS) is a life-threatening cytokine storm syndrome that occurs in patients with underlying rheumatologic diseases. Preclinical and clinical data suggest that interferon (IFN)γ is pathogenic in MAS, yet how IFNγ causes disease remains unknown. In this manuscript, we sought to determine whether IFNγ-dependent signals synergize with systemic innate immune responses to drive cytokine storm in a murine model of MAS. METHODS: IFNγ-deficient mice were treated with five doses of a Toll-like receptor (TLR)9 agonist (CpG1826), IFNγ, or a combination of the two stimuli over the course of ten days...
August 2, 2018: Arthritis & Rheumatology
Adam S Arterbery, Jie Yao, Andrew Ling, Yaron Avitzur, Mercedes Martinez, Steven Lobritto, Yanhong Deng, Gan Geliang, Sameet Mehta, Guilin Wang, James Knight, Udeme D Ekong
De novo autoimmune hepatitis (DAIH) is an important cause of late allograft dysfunction following liver transplantation, but its cause and underlying pathogenesis remains unclear. We sought to identify specific innate and adaptive immune mechanisms driving the pro-inflammatory cytokine secreting regulatory T cell (Treg) phenotype in DAIH and determine if modulation of these pathways could resolve the inflammatory milieu observed in the livers of patients with DAIH. Here, we demonstrate toll-like receptors (TLRs) 2- and 4-mediated inflammasome activation in CD14++ monocytes, a finding that is key to maintaining dysfunctional Tregs in patients with DAIH...
2018: Frontiers in Immunology
Long Yi, Luxi Chen, Xiaofeng Guo, Ting Lu, Haixia Wang, Xiaotian Ji, Jianying Zhang, Yulin Ren, Pan Pan, A Douglas Kinghorn, Xiaohua Huang, Li-Shu Wang, Zhijin Fan, Michael A Caligiuri, Jianhua Yu
Natural products and their derivatives have long been used as pharmacological agents in the fight against cancer. Human natural killer (NK) cells are critical in our immune system in that they are capable of destroying tumor cells directly. However, there are few reports that elucidate the role of natural products in activating NK cells. In this study, we discovered that a synthetic disaccharide derivative of diphyllin, 4- O -{[2'',3'',4''-tri- O -acetyl-α-D-arabinopyranosyl-(1''→4')]-2',3'-di- O -acetyl-α-L-rhamnopyranosyl}diphyllin (TAARD), can alone stimulate interferon (IFN)-γ secretion in primary human NK cells and the NKL cell line...
2018: Frontiers in Immunology
Ji-Hyun Lee, Hyeon-Hui Ki, Dae-Ki Kim, Young-Mi Lee
Atopic dermatitis (AD) is a common chronic, recurring, inflammatory skin disease. A number of researchers have been seeking safe AD therapies for a long time. Triticum aestivum sprouts (TAEE), known as wheatgrass, are one of the most widely used health foods worldwide. They show numerous beneficial effects, including anticancer, anti‑inflammatory, anti‑oxidant, anti‑obesity, anti‑colitis and anti‑allergy effects; however, their effect on AD is unknown. In the present study, the anti‑AD effects of a 70% ethanol extract of TAEE were investigated in 2,4‑dinitrochlorobenzene (DNCB)‑treated mice with AD‑like skin lesions and in tumor necrosis factor (TNF)‑α‑ and interferon (IFN)‑γ‑stimulated human keratinocytes (HaCaT cells)...
July 31, 2018: Molecular Medicine Reports
Amit Kishore, Britt-Sabina Petersen, Marcel Nutsua, Joachim Müller-Quernheim, Andre Franke, Annegret Fischer, Stefan Schreiber, Martin Petrek
Genome-wide and candidate gene studies for pulmonary sarcoidosis have highlighted several candidate variants among different populations. However, the genetic basis of functional rare variants in sarcoidosis still needs to be explored. To identify functional rare variants in sarcoidosis, we sequenced exomes of 22 sarcoidosis cases from six families. Variants were prioritized using linkage and high-penetrance approaches, and filtered to identify novel and rare variants. Functional networking and pathway analysis of identified variants was performed using gene ontology based gene-phenotype, gene-gene, and protein-protein interactions...
July 27, 2018: Human Genetics
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