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Zhenhua Zhao, Liling Xia, Xin Jiang, Yanzheng Gao
The effects of different water-saving modes on PAHs residue and risk, field environment conditions and enzyme activities in paddy field were investigated in a field experiment plot in Laoyaba, Nanjing, China. Results showed that (1) water-saving treatment affected greatly the ΣPAHs in water and soil. The order of ΣPAHs residue in surface water and groundwater in farmland is as follows: dry fields<water-saving paddy field<flooding irrigation paddy field. The ΣPAHs in water during rice tillering stage were obviously higher than that in rice booting stage and milky stage, and the percentage of high-ring PAHs gradually reduced in water...
October 17, 2017: Science of the Total Environment
Jiayang Liu, Zhixin Wang, Hongyan Li, Changwei Hu, Paul Raymer, Qingguo Huang
The effect of solid state fermentation of peanut shell to produce beneficial laccase and on its dye adsorption performance was evaluated. The resulting residues from solid fermentation were tested as sorbents (designated as SFs) in comparison to the raw peanut shell (RPS) for their ability to remove crystal violet from water. The fermentation process reduced the adsorption capacity (qm) of SF by about 50%, and changed the sorptive behavior when compared to the RPS. The Langmuir model was more suitable for fitting adsorption by SFs...
October 6, 2017: Bioresource Technology
Baozhong Chai, Yunqian Qiao, He Wang, Xiaoming Zhang, Jiao Wang, Choushi Wang, Ping Zhou, Xiangdong Chen
Pyomelanin is the major constituent of pigment in melanogenic Aeromonas strains of bacteria. However, eumelanin, synthesized from tyrosine via L-DOPA and polyphenol oxidases (PPOs), may also be present in this genus since L-DOPA is frequently detected in culture fluids of several species. To address this question, we used a deletion mutant of Aeromonas media strain WS, in which pyomelanin synthesis is completely blocked under normal culture conditions. When tyrosine was supplied to the medium, we observed residual melanin accumulation, which we interpret as evidence for existence of the DOPA-melanin pathway...
2017: Frontiers in Microbiology
Roberto Orellana, Gina Chaput, Lye Meng Markillie, Hugh Mitchell, Matt Gaffrey, Galya Orr, Kristen M DeAngelis
The production of lignocellulosic-derived biofuels is a highly promising source of alternative energy, but it has been constrained by the lack of a microbial platform capable to efficiently degrade this recalcitrant material and cope with by-products that can be toxic to cells. Species that naturally grow in environments where carbon is mainly available as lignin are promising for finding new ways of removing the lignin that protects cellulose for improved conversion of lignin to fuel precursors. Enterobacter lignolyticus SCF1 is a facultative anaerobic Gammaproteobacteria isolated from tropical rain forest soil collected in El Yunque forest, Puerto Rico under anoxic growth conditions with lignin as sole carbon source...
2017: PloS One
Zepeng Kang, Kailong Jiao, Jin Cheng, Ruiyun Peng, Shuqiang Jiao, Zongqian Hu
A novel three-dimensional (3D) carbon composite of PANI1600@CNTs with rhizobium-like structure is prepared by in-situ polymerization of aniline monomers around and along the functionalized carbon nanotubes (CNTs) and then carbonized at 1600°C for enzymatic biofuel cells (EBFCs). The SEM and TEM images clearly show that the carbonized PANI grew seamlessly on the surface of CNTs and presented the rhizobium-like structure. The carbonized PANI acts like conductive "glue" and connects the adjacent tubes together, which can assemble the CNTs into a 3D network...
October 7, 2017: Biosensors & Bioelectronics
Timothy D Leathers, Joseph O Rich, Melinda S Nunnally, Amber M Anderson
OBJECTIVE: To test the inactivation of the antibiotic, virginiamycin, by laccase-induced culture supernatants of Aureobasidium pullulans. RESULTS: Fourteen strains of A. pullulans from phylogenetic clade 7 were tested for laccase production. Three laccase-producing strains from this group and three previously identified strains from clade 5 were compared for inactivation of virginiamycin. Laccase-induced culture supernatants from clade 7 strains were more effective at inactivation of virginiamycin, particularly at 50 °C...
October 16, 2017: Biotechnology Letters
Yuancheng Qi, Chao Liu, Xiankai Sun, Liyou Qiu, Jinwen Shen
Pleurotus ostreatus laccase gene poxc is transcriptionally induced by copper, and several putative MREs have been found and confirmed in its promoter region. However, the related transcription factors mediating copper response via MREs have not been reported. To isolate MRE binding proteins, we carried out yeast one-hybrid screens. Reporter genes containing two trimers of the cMRE2 and cMRE3 element were prepared and introduced into a yeast strain. The yeast was transformed with library cDNA that represents RNA isolated from CuSO4-treated fungi of P...
November 2017: Fungal Biology
Yichao Rui, Deirdre B Gleeson, Daniel V Murphy, Frances C Hoyle
One of the greatest contemporary challenges in terrestrial ecology is to determine the impact of climate change on the world's ecosystems. Here we investigated how wetting patterns (frequency and intensity) and nutrient additions altered microbial biomass and CO2-C loss from a semi-arid soil. South-western Australia is predicted to experience declining annual rainfall but increased frequency of summer rainfall events when soil is fallow. Agricultural soils (0-10 cm at 10 °C or 25 °C) received the same total amount of water (15 mL over 30 days) applied at different frequency; with either nil or added nitrogen and phosphorus...
October 12, 2017: Scientific Reports
Sara Callejón, Ramón Sendra, Sergi Ferrer, Isabel Pardo
Biogenic amines degradation by bacterial laccases is little known, so we have cloned and heterologously expressed, in E. coli, a new laccase from Pediococcus acidilactici CECT 5930 (Lpa5930), a lactic acid bacterium commonly found in foods able to degrade tyramine. The recombinant enzyme has been characterized by physical and biochemical assays. Here we report the optimization of expression and purification procedures of this laccase. DNA encoding sequence of laccase from P. acidilactici was amplified by PCR and cloned into the expression plasmid pET28a for induction by isopropyl-β-D-thiogalactoipyranoside...
2017: PloS One
Jakub Zdarta, Katarzyna Antecka, Robert Frankowski, Agnieszka Zgoła-Grześkowiak, Hermann Ehrlich, Teofil Jesionowski
Due to the rapid growth in quantities of phenolic compounds in wastewater, the development of efficient and environmentally friendly methods for their removal becomes a necessity. Thus, in a presented work, for the first time, a novel material, Hippospongia communis spongin-based scaffold, was used as a biopolymeric support for the immobilization of laccase from Trametes versicolor. The resulting biocatalytic systems were used for the biodegradation of three bisphenols: bisphenol A (BPA), bisphenol F (BPF) and bioremoval-resistant bisphenol S (BPS)...
October 6, 2017: Science of the Total Environment
Luong N Nguyen, Faisal I Hai, James A McDonald, Stuart J Khan, William E Price, Long D Nghiem
This study demonstrates continuous enantiomeric inversion and further biotransformation of chiral profens including ibuprofen, naproxen and ketoprofen by an enzymatic membrane bioreactor (EMBR) dosed with laccase. The EMBR showed non-enantioselective transformations, with high and consistent transformation of both (R)- and (S)-ibuprofen (93 ± 6%, n = 10), but lower removals of both enantiomers of naproxen (46 ± 16%, n = 10) and ketoprofen (48 ± 17%, n = 10). Enantiomeric analysis revealed a bidirectional but uneven inversion of the profens, for example 14% inversion of (R)- to (S)- compared to 4% from (S)- to (R)-naproxen...
October 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
Chiara Di Bari, Nicolas Mano, Sergey Shleev, Marcos Pita, Antonio L De Lacey
An infrared spectroelectrochemical study of Trametes hirsuta laccase and Magnaporthe oryzae bilirubin oxidase has been performed using azide, an inhibitor of multicopper oxidases, as an active infrared probe incorporated into the T2/T3 copper cluster of the enzymes. The redox potential-controlled measurements indicate that N3(-) stretching IR bands of azide ion bound to the T2/T3 cluster are only detected for the oxidized enzymes, confirming that azide only binds to Cu(2+). Moreover, the process of binding/dissociation of azide ion is shown to be reversible...
October 3, 2017: Journal of Biological Inorganic Chemistry: JBIC
Muhammad B Asif, Faisal I Hai, Jinguo Kang, Jason P van de Merwe, Frederic D L Leusch, William E Price, Long D Nghiem
Laccase-catalyzed degradation of a broad spectrum of trace organic contaminants (TrOCs) by a membrane distillation (MD)-enzymatic membrane bioreactor (EMBR) was investigated. The MD component effectively retained TrOCs (94-99%) in the EMBR, facilitating their continuous biocatalytic degradation. Notably, the extent of TrOC degradation was strongly influenced by their molecular properties. A significant degradation (above 90%) of TrOCs containing strong electron donating functional groups (e.g., hydroxyl and amine groups) was achieved, while a moderate removal was observed for TrOCs containing electron withdrawing functional groups (e...
September 20, 2017: Bioresource Technology
Chunliang Xie, Wenbing Gong, Zuohua Zhu, Li Yan, Zhenxiu Hu, Yuande Peng
Blue light is an important environmental factor which could induce mushroom primordium differentiation and fruiting body development. However, the mechanisms of Pleurotus eryngii primordium differentiation and development induced by blue light are still unclear. The CAZymes (carbohydrate-active enzymes) play important roles in degradation of renewable lignocelluloses to provide carbohydrates for fungal growth, development and reproduction. In the present research, the expression profiles of genes were measured by comparison between the Pleurotus eryngii at primordium differentiated into fruiting body stage after blue light stimulation and dark using high-throughput sequencing approach...
September 29, 2017: Genomics
Valentina Wyman, Josefa Henríquez, Carolyn Palma, Andrea Carvajal
Lignocellulosic wastes are generally pre-treated to facilitate the hydrolysis stage during the anaerobic digestion process. A process consisting of solid state fermentation carried out by white rot fungi and anaerobic digestion was evaluated on corn stover to produce ligninolytic enzymes and biogas. The enzyme production was quantified every 3d for a month at 30°C, and three fungal strains and two particle sizes of waste were compared. Of the main outcomes, Pleurotus eryngii produced the highest laccase enzyme activity compared with Pleurotus ostreatus and Trametes versicolor...
September 8, 2017: Bioresource Technology
Raheleh Ravanfar, Talita A Comunian, Robin Dando, Alireza Abbaspourrad
A new technique is presented to optimize the formulation of microcapsules loaded with labile compounds. Fish oil was loaded into the microcapsule core and protected with a shell composed of whey protein microgel/beet pectin complexes. The microcapsules were formed using two different methods: microfluidics and homogenization. The microcapsules were further classified into three sub-groups. The first group was the microcapsules cross-linked with laccase (MCL), the second group was the microcapsules cross-linked with divalent cationic CaCl2 salt (MCS), and the third group consisted of control microcapsules (CM), with no cross-linking...
February 15, 2018: Food Chemistry
O Saoudi, N Ghaouar, S Ben Salah, T Othman
In this work, we are interested in the denaturation process of a laccase from Tramates versicolor via the determination of the refractive index, the refractive index increment and the specific volume in various media. The measurements were carried out using an Abbe refractometer. We have shown that the refractive index increment values obtained from the slope of the variation of the refractive index vs. Concentration are outside the range refractive index increments of proteins. To correct the results, we have followed the theoretical predictions based on the knowledge of the protein refractive index from its amino acids composition...
September 2017: Biochemistry and Biophysics Reports
Rim Mtibaà, Laura de Eugenio, Bouthaina Ghariani, Ibtihel Louati, Lasaad Belbahri, Moncef Nasri, Tahar Mechichi
A novel fungal laccase produced by the ascomycete Chaetomium sp. isolated from arid soil was purified and characterized and its ability to remove dyes was determined. Extracellular laccase was purified 15-fold from the crude culture to homogeneity with an overall yield of 50% using ultrafiltration and anion-exchange chromatography. The purified enzyme was found to be a monomeric protein with a molecular mass of 68 kDa, estimated by SDS-PAGE, and with an isoelectric point of 5.5. The optimal temperature and pH value for laccase activity toward 2,6-DMP were 60 °C and 3...
October 2017: 3 Biotech
Prakram Singh Chauhan, Bindi Goradia, Arunika Saxena
Laccases (benzenediol: oxygen oxidoreductase, EC are multi-copper enzymes which catalyze the oxidation of a wide range of phenolic and non-phenolic aromatic compounds in the presence or absence of a mediator. Till date, laccases have mostly been isolated from fungi and plants, whereas laccase from bacteria has not been well studied. Bacterial laccases have several unique properties that are not characteristics of fungal laccases such as stability at high temperature and high pH. Bacteria produce these enzymes either extracellularly or intracellularly and their activity is in a wide range of temperature and pH...
October 2017: 3 Biotech
Rosa María Damián-Robles, Agustín Jaime Castro-Montoya, Jaime Saucedo-Luna, Ma Soledad Vázquez-Garcidueñas, Marina Arredondo-Santoyo, Gerardo Vázquez-Marrufo
Fungal strains identified by phylogenetic analysis of the ITS rDNA region as Trametes versicolor (CMU-TA01), Irpex lacteus (CMU-84/13), and Phlebiopsis sp. (CMU-47/13) are able to grow on and bleach kraft pulp (KP) in a simple solid-state fermentation (SSF) assay conducted in Petri dishes. Kappa number reductions obtained with Phlebiopsis sp. (48.3%), T. versicolor (43%), and I. lacteus (39.3%), evidence their capability for lignin breakdown. Scanning electron microscopy images of KP fibers from SSF assays demonstrated increased roughness and striation, evidencing significant cell wall modification...
October 2017: 3 Biotech
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