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Hui Li, Jingwei Hou, Linlin Duan, Chao Ji, Yatao Zhang, Vicki Chen
High efficient enzyme immobilization on carbon based conductive supports could provide wide applications in energy and environmental science. Here, we synthesized a 3D flower-like structured self-assembly hybrid nanocomposite with copper phosphate, laccase, graphite oxide (GO) and carbon nanotubes (CNTs) via a facile one-pot strategy under mild conditions. The prepared nanocomposite exhibited very high enzyme loading and improved laccase activity. During the formation of the nanocomposite, the copper phosphate-laccase petals were interwined by CNTs, and GO nanosheets were further coated on the petal surface...
May 11, 2017: Journal of Hazardous Materials
Juan Wan, Xiaowen Sun, Cheng Liu, Mengjun Tang, Lin Li, Hong Ni
A triplicate volcanic rock matrix-Bacillus thuringiensis-laccase WlacD (VRMs-Bt-WlacD) dye decolorization system was developed. WlacD was displayed on the B. thuringiensis MB174 cell surface to prepare a whole-cell laccase biocatalyst by using two repeat N-terminal domains of autolysin Mbg (Mbgn)2 as the anchoring motif. Immunofluorescence microscopic assays confirmed that the fusion protein (Mbgn)2-WlacD was anchored on the surface of the recombinant B. thuringiensis MB174. After optimization by a single factor test, L 9(3(4))-orthogonal test, Plackett-Burman test, steepest ascent method, and Box-Behnken response surface methodology, the whole-cell specific laccase activity of B...
June 2017: World Journal of Microbiology & Biotechnology
Vishal V Chandanshive, Niraj R Rane, Asif S Tamboli, Avinash R Gholave, Rahul V Khandare, Sanjay P Govindwar
Field treatment of textile industry effluent was carried out in constructed drenches (91.4m×1.2m×0.6m; 65.8m(3)) planted independently with Typha angustifolia, Paspalum scrobiculatum and their co-plantation (consortium-TP). The in situ treatment of effluent by T. angustifolia, P. scrobiculatum and consortium-TP was found to decrease ADMI color value by 62, 59 and 76%, COD by 65, 63 and 70%, BOD by 68, 63 and 75%, TDS by 45, 39 and 57%, and TSS by 35, 31 and 47%, respectively within 96h. Heavy metals such as arsenic, cadmium, chromium and lead were also removed up to 28-77% after phytoremediation...
May 12, 2017: Journal of Hazardous Materials
Jinhui Wang, Juanjuan Feng, Weitao Jia, Pengxiang Fan, Hexigeduleng Bao, Shizhong Li, Yinxin Li
Laccase is a key enzyme in plant lignin biosynthesis as it catalyzes the final step of monolignols polymerization. Sweet sorghum [Sorghum bicolor (L.) Moench] is considered as an ideal feedstock for ethanol production, but lignin greatly limits the production efficiency. No comprehensive analysis on laccase has ever been conducted in S. bicolor, although it appears as the most promising target for engineering lignocellulosic feedstock. The aim of our work is to systematically characterize S. bicolor laccase gene family and to identify the lignin-specific candidates...
2017: Frontiers in Plant Science
Alida Musatti, Elena Ficara, Chiara Mapelli, Cecilia Sambusiti, Manuela Rollini
Studies were performed on the use of the solid fraction of digestate (D) for the production of lignocellulolytic enzymes (endo- and exo-glucanase, xylanase, β-glucosidase and laccase) by fungi, in comparison with wheat straw (benchmark) (W). To date, this is the first report on the use of such an inexpensive substrate in a liquid environment. Submerged instead of solid state fermentation was applied to overcome pH inhibition and increase surface accessibility. A total of 21 fungal strains were tested: the most performing ones were Irpex lacteus DSM1183 for both β-glucosidase (52 IU/g with D, + 400% compared to W) and endo-glucanase (236 IU/g with D, + 470% compared to W), Schizophyllum commune CBS30132 for xylanase (715 IU/g with W, + 145% compared to D) and Pleurotus ostreatus ATCC96997 for laccase (124 IU/g with D, +230% compared to D)...
May 15, 2017: Journal of Environmental Management
Nazri Nayan, Anton S M Sonnenberg, Wouter H Hendriks, John W Cone
AIM: The present study evaluated differences between two strains of Ceriporiopsis subvermispora on improving the nutritive value and in vitro degradability of wheat straw. METHODS AND RESULTS: Wheat straw was treated with the fungi for 7 weeks. Weekly samples were analyzed for ergosterol content, in vitro gas production (IVGP), chemical composition and lignin degrading enzyme activity. Ergosterol data showed CS1 to have a faster initial growth than CS2 and reaching a stationary phase after 3 weeks...
May 18, 2017: Journal of Applied Microbiology
Ramón Alberto Batista-García, Vaidyanathan Vinoth Kumar, Arielle Ariste, Omar Eduardo Tovar-Herrera, Olivier Savary, Heidy Peidro-Guzmán, Deborah González-Abradelo, Stephen A Jackson, Alan D W Dobson, María Del Rayo Sánchez-Carbente, Jorge Luis Folch-Mallol, Roland Leduc, Hubert Cabana
A number of fungal strains belonging to the ascomycota, basidiomycota and zygomycota genera were subjected to an in vitro screening regime to assess their ligninolytic activity potential, with a view to their potential use in mycoremediation-based strategies to remove phenolic compounds and polycyclic aromatic hydrocarbons (PAHs) from industrial wastewaters. All six basidiomycetes completely decolorized remazol brilliant blue R (RBBR), while also testing positive in both the guaiacol and gallic acid tests indicating good levels of lignolytic activity...
May 9, 2017: Journal of Environmental Management
Xi Cheng, Chongchong Yan, Jinyun Zhang, Chenhui Ma, Shumei Li, Qing Jin, Nan Zhang, Yunpeng Cao, Yi Lin, Yongping Cai
The high-throughput sequencing of pear "Dangshan Su" × "Yali" (whose fruits lignin and stone cell content are high and quality is poor) and pear "Dangshan Su" × "Wonhwang" (whose fruits with low content of lignin and stone cell and the quality are better ) found that the expressions of these two miRNAs (pyr-1809 and pyr-novel-miR-144-3p) were significantly different; their corresponding target genes encode two kinds of laccase (Pbr018935.1 and Pbr003857.1). qRT-PCR results showed that these two enzymes are involved in the formation of lignin and stone cells and the existence of these two miRNAs has a negative effect on them...
2017: BioMed Research International
Sakihito Kitajima, Taiki Imamura, Junpei Iibushi, Makoto Ikenaga, Yoichi Tachibana, Nobuyuki Andoh, Hiroshi Oyabu, Kiyoo Hirooka, Takashi Shiina, Yoko Ishizaki
Lacquer tree sap, a raw material of traditional paints in East Asia, is hardened through laccase-catalyzed oxidation and the following polymerization of phenolic compound urushiol. In the sap's water-insoluble fraction, we found two plantacyanins and a ferritin 2 domain-containing protein (TvFe2D, a homolog of Arabidopsis AT1G47980 and AT3G62730). The recombinant TvFe2D protein suppressed the accumulation of laccase-catalyzed oxidation products of a model substrate syringaldazine without decreasing oxygen consumption, the second substrate of laccase...
June 2017: Bioscience, Biotechnology, and Biochemistry
Liang Shi, Dongdong Chen, Chao Xu, Ang Ren, Hanshou Yu, Mingwen Zhao
Flammulina velutipes is a well-known edible mushroom cultivated all over the world. However, because of the low transformation frequency, the expensive instruments required, and the complicated, time-consuming procedures necessary, there is insufficient genetic research on F. velutipes. In this study, we report a liposome-mediated transformation (LMT) system for the genetic transformation of F. velutipes. Using the LMT system, we obtained 82 ± 4 stable F. velutipes transformants per 10(5) protoplasts, which is a clear increase in transformation frequency compared to the other methods used...
May 2, 2017: Journal of General and Applied Microbiology
Yingli Cai, Yuhua Gong, Wei Liu, Yue Hu, Lianfu Chen, Lianlian Yan, Yan Zhou, Yinbing Bian
Lentinula edodes has the potential to degrade woody and nonwoody lignocellulosic biomass. However, the mechanism of lignocellulose degradation by L. edodes is unclear. The aim of this work is to explore the profiling of soluble secreted proteins involved in lignocellulose degradation in L. edodes. For that, we compared the secretomes of L. edodes grown on microcrystalline cellulose, cellulose with lignosulfonate and glucose. Based on nanoliquid chromatography coupled with tandem mass spectrometry of whole-protein hydrolysate, 230 proteins were identified...
May 5, 2017: Journal of Proteomics
Seyda Korkut, Muhammet Samet Kilic, Timur Sanal, Baki Hazer
This study describes construction of an enzymatic fuel cell comprised of poly(caprolactone-g-ethylene glycol) coated novel glucose oxidase anode and laccase cathode. Rationally designed poly(caprolactone-g-ethylene glycol) containing various poly(ethylene glycol) percentages ranging between 2.67 and 15.04% were synthesized chemically and tested separately for operation of the fuel cell system to achieve the best energy generation. The maximum power density was found to be 80.55μWcm(-2) at 0.91V (vs. Ag/AgCl) in pH5, 100mM citrate buffer (20°C) by the addition of 30mM of glucose from the electrodes coated with 11...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Roberto Sánchez-Sánchez, Alejandra Romero-Montero, Carmina Montiel, Yaaziel Melgarejo-Ramírez, Carmina Sánchez-Ortega, Haydée Lugo-Martínez, Beatriz Cabello-Arista, Roeb García-Arrazola, Cristina Velasquillo, Miquel Gimeno
The poly(gallic acid), produced by laccase-mediated oxidation of gallic acid in aqueous media (pH5.5) to attain a novel material with well-defined molecular structure and high water solubility (500mg/mL at 25°C), has been investigated to understand its potential biological activities. In this regard, a biomedical approach based on cytoprotective effect on human fibroblast cells exposed to UV-irradiation in the presence of the polymer has been demonstrated. The results also shows that 200μg/mL of poly(gallic acid) inhibits the growth and migration of dermal fibroblasts and cancer cell lines without affecting cell viability...
July 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Julian Ihssen, Dagmara Jankowska, Thomas Ramsauer, Renate Reiss, Ronny Luchsinger, Luzia Wiesli, Mark Schubert, Linda Thöny-Meyer, Greta Faccio
Laccases and laccase-like multi-copper oxidases (LMCOs) are versatile and robust biocatalysts applied in a variety of oxidative processes, and various studies have attempted to improve their catalytic activity. Here we report the engineering of a bacterial LMCO for enhanced oxidation of the lignin-related compound guaiacol by a combination of structure-guided mutagenesis and DNA shuffling. Mutant L9 showed a 1.39 mM Km for guaiacol and a 2.5-fold increase in turnover rate (kcat/Km = 2.85·104 M-1s-1).
May 8, 2017: Protein Engineering, Design & Selection: PEDS
Lili Shi, Siuming Chan, Chaozheng Li, Shuang Zhang
Phenoloxidases (POs) are a family of enzymes including tyrosinases, catecholases and laccases, which play an important role in immune defences of various invertebrates. Whether or not laccase exists in shrimp and its function is still poorly understood. In this study, a laccase (LvLac) was cloned and identified from Litopenaeus vannamei for the first time. The full length of LvLac is 3406 bp, including a 2034 bp open reading frame (ORF) coding for a putative protein of 677 amino acids with a signal peptide of 33 aa...
May 3, 2017: Fish & Shellfish Immunology
Amit Kumar, Deepti Singh, Krishna K Sharma, Sakshi Arora, Amarjeet K Singh, Sarvajeet S Gill, Barkha Singhal
Basidiomycetous fungi, Ganoderma lucidum MDU-7 and Ganoderma sp. kk-02 secreted multiple laccase isozymes under diverse growth condition. Aromatic compounds and metal salts were also found to regulate the differential expression of laccase isozymes from both the Ganoderma sp. Laccase isozymes induced in the presence of copper from G. lucidum MDU-7 were purified by gel-based (native-PAGE) purification method. The purity of laccase isozymes was checked by zymogram and SDS-PAGE. The SDS-PAGE of purified proteins confirmed the multimeric nature of laccase isozymes...
2017: Frontiers in Microbiology
K M Polyakov, S Gavryushov, S Ivanova, T V Fedorova, O A Glazunova, A N Popov, O V Koroleva
The laccase from Steccherinum murashkinskyi is a member of the large family of multicopper oxidases that catalyze the oxidation of a wide range of organic and inorganic substrates, accompanied by the reduction of dioxygen to water. The reducing properties of X-ray radiation and the high quality of the laccase crystals allow the study of the catalytic reduction of dioxygen to water directly in a crystal. A series of diffraction data sets with increasing absorbed radiation dose were collected from a single crystal of Steccherinum murashkinskyi laccase at 1...
May 1, 2017: Acta Crystallographica. Section D, Structural Biology
Seung-Ok Yang, Hong Sodaneath, Jung-In Lee, Hyekyeng Jung, Jin-Hee Choi, Hee Wook Ryu, Kyung-Suk Cho
The mycoremediation has been considered as a promising method for decolorizing dye wastewater. To explore new bioresource for mycoremediation, a new white-rot fungus that could decolorize various dyes commonly used in textile industries was isolated, and its ligninolytic enzyme activity and decolorization capacity were characterized. The isolated CBR43 was identified as Trametes versicolor based on the morphological properties of its fruit body and spores, as well as through partial 18S rDNA gene sequences...
May 2, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
Andrea Luna-Acosta, Paco Bustamante, Hélène Thomas-Guyon, Beñat Zaldibar, Urtzi Izagirre, Ionan Marigómez
The impact of dispersed crude oil and dispersant on adult Pacific oysters, Crassostrea gigas, was evaluated through an integrative biomarker approach including (1) biochemical (plasma catecholase- and laccase-type phenoloxidase and superoxide dismutase), (2) histological (digestive cell lysosomal responses, digestive gland histopathology) and (3) physiological (flesh condition index) endpoints in the haemolymph and digestive gland. Adult oysters were exposed to non-contaminated water (control), chemically-dispersed oil (Brut Arabian Light), mechanically-dispersed oil and dispersant (FINASOL®) alone for 2days, and further depurated in non-contaminated water for 4weeks...
April 26, 2017: Science of the Total Environment
Fei Zheng, Qi An, Ge Meng, Xue-Jun Wu, Yu-Cheng Dai, Jing Si, Bao-Kai Cui
A novel laccase (Tolacc-T) from white rot fungus Trametes orientalis was enriched to apparent homogeneity with a specific activity of 20.667U/mg protein and recovery yield of 47.33%. The SDS-PAGE gave a single band indicating that Tolacc-T appears as a monomeric protein with a molecular mass of 44.0kDa. Domain structure analysis revealed that Tolacc-T contained a typical copper II binding domain and shared three potential N-glycosylation sites, but had no copper I binding domain, demonstrating that the enzyme is really a laccase, but a novel laccase...
April 26, 2017: International Journal of Biological Macromolecules
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