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https://www.readbyqxmd.com/read/28820431/high-throughput-screening-assay-for-laccase-engineering-toward-lignosulfonate-valorization
#1
David Rodríguez-Escribano, Felipe de Salas, Isabel Pardo, Susana Camarero
Lignin valorization is a pending issue for the integrated conversion of lignocellulose in consumer goods. Lignosulfonates (LS) are the main technical lignins commercialized today. However, their molecular weight should be enlarged to meet application requirements as additives or dispersing agents. Oxidation of lignosulfonates with fungal oxidoreductases, such as laccases, can increase the molecular weight of lignosulfonates by the cross-linking of lignin phenols. To advance in this direction, we describe here the development of a high-throughput screening (HTS) assay for the directed evolution of laccases, with lignosulfonate as substrate and the Folin-Ciocalteau reagent (FCR), to detect the decrease in phenolic content produced upon polymerization of lignosulfonate by the enzyme...
August 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28820042/biodegradation-of-remazol-brilliant-blue-r-using-isolated-bacterial-culture-staphylococcus-sp-k2204
#2
Velayutham Karthikeyan, Madhava Anil Kumar, Pushparaj Mohanapriya, Thanarasu Amudha, Devaraj Thiruselvi, Periyasamy Karthik, Subramanian Sivanesan
Staphylococcus sp. K2204, a bacterial isolate, was employed in this work to decolorize Remazol Brilliant Blue R (RBBR), which belongs to the anthraquinone class of textile dye. Staphylococcus sp. K2204 biodegraded 100 mg/L RBBR at 37°C, under static condition with the help of extracellular laccase and peroxidases. The products of RBBR degradation were characterized using analytical tools including mass spectral technique. The phytotoxicity tests evaluated the toxicity of RBBR and the products of biodegradation...
August 18, 2017: Environmental Technology
https://www.readbyqxmd.com/read/28818759/increased-cell-wall-thickness-of-endodermis-and-protoxylem-in-aeluropus-littoralis-roots-under-salinity-the-role-of-lac4-and-per64-genes
#3
Behrouz Barzegargolchini, Ali Movafeghi, Ali Dehestani, Pooyan Mehrabanjoubani
Enhanced cell wall lignification is one of the major salinity tolerance strategies in the roots of halophytes. A deep insight into the exact root developmental system in halophytes may be of great importance for understanding plant salt tolerance mechanisms. In this work the developmental and anatomical changes in the roots of halophyte Aeluropus littoralis along with expression patterns of two genes encoding for cell wall laccase (LAC4) and peroxidase (PER64) were investigated. The plants were treated with 0, 300 and 600mM NaCl and root samples were collected 3, 6 and 9days after treatment (DAT)...
August 4, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28816607/investigation-of-a-green-process-for-the-polymerization-of-catechin
#4
Ayşe Ezgi Ünlü, Brinda Prasad, Kishan Anavekar, Paul Bubenheim, Andreas Liese
Flavonoids are polyphenolic secondary plant metabolites which possess antioxidant and anti-inflammatory properties. Besides, they have been shown to exhibit increased antioxidant properties in their polymerized form. Catechins are one of the attractive class of flavonoids which belong to the group of flavan-3-ols. Polymerization of catechins have been investigated in numerous studies indicating the requirement of certain amount of organic solvent to provide the solubility of the monomer. However, many research projects have been conducted recently in order to replace toxic organic contaminants of the processes with environmentally friendly solvents...
August 17, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28810222/bio-electro-oxidation-of-indigo-carmine-by-using-microporous-activated-carbon-fiber-felt-as-anode-and-bioreactor-support
#5
Luane Ferreira Garcia, Ana Claudia Rodrigues Siqueira, Germán Sanz Lobón, Jossano Saldanha Marcuzzo, Benevides Costa Pessela, Eduardo Mendez, Telma Alves Garcia, Eric de Souza Gil
The bioremediation and electro-oxidation (EO) processes are included among the most promising cleaning and decontamination mechanisms of water. The efficiency of bioremediation is dictated by the biological actuator for a specific substrate, its suitable immobilization and all involved biochemical concepts. The EO performance is defined by the anode efficiency to perform the complete mineralization of target compounds and is highlighted by the low or null use of reagent. Recently, the combination of both technologies has been proposed...
August 10, 2017: Chemosphere
https://www.readbyqxmd.com/read/28809813/molecular-mechanisms-underlying-inhibitory-binding-of-alkylimidazolium-ionic-liquids-to-laccase
#6
Jianliang Sun, Hao Liu, Wenping Yang, Shicheng Chen, Shiyu Fu
Water-miscible alkylimidazolium ionic liquids (ILs) are "green" co-solvents for laccase catalysis, but generally inhibit enzyme activity. Here, we present novel insights into inhibition mechanisms by a combination of enzyme kinetics analysis and molecular simulation. Alkylimidazolium cations competitively bound to the TI Cu active pocket in the laccase through hydrophobic interactions. Cations with shorter alkyl chains (C₂~C₆) entered the channel inside the pocket, exhibiting a high compatibility with laccase (competitive inhibition constant Kic = 3...
August 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28806550/discrepant-responses-of-the-electron-transfer-capacity-of-soil-humic-substances-to-irrigations-with-wastewaters-from-different-sources
#7
Wenbing Tan, Yuan Zhang, Beidou Xi, Xiaosong He, Rutai Gao, Caihong Huang, Hui Zhang, Dan Li, Xinyu Zhao, Meng Li, Li Li, Jie Jiang, Guoan Wang
An increasing area of agricultural land is irrigated with wastewater worldwide due to the scarcity of fresh water resources. Whether wastewater irrigations can affect the electron transfer capacity (ETC) of natural organic matter in soils is unclear. In this study, we assess the responses of the electron-accepting capacity (EAC) and electron-donating capacity (EDC) of soil humic substances (HS) to irrigations with wastewaters from different sources. We show that the EAC of soil HS increases and the EDC of soil HS decreases after irrigation with domestic wastewater...
August 11, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28805314/evolved-%C3%AE-factor-prepro-leaders-for-directed-laccase-evolution-in-saccharomyces-cerevisiae
#8
Ivan Mateljak, Thierry Tron, Miguel Alcalde
Although the functional expression of fungal laccases in Saccharomyces cerevisiae has proven to be complicated, the replacement of signal peptides appears to be a suitable approach to enhance secretion in directed evolution experiments. In this study, twelve constructs were prepared by fusing native and evolved α-factor prepro-leaders from S. cerevisiae to four different laccases with low-, medium- and high-redox potential (PM1L from basidiomycete PM1; PcL from Pycnoporus cinnabarinus; TspC30L from Trametes sp...
August 14, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28803111/laccase-enzyme-detoxifies-hydrolysates-and-improves-biogas-production-from-hemp-straw-and-miscanthus
#9
Michel Schroyen, Stijn W H Van Hulle, Sander Holemans, Han Vervaeren, Katleen Raes
The impact of various phenolic compounds, vanillic acid, ferulic acid, p-coumaric acid and 4-hydroxybenzoic acid on anaerobic digestion of lignocellulosic biomass (hemp straw and miscanthus) was studied. Such phenolic compounds have been known to inhibit biogas production during anaerobic digestion. The different phenolic compounds were added in various concentrations: 0, 100, 500, 1000 and 2000mg/L. A difference in inhibition of biomethane production between the phenolic compounds was noted. Hydrolysis rate, during anaerobic digestion of miscanthus was inhibited up to 50% by vanillic acid, while vanillic acid had no influence on the initial rate of biogas production during the anaerobic digestion of hemp straw...
July 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28798768/laccase-13-regulates-seed-setting-rate-by-affecting-hydrogen-peroxide-dynamics-and-mitochondrial-integrity-in-rice
#10
Yang Yu, Quan-Feng Li, Jin-Ping Zhang, Fan Zhang, Yan-Fei Zhou, Yan-Zhao Feng, Yue-Qin Chen, Yu-Chan Zhang
Seed setting rate is one of the most important components of rice grain yield. To date, only several genes regulating setting rate have been identified in plant. In this study, we showed that laccase-13 (OsLAC13), a member of laccase family genes which are known for their roles in modulating phenylpropanoid pathway and secondary lignification in cell wall, exerts a regulatory function in rice seed setting rate. OsLAC13 expressed in anthers and promotes hydrogen peroxide production both in vitro and in the filaments and anther connectives...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28798337/the-laccase-like-reactivity-of-manganese-oxide-nanomaterials-for-pollutant-conversion-rate-analysis-and-cyclic-voltammetry
#11
Xinghao Wang, Jiaoqin Liu, Ruijuan Qu, Zunyao Wang, Qingguo Huang
Nanostructured manganese oxides, e.g. MnO2, have shown laccase-like catalytic activities, and are thus promising for pollutant oxidation in wastewater treatment. We have systematically compared the laccase-like reactivity of manganese oxide nanomaterials of different crystallinity, including α-, β-, γ-, δ-, and ɛ-MnO2, and Mn3O4, with 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) and 17β-estradiol (E2) as the probing substrates. The reaction rate behaviors were examined with regard to substrate oxidation and oxygen reduction to evaluate the laccase-like catalysis of the materials, among which γ-MnO2 exhibits the best performance...
August 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28794933/biosurfactant-and-enzyme-mediated-crude-oil-degradation-by-pseudomonas-stutzeri-na3-and-acinetobacter-baumannii-mn3
#12
Punniyakotti Parthipan, Punniyakotti Elumalai, Kuppusamy Sathishkumar, Devaraj Sabarinathan, Kadarkarai Murugan, Giovanni Benelli, Aruliah Rajasekar
The present study focuses on the optimization of biosurfactant (BS) production using two potential biosurfactant producer Pseudomonas stutzeri NA3 and Acinetobacter baumannii MN3 and role of enzymes in the biodegradation of crude oil. The optimal conditions for P. stutzeri NA3 and A. baumannii MN3 for biodegradation were pH of 8 and 7; temperature of 30 and 40 °C, respectively. P. stutzeri NA3 and A. baumannii MN3 produced 3.81 and 4.68 g/L of BS, respectively. Gas chromatography mass spectrometry confirmed that BS was mainly composed of fatty acids...
October 2017: 3 Biotech
https://www.readbyqxmd.com/read/28780270/development-of-natural-cellulase-inhibitor-mediated-intensified-biological-pretreatment-technology-using-pleurotus-florida-for-maximum-recovery-of-cellulose-from-paddy-straw-under-solid-state-condition
#13
Manickam Naresh Kumar, Rajarathinam Ravikumar, Senniyappan Thenmozhi, Muthuvelu Kirupa Sankar
Inhibitor mediated intensified bio-pretreatment (IMBP) technology using natural cellulase inhibitor (NCI) for maximum cellulose recovery from paddy straw was studied. Pretreatment was carried out under solid state condition. Supplementation of 8% NCI in pretreatment medium improves cellulose recovery and delignification by 1.2 and 1.5-fold respectively, compared to conventional bio-pretreatment due to inhibition of 61% of cellulase activity in IMBP. Further increase in NCI concentration showed negative effect on Pleurotus florida growth and suppress the laccase productivity by 1...
July 22, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28775395/enzymatic-oligomerization-and-polymerization-of-arylamines-state-of-the-art-and-perspectives
#14
REVIEW
Gordana Ćirić-Marjanović, Maja Milojević-Rakić, Aleksandra Janošević-Ležaić, Sandra Luginbühl, Peter Walde
The literature concerning the oxidative oligomerization and polymerization of various arylamines, e.g., aniline, substituted anilines, aminonaphthalene and its derivatives, catalyzed by oxidoreductases, such as laccases and peroxidases, in aqueous, organic, and mixed aqueous organic monophasic or biphasic media, is reviewed. An overview of template-free as well as template-assisted enzymatic syntheses of oligomers and polymers of arylamines is given. Special attention is paid to mechanistic aspects of these biocatalytic processes...
2017: Chemické Zvesti
https://www.readbyqxmd.com/read/28763958/electroanalysis-and-laccase-based-biosensor-on-the-determination-of-phenolic-content-and-antioxidant-power-of-honey-samples
#15
Jerônimo Raimundo de Oliveira Neto, Stefani Garcia Rezende, Gérman Sanz Lobón, Telma Alves Garcia, Isaac Yves Lopes Macedo, Luane Ferreira Garcia, Virgínia Farias Alves, Ieda Maria Sapateiro Torres, Mariângela Fontes Santiago, Fernando Schmidt, Eric de Souza Gil
Honey is a functional food widely consumed. Thus, the evaluation of honey samples to determine its phenolic content and antioxidant capacity (AOC) is relevant to determine its quality. Usually AOC is performed by spectrophotometric methods, which lacks reproducibility and practicality. In this context, the electroanalytical methods offer higher simplicity and accuracy. Hence, the aim of this work was to use of electroanalytical tools and laccase based biosensor on the evaluation of AOC and total phenol content (TPC) of honey samples from different countries...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28763254/decolorization-of-textile-dyes-in-an-air-lift-bioreactor-inoculated-with-bjerkandera-adusta-obr105
#16
Hong Sodaneath, Jung-In Lee, Seung-Ok Yang, Hyekyeng Jung, Hee Wook Ryu, Kyung-Suk Cho
A new decolorizing white-rot fungus, OBR105, was isolated from Mount Odae in South Korea and identified by the morphological characterization of its fruit body and spores and partial 18s rDNA sequences. The ligninolytic enzyme activity of OBR105 was studied to characterize their decolorizing mechanism using a spectrophotometric enzyme assay. For the evaluation of the decolorization capacity of OBR105, the isolate was incubated in an erlenmeyer flask and in an airlifte bioreator with potato dextrose broth (PDB) medium supplemented with each dye...
August 1, 2017: Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering
https://www.readbyqxmd.com/read/28751382/draft-genome-sequence-of-anoxybacillus-sp-strain-uark-01-a-new-thermophilic-lignin-utilizing-bacterium-isolated-from-soil-in-arkansas-usa
#17
Thamir H Alkahem Albalawi, Douglas D Rhoads, Ravi D Barabote
The draft genome of Anoxybacillus sp. strain UARK-01, a novel lignin-utilizing thermophilic soil bacterium, represents the first sequence of an Anoxybacillus isolate from the United States. The genome was sequenced using the Illumina MiSeq platform, de novo assembled using SeqMan NGen, and annotated at NCBI. The genome sequence revealed genes for laccase and lignocellulose degradation enzymes.
July 27, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28751274/a-step-forward-in-laccase-exploitation-recombinant-production-and-evaluation-of-techno-economic-feasibility-of-the-process
#18
Cinzia Pezzella, Valerio Guido Giacobelli, Vincenzo Lettera, Giuseppe Olivieri, Paola Cicatiello, Giovanni Sannia, Alessandra Piscitelli
Protein heterologous production offers viable opportunities to tailor laccase properties to specific industrial needs. The high redox potential laccase POXA1b from Pleurotus ostreatus was chosen as case study of marketable enzyme, due to its desirable properties in terms of activity/stability profile, and already assessed applicability. POXA1b was heterologously produced in Pichia pastoris by investigating the effect of inducible and constitutive expression systems on both the yield and the cost of its production...
July 24, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28745876/ionic-liquids-as-tool-to-improve-enzymatic-organic-synthesis
#19
REVIEW
Toshiyuki Itoh
Ionic liquids (ILs) have now been acknowledged as reaction media for biotransformations. The first three examples were reported in this field in 2000, and since then, numerous applications have been reported for biocatalytic reactions using ILs. Two topics using ILs for enzymatic reactions have been reviewed from the standpoint of biocatalyst mediating organic synthesis; the first is "Biocatalysis in Ionic Liquids" which includes various types of biocatalytic reactions in ILs (section 2): (1) recent examples of lipase-mediated reactions using ILs as reaction media for biodiesel oil production and for sugar ester production, (2) oxidase-catalyzed reactions in ILs, (3) laccase-catalyzed reactions, (4) peroxidase-catalyzed reactions, (4) cytochrome-mediated reactions, (5) microbe-mediated hydrations, (6) protease-catalyzed reactions, (8) whole cell mediated asymmetric reduction of ketones, (10) acylase-catalyzed reactions, (11) glycosylation or cellulase-mediated hydrolysis of polysaccharides, (12) hydroxynitrile lyase-catalyzed reaction, (13) fluorinase or haloalkane dehydrogenase-catalyzed reaction, (14) luciferase-catalyzed reactions, and (15) biocatalytic promiscuity of enzymatic reactions for organic synthesis using ILs...
August 9, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28738694/laccase-a-green-catalyst-for-the-biosynthesis-of-poly-phenols
#20
Jing Su, Jiajia Fu, Qiang Wang, Carla Silva, Artur Cavaco-Paulo
Laccases (benzene diol: oxidoreductases, EC 1.10.3.2) are able to catalyze the oxidation of various compounds containing phenolic and aniline structures using dissolved oxygen in water. Laccase structural features and catalytic mechanisms focused on the polymerization of aromatic compounds are reported. A description about the most recent research on the biosynthesis of chemicals and polymers is made. Selected applications of this technology are considered as well as the advantages, shortcomings and future needs related with the use of laccases...
July 24, 2017: Critical Reviews in Biotechnology
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