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Kim Min, Seung Cheol Jee, Jung-Suk Sung, Avinash A Kadam
Owing to the ubiquitous availability and simple biocatalysis, the anti-proliferative laccase holds enormous opportunities for anti-cancer applications. However, accessing efficient and specific (super-magnetically targetable) new delivery system for anti-proliferative laccase is vital step towards laccase based anti-cancer approach. Therefore, in this investigation, super-magnetized (Fe3 O4 ) and chitosan (CS) functionalized halloysite nanotubes (HNTs) (termed as Fe3 O4 -HNTs-CS) was facile synthesized. Further, laccase from Trametes versicolor was immobilized on Fe3 O4 -HNTs-CS (termed as Fe3 O4 -HNTs-CS-Lac)...
June 15, 2018: International Journal of Biological Macromolecules
Wei Li, Jingwen Yu, Zixin Li, Wen-Bing Yin
Laccases, multicopper oxidoreductases, are mainly produced in white-rot fungi and are considered as ideal green catalysts in industrial and biotechnological applications. However, the development of laccases is limited due to the slow growth of natural laccase producing strains and the low expression levels of laccases. In this study, we designed three regulation strategies for laccase gene expression in the model fungus Aspergillus nidulans. By introducing various promoters in front of the laccase gene pslcc from the white-rot fungus Pycnoporus sanguineus, we found that the laccase gene with the original promoter had effective expression in A...
June 12, 2018: Science China. Life Sciences
Dani Permana, Kosuke Minamihata, Masahiro Goto, Noriho Kamiya
The site-specific cross-linking of functional proteins creates macromolecular assemblies that exhibit unique biochemical and/or physicochemical properties. Herein, we explored the potential of laccase as a biocatalyst for the site-specific cross-linking of tyrosine-tagged proteins. Trametes sp. laccase (TL) was selected as the cross-linking catalyst, and Escherichia coli alkaline phosphatase (BAP) and antibody-binding proteins (pG2 pAs) were employed as model proteins. The protein models were genetically fused to a peptide tag containing a tyrosine residue (Y-tag) at the N- and/or C-termini...
June 12, 2018: Journal of Bioscience and Bioengineering
Fardin Mehrabian, Hossein Kamani, Gholam Hossein Safari, Ghorban Asgari, Seyed Davoud Ashrafi
The removal of Direct Blue 71 (DB71), by laccase (EC, p-bezenediol:dioxygen oxidoreductases) enzyme in presence of 2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), in aqueous solution was investigated. Data on this article focused on the optimizing and modeling of DB71 removal by Response surface method (RSM) based on Box-Behnken design (BBD), through studying the effective variables as follows: ABTS dose (0.05-0.2 mM), Laccase dose (0.05-0.2 U mL-1 ), and pH (3-7). The results of experimental showed that laccase was able to removal DB71 with removal percentage of 83% at concentration of 50 mg L-1 after 30 min incubation in presence of ABTS 0...
August 2018: Data in Brief
Caterina Riccardi, Challa Kumar, Rajeswari Kasi, Shelby McCormick
We report a potentially universal approach for enzyme attachment to cellulose that significantly enhances enzyme stability while retaining high activity, and involves no chemical functionalization of cellulose. In our design, bovine serum albumin (BSA) was interlocked in cellulose to form a protein-friendly surface (named BSA-Paper), while also providing COOH and NH2 groups for subsequent attachment of enzymes. The desired enzyme is then mixed with additional BSA and interlocked on BSA-Paper. The 2nd layer dilutes and crosslinks the enzyme for improved stability...
June 13, 2018: Angewandte Chemie
Daniel Bello-Gil, Emma Roig-Molina, Jennifer Fonseca, María Dolores Sarmiento-Ferrández, Marcela Ferrándiz, Esther Franco, Elena Mira, Beatriz Maestro, Jesús M Sanz
The presence of synthetic dyes in wastewaters generated by the textile industry constitutes a serious environmental and health problem that urges the scientific community on an appropriate action. As a proof-of-concept, we have developed a novel approach to design enzymatic bioreactors with the ability to decolorize dye solutions through the immobilization of the bacterial CueO laccase-like multicopper oxidase from Escherichia coli on polyhydroxybutyrate (PHB) beads by making use of the BioF affinity tag. The decolorization efficiency of the system was characterized by a series of parameters, namely maximum enzyme adsorption capacity, pH profile, kinetic constants, substrate range, temperature and bioreactor recycling...
June 12, 2018: Microbial Biotechnology
Hossein Kamani, Gholam Hossein Safari, Ghorban Asgari, Seyed Davoud Ashrafi
In this article, three variables including laccase dose, 2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) dose and pH were used to modeling of Direct Red 81 (DR81) elimination from aqueous solutions by laccase-mediated system. Obtained data indicated that the predicted and experimental values were close for DR81 elimination, and the regression was also able to give a good prediction of response for DR81 elimination ( R -Squared = 0.9983). From the experimental, the highest elimination of the DR81 was 95...
June 2018: Data in Brief
Rong Zhou, Zhaokun Liu, Yeni N Zhang, Tzi Bun Ng, Fang Liu
For centuries, mushrooms have been widely used as traditional Chinese medicine in Asia. Apart from polysaccharides and some small-molecule components, such as flavones, ployphenols and terpenes, mushrooms produce a large number of pharmaceutically active proteins, which have become popular sources of natural antitumor, antimicrobial, immunoenhancing agents. These bioactive proteins include lectins, laccases, ribosome inactivating proteins (RIPs), nucleases, and fungal immunomodulatory proteins (FIPs). The review is to summarize the characters of structure and bioactivities involved in antitumor, antiviral, antifungal, antibacterial and immunoenhancing activities of proteins from edible mushrooms, to better understand their mechanisms, and to direct research...
June 12, 2018: Current Protein & Peptide Science
Haibin Yuan, Lin Chen, Feng F Hong, Meifang Zhu
Properties of bacterial nanocellulose (BNC) produced by four different strains were studied and compared for laccase immobilization. Scanning electron microscope inspection indicated the four types of BNC had obvious differences in fiber density, diameter and distribution. BNC hydrogel demonstrated the highest fracture stress of 2.44 Mpa and the highest Young's modulus of 12.76 Mpa. Brunauer-Emmett-Teller analysis suggested the four BNC had significant difference in specific surface area, total pore volume and average pore size...
September 15, 2018: Carbohydrate Polymers
Olga A Glazunova, Konstantin M Polyakov, Konstantin V Moiseenko, Sergei A Kurzeev, Tatyana V Fedorova
Laccases are multicopper oxidases that catalyze oxidation of a wide range of organic and inorganic substrates accompanied by the reduction of dioxygen to water. The physicochemical and catalytic properties of two new fungal laccases from basidiomycetes Antrodiella faginea (AfL) and Steccherinum murashkinskyi (SmL) with middle redox potential of the T1 copper site were studied. The X-ray structures of AfL and SmL were solved at 1.75 Å and 0.95 Å, respectively. The oxidized state of copper ions in the active site was observed in AfL structure, while the mixture of oxidized and reduced states was observed in SmL structure...
June 8, 2018: International Journal of Biological Macromolecules
Line Munk, Mogens Larsen Andersen, Anne S Meyer
Laccases (EC catalyze oxidation of phenolic groups in lignin to phenoxyl radicals during reduction of O2 to H2 O. Here, we examine the influence on this radical formation of mediators which are presumed to act by shuttling electrons between the laccase and the subunits in lignin that the enzyme cannot approach directly. Treatments of three different lignins with laccase-mediator-systems (LMS) including laccases derived from Trametes versicolor and Myceliophthora thermophila, respectively, and four individual mediators, 1-hydroxybenzotriazole (HBT), N-hydroxyphthalimide (HPI), 2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPO), and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) were assessed by real time electron paramagnetic resonance measurements...
September 2018: Enzyme and Microbial Technology
Sonica Sondhi, Randhir Kaur, Supreet Kaur, Palki Sahib Kaur
Decolourization of textile effluent is always being a problem of major concern for the safe disposal of effluent water in river stream. Due to intense colour of the effluent water, even after the treatment, disposal of the water in river stream is under strict environmental regulation. Laccase, a natural oxidase, have the miraculous power to decolourize different dyes with or without mediators. However, stability and cost of implementation of the enzyme at industrial conditions make the entire process unfeasible at industrial scale...
June 6, 2018: International Journal of Biological Macromolecules
Gerald N Presley, Ellen Panisko, Samuel O Purvine, Jonathan S Schilling
Wood-degrading fungi use a sequence of oxidative and hydrolytic mechanisms to loosen lignocellulose and then release and metabolize embedded sugars. These temporal sequences have recently been mapped at high resolution using directional growth on wood wafers, revealing previously obscured dynamics as fungi progressively colonize wood. Here, we applied secretomics in the same wafer design to track temporal trends on aspen decayed by fungi with distinct nutritional modes: two brown rot-type (BR) fungi ( Postia placenta , Gloeophyllum trabeum ) and two white rot (WR) ( Stereum hirsutum , Trametes versicolor )...
June 8, 2018: Applied and Environmental Microbiology
Baojun Wu, Jill Gaskell, Benjamin W Held, Cristina Toapanta, Thu Vuong, Steven Ahrendt, Anna Lipzen, Jiwei Zhang, Jonathan S Schilling, Emma Master, Igor V Grigoriev, Robert A Blanchette, Dan Cullen, David S Hibbett
Wood-decaying fungi tend to have characteristic substrate ranges that partly define their ecological niche. Fomitopsis pinicola is a brown rot species of Polyporales that is reported on 82 species of softwoods and 42 species of hardwoods. We analyzed gene expression levels and RNA editing profiles of F. pinicola from submerged cultures with ground wood powder (sampled at five days) or solid wood wafers (sampled at ten and thirty days), using aspen, pine, and spruce substrates (aspen was used only in submerged cultures)...
June 8, 2018: Applied and Environmental Microbiology
Simona Giacobbe, Cinzia Pezzella, Vincenzo Lettera, Giovanni Sannia, Alessandra Piscitelli
Apple pomace, potato peels, and coffee silverskin are attractive agrofood wastes for the production of biofuels and chemicals, due to their abundance and carbohydrate content. As lignocellulosic biomasses, their conversion is challenged by the presence of lignin that prevents hydrolysis of polysaccharides, hence demanding a pretreatment step. In this work, the effectiveness of Pleurotus ostreatus laccases (with and without mediator) to remove lignin, improving the subsequent saccharification, was assessed. Optimized conditions for sequential protocol were set up for all agrofood wastes reaching delignification and detoxification yields correlated with high saccharification...
June 1, 2018: Bioresource Technology
Miguel Peixoto de Almeida, Pedro Quaresma, Susana Sousa, Cláudia Couto, Inês Gomes, Ludwig Krippahl, Ricardo Franco, Eulália Pereira
Adsorption of enzymes to nanoparticles and the mechanisms responsible for enzyme activity modulation of adsorbed enzymes are not well understood. In this work, gold nanoparticles were used for electrostatic adsorption of a plant-derived laccase. Adsorption constants were determined by four independent techniques: dynamic light scattering, electrophoretic light scattering, agarose gel electrophoresis and fluorescence quenching. Stable bionanoconjugates were formed with log K in the range 6.8-8.9. An increase in enzyme activity was detected, in particular at acidic and close to neutral pH values, a feature that expands the useful pH range of the enzyme...
June 8, 2018: Physical Chemistry Chemical Physics: PCCP
Haitao Nie, Ziyu Wang, Jihao You, Gang Zhu, Hengchang Wang, Feng Wang
Objective: The effects of Pleurotus ostreatus on the feed utilization improvement of broad bean stalks (BBS), rape straw (RS), paddy straw (PS) and corn stalk (CS) was examined. Methods: The four roughages were co-cultured with Pleurotus ostreatus. The chemical composition; enzyme activities of laccase, carboxymethylcellulase (CMCase) and xylanase; carbohydrate and protein fractions (based on CNCPS) were assessed at different days after inoculation (7, 14, 21, 28 d) and un-inoculated roughages (control, 0 d)...
May 31, 2018: Asian-Australasian Journal of Animal Sciences
Ranjit Das, Guiying Li, Bixian Mai, Taicheng An
Laccase has been applied extensively as a biocatalyst to remove different organic pollutants. This study characterized a spore-laccase from the bisphenol A (BPA)-degrading strain Bacillus sp. GZB. The spore-laccase was encoded with 513 amino acids, containing spore coat protein A (CotA). It showed optimal activity at 70 °C and pH = 7.2 in presence of 2, 6-dimethoxyphenol. At 60 °C, optimal activity was also seen at pH = 3.0 and pH = 6.8 with 2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate) and syringaldazine, respectively...
June 4, 2018: Science of the Total Environment
Marine Breitwieser, Evelyne Vigneau, Amélia Viricel, Vanessa Becquet, Camille Lacroix, Marina Erb, Valérie Huet, Carine Churlaud, Stéphane Le Floch, Benoit Guillot, Marianne Graber, Hélène Thomas
Increasing activity along the French Atlantic coast has led to chronic pollution with, in particular, mixtures of contaminants such as hydrocarbons, phytosanitary products, PCBs and heavy metals. Based on previous research, pollution biomarkers were used in this study as they can indicate health status when monitoring the impact of pollutants on coastal species such as the marine bivalve Mimachlamys varia. Mollusc bivalves were sampled in March 2016, in open and semi-open areas (a harbour zone), from thirteen sites which differed in terms of their level of pollution, and were located along the Atlantic coast from Brittany down to the Nouvelle-Aquitaine region...
June 2, 2018: Science of the Total Environment
Yujie Liu, Zhifeng Liu, Guangming Zeng, Ming Chen, Yilin Jiang, Binbin Shao, Zhigang Li, Yang Liu
Some surfactants can enhance the removal of phenol by laccase (Lac) in various industrial effluents. Their behavior and function in the biodegradation of phenolic wastewater have been experimentally reported by many researchers, but the underlying molecular mechanism is still unclear. Therefore, the interaction mechanisms of phenol with Lac from Trametes versicolor were investigated in the presence or absence of Triton X-100 (TX100) or rhamnolipid (RL) by molecular docking and molecular dynamics (MD) simulations...
May 22, 2018: Journal of Hazardous Materials
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