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https://www.readbyqxmd.com/read/28643461/single-enzyme-biofuel-cells
#1
Chao Ji, Jingwei Hou, Kai Wang, Yun Hau Ng, Vicki Chen
Here, we firstly demonstrate that the intramolecular electron transfer within signle enzyme molecule is an important alternative pathway which can be harnessed to generate electricity. By decoupling the redox reactions within a single type of enzyme (e.g., Trametes versicolor laccase), we harvested electricity efficiently from unconventional fuels including recalcitrant pollutants (e.g. bisphenol-A and hydroquinon) in a single-laccase biofuel cell. The intramolecular electron-harnessing concept was further demonstrated with other enzymes, including power generation during CO2 bioconversion to formate catalyzed by formate dehydrogenase from Candida boidinii...
June 23, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28634851/multi-step-biocatalytic-depolymerization-of-lignin
#2
Pere Picart, Haifeng Liu, Philipp M Grande, Nico Anders, Leilei Zhu, Jürgen Klankermayer, Walter Leitner, Pablo Domínguez de María, Ulrich Schwaneberg, Anett Schallmey
Lignin is a biomass-derived aromatic polymer that has been identified as a potential renewable source of aromatic chemicals and other valuable compounds. The valorization of lignin, however, represents a great challenge due to its high inherent functionalization, what compromises the identification of chemical routes for its selective depolymerization. In this work, an in vitro biocatalytic depolymerization process is presented, that was applied to lignin samples obtained from beech wood through OrganoCat pretreatment, resulting in a mixture of lignin-derived aromatic monomers...
June 21, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28624938/synergetic-integration-of-laccase-and-versatile-peroxidase-with-magnetic-silica-microspheres-towards-remediation-of-biorefinery-wastewater
#3
Dhanya Vishnu, Gerard Neeraj, Ramachandran Swaroopini, Ravi Shobana, Vaidyanathan Vinoth Kumar, Hubert Cabana
In this study, a tailor-made biocatalyst consisting of a co-immobilized lignolytic enzyme cascade on multi-functionalized magnetic silica microspheres (MSMS) was developed. Physical adsorption was the most promising strategy for the synthesis of individual immobilized laccase (IL), immobilized versatile peroxidase (IP), as well as co-immobilized laccase (Lac) and versatile peroxidase (VP) with an enzyme activity recovery of about 79, 93, 27, and 27.5%, respectively. Similarly, the biocatalytic load of 116, 183, 23...
June 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28624475/oxidoreductases-on-their-way-to-industrial-biotransformations
#4
REVIEW
Angel T Martínez, Francisco J Ruiz-Dueñas, Susana Camarero, Ana Serrano, Dolores Linde, Henrik Lund, Jesper Vind, Morten Tovborg, Owik M Herold-Majumdar, Martin Hofrichter, Christiane Liers, René Ullrich, Katrin Scheibner, Giovanni Sannia, Alessandra Piscitelli, Cinzia Pezzella, Mehmet E Sener, Sibel Kılıç, Willem J H van Berkel, Victor Guallar, Maria Fátima Lucas, Ralf Zuhse, Roland Ludwig, Frank Hollmann, Elena Fernández-Fueyo, Eric Record, Craig B Faulds, Marta Tortajada, Ib Winckelmann, Jo-Anne Rasmussen, Mirjana Gelo-Pujic, Ana Gutiérrez, José C Del Río, Jorge Rencoret, Miguel Alcalde
Fungi produce heme-containing peroxidases and peroxygenases, flavin-containing oxidases and dehydrogenases, and different copper-containing oxidoreductases involved in the biodegradation of lignin and other recalcitrant compounds. Heme peroxidases comprise the classical ligninolytic peroxidases and the new dye-decolorizing peroxidases, while heme peroxygenases belong to a still largely unexplored superfamily of heme-thiolate proteins. Nevertheless, basidiomycete unspecific peroxygenases have the highest biotechnological interest due to their ability to catalyze a variety of regio- and stereo-selective monooxygenation reactions with H2O2 as the source of oxygen and final electron acceptor...
June 14, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28621383/peach-and-cherry-agroindustrial-wastes-new-and-economic-sources-for-the-production-of-lignocellulolytic-enzymes
#5
Merve Akpinar, Raziye Urek
Pleurotus eryngii was incubated on both peach and cherry agroindustrial wastes by solid state fermentation for 30 days without/with pretreatment conditions. The lignocellulosic substrates were pretreated with dilute acid and alkaline solutions, hot water before incubation. The maximum carboxymethyl cellulase and xylanase activities peaked on 3rd and 5th days under control conditions of both wastes, respectively. The highest laccase and manganese peroxidase activities reached to their maximum on 17th day as 2193...
June 2017: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/28620814/oil-palm-fiber-biodegradation-physico-chemical-and-structural-relationships
#6
Farah Nadia Omar, Halimatun Saadiah Hafid, Azhari Samsu Baharuddin, Mohd Afandi P Mohammed, Jaafar Abdullah
X-ray microtomography results revealed that delignification process damaged the oil palm fibers, which correlated well with reduction of lignin components and increase of the phenolic content. Biodegradation investigation of natural fibers normally focuses on physico-chemical analysis, with less emphasis on physical aspect like fiber structures affect from microbial activity. In this work, the performance of Pycnoporus sanguineus to delignify oil palm empty fruit bunch fibers through solid-state fermentation utilizing various ratio of POME sludge was reported...
June 15, 2017: Planta
https://www.readbyqxmd.com/read/28620217/secretory-laccase-1-in-bemisia-tabaci-med-is-involved-in-whitefly-plant-interaction
#7
Chun-Hong Yang, Jian-Yang Guo, Dong Chu, Tian-Bo Ding, Ke-Ke Wei, Deng-Fa Cheng, Fang-Hao Wan
The whitefly Bemisia tabaci is a phloem-feeding pest that lives predominantly on herbaceous species and causes serious damage to hosts. Whitefly saliva is thought to contain proteins that modulate plant defences and facilitate feeding. A predicted secreted protein, laccase 1 (LAC1), was found in the salivary gland transcriptome of B. tabaci and might be existed in the watery saliva of B. tabaci. As LAC1 has a potential role in detoxification of secondary plant compounds in insects, we speculated that it may participate in the insect's response to plant defences...
June 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28606354/the-stlac2-gene-is-required-for-cell-wall-integrity-dhn-melanin-synthesis-and-the-pathogenicity-of-setosphaeria-turcica
#8
Shuangxin Ma, Keke Cao, Ning Liu, Chuan Meng, Zhiyan Cao, Dongqing Dai, Hui Jia, Jinpin Zang, Zhiyong Li, Zhimin Hao, Shouqin Gu, Jingao Dong
Laccases are blue multicopper oxidases, play important roles in various biological processes. These processes include fungal dihydroxynaphthalene (DHN)-melanin biosynthesis and pathogenicity, cellular growth, morphogenesis, and differentiation. This study investigated functions of the laccase gene StLAC2 in Setosphaeria turcica. The Δlac2 mutant colony color was distinct from that of the S. turcica wild-type (WT) isolate, and the mutants exhibited defective conidial formation. In contrast to the WT, the mutants exhibited a lighter color on the 2, 2-azino-di-[3-ethylbenzo-thia-zolin-sulphonate] (ABTS) plates, and the intracellular laccase activity was lower...
June 2017: Fungal Biology
https://www.readbyqxmd.com/read/28606353/extracellular-redox-cycling-and-hydroxyl-radical-production-occurs-widely-in-lichenized-ascomycetes
#9
Calvin E Moyo, Richard P Beckett, Tatyana V Trifonova, Farida V Minibayeva
Some free-living Ascomycetes and white and brown rot Basidiomycetes can generate hydroxyl radicals using extracellular redox cycling. However, the mechanisms of hydroxyl radical production differ between white and brown rot Basidiomycetes, and are unknown for Ascomycetes. Here, we present a survey of extracellular hydroxyl radical production by a range of lichenized Ascomycetes. Results show that given a quinone and chelated ferric ions, many lichens can readily produce hydroxyl radicals, and this is accompanied by the reduction of Fe(3)(+) to Fe(2+)...
June 2017: Fungal Biology
https://www.readbyqxmd.com/read/28602730/recombinant-expression-of-a-laccase-from-coriolopsis-gallica-in-pichia-pastoris-using-a-modified-%C3%AE-factor-preproleader
#10
Mayra Avelar, Clarita Olvera, Denise Aceves-Zamudio, Jorge Luis Folch, Marcela Ayala
In this work we communicate the heterologous expression of a laccase from Coriolopsis gallica in Pichia pastoris. This enzyme has been reported to efficiently degrade a variety of pollutants such as industrial dyes. The expression strategy included using a previously reported modified α-factor preproleader for enhanced secretion and pAOX1, a methanol-responsive promoter. Methanol concentration, copper salts concentration and temperature were varied in order to enhance laccase expression in this heterologous system...
June 5, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28594931/de-novo-transcriptome-assembly-and-quantification-reveal-differentially-expressed-genes-between-soft-seed-and-hard-seed-pomegranate-punica-granatum-l
#11
Hui Xue, Shangyin Cao, Haoxian Li, Jie Zhang, Juan Niu, Lina Chen, Fuhong Zhang, Diguang Zhao
Pomegranate (Punica granatum L.) belongs to Punicaceae, and is valued for its social, ecological, economic, and aesthetic values, as well as more recently for its health benefits. The 'Tunisia' variety has softer seeds and big arils that are easily swallowed. It is a widely popular fruit; however, the molecular mechanisms of the formation of hard and soft seeds is not yet clear. We conducted a de novo assembly of the seed transcriptome in P. granatum L. and revealed differential gene expression between the soft-seed and hard-seed pomegranate varieties...
2017: PloS One
https://www.readbyqxmd.com/read/28593945/human-lacc1-increases-innate-receptor-induced-responses-and-a-lacc1-disease-risk-variant-modulates-these-outcomes
#12
Amit Lahiri, Matija Hedl, Jie Yan, Clara Abraham
Functional consequences for most inflammatory disease-associated loci are incompletely defined, including in the LACC1 (C13orf31) region. Here we show that human peripheral and intestinal myeloid-derived cells express laccase domain-containing 1 (LACC1); LACC1 is expressed in both the cytoplasm and mitochondria. Upon NOD2 stimulation of human macrophages, LACC1 associates with the NOD2-signalling complex, and is critical for optimal NOD2-induced signalling, mitochondrial ROS (mtROS) production, cytokine secretion and bacterial clearance...
June 8, 2017: Nature Communications
https://www.readbyqxmd.com/read/28589227/laccase-mediated-synthesis-of-lignin-core-hyperbranched-copolymers
#13
Mark D Cannatelli, Arthur J Ragauskas
Lignin, one of the major chemical constituents of woody biomass, is the second most abundant biopolymer found in nature. The pulp and paper industry has long produced lignin on the scale of millions of tons annually as a by-product of the pulping process, and the dawn of cellulosic ethanol production has further contributed to this amount. Historically, lignin has been perceived as a waste material and burned as a fuel for the pulping process. However, recent research has been geared toward developing cost-effective technologies to convert lignin into valuable commodities...
June 6, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28589226/the-first-acidobacterial-laccase-like-multicopper-oxidase-revealed-by-metagenomics-shows-high-salt-and-thermo-tolerance
#14
Luka Ausec, Francesca Berini, Carmine Casciello, Mariana Silvia Cretoiu, Jan Dirk van Elsas, Flavia Marinelli, Ines Mandic-Mulec
Metagenomics is a powerful tool that allows identifying enzymes with novel properties from the unculturable component of microbiomes. However, thus far only a limited number of laccase or laccase -like enzymes identified through metagenomics has been subsequently biochemically characterized. This work describes the successful bio-mining of bacterial laccase-like enzymes in an acidic bog soil metagenome and the characterization of the first acidobacterial laccase-like multicopper oxidase (LMCO). LMCOs have hitherto been mostly studied in fungi and some have already found applications in diverse industries...
June 6, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28588605/mir397-laccase-gene-mediated-network-improves-tolerance-to-fenoxaprop-p-ethyl-in-beckmannia-syzigachne-and-oryza-sativa
#15
Lang Pan, Hongwei Zhao, Qin Yu, Lianyang Bai, Liyao Dong
Herbicide resistance can be either target-site or non-target-site based. The molecular mechanisms underlying non-target-site resistance (NTSR) are poorly understood, especially at the level of gene expression regulation. MicroRNAs (miRNAs) represent key post-transcriptional regulators of eukaryotic gene expression and play important roles in stress responses. In this study, the miR397 gene from Beckmannia syzigachne (referred to as bsy-miR397) was functionally characterized to determine its role in regulating fenoxaprop-P-ethyl resistance...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28588565/diversity-of-ligninolytic-enzymes-and-their-genes-in-strains-of-the-genus-ganoderma-applicable-for-biodegradation-of-xenobiotic-compounds
#16
Giselle Torres-Farradá, Ana M Manzano León, François Rineau, Lucía L Ledo Alonso, María I Sánchez-López, Sofie Thijs, Jan Colpaert, Miguel Ramos-Leal, Gilda Guerra, Jaco Vangronsveld
White-rot fungi (WRF) and their ligninolytic enzymes (laccases and peroxidases) are considered promising biotechnological tools to remove lignin related Persistent Organic Pollutants from industrial wastewaters and contaminated ecosystems. A high diversity of the genus Ganoderma has been reported in Cuba; in spite of this, the diversity of ligninolytic enzymes and their genes remained unexplored. In this study, 13 native WRF strains were isolated from decayed wood in urban ecosystems in Havana (Cuba). All strains were identified as Ganoderma sp...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28585469/heterologous-expression-of-the-pleurotus-ostreatus-mnp3-gene-by-the-laccase-gene-promoter-in-lentinula-edodes
#17
Toshitsugu Sato, Toshikazu Irie, Fumihiko Yoshino
Lentinula edodes (shiitake), which have a powerful ligninolytic system, is one of the most important edible mushrooms in Asia. In this study, we introduced the manganese peroxidase (MnP, EC 1.11.1.13) gene from Pleurotus ostreatus driven by L. edodes laccase 1 gene promoter into L. edodes for expression. The resulting transformant expressed the recombinant gene and showed a higher level of MnP activity than that of the wild-type strain.
June 6, 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28585171/assessing-the-biodegradation-of-polycyclic-aromatic-hydrocarbons-and-laccase-production-by-new-fungus-trematophoma-sp-utmc-5003
#18
Hamid Moghimi, Rezvan Heidary Tabar, Javad Hamedi
Environmental pollution caused by petroleum compounds has become a global concern. The aim of this study was to evaluate the indigenous fungal isolates in Iran for biodegradation of crude oil pollutants. In order to isolate fungal strains, the soil samples were enriched in minimal salts medium (MSM) with 1% crude oil and then the crude oil degradation was measured by total petroleum hydrocarbon (TPH) assay. The degradation of hydrocarbons compounds was also analysed by FT-IR and HPLC, and the activity of peroxidase enzyme and biosurfactant production were also measured...
July 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28580232/mineralization-of-a-sulfonated-textile-dye-reactive-red-31-from-simulated-wastewater-using-pellets-of-aspergillus-bombycis
#19
Razia Khan, M H Fulekar
BACKGROUND: Reactive Red 31, applied extensively in the commercial textile industry, is a hazardous and persistent azo dye compound often present in dye manufacturing and textile industrial effluents. Aspergillus bombycis strain was isolated from dye contaminated zones of Gujarat Industrial Development Corporation, Vatva, Ahmedabad, India. The decolorization potential was monitored by the decrease in maximum absorption of the dye using UV-visible spectroscopy. Optimization of physicochemical conditions was carried out to achieve maximum decolorization of Reactive Red 31 by fungal pellets...
2017: Bioresources and Bioprocessing
https://www.readbyqxmd.com/read/28578045/impact-of-laccase-on-the-colour-stability-of-structured-oil-in-water-emulsions
#20
Catherine K Y Chan, Benjamin Zeeb, David Julian McClements, Jochen Weiss
The optical properties of food emulsions play a key role in determining their perceived quality because they are the first sensory cue that many consumers receive. The purpose of the current study was to investigate the impact of a cross-linking enzyme (laccase) on the appearance of structured oil-in-water emulsions containing a lipophilic model colorant (Nile red). A layer-by-layer electrostatic deposition approach was used to prepare oil-in-water emulsions stabilized by interfacial protein-pectin complexes under acidic conditions (pH3...
July 2017: Food Research International
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