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https://www.readbyqxmd.com/read/28441744/production-of-laccase-by-a-new-myrothecium-verrucaria-md-r-16-isolated-from-pigeon-pea-cajanus-cajan-l-millsp-and-its-application-on-dye-decolorization
#1
Jiao Sun, Na Guo, Li-Li Niu, Qing-Fang Wang, Yu-Ping Zang, Yuan-Gang Zu, Yu-Jie Fu
The present study was conducted to screen a laccase-producing fungal endophyte, optimize fermentation conditions, and evaluate the decolorization ability of the laccase. A new fungal endophyte capable of laccase-producing was firstly isolated from pigeon pea and identified as Myrothecium verrucaria based on a ITS-rRNA sequences analysis. Meanwhile, various fermentation parameters on the laccase production were optimized via response surface methodology (RSM). The optimal fermentation conditions were a fermentation time of five days, temperature 30 °C and pH 6...
April 23, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28439850/heterologous-expression-of-a-streptomyces-cyaneus-laccase-for-biomass-modification-applications
#2
Selin Ece, Camilla Lambertz, Rainer Fischer, Ulrich Commandeur
Laccases are used for the conversion of biomass into fermentable sugars but it is difficult to produce high yields of active laccases in heterologous expression systems. We overcame this challenge by expressing Streptomyces cyaneus CECT 3335 laccase in Escherichia coli (ScLac) and we achieved a yield of up to 104 mg L(-1) following purification by one-step affinity chromatography. Stability and activity assays using simple lignin model substrates showed that the purified enzyme preparation was active over a broad pH range and at high temperatures, suggesting it would be suitable for biomass degradation...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28438269/stone-eating-fungi-mechanisms-in-bioweathering-and-the-potential-role-of-laccases-in-black-slate-degradation-with-the-basidiomycete-schizophyllum-commune
#3
Julia Kirtzel, Daniela Siegel, Katrin Krause, Erika Kothe
Many enzymes, such as laccases, are involved in the saprotrophic lifestyle of fungi and the effects of those may be linked to enhanced bioweathering on stone surfaces. To test this hypothesis, we studied the decomposition of kerogen-enriched lithologies, especially with black slate containing up to 20% of Corg. Indeed, a formation of ditches with attached hyphal material could be observed. To address enzymes involved, proteomics was performed and one group of enzymes, the multicopper oxidase family members of laccases, was specifically investigated...
2017: Advances in Applied Microbiology
https://www.readbyqxmd.com/read/28437742/biological-treatment-of-model-dyes-and-textile-wastewaters
#4
Alicia Paz, Julia Carballo, María José Pérez, José Manuel Domínguez
Previous works conducted in our laboratory, reveled that Bacillus aryabhattai DC100 produce ligninolytic enzymes such as laccases and/or peroxidases, opening new applications in different bioprocesses, including the treatment of disposal residues such as dyestuffs from textile processing industries. This work described the degradation of three commercial model dyes Coomassie Brilliant Blue G-250 (CBB), Indigo Carmine (IC) and Remazol Brilliant Blue R (RBBR) under different culture media and operational conditions...
April 18, 2017: Chemosphere
https://www.readbyqxmd.com/read/28433724/whole-genome-de-novo-sequencing-of-wood-rot-fungus-fomitopsis-palustris-atcc62978-with-both-a-cellulolytic-and-ligninolytic-enzyme-system
#5
Chang-Young Hong, Su-Yeon Lee, Sun-Hwa Ryu, Myungkil Kim
Fomitopsis palustris is a model brown rot fungus causing destructive wood decay based on the cellulase system. Endoglucanase secreted by F. palustris hydrolyzes cellulose in both the crystalline and amorphous form. In this study, whole-genome sequencing was conducted to identify genes related to F. palustris cellulose degradation and their functions. We determined the 43-Mb complete draft genome of F. palustris (ATCC 62978), comprising 14,592 predicted gene models. Gene annotation provided crucial information about the location and function of protein-encoding genes...
April 19, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28431786/-ligninolytic-enzyme-production-by-white-rot-fungi-during-paraquat-herbicide-degradation
#6
Reyna L Camacho-Morales, José Luis Gerardo-Gerardo, Karina Guillén Navarro, José E Sánchez
Paraquat is a widely used herbicide in agriculture. Its inappropriate use and wide distribution represents a serious pollution problem for soil and water. White rot fungi are capable of degrading pollutants having a similar structure to that of lignin, such as paraquat. This study evaluated the degradation effect of paraquat on the production of ligninolytic enzymes by white rot fungi isolated from the South of Mexico. Six fungal strains showed tolerance to the herbicide in solid culture. Three of the six evaluated strains showed levels of degradation of 32, 26 and 47% (Polyporus tricholoma, Cilindrobasidium laeve and Deconica citrispora, respectively) after twelve days of cultivation in the presence of the xenobiotic...
April 18, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/28422049/regulation-of-anthocyanin-biosynthesis-in-purple-leaves-of-zijuan-tea-camellia-sinensis-var-kitamura
#7
Lingxia Wang, Dezhuo Pan, Meng Liang, Yakubu Saddeeq Abubakar, Jian Li, Jinke Lin, Shipin Chen, Wei Chen
Plant anthocyanin biosynthesis is well understood, but the regulatory mechanism in purple foliage tea remains unclear. Using isobaric tag for relative and absolute quantification (iTRAQ), 815 differential proteins were identified in the leaves of Zijuan tea, among which 20 were associated with the regulation of anthocyanin metabolism. We found that the abundances of anthocyanin synthesis-related enzymes such as chalcone synthase, chalcone isomerase, dihydroflavonol 4-reductase and anthocyanin synthetase, as well as anthocyanin accumulation-related UDP-glucosyl transferase and ATP-binding cassette (ABC) transporters in the purple leaves were all significantly higher than those in the green leaves...
April 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28421142/plackett-burman-design-for-rgilcc1-laccase-activity-enhancement-in-pichia-pastoris-concentrated-enzyme-kinetic-characterization
#8
Edwin D Morales-Álvarez, Claudia M Rivera-Hoyos, Ángela M Cardozo-Bernal, Raúl A Poutou-Piñales, Aura M Pedroza-Rodríguez, Dennis J Díaz-Rincón, Alexander Rodríguez-López, Carlos J Alméciga-Díaz, Claudia L Cuervo-Patiño
Laccases are multicopper oxidases that catalyze aromatic and nonaromatic compounds with concomitant reduction of molecular oxygen to water. They are of great interest due to their potential biotechnological applications. In this work we statistically improved culture media for recombinant GILCC1 (rGILCC1) laccase production at low scale from Ganoderma lucidum containing the construct pGAPZαA-GlucPost-Stop in Pichia pastoris. Temperature, pH stability, and kinetic parameter characterizations were determined by monitoring concentrate enzyme oxidation at different ABTS substrate concentrations...
2017: Enzyme Research
https://www.readbyqxmd.com/read/28419836/enzymatic-behavior-of-laccase-following-interaction-with-%C3%AE-cd-and-immobilization-into-pcl-nanofibers
#9
M Fatih Canbolat, Hasan Basri Savas, Fatih Gultekin
This study examines the effects of CD use on enzymatic activity, following enzyme immobilization into nanofibers. There is almost no research available on the change in enzyme activity following interaction with cyclodextrin and electrospun nanofiber mats together. Laccase enzyme was immobilized into nanofibrous structures by various techniques, with and without γ-CD addition, and the enzymatic activity of the laccase was analyzed. SEM, XRD, and FTIR analyses were used for the characterization of the resulting structures...
April 15, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28418639/supermagnetically-tuned-halloysite-nanotubes-functionalized-with-aminosilane-for-covalent-laccase-immobilization
#10
Avinash A Kadam, Jiseon Jang, Dae Sung Lee
Halloysite nanotubes (HNTs) were tuned with supermagnetic Fe3O4 (M-HNTs) and functionalized with γ-aminopropyltriethoxysilane (APTES) (A-M-HNTs). Gluteraldehyde (GTA) was linked to A-M-HNTs (A-M-HNTs-GTA) and explored for covalent laccase immobilization. The structural characterization of M-HNTs, A-M-HNTs, and A-M-HNTs-GTA-immobilized laccase (A-M-HNTs-GTA-Lac) was determined by X-ray photoelectron spectroscopy, field-emission high-resolution transmission electron microscopy, a magnetic property measurement system, and thermogavimetric analyses...
April 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28415001/degradation-of-synthetic-fragrances-by-laccase-mediated-system
#11
Laura Vallecillos, Yumna Sadef, Francesc Borrull, Eva Pocurull, Kai Bester
Laccase mediator systems are important biodegradation agents as the rate of reaction could be enhanced in the presence of redox mediators. In the present study the commercial enzyme laccase from Trametes versicolor and the redox mediator 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were used for the biotransformation of the synthetic fragrances 1-(1,2,3,4,5,6,7,8-octahydro-2,3,8,8,-tetramethyl-2-naphthyl)ethan-1-one (Iso-E-Super, OTNE), 1,3,4,6,7,8,-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-[g]-2-benzopyran (Galaxolide, HHCB), 7-acetyl-1,1,3,4,4,6-hexamethyl-1,2,3,4-tetrahydronaphtalene (Tonalide, AHTN) and the transformation product of HHCB, 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylcyclopenta-[g]-2-benzopyran-1-one (Galaxolidone, HHCB-lactone) in water...
April 4, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28411810/multivariate-curve-resolution-applied-to-kinetic-spectroscopic-data-matrices-dye-determination-in-foods-by-means-of-enzymatic-oxidation
#12
Valeria Boeris, Juan A Arancibia, Alejandro C Olivieri
In this work, the combination of chemometric techniques with kinetic-spectroscopic data allowed quantifying two dyes (tartrazine and carminic acid) in complex matrices as mustard, ketchup, asparagus soup powder, pumpkin soup powder, plum jam and orange-strawberry juice. Quantitative analysis was performed without the use of tedious sample pretreatment, due to the achievement of the second-order advantage. The results obtained showed an improvement in simplicity, speed and cost with respect to usual separation techniques, allowing to properly quantifying these dyes obtaining limits of detection below 0...
July 1, 2017: Talanta
https://www.readbyqxmd.com/read/28411649/nanoarmoring-of-enzymes-by-interlocking-in-cellulose-fibers-with-poly-acrylic-acid
#13
Caterina M Riccardi, Rajeswari M Kasi, Challa V Kumar
A simple method for interlocking glucose oxidase (GOx) and horseradish peroxidase (HRP) in cellulose fibers using poly(acrylic acid) (PAA) as an armor around the enzyme, without any need for activation of the cellulose support, is reported here. The resulting enzyme paper is an inexpensive, stable, simple, wearable, and washable biosensor. PAA functions as a multifunctional tether to interlock the enzyme molecules around the paper fibers so that the enzymes are protected against thermal/chemical denaturation and not released from the paper when washed with a detergent...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28408867/visualization-of-snare-mediated-hemifusion-between-giant-unilamellar-vesicles-arrested-by-myricetin
#14
Paul Heo, Joon-Bum Park, Yeon-Kyun Shin, Dae-Hyuk Kweon
Neurotransmitters are released within a millisecond after Ca(2+) arrives at an active zone. However, the vesicle fusion pathway underlying this synchronous release is yet to be understood. At the center of controversy is whether hemifusion, in which outer leaflets are merged while inner leaflets are still separated, is an on-pathway or off-pathway product of Ca(2+)-triggered exocytosis. Using the single vesicle fusion assay, we recently demonstrated that hemifusion is an on-pathway intermediate that immediately proceeds to full fusion upon Ca(2+) triggering...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28397501/layer-by-layer-assembled-laccase-enzyme-on-stimuli-responsive-membranes-for-chloro-organics-degradation
#15
Rupam Sarma, Md Saiful Islam, Anne-Frances Miller, Dibakar Bhattacharyya
Functionalized membranes provide versatile platforms for the incorporation of biocatalysts and nanostructured materials for efficient and benign environmental remediation. The existing techniques for remediating chloro-organics in water consist of both physical and chemical means mostly using metal oxide-based catalysts, despite associated environmental concerns. To offer bioinspired remediation as an alternative, we herein demonstrate a layer-by-layer approach to immobilize laccase enzyme onto pH-responsive functionalized membranes for the degradation of chloro-organics in water...
April 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28393483/upgrading-laccase-production-and-biochemical-properties-strategies-and-challenges
#16
REVIEW
Brandt Bertrand, Fernando Martínez Morales, María R Trejo-Hernández
Improving laccases continues to be crucial in novel biotechnological developments and industrial applications, where they are concerned. This review breaks down and explores the potential of the strategies (conventional and modern) that can be used for laccase enhancement (increased production and upgraded biochemical properties such as stability and catalytic efficiency). The challenges faced with these approaches are briefly discussed. We also shed light on how these strategies merge and give rise to new options and advances in this field of work...
April 10, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28390277/enhancement-in-multiple-lignolytic-enzymes-production-for-optimized-lignin-degradation-and-selectivity-in-fungal-pretreatment-of-sweet-sorghum-bagasse
#17
Vartika Mishra, Asim K Jana, Mithu Maiti Jana, Antriksh Gupta
The objective of this work was to study the increase in multiple lignolytic enzyme productions through the use of supplements in combination in pretreatment of sweet sorghum bagasse (SSB) by Coriolus versicolor such that enzymes act synergistically to maximize the lignin degradation and selectivity. Enzyme activities were enhanced by metallic salts and phenolic compound supplements in SSF. Supplement of syringic acid increased the activities of LiP, AAO and laccase; gallic acid increased MnP; CuSO4 increased laccase and PPO to improve the lignin degradations and selectivity individually, higher than control...
March 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28389772/anoxybacillus-sp-strain-uark-01-a-new-thermophilic-soil-bacterium-with-hyperthermostable-alkaline-laccase-activity
#18
Thamir H Al-Kahem Al-Balawi, Adam L Wood, Alexis Solis, Ted Cooper, Ravi D Barabote
We describe the isolation and characteristics of a novel thermophilic bacterium from soil. The organism is a member of the Anoxybacillus genus based on phylogenetic analysis of the 16S rRNA gene. The 16S rRNA of the organism shares >99% sequence identity with those of two species, Anoxybacillus rupiensis and A. geothermalis. We named this isolate as Anoxybacillus sp. strain UARK-01. UARK-01 grows optimally in the presence of oxygen at 55 °C and pH 8. It grew excellently in the presence of lignin as the sole carbon source...
April 8, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28384218/a-novel-multicopper-oxidase-laccase-from-cyanobacteria-purification-characterization-with-potential-in-the-decolorization-of-anthraquinonic-dye
#19
Sumbul Afreen, Tooba Naz Shamsi, Mohd Affan Baig, Nadeem Ahmad, Sadaf Fatima, M Irfan Qureshi, Md Imtaiyaz Hassan, Tasneem Fatma
A novel extracellular laccase enzyme produced from Spirulina platensis CFTRI was purified by ultrafiltration, cold acetone precipitation, anion exchange and size exclusion chromatography with 51.5% recovery and 5.8 purification fold. The purified laccase was a monomeric protein with molecular mass of ~66 kDa that was confirmed by zymogram analysis and peptide mass fingerprinting. The optimum pH and temperature of the enzyme activity was found at 3.0 and 30°C using ABTS as substrate but the enzyme was quite stable at high temperature and alkaline pH...
2017: PloS One
https://www.readbyqxmd.com/read/28382652/a-new-promising-phylogenetic-marker-to-study-the-diversity-of-fungal-communities-the-glycoside-hydrolase-63-gene
#20
L Pérez-Izquierdo, E Morin, J P Maurice, F Martin, A Rincón, M Buée
In molecular ecology, the development of efficient molecular markers for fungi remains an important research domain. Nuclear ribosomal internal transcribed spacer (ITS) region was proposed as universal DNA barcode marker for Fungi, but this marker was criticized for idel-induced alignment problems and its potential lack of phylogenetic resolution. Our main aim was to develop a new phylogenetic gene and a putative functional marker, from single-copy gene, to describe fungal diversity. Thus, we developed a series of primers to amplify a polymorphic region of the Glycoside Hydrolase GH63 gene, encoding exo-acting α-glucosidases, in Basidiomycetes...
April 6, 2017: Molecular Ecology Resources
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