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Embryo morphokinetics

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https://www.readbyqxmd.com/read/30539737/do-immature-and-mature-sibling-oocytes-recovered-from-stimulated-cycles-have-the-same-reproductive-potential
#1
L Escrich, Y Galiana, N Grau, F Insua, N Soler, A Pellicer, M J Escribá
RESEARCH QUESTION: How can laboratory and clinical outcomes of spontaneously, early maturing germinal-vesicle oocytes and sibling in-vivo-matured (metaphase II [MII]) oocytes be quantified and compared? DESIGN: A prospective, non-randomized intra-cohort study of oocytes from women aged 38 years or younger, with six or fewer MII oocytes and four or more germinal vesicles retrieved. No indication was identified for genetic tests or oocyte or embryo cryopreservation...
December 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/30477915/autologous-mitochondrial-transfer-as-a-complementary-technique-to-intracytoplasmic-sperm-injection-to-improve-embryo-quality-in-patients-undergoing-in-vitro-fertilization-a-randomized-pilot-study
#2
Elena Labarta, Maria José de Los Santos, Sonia Herraiz, Maria José Escribá, Alicia Marzal, Anna Buigues, Antonio Pellicer
OBJECTIVE: To study if autologous mitochondrial transfer (AUGMENT) improves outcome in patients with previously failed in vitro fertilization (IVF). DESIGN: Randomized, controlled, triple-blind, experimental study. SETTING: Private infertility center, Valencian Institute of Infertility (IVI-RMA), Valencia, Spain. PATIENT(S): Infertile women ≤42 years of age, body mass index <30 kg/m2 , antimüllerian hormone ≥4 pmol/L, >5 million/mL motile sperm, at least one previous IVF with at least five metaphase oocytes (MIIs) collected, and low embryo quality...
November 24, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/30448216/morphokinetic-characteristics-of-embryos-derived-from-in-vitro-matured-oocytes-and-their-in-vivo-matured-siblings-after-ovarian-stimulation
#3
Tamar Margalit, Avi Ben-Haroush, Roni Garor, Naomi Kotler, Dania Shefer, Natalia Krasilnikov, Moran Tzabari, Galia Oron, Yoel Shufaro, Onit Sapir
RESEARCH QUESTION: Does delayed maturation of aspirated metaphase I (MI) oocytes, completed in vitro, adversely affect early embryo development? DESIGN: Time-lapse microscopy was used to compare morphokinetic variables between embryos derived from oocytes with delayed maturation after ovarian stimulation and from in-vivo-matured metaphase II (MII) sibling oocytes from the same IVF and intracytoplasmic sperm injection cycle. RESULTS: A total of 1545 injected oocytes in 169 cycles from 149 patients were included...
November 10, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/30371742/is-differences-in-embryo-morphokinetic-development-significantly-associated-with-human-embryo-sex
#4
Bo Huang, Xinling Ren, Lixia Zhu, Li Wu, Huiping Tan, Na Guo, Yulan Wei, Juan Hu, Qun Liu, Wen Chen, Jing Liu, Dan Li, Shujie Liao, Lei Jin
To date, very few studies have reported on the relationship between live birth gender and embryo development kinetics. This study included 1,735 women undergoing IVF or ICSI by using a time-lapse system. Finally, a total of 228 qualified patients with 100% implantation and known live birth information were included in the analysis. There were 174 male live births and 134 female live births. The time to 3 (t3), 4 (t4) and 5 (t5) cell development of male embryos was significantly shorter/earlier than female embryos (P < 0...
October 26, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/30344090/the-relationship-between-preimplantation-morphokinetics-of-human-embryos-and-sex-chromosome-pattern
#5
Ali Zeyad, Bandar Al-Abdulkareem, Mohamad Eid Hammadeh
The aim of this study was to determine the relationship between the gender of human embryos and chronological changes during the two pronuclear (2PN) embryonic stage and blastocyst formation on day five after injection using time-lapse imaging and preimplantation genetic testing. A total of 120 couples who underwent intracytoplasmic sperm injection with preimplantation genetic testing were included in the study. Only normal embryos (n = 416) were enrolled in this study. Time-lapse imaging data of male (n = 227) and female (n = 189) embryo progression was carried out to estimate the times of initial appearance of the 2PN stage, fading times and day five blastulation rates...
October 18, 2018: Reproductive Biology
https://www.readbyqxmd.com/read/30314885/time-lapse-imaging-algorithms-rank-human-preimplantation-embryos-according-to-the-probability-of-live-birth
#6
Simon Fishel, Alison Campbell, Sue Montgomery, Rachel Smith, Lynne Nice, Samantha Duffy, Lucy Jenner, Kathryn Berrisford, Louise Kellam, Rob Smith, Fiona Foad, Ashley Beccles
RESEARCH QUESTION: Can blastocysts leading to live births be ranked according to morphokinetic-based algorithms? DESIGN: Retrospective analysis of 781 single blastocyst embryo transfers, including all patient clinical factors that might be potential confounders for the primary outcome measure of live birth, was weighed using separate multi-variable logistic regression models. RESULTS: There was strong evidence of effect of embryo rank on odds of live birth...
September 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/30301510/follicular-environment-as-a-predictive-tool-for-embryo-development-and-kinetics-in-cattle
#7
Gláucia Pereira Alves, Fernanda Bertuccez Cordeiro, Camila Bruna de Lima, Kelly Annes, Érika Cristina Dos Santos, Jéssica Ispada, Patrícia Kubo Fontes, Marcelo Fabio Gouveia Nogueira, Marcílio Nichi, Marcella Pecora Milazzotto
Follicular fluid composition and the transcription pattern of granulosa cells were analysed to better comprehend associations between embryo development and morphokinetics. Bovine follicles were punctured and their respective follicular fluid and granulosa cells were collected. Cumulus-oocyte complexes derived from these follicles were matured and fertilised in vitro. Embryo morphology and kinetics were evaluated at 40 h after insemination, when embryos were classified as fast (FCL, four or more cells), slow (SCL, 2-3 cells) or non-cleaved (NCL)...
October 10, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/30288481/serum-anti-mullerian-hormone-and-embryo-morphokinetics-detecting-by-time-lapse-imaging-a-comparison-between-the-polycystic-ovarian-syndrome-and-tubal-factor-infertility
#8
Nasim Tabibnejad, Mehrdad Soleimani, Abbas Aflatoonian
Background: Anti-Mullerian hormone (AMH) is considered as a good marker for quantitative evaluation of ovarian response to the stimulation during assisted reproductive technology cycles. Objective: To evaluate the association between serum AMH level and embryo morphokinetics using time-lapse imaging and intracytoplasmic sperm injection (ICSI) outcomes in women with polycystic ovarian syndrome (PCOS). Materials and Methods: We evaluated a total of 547 embryos from 100 women underwent ICSI cycles; 50 women with PCOS and 50 women with tubal factor infertility...
August 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/30259705/high-sperm-dna-fragmentation-delays-human-embryo-kinetics-when-oocytes-from-young-and-healthy-donors-are-microinjected
#9
M Esbert, A Pacheco, S R Soares, D Amorós, M Florensa, A Ballesteros, M Meseguer
BACKGROUND: Time-lapse monitoring (TLM) technology has been implemented in the clinical setting for the culture and selection of human embryos. Many studies have assessed the association between sperm DNA fragmentation (sDNAf) and clinical outcomes after ART, but little is known about the influence of sDNA on embryo morphokinetics. OBJECTIVES: The objective of this retrospective study, which includes 971 embryos from 135 consecutive ICSI cycles (56 cases with own oocytes, 79 with oocytes from young and healthy donors), was to assess if sDNAf has an impact on embryo morphokinetics...
September 26, 2018: Andrology
https://www.readbyqxmd.com/read/30245444/zona-pellucida-birefringence-and-meiotic-spindle-visualization-are-not-related-to-the-time-lapse-detected-embryo-morphokinetics-in-women-with-polycystic-ovarian-syndrome
#10
N Tabibnejad, M Soleimani, A Aflatoonian
OBJECTIVES: Oocyte parameters as well as embryo developmental kinetics can be applied as non-invasive predictors for selection of viable embryos and clinical outcomes. Time-lapse morphokinetic events in conjunction with zona pellucida birefringence (ZPB) and meiotic spindle visualization (MSV) have been evaluated for predicting pregnancy outcome. STUDY DESIGN: We assessed a total of 547 embryos from 100 intra cytoplasmic sperm injection cycles in women with polycystic ovary syndrome (PCOS) and tubal factor infertility...
November 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/30112232/live-birth-from-the-transfer-of-a-severely-fragmented-embryo-observed-by-morphokinetics
#11
S Lahav-Baratz, I Blais, M Koifman, D Ishai, Z Wiener-Megnazi, G Peer, M Dirnfeld
We report a live birth from a heavily fragmented embryo which continued cleavage to a fully expanded blastocyst. A 32-year-old patient underwent 2 IVF cycles without achieving pregnancy. In the first cycle, 2 embryos with fragmentation were transferred; in the second, all embryos were fragmented and no embryo transfer was performed. In a third cycle, 12 oocytes were retrieved and 11 of them were fertilized. On day 2, all 11 embryos started to unwind to fragments. By careful annotation, using the time-lapse EmbryoScope, we observed that one embryo continued division as expected, discarding all fragments aside...
2018: Case Reports in Obstetrics and Gynecology
https://www.readbyqxmd.com/read/30107362/effects-of-opening-the-incubator-on-morphokinetics-in-mouse-embryos
#12
Quyen Nguyen, Sophia Sommer, Brandon Greene, Christine Wrenzycki, Uwe Wagner, Volker Ziller
OBJECTIVE: To evaluate the effects of incubator door openings by the sensitive measurement of morphokinetics using time-lapse imaging. STUDY DESIGN: To mimic lab practice and to evaluate minimal changes in culture conditions a randomized parallel group study with mouse embryos was designed. 249 murine zygotes derived from 31 mice were randomly distributed into two groups. Embryos of the control group were non-invasively monitored with a Primovision time-lapse system (Vitrolife) in the incubator that was completely closed for 5 days, while the conditions for the embryos in the treatment group were interrupted by two door openings per day of 10 s each...
October 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/30031399/is-there-a-correlation-between-follicle-size-and-gene-expression-in-cumulus-cells-and-is-gene-expression-an-indicator-of-embryo-development
#13
Semra Kahraman, Caroline Pirkevi Çetinkaya, Murat Çetinkaya, Mehmet Ali Tüfekçi, Cumhur Gökhan Ekmekçi, Markus Montag
BACKGROUND: In an article published in 2017, we discussed the results of the first part of our study into the morphokinetic development of embryos in relation to follicle diameter and homogeneity of follicular development. Our findings showed that embryos coming from small follicles in heterogeneous cycles had significantly higher rates of arrest or failure to reach blastocyst than embryos coming from large follicles in homogenous cycles. The aim of this further study was to investigate the relationship between follicular size and gene expression of cumulus cells (CCs) and evaluate whether gene expression could be an indicator of embryo development...
July 21, 2018: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29995338/assessing-icsi-outcome-by-combining-non-invasive-indicators-early-time-lapse-morphokinetics-and-apoptosis-in-associated-cumulus-cells-among-women-with-the-polycystic-ovarian-syndrome
#14
Nasim Tabibnejad, Abbas Aflatoonian, Leila Motamedzadeh, Mehrdad Soleimani, Fatemeh Sadeghian-Nodoushan, Ali Reza Talebi
Cumulus cells features and embryo developmental events can be considered as noninvasive indicators for embryo selection and clinical outcomes. A combination of time-lapse morphokinetic parameters and cumulus cell apoptosis in women with polycystic ovarian syndrome (PCOS) was evaluated for predicting pregnancy outcome. We assessed a total of 547 embryos from 100 intracytoplasmic sperm injection (ICSI) cycles. Time-lapse records were interpreted in time to pronuclear fading (tPNf), time to 2 to 8 cells (t2-t8), direct cleavage, reverse cleavage, and also for the presence of multinucleation...
July 11, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29932617/external-validation-of-a-time-lapse-model-a-retrospective-study-comparing-embryo-evaluation-using-a-morphokinetic-model-to-standard-morphology-with-live-birth-as-endpoint
#15
Emma Adolfsson, Sandra Porath, Anna Nowosad Andershed
OBJECTIVE: To validate a morphokinetic implantation model developed for EmbryoScope on embryos with known outcome, compared to standard morphology in a retrospective single center study. METHODS: Morphokinetic annotation of 768 embryos with known outcome between 2013 -2015; corresponding to 116 D3 fresh embryos, 80 D6 frozen blastocysts, and 572 D5 blastocysts, fresh or frozen. The embryos were ranked by the KIDScore into five classes, KID1-5, and grouped into four classes based on standard morphology...
September 1, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29912521/morphology-vs-morphokinetics-a-retrospective-comparison-of-inter-observer-and-intra-observer-agreement-between-embryologists-on-blastocysts-with-known-implantation-outcome
#16
Emma Adolfsson, Anna Nowosad Andershed
OBJECTIVE: Our primary aim was to compare the morphology and morphokinetics on inter- and intra-observer agreement for blastocyst with known implantation outcome. Our secondary aim was to validate the morphokinetic parameters' ability to predict pregnancy using a previous published selection algorithm, and to compare this to standard morphology assessments. METHODS: Two embryologists made independent blinded annotations on two occasions using time-lapse images and morphology evaluations using the Gardner Schoolcraft criteria of 99 blastocysts with known implantation outcome...
September 1, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29882361/influence-of-human-sperm-origin-testicular-or-ejaculated-on-embryo-morphokinetic-development
#17
Filomena Scarselli, Valentina Casciani, Elisabetta Cursio, Saverio Muzzì, Alessandro Colasante, Simona Gatti, Maria Chiara Greco, Pierfrancesco Greco, Maria Giulia Minasi, Ermanno Greco
In this retrospective observational study (October 2014 - July 2016), the impact of sperm origin on embryo morphokinetics and on clinical outcomes after intracytoplasmic sperm injection was evaluated. The developmental kinetics of embryos obtained either with testicular sperm (40 cycles; testicular sperm group) or with thawed donor sperm (26 cycles; donor sperm group) was analysed up to day-3 of culture with a time-lapse incubation system. In the testicular sperm group, all patients were affected by nonobstructive azoospermia...
October 2018: Andrologia
https://www.readbyqxmd.com/read/29790071/impact-of-polar-body-biopsy-on-embryo-morphokinetics-back-to-the-roots-in-preimplantation-genetic-testing
#18
Michael Schenk, Andrea Groselj-Strele, Katharina Eberhard, Elisabeth Feldmeier, Darja Kastelic, Stefanie Cerk, Gregor Weiss
PURPOSE: Polar body biopsy (PBB) is a common technique in preimplantation genetic testing (PGT) to assess the chromosomal status of the oocyte. Numerous studies have been implemented to investigate the impact of biopsies on embryo development; however, information on embryo morphokinetics is still lacking. Hence, we investigated the impact of PBB on morphokinetic parameters in early embryo development. METHODS: Four hundred four embryos (202 PBB, 202 control) were retrospectively analyzed...
August 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29785530/azoospermia-and-embryo-morphokinetics-testicular-sperm-derived-embryos-exhibit-delays-in-early-cell-cycle-events-and-increased-arrest-prior-to-compaction
#19
Nina Desai, Pavinder Gill, Nicholas N Tadros, Jeffrey M Goldberg, Edmund Sabanegh, Tommaso Falcone
PURPOSE: Sperm play an essential role in embryonic genome activation and embryonic progression to blastocyst. In the present work, we focus on development of embryos created as a result of ICSI with testicular or epididymal sperm from azoospermic males and compare this to outcomes from normospermic males. The objective of this study was to determine if sperm origin influences clinical outcomes, the kinetics of embryo development, or the incidence of cleavage anomalies and multinucleation...
July 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29664791/embryo-morphokinetic-characteristics-and-euploidy
#20
Francesca Pennetta, Cristina Lagalla, Andrea Borini
PURPOSE OF REVIEW: The purpose of the current review is to provide an update on time-lapse morphokinetic assessment related to embryo ploidy status. RECENT FINDINGS: The main limitation of the available studies regarding correlation between morphokinetic variables and ploidy is that each embryo is considered as an independent unit whereas recent findings show that embryo kinetics may be affected by patient and ovarian stimulation-related factors, so that clustered data analysis is more appropriate...
June 2018: Current Opinion in Obstetrics & Gynecology
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