Tejas Dharmaraj, Michael J Kratochvil, Julie D Pourtois, Qingquan Chen, Maryam Hajfathalian, Aviv Hargil, Yung-Hao Lin, Zoe Evans, Agnès Oromí-Bosch, Joel D Berry, Robert McBride, Naomi L Haddock, Derek R Holman, Jonas D van Belleghem, Tony H Chang, Jeremy J Barr, Rob Lavigne, Sarah C Heilshorn, Francis G Blankenberg, Paul L Bollyky
UNLABELLED: Extensive efforts are underway to develop bacteriophages as therapies against antibiotic-resistant bacteria. However, these efforts are confounded by the instability of phage preparations and a lack of suitable tools to assess active phage concentrations over time. Here, we use Dynamic Light Scattering (DLS) to measure changes in phage physical state in response to environmental factors and time, finding that phages tend to decay and form aggregates and that the degree of aggregation can be used to predict phage bioactivity...
July 2, 2023: bioRxiv