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Molecular test

Liron Pantanowitz
No abstract text is available yet for this article.
October 14, 2018: Cancer Cytopathology
Sinchita Roy-Chowdhuri
No abstract text is available yet for this article.
October 14, 2018: Cancer Cytopathology
José Villanueva, Yolanda Gimenez-Molina, Luis M Gutiérrez
Cultured bovine chromaffin cells have been tested as a successful neuroendocrine model to study the secretory process. Changes in the dynamics of the secretory vesicles and the exocytotic machinery microdomains could be studied in control and stimulated conditions using appropriate molecular tools such as fluorescent SNARE protein expression or fluorochrome vesicular labeling in these neuroendocrine cells. Since most of these changes occur in or near the plasma membrane, the use of the total internal reflection fluorescent microscopy (TIRFM) and the implement of particle motion analysis could be essential tools to study the structural and dynamic changes of secretory machinery related with its function in this exocytotic cell model...
2019: Methods in Molecular Biology
Xiao Kong, Decai Jin, Xinxin Wang, Fengsong Zhang, Guilan Duan, Huijun Liu, Minghong Jia, Ye Deng
Dibutyl phthalate (DBP) is a typical soil contaminant that is widely used as plasticizer in modern agricultural production. In this study, an experiment was conducted to evaluate fungal community succession in a soil-vegetable ecosystem under different DBP concentrations. By using high-throughput sequencing of the ribosomal internal transcribed spacer (ITS) region, it was shown that DBP contamination caused significant changes to the soil fungal community, in terms of both α and β diversities. The largest changes in fungal α and β diversities were detected under 50 mg/kg DBP concentration at the first day of addition...
October 6, 2018: Chemosphere
Shiyu Song, Haiyan Min, Mengyuan Niu, Lei Wang, Yongzheng Wu, Bin Zhang, Xiufang Chen, Qiao Liang, Yanting Wen, Yong Wang, Long Yi, Hongwei Wang, Qian Gao
BACKGROUND: S1PR1-STAT3 inter-regulatory loop was initially suggested to be oncogenic in several cancer cells. However, the clinical relevance of this mechanism in tumor progression, disease prognosis and drug response was not established. METHODS: The correlations between S1PR1 transcription, overall survival and chemotherapy response of GC patients were tested using a large clinical database. The relevance of S1PR1 expression and STAT3 activation in both tumor tissues and cancer cell lines was also tested...
October 10, 2018: EBioMedicine
Roberto Zenteno-Cuevas, Betzaida Cuevas-Córdoba, Aurora Parissi-Crivelli
INTRODUCTION: Previous knowledge of molecular mechanisms related with multi-drug resistances in tuberculosis is important if molecular diagnostic procedures want to be used in specific geographical regions. For that reason, the aim of this study was to investigate the mutations at rpoB, katG and inhA in multi-drug resistant tuberculosis isolates from Southeast Mexico. METHODS: Isolates of tuberculosis with a confirmed resistance against rifampicin and isoniazid were collected and sequencing analysis was performed of the rpoB rifampicin resistance-determining region, the katG and the encoding region of inhA...
October 10, 2018: Enfermedades Infecciosas y Microbiología Clínica
Fabien Tran, Péter Kovács, Leila Kalantari, Georg K H Madsen, Peter Blaha
A recent study of Mejia-Rodriguez and Trickey [Phys. Rev. A 96 , 052512 (2017)] showed that the deorbitalization procedure (replacing the exact Kohn-Sham kinetic-energy density by an approximate orbital-free expression) applied to exchange-correlation functionals of the meta-generalized gradient approximation (MGGA) can lead to important changes in the results for molecular properties. For the present work, the deorbitalization of MGGA functionals is further investigated by considering various properties of solids...
October 14, 2018: Journal of Chemical Physics
Sven Kähler, Jeppe Olsen
The use of non-orthogonal orbitals allows the construction and use of more compact wave functions than offered by standard methods using orthogonal molecular orbitals; in particular, for molecules containing partly occupied atomic orbitals as present, for example, in transition metal complexes. With the purpose of developing efficient dynamic correlation methods, we discuss several new internal correlation methods employing a reference state containing non-orthogonal active orbitals. The non-orthogonal internally contracted perturbation theory approach is improved in several directions...
October 14, 2018: Journal of Chemical Physics
Giacomo Mulas, Cyril Falvo, Patrick Cassam-Chenaï, Christine Joblin
While powerful techniques exist to accurately account for anharmonicity in vibrational molecular spectroscopy, they are computationally very expensive and cannot be routinely employed for large species and/or at non-zero vibrational temperatures. Motivated by the study of Polycyclic Aromatic Hydrocarbon (PAH) emission in space, we developed a new code, which takes into account all modes and can describe all infrared transitions including bands becoming active due to resonances as well as overtone, combination, and difference bands...
October 14, 2018: Journal of Chemical Physics
Ramon S da Silva, Maikel Y Ballester
Vibrational transitions, induced by collisions between rare-gas atoms and molecules, play a key role in many problems of interest in physics and chemistry. A theoretical investigation of the translation-to-vibration (T-V) energy transfer process in argon atom and sulfur dioxide molecule collisions is presented here. For such a purpose, the framework of the quasi-classical trajectory (QCT) methodology was followed over the range of translational energies 2 ≤ Etr /kcal mol-1 ≤ 100. A new realistic potential energy surface (PES) for the ArSO2 system was developed using pairwise addition for the four-body energy term within the double many-body expansion...
October 14, 2018: Journal of Chemical Physics
Kotar Tadeja, Pirš Mateja, Steyer Andrej, Cerar Tjaša, Šoba Barbara, Skvarc Miha, Poljšak Prijatelj Mateja, Lejko Zupanc Tatjana
OBJECTIVES: A stool sample is a sample of choice for microbiological testing of enteric pathogens causing diarrhoea, but a rectal swab can be a more practical alternative. A prospective observational study was performed to evaluate the diagnostic performance of flocked rectal swab specimens using the syndromic molecular approach to determine the aetiology of diarrhoea in adults. METHODS: We compared the performance of rectal swabs to stool samples as the gold standard in determining viral, bacterial and protozoal pathogens using real-time multiplex PCR as well as standard stool culture...
October 10, 2018: Clinical Microbiology and Infection
Zeynali M Shima, Mohammadian Mahshid, Kheradmand Fatemeh, Fathi-Azarbayjani Anahita, Rasmi Yousef, Esna-Ashari Omid, Malekinejad Hassan
Anti-proliferative, anti-metastatic and anti-angiogenic effects of 17‑allylamino‑17‑demethoxy geldanamycin (17-AAG) were studied alone and in combination with Capecitabine (Cap) and/or Irinotecan (IR) on HT-29 human colorectal carcinoma cells. Expression of MMP-9 (matrix metalloproteinase‑9) and VEGF (vascular endothelial growth factor) mRNA was analyzed by real-time PCR method. The study was further followed by wound scratch assay for migration assessment. Nitric oxide content, MDA generation and total anti-oxidant capacity were also assessed...
October 10, 2018: Gene
Irina A Tolmacheva, Alexey V Nazarov, Daria V Eroshenko, Victoria V Grishko
Synthetic transformations of steroids for drug discovery and improvement of drug effectiveness have been an important part of modern medicinal chemistry and pharmaceutical sciences. Pentacyclic triterpenoids, being represented in the nature by various structures and biogenetically related to steroids, can largely expand the spectrum of biologically active steroidal agents via synthesis of the so-called "triterpenoid-steroid" hybrids. In the presented work, the nitrile anion cyclizations of 3,4-secolupane and 3,4-seco-oleanane nitriles and follow-up synthetic transformations of the cyclized products with formation of the gemm-dimethyl-free A ring "triterpenoid-steroid" hybrids were studied...
October 10, 2018: Steroids
A Jafarizadeh, S A Pourbakhsh, K Tadayon, M Jamshidian
Mycoplasma capricolum subspecies capricolum (Mcc) is one of the causative agents of contagious agalactia (CA), which is an important disease in sheep and goats in the Mediterranean and Middle East countries. Mycoplasma agalactiae is the classic agent of CA in sheep and goats. Mycoplasma mycoides subspecies Capri (Mmc), Mycoplasma capricolum subspecies capricolum (Mcc), and Mycoplasma putrefaciens (Mp) produce a clinically similar disease, more often in goats. The aim of the present study was to detect Mcc in sheep flocks in East Azerbaijan Province of Iran...
December 2017: Archives of Razi Institute
S Sabzevari, G Razmi, A Naghibi, J Khoshnegah
Zoonotic visceral leishmaniasis is caused by Leishmania infantum (L. infantum),and its major reservoir hosts are domestic dogs, most of which are asymptomatic. This study aimed to detect L. infantum spp. in asymptomatic stray dogs by molecular and serological methods in Mashhad, Iran, during 2011-12. In this study, 94 asymptomatic stray dogs were randomly selected and their blood samples were collected for indirect fluorescent antibody testing. Furthermore, tissue samples from all the L. infantum seropositive stray dogs were examined using semi-nested polymerase chain reaction (PCR)...
December 2017: Archives of Razi Institute
E Rahimabadi, Y Asadpour, S A Pourbakhsh, P Sayehban
Contagious agalactia is an infectious syndrome of sheep that is characterized by mastitis with reduction of milk production, arthritis, abortion, and keratoconjunctivitis. The disease is rapidly spread by the contact of the infected animals with the healthy ones. Domestic sheep and goats of both sexes can be infected at an equivalent frequency. Most of the researchers use culture and molecular methods for the isolation and identification of Mycoplasma. Mycoplasma agalactiae is the main cause of the disease in sheep...
December 2017: Archives of Razi Institute
P Mancini, G Bonanno Ferraro, M Iaconelli, E Suffredini, B Valdazo-González, S Della Libera, M Divizia, G La Rosa
AIMS: Human Sapoviruses (HSaVs) are etiological agents of sporadic cases and outbreaks of acute gastroenteritis in humans of all ages. Evidence of worldwide distribution of HSaV has been documented, however little is known about HSaV circulation in Italy. To study their occurrence and genetic diversity a nation-wide environmental surveillance was undertaken. METHODS AND RESULTS: One hundred sixty-six raw sewage samples, collected from 16 wastewater treatment plants throughout Italy, were processed and analysed by a RT-nested-PCR targeting the capsid region, followed by amplicon sequencing...
October 13, 2018: Journal of Applied Microbiology
Traimat Boonthai, Thomas P Loch, Qingli Zhang, Michelle Gunn Van Deuren, Mohamed Faisal, Gary E Whelan, Seth J Herbst
Indigenous small cyprinid fish species play an important role in Great Lakes ecosystems and also comprise the backbone of a multimillion-dollar baitfish industry. Due to their widespread use in sport fisheries of the Laurentian Great Lakes, there are increasing concerns that baitfish may introduce or disseminate fish pathogens. In this study, we evaluated whether baitfish purchased from 78 randomly selected retail bait dealers in Michigan harbored fish viruses. Between September 2015 and June 2016, 5,400 baitfish divided into 90 lots of 60 fish were purchased...
October 12, 2018: Journal of Aquatic Animal Health
Byungkwan Jeong, Jaemin Kim, Taegu Lee, Seong-Woong Kim, Seunghwa Ryu
We propose a theoretical framework to predict the deformation mechanism of the γ-TiAl single crystal without lattice defects by combining the generalized stacking fault energy and the Schmid factor. Our theory is validated against an excellent testbed, the single crystal nanowire, by correctly predicting four major deformation mechanisms, namely, ordinary slip, super slip, twinning, and mixed slip/fracture observed during the tensile and compressive tests along 10 different orientations using molecular dynamics simulations...
October 12, 2018: Scientific Reports
David M Ross, Ilaria S Pagani, Naranie Shanmuganathan, Chung H Kok, John F Seymour, Anthony K Mills, Robin J Filshie, Christopher K Arthur, Phuong Dang, Verity A Saunders, Jodi Braley, Agnes S Yong, David T Yeung, Deborah L White, Andrew P Grigg, Anthony P Schwarer, Susan Branford, Timothy P Hughes
Following the achievement of deep molecular response on tyrosine kinase inhibitors (TKIs), approximately half of patients with chronic myeloid leukemia (CML) can discontinue TKI and remain in treatment-free remission (TFR). The ALLG CML8 study enrolled 40 imatinib-treated patients with undetectable BCR-ABL1 mRNA (approximately MR4.5 ). Molecular relapse was defined as detectable BCR-ABL1 on two consecutive tests or any single value >0.1%. With a median follow-up of 8.6 years (range 5.7-11.2 years), 18 patients remain in continuous TFR (45...
October 12, 2018: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
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