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Molecular test

Xinbo Xu, Heng Liu, Neil Gross, Dongmin Wei, Ye Qian, Wenming Li, Peng Wei, Guojun Li, Fenghua Zhang, Zheng Yang, Dapeng Lei, Xinliang Pan
Hypopharyngeal cancer (HC) is the most common subset of head and neck cancers. These tumors often have an aggressive clinical outcome characterized by local invasion and regional nodal metastasis. Upregulated miRNAs might be useful as biomarkers for prognosis and molecular targets for these tumors. We determined tumor expression of candidate miRNAs using microarray in 8 HC patients and validated in 372 HC patients. We also used paired tumorous and mucosal tissue to verify the miRNA expression. Log-rank test and Cox model were used to evaluate the survival; and Harrell's C-index was used to compare concordance of Cox models...
August 10, 2018: Translational Oncology
M Orditura, C M Della Corte, A Diana, V Ciaramella, E Franzese, V Famiglietti, I Panarese, R Franco, A Grimaldi, A Lombardi, M Caraglia, A Santoriello, E Procaccini, E Lieto, E Maiello, F De Vita, F Ciardiello, F Morgillo
Two inhibitors of phosphatidylinositol 3-kinase (PI3K) pathway taselisib, targeting the mutant PI3K-subunit-alpha (PI3KA) and ipatasertib, AKT-inhibitor, are currently under clinical investigation in breast cancer (BC) patients. We have previously demonstrated the anti-tumor efficacy of these anti-PI3K/AKT-inibitors in combination with anti-microtubule drugs in human BC cell lines, through a complete cytoskeleton disorganization. In this work, we generated ex-vivo three-dimensional (3D) cultures from human BC as a model to test drug efficacy and to identify new molecular biomarkers for selection of BC patients suitable for anti-PI3K/AKT-inibitors treatment...
August 4, 2018: Breast: Official Journal of the European Society of Mastology
Chao-Yuan Huang, Feng-Shu Hsieh, Cheng-Yi Wang, Li-Ju Chen, Shih-Shin Chang, Ming-Hsien Tsai, Man-Hsin Hung, Chiung-Wen Kuo, Chi-Ting Shih, Tzu-I Chao, Kuen-Feng Chen
AIM: Palbociclib is an oral cyclin-dependent kinase 4/6 inhibitor, which is efficacious in treating breast cancer. Currently, there are numerous active clinical trials testing palbociclib alone or in combination with other medications for treating various types of malignancies. Here, we evaluated the anti-cancer effect of palbociclib in combination with radiation therapy (RT) for treating human hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) and addressed the molecular mechanism behind the combination therapy...
August 10, 2018: European Journal of Cancer
Liang Chen, Jie Ren, Xiao Chen, Kai Chen, Man Rao, Ningning Zhang, Wenhua Yu, Jiangping Song
BACKGROUND: Dystrophin gene defects are the pathogenic molecular basis of Becker muscular dystrophy (BMD), characterised by skeletal myopathy and cardiomyopathy. Because of the broad phenotype spectrum, it was difficult to use the traditional diagnostic method to achieve an early accurate diagnosis of BMD-associated cardiomyopathy. METHODS: We applied an in-house gene panel testing and a gene-filtering strategy to investigate the genetic defect in a four-generation family with 73 members...
July 26, 2018: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
Long Sun, Juan Xu, Fang He
OBJECTIVES: Escherichia coli is the leading cause of urinary tract infections worldwide. Carbapenemase producing clinical isolates leave few therapeutic options. Here we report the draft genome sequence of a NDM-5, CTX-M-15, and OXA-1 co-producing E. coli ST167: a multidrug-resistant strain isolated from urine sample of a male hospitalized patient diagnosed with intracranial injury from a traffic accident. METHODS: The genome of E. coli ECWJ1 was sequenced using the Illumina HiSeq™ 4000 platform...
August 10, 2018: Journal of Global Antimicrobial Resistance
Wen-Qiang Cui, Wen-Shan Sun, Fei Xu, Xue-Ming Hu, Wei Yang, Yang Zhou, Li-Xia Du, Wen-Wen Zhang, Qi-Liang Mao-Ying, Wen-Li Mi, Yu-Xia Chu, Yan-Qing Wang
Acupoint catgut embedding (ACE) is a widely used traditional Chinese medicine method to manage various diseases, including chronic inflammatory pain. We sought to assess the possible analgesic effects of ACE in comparison to electroacupuncture (EA) and to study the analgesic mechanisms of ACE in a rat model of inflammatory pain induced by injection of complete Freund's adjuvant (CFA) into the hind paw of rats. The von Frey, radiant heat, and gait analysis tests were performed to evaluate the analgesic effects of ACE and EA, and western blot and immunohistochemistry assays were carried out to determine the molecular mechanisms of ACE...
August 10, 2018: Journal of Pain: Official Journal of the American Pain Society
Lívia Pinto Heckert Bastos, Carlos Wanderlei Piler de Carvalho, Edwin Elard Garcia-Rojas
The aim of this study was to evaluate the influence of some parameters (pH, NaCl, and ratio of biopolymers) on the formation of the complex coacervates of lactoferrin and sodium alginate. Different ratios of lactoferrin:sodium alginate were tested (1:1, 1:2, 1:4,1:8, 2:1, 4:1, and 8:1) at pH levels ranging from 2.0 to 7.0 with different concentrations of NaCl (0, 50, 100, 150, and 200 mM). Sodium alginate has a molecular weight of 138 kDa ± 0.07. The ratio of 8:1(lactoferrin: sodium alginate) at a pH of 4...
August 10, 2018: International Journal of Biological Macromolecules
Yana Pestova, Olga Byadovskaya, Alexander Kononov, Alexander Sprygin
In this paper, we report on the development of a real time high-resolution melting (HRM) PCR assay for detection and differentiation among sheep pox virus (SPPV), goat pox virus (GTPV), field isolates and vaccine strains of lumpy skin disease virus (LSDV) based on high-resolution melting curve analysis of their target PCR amplicons. A 111 bp region of LSDV010 ORF, which harbors unique genetic differences for each of these viral species, was selected as the PCR target in this study. During the validation of this assay using DNA from clinical isolates originated from naturally infected animals from the different geographic locations and reference strains, the obtained PCR amplicons demonstrated that the melting temperature picks were specific for each tested viral species, i...
August 10, 2018: Molecular and Cellular Probes
Anthony P Berger, Bradley A Ford, Zoe Brown-Joel, Bridget E Shields, Misha Rosenbach, Karolyn A Wanat
As discussed in Part I, angioinvasive fungal infections pose a significant risk to immunocompromised and immunocompetent patients alike,1 with potential for severe morbidity and high mortality. Part I of this CME focused on the epidemiology and clinical presentation of these infections; Part II will discuss diagnosis, management, and potential complications of these infections. The mainstay diagnostic tests (positive tissue culture with histologic confirmation) are often supplemented with serum biomarker assays and molecular testing (e...
August 10, 2018: Journal of the American Academy of Dermatology
Mei-Hui Wang, Chuan-Yi Chien, Hung-Man Yu, Ping-Yen Wang, Wuu-Jyh Lin
Many biochemical tests detecting the presence of liver disease are not liver-specific and may be abnormal in non-hepatic conditions. Asialoglycoprotein receptor (ASGPR) is a hepatocyte-specific receptor for Gal/GalNAc-terminated glycopeptide or glycoprotein. The number of these receptors decreases in patients with chronic liver diseases. Here, we aimed to evaluate the use of 111 In-hexavalent lactoside, a known ASGPR imaging biomarker, as a more sensitive probe to detect small changes in liver reserve in animal models of chronic liver injury...
August 13, 2018: Molecular Pharmaceutics
Omer Faruk Karatas
BACKGROUND: Laryngeal cancer is a frequent cause of cancer-associated mortality worldwide with an overall poor prognosis along with high mortality rates. Therefore, comprehensive investigation of underlying molecular mechanisms of laryngeal carcinogenesis remains an important problem. METHODS: In this study, proliferative and apoptotic features of Hep-2 cells overexpressing microRNA-33a (miR-33a) were evaluated and in silico analysis along with literature search was used to find putative targets of miR-33a...
August 13, 2018: Head & Neck
Yamila P Cardoso, Juan J Rosso, Ezequiel Mabragaña, Mariano González-Castro, Matías Delpiani, Esteban Avigliano, Sergio Bogan, Raphael Covain, Nahuel F Schenone, Juan M Díaz de Astarloa
With an estimate of around 9,000 species, the Neotropical region hosts the greatest diversity of freshwater fishes of the world. Genetic surveys have the potential to unravel isolated and unique lineages and may result in the identification of undescribed species, accelerating the cataloguing of extant biodiversity. In this paper, molecular diversity within the valuable and widespread Neotropical genus Hoplias was assessed by means of DNA Barcoding. The geographic coverage spanned 40 degrees of latitude from French Guiana to Argentina...
2018: PloS One
Nayara B da Silva, Naomi S Taus, Wendell C Johnson, Anabela Mira, Leonhard Schnittger, Jessica D M Valente, Odilon Vidotto, Hayley E Masterson, Thállitha S W J Vieira, Massaro W Ueti, Rafael F C Vieira
Anaplasma marginale, the causative agent of bovine anaplasmosis, is a tick-borne bacterium that causes significant economic losses for cattle industries and is increasingly being detected in other animal species. Rhipicephalus microplus is the main vector of this bacterium and may be found parasitizing small ruminants. In northeastern Brazil, multispecies grazing is a common family subsistence practice on smallholder farms possibly facilitating interspecies transmission of pathogens. Considering that A. marginale infection has been previously molecularly described in sheep, this study has aimed to estimate the prevalence of A...
2018: PloS One
Patrick Keller, Isabel Freund, Virginie Marchand, Guillaume Bec, Raven Huang, Yuri Motorin, Tatjana Eigenbrod, Alexander Dalpke, Mark Helm
Sensing of nucleic acids for molecular discrimination between self and non-self is a challenging task for the innate immune system. RNA acts as a potent stimulus for pattern recognition receptors including in particular human Toll-like receptor 7 (TLR7). Certain RNA modifications limit potentially harmful self-recognition of endogenous RNA. Previous studies had identified the 2'-O-methylation of guanosine 18 (Gm18) within tRNAs as an antagonist of TLR7 leading to an impaired immune response. However, human tRNALys3 was non-stimulatory despite lacking Gm18...
August 8, 2018: Nucleic Acids Research
Lei An, Yuchen Sun, Wei Zhang, Xiaolong Huang, Rui Xue, Youzhi Zhang, Yousheng Wang
Recently, dietary intervention has been considered as a prospective strategy in delaying age-related cognitive dysfunction and brain plasticity degeneration. This study explored the effect of walnut diets (6% and 9%, 8 weeks) on cognitive behavior, hippocampal neurogenesis and the neurotrophic signaling pathway in d-galactose (d-gal) model rats. Behavioral tests showed that walnut diets significantly reversed spatial memory loss in the Morris water test, locomotor activity deficiency in an open field test, and a recognition behavior reduction in a novel object recognition task...
August 13, 2018: Food & Function
Minkyo Lee, Min-Goo Seo, Seung-Hun Lee, In-Ohk Ouh, Young-Hoan Kim, Joong-Kew Kim, Youn-Kyoung Goo, Man-Hee Rhee, Tae-Hwan Kim, Oh-Deog Kwon, Dongmi Kwak
The purpose of this study was to assess tick-borne pathogenic infections in 42 wild Korean water deer (KWD) and 26 farmed elk in the Gyeongbuk and Gangwon Provinces of Korea. Among the 42 wild KWD tested, the eighteen (42.9%) and five (11.9%) samples tested positive for Anaplasma phagocytophilum and A. bovis, respectively, by PCR and DNA sequencing. All positive samples were only from wild KWD. All samples were negative for other tick-borne pathogens tested. Detected 16S rRNA sequences of A. phagocytophilum and A...
August 10, 2018: Journal of Veterinary Medical Science
Long Zhao, Xiaoping Tang, Renguo Luo, Jie Duan, Yuanchuan Wang, Binbin Yang
Backgoud Glioma is a type of tumor that start in the glial cells of brain and spine. However,the underlying molecular mechanisms of miRNAs dysregulation in glioma initiation and progression is largely unclear. Objective To further understand the molecular mechanism of miR-490-5P functions and how miR-490 regulated CCND1 function. Methods The expression of miR-490-5P in glioma tissues and cells were measured by qRT-PCR and ISH. Cell transfection is responsible for miR-490-5P overexpression and knockdown. CCK-8and clone formation assay are applicable to examine the capacity of glioma cells proliferation...
August 13, 2018: Current Neurovascular Research
Duraiswamy Basavan, Nehru Sai Suresh Chalichem, Suresh K Mohan Kumar
As the global aged population is increasing tremendously, time has come to concentrate on tail end life stage diseases. Alzheimer's disease (AD) is one such disease whose origin is an enigmatic, impacts later stage of life drastically due to irreparable damage of cognition. The characteristic feature of the disease is the accumulation of neurotoxic amyloid-beta (Aβ) and hyper phosphorylated Tau protein as fibrillary tangles. Several research reports obtained from in vitro and in vivo studies proved the efficacy of herbals in culminating neurodegenerative changes of AD by acting through various molecular pathways at various stages...
August 12, 2018: Current Drug Targets
Chris Hoi Houng Chan, Sara Diab, Kayla Moody, O Howard Frazier, Luiz C Sampaio, Charles D Fraser, Jun Teruya, Iki Adachi
Development of pediatric ventricular assist devices (VADs) has significantly lagged behind that of adult devices. This frustrating reality is reflected by the fact that the Berlin Heart EXCOR VAD is currently the only approved pediatric-specific device in the USA. An alternative option is an off-label use of adult continuous-flow VADs, such as HeartMate II (HMII), which inevitably causes patient-device size mismatch in small children. We sought to conduct in vitro hemocompatibility testing in a pediatric flow condition, with a specific aim to provide benchmark values for future pediatric device development...
August 12, 2018: Artificial Organs
G Kalayci, R C Ozturk, E Capkin, I Altinok
We tested for reproductive isolation between Salmo trutta abanticus, S.t. labrax, S.t. caspius and S.t. fario by conducting crosses to produce F1 and F2 offspring. We also estimated the extent of genetic divergence between all three entities by examining sequence variation across the coI, d-loop and cytb mitochondrial genes. All of the F1 cross-types were successfully produced. After 2 years of culturing, F2 generation were produced as well. Fertilization, hatching and survival rates and hatching performance of F1 and F2 generations were evaluated...
August 12, 2018: Journal of Fish Biology
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